BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147406.5 - phase: 0 /pseudo
(99 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LEU1_BACST (Q8RL85) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 37 0.007
LEU1_ANASP (P48575) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 35 0.032
LEU1_MICAE (P94907) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 33 0.094
LEU1_PYRFU (Q8U2A2) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 32 0.21
LEU1_GLOVI (Q7NI93) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 32 0.27
KI47_MOUSE (Q8VDM1) Protein KIAA1847 homolog 31 0.47
LEU1_SYNY3 (P48576) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 31 0.61
LEU1_SOYBN (Q39891) Probable 2-isopropylmalate synthase (EC 2.3.... 30 1.0
LEU1_CAUCR (Q9A823) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 30 1.0
LEU1_BACSU (P94565) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 30 1.0
LEU1_AQUAE (O67862) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 30 1.0
KI47_HUMAN (Q8N5A5) Protein KIAA1847 30 1.0
SERC_CAEEL (P91856) Probable phosphoserine aminotransferase (EC ... 29 1.8
POLG_ENMG3 (P32540) Genome polyprotein [Contains: Coat protein V... 29 1.8
POLG_EMCVD (P17594) Genome polyprotein [Contains: Coat protein V... 29 1.8
POLG_EMCVB (P17593) Genome polyprotein [Contains: Coat protein V... 29 1.8
POLG_EMCV (P03304) Genome polyprotein [Contains: Coat protein VP... 29 1.8
LEU1_BACAN (Q81T68) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 29 1.8
T2R4_PONPY (Q645U4) Taste receptor type 2 member 4 (T2R4) 29 2.3
LEU1_SYNEL (Q8DJ32) 2-isopropylmalate synthase (EC 2.3.3.13) (Al... 29 2.3
>LEU1_BACST (Q8RL85) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 515
Score = 37.4 bits (85), Expect = 0.007
Identities = 20/50 (40%), Positives = 30/50 (60%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGKN G HA+R R+++ G+ + D + V+F E ADK+K IT
Sbjct: 326 VLGKNSGRHALRYRVEEL-----GYTLSDEEINQLFVRFKELADKKKDIT 370
>LEU1_ANASP (P48575) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 531
Score = 35.0 bits (79), Expect = 0.032
Identities = 20/50 (40%), Positives = 30/50 (60%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK+ G +A R RL++ GFE+ + + VKF E ADK+K I+
Sbjct: 344 VLGKHSGRNAFRTRLKEL-----GFELSETELNKAFVKFKEVADKKKEIS 388
>LEU1_MICAE (P94907) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 533
Score = 33.5 bits (75), Expect = 0.094
Identities = 19/50 (38%), Positives = 29/50 (58%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK G +A R RLQ+ GFE+ + + ++F E AD++K IT
Sbjct: 345 VLGKLSGRNAFRSRLQEL-----GFELSETELNNAFIQFKEMADRKKEIT 389
>LEU1_PYRFU (Q8U2A2) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 499
Score = 32.3 bits (72), Expect = 0.21
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK+ G HA R++L++ G+ + + + KF E AD++K +T
Sbjct: 327 VLGKHSGRHAFRKKLEEM-----GYRLSEEEINRLFAKFKEIADRKKGLT 371
>LEU1_GLOVI (Q7NI93) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 538
Score = 32.0 bits (71), Expect = 0.27
Identities = 18/50 (36%), Positives = 29/50 (58%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK+ G +A R RL + G+E+ D D ++F E ADK+K ++
Sbjct: 342 VLGKHSGRNAFRTRLVEL-----GYELGDADLNRAFLRFKELADKKKTVS 386
>KI47_MOUSE (Q8VDM1) Protein KIAA1847 homolog
Length = 511
Score = 31.2 bits (69), Expect = 0.47
Identities = 14/25 (56%), Positives = 16/25 (64%)
Query: 59 IMDNDNGFYMVKFDEAADKEKVITG 83
I D DNG+Y VKFD KE V+ G
Sbjct: 237 ITDVDNGYYTVKFDSLLLKEAVVEG 261
>LEU1_SYNY3 (P48576) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 533
Score = 30.8 bits (68), Expect = 0.61
Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK G +A RL++ GF++ D + ++F E ADK K IT
Sbjct: 344 VLGKLSGRNAFGTRLKEL-----GFDLSDTELNNAFIRFKEVADKRKEIT 388
>LEU1_SOYBN (Q39891) Probable 2-isopropylmalate synthase (EC
2.3.3.13) (Alpha-isopropylmalate synthase) (Alpha-IPM
synthetase) (Late nodulin 56) (N-56)
Length = 565
Score = 30.0 bits (66), Expect = 1.0
Identities = 17/61 (27%), Positives = 34/61 (54%), Gaps = 5/61 (8%)
Query: 22 VRHGRMQFVVKLLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVI 81
+ H R + +LGK G A+R+RL++ G+++ +++ +F A+K+KV+
Sbjct: 358 IGHKRTTRIGIVLGKLSGSQALRKRLEEL-----GYDLKEDEVDSVFWQFKAMAEKKKVV 412
Query: 82 T 82
T
Sbjct: 413 T 413
>LEU1_CAUCR (Q9A823) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 524
Score = 30.0 bits (66), Expect = 1.0
Identities = 17/47 (36%), Positives = 26/47 (55%), Gaps = 5/47 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEK 79
++GK+ G HA RE+L+ G+E+ N +F E ADK+K
Sbjct: 337 VMGKHSGRHAFREKLKAL-----GYELGQNALNDAFGRFKELADKKK 378
>LEU1_BACSU (P94565) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 518
Score = 30.0 bits (66), Expect = 1.0
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK+ G HA ++RL T GF+ + + F E +K+K IT
Sbjct: 326 VLGKHSGRHAFKDRL-----TALGFQFDSEEINKFFTMFKELTEKKKEIT 370
>LEU1_AQUAE (O67862) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 520
Score = 30.0 bits (66), Expect = 1.0
Identities = 16/49 (32%), Positives = 27/49 (54%), Gaps = 5/49 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVI 81
+LGK+ G HA++ RL++ GF+ + KF E AD++K +
Sbjct: 328 VLGKHSGRHALKRRLEEL-----GFKFTKEELDRIFEKFKELADRKKEV 371
>KI47_HUMAN (Q8N5A5) Protein KIAA1847
Length = 531
Score = 30.0 bits (66), Expect = 1.0
Identities = 13/25 (52%), Positives = 16/25 (64%)
Query: 59 IMDNDNGFYMVKFDEAADKEKVITG 83
I D DNG+Y VKFD +E V+ G
Sbjct: 238 ITDVDNGYYTVKFDSLLLREAVVEG 262
>SERC_CAEEL (P91856) Probable phosphoserine aminotransferase (EC
2.6.1.52) (PSAT)
Length = 370
Score = 29.3 bits (64), Expect = 1.8
Identities = 22/69 (31%), Positives = 32/69 (45%), Gaps = 6/69 (8%)
Query: 8 YPRFISNNQSFNNYVRHG--RMQFVVKLLGKNLGYHAMRE-RLQKTWRTQGGFEIMDNDN 64
Y I+NN +N G V+K + G A+ E LQK+ ++I+DN N
Sbjct: 229 YKEMIANNSLYNTPPTGGIYTTNLVLKWIKSKGGLQAIYELNLQKSGMI---YDIIDNSN 285
Query: 65 GFYMVKFDE 73
GFY D+
Sbjct: 286 GFYHCAVDK 294
>POLG_ENMG3 (P32540) Genome polyprotein [Contains: Coat protein
VP4 (P1A); Coat protein VP2 (P1B); Coat protein VP3
(P1C); Coat protein VP1 (P1D)] (Fragment)
Length = 901
Score = 29.3 bits (64), Expect = 1.8
Identities = 12/27 (44%), Positives = 19/27 (69%)
Query: 64 NGFYMVKFDEAADKEKVITGGPWLIFD 90
NGFY++K+DE E+++T G +FD
Sbjct: 29 NGFYLLKYDEEWYPEELLTDGEDDVFD 55
>POLG_EMCVD (P17594) Genome polyprotein [Contains: Coat protein
VP4 (Rho); Coat protein VP2 (Beta); Coat protein VP3
(Gamma); Coat protein VP1 (Alpha); Picornain 2A (EC
3.4.22.29) (Core protein P2A) (G); Core protein P2B
(I); Core protein P2C (C); Core pr
Length = 2292
Score = 29.3 bits (64), Expect = 1.8
Identities = 12/27 (44%), Positives = 19/27 (69%)
Query: 64 NGFYMVKFDEAADKEKVITGGPWLIFD 90
NGFY++K+DE E+++T G +FD
Sbjct: 29 NGFYLLKYDEEWYPEELLTDGEDDVFD 55
>POLG_EMCVB (P17593) Genome polyprotein [Contains: Coat protein
VP4 (Rho); Coat protein VP2 (Beta); Coat protein VP3
(Gamma); Coat protein VP1 (Alpha); Picornain 2A (EC
3.4.22.29) (Core protein P2A) (G); Core protein P2B
(I); Core protein P2C (C); Core pr
Length = 2292
Score = 29.3 bits (64), Expect = 1.8
Identities = 12/27 (44%), Positives = 19/27 (69%)
Query: 64 NGFYMVKFDEAADKEKVITGGPWLIFD 90
NGFY++K+DE E+++T G +FD
Sbjct: 29 NGFYLLKYDEEWYPEELLTDGEDDVFD 55
>POLG_EMCV (P03304) Genome polyprotein [Contains: Coat protein VP4
(Rho); Coat protein VP2 (Beta); Coat protein VP3
(Gamma); Coat protein VP1 (Alpha); Picornain 2A (EC
3.4.22.29) (Core protein P2A) (G); Core protein P2B
(I); Core protein P2C (C); Core pro
Length = 2290
Score = 29.3 bits (64), Expect = 1.8
Identities = 12/27 (44%), Positives = 19/27 (69%)
Query: 64 NGFYMVKFDEAADKEKVITGGPWLIFD 90
NGFY++K+DE E+++T G +FD
Sbjct: 29 NGFYLLKYDEEWYPEELLTDGEDDVFD 55
>LEU1_BACAN (Q81T68) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 506
Score = 29.3 bits (64), Expect = 1.8
Identities = 15/50 (30%), Positives = 28/50 (56%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK+ G HA E++++ G+E +++ F + AD++K IT
Sbjct: 326 VLGKHSGRHAFTEKMKEL-----GYEFTNDERDAVFEAFKKLADRKKEIT 370
>T2R4_PONPY (Q645U4) Taste receptor type 2 member 4 (T2R4)
Length = 299
Score = 28.9 bits (63), Expect = 2.3
Identities = 16/62 (25%), Positives = 29/62 (45%), Gaps = 12/62 (19%)
Query: 8 YPRFIS--NNQSFNNY----------VRHGRMQFVVKLLGKNLGYHAMRERLQKTWRTQG 55
+P ++ NN SFN + V +QF++ + +L H++R +QK +
Sbjct: 156 FPELVTKRNNTSFNTHEGILSLVVSLVLSSSLQFIINVTSASLLIHSLRRHIQKMQKNAT 215
Query: 56 GF 57
GF
Sbjct: 216 GF 217
>LEU1_SYNEL (Q8DJ32) 2-isopropylmalate synthase (EC 2.3.3.13)
(Alpha-isopropylmalate synthase) (Alpha-IPM synthetase)
Length = 535
Score = 28.9 bits (63), Expect = 2.3
Identities = 17/50 (34%), Positives = 28/50 (56%), Gaps = 5/50 (10%)
Query: 33 LLGKNLGYHAMRERLQKTWRTQGGFEIMDNDNGFYMVKFDEAADKEKVIT 82
+LGK G +A RL++ GFE+ + + ++F + ADK+K IT
Sbjct: 349 VLGKLSGRNAFATRLREL-----GFELSETELNKAFLRFKDLADKKKEIT 393
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.327 0.141 0.460
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,646,459
Number of Sequences: 164201
Number of extensions: 476987
Number of successful extensions: 1194
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 1182
Number of HSP's gapped (non-prelim): 32
length of query: 99
length of database: 59,974,054
effective HSP length: 75
effective length of query: 24
effective length of database: 47,658,979
effective search space: 1143815496
effective search space used: 1143815496
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC147406.5