BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147364.8 + phase: 0
(954 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DJBC_MOUSE (Q9QYI4) DnaJ homolog subfamily B member 12 (mDJ10) 73 4e-12
DJBC_HUMAN (Q9NXW2) DnaJ homolog subfamily B member 12 71 1e-11
DNJ1_DROME (Q24133) DnaJ protein homolog 1 (DROJ1) 67 3e-10
DNAJ_METTE (Q9UXR9) Chaperone protein dnaJ (Heat shock protein 40) 67 3e-10
HLJ1_YEAST (P48353) HLJ1 protein 66 5e-10
DNJH_SYNY3 (P50027) DnAJ-like protein slr0093 66 5e-10
DNAJ_METMA (P35515) Chaperone protein dnaJ 66 5e-10
DNAJ_ERYRH (Q05646) Chaperone protein dnaJ 65 1e-09
DJB9_RAT (P97554) DnaJ homolog subfamily B member 9 (Microvascul... 64 2e-09
DNAJ_DEIRA (Q9RUG2) Chaperone protein dnaJ 63 3e-09
DJB4_HUMAN (Q9UDY4) DnaJ homolog subfamily B member 4 (Heat shoc... 63 3e-09
DNAJ_HELPY (O25890) Chaperone protein dnaJ 62 7e-09
DJB9_HUMAN (Q9UBS3) DnaJ homolog subfamily B member 9 (Microvasc... 62 9e-09
DJB4_MOUSE (Q9D832) DnaJ homolog subfamily B member 4 61 1e-08
DNAJ_HELPJ (Q9ZJQ2) Chaperone protein dnaJ 60 2e-08
DNAJ_CLOAB (P30725) Chaperone protein dnaJ 60 2e-08
DJB9_MOUSE (Q9QYI6) DnaJ homolog subfamily B member 9 (mDJ7) 60 2e-08
DJB5_MOUSE (O89114) DnaJ homolog subfamily B member 5 (Heat shoc... 60 2e-08
DNJ2_MYCLE (Q49762) Chaperone protein dnaJ2 60 4e-08
DNAJ_LISMO (Q9S5A3) Chaperone protein dnaJ 60 4e-08
>DJBC_MOUSE (Q9QYI4) DnaJ homolog subfamily B member 12 (mDJ10)
Length = 376
Score = 72.8 bits (177), Expect = 4e-12
Identities = 58/172 (33%), Positives = 78/172 (44%), Gaps = 44/172 (25%)
Query: 1 MDCNKEEALRAKEIAEKKMENRDFAGARKFALKAQRLYP------VLENIAQMLVVCDVH 54
M+ NK+EA R IA K +++ A +F KAQRLYP ++E++ Q H
Sbjct: 1 MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESLNQKPQSTGDH 60
Query: 55 C-------------------SAEQKVFGDEI-------------------NWYGILQLER 76
SA + G E ++Y IL + R
Sbjct: 61 PQPTDTTHTTTKKAGGTETPSANGEAGGGESAKGYTSEQVAAVKRVKQCKDYYEILGVSR 120
Query: 77 TAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTRYD 128
+A D +KK +RK AL+ HPDKN GA AFK IG A VLS+ EKR +YD
Sbjct: 121 SASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYD 172
>DJBC_HUMAN (Q9NXW2) DnaJ homolog subfamily B member 12
Length = 375
Score = 71.2 bits (173), Expect = 1e-11
Identities = 55/171 (32%), Positives = 78/171 (45%), Gaps = 43/171 (25%)
Query: 1 MDCNKEEALRAKEIAEKKMENRDFAGARKFALKAQRLYP------VLENIAQMLVVC--- 51
M+ NK+EA R IA K +++ A +F KAQRLYP ++E++ Q
Sbjct: 1 MESNKDEAERCISIALKAIQSNQPDRALRFLEKAQRLYPTPRVRALIESLNQKPQTAGDQ 60
Query: 52 ----DVHCSAEQKVFGDEI------------------------------NWYGILQLERT 77
D ++ +K G + ++Y IL + R
Sbjct: 61 PPPTDTTHASHRKAGGTDAPSANGEAGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRG 120
Query: 78 AGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTRYD 128
A D +KK +R+ AL+ HPDKN GA AFK IG A VLS+ EKR +YD
Sbjct: 121 ASDEDLKKAYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYD 171
>DNJ1_DROME (Q24133) DnaJ protein homolog 1 (DROJ1)
Length = 334
Score = 66.6 bits (161), Expect = 3e-10
Identities = 35/62 (56%), Positives = 42/62 (67%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL LER A D IKK +RK AL+ HPDKNK AE FK I EA VLSD++KR
Sbjct: 4 DFYKILGLERKASDDEIKKAYRKLALKYHPDKNKSPQAEERFKEIAEAYEVLSDKKKRDI 63
Query: 127 YD 128
+D
Sbjct: 64 FD 65
>DNAJ_METTE (Q9UXR9) Chaperone protein dnaJ (Heat shock protein 40)
Length = 387
Score = 66.6 bits (161), Expect = 3e-10
Identities = 35/62 (56%), Positives = 41/62 (65%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL L R A IKK +RK AL+ HPD+NK GAE FK I EA VLSD EKR +
Sbjct: 6 DYYEILGLSRDATPEDIKKSYRKLALKYHPDRNKEPGAEEKFKEISEAYAVLSDPEKRAQ 65
Query: 127 YD 128
YD
Sbjct: 66 YD 67
>HLJ1_YEAST (P48353) HLJ1 protein
Length = 224
Score = 65.9 bits (159), Expect = 5e-10
Identities = 33/65 (50%), Positives = 45/65 (68%)
Query: 64 DEINWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREK 123
D+ +Y IL+++R A D+ IKK +RK A++LHPDKN A AFK+I A VLS+ EK
Sbjct: 18 DKHEFYEILKVDRKATDSEIKKAYRKLAIKLHPDKNSHPKAGEAFKVINRAFEVLSNEEK 77
Query: 124 RTRYD 128
R+ YD
Sbjct: 78 RSIYD 82
>DNJH_SYNY3 (P50027) DnAJ-like protein slr0093
Length = 332
Score = 65.9 bits (159), Expect = 5e-10
Identities = 36/63 (57%), Positives = 43/63 (68%), Gaps = 1/63 (1%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNK-FAGAEAAFKLIGEAQRVLSDREKRT 125
++Y IL + +TA +A IKKQFRK AL+ HPDKN AE FK I EA VLSD EKR
Sbjct: 8 DYYQILGVTKTASEAEIKKQFRKLALKYHPDKNPGDKAAEEKFKEISEAYEVLSDPEKRQ 67
Query: 126 RYD 128
+YD
Sbjct: 68 KYD 70
>DNAJ_METMA (P35515) Chaperone protein dnaJ
Length = 389
Score = 65.9 bits (159), Expect = 5e-10
Identities = 34/62 (54%), Positives = 41/62 (65%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL L + + IKK +RK ALQ HPD+NK GAE FK I EA VLSD EKR +
Sbjct: 6 DYYEILGLSKDSSVEDIKKTYRKLALQYHPDRNKEPGAEEKFKEISEAYAVLSDAEKRAQ 65
Query: 127 YD 128
YD
Sbjct: 66 YD 67
>DNAJ_ERYRH (Q05646) Chaperone protein dnaJ
Length = 370
Score = 64.7 bits (156), Expect = 1e-09
Identities = 33/65 (50%), Positives = 44/65 (66%)
Query: 64 DEINWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREK 123
D+ ++Y IL + ++A DA IKK +R+ A + HPD NK GAEA FK + EA VLSD +K
Sbjct: 3 DKRDFYEILGVSKSATDAEIKKAYRQLAKKYHPDINKEDGAEAKFKEVQEAYEVLSDSQK 62
Query: 124 RTRYD 128
R YD
Sbjct: 63 RANYD 67
>DJB9_RAT (P97554) DnaJ homolog subfamily B member 9 (Microvascular
endothelial differentiation gene-1 protein) (Mdg-1)
Length = 222
Score = 63.5 bits (153), Expect = 2e-09
Identities = 30/63 (47%), Positives = 40/63 (62%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
N+Y IL + ++A + IKK F K A++ HPDKNK AEA F+ I EA LSD +R
Sbjct: 26 NYYDILGVPKSASERQIKKAFHKLAMKYHPDKNKSPDAEAKFREIAEAYETLSDANRRKE 85
Query: 127 YDM 129
YD+
Sbjct: 86 YDI 88
>DNAJ_DEIRA (Q9RUG2) Chaperone protein dnaJ
Length = 376
Score = 63.2 bits (152), Expect = 3e-09
Identities = 33/74 (44%), Positives = 43/74 (57%)
Query: 66 INWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRT 125
+++Y +L + RTA IK +RK AL+LHPD+NK GA F + EA VLSD EKR
Sbjct: 1 MDYYELLGVSRTASADEIKSAYRKLALKLHPDRNKEEGAAEKFAQVSEAYSVLSDTEKRA 60
Query: 126 RYDMKLNVNKTAMP 139
YD + MP
Sbjct: 61 HYDRFGSAPGAGMP 74
>DJB4_HUMAN (Q9UDY4) DnaJ homolog subfamily B member 4 (Heat shock
40 kDa protein 1 homolog) (Heat shock protein 40
homolog) (HSP40 homolog)
Length = 337
Score = 63.2 bits (152), Expect = 3e-09
Identities = 33/62 (53%), Positives = 41/62 (65%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL +E+ A D IKK +RK AL+ HPDKNK AE FK + EA VLSD +KR
Sbjct: 4 DYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREI 63
Query: 127 YD 128
YD
Sbjct: 64 YD 65
>DNAJ_HELPY (O25890) Chaperone protein dnaJ
Length = 369
Score = 62.0 bits (149), Expect = 7e-09
Identities = 34/67 (50%), Positives = 45/67 (66%), Gaps = 5/67 (7%)
Query: 65 EINWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAG---AEAAFKLIGEAQRVLSDR 121
E+++Y IL++E+ + IKK +RK AL+ HPD+N AG AE FKLI EA VLSD
Sbjct: 2 ELSYYEILEVEKHSNQETIKKSYRKLALKYHPDRN--AGDKEAEEKFKLINEAYGVLSDE 59
Query: 122 EKRTRYD 128
+KR YD
Sbjct: 60 KKRALYD 66
>DJB9_HUMAN (Q9UBS3) DnaJ homolog subfamily B member 9
(Microvascular endothelial differentiation gene-1
protein) (Mdg-1) (UNQ743/PRO1471)
Length = 223
Score = 61.6 bits (148), Expect = 9e-09
Identities = 41/129 (31%), Positives = 59/129 (44%), Gaps = 10/129 (7%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL + ++A + IKK F K A++ HPDKNK AEA F+ I EA LSD +R
Sbjct: 26 SYYDILGVPKSASERQIKKAFHKLAMKYHPDKNKSPDAEAKFREIAEAYETLSDANRRKE 85
Query: 127 YDMKLNVNKTAMPPRSNQPKVPTNFNSATKNNVRTNF-------TNSNTQQPPQQQNKQP 179
YD + +A Q ++F + N F N NT + +N
Sbjct: 86 YD---TLGHSAFTSGKGQRGSGSSFEQSFNFNFDDLFKDFGFFGQNQNTGSKKRFENHFQ 142
Query: 180 PQQQNGVRR 188
+Q G R
Sbjct: 143 TRQDGGSSR 151
>DJB4_MOUSE (Q9D832) DnaJ homolog subfamily B member 4
Length = 337
Score = 61.2 bits (147), Expect = 1e-08
Identities = 31/62 (50%), Positives = 41/62 (66%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL +++ A D +KK +RK AL+ HPDKNK AE FK + EA VLSD +KR
Sbjct: 4 DYYHILGIDKGATDEDVKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREI 63
Query: 127 YD 128
YD
Sbjct: 64 YD 65
>DNAJ_HELPJ (Q9ZJQ2) Chaperone protein dnaJ
Length = 369
Score = 60.5 bits (145), Expect = 2e-08
Identities = 33/67 (49%), Positives = 44/67 (65%), Gaps = 5/67 (7%)
Query: 65 EINWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAG---AEAAFKLIGEAQRVLSDR 121
E+++Y IL++E+ + IKK +RK AL+ HPD+N AG AE FKLI EA VL D
Sbjct: 2 ELSYYEILEVEKHSNQETIKKSYRKLALKYHPDRN--AGDKEAEEKFKLINEAYGVLGDE 59
Query: 122 EKRTRYD 128
+KR YD
Sbjct: 60 KKRALYD 66
>DNAJ_CLOAB (P30725) Chaperone protein dnaJ
Length = 374
Score = 60.5 bits (145), Expect = 2e-08
Identities = 32/63 (50%), Positives = 42/63 (65%), Gaps = 1/63 (1%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNK-FAGAEAAFKLIGEAQRVLSDREKRT 125
++Y +L LE+ A D IKK FRK A++ HPDKN+ AE FK I EA +VLSD +K+
Sbjct: 5 DYYEVLGLEKGASDDEIKKAFRKLAIKYHPDKNRGNKEAEEKFKEINEAYQVLSDPDKKA 64
Query: 126 RYD 128
YD
Sbjct: 65 NYD 67
>DJB9_MOUSE (Q9QYI6) DnaJ homolog subfamily B member 9 (mDJ7)
Length = 222
Score = 60.5 bits (145), Expect = 2e-08
Identities = 29/62 (46%), Positives = 38/62 (60%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL + ++A + IKK F K A++ HPDKNK AEA F+ I EA LSD R
Sbjct: 26 SYYDILGVPKSASERQIKKAFHKLAMKYHPDKNKSPDAEAKFREIAEAYETLSDANSRKE 85
Query: 127 YD 128
YD
Sbjct: 86 YD 87
>DJB5_MOUSE (O89114) DnaJ homolog subfamily B member 5 (Heat shock
protein Hsp40-3) (Heat shock protein cognate 40) (Hsc40)
Length = 348
Score = 60.5 bits (145), Expect = 2e-08
Identities = 32/62 (51%), Positives = 40/62 (63%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y IL + A + IKK +RK AL+ HPDKNK AE FK I EA VLSD +KR+
Sbjct: 4 DYYKILGIPSGANEDEIKKAYRKMALKYHPDKNKEPNAEEKFKEIAEAYDVLSDPKKRSL 63
Query: 127 YD 128
YD
Sbjct: 64 YD 65
>DNJ2_MYCLE (Q49762) Chaperone protein dnaJ2
Length = 378
Score = 59.7 bits (143), Expect = 4e-08
Identities = 31/63 (49%), Positives = 40/63 (63%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++YG+L + R A DA IK+ +RK A +LHPD N A+A FK I A VLSD EKR
Sbjct: 4 DYYGLLGVSRNASDADIKRAYRKLARELHPDINPDEAAQAKFKEISMAYEVLSDPEKRRI 63
Query: 127 YDM 129
D+
Sbjct: 64 VDL 66
>DNAJ_LISMO (Q9S5A3) Chaperone protein dnaJ
Length = 377
Score = 59.7 bits (143), Expect = 4e-08
Identities = 29/62 (46%), Positives = 40/62 (63%)
Query: 67 NWYGILQLERTAGDAMIKKQFRKFALQLHPDKNKFAGAEAAFKLIGEAQRVLSDREKRTR 126
++Y +L + ++A IKK +RK + Q HPD NK AGA+ FK I EA LSD +KR +
Sbjct: 5 DYYEVLGISKSASADEIKKAYRKLSKQYHPDINKEAGADEKFKEISEAYEALSDPQKRAQ 64
Query: 127 YD 128
YD
Sbjct: 65 YD 66
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.132 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 121,251,879
Number of Sequences: 164201
Number of extensions: 5505768
Number of successful extensions: 19349
Number of sequences better than 10.0: 348
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 174
Number of HSP's that attempted gapping in prelim test: 18323
Number of HSP's gapped (non-prelim): 737
length of query: 954
length of database: 59,974,054
effective HSP length: 120
effective length of query: 834
effective length of database: 40,269,934
effective search space: 33585124956
effective search space used: 33585124956
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 71 (32.0 bits)
Medicago: description of AC147364.8