BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147179.8 + phase: 0
(307 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CDC4_YEAST (P07834) Cell division control protein 4 33 1.2
NODH_RHITR (P52994) Nodulation protein H (EC 2.8.2.-) 32 2.0
FXLC_MOUSE (Q9EPX5) F-box/LRR-repeat protein 12 (F-box and leuci... 32 2.0
E320_ADE03 (P11321) Early E3 20.1 kDa glycoprotein 32 2.0
FBX2_MOUSE (Q80UW2) F-box only protein 2 32 2.6
FBX2_HUMAN (Q9UK22) F-box only protein 2 32 2.6
FL2_HUMAN (Q9UKC9) F-box/LRR-repeat protein 2 (F-box and leucine... 31 3.4
E320_ADE35 (P15137) Early E3 20.3 kDa glycoprotein 31 4.4
E320_ADE1P (P35768) Early E3 20.3 kDa glycoprotein 31 4.4
E320_ADE1A (P35767) Early E3 20.3 kDa glycoprotein 31 4.4
Y027_RICPR (Q9ZEB7) Hypothetical protein RP027 30 5.8
FXLK_HUMAN (Q96IG2) F-box/LRR-repeat protein 20 (F-box and leuci... 30 5.8
FXLC_HUMAN (Q9NXK8) F-box/LRR-repeat protein 12 (F-box and leuci... 30 5.8
POF1_SCHPO (P87053) F-box/WD-repeat protein pof1 (Skp1-binding p... 30 7.5
FXLK_RAT (Q9QZH7) F-box/LRR-repeat protein 20 (F-box and leucine... 30 7.5
FXLK_MOUSE (Q9CZV8) F-box/LRR-repeat protein 20 (F-box and leuci... 30 7.5
FL2_MOUSE (Q8BH16) F-box/LRR-repeat protein 2 (F-box and leucine... 30 9.8
>CDC4_YEAST (P07834) Cell division control protein 4
Length = 779
Score = 32.7 bits (73), Expect = 1.2
Identities = 17/65 (26%), Positives = 34/65 (52%), Gaps = 3/65 (4%)
Query: 10 DYVKEDRLSDLPNCVLLRILSLLDADEAVKTCTISSRWRHLWKHLPTL---ILCSSHFMR 66
D +K D ++ LP + L+I + L ++ + + +S W + + +L +L S +F+
Sbjct: 268 DNLKRDLITSLPFEISLKIFNYLQFEDIINSLGVSQNWNKIIRKSTSLWKKLLISENFVS 327
Query: 67 IKSFN 71
K FN
Sbjct: 328 PKGFN 332
>NODH_RHITR (P52994) Nodulation protein H (EC 2.8.2.-)
Length = 249
Score = 32.0 bits (71), Expect = 2.0
Identities = 29/93 (31%), Positives = 42/93 (44%), Gaps = 6/93 (6%)
Query: 137 HTLTSLDLCVGPPRTDERLSFPKSLNLPALTTLSLESFA--FCVGDDGCAEPFSALNSLK 194
+TL SL V +T + L F N P +LS+ F DD A A + K
Sbjct: 112 NTLESLRSLVQARQTGQWLKFSPDRNEPPSVSLSVNECEAYFKAADDFHARVKDAFDPSK 171
Query: 195 NLMILYCNVLDAESLCISSVTLTNLTIVGDPVY 227
LMI Y ++L S C+++V L +G P +
Sbjct: 172 LLMIEYQDLLLKPSACLAAV----LAFLGAPAH 200
>FXLC_MOUSE (Q9EPX5) F-box/LRR-repeat protein 12 (F-box and
leucine-rich repeat protein 12) (F-box protein FBL12)
Length = 326
Score = 32.0 bits (71), Expect = 2.0
Identities = 16/44 (36%), Positives = 23/44 (51%), Gaps = 6/44 (13%)
Query: 17 LSDLPNCVLLRILSLLDADEAVKTCTISSRW------RHLWKHL 54
L DLP+ VLL I S L + ++ + RW R LW+H+
Sbjct: 4 LFDLPDLVLLEIFSYLPVRDRIRISRVCHRWKRLVDDRWLWRHV 47
>E320_ADE03 (P11321) Early E3 20.1 kDa glycoprotein
Length = 179
Score = 32.0 bits (71), Expect = 2.0
Identities = 27/111 (24%), Positives = 48/111 (42%), Gaps = 2/111 (1%)
Query: 197 MILYCNVLDAESLCISSVTLT-NLTIVGDPVYYSTVELSTPSLFTFDFVCYEGIPVLKLC 255
+I+ V A I +TL N T+VG P+ + S+ FD +C + P+ +C
Sbjct: 7 LIIASIVTVAHGQTIVHITLGHNHTLVGPPITSEVIWTKLGSVDYFDIICNKTKPIFVIC 66
Query: 256 RSKINLSSVKHVNIEVRMWTDYADASLVLLNWLAELANIKSLTLNHTALKV 306
++ NL+ + I + Y +S N+L + K T +K+
Sbjct: 67 -NRQNLTLINVSKIYNGYYYGYDRSSSQYKNYLVRITQPKLTVPTMTIIKM 116
>FBX2_MOUSE (Q80UW2) F-box only protein 2
Length = 297
Score = 31.6 bits (70), Expect = 2.6
Identities = 17/44 (38%), Positives = 26/44 (58%), Gaps = 1/44 (2%)
Query: 17 LSDLPNCVLLRILSLLDADEAVKTC-TISSRWRHLWKHLPTLIL 59
L++LP +LLR+L+ L A E V+ C + RW+ L P +L
Sbjct: 51 LAELPEPLLLRVLAELPATELVQACRLVCLRWKELVDGAPLWLL 94
>FBX2_HUMAN (Q9UK22) F-box only protein 2
Length = 296
Score = 31.6 bits (70), Expect = 2.6
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 1/44 (2%)
Query: 17 LSDLPNCVLLRILSLLDADEAVKTC-TISSRWRHLWKHLPTLIL 59
L +LP +LLR+L+ L A E V+ C + RW+ L P +L
Sbjct: 47 LDELPEPLLLRVLAALPAAELVQACRLVCLRWKELVDGAPLWLL 90
>FL2_HUMAN (Q9UKC9) F-box/LRR-repeat protein 2 (F-box and
leucine-rich repeat protein 2) (F-box protein FBL2/FBL3)
Length = 423
Score = 31.2 bits (69), Expect = 3.4
Identities = 58/208 (27%), Positives = 78/208 (36%), Gaps = 40/208 (19%)
Query: 20 LPNCVLLRILSLLDADEAVKTCTISSRWRHLWKHLPTLILCSSHFMRIKSFN-------R 72
LP +LLRI S LD + IS W L L S++ RI FN R
Sbjct: 15 LPKELLLRIFSFLDIVTLCRCAQISKAWN-------ILALDGSNWQRIDLFNFQIDVEGR 67
Query: 73 FVSRIL-----CLRDVSTPLHAVDFLCRGVVDPRLLKKVVTYAVS-HNVEKLRVHACCDF 126
V I LR +S C GV D L T+A + N+E L ++ C
Sbjct: 68 VVENISKRCGGFLRKLSLR------GCIGVGDSSL----KTFAQNCRNIEHLNLNGCTKI 117
Query: 127 QHFSSCFF---SCHTLTSLDL--CVGPPRTDERLSFPKSLNLPALTTLSLESFAFCVGDD 181
S+C+ C L LDL CV + + NL L S+ + D
Sbjct: 118 TD-STCYSLSRFCSKLKHLDLTSCVSITNSSLKGISEGCRNLEYLNL----SWCDQITKD 172
Query: 182 GCAEPFSALNSLKNLMILYCNVLDAESL 209
G LK L++ C L+ E+L
Sbjct: 173 GIEALVRGCRGLKALLLRGCTQLEDEAL 200
>E320_ADE35 (P15137) Early E3 20.3 kDa glycoprotein
Length = 181
Score = 30.8 bits (68), Expect = 4.4
Identities = 18/86 (20%), Positives = 38/86 (43%), Gaps = 1/86 (1%)
Query: 218 NLTIVGDPVYYSTVELSTPSLFTFDFVCYEGIPVLKLCRSKINLSSVKHVNIEVRMWTDY 277
N T++G P+ + S+ FD +C + P++ C + NL+ + + + Y
Sbjct: 29 NYTLIGPPITSEVIWTKLGSVDYFDIICNKTKPIIVTCNIQ-NLTLINVSKVYSGYYYGY 87
Query: 278 ADASLVLLNWLAELANIKSLTLNHTA 303
S N+L + +K+ + + A
Sbjct: 88 DRYSSQYRNYLVRVTQLKTTKMPNMA 113
>E320_ADE1P (P35768) Early E3 20.3 kDa glycoprotein
Length = 181
Score = 30.8 bits (68), Expect = 4.4
Identities = 18/86 (20%), Positives = 38/86 (43%), Gaps = 1/86 (1%)
Query: 218 NLTIVGDPVYYSTVELSTPSLFTFDFVCYEGIPVLKLCRSKINLSSVKHVNIEVRMWTDY 277
N T++G P+ + S+ FD +C + P++ C + NL+ + + + Y
Sbjct: 29 NYTLIGPPITSEVIWAKLGSVDYFDIICNKTKPIIVTCNIQ-NLTLINVSKVYSGYYYGY 87
Query: 278 ADASLVLLNWLAELANIKSLTLNHTA 303
S N+L + +K+ + + A
Sbjct: 88 DRYSSQYRNYLVRVTQLKTTKMPNMA 113
>E320_ADE1A (P35767) Early E3 20.3 kDa glycoprotein
Length = 181
Score = 30.8 bits (68), Expect = 4.4
Identities = 24/108 (22%), Positives = 46/108 (42%), Gaps = 2/108 (1%)
Query: 197 MILYCNVLDAESLCISSVTLT-NLTIVGDPVYYSTVELSTPSLFTFDFVCYEGIPVLKLC 255
+IL V A + S+ L N T++G P+ + S+ FD +C + P++ C
Sbjct: 7 LILVSIVTAAHGQTVVSIPLGHNYTLIGPPITSEVIWTKLGSVDYFDIICNKTKPIIVTC 66
Query: 256 RSKINLSSVKHVNIEVRMWTDYADASLVLLNWLAELANIKSLTLNHTA 303
+ NL+ + + + Y S N+L + K+ + + A
Sbjct: 67 NIQ-NLTLINVSKVYSGYYYGYDRYSSQYRNYLVRVTQSKTTKMPNMA 113
>Y027_RICPR (Q9ZEB7) Hypothetical protein RP027
Length = 252
Score = 30.4 bits (67), Expect = 5.8
Identities = 15/35 (42%), Positives = 23/35 (64%)
Query: 87 LHAVDFLCRGVVDPRLLKKVVTYAVSHNVEKLRVH 121
L A D + V DP+LLK+ V Y V+ ++EK R++
Sbjct: 213 LIACDNKGQAVTDPKLLKEFVDYIVNISLEKFRIN 247
>FXLK_HUMAN (Q96IG2) F-box/LRR-repeat protein 20 (F-box and
leucine-rich repeat protein 20) (F-box/LRR-repeat
protein 2-like)
Length = 436
Score = 30.4 bits (67), Expect = 5.8
Identities = 57/205 (27%), Positives = 77/205 (36%), Gaps = 34/205 (16%)
Query: 20 LPNCVLLRILSLLDADEAVKTCTISSRWRHLWKHLPTLILCSSHFMRIKSFN-------R 72
LP +LLRI S LD + +S W L L S++ RI F+ R
Sbjct: 28 LPKELLLRIFSFLDVVTLCRCAQVSRAWN-------VLALDGSNWQRIDLFDFQRDIEGR 80
Query: 73 FVSRIL-----CLRDVSTPLHAVDFLCRGVVDPRLLKKVVTYAVS-HNVEKLRVHACCDF 126
V I LR +S C GV D L T+A + N+E L ++ C
Sbjct: 81 VVENISKRCGGFLRKLSLR------GCLGVGDNALR----TFAQNCRNIEVLNLNGCTKT 130
Query: 127 QHFSSCFFS--CHTLTSLDLCVGPPRTDERLSFPKSLNLPALTTLSLESFAFCVGDDGCA 184
+ S C L LDL T+ L S P L L++ S+ V DG
Sbjct: 131 TDATCTSLSKFCSKLRHLDLASCTSITNMSLK-ALSEGCPLLEQLNI-SWCDQVTKDGIQ 188
Query: 185 EPFSALNSLKNLMILYCNVLDAESL 209
LK L + C L+ E+L
Sbjct: 189 ALVRGCGGLKALFLKGCTQLEDEAL 213
>FXLC_HUMAN (Q9NXK8) F-box/LRR-repeat protein 12 (F-box and
leucine-rich repeat protein 12) (F-box protein FBL12)
Length = 326
Score = 30.4 bits (67), Expect = 5.8
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 6/44 (13%)
Query: 17 LSDLPNCVLLRILSLLDADEAVKTCTISSRW------RHLWKHL 54
L +LP+ VLL I S L + ++ + RW R LW+H+
Sbjct: 4 LVELPDSVLLEIFSYLPVRDRIRISRVCHRWKRLVDDRWLWRHV 47
>POF1_SCHPO (P87053) F-box/WD-repeat protein pof1 (Skp1-binding
protein 1)
Length = 605
Score = 30.0 bits (66), Expect = 7.5
Identities = 16/39 (41%), Positives = 21/39 (53%)
Query: 12 VKEDRLSDLPNCVLLRILSLLDADEAVKTCTISSRWRHL 50
V+ D LS LP + RILS LDA + +S W+ L
Sbjct: 105 VRLDFLSLLPVEISFRILSFLDARSLCQAAQVSKHWKEL 143
>FXLK_RAT (Q9QZH7) F-box/LRR-repeat protein 20 (F-box and
leucine-rich repeat protein 20) (F-box/LRR-repeat
protein 2-like)
Length = 276
Score = 30.0 bits (66), Expect = 7.5
Identities = 57/205 (27%), Positives = 77/205 (36%), Gaps = 34/205 (16%)
Query: 20 LPNCVLLRILSLLDADEAVKTCTISSRWRHLWKHLPTLILCSSHFMRIKSFN-------R 72
LP +LLRI S LD + +S W L L S++ RI F+ R
Sbjct: 28 LPKELLLRIFSFLDVVTLCRCAQVSRAWN-------VLALDGSNWQRIDLFDFQRDIEGR 80
Query: 73 FVSRIL-----CLRDVSTPLHAVDFLCRGVVDPRLLKKVVTYAVS-HNVEKLRVHACCDF 126
V I LR +S C GV D L T+A + N+E L ++ C
Sbjct: 81 VVENISKRCGGFLRKLSLR------GCLGVGDNALR----TFAQNCRNIEVLSLNGCTKT 130
Query: 127 QHFSSCFFS--CHTLTSLDLCVGPPRTDERLSFPKSLNLPALTTLSLESFAFCVGDDGCA 184
+ S C L LDL T+ L S P L L++ S+ V DG
Sbjct: 131 TDATCTSLSKFCSKLRHLDLASCTSITNMSLK-ALSEGCPLLEQLNI-SWCDQVTKDGIQ 188
Query: 185 EPFSALNSLKNLMILYCNVLDAESL 209
LK L + C L+ E+L
Sbjct: 189 ALVRGCGGLKALFLKGCTQLEDEAL 213
>FXLK_MOUSE (Q9CZV8) F-box/LRR-repeat protein 20 (F-box and
leucine-rich repeat protein 20) (F-box/LRR-repeat
protein 2-like)
Length = 436
Score = 30.0 bits (66), Expect = 7.5
Identities = 57/205 (27%), Positives = 77/205 (36%), Gaps = 34/205 (16%)
Query: 20 LPNCVLLRILSLLDADEAVKTCTISSRWRHLWKHLPTLILCSSHFMRIKSFN-------R 72
LP +LLRI S LD + +S W L L S++ RI F+ R
Sbjct: 28 LPKELLLRIFSFLDVVTLCRCAQVSRAWN-------VLALDGSNWQRIDLFDFQRDIEGR 80
Query: 73 FVSRIL-----CLRDVSTPLHAVDFLCRGVVDPRLLKKVVTYAVS-HNVEKLRVHACCDF 126
V I LR +S C GV D L T+A + N+E L ++ C
Sbjct: 81 VVENISKRCGGFLRKLSLR------GCLGVGDNALR----TFAQNCRNIEVLSLNGCTKT 130
Query: 127 QHFSSCFFS--CHTLTSLDLCVGPPRTDERLSFPKSLNLPALTTLSLESFAFCVGDDGCA 184
+ S C L LDL T+ L S P L L++ S+ V DG
Sbjct: 131 TDATCTSLSKFCSKLRHLDLASCTSITNMSLK-ALSEGCPLLEQLNI-SWCDQVTKDGIQ 188
Query: 185 EPFSALNSLKNLMILYCNVLDAESL 209
LK L + C L+ E+L
Sbjct: 189 ALVRGCGGLKALFLKGCTQLEDEAL 213
>FL2_MOUSE (Q8BH16) F-box/LRR-repeat protein 2 (F-box and
leucine-rich repeat protein 2)
Length = 423
Score = 29.6 bits (65), Expect = 9.8
Identities = 56/208 (26%), Positives = 78/208 (36%), Gaps = 40/208 (19%)
Query: 20 LPNCVLLRILSLLDADEAVKTCTISSRWRHLWKHLPTLILCSSHFMRIKSFN-------R 72
LP +LLRI S LD + IS W L L S++ R+ FN R
Sbjct: 15 LPKELLLRIFSFLDIVTLCRCAQISKAWN-------ILALDGSNWQRVDLFNFQTDVEGR 67
Query: 73 FVSRIL-----CLRDVSTPLHAVDFLCRGVVDPRLLKKVVTYAVS-HNVEKLRVHACCDF 126
V I LR +S C GV D L T+A + N+E L ++ C
Sbjct: 68 VVENISKRCGGFLRKLSLR------GCIGVGDSSL----KTFAQNCRNIEHLNLNGCTKI 117
Query: 127 QHFSSCFF---SCHTLTSLDL--CVGPPRTDERLSFPKSLNLPALTTLSLESFAFCVGDD 181
S+C+ C L LDL CV + + NL L S+ + +
Sbjct: 118 TD-STCYSLSRFCSKLKHLDLTSCVSVTNSSLKGISEGCRNLEYLNL----SWCDQITKE 172
Query: 182 GCAEPFSALNSLKNLMILYCNVLDAESL 209
G LK L++ C L+ E+L
Sbjct: 173 GIEALVRGCRGLKALLLRGCTQLEDEAL 200
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.138 0.431
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,516,994
Number of Sequences: 164201
Number of extensions: 1326339
Number of successful extensions: 3485
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 3482
Number of HSP's gapped (non-prelim): 17
length of query: 307
length of database: 59,974,054
effective HSP length: 110
effective length of query: 197
effective length of database: 41,911,944
effective search space: 8256652968
effective search space used: 8256652968
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 65 (29.6 bits)
Medicago: description of AC147179.8