BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147178.12 + phase: 0
(163 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CVRA_PSEPK (Q88CW4) Cell volume regulation protein A homolog 30 1.8
MYBH_DICDI (P34127) Myb-like protein (Fragment) 29 4.1
MNHD_STAAW (P60687) Na(+)/H(+) antiporter subunit D (Mnh complex... 29 4.1
MNHD_STAAU (P60686) Na(+)/H(+) antiporter subunit D (Mnh complex... 29 4.1
MNHD_STAAN (P60685) Na(+)/H(+) antiporter subunit D (Mnh complex... 29 4.1
MNHD_STAAM (P60684) Na(+)/H(+) antiporter subunit D (Mnh complex... 29 4.1
CVRA_ECOLI (P76007) Cell volume regulation protein A 29 4.1
CRCB_COREF (Q8FMR6) Protein crcB homolog 29 4.1
MGE1_HUMAN (Q9HCI5) Melanoma-associated antigen E1 (MAGE-E1 anti... 29 5.4
FOCA_HAEIN (P43756) Probable formate transporter (Formate channel) 29 5.4
FINC_RAT (P04937) Fibronectin precursor (FN) 29 5.4
CTG1_HUMAN (P78358) Cancer/testis antigen 1B (Autoimmunogenic ca... 29 5.4
AQL1_THEAQ (P08594) Aqualysin I precursor (EC 3.4.21.-) 29 5.4
PTCC_ECOLI (P17334) PTS system, N,N'-diacetylchitobiose-specific... 28 7.0
PEND_MOUSE (Q9R155) Pendrin (Sodium-independent chloride/iodide ... 28 9.1
P121_RAT (P52591) Nuclear envelope pore membrane protein POM 121... 28 9.1
CVRA_SHIFL (Q83RQ1) Cell volume regulation protein A 28 9.1
CVRA_ECOL6 (P59239) Cell volume regulation protein A 28 9.1
ACSA_METSO (P27095) Acetyl-coenzyme A synthetase (EC 6.2.1.1) (A... 28 9.1
>CVRA_PSEPK (Q88CW4) Cell volume regulation protein A homolog
Length = 580
Score = 30.4 bits (67), Expect = 1.8
Identities = 34/118 (28%), Positives = 49/118 (40%), Gaps = 26/118 (22%)
Query: 67 WSL--YTLKTTGCGLPPGPGGSIGALEGVS----------YLVVVGIVAWSIYTKTKTGS 114
WSL + L+ G G G GG L+ V+ LVV G + T GS
Sbjct: 184 WSLLGHLLREFGIGTLLGLGGGWLMLQLVNRINLAGGLYPILVVAGGLVMFSLTNALHGS 243
Query: 115 G----------LPNGPF----GLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPA 158
G L N P G+L ++G+++LA + + +V GL +P LPA
Sbjct: 244 GFLAVYLCGLVLGNKPIRSRHGILHMLDGMAWLAQIGMFLVLGLLVTPHDLLPIALPA 301
>MYBH_DICDI (P34127) Myb-like protein (Fragment)
Length = 451
Score = 29.3 bits (64), Expect = 4.1
Identities = 20/80 (25%), Positives = 30/80 (37%), Gaps = 3/80 (3%)
Query: 4 FSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEGSDTNSSPTEIAAIAGGLISTP 63
F P T NH N +++ +++V LK + D SP I + + P
Sbjct: 281 FLPGRTDNAIKNHWNSSMKRVSNNNVHLKSHAIEHSLSSQDNQDSPKSIITSSSPI---P 337
Query: 64 VIGWSLYTLKTTGCGLPPGP 83
+ T TT PP P
Sbjct: 338 TTTTTTTTTSTTLITPPPPP 357
>MNHD_STAAW (P60687) Na(+)/H(+) antiporter subunit D (Mnh complex
subunit D)
Length = 498
Score = 29.3 bits (64), Expect = 4.1
Identities = 32/100 (32%), Positives = 44/100 (44%), Gaps = 18/100 (18%)
Query: 57 GGLIST-PVIGWSLYTLKTTGCGLPPGPGGSIGA-------------LEGVSYLVVVGIV 102
GGLI PV+GWS + + G+PP G G L GV L+ +V
Sbjct: 364 GGLIKRYPVLGWSFFIAALSLAGIPP-LSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVV 422
Query: 103 AWSIYTKTKTG-SGLPNGPFGLLGAVEGLSYLALVAIVVV 141
+S+ G G P G + L V+ + YL +AIV V
Sbjct: 423 LYSVIRIFLQGFFGQPKG-YDLNNKVD-VKYLTTIAIVAV 460
>MNHD_STAAU (P60686) Na(+)/H(+) antiporter subunit D (Mnh complex
subunit D)
Length = 498
Score = 29.3 bits (64), Expect = 4.1
Identities = 32/100 (32%), Positives = 44/100 (44%), Gaps = 18/100 (18%)
Query: 57 GGLIST-PVIGWSLYTLKTTGCGLPPGPGGSIGA-------------LEGVSYLVVVGIV 102
GGLI PV+GWS + + G+PP G G L GV L+ +V
Sbjct: 364 GGLIKRYPVLGWSFFIAALSLAGIPP-LSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVV 422
Query: 103 AWSIYTKTKTG-SGLPNGPFGLLGAVEGLSYLALVAIVVV 141
+S+ G G P G + L V+ + YL +AIV V
Sbjct: 423 LYSVIRIFLQGFFGQPKG-YDLNNKVD-VKYLTTIAIVAV 460
>MNHD_STAAN (P60685) Na(+)/H(+) antiporter subunit D (Mnh complex
subunit D)
Length = 498
Score = 29.3 bits (64), Expect = 4.1
Identities = 32/100 (32%), Positives = 44/100 (44%), Gaps = 18/100 (18%)
Query: 57 GGLIST-PVIGWSLYTLKTTGCGLPPGPGGSIGA-------------LEGVSYLVVVGIV 102
GGLI PV+GWS + + G+PP G G L GV L+ +V
Sbjct: 364 GGLIKRYPVLGWSFFIAALSLAGIPP-LSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVV 422
Query: 103 AWSIYTKTKTG-SGLPNGPFGLLGAVEGLSYLALVAIVVV 141
+S+ G G P G + L V+ + YL +AIV V
Sbjct: 423 LYSVIRIFLQGFFGQPKG-YDLNNKVD-VKYLTTIAIVAV 460
>MNHD_STAAM (P60684) Na(+)/H(+) antiporter subunit D (Mnh complex
subunit D)
Length = 498
Score = 29.3 bits (64), Expect = 4.1
Identities = 32/100 (32%), Positives = 44/100 (44%), Gaps = 18/100 (18%)
Query: 57 GGLIST-PVIGWSLYTLKTTGCGLPPGPGGSIGA-------------LEGVSYLVVVGIV 102
GGLI PV+GWS + + G+PP G G L GV L+ +V
Sbjct: 364 GGLIKRYPVLGWSFFIAALSLAGIPP-LSGFYGKFFIVQSTFERGFYLSGVIVLLSSLVV 422
Query: 103 AWSIYTKTKTG-SGLPNGPFGLLGAVEGLSYLALVAIVVV 141
+S+ G G P G + L V+ + YL +AIV V
Sbjct: 423 LYSVIRIFLQGFFGQPKG-YDLNNKVD-VKYLTTIAIVAV 460
>CVRA_ECOLI (P76007) Cell volume regulation protein A
Length = 578
Score = 29.3 bits (64), Expect = 4.1
Identities = 30/112 (26%), Positives = 51/112 (44%), Gaps = 26/112 (23%)
Query: 72 LKTTGCGLPPGPGGSIGALEGVSYLVVV-----------GIVAWSIYTKTKTGSG----- 115
L+ G G+ G GG L+ ++ + + GI+ +S+ T + GSG
Sbjct: 192 LQQFGLGIVIGLGGGYLLLQMINRIALPAGLYPLLALSGGILIFSLTTALE-GSGILAVY 250
Query: 116 -----LPNGP----FGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPA 158
L N P +G+L +GL++LA +A+ +V GL +P +PA
Sbjct: 251 LCGFLLGNRPIRNRYGILQNFDGLAWLAQIAMFLVLGLLVNPSDLLPIAIPA 302
>CRCB_COREF (Q8FMR6) Protein crcB homolog
Length = 138
Score = 29.3 bits (64), Expect = 4.1
Identities = 20/68 (29%), Positives = 30/68 (43%), Gaps = 4/68 (5%)
Query: 78 GLPPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPN----GPFGLLGAVEGLSYL 133
GL G G ++GA ++ + G W I GS L GPF G + G +
Sbjct: 41 GLSVGAGAAVGACARLALTMQFGEGLWPILAINMLGSFLMGRYRPGPFWGTGVLGGFTTF 100
Query: 134 ALVAIVVV 141
+ A+V+V
Sbjct: 101 SAFAVVLV 108
>MGE1_HUMAN (Q9HCI5) Melanoma-associated antigen E1 (MAGE-E1
antigen) (Hepatocellular carcinoma-associated protein 1)
Length = 957
Score = 28.9 bits (63), Expect = 5.4
Identities = 19/54 (35%), Positives = 26/54 (47%), Gaps = 2/54 (3%)
Query: 41 EGSDTNSSPTEIAAIAGGLISTPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVS 94
EG+ T+ PT + ++ TP G S L T G G PG + A EG+S
Sbjct: 170 EGTSTSVPPTAYEGPSTSVVPTPDEGPSTSVLPTPGEG--PGTSVPLAATEGLS 221
>FOCA_HAEIN (P43756) Probable formate transporter (Formate channel)
Length = 284
Score = 28.9 bits (63), Expect = 5.4
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 2/46 (4%)
Query: 102 VAWSIYTKTKTGSGLPNGPFGLLGAVEGLSYLALVAIVVVFGLQYF 147
+A+ YT T+T S P+GL V GL + V +VV+ G + F
Sbjct: 50 LAFVFYTTTQTASA--GAPWGLTKLVGGLVFSLGVIMVVILGSELF 93
>FINC_RAT (P04937) Fibronectin precursor (FN)
Length = 2477
Score = 28.9 bits (63), Expect = 5.4
Identities = 30/95 (31%), Positives = 39/95 (40%), Gaps = 9/95 (9%)
Query: 2 LLFSPISTPKTFTNHLNHPLRKPKSHHVRLKLKITNMAKEG--SDTNSSPTEIAAIAGGL 59
L F+ +S P T T P K + VR +T K G + N SP + I GL
Sbjct: 1818 LKFTQVS-PTTLTAQWTAPSVKLTGYRVR----VTPKEKTGPMKEINLSPDSTSVIVSGL 1872
Query: 60 ISTPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVS 94
+ S+Y LK T P G + LE VS
Sbjct: 1873 MVATKYEVSVYALKDTLTSRP--AQGVVTTLENVS 1905
>CTG1_HUMAN (P78358) Cancer/testis antigen 1B (Autoimmunogenic
cancer/testis antigen NY-ESO-1)
Length = 180
Score = 28.9 bits (63), Expect = 5.4
Identities = 19/56 (33%), Positives = 23/56 (40%), Gaps = 2/56 (3%)
Query: 76 GCGLPPGPGGSIGALEGVSYLVVVGIVAWSIYTKTKTGSGLPNGPFGLLGAVEGLS 131
G G+P GPGG+ G G + G G P GP G GA GL+
Sbjct: 20 GPGIPDGPGGNAGGPGEAGATGGRGPRGAGAARASGPGGGAPRGPHG--GAASGLN 73
>AQL1_THEAQ (P08594) Aqualysin I precursor (EC 3.4.21.-)
Length = 513
Score = 28.9 bits (63), Expect = 5.4
Identities = 16/67 (23%), Positives = 28/67 (40%), Gaps = 3/67 (4%)
Query: 30 RLKLKITNMAKEGSDTNSSPTEIAAIAGGLISTPVIGWSLYTLKTTGCGLPPGPGGSIGA 89
R ++ + G D N T +A GG+ +LY ++ C G G + G
Sbjct: 179 RARVGYDALGGNGQDCNGHGTHVAGTIGGVTYGVAKAVNLYAVRVLDCN---GSGSTSGV 235
Query: 90 LEGVSYL 96
+ GV ++
Sbjct: 236 IAGVDWV 242
>PTCC_ECOLI (P17334) PTS system, N,N'-diacetylchitobiose-specific
IIC component (EIIC-Chb)
(N,N'-diacetylchitobiose-permease IIC component)
(Phosphotransferase enzyme II, C component)
Length = 452
Score = 28.5 bits (62), Expect = 7.0
Identities = 21/71 (29%), Positives = 34/71 (47%), Gaps = 5/71 (7%)
Query: 79 LPPGPGGSIGAL-EGVSYLVVVGIVAWSIYTKTKTGSGLP----NGPFGLLGAVEGLSYL 133
+P S AL G L V+GI+AW++ T + + P LG+V G +Y+
Sbjct: 179 VPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTISTPLASLGSVVGWAYV 238
Query: 134 ALVAIVVVFGL 144
V ++ FG+
Sbjct: 239 IFVPLLWFFGI 249
>PEND_MOUSE (Q9R155) Pendrin (Sodium-independent chloride/iodide
transporter)
Length = 780
Score = 28.1 bits (61), Expect = 9.1
Identities = 11/23 (47%), Positives = 17/23 (73%)
Query: 122 GLLGAVEGLSYLALVAIVVVFGL 144
GL+G ++G++Y L A+ V FGL
Sbjct: 95 GLVGTLQGMAYALLAAVPVQFGL 117
>P121_RAT (P52591) Nuclear envelope pore membrane protein POM 121
(Pore membrane protein of 121 kDa) (P145)
Length = 1199
Score = 28.1 bits (61), Expect = 9.1
Identities = 27/76 (35%), Positives = 33/76 (42%), Gaps = 5/76 (6%)
Query: 53 AAIAGGLISTPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVS-YLV-VVGIVAWSIYTKT 110
AA GG P +G +T GCG P G AL G++ YLV +AW +
Sbjct: 6 AAADGGERRRPPLGVREGRGRTRGCGGPAGAAALGLALLGLALYLVPAAAALAWLAVGAS 65
Query: 111 KTGSGL---PNGPFGL 123
GL P GP GL
Sbjct: 66 AAWWGLSREPRGPRGL 81
>CVRA_SHIFL (Q83RQ1) Cell volume regulation protein A
Length = 578
Score = 28.1 bits (61), Expect = 9.1
Identities = 13/38 (34%), Positives = 23/38 (60%)
Query: 121 FGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPA 158
+G+L +GL++LA +A+ +V GL +P +PA
Sbjct: 265 YGILQNFDGLAWLAQIAMFLVLGLLVNPSDLLPIAIPA 302
>CVRA_ECOL6 (P59239) Cell volume regulation protein A
Length = 578
Score = 28.1 bits (61), Expect = 9.1
Identities = 13/38 (34%), Positives = 23/38 (60%)
Query: 121 FGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPA 158
+G+L +GL++LA +A+ +V GL +P +PA
Sbjct: 265 YGILQNFDGLAWLAQIAMFLVLGLLVNPSDLLPIAIPA 302
>ACSA_METSO (P27095) Acetyl-coenzyme A synthetase (EC 6.2.1.1)
(Acetate--CoA ligase) (Acyl-activating enzyme)
Length = 672
Score = 28.1 bits (61), Expect = 9.1
Identities = 15/45 (33%), Positives = 24/45 (53%)
Query: 47 SSPTEIAAIAGGLISTPVIGWSLYTLKTTGCGLPPGPGGSIGALE 91
+SP I + G + P+ G+ + L G +P G GG+I AL+
Sbjct: 447 NSPLPITPLKPGSCTFPLPGYDISILDEEGNEVPLGSGGNIVALK 491
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.140 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,752,233
Number of Sequences: 164201
Number of extensions: 1072122
Number of successful extensions: 2623
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 2617
Number of HSP's gapped (non-prelim): 19
length of query: 163
length of database: 59,974,054
effective HSP length: 102
effective length of query: 61
effective length of database: 43,225,552
effective search space: 2636758672
effective search space used: 2636758672
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Medicago: description of AC147178.12