BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147012.12 + phase: 0
(750 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DSPP_RAT (Q62598) Dentin sialophosphoprotein precursor [Contains... 35 0.73
GML1_MOUSE (Q920G9) Germ cell-less protein-like 1 (mGcl-1) (DP-i... 35 0.95
POLG_EMCVD (P17594) Genome polyprotein [Contains: Coat protein V... 34 1.6
POLG_EMCVB (P17593) Genome polyprotein [Contains: Coat protein V... 34 1.6
DSPP_HUMAN (Q9NZW4) Dentin sialophosphoprotein precursor [Contai... 34 1.6
ARLY_CORGL (O88101) Argininosuccinate lyase (EC 4.3.2.1) (Argino... 34 1.6
POLG_EMCV (P03304) Genome polyprotein [Contains: Coat protein VP... 33 2.1
GCLL_HUMAN (Q8NEA9) Germ cell-less protein-like 1 like 33 2.1
GLSA_STRCO (P57755) Probable glutaminase (EC 3.5.1.2) 33 2.8
ASPM_PANTR (P62293) Abnormal spindle-like microcephaly-associate... 33 2.8
ASPM_HYLLA (P62290) Abnormal spindle-like microcephaly-associate... 33 2.8
ASPM_HUMAN (Q8IZT6) Abnormal spindle-like microcephaly-associate... 33 2.8
ASPM_GORGO (P62289) Abnormal spindle-like microcephaly-associate... 33 2.8
ARLY_COREF (Q8FTM8) Argininosuccinate lyase (EC 4.3.2.1) (Argino... 33 2.8
SPEN_DROME (Q8SX83) Split ends protein 32 4.7
PCX_DROME (P18490) Pecanex protein 32 4.7
DSPP_MOUSE (P97399) Dentin sialophosphoprotein precursor (Dentin... 32 4.7
SX10_HUMAN (P56693) Transcription factor SOX-10 32 6.2
GML2_MOUSE (Q99N64) Germ cell-less protein-like 2 (mGclh) 32 6.2
FPTA_PSEAE (P42512) Fe(III)-pyochelin receptor precursor 32 6.2
>DSPP_RAT (Q62598) Dentin sialophosphoprotein precursor [Contains:
Dentin phosphoprotein (Dentin phosphophoryn) (DPP);
Dentin sialoprotein (DSP)]
Length = 687
Score = 35.0 bits (79), Expect = 0.73
Identities = 46/177 (25%), Positives = 70/177 (38%), Gaps = 7/177 (3%)
Query: 108 VEHPAHG----IPGSESGTGIHSDNTKGKGIADGCTNKMLETDL-GKMSLKSDLKDTSMP 162
+E+ HG S G SD+ G+ + T+ ++D G +S KD S
Sbjct: 471 IENGNHGDASYTSDESSDNGSDSDSHAGEDDSSDDTSDTDDSDSNGDDDSESKDKDESDN 530
Query: 163 NLLVELGDSEGDFNSDICDSNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRT 222
+ DSE +S DS+ S D S + S SD S + SD
Sbjct: 531 SNHDNDSDSESKSDSSDSDSDSSDSSDSSDSSDSSETSDSSDSSDTSDSSDSSDSSDSSN 590
Query: 223 SCYVEVPLGAGTTTGVGIEGPSEEGSCYHSDNNNRLVRDQSRDSFSSSSCSGLTSSE 279
S + + ++ G +G S + SD++N D S S SSSS S + S+
Sbjct: 591 SS--DTSDSSDSSDGDSSDGDSSDSDSSDSDSSNSSDSDSSDSSDSSSSDSSDSDSD 645
>GML1_MOUSE (Q920G9) Germ cell-less protein-like 1 (mGcl-1)
(DP-interacting protein) (DIP)
Length = 524
Score = 34.7 bits (78), Expect = 0.95
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 4/69 (5%)
Query: 26 PPSHHSDNNDPTTAELRALDC----NLASLCEHVQIEGFNSGSFSDIVVNAMGSTYHLHR 81
P S D+ D + R L+ L S +++ F +G SDI + A+G + LH+
Sbjct: 62 PDSETDDDEDEGDEQQRLLNTPRRKKLKSTSKYIYQTLFLNGENSDIKICALGEEWSLHK 121
Query: 82 LILSRSSYF 90
+ L +S YF
Sbjct: 122 IYLCQSGYF 130
>POLG_EMCVD (P17594) Genome polyprotein [Contains: Coat protein VP4
(Rho); Coat protein VP2 (Beta); Coat protein VP3 (Gamma);
Coat protein VP1 (Alpha); Picornain 2A (EC 3.4.22.29)
(Core protein P2A) (G); Core protein P2B (I); Core
protein P2C (C); Core pr
Length = 2292
Score = 33.9 bits (76), Expect = 1.6
Identities = 26/72 (36%), Positives = 33/72 (45%), Gaps = 3/72 (4%)
Query: 639 PSGCEQISAIVIIVNSLNDNAGLFLHRRKAEITDVHRKVHMYVDSREKVTARYQLTCPSK 698
PSGC S + I+N++ AGL+L + E DV KV Y D V YQL
Sbjct: 2124 PSGCAATSMLNTIMNNIIIRAGLYLTYKNFEFDDV--KVLSYGDDL-LVATNYQLNFDKV 2180
Query: 699 REMLVFGSFKQT 710
R L +K T
Sbjct: 2181 RASLAKTGYKIT 2192
>POLG_EMCVB (P17593) Genome polyprotein [Contains: Coat protein VP4
(Rho); Coat protein VP2 (Beta); Coat protein VP3 (Gamma);
Coat protein VP1 (Alpha); Picornain 2A (EC 3.4.22.29)
(Core protein P2A) (G); Core protein P2B (I); Core
protein P2C (C); Core pr
Length = 2292
Score = 33.9 bits (76), Expect = 1.6
Identities = 26/72 (36%), Positives = 33/72 (45%), Gaps = 3/72 (4%)
Query: 639 PSGCEQISAIVIIVNSLNDNAGLFLHRRKAEITDVHRKVHMYVDSREKVTARYQLTCPSK 698
PSGC S + I+N++ AGL+L + E DV KV Y D V YQL
Sbjct: 2124 PSGCAATSMLNTIMNNIIIRAGLYLTYKNFEFDDV--KVLSYGDDL-LVATNYQLNFDKV 2180
Query: 699 REMLVFGSFKQT 710
R L +K T
Sbjct: 2181 RASLAKTGYKIT 2192
>DSPP_HUMAN (Q9NZW4) Dentin sialophosphoprotein precursor [Contains:
Dentin phosphoprotein (Dentin phosphophoryn) (DPP);
Dentin sialoprotein (DSP)]
Length = 1253
Score = 33.9 bits (76), Expect = 1.6
Identities = 40/161 (24%), Positives = 60/161 (36%), Gaps = 3/161 (1%)
Query: 118 SESGTGIHSDNTKGKGIADGCTNKMLETDLGKMSLKSDLKDTSMPNLLVELGDSEGDFNS 177
S S S N+ + ++ +D S SD D+S + GDS +S
Sbjct: 912 SNSSDSSDSSNSSDSSESSNSSDNSNSSDSSNSSDSSDSSDSSNSSDSSNSGDSSN--SS 969
Query: 178 DICDSNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTSCYVEVPLGAGTTTG 237
D DSN S + S + + S SD S ++ SD S + ++
Sbjct: 970 DSSDSNSSDSSDSSNSSDSSDSSDSSDSSDSSDSSNSSDSSDSSDSSDSSNSSDSSNSSD 1029
Query: 238 VGIEGPSEEGSCYHSDNNNRLVRDQSRDSFSSSSCSGLTSS 278
S + S SD++N S DS SS SG + S
Sbjct: 1030 SSDSSDSSDSS-DSSDSSNSSDSSDSSDSSDSSDSSGSSDS 1069
Score = 33.1 bits (74), Expect = 2.8
Identities = 41/162 (25%), Positives = 60/162 (36%), Gaps = 13/162 (8%)
Query: 118 SESGTGIHSDNTKGKGIADGCTNKMLETDLGKMSLKSDLKDTSMPNLLVELGDSEGDFNS 177
S+S + SD++ +D +D S SD D+S N DS NS
Sbjct: 851 SDSDSSNRSDSSNSSDSSDS-------SDSSNSSDSSDSSDSSDSNESSNSSDSSDSSNS 903
Query: 178 DICDSNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTSCYVEVPLGAGTTTG 237
DS+ S D S + N S S+ S ++ SD S + ++
Sbjct: 904 SDSDSSDSSN--SSDSSDSSNSSDSSESSNSSDNSNSSDSSNSSDSSDSSDSSNSSDSSN 961
Query: 238 VGIEGPSEEGSCYHSDNNNRLVRDQSRDSFSSSSCSGLTSSE 279
G S + SD+N+ D S S SS S SS+
Sbjct: 962 SGDSSNSSDS----SDSNSSDSSDSSNSSDSSDSSDSSDSSD 999
>ARLY_CORGL (O88101) Argininosuccinate lyase (EC 4.3.2.1)
(Arginosuccinase) (ASAL)
Length = 477
Score = 33.9 bits (76), Expect = 1.6
Identities = 20/54 (37%), Positives = 29/54 (53%)
Query: 84 LSRSSYFRFELAFHTFLAKSAHCKVEHPAHGIPGSESGTGIHSDNTKGKGIADG 137
LS S++F + LA + LA AH KV H A + + T + + GK +ADG
Sbjct: 27 LSVSTHFDWVLAPYDVLASKAHAKVLHQADLLSDEDLATMLAGLDQLGKDVADG 80
>POLG_EMCV (P03304) Genome polyprotein [Contains: Coat protein VP4
(Rho); Coat protein VP2 (Beta); Coat protein VP3 (Gamma);
Coat protein VP1 (Alpha); Picornain 2A (EC 3.4.22.29)
(Core protein P2A) (G); Core protein P2B (I); Core
protein P2C (C); Core pro
Length = 2290
Score = 33.5 bits (75), Expect = 2.1
Identities = 26/72 (36%), Positives = 33/72 (45%), Gaps = 3/72 (4%)
Query: 639 PSGCEQISAIVIIVNSLNDNAGLFLHRRKAEITDVHRKVHMYVDSREKVTARYQLTCPSK 698
PSGC S + I+N++ AGL+L + E DV KV Y D V YQL
Sbjct: 2122 PSGCAATSMLNTIMNNIIIRAGLYLTYKNFEFDDV--KVLSYGDDL-LVATNYQLDFDKV 2178
Query: 699 REMLVFGSFKQT 710
R L +K T
Sbjct: 2179 RASLAKTGYKIT 2190
>GCLL_HUMAN (Q8NEA9) Germ cell-less protein-like 1 like
Length = 526
Score = 33.5 bits (75), Expect = 2.1
Identities = 23/86 (26%), Positives = 41/86 (46%), Gaps = 6/86 (6%)
Query: 11 PPSPYNQRSASIPK--QPPSHHSDNNDPTTAELRALDC----NLASLCEHVQIEGFNSGS 64
P S +RS+ + P SH ++ + + L+ L S +++ F +G
Sbjct: 47 PGSHKRKRSSGACRYCDPDSHREEHEEEGDKQQPLLNTPARKKLRSTSKYIYQTLFLNGE 106
Query: 65 FSDIVVNAMGSTYHLHRLILSRSSYF 90
SDI + A+G + LH++ L +S YF
Sbjct: 107 NSDIKICALGEEWRLHKIYLCQSGYF 132
>GLSA_STRCO (P57755) Probable glutaminase (EC 3.5.1.2)
Length = 307
Score = 33.1 bits (74), Expect = 2.8
Identities = 33/109 (30%), Positives = 43/109 (39%), Gaps = 10/109 (9%)
Query: 428 SFRPVRVHVRGAIDGLAGIGRGTTFVPA-AASPPTRF-----VFSRVPFGVGNRNYPQSA 481
+FRPV + I+ G GR ++PA AA P RF +GVG+ P SA
Sbjct: 6 TFRPVLERIAEEIERTPGSGRPADYIPALAARDPRRFGMAVAELDGTVYGVGDWREPFSA 65
Query: 482 ANDDSEARADHNGDLSGDGLTALVGLSQGGNYGTNIHTELTQREQEMGL 530
+ + GD L VG G N L Q E E G+
Sbjct: 66 QSLTKVFTLALDLAREGDALWEHVGREPSG----NPFNSLVQLEYENGI 110
>ASPM_PANTR (P62293) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3477
Score = 33.1 bits (74), Expect = 2.8
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 9/73 (12%)
Query: 182 SNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTS--------CYVEVPLGAG 233
+N+RV +++SP P+ + GP+ S P + S C+ +V LGA
Sbjct: 2 ANRRVGRGCWEVSPTERRP-PAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGAS 60
Query: 234 TTTGVGIEGPSEE 246
T + ++ P+EE
Sbjct: 61 RTLSLALDNPNEE 73
>ASPM_HYLLA (P62290) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3477
Score = 33.1 bits (74), Expect = 2.8
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 9/73 (12%)
Query: 182 SNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTS--------CYVEVPLGAG 233
+N+RV +++SP P+ + GP+ S P + S C+ +V LGA
Sbjct: 2 ANRRVGRGCWEVSPTERRP-PAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGAS 60
Query: 234 TTTGVGIEGPSEE 246
T + ++ P+EE
Sbjct: 61 RTLSLALDNPNEE 73
>ASPM_HUMAN (Q8IZT6) Abnormal spindle-like microcephaly-associated
protein (Abnormal spindle protein homolog) (Asp homolog)
Length = 3477
Score = 33.1 bits (74), Expect = 2.8
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 9/73 (12%)
Query: 182 SNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTS--------CYVEVPLGAG 233
+N+RV +++SP P+ + GP+ S P + S C+ +V LGA
Sbjct: 2 ANRRVGRGCWEVSPTERRP-PAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGAS 60
Query: 234 TTTGVGIEGPSEE 246
T + ++ P+EE
Sbjct: 61 RTLSLALDNPNEE 73
>ASPM_GORGO (P62289) Abnormal spindle-like microcephaly-associated
protein homolog
Length = 3476
Score = 33.1 bits (74), Expect = 2.8
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 9/73 (12%)
Query: 182 SNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTS--------CYVEVPLGAG 233
+N+RV +++SP P+ + GP+ S P + S C+ +V LGA
Sbjct: 2 ANRRVGRGCWEVSPTERRP-PAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGAS 60
Query: 234 TTTGVGIEGPSEE 246
T + ++ P+EE
Sbjct: 61 RTLSLALDNPNEE 73
>ARLY_COREF (Q8FTM8) Argininosuccinate lyase (EC 4.3.2.1)
(Arginosuccinase) (ASAL)
Length = 477
Score = 33.1 bits (74), Expect = 2.8
Identities = 19/54 (35%), Positives = 30/54 (55%)
Query: 84 LSRSSYFRFELAFHTFLAKSAHCKVEHPAHGIPGSESGTGIHSDNTKGKGIADG 137
LS S++F + LA + LA AH KV H A + ++ T + + G+ +ADG
Sbjct: 27 LSVSTHFDWVLAPYDVLASKAHAKVLHSAGLLSDADLDTMLEGLDQLGRDVADG 80
>SPEN_DROME (Q8SX83) Split ends protein
Length = 5560
Score = 32.3 bits (72), Expect = 4.7
Identities = 23/70 (32%), Positives = 33/70 (46%), Gaps = 2/70 (2%)
Query: 213 SLSDPDGVRTSCYVEVPLGAGTTTGVGIEGPSEEGSCYHSDNNNRLVRDQSRDSFSSSSC 272
S S G + Y++ LG G+T G+G G + +C S NN+ L Q S S S+
Sbjct: 1324 SRSSGGGGAGNSYLQQQLGGGSTGGLGCIGAASSSAC--SLNNSSLNASQGMGSCSGSTF 1381
Query: 273 SGLTSSEWGR 282
SS + R
Sbjct: 1382 LPSPSSRYWR 1391
>PCX_DROME (P18490) Pecanex protein
Length = 2483
Score = 32.3 bits (72), Expect = 4.7
Identities = 46/149 (30%), Positives = 63/149 (41%), Gaps = 28/149 (18%)
Query: 407 NMPGG------GGNIYVAESSTGERNG-SFRPVRVHVRGAIDGLAGIGR----GTTFVPA 455
N+PGG GGN YV++S R G + PV+ I GL R G+ + A
Sbjct: 1934 NVPGGPIPGNLGGN-YVSDSLAVVRGGLAVMPVKPTSTTLIAGLLNRERDETSGSGIMGA 1992
Query: 456 AASPPTRFVFSRVPFGVGNRNYPQ--SAANDDSEARADHNGDLSGDGLTALVGLSQGGNY 513
AS PTR S GV +R P+ S+ D E RA + GG
Sbjct: 1993 GASGPTRATSSG---GVRSRR-PEVGSSRGRDHERRAT----------LPIASGGAGGEP 2038
Query: 514 GTNIHTELTQREQEMGLQSTAGGASTGGI 542
G ++ TQ E E +ST +S+G +
Sbjct: 2039 GKDLTAPQTQVEHEPSPRSTKLSSSSGSL 2067
>DSPP_MOUSE (P97399) Dentin sialophosphoprotein precursor (Dentin
matrix protein-3) (DMP-3) [Contains: Dentin
phosphoprotein (Dentin phosphophoryn) (DPP); Dentin
sialoprotein (DSP)]
Length = 934
Score = 32.3 bits (72), Expect = 4.7
Identities = 42/166 (25%), Positives = 66/166 (39%), Gaps = 8/166 (4%)
Query: 118 SESGTGIHSDNTKGKGIADGCTNKMLETDLGKMSLKSDLKDTSMPNLLVELGDSEGDFNS 177
S +G SD++ G G +D + E++ S SD ++S + DS D +S
Sbjct: 498 SHAGEDDSSDDSSGDGDSDSNGDGDSESEDKDESDSSDHDNSSDSESKSDSSDSSDD-SS 556
Query: 178 DICDSNQRVQLASYDISPNLNPSYPSDMEGPSLGNSLSDPDGVRTSCYVEVPLGAGTTTG 237
D DS+ S D S + + S SD + + SD G S + ++
Sbjct: 557 DSSDSSDSSD--SSDSSDSSDSSDSSDSSDSNSSSDSSDSSGSSDSSDSSDTCDSSDSSD 614
Query: 238 VGIEGPSEEG-----SCYHSDNNNRLVRDQSRDSFSSSSCSGLTSS 278
S + S SD+++ S DS SSSCS + S
Sbjct: 615 SSDSSDSSDSSDSSDSSDSSDSSDSSDSSSSSDSSDSSSCSDSSDS 660
>SX10_HUMAN (P56693) Transcription factor SOX-10
Length = 466
Score = 32.0 bits (71), Expect = 6.2
Identities = 35/143 (24%), Positives = 59/143 (40%), Gaps = 14/143 (9%)
Query: 102 KSAHCKVEHPAHGIPGSESGTGIHSDNTKGKGIADGCTNKMLETDLGKMSLKSD---LKD 158
KSAH HP G P S+ S + G T E GK K D + +
Sbjct: 208 KSAHLDHRHPGEGSPMSDGNPEHPSGQSHGPPTPP--TTPKTELQSGKADPKRDGRSMGE 265
Query: 159 TSMPNLLVELGDSE-GDFNSDICDSNQRVQLASYD--ISPNLNPSYPSDME--GPSLGNS 213
P+ ++ G+ + G+ + ++ + + +A D + PN +P + S G LG++
Sbjct: 266 GGKPH--IDFGNVDIGEISHEVMSNMETFDVAELDQYLPPNGHPGHVSSYSAAGYGLGSA 323
Query: 214 LSDPDGVRTSCYVEVPLGAGTTT 236
L+ G S ++ P G T
Sbjct: 324 LAVASG--HSAWISKPPGVALPT 344
>GML2_MOUSE (Q99N64) Germ cell-less protein-like 2 (mGclh)
Length = 498
Score = 32.0 bits (71), Expect = 6.2
Identities = 14/31 (45%), Positives = 20/31 (64%)
Query: 60 FNSGSFSDIVVNAMGSTYHLHRLILSRSSYF 90
F G SDI + A+G T+ LH++ L +S YF
Sbjct: 84 FLDGHDSDIKIRALGRTWCLHKVFLCQSGYF 114
>FPTA_PSEAE (P42512) Fe(III)-pyochelin receptor precursor
Length = 720
Score = 32.0 bits (71), Expect = 6.2
Identities = 43/145 (29%), Positives = 61/145 (41%), Gaps = 30/145 (20%)
Query: 210 LGNSLSDPDGVRTSCYVEVPLGA-GTTTGVGIEGPSEEGSCYHSDNNNRLVRDQSRDSFS 268
LGN+ S P + VE+ G+ G G G + LVR + + F+
Sbjct: 140 LGNTASSPQDMAIYERVEILRGSNGLLHGTGNPAATVN-----------LVRKRPQREFA 188
Query: 269 SSSCSGLTSSEWGRY------GTPLLSWGGHVGRRQVKAHPRGNYRDEDDVFINIFEGGS 322
+S+ L++ W RY G P LS G+V R V A Y D D + +G
Sbjct: 189 ASTT--LSAGRWDRYRAEVDVGGP-LSASGNVRGRAVAA-----YEDRDYFYDVADQGTR 240
Query: 323 LLY----CNMSFDALLNVRKQLEEI 343
LLY ++S D LL V Q + I
Sbjct: 241 LLYGVTEFDLSPDTLLTVGAQYQHI 265
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.134 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,770,990
Number of Sequences: 164201
Number of extensions: 4351188
Number of successful extensions: 11304
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 11275
Number of HSP's gapped (non-prelim): 53
length of query: 750
length of database: 59,974,054
effective HSP length: 118
effective length of query: 632
effective length of database: 40,598,336
effective search space: 25658148352
effective search space used: 25658148352
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 70 (31.6 bits)
Medicago: description of AC147012.12