BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147006.6 + phase: 0 /pseudo
         (797 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog               44  0.001
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ...    36  0.46
POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains...    34  1.7
M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310...    33  3.9
LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog               32  6.6

>LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog
          Length = 1259

 Score = 44.3 bits (103), Expect = 0.001
 Identities = 38/181 (20%), Positives = 80/181 (43%), Gaps = 30/181 (16%)

Query: 29  RVELLVEDIKMLDEKGEEGTLTE*EVGLHKGKLVELWRLLKAKDTLIT----QRSRSRWL 84
           +++ L+  +K L+++ +  +         + +++++   LK  +T  T      SRS + 
Sbjct: 312 KIDTLISQLKELEKQEQTNSKAS-----RRQEIIKIRAELKEIETQKTLQKINESRSWFF 366

Query: 85  KKGDANSKFFHKCIKLRKSINSIKALEENEGWVVS-PFEVCRKVVNYFTNHFAE------ 137
           +K +   +   + IK ++  N I  ++ + G + + P E+   +  Y+ + +A       
Sbjct: 367 EKINKIDRPLARLIKKKREKNQIDTIKNDRGDITTDPTEIQTTIREYYKHLYANKLENLE 426

Query: 138 ------DRWDRPRLDRVDFESLTEVENGLLVAPFSLLEIEEVVRDSDGGKSPGPNGYNFS 191
                 D +  PRL++ + ESL          P +  EIE ++      KSPGP G+   
Sbjct: 427 EMDKFLDTYTLPRLNQEEVESLNR--------PITSSEIEAIINSLPNKKSPGPEGFTAE 478

Query: 192 F 192
           F
Sbjct: 479 F 479


>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein
           (ORF 2)
          Length = 1308

 Score = 35.8 bits (81), Expect = 0.46
 Identities = 42/187 (22%), Positives = 79/187 (41%), Gaps = 8/187 (4%)

Query: 11  LKVILKE*IKKEYGGMEDRVELLVEDIKMLDEK--GEEGTLTE*EVGLHKGKLVELWRLL 68
           LK++ +E  K   G     +E L  ++  L+++  G E    + E  L + + +      
Sbjct: 293 LKLLCQEYTKSVSGQRNAEIEALNGEVLDLEQRLSGSEDQALQCEY-LERKEALRNMEQR 351

Query: 69  KAKDTLITQRSRSRWLKKGDANSKFFHKCIKLRKSINSIKALEENEGWVVSPFEVCR-KV 127
           +A+   +  RSR + L   D  S+FF+   K + +   I  L   +G  +   E  R + 
Sbjct: 352 QARGAFV--RSRMQLLCDMDRGSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDRA 409

Query: 128 VNYFTNHFAEDRWDRPRLDRV--DFESLTEVENGLLVAPFSLLEIEEVVRDSDGGKSPGP 185
            +++ N F+ D       + +      ++E     L  P +L E+ + +R     KSPG 
Sbjct: 410 RSFYQNLFSPDPISPDACEELWDGLPVVSERRKERLETPITLDELSQALRLMPHNKSPGL 469

Query: 186 NGYNFSF 192
           +G    F
Sbjct: 470 DGLTIEF 476


>POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains:
           Reverse transcriptase (EC 2.7.7.49); Endonuclease]
          Length = 1300

 Score = 33.9 bits (76), Expect = 1.7
 Identities = 32/142 (22%), Positives = 56/142 (38%), Gaps = 17/142 (11%)

Query: 67  LLKAKDTLITQRSRSRWLKKGDANSKFFHKCIKLRKSINSI-----------KALEENEG 115
           ++K +  +    +R    +     S FF K  K+ K +  +           K   E   
Sbjct: 366 IIKLRGEINQVETRRTIQRINQTRSWFFEKINKIDKPLARLTKGHRDKILINKIRNEKGD 425

Query: 116 WVVSPFEVCRKVVNYFTNHFAE-----DRWDRPRLDRVDFESLTEVENGLLVAPFSLLEI 170
               P E+   + +++   ++      D  D+  LDR     L + +   L +P S  EI
Sbjct: 426 ITTDPEEIQNTIRSFYKRLYSTKLENLDEMDK-FLDRYQVPKLNQDQVDHLNSPISPKEI 484

Query: 171 EEVVRDSDGGKSPGPNGYNFSF 192
           E V+      KSPGP+G++  F
Sbjct: 485 EAVINSLPTKKSPGPDGFSAEF 506


>M310_ARATH (P93295) Hypothetical mitochondrial protein AtMg00310
           (ORF154)
          Length = 154

 Score = 32.7 bits (73), Expect = 3.9
 Identities = 14/36 (38%), Positives = 22/36 (60%), Gaps = 1/36 (2%)

Query: 549 KFYGEEWEGVKKISWVKWELVCQNKR-NGSLGVNSL 583
           +F+    E  +KISWV W+ +C++K  +G LG   L
Sbjct: 27  EFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDL 62


>LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog
          Length = 1260

 Score = 32.0 bits (71), Expect = 6.6
 Identities = 23/99 (23%), Positives = 47/99 (47%), Gaps = 8/99 (8%)

Query: 99  KLRKSINSIKALEENEGWVVSPFEVCRKVVNYFTNHFAEDRWDRPR-----LDRVDFESL 153
           +++  I+SI+    N+     P E+ +K++N +       +++  +     L+      L
Sbjct: 384 RVKSLISSIR--NGNDEITTDPSEI-QKILNEYYKKLYSHKYENLKEIDQYLEACHLPRL 440

Query: 154 TEVENGLLVAPFSLLEIEEVVRDSDGGKSPGPNGYNFSF 192
           ++ E  +L  P S  EI   +++    KSPGP+G+   F
Sbjct: 441 SQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEF 479


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.356    0.160    0.625 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,954,078
Number of Sequences: 164201
Number of extensions: 3251514
Number of successful extensions: 14212
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 14211
Number of HSP's gapped (non-prelim): 5
length of query: 797
length of database: 59,974,054
effective HSP length: 118
effective length of query: 679
effective length of database: 40,598,336
effective search space: 27566270144
effective search space used: 27566270144
T: 11
A: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.7 bits)
S2: 70 (31.6 bits)