BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC147000.5 - phase: 0
(221 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
H11_GLYSA (P40266) Histone H1-I 38 0.016
GARP_PLAFF (P13816) Glutamic acid-rich protein precursor 35 0.11
KINH_STRPU (P35978) Kinesin heavy chain 35 0.14
MRAW_LACLA (Q9CH73) S-adenosyl-methyltransferase mraW (EC 2.1.1.-) 35 0.18
TOP1_DROME (P30189) DNA topoisomerase I (EC 5.99.1.2) 33 0.40
ANRY_HUMAN (Q6UB99) Ankyrin repeat domain protein 11 (Ankyrin re... 33 0.40
TRT3_RABIT (P02641) Troponin T, fast skeletal muscle isoforms 33 0.53
H11_GLYBA (P40263) Histone H1-I 33 0.53
CLPB_PORGI (Q7MVE7) Chaperone clpB 33 0.69
YO33_BPHP1 (P51737) Hypothetical 28.3 kDa protein in LYS 3'regio... 31 2.0
RLA2_DROME (P05389) 60S acidic ribosomal protein P2 (Acidic ribo... 31 2.0
INCE_HUMAN (Q9NQS7) Inner centromere protein 31 2.0
CLPB_CLOTE (Q898C7) Chaperone clpB 31 2.0
TRT3_MOUSE (Q9QZ47) Troponin T, fast skeletal muscle isoforms 31 2.6
TRT3_HUMAN (P45378) Troponin T, fast skeletal muscle isoforms (B... 31 2.6
TNP6_ENTFC (Q06238) Transposase for transposon Tn1546 31 2.6
TMPB_TREPH (P29720) Treponemal membrane protein B precursor (Ant... 31 2.6
RFC1_SCHPO (O60182) Probable activator 1 subunit 1 (Replication ... 31 2.6
H1B_XENLA (P06893) Histone H1B 31 2.6
GOB1_HUMAN (Q14789) Golgi autoantigen, golgin subfamily B member... 31 2.6
>H11_GLYSA (P40266) Histone H1-I
Length = 233
Score = 38.1 bits (87), Expect = 0.016
Identities = 30/96 (31%), Positives = 44/96 (45%), Gaps = 4/96 (4%)
Query: 123 DEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAA 182
D+P+ K+ P +I++K KK + AV+ AA A KK++A+ AK AA
Sbjct: 131 DKPEAAPKKKAPRPKREIEKKEKKVVAKKPKPAVEKK----AAKPAAKKAVAKPAAKKAA 186
Query: 183 AKAKAKREEERIEKLKKIRGERWLPSMAKEMQAKIK 218
AK AK + K + P+ K AK K
Sbjct: 187 AKPAAKEPAAKASPKKAAAKPKAKPTPKKSTAAKPK 222
>GARP_PLAFF (P13816) Glutamic acid-rich protein precursor
Length = 678
Score = 35.4 bits (80), Expect = 0.11
Identities = 33/162 (20%), Positives = 65/162 (39%), Gaps = 27/162 (16%)
Query: 54 KKHNDNQNPKSPKIKHYQLKALKLLDDILENTIEIVKEPIPVLDEDPNVDDCGIRLFR-H 112
K K K KH + K K+ +++N IE DED + G+ +
Sbjct: 415 KSKGKKDKGKKDKGKHKKAKKEKVKKHVVKNVIE---------DEDKD----GVEIINLE 461
Query: 113 SKPGIVFDHADEPQPPMKRPK-----------LVPGEDIDEKSKKFRRRIRSIAVDGNDL 161
K H P+ +P+ L+ ++EK+ + ++ I G
Sbjct: 462 DKEACEEQHITVESRPLSQPQCKLIDEPEQLTLMDKSKVEEKNLSIQEQL--IGTIGRVN 519
Query: 162 IAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKLKKIRGE 203
+ D +KK +A++E + + +EE++ E+ K+++ E
Sbjct: 520 VVPRRDNHKKKMAKIEEAELQKQKHVDKEEDKKEESKEVQEE 561
Score = 29.6 bits (65), Expect = 5.8
Identities = 16/49 (32%), Positives = 27/49 (54%)
Query: 170 KKSLARLEAKDAAAKAKAKREEERIEKLKKIRGERWLPSMAKEMQAKIK 218
++ L L+ K+ K K +E+E+IEK KK + E+ KE + + K
Sbjct: 254 EEMLKTLDKKERKQKEKEMKEQEKIEKKKKKQEEKEKKKQEKERKKQEK 302
>KINH_STRPU (P35978) Kinesin heavy chain
Length = 1031
Score = 35.0 bits (79), Expect = 0.14
Identities = 37/144 (25%), Positives = 72/144 (49%), Gaps = 18/144 (12%)
Query: 66 KIKHYQLKALKLLDDILENTIEIVK--EPIPVLDEDPNVDDCGIRLFRHSKPGIVFDH-A 122
K K+ +LKA L ILEN ++ + E +PV ++ D+ L KP + H +
Sbjct: 348 KEKNGRLKAQLL---ILENELQRWRAGESVPVKEQGNKNDEI---LKEMMKPKQMTVHVS 401
Query: 123 DEPQPPMKRPKLVPGEDIDEKSK------KFRRRIRSIAVDGNDLIAAANDAYK---KSL 173
+E + + K+ E +DEK + +++ ++ +L+++ Y+ +
Sbjct: 402 EEEKNKWEEEKVKLYEQLDEKDSEIDNQSRLTEKLKQQMLEQEELLSSMQRDYELLQSQM 461
Query: 174 ARLEAKDAAAKAKAKREEERIEKL 197
RLEA++AAAK +AK + +E++
Sbjct: 462 GRLEAENAAAKEEAKEVLQALEEM 485
>MRAW_LACLA (Q9CH73) S-adenosyl-methyltransferase mraW (EC 2.1.1.-)
Length = 317
Score = 34.7 bits (78), Expect = 0.18
Identities = 19/90 (21%), Positives = 47/90 (52%), Gaps = 5/90 (5%)
Query: 135 VPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERI 194
+P +++ +K +R ++I ++ ND + AA ++ ++++ L+ + E+R+
Sbjct: 196 LPQKELKKKGHPAKRIFQAIRIEVNDELGAAEESIEQAIDLLKVDGRISVITFHSLEDRL 255
Query: 195 EKL-----KKIRGERWLPSMAKEMQAKIKI 219
K + + LP + K+M+AK+K+
Sbjct: 256 TKTIFKEYSTVNVPKGLPMLPKDMEAKLKL 285
>TOP1_DROME (P30189) DNA topoisomerase I (EC 5.99.1.2)
Length = 972
Score = 33.5 bits (75), Expect = 0.40
Identities = 27/97 (27%), Positives = 43/97 (43%), Gaps = 5/97 (5%)
Query: 114 KPGIVFDHADEPQPPMKRPKLVPGEDIDEKSK-KFRRRIRSIAVDGNDLIAAANDAYKKS 172
+P D+ D+ P+ K V E I ++SK K ++R++ D + KK
Sbjct: 344 RPDGGMDNDDDDDIPLLARKKVKKEKIKKESKEKSKKRVKEEPSDDYGNVKPKKKKMKKE 403
Query: 173 LARLEAKDAAAKAKAKREEERI----EKLKKIRGERW 205
+ KAKAK EEE + E+ K+ G +W
Sbjct: 404 PEPAVSPGKRQKAKAKVEEEEVWRWWEEEKRADGVKW 440
>ANRY_HUMAN (Q6UB99) Ankyrin repeat domain protein 11 (Ankyrin
repeat-containing cofactor-1)
Length = 2664
Score = 33.5 bits (75), Expect = 0.40
Identities = 45/201 (22%), Positives = 79/201 (38%), Gaps = 45/201 (22%)
Query: 53 KKKHNDNQNPKSPKI----KHYQLKALKLLDDILENTIEIVKEPIPVLDEDPNVDDCGIR 108
KK ND++ S KI K Y A+ +L + + K +++ + D
Sbjct: 1428 KKDKNDSEREPSKKIEKELKPYGSSAINILKEKKKREKHREKWRD---EKERHRDRHADG 1484
Query: 109 LFRHSKPGIVFDHADEPQPPMKRPKLVPGEDIDEKSK---------KFRRRIRSIAV--- 156
L RH + ++ H DE Q P R K P + +KS+ K + + + A
Sbjct: 1485 LLRHHRDELLRHHRDE-QKPATRDKDSPPRVLKDKSRDEGPRLGDAKLKEKFKDGAEKEK 1543
Query: 157 -------DGNDLIAAANDAYKKSLA------------------RLEAKDAAAKAKAKREE 191
+GND +A + D KK L KD + + KR +
Sbjct: 1544 GDPVKMSNGNDKVAPSKDPGKKDARPREKLLGDGDLMMTSFERMLSQKDLEIEERHKRHK 1603
Query: 192 ERIEKLKKIRGERWLPSMAKE 212
ER+++++K+R P + ++
Sbjct: 1604 ERMKQMEKLRHRSGDPKLKEK 1624
>TRT3_RABIT (P02641) Troponin T, fast skeletal muscle isoforms
Length = 278
Score = 33.1 bits (74), Expect = 0.53
Identities = 28/114 (24%), Positives = 55/114 (47%), Gaps = 7/114 (6%)
Query: 89 VKEPIPVLDEDPNVDDCGIRLFRHSKPGIVFDHADEPQPPMKRPKLVPGEDIDEKSKKFR 148
V EP P + V + + R + + ++P+P + PK+ GE +D + +
Sbjct: 28 VHEPAPEVHVPEEVHEDALEDMREEE-----EEEEKPRPKLTAPKIPEGEKVDFDDIQKK 82
Query: 149 RRIRSIAVDGNDLIAAANDAYKKSLARLEA-KDAAAKAKAKREEERIEKLKKIR 201
R+ + + ++ LI + +A KK L A K+ K +A+R E++ + +K R
Sbjct: 83 RQNKDL-MELQALIDSHFEARKKEEEELVALKERIEKRRAERAEQQRIRAEKER 135
>H11_GLYBA (P40263) Histone H1-I
Length = 233
Score = 33.1 bits (74), Expect = 0.53
Identities = 28/96 (29%), Positives = 40/96 (41%), Gaps = 4/96 (4%)
Query: 123 DEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAA 182
D P+ K+ ++ K KK + AV+ AA A KK+ A+ AK AA
Sbjct: 131 DRPESAPKKKATKTKTRVERKEKKVVAKKPKPAVEKK----AAKPAAKKAAAKPAAKKAA 186
Query: 183 AKAKAKREEERIEKLKKIRGERWLPSMAKEMQAKIK 218
AK AK+ + K + P+ K AK K
Sbjct: 187 AKPAAKKPAAKASPKKAAAKPKAKPTPKKSTPAKPK 222
>CLPB_PORGI (Q7MVE7) Chaperone clpB
Length = 863
Score = 32.7 bits (73), Expect = 0.69
Identities = 20/69 (28%), Positives = 42/69 (59%), Gaps = 10/69 (14%)
Query: 138 EDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYK-----KSLARLEAKDAAAKAKAKREEE 192
E++DE S RRI+ + ++ + I ND K + +A L+ K+A+ KA+ + E++
Sbjct: 408 EELDEIS----RRIKQLEIE-REAIKRENDEEKVQFLDREIAELKEKEASEKAQWQNEKD 462
Query: 193 RIEKLKKIR 201
RI ++++++
Sbjct: 463 RINQIQQLK 471
>YO33_BPHP1 (P51737) Hypothetical 28.3 kDa protein in LYS 3'region
(ORF33)
Length = 258
Score = 31.2 bits (69), Expect = 2.0
Identities = 44/185 (23%), Positives = 73/185 (38%), Gaps = 33/185 (17%)
Query: 29 SVAGTSSYVTSFMNGFSATNNNDTKKKHNDNQNPKSPKIKHYQLKALKLLDDILENTIEI 88
SV+GT V ++ +G K+K N +P++ + A KL+D N +
Sbjct: 34 SVSGTKLGVHNWSHGI--------KEKSNHYLSPENA----VKALAAKLVDYADPNRPKG 81
Query: 89 VKEPIPVLDEDPNVDD--CGIRLFRHSKPGIVF----DHADEPQ----------PPMKRP 132
V++ + ++ N+D + R P F D+A + P M P
Sbjct: 82 VQDVVVIMVTSSNIDQFIAELEKVRELLPEPTFKQALDYAKSSKDLQETKMIKTPTMASP 141
Query: 133 KLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEE 192
DI S + + I A AA + K +A +EA AA K + K E
Sbjct: 142 SFSNSADITPGSARTMQSILRNATS-----AAVAEQTKDPMAMIEALKAAKKERDKANNE 196
Query: 193 RIEKL 197
++EK+
Sbjct: 197 KVEKM 201
>RLA2_DROME (P05389) 60S acidic ribosomal protein P2 (Acidic
ribosomal protein RPA1)
Length = 113
Score = 31.2 bits (69), Expect = 2.0
Identities = 22/63 (34%), Positives = 35/63 (54%), Gaps = 3/63 (4%)
Query: 133 KLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEE 192
K + G+ ID+ K+ R ++ S+ V G +AAA DA + A + K+ AK + K+EE
Sbjct: 44 KELAGKSIDDLIKEGREKLSSMPVGGGGAVAAA-DAAPAAAAGGDKKE--AKKEEKKEES 100
Query: 193 RIE 195
E
Sbjct: 101 ESE 103
>INCE_HUMAN (Q9NQS7) Inner centromere protein
Length = 919
Score = 31.2 bits (69), Expect = 2.0
Identities = 18/74 (24%), Positives = 41/74 (55%), Gaps = 8/74 (10%)
Query: 130 KRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKR 189
K P+L+P + +K+++ +RRI + + D I ++ L+R +++ + ++ R
Sbjct: 51 KEPELMP-KTPSQKNRRKKRRISYVQDENRDPI-------RRRLSRRKSRSSQLSSRRLR 102
Query: 190 EEERIEKLKKIRGE 203
++ +EKL + GE
Sbjct: 103 SKDSVEKLATVVGE 116
>CLPB_CLOTE (Q898C7) Chaperone clpB
Length = 865
Score = 31.2 bits (69), Expect = 2.0
Identities = 26/78 (33%), Positives = 37/78 (47%), Gaps = 11/78 (14%)
Query: 140 IDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEA-KDAAAKAKAKREEERIEKLK 198
IDE R I S+ + D+ K+ + ++E K+A AK K R +ER+E L+
Sbjct: 398 IDEAGAMIRTEIDSLPTE--------LDSIKRKIFQMEIEKEALAKEKDSRSKERLEDLE 449
Query: 199 KIRGERWLPSMAKEMQAK 216
K L KEM AK
Sbjct: 450 KELSN--LKEKDKEMTAK 465
>TRT3_MOUSE (Q9QZ47) Troponin T, fast skeletal muscle isoforms
Length = 271
Score = 30.8 bits (68), Expect = 2.6
Identities = 22/83 (26%), Positives = 45/83 (53%), Gaps = 2/83 (2%)
Query: 120 DHADEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEA- 178
+ ++P+P + PK+ GE +D + +R+ + + ++ LI + +A KK L A
Sbjct: 47 EEEEKPRPKLTAPKIPEGEKVDFDDIQKKRQNKDL-MELQALIDSHFEARKKEEEELIAL 105
Query: 179 KDAAAKAKAKREEERIEKLKKIR 201
K+ K +A+R E++ + +K R
Sbjct: 106 KERIEKRRAERAEQQRIRAEKER 128
>TRT3_HUMAN (P45378) Troponin T, fast skeletal muscle isoforms (Beta
TnTF)
Length = 268
Score = 30.8 bits (68), Expect = 2.6
Identities = 22/80 (27%), Positives = 44/80 (54%), Gaps = 2/80 (2%)
Query: 123 DEPQPPMKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEA-KDA 181
++P+P + PK+ GE +D + +R+ + + ++ LI + +A KK L A K+
Sbjct: 47 EKPRPKLTAPKIPEGEKVDFDDIQKKRQNKDL-MELQALIDSHFEARKKEEEELVALKER 105
Query: 182 AAKAKAKREEERIEKLKKIR 201
K +A+R E++ + +K R
Sbjct: 106 IEKRRAERAEQQRIRAEKER 125
>TNP6_ENTFC (Q06238) Transposase for transposon Tn1546
Length = 988
Score = 30.8 bits (68), Expect = 2.6
Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Query: 140 IDEKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAK 186
I E++ F R++ +A + N L + + K SLARLE KD +AK
Sbjct: 521 ITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLE-KDVPEEAK 566
>TMPB_TREPH (P29720) Treponemal membrane protein B precursor
(Antigen tmpB)
Length = 384
Score = 30.8 bits (68), Expect = 2.6
Identities = 21/58 (36%), Positives = 32/58 (54%), Gaps = 3/58 (5%)
Query: 163 AAANDAYKKSLARLEAKDAAAKAKAKREEER---IEKLKKIRGERWLPSMAKEMQAKI 217
AAA A ++ AR+ A++ AA+ A+ E R E L +GE+ LPS K + K+
Sbjct: 254 AAARKAAEEEAARIAAEEEAARKAAEEEAARKAAEEALYNEKGEKVLPSEYKVLTWKL 311
Score = 30.8 bits (68), Expect = 2.6
Identities = 25/77 (32%), Positives = 34/77 (43%), Gaps = 2/77 (2%)
Query: 142 EKSKKFRRRIRSIAVDGNDLIAAANDAYKKSLARLEAKDAAAKAKAKREEERIEKLKKIR 201
E K + IA D AA A +K+ AKD AAK KA +E+ +K K +
Sbjct: 137 ESLKTVEPEDKVIAKAAADKAAAEKAAKEKAAREKSAKDKAAKEKAAKEKAAKDKAAKEK 196
Query: 202 GERWLPSMAKEMQAKIK 218
+ AK+ AK K
Sbjct: 197 AAK--EKAAKDKAAKEK 211
>RFC1_SCHPO (O60182) Probable activator 1 subunit 1 (Replication
factor C subunit 1) (Replication factor C1)
Length = 934
Score = 30.8 bits (68), Expect = 2.6
Identities = 34/123 (27%), Positives = 50/123 (40%), Gaps = 11/123 (8%)
Query: 33 TSSYVTSFMNGFSATNNNDTKKKHNDNQNPKSPKIKHYQLKALKLLDDILENTIEIVKEP 92
++S + SF G +A +KK + P SPK K K +L D + TIE K P
Sbjct: 2 SNSDIRSFFGGGNA------QKKPKVSPTPTSPKPKRSLKKKRIVLSDDEDGTIENSKVP 55
Query: 93 IPVLDEDPNVDDCGIRLFRHSKPGIVFDHADEPQPPMKRPKLVPGEDIDEKSKKFRRRIR 152
+ I HS P IV H D+ P E +++S + R + R
Sbjct: 56 ASKSKVQKRNESEDI---SHSLPSIV--HEDDKLVGSDGVSTTPDEYFEQQSTRSRSKPR 110
Query: 153 SIA 155
I+
Sbjct: 111 IIS 113
>H1B_XENLA (P06893) Histone H1B
Length = 219
Score = 30.8 bits (68), Expect = 2.6
Identities = 23/105 (21%), Positives = 46/105 (42%), Gaps = 15/105 (14%)
Query: 129 MKRPKLVPGEDIDEKSKKFRRRIRSIAVDGNDLI---AAANDAYKKSLARLEAKDAAAKA 185
+K+ G D+D+ + + + ++++ G + A+ ++K + +LE KD AAK
Sbjct: 65 LKKALAAGGYDVDKNNSRLKLALKALVTKGTLTQVKGSGASGSFKLNKKQLETKDKAAKK 124
Query: 186 K------------AKREEERIEKLKKIRGERWLPSMAKEMQAKIK 218
K AK+ + +K KK+ P K++ K
Sbjct: 125 KPAAPKAKKTAAGAKKAPKSPKKPKKVSAAAKSPKKVKKLAKAAK 169
>GOB1_HUMAN (Q14789) Golgi autoantigen, golgin subfamily B member 1
(Giantin) (Macrogolgin) (Golgi complex-associated
protein, 372-kDa) (GCP372)
Length = 3259
Score = 30.8 bits (68), Expect = 2.6
Identities = 26/67 (38%), Positives = 36/67 (52%), Gaps = 5/67 (7%)
Query: 159 NDLIAAANDAYKKSLARLEA-KDAAAKAKAKREEE-RIEKLKKIRGERWLP---SMAKEM 213
N+L+ + KK ++ LE K K K K E E R E L+KI+G + P S AKE+
Sbjct: 1951 NELLESEMKNLKKCVSELEEEKQQLVKEKTKVESEIRKEYLEKIQGAQKEPGNKSHAKEL 2010
Query: 214 QAKIKIK 220
Q +K K
Sbjct: 2011 QELLKEK 2017
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.311 0.131 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 27,404,468
Number of Sequences: 164201
Number of extensions: 1204072
Number of successful extensions: 4202
Number of sequences better than 10.0: 54
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 46
Number of HSP's that attempted gapping in prelim test: 4161
Number of HSP's gapped (non-prelim): 71
length of query: 221
length of database: 59,974,054
effective HSP length: 106
effective length of query: 115
effective length of database: 42,568,748
effective search space: 4895406020
effective search space used: 4895406020
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 63 (28.9 bits)
Medicago: description of AC147000.5