Medicago
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC147000.15 - phase: 0 /pseudo
         (485 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran...    46  2e-04
M820_ARATH (P92520) Hypothetical mitochondrial protein AtMg00820...    42  0.003
COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contain...    32  4.8

>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
           transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
           Reverse transcriptase (EC 2.7.7.49); Endonuclease]
          Length = 1328

 Score = 46.2 bits (108), Expect = 2e-04
 Identities = 29/87 (33%), Positives = 47/87 (53%), Gaps = 8/87 (9%)

Query: 253 DGDEPVN*DGFVNASKSREWIASMKEELSSLHKNGTWLLVNKPKRMKNMSCI*LFKLKEG 312
           D  EP +    ++  +  + + +M+EE+ SL KNGT+ LV  PK  + + C  +FKLK+ 
Sbjct: 807 DDREPESLKEVLSHPEKNQLMKAMQEEMESLQKNGTYKLVELPKGKRPLKCKWVFKLKKD 866

Query: 313 -----VNYMD*NSRFKARGFT*REGID 334
                V Y    +R   +GF  ++GID
Sbjct: 867 GDCKLVRY---KARLVVKGFEQKKGID 890



 Score = 35.8 bits (81), Expect = 0.25
 Identities = 19/56 (33%), Positives = 28/56 (49%), Gaps = 1/56 (1%)

Query: 1   FNQICKDSGIVRHKQIRGTPRENCLDH*RMDRKIMERFICLMSNANNSTLFWDETV 56
           F + C   GI   K + GTP+ N +   RM+R I+E+   ++  A     FW E V
Sbjct: 560 FEEYCSSHGIRHEKTVPGTPQHNGVAE-RMNRTIVEKVRSMLRMAKLPKSFWGEAV 614



 Score = 32.7 bits (73), Expect = 2.2
 Identities = 20/48 (41%), Positives = 27/48 (55%)

Query: 417  MILKIDDMLIACNIKEEFDKVKVML*IEFQMKDMRAAS*ILAKKILRD 464
            ++L +DDMLI    K    K+K  L   F MKD+  A  IL  KI+R+
Sbjct: 1005 LLLYVDDMLIVGKDKGLIAKLKGDLSKSFDMKDLGPAQQILGMKIVRE 1052


>M820_ARATH (P92520) Hypothetical mitochondrial protein AtMg00820
           (ORF170)
          Length = 170

 Score = 42.4 bits (98), Expect = 0.003
 Identities = 28/76 (36%), Positives = 38/76 (49%), Gaps = 2/76 (2%)

Query: 266 ASKSREWIASMKEELSSLHKNGTWLLVNKPKRMKNMSCI*LFKLK-EGVNYMD-*NSRFK 323
           A K   W  +M+EEL +L +N TW+LV  P     + C  +FK K      +D   +R  
Sbjct: 34  ALKDPGWCQAMQEELDALSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLV 93

Query: 324 ARGFT*REGID*CENY 339
           A+GF   EGI   E Y
Sbjct: 94  AKGFHQEEGIYFVETY 109


>COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contains:
           Copia VLP protein; Copia protease (EC 3.4.23.-)]
          Length = 1409

 Score = 31.6 bits (70), Expect = 4.8
 Identities = 29/116 (25%), Positives = 49/116 (42%), Gaps = 7/116 (6%)

Query: 221 KLRTSTSLSLSSNTTLEN*L**KK*VVVSGEIDGDEPVN*DGFVNASKSREWIASMKEEL 280
           +L+T   +S +      N     K V+ +  I  D P + D          W  ++  EL
Sbjct: 860 RLKTKPQISYNEEDNSLN-----KVVLNAHTIFNDVPNSFDEIQYRDDKSSWEEAINTEL 914

Query: 281 SSLHKNGTWLLVNKPKRMKNMSCI*LFKLK--EGVNYMD*NSRFKARGFT*REGID 334
           ++   N TW +  +P+    +    +F +K  E  N +   +R  ARGFT +  ID
Sbjct: 915 NAHKINNTWTITKRPENKNIVDSRWVFSVKYNELGNPIRYKARLVARGFTQKYQID 970



 Score = 30.8 bits (68), Expect = 8.2
 Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 1/54 (1%)

Query: 3   QICKDSGIVRHKQIRGTPRENCLDH*RMDRKIMERFICLMSNANNSTLFWDETV 56
           Q C   GI  H  +  TP+ N +   RM R I E+   ++S A     FW E V
Sbjct: 562 QFCVKKGISYHLTVPHTPQLNGVSE-RMIRTITEKARTMVSGAKLDKSFWGEAV 614


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.361    0.161    0.572 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,281,415
Number of Sequences: 164201
Number of extensions: 1441699
Number of successful extensions: 5079
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 5071
Number of HSP's gapped (non-prelim): 9
length of query: 485
length of database: 59,974,054
effective HSP length: 114
effective length of query: 371
effective length of database: 41,255,140
effective search space: 15305656940
effective search space used: 15305656940
T: 11
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.9 bits)
S2: 68 (30.8 bits)


Medicago: description of AC147000.15