BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146941.12 - phase: 0
(146 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RSUA_HAEIN (P45124) Ribosomal small subunit pseudouridine syntha... 30 1.4
MAT1_ASTLO (P14761) Maturase-like protein 1 30 1.8
CATV_GVXN (Q9PYY5) Viral cathepsin (EC 3.4.22.50) (V-cath) (Cyst... 30 1.8
MM19_HUMAN (Q99542) Matrix metalloproteinase-19 precursor (EC 3.... 30 2.4
ITN1_XENLA (O42287) Intersectin 1 30 2.4
ITN1_HUMAN (Q15811) Intersectin 1 (SH3 domain-containing protein... 30 2.4
NIA1_PHAVU (P39865) Nitrate reductase [NADH] 1 (EC 1.7.1.1) (NR-1) 29 4.1
ERB1_YEAST (Q04660) Eukaryotic ribosome biogenesis protein 1 28 5.3
HSLU_HELPJ (Q9ZLW1) ATP-dependent hsl protease ATP-binding subun... 28 6.9
YS25_CAEEL (Q09366) Hypothetical protein ZK1320.5 in chromosome II 28 9.1
RTCR_ECOLI (P38035) Transcriptional regulatory protein rtcR 28 9.1
NIA_BETVE (P27783) Nitrate reductase [NAD(P)H] (EC 1.7.1.2) (NR) 28 9.1
ITN1_RAT (Q9WVE9) Intersectin 1 (EH domain and SH3 domain regula... 28 9.1
ITN1_MOUSE (Q9Z0R4) Intersectin 1 (EH and SH3 domains protein 1) 28 9.1
HSLU_HELPY (O25254) ATP-dependent hsl protease ATP-binding subun... 28 9.1
HLDE_HAEDU (Q7VM30) Bifunctional protein hldE [Includes: D-beta-... 28 9.1
>RSUA_HAEIN (P45124) Ribosomal small subunit pseudouridine synthase
A (EC 4.2.1.70) (16S pseudouridylate 516 synthase) (16S
pseudouridine 516 synthase) (Uracil hydrolyase)
Length = 232
Score = 30.4 bits (67), Expect = 1.4
Identities = 22/87 (25%), Positives = 44/87 (50%), Gaps = 6/87 (6%)
Query: 29 YFEATIVSCLESGKYVIRYKN---LLKDDESELLMETLFPKDLRPLPPRVHNPWRFELNQ 85
YFE +++ +E G+Y + K + +D+ + T++ PL ++H+ R +++
Sbjct: 48 YFEDELLTWIEEGQYFMLNKPQGCVCSNDDGDY--PTIYQFFDYPLAGKLHSAGRLDVDT 105
Query: 86 K-VDVFDNDGWWVGEIASEKILMEKSY 111
+ + +DG W I S K EK+Y
Sbjct: 106 TGLVLLTDDGQWSHRITSPKHHCEKTY 132
>MAT1_ASTLO (P14761) Maturase-like protein 1
Length = 456
Score = 30.0 bits (66), Expect = 1.8
Identities = 15/53 (28%), Positives = 30/53 (56%), Gaps = 4/53 (7%)
Query: 77 NPWRFELNQKVDVFDNDGWWVGEIASEKILMEKSYYYSVYF--DYCHQTIYYP 127
N W F+ +KV +FDNDG + + ++ ++ + Y V F + H+ +++P
Sbjct: 97 NNWIFQNLKKVSLFDNDGKII--VKKVPVISDRVWCYLVKFAIEPVHEALFHP 147
>CATV_GVXN (Q9PYY5) Viral cathepsin (EC 3.4.22.50) (V-cath)
(Cysteine proteinase) (CP)
Length = 346
Score = 30.0 bits (66), Expect = 1.8
Identities = 13/54 (24%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Query: 42 KYVIRYKNLLKDDESELLMETLFPKDLRPLPPR--VHNPWRFELNQKVDVFDND 93
++V++Y + KDD+ + +F ++L + R + + FE+N + D+ N+
Sbjct: 45 EFVVKYNKVYKDDQEKEARFEIFKQNLADINARNALEDSAMFEINSRADISSNE 98
>MM19_HUMAN (Q99542) Matrix metalloproteinase-19 precursor (EC
3.4.24.-) (MMP-19) (Matrix metalloproteinase RASI)
(MMP-18)
Length = 508
Score = 29.6 bits (65), Expect = 2.4
Identities = 13/33 (39%), Positives = 18/33 (54%)
Query: 64 FPKDLRPLPPRVHNPWRFELNQKVDVFDNDGWW 96
FPK L + P + + LNQKV +F G+W
Sbjct: 369 FPKKLNRVEPNLDAALYWPLNQKVFLFKGSGYW 401
>ITN1_XENLA (O42287) Intersectin 1
Length = 1270
Score = 29.6 bits (65), Expect = 2.4
Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Query: 60 METLFPKDLRPLPPRVHNPWRFELNQKVDVFDN-DGWWVGEIASEKILMEKSY 111
+E L + L P + N F N + V + D WW GE+ +K KSY
Sbjct: 897 VEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSY 949
>ITN1_HUMAN (Q15811) Intersectin 1 (SH3 domain-containing protein
1A) (SH3P17)
Length = 1721
Score = 29.6 bits (65), Expect = 2.4
Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Query: 60 METLFPKDLRPLPPRVHNPWRFELNQKVDVFDN-DGWWVGEIASEKILMEKSY 111
+E L + L P + N F N + V + D WW GE+ +K KSY
Sbjct: 913 VEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSY 965
>NIA1_PHAVU (P39865) Nitrate reductase [NADH] 1 (EC 1.7.1.1) (NR-1)
Length = 881
Score = 28.9 bits (63), Expect = 4.1
Identities = 15/47 (31%), Positives = 24/47 (50%)
Query: 15 KVEVCSKEEGFVGSYFEATIVSCLESGKYVIRYKNLLKDDESELLME 61
K VC ++G +G FE +SG ++ R K L K ES +++
Sbjct: 457 KTNVCKPKKGEIGIVFEHPTQPGNQSGGWMAREKQLEKSSESNPILK 503
>ERB1_YEAST (Q04660) Eukaryotic ribosome biogenesis protein 1
Length = 807
Score = 28.5 bits (62), Expect = 5.3
Identities = 14/27 (51%), Positives = 16/27 (58%)
Query: 48 KNLLKDDESELLMETLFPKDLRPLPPR 74
KN L D + L+ E PKDLRP P R
Sbjct: 402 KNKLNIDPNSLIPELPSPKDLRPFPIR 428
>HSLU_HELPJ (Q9ZLW1) ATP-dependent hsl protease ATP-binding subunit
hslU
Length = 443
Score = 28.1 bits (61), Expect = 6.9
Identities = 18/56 (32%), Positives = 28/56 (49%), Gaps = 2/56 (3%)
Query: 22 EEGFVGSYFEATIVSCLESGKYVIR--YKNLLKDDESELLMETLFPKDLRPLPPRV 75
E GFVG E+ + + + ++ +K LKD E ++E + K L PLP V
Sbjct: 90 EVGFVGRDVESMVRDLVNNSVLLVENEHKERLKDKIEEAVVEKIAKKLLPPLPSGV 145
>YS25_CAEEL (Q09366) Hypothetical protein ZK1320.5 in chromosome II
Length = 414
Score = 27.7 bits (60), Expect = 9.1
Identities = 14/51 (27%), Positives = 28/51 (54%)
Query: 83 LNQKVDVFDNDGWWVGEIASEKILMEKSYYYSVYFDYCHQTIYYPCDQIRV 133
LN + + G V + ++++I ++ S +VY+D+ H ++ PC I V
Sbjct: 142 LNSDDQLKEAAGLEVLQSSNDEISIKSSLIANVYYDFYHGILFLPCIIISV 192
>RTCR_ECOLI (P38035) Transcriptional regulatory protein rtcR
Length = 532
Score = 27.7 bits (60), Expect = 9.1
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 11/64 (17%)
Query: 28 SYFEATIVSCLESGKYVIRYKNLLKDDESELLMETLFPKDLRPLPPR---------VHNP 78
S + T+ C + + R + LL D S L ETL +D+ + P +HNP
Sbjct: 25 SKWRPTLCLCQQESLVIDRLE-LLHDARSRSLFETL-KRDIASVSPETEVVSVEIELHNP 82
Query: 79 WRFE 82
W FE
Sbjct: 83 WDFE 86
>NIA_BETVE (P27783) Nitrate reductase [NAD(P)H] (EC 1.7.1.2) (NR)
Length = 898
Score = 27.7 bits (60), Expect = 9.1
Identities = 15/36 (41%), Positives = 18/36 (49%)
Query: 15 KVEVCSKEEGFVGSYFEATIVSCLESGKYVIRYKNL 50
K VC G +G FE V ESG ++ R KNL
Sbjct: 470 KTNVCKAHMGEIGIAFEHPTVPGNESGGWMAREKNL 505
>ITN1_RAT (Q9WVE9) Intersectin 1 (EH domain and SH3 domain regulator
of endocytosis 1)
Length = 1217
Score = 27.7 bits (60), Expect = 9.1
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Query: 60 METLFPKDLRPLPPRVHNPWRFELNQKVDVFDN-DGWWVGEIASEKILMEKSY 111
+E L + L P + N F + + V + D WW GE+ +K KSY
Sbjct: 910 VEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWWFGEVQGQKGWFPKSY 962
>ITN1_MOUSE (Q9Z0R4) Intersectin 1 (EH and SH3 domains protein 1)
Length = 1714
Score = 27.7 bits (60), Expect = 9.1
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Query: 60 METLFPKDLRPLPPRVHNPWRFELNQKVDVFDN-DGWWVGEIASEKILMEKSY 111
+E L + L P + N F + + V + D WW GE+ +K KSY
Sbjct: 906 VEGLQAQALYPWRAKKDNHLNFNKSDVITVLEQQDMWWFGEVQGQKGWFPKSY 958
>HSLU_HELPY (O25254) ATP-dependent hsl protease ATP-binding subunit
hslU
Length = 443
Score = 27.7 bits (60), Expect = 9.1
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Query: 22 EEGFVGSYFEATIVSCLESGKYVIR--YKNLLKDDESELLMETLFPKDLRPLP 72
E GFVG E+ + + + ++ +K LKD E ++E + K L PLP
Sbjct: 90 EVGFVGRDVESMVRDLVNNSVLLVENEHKEKLKDKIEEAVIEKIAKKLLPPLP 142
>HLDE_HAEDU (Q7VM30) Bifunctional protein hldE [Includes:
D-beta-D-heptose 7-phosphate kinase (EC 2.7.1.-)
(D-beta-D-heptose 7-phosphotransferase);
D-beta-D-heptose 1-phosphate adenosyltransferase (EC
2.7.7.-)]
Length = 475
Score = 27.7 bits (60), Expect = 9.1
Identities = 22/68 (32%), Positives = 29/68 (42%), Gaps = 8/68 (11%)
Query: 30 FEATIVSCLESGKYVIRYKNLLKDDESELLMETLFPKD---LRPLPPRVHNPWRFELNQK 86
FEA + +CL V + L+ D E L+ T K LRP P H P Q
Sbjct: 199 FEAVVGACLNDNDIVSKGLKLINDLELSALLVTRSEKGMTLLRPNHPPFHLP-----TQA 253
Query: 87 VDVFDNDG 94
+V+D G
Sbjct: 254 KEVYDVTG 261
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.141 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,736,583
Number of Sequences: 164201
Number of extensions: 741869
Number of successful extensions: 1903
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 1896
Number of HSP's gapped (non-prelim): 18
length of query: 146
length of database: 59,974,054
effective HSP length: 100
effective length of query: 46
effective length of database: 43,553,954
effective search space: 2003481884
effective search space used: 2003481884
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146941.12