BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146940.1 - phase: 0
(285 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YGO4_YEAST (P53118) Hypothetical 78.1 kDa protein in TIP20-MRF1 ... 75 2e-13
YE7A_SCHPO (O14162) Hypothetical protein C4A8.10 in chromosome I 56 9e-08
Y193_HAEIN (Q57427) Putative esterase/lipase HI0193 (EC 3.1.-.-) 38 0.025
LIP_PSEFR (P08658) Lipase precursor (EC 3.1.1.3) (Triacylglycero... 35 0.27
LIP_RHIMI (P19515) Lipase precursor (EC 3.1.1.3) (Triacylglycero... 33 0.80
TRB1_METTH (O27696) Tryptophan synthase beta chain 1 (EC 4.2.1.20) 33 1.0
DPOL_BPB03 (Q37882) DNA polymerase (EC 2.7.7.7) (Early protein GP2) 33 1.0
PIP_LACHE (P52278) Proline iminopeptidase (EC 3.4.11.5) (PIP) (P... 32 1.8
P52K_MOUSE (Q9CUX1) 52 kDa repressor of the inhibitor of the pro... 31 4.0
DPOL_BPM2 (P19894) DNA polymerase (EC 2.7.7.7) 31 4.0
DNK2_SYNP7 (P50021) Chaperone protein dnaK2 (Heat shock protein ... 31 4.0
DNK2_SYNEL (Q8DI58) Chaperone protein dnaK2 (Heat shock protein ... 31 4.0
MYSC_DICDI (P42522) Myosin IC heavy chain 30 5.2
TP3B_MOUSE (Q9Z321) DNA topoisomerase III beta-1 (EC 5.99.1.2) 30 6.7
THIC_AGRT5 (Q8UCC9) Thiamine biosynthesis protein thiC 30 6.7
P52K_HUMAN (O43422) 52 kDa repressor of the inhibitor of the pro... 30 6.7
LIP_THELA (O59952) Lipase precursor (EC 3.1.1.3) (Triacylglycero... 30 6.7
LIPG_BOVIN (Q29458) Triacylglycerol lipase, pregastric precursor... 30 6.7
DNAK_BACTN (Q89YW6) Chaperone protein dnaK (Heat shock protein 7... 30 6.7
APCE_SYNY4 (Q02907) Phycobilisome 100.5 kDa core-membrane linker... 30 6.7
>YGO4_YEAST (P53118) Hypothetical 78.1 kDa protein in TIP20-MRF1
intergenic region
Length = 685
Score = 75.1 bits (183), Expect = 2e-13
Identities = 71/229 (31%), Positives = 98/229 (42%), Gaps = 48/229 (20%)
Query: 61 HLIIMVNGLIGSAH-NWKYAAKQFLK---RYPYDVIVHCSECNSSTLTFDGVDVTGNRLA 116
HL+++ +GL + + Y +Q K YP++ IV + T GV G RLA
Sbjct: 189 HLVVLTHGLHSNVSTDLVYIMEQIYKAQKNYPHEQIVVKGYRGNVCQTEKGVKYLGTRLA 248
Query: 117 EEVISVIKRHPSVRKISFIAHSLGGLIARYAIAKLYERDISKELSQGNVHCEGQISNQEC 176
E +I + S+RKISF+ HSLGGLI +AIA +YE
Sbjct: 249 EYIIQDLYDE-SIRKISFVGHSLGGLIQAFAIAYIYE----------------------- 284
Query: 177 HVRKYEGKIAGLEPINFITSATPHLG--CRGHKQVPLLCGFHSLEKTASRLSRFLGKTGK 234
Y + PINFIT A+P LG + +L F +GKTG+
Sbjct: 285 ---VYPWFFKKVNPINFITLASPLLGIVTDNPAYIKVLLSF-----------GVIGKTGQ 330
Query: 235 HLFLTDGKNEKPPLLLQMVRDSEDIKFMSALRSFKRRVAYANIRYDRIL 283
L L + PLL + + + LR FKRR YAN D I+
Sbjct: 331 DLGLENDVEVGKPLLYLL----SGLPLIEILRRFKRRTVYANAINDGIV 375
>YE7A_SCHPO (O14162) Hypothetical protein C4A8.10 in chromosome I
Length = 723
Score = 56.2 bits (134), Expect = 9e-08
Identities = 57/242 (23%), Positives = 99/242 (40%), Gaps = 45/242 (18%)
Query: 53 SGHRSNPTHLIIMVNGLIGSAH-NWKYAAKQFLK--RYPYDVIVHCSECNSSTLTFDGVD 109
S S +HL+++ +G+ + + +Y ++ ++ + +++V + T GV
Sbjct: 236 SSFHSKNSHLVVLTHGMHSNVGADMEYLKEKLIESSKSVKELVVVRGFTGNYCQTEKGVR 295
Query: 110 VTGNRLAEEVISVI----KRHPSVRKISFIAHSLGGLIARYAIAKLYERDISKELSQGNV 165
G RL E ++ + P IS +AHSLGGL+ YA+ +
Sbjct: 296 WLGKRLGEWLLDITGWGSASFPRYSHISVVAHSLGGLVQTYAVGYV-------------- 341
Query: 166 HCEGQISNQECHVRKYEGKIAGLEPINFITSATPHLGCRGHKQVPLLCGFHSLEKTASRL 225
H + + + P+ F+T ATP LG + G H +
Sbjct: 342 -----------HAKTHGAFFQAIHPVFFVTLATPWLG---------VAGEHPSYIGKALS 381
Query: 226 SRFLGKTGKHLFLT--DGKNEKPPLLLQMVRDSEDIKFMSALRSFKRRVAYANIRYDRIL 283
+GKTG+ L LT + E P L+ M F A+ F++R+ +AN D I+
Sbjct: 382 YGIIGKTGQDLSLTPLNHSIESRPFLVLM--SDPSTPFFQAVSFFEKRILFANTTNDYIV 439
Query: 284 LF 285
F
Sbjct: 440 PF 441
>Y193_HAEIN (Q57427) Putative esterase/lipase HI0193 (EC 3.1.-.-)
Length = 287
Score = 38.1 bits (87), Expect = 0.025
Identities = 27/88 (30%), Positives = 44/88 (49%), Gaps = 11/88 (12%)
Query: 60 THLIIMVNGLIGSAHNWKYAAKQFLKRYPY---DVIVHCSECNSSTLTFDGVDVTGNRLA 116
T ++I ++GL G N A+ F + Y D+ H +S + + +A
Sbjct: 46 TPVLIFIHGLFGDMDNLGVIARAFSEHYSILRIDLRNHGHSFHSEKMNYQ-------LMA 98
Query: 117 EEVISVIKRHPSVRKISFIAHSLGGLIA 144
E+VI+VI RH ++ K+ I HS+GG A
Sbjct: 99 EDVIAVI-RHLNLSKVILIGHSMGGKTA 125
>LIP_PSEFR (P08658) Lipase precursor (EC 3.1.1.3) (Triacylglycerol
lipase)
Length = 277
Score = 34.7 bits (78), Expect = 0.27
Identities = 24/96 (25%), Positives = 46/96 (47%), Gaps = 11/96 (11%)
Query: 58 NPTHLIIMVNGLIG----SAHNWKYAAKQFLKRYPYDVIVHCSECNSSTLTFDGVDVTGN 113
N + I++V+GL G +H++ + KQ L V V + + G+
Sbjct: 6 NTRYPILLVHGLFGFDRIGSHHYFHGIKQALNECGASVFVPIISAANDN------EARGD 59
Query: 114 RLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAIA 149
+L +++ + ++R ++++ I HS G L ARY A
Sbjct: 60 QLLKQIHN-LRRQVGAQRVNLIGHSQGALTARYVAA 94
>LIP_RHIMI (P19515) Lipase precursor (EC 3.1.1.3) (Triacylglycerol
lipase)
Length = 363
Score = 33.1 bits (74), Expect = 0.80
Identities = 19/58 (32%), Positives = 29/58 (49%), Gaps = 1/58 (1%)
Query: 113 NRLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAIAKLYERDISKELSQGNVHCEGQ 170
N L V+ K++PS K++ HSLGG A LY+R+ S ++ +GQ
Sbjct: 214 NELVATVLDQFKQYPSY-KVAVTGHSLGGATALLCALDLYQREEGLSSSNLFLYTQGQ 270
>TRB1_METTH (O27696) Tryptophan synthase beta chain 1 (EC 4.2.1.20)
Length = 392
Score = 32.7 bits (73), Expect = 1.0
Identities = 30/115 (26%), Positives = 53/115 (46%), Gaps = 16/115 (13%)
Query: 175 ECHVRKYEGKIAGLEPINFITSATPHLGCRGH--KQVPLLCGFHSLEKTASR--LSRFLG 230
E H+R+Y GK GL + + + LGCR + ++ L G H + T + L+R++G
Sbjct: 44 EYHLREYAGKPTGL---YYARNLSEKLGCRVYLKREDMLHTGAHKINNTIGQALLARYMG 100
Query: 231 KT--------GKHLFLTDGKNEKPPLLLQMVRDSEDIKFMSALRSFKRRVAYANI 277
KT G+H T + + + +ED++ L F+ V+ A +
Sbjct: 101 KTRIIAETGAGQHGIATAAAGALFGMDVDIYMGTEDVE-RQKLNVFRMEVSGAEV 154
>DPOL_BPB03 (Q37882) DNA polymerase (EC 2.7.7.7) (Early protein GP2)
Length = 572
Score = 32.7 bits (73), Expect = 1.0
Identities = 29/101 (28%), Positives = 44/101 (42%), Gaps = 17/101 (16%)
Query: 89 YDVIVHCSECNSSTLTFDGVDVTGNRLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAI 148
YD I++C D + +TG EV +IK +K+ + AH A+Y
Sbjct: 446 YDRIIYCDT--------DSIHLTGT----EVPEIIKDIVDPKKLGYWAHESTFKRAKYLR 493
Query: 149 AKLYERDI-SKELSQGNVHCEGQISNQECHVRKYEGKIAGL 188
K Y +DI +KE+ + C S E K+ K AG+
Sbjct: 494 QKTYIQDIYAKEVDGKLIEC----SPDEATTTKFSVKCAGM 530
>PIP_LACHE (P52278) Proline iminopeptidase (EC 3.4.11.5) (PIP)
(Prolyl aminopeptidase) (PAP)
Length = 294
Score = 32.0 bits (71), Expect = 1.8
Identities = 20/92 (21%), Positives = 45/92 (48%), Gaps = 2/92 (2%)
Query: 54 GHRSNPTHLIIMVNGLIGSAHNWKYAAKQFLKRYPYDVIVHCS-ECNSSTLTFDGVDVTG 112
G RS L+++ G GS+HN+ + ++ +I++ C S++ D ++
Sbjct: 21 GERSEKPPLVLLHGGP-GSSHNYFEVLDELAQKDGRRIIMYDQLGCGESSIPDDHPELYT 79
Query: 113 NRLAEEVISVIKRHPSVRKISFIAHSLGGLIA 144
+ + ++ H ++RK+ + S GG++A
Sbjct: 80 KETWVKELEALREHLALRKMHLLGQSWGGMLA 111
>P52K_MOUSE (Q9CUX1) 52 kDa repressor of the inhibitor of the
protein kinase (p58IPK-interacting protein) (58 kDa
interferon-induced protein kinase-interacting protein)
(P52rIPK) (THAP domain protein 0)
Length = 758
Score = 30.8 bits (68), Expect = 4.0
Identities = 20/63 (31%), Positives = 27/63 (42%), Gaps = 4/63 (6%)
Query: 71 GSAHNWKYAAKQFLKRYPYDVIVHCSECNSSTLTFDGVDVTGNRLA----EEVISVIKRH 126
G + K A + L++YP V CS C + V V G +A EEV S R
Sbjct: 331 GFSSKMKVVASRLLEKYPQAVYTLCSSCALNAWLAKSVPVIGVSVALGTIEEVCSFFHRS 390
Query: 127 PSV 129
P +
Sbjct: 391 PQL 393
>DPOL_BPM2 (P19894) DNA polymerase (EC 2.7.7.7)
Length = 572
Score = 30.8 bits (68), Expect = 4.0
Identities = 27/100 (27%), Positives = 42/100 (42%), Gaps = 15/100 (15%)
Query: 89 YDVIVHCSECNSSTLTFDGVDVTGNRLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAI 148
YD I++C D + +TG EV +IK +K+ + AH A+Y
Sbjct: 446 YDRIIYCDT--------DSIHLTGT----EVPEIIKDIVDPKKLGYWAHESTFKRAKYLR 493
Query: 149 AKLYERDISKELSQGNVHCEGQISNQECHVRKYEGKIAGL 188
K Y +DI + G + + S E K+ K AG+
Sbjct: 494 QKTYIQDIYVKEVDGKLK---ECSPDEATTTKFSVKCAGM 530
>DNK2_SYNP7 (P50021) Chaperone protein dnaK2 (Heat shock protein
70-2) (Heat shock 70 kDa protein 2) (HSP70-2)
Length = 634
Score = 30.8 bits (68), Expect = 4.0
Identities = 36/161 (22%), Positives = 63/161 (38%), Gaps = 26/161 (16%)
Query: 49 VVDASGHRSNPTHLIIMVNG--LIG---------SAHNWKYAAKQFLKRYPYDVIVHCSE 97
+ +A G R+ P+ + N L+G + N Y+ K+F+ R P +V
Sbjct: 27 IANAEGFRTTPSVVAFAKNQDRLVGQIAKRQAVMNPENTFYSVKRFIGRRPDEVT----- 81
Query: 98 CNSSTLTFDGVDVTGNRLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAIAKLYERDIS 157
N T VD +GN +K S F + + R +A+ + +
Sbjct: 82 -NELTEVAYKVDTSGN--------AVKLDSSNAGKQFAPEEISAQVLR-KLAEDASKYLG 131
Query: 158 KELSQGNVHCEGQISNQECHVRKYEGKIAGLEPINFITSAT 198
+ ++Q + ++ + K GKIAGLE + I T
Sbjct: 132 ETVTQAVITVPAYFNDSQRQATKDAGKIAGLEVLRIINEPT 172
>DNK2_SYNEL (Q8DI58) Chaperone protein dnaK2 (Heat shock protein
70-2) (Heat shock 70 kDa protein 2) (HSP70-2)
Length = 640
Score = 30.8 bits (68), Expect = 4.0
Identities = 38/164 (23%), Positives = 71/164 (43%), Gaps = 32/164 (19%)
Query: 49 VVDASGHRSNPTHLIIMVNG--LIG---------SAHNWKYAAKQFLKRYPYDVIVHCSE 97
+ +A G R+ P+ + NG L+G + N Y+ K+F+ R +D + H
Sbjct: 27 IANAEGFRTTPSVVAYTKNGDRLVGQIAKRQAVMNPENTFYSVKRFIGRR-FDEVTH--- 82
Query: 98 CNSSTLTFDGVDVTGNRLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAIAKLYE---R 154
++ +++ ++V GN V P++ K F + + R KL E +
Sbjct: 83 -EATEVSYKVLNVNGN--------VKLDCPALGK-QFAPEEISAQVLR----KLKEDASK 128
Query: 155 DISKELSQGNVHCEGQISNQECHVRKYEGKIAGLEPINFITSAT 198
+ +E++Q + ++ + K GKIAGLE + I T
Sbjct: 129 YLGEEVTQAVITVPAYFNDSQRQATKDAGKIAGLEVLRIINEPT 172
>MYSC_DICDI (P42522) Myosin IC heavy chain
Length = 1181
Score = 30.4 bits (67), Expect = 5.2
Identities = 28/109 (25%), Positives = 48/109 (43%), Gaps = 3/109 (2%)
Query: 169 GQISNQECHVRKYEGKIAGLEPINFITSATPHLGCRGHKQVPLLCGFHSLEKTASRLSRF 228
G I + + K +I + NF+ P + K++P GF ++ A+ L
Sbjct: 519 GMIEKNKDTLLKDHLEILQMSANNFLVGLFPDVIDTDSKKLPSTAGF-KIKSQAAELVAT 577
Query: 229 LGKTGKHLFLTDGKNE-KPPLLLQMVRDSEDIKFMSALRSFK-RRVAYA 275
L K+ H T N+ K P +L+ R +K++ L + K RR +A
Sbjct: 578 LMKSTPHYIRTIKPNDLKKPNILEGGRVLHQVKYLGLLDNIKVRRAGFA 626
>TP3B_MOUSE (Q9Z321) DNA topoisomerase III beta-1 (EC 5.99.1.2)
Length = 862
Score = 30.0 bits (66), Expect = 6.7
Identities = 19/49 (38%), Positives = 29/49 (58%), Gaps = 3/49 (6%)
Query: 154 RDISKELSQGNVHCEGQISNQECHVRKYEGKIAGLEPINF-ITSATPHL 201
+ I+K LS+GN+ + N C V KY G AG +P++F +TS H+
Sbjct: 15 QSIAKILSRGNMSSHKGL-NGACSVHKYTGTFAG-QPVHFKMTSVCGHV 61
>THIC_AGRT5 (Q8UCC9) Thiamine biosynthesis protein thiC
Length = 607
Score = 30.0 bits (66), Expect = 6.7
Identities = 12/33 (36%), Positives = 21/33 (63%)
Query: 47 IEVVDASGHRSNPTHLIIMVNGLIGSAHNWKYA 79
+ V D+SG ++P+H +++ NGL H+W A
Sbjct: 51 VTVYDSSGPYTDPSHPVLIENGLPRLRHDWVVA 83
>P52K_HUMAN (O43422) 52 kDa repressor of the inhibitor of the
protein kinase (p58IPK-interacting protein) (58 kDa
interferon-induced protein kinase-interacting protein)
(P52rIPK) (Death associated protein 4) (THAP domain
protein 0)
Length = 761
Score = 30.0 bits (66), Expect = 6.7
Identities = 19/63 (30%), Positives = 27/63 (42%), Gaps = 4/63 (6%)
Query: 71 GSAHNWKYAAKQFLKRYPYDVIVHCSECNSSTLTFDGVDVTGNRLA----EEVISVIKRH 126
G + K A + L++YP + CS C + V V G +A EEV S R
Sbjct: 335 GFSSKMKVVASRLLEKYPQAIYTLCSSCALNMWLAKSVPVMGVSVALGTIEEVCSFFHRS 394
Query: 127 PSV 129
P +
Sbjct: 395 PQL 397
>LIP_THELA (O59952) Lipase precursor (EC 3.1.1.3) (Triacylglycerol
lipase)
Length = 291
Score = 30.0 bits (66), Expect = 6.7
Identities = 18/57 (31%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Query: 95 CSECNSSTLTFDGVDVTGNRLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAIAKL 151
CS C + L ++V ++ HP R + F HSLGG +A A A L
Sbjct: 126 CSGCRGHDGFTSSWRSVADTLRQKVEDAVREHPDYRVV-FTGHSLGGALATVAGADL 181
>LIPG_BOVIN (Q29458) Triacylglycerol lipase, pregastric precursor
(EC 3.1.1.3) (Pregastric lipase) (GL) (Pregastric
esterase) (PGE)
Length = 397
Score = 30.0 bits (66), Expect = 6.7
Identities = 32/131 (24%), Positives = 48/131 (36%), Gaps = 22/131 (16%)
Query: 41 TGDGFDIEVVDASGHRSNPTHL-----IIMVNGLIGSAHNW-----KYAAKQFLKRYPYD 90
T DG+ ++V ++N HL + + +GL+GSA NW K + L YD
Sbjct: 52 TADGYILQVYRIPHGKNNANHLGQRPVVFLQHGLLGSATNWISNLPKNSLGFLLADAGYD 111
Query: 91 VIVHCSECN------------SSTLTFDGVDVTGNRLAEEVISVIKRHPSVRKISFIAHS 138
V + S N S D I I R +K+ ++ HS
Sbjct: 112 VWLGNSRGNTWAQEHLYYSPDSPEFWAFSFDEMAEYDLPSTIDFILRRTGQKKLHYVGHS 171
Query: 139 LGGLIARYAIA 149
G I A +
Sbjct: 172 QGTTIGFIAFS 182
>DNAK_BACTN (Q89YW6) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 638
Score = 30.0 bits (66), Expect = 6.7
Identities = 33/158 (20%), Positives = 61/158 (37%), Gaps = 32/158 (20%)
Query: 43 DGFDIEVVDASGHRS--NPTHLIIMVNGLIGSAHNWKYAAKQFLKRYPYDVIVHCSECNS 100
DG + +V D + ++ NPT I + +G NW K+ + R PY V+ + N+
Sbjct: 44 DGGERKVGDPAKRQAITNPTRTIFSIKRFMGE--NWDQVQKE-VTRVPYKVVK--GDNNT 98
Query: 101 STLTFDGVDVTGNRLAEEVISVIKRHPSVRKISFIAHSLGGLIARYAIAKLYERDISKEL 160
+ DG T ++ ++ +K K E + +E+
Sbjct: 99 PRVDIDGRLYTPQEISAMILQKMK-------------------------KTAEDYLGQEV 133
Query: 161 SQGNVHCEGQISNQECHVRKYEGKIAGLEPINFITSAT 198
++ + S+ + K G+IAGLE + T
Sbjct: 134 TEAVITVPAYFSDSQRQATKEAGQIAGLEVKRIVNEPT 171
>APCE_SYNY4 (Q02907) Phycobilisome 100.5 kDa core-membrane linker
polypeptide (L-CM 100.5) (Anchor polypeptide)
Length = 896
Score = 30.0 bits (66), Expect = 6.7
Identities = 12/44 (27%), Positives = 27/44 (61%)
Query: 143 IARYAIAKLYERDISKELSQGNVHCEGQISNQECHVRKYEGKIA 186
+ + A +++ERDI+K SQ + E Q+ N + ++++ ++A
Sbjct: 281 VIKAAYRQIFERDITKAYSQSISYLESQVRNGDISMKEFVRRLA 324
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.138 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,377,292
Number of Sequences: 164201
Number of extensions: 1375607
Number of successful extensions: 3215
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 17
Number of HSP's that attempted gapping in prelim test: 3205
Number of HSP's gapped (non-prelim): 23
length of query: 285
length of database: 59,974,054
effective HSP length: 109
effective length of query: 176
effective length of database: 42,076,145
effective search space: 7405401520
effective search space used: 7405401520
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 65 (29.6 bits)
Medicago: description of AC146940.1