BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146864.1 + phase: 0
(194 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YV03_HUMAN (Q9H4I3) Hypothetical protein PP2447 43 5e-04
YD65_METJA (Q58760) Hypothetical protein MJ1365 42 9e-04
YV03_MOUSE (Q99JY4) Hypothetical protein PP2447 homolog 41 0.002
RL15_RICPR (Q9ZCS4) 50S ribosomal protein L15 32 0.93
EF2_PYRAB (Q9V1Z8) Elongation factor 2 (EF-2) 30 3.5
AAC4_MOUSE (P57780) Alpha-actinin 4 (Non-muscle alpha-actinin 4)... 30 4.6
AAC4_HUMAN (O43707) Alpha-actinin 4 (Non-muscle alpha-actinin 4)... 30 4.6
AAC1_MOUSE (Q7TPR4) Alpha-actinin 1 (Alpha-actinin cytoskeletal ... 30 4.6
EF2_PYRHO (O59521) Elongation factor 2 (EF-2) 29 6.0
AAC1_CHICK (P05094) Alpha-actinin 1 (Alpha-actinin cytoskeletal ... 29 6.0
POLN_SFV (P08411) Nonstructural polyprotein (Polyprotein nsP1234... 29 7.8
MSH6_DROME (Q9VUM0) Probable DNA mismatch repair protein MSH6 29 7.8
KF4A_HUMAN (O95239) Chromosome-associated kinesin KIF4A (Chromok... 29 7.8
GBAZ_HUMAN (P19086) Guanine nucleotide-binding protein G(z), alp... 29 7.8
AAC4_RAT (Q9QXQ0) Alpha-actinin 4 (Non-muscle alpha-actinin 4) (... 29 7.8
>YV03_HUMAN (Q9H4I3) Hypothetical protein PP2447
Length = 376
Score = 42.7 bits (99), Expect = 5e-04
Identities = 28/84 (33%), Positives = 48/84 (56%), Gaps = 12/84 (14%)
Query: 43 KERQRRLELPKELTKNVIQLSCDSMEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPEI 102
K R++R LP+ +T+ V E G VY+VGT H + +S + V + ++ ++P++
Sbjct: 52 KRRRQRPNLPRTVTQLVA-------EDGS--RVYVVGTAHFSDDSKRDVVKTIREVQPDV 102
Query: 103 VFLELCSDRQEVLLHDNMEVSTIL 126
V +ELC R +L M+ ST+L
Sbjct: 103 VVVELCQYRVSML---KMDESTLL 123
>YD65_METJA (Q58760) Hypothetical protein MJ1365
Length = 397
Score = 42.0 bits (97), Expect = 9e-04
Identities = 21/49 (42%), Positives = 33/49 (66%), Gaps = 1/49 (2%)
Query: 73 CDVYLVGTFHGNKESSKQVEEIVKFLKPEIVFLELCSDRQEVLLHDNME 121
CD+YL+GT H +K+S ++VE+I+ + PE + +EL DR+ L N E
Sbjct: 17 CDIYLIGTAHVSKDSIEEVEKIISSVSPEGIAVEL-DDRRFFSLITNEE 64
>YV03_MOUSE (Q99JY4) Hypothetical protein PP2447 homolog
Length = 376
Score = 41.2 bits (95), Expect = 0.002
Identities = 25/77 (32%), Positives = 43/77 (55%), Gaps = 6/77 (7%)
Query: 42 EKERQRRLELPKELTKNVIQLSCDSMEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPE 101
E + +RR E P L + V QL + + VY+VGT H + +S + V + ++ ++P+
Sbjct: 47 EMKLKRRRERPN-LPRTVTQLVAEDGSR-----VYVVGTAHFSDDSKRDVVKTIREVQPD 100
Query: 102 IVFLELCSDRQEVLLHD 118
+V +ELC R +L D
Sbjct: 101 VVVVELCQYRVSMLKMD 117
>RL15_RICPR (Q9ZCS4) 50S ribosomal protein L15
Length = 150
Score = 32.0 bits (71), Expect = 0.93
Identities = 17/37 (45%), Positives = 23/37 (61%), Gaps = 3/37 (8%)
Query: 141 FSCIDNQHYHSIINFFNFSQALTMGEM--NDAMRKEK 175
F CI + Y+ IIN +N +ALT G + ND + KEK
Sbjct: 65 FKCISTKKYN-IINIYNIEEALTDGRLSTNDIITKEK 100
>EF2_PYRAB (Q9V1Z8) Elongation factor 2 (EF-2)
Length = 732
Score = 30.0 bits (66), Expect = 3.5
Identities = 15/51 (29%), Positives = 26/51 (50%)
Query: 48 RLELPKELTKNVIQLSCDSMEKGGVCDVYLVGTFHGNKESSKQVEEIVKFL 98
R++ PKE+ K + +L D G+ DVY F N + + + E++ L
Sbjct: 511 RIKNPKEVAKKLAELGMDYEIARGIVDVYNGNMFLDNTKGVQYLNEVMDLL 561
>AAC4_MOUSE (P57780) Alpha-actinin 4 (Non-muscle alpha-actinin 4)
(F-actin cross linking protein)
Length = 912
Score = 29.6 bits (65), Expect = 4.6
Identities = 23/85 (27%), Positives = 37/85 (43%), Gaps = 4/85 (4%)
Query: 67 MEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPEIVFLELCSDR-QEVLLHDNMEVSTI 125
ME+ G + + GT KQ E + KP + LE QE L+ DN +
Sbjct: 670 MEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPSLDLLEQQHQLIQEALIFDNKHTNYT 729
Query: 126 LVHIS---DNAIMTIRCSFSCIDNQ 147
+ HI + + TI + + ++NQ
Sbjct: 730 MEHIRVGWEQLLTTIARTINEVENQ 754
>AAC4_HUMAN (O43707) Alpha-actinin 4 (Non-muscle alpha-actinin 4)
(F-actin cross linking protein)
Length = 911
Score = 29.6 bits (65), Expect = 4.6
Identities = 23/85 (27%), Positives = 37/85 (43%), Gaps = 4/85 (4%)
Query: 67 MEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPEIVFLELCSDR-QEVLLHDNMEVSTI 125
ME+ G + + GT KQ E + KP + LE QE L+ DN +
Sbjct: 669 MEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYT 728
Query: 126 LVHIS---DNAIMTIRCSFSCIDNQ 147
+ HI + + TI + + ++NQ
Sbjct: 729 MEHIRVGWEQLLTTIARTINEVENQ 753
>AAC1_MOUSE (Q7TPR4) Alpha-actinin 1 (Alpha-actinin cytoskeletal
isoform) (Non-muscle alpha-actinin 1) (F-actin cross
linking protein)
Length = 892
Score = 29.6 bits (65), Expect = 4.6
Identities = 24/86 (27%), Positives = 41/86 (46%), Gaps = 6/86 (6%)
Query: 67 MEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPEIVFLELCSDR--QEVLLHDNMEVST 124
ME+ G + + GT +Q E+ + KP+I LE C + QE L+ DN +
Sbjct: 650 MEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLE-CDHQLIQEALIFDNKHTNY 708
Query: 125 ILVHIS---DNAIMTIRCSFSCIDNQ 147
+ HI + + TI + + ++NQ
Sbjct: 709 NMEHIRVGWEQLLTTIARTINEVENQ 734
>EF2_PYRHO (O59521) Elongation factor 2 (EF-2)
Length = 732
Score = 29.3 bits (64), Expect = 6.0
Identities = 14/51 (27%), Positives = 26/51 (50%)
Query: 48 RLELPKELTKNVIQLSCDSMEKGGVCDVYLVGTFHGNKESSKQVEEIVKFL 98
R++ PKE+ K + +L D G+ D+Y F N + + + E++ L
Sbjct: 511 RVKNPKEVAKKLAELGMDYEIARGIVDIYNGNMFIDNTKGVQYLNEVMDLL 561
>AAC1_CHICK (P05094) Alpha-actinin 1 (Alpha-actinin cytoskeletal
isoform) (Non-muscle alpha-actinin 1) (F-actin cross
linking protein)
Length = 893
Score = 29.3 bits (64), Expect = 6.0
Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 4/85 (4%)
Query: 67 MEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPEIVFLELCSDR-QEVLLHDNMEVSTI 125
ME+ G + + GT +Q E+ + KP+I LE + QE L+ DN +
Sbjct: 651 MEEIGRISIEMHGTLEDQLNHLRQYEKSIVNYKPKIDQLEGDHQQIQEALIFDNKHTNYT 710
Query: 126 LVHIS---DNAIMTIRCSFSCIDNQ 147
+ HI + + TI + + ++NQ
Sbjct: 711 MEHIRVGWEQLLTTIARTINEVENQ 735
>POLN_SFV (P08411) Nonstructural polyprotein (Polyprotein nsP1234)
[Contains: Nonstructural protein NSP1; Nonstructural
protein NSP2; Nonstructural protein NSP3; Nonstructural
protein NSP4]
Length = 2431
Score = 28.9 bits (63), Expect = 7.8
Identities = 22/93 (23%), Positives = 40/93 (42%), Gaps = 13/93 (13%)
Query: 13 RLRKVIQTTFTTLPCFSTTSAAFCRDQSPEKERQRRLEL---------PKELTKNVIQLS 63
RL++ + FT + +CRD+S EK+ Q +++ ELT +++++
Sbjct: 1455 RLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDMRTAVELLNDDVELTTDLVRVH 1514
Query: 64 CDSMEKG----GVCDVYLVGTFHGNKESSKQVE 92
DS G D L F G K + ++
Sbjct: 1515 PDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAID 1547
>MSH6_DROME (Q9VUM0) Probable DNA mismatch repair protein MSH6
Length = 1190
Score = 28.9 bits (63), Expect = 7.8
Identities = 11/36 (30%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
Query: 132 NAIMTIRCSFSCIDNQHYHSIINFFNFSQALTMGEM 167
N + ++C + + HYH++I+FF+ + +T+G M
Sbjct: 1057 NFLANLKCR--TLFSTHYHNLIDFFHNDKRITLGHM 1090
>KF4A_HUMAN (O95239) Chromosome-associated kinesin KIF4A
(Chromokinesin)
Length = 1232
Score = 28.9 bits (63), Expect = 7.8
Identities = 23/114 (20%), Positives = 49/114 (42%), Gaps = 8/114 (7%)
Query: 3 VLRSRCCLSTRLRKVIQTTFTTLPCFSTT--SAAFCRDQSPE--KERQRRLELPKELTKN 58
+L ++C L + +++ + S+ S C D +ER E+ EL
Sbjct: 860 ILEAKCALKYLIGELVSSKIQVSKLESSLKQSKTSCADMQKMLFEERNHFAEIETELQAE 919
Query: 59 VIQLSCDSMEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPEIVFLELCSDRQ 112
++++ EK +YL+ ++ + KQ+EE V + +++ C D +
Sbjct: 920 LVRMEQQHQEKV----LYLLSQLQQSQMAEKQLEESVSEKEQQLLSTLKCQDEE 969
>GBAZ_HUMAN (P19086) Guanine nucleotide-binding protein G(z), alpha
subunit (G(x) alpha chain) (Gz-alpha)
Length = 354
Score = 28.9 bits (63), Expect = 7.8
Identities = 34/158 (21%), Positives = 71/158 (44%), Gaps = 18/158 (11%)
Query: 36 CRDQSPEKERQRRLELPKELTKNVIQLSCDSMEKGGVCDVYLVGTFHGNKESSKQVEEIV 95
CR S EKE RR + + ++ L +S + + L+GT + K + + + +
Sbjct: 2 CRQSSEEKEAARR---SRRIDRH---LRSESQRQRREIKLLLLGTSNSGKST---IVKQM 52
Query: 96 KFLKPEIVFLELCSDRQEVLLHDNMEVSTILVHISDNAIMTIRCSFSCIDNQHYHSIINF 155
K + LE C + + +++++ ++ T ++ A+ +R F D + Y ++ F
Sbjct: 53 KIIHSGGFNLEACKEYKPLIIYNAIDSLTRII----RALAALRIDFHNPD-RAYDAVQLF 107
Query: 156 FNFSQALTMGEMNDAMRKEKYSIFRMVTSWLDAEACFN 193
A + GE+ E + R + + A+ACF+
Sbjct: 108 ALTGPAESKGEIT----PELLGVMRRLWADPGAQACFS 141
>AAC4_RAT (Q9QXQ0) Alpha-actinin 4 (Non-muscle alpha-actinin 4)
(F-actin cross linking protein)
Length = 911
Score = 28.9 bits (63), Expect = 7.8
Identities = 22/85 (25%), Positives = 37/85 (42%), Gaps = 4/85 (4%)
Query: 67 MEKGGVCDVYLVGTFHGNKESSKQVEEIVKFLKPEIVFLELCSDR-QEVLLHDNMEVSTI 125
ME+ G + + GT KQ E + KP + LE QE L+ DN +
Sbjct: 669 MEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYT 728
Query: 126 LVHIS---DNAIMTIRCSFSCIDNQ 147
+ H+ + + TI + + ++NQ
Sbjct: 729 MEHLRVGWEQLLTTIARTINEVENQ 753
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.135 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,515,135
Number of Sequences: 164201
Number of extensions: 761392
Number of successful extensions: 2617
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2612
Number of HSP's gapped (non-prelim): 15
length of query: 194
length of database: 59,974,054
effective HSP length: 104
effective length of query: 90
effective length of database: 42,897,150
effective search space: 3860743500
effective search space used: 3860743500
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146864.1