BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146862.9 + phase: 0
(100 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TP3A_DROME (Q9NG98) DNA topoisomerase III alpha (EC 5.99.1.2) 39 0.003
TP3A_HUMAN (Q13472) DNA topoisomerase III alpha (EC 5.99.1.2) 36 0.014
TP3A_MOUSE (O70157) DNA topoisomerase III alpha (EC 5.99.1.2) 35 0.025
TOP3_CAEEL (O61660) DNA topoisomerase III (EC 5.99.1.2) 31 0.47
CJ24_HUMAN (Q6ZVD7) Hypothetical protein C10orf24 29 1.8
RSNB_MOUSE (Q99P86) Resistin-like beta precursor (RELMbeta) (Cys... 28 3.0
PCLO_MOUSE (Q9QYX7) Piccolo protein (Presynaptic cytomatrix prot... 28 5.2
PCLO_HUMAN (Q9Y6V0) Piccolo protein (Aczonin) 28 5.2
DAPA_AQUAE (O67216) Dihydrodipicolinate synthase (EC 4.2.1.52) (... 28 5.2
UL16_HHV6U (P24442) Protein U65 27 6.7
PCLO_RAT (Q9JKS6) Piccolo protein (Multidomain presynaptic cytom... 27 6.7
PCLO_CHICK (Q9PU36) Piccolo protein (Aczonin) (Fragment) 27 6.7
>TP3A_DROME (Q9NG98) DNA topoisomerase III alpha (EC 5.99.1.2)
Length = 1250
Score = 38.5 bits (88), Expect = 0.003
Identities = 18/42 (42%), Positives = 23/42 (53%), Gaps = 4/42 (9%)
Query: 56 CYCGVKSSRGMVRKPGPKQGSLFFGCGNWTATRGARCHYFEW 97
C CG +S+ VRK GP QG F+ C TR C +F+W
Sbjct: 1152 CNCGQLASQLTVRKDGPNQGRPFYAC----PTREKSCGFFKW 1189
Score = 35.4 bits (80), Expect = 0.025
Identities = 23/93 (24%), Positives = 39/93 (41%), Gaps = 12/93 (12%)
Query: 13 IMWQTADRSLIWKQSPEQP----SAYPHFPFKARDMNTPIIQYHHP---CCYCGVKSSRG 65
++W T +R+ + +P P + P + RD P C C + +
Sbjct: 986 MLWGTRERASLGTAAPTPPPKPAAKRPRWDSVERDSTPPSSVPESETVLCTGCQQPARQN 1045
Query: 66 MVRKPGPKQGSLFFGCGNWTATRGARCHYFEWA 98
VRK GP G L++ C + C++F+WA
Sbjct: 1046 TVRKNGPNLGRLYYKC-----PKPDECNFFQWA 1073
>TP3A_HUMAN (Q13472) DNA topoisomerase III alpha (EC 5.99.1.2)
Length = 1001
Score = 36.2 bits (82), Expect = 0.014
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 6/45 (13%)
Query: 56 CYCGVKSSRGMVRKPGPKQGSLFFGCGNWTATRGARCHYFEWASA 100
C CG ++ VRK GP +G FF C G C++F WA +
Sbjct: 813 CNCGQEAVLLTVRKEGPNRGRQFFKC------NGGSCNFFLWADS 851
Score = 30.4 bits (67), Expect = 0.80
Identities = 14/42 (33%), Positives = 19/42 (44%), Gaps = 3/42 (7%)
Query: 56 CYCGVKSSRGMVRKPGPKQGSLFFGCGNWTATRGARCHYFEW 97
C C S V+K GP +G F C R +C +F+W
Sbjct: 897 CLCSQPSVTRTVQKDGPNKGRQFHTCAK---PREQQCGFFQW 935
>TP3A_MOUSE (O70157) DNA topoisomerase III alpha (EC 5.99.1.2)
Length = 1003
Score = 35.4 bits (80), Expect = 0.025
Identities = 17/45 (37%), Positives = 23/45 (50%), Gaps = 6/45 (13%)
Query: 56 CYCGVKSSRGMVRKPGPKQGSLFFGCGNWTATRGARCHYFEWASA 100
C CG ++ VRK GP QG F+ C N C++F WA +
Sbjct: 815 CNCGREAVLLTVRKQGPNQGRHFYKCSN------GDCNFFLWADS 853
Score = 31.6 bits (70), Expect = 0.36
Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 3/42 (7%)
Query: 56 CYCGVKSSRGMVRKPGPKQGSLFFGCGNWTATRGARCHYFEW 97
C CG + V+K GP +G F C R +C +F+W
Sbjct: 899 CLCGQPAVTRTVQKDGPNKGRQFHTCAK---PREQQCGFFQW 937
>TOP3_CAEEL (O61660) DNA topoisomerase III (EC 5.99.1.2)
Length = 759
Score = 31.2 bits (69), Expect = 0.47
Identities = 14/45 (31%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
Query: 56 CYCG--VKSSRGMVRKPGPKQGSLFFGCGNWTATRGARCHYFEWA 98
C C +++ +V+K GP +G F+ C + T +C++F+WA
Sbjct: 716 CQCPEPMRAVTKVVQKEGPNKGKKFYTC-SLPYTSSEKCNFFKWA 759
>CJ24_HUMAN (Q6ZVD7) Hypothetical protein C10orf24
Length = 879
Score = 29.3 bits (64), Expect = 1.8
Identities = 16/56 (28%), Positives = 24/56 (42%), Gaps = 7/56 (12%)
Query: 5 FKFSITNSIMWQTADRSLIWKQSPEQPSAYPHFPFKARDMNT---PI----IQYHH 53
F+ + + T + W QSP+ HFP K + NT P+ +QY H
Sbjct: 755 FEAEVIQDTIGDTGKKPASWSQSPQNQEMRKHFPQKFQLFNTSHMPVLAQDVQYEH 810
>RSNB_MOUSE (Q99P86) Resistin-like beta precursor (RELMbeta)
(Cysteine-rich secreted protein FIZZ2) (Cysteine-rich
secreted protein A12-beta)
Length = 105
Score = 28.5 bits (62), Expect = 3.0
Identities = 11/26 (42%), Positives = 14/26 (53%), Gaps = 4/26 (15%)
Query: 79 FGCGNWTATRGARCH----YFEWASA 100
+GCG+W G CH +WASA
Sbjct: 74 YGCGSWDIRNGNTCHCQCSVMDWASA 99
>PCLO_MOUSE (Q9QYX7) Piccolo protein (Presynaptic cytomatrix protein)
(Aczonin) (Brain-derived HLMN protein)
Length = 5038
Score = 27.7 bits (60), Expect = 5.2
Identities = 20/69 (28%), Positives = 31/69 (43%), Gaps = 4/69 (5%)
Query: 10 TNSIMWQTADRSLIWKQSPE----QPSAYPHFPFKARDMNTPIIQYHHPCCYCGVKSSRG 65
T SI + S +QSP+ + ++ FP ++DM P I+ H SS G
Sbjct: 4727 TESIEHGKSHSSQNSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEG 4786
Query: 66 MVRKPGPKQ 74
+R GP +
Sbjct: 4787 HLRSHGPSR 4795
>PCLO_HUMAN (Q9Y6V0) Piccolo protein (Aczonin)
Length = 5183
Score = 27.7 bits (60), Expect = 5.2
Identities = 20/69 (28%), Positives = 31/69 (43%), Gaps = 4/69 (5%)
Query: 10 TNSIMWQTADRSLIWKQSPE----QPSAYPHFPFKARDMNTPIIQYHHPCCYCGVKSSRG 65
T SI + S +QSP+ + ++ FP ++DM P I+ H SS G
Sbjct: 4691 TESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEG 4750
Query: 66 MVRKPGPKQ 74
+R GP +
Sbjct: 4751 HLRSHGPSR 4759
>DAPA_AQUAE (O67216) Dihydrodipicolinate synthase (EC 4.2.1.52)
(DHDPS)
Length = 294
Score = 27.7 bits (60), Expect = 5.2
Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 3/44 (6%)
Query: 18 ADRSLI---WKQSPEQPSAYPHFPFKARDMNTPIIQYHHPCCYC 58
AD +L+ + P Q Y HF A++++ PII Y+ P C
Sbjct: 96 ADGALVVVPYYNKPTQRGLYEHFKTVAQEVDIPIIIYNIPSRTC 139
>UL16_HHV6U (P24442) Protein U65
Length = 335
Score = 27.3 bits (59), Expect = 6.7
Identities = 14/55 (25%), Positives = 25/55 (45%)
Query: 46 TPIIQYHHPCCYCGVKSSRGMVRKPGPKQGSLFFGCGNWTATRGARCHYFEWASA 100
TP + Y + C +++R V+ G + + GCG W G + + +A A
Sbjct: 133 TPEVIYENCSIVCPEEANRLTVKGSGDNKLTPLGGCGAWCLKNGGDLYIYTFALA 187
>PCLO_RAT (Q9JKS6) Piccolo protein (Multidomain presynaptic cytomatrix
protein)
Length = 5085
Score = 27.3 bits (59), Expect = 6.7
Identities = 13/38 (34%), Positives = 18/38 (47%)
Query: 37 FPFKARDMNTPIIQYHHPCCYCGVKSSRGMVRKPGPKQ 74
FP ++DM P I+ H SS G +R GP +
Sbjct: 4805 FPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSR 4842
>PCLO_CHICK (Q9PU36) Piccolo protein (Aczonin) (Fragment)
Length = 5120
Score = 27.3 bits (59), Expect = 6.7
Identities = 13/38 (34%), Positives = 18/38 (47%)
Query: 37 FPFKARDMNTPIIQYHHPCCYCGVKSSRGMVRKPGPKQ 74
FP ++DM P I+ H SS G +R GP +
Sbjct: 4779 FPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSR 4816
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.137 0.491
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,365,786
Number of Sequences: 164201
Number of extensions: 482098
Number of successful extensions: 1263
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 1250
Number of HSP's gapped (non-prelim): 15
length of query: 100
length of database: 59,974,054
effective HSP length: 76
effective length of query: 24
effective length of database: 47,494,778
effective search space: 1139874672
effective search space used: 1139874672
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146862.9