BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146790.4 - phase: 0
(147 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ABP1_SCHPO (P49777) ARS-binding protein 1 63 2e-10
CBH2_SCHPO (O60108) CENP-B homolog protein 2 (CBHP-2) 54 1e-07
CBH1_SCHPO (O14423) CENP-B homolog protein 1 (CBHP-1) 39 0.003
CENB_HUMAN (P07199) Major centromere autoantigen B (Centromere p... 37 0.015
CENB_CRIGR (P48988) Major centromere autoantigen B (Centromere p... 36 0.034
CENB_MOUSE (P27790) Major centromere autoantigen B (Centromere p... 35 0.058
JRKL_HUMAN (Q9Y4A0) Jerky protein homolog like (HHMJG) 32 0.49
GL1_ARATH (P27900) Trichome differentiation protein GL1 (GLABROU... 31 1.1
RL4_RALSO (Q8XV13) 50S ribosomal protein L4 30 1.9
YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein 30 2.4
6PGD_CANAL (O13287) 6-phosphogluconate dehydrogenase, decarboxyl... 29 3.2
RPB2_CHLRE (Q8HTL7) DNA-directed RNA polymerase beta chain C-ter... 29 4.2
Y191_CLOAB (Q97MK5) Putative HTH-type transcriptional regulator ... 28 5.4
TRME_MYCPU (Q98RJ5) tRNA modification GTPase trmE 28 7.1
RP1_MOUSE (P56716) Oxygen-regulated protein 1 (Retinitis pigment... 28 7.1
OAS1_HUMAN (P00973) 2'-5'-oligoadenylate synthetase 1 (EC 2.7.7.... 28 7.1
MURC_XANCP (Q8PCJ9) UDP-N-acetylmuramate--L-alanine ligase (EC 6... 28 7.1
VP5_WTV (P12366) Outer coat protein P5 28 9.3
CAQ1_RABIT (P07221) Calsequestrin, skeletal muscle isoform precu... 28 9.3
CAQ1_HUMAN (P31415) Calsequestrin, skeletal muscle isoform precu... 28 9.3
>ABP1_SCHPO (P49777) ARS-binding protein 1
Length = 522
Score = 63.2 bits (152), Expect = 2e-10
Identities = 45/143 (31%), Positives = 69/143 (47%), Gaps = 15/143 (10%)
Query: 6 QPCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPGKINVLDAINLAIPTWTIDVRKETIA 65
QPC G+I A K YR+ + + ILE E+G++ NVL AI + WT D+ E I
Sbjct: 323 QPCSEGVIYALKACYRKHWVQYILEQNELGRNPYNTTNVLRAILWLVKAWTTDISPEIIE 382
Query: 66 NCFRHCKIRSASDVVGNLDES-----TFDEETQDLQTMINQCGYRNKMDIDNLMNYPGEN 120
N F + S V+G +ES DE L+ ++++ + M I++ ++ EN
Sbjct: 383 NAF------NLSGVLGLFNESAVTSRALDEMIHPLRELVSEFSVQAAMRIEDFISPSEEN 436
Query: 121 EACSEVQSLEDIVCTIIENNAED 143
V S EDI+ I +D
Sbjct: 437 ----IVDSSEDIINQIASQYMDD 455
>CBH2_SCHPO (O60108) CENP-B homolog protein 2 (CBHP-2)
Length = 514
Score = 53.9 bits (128), Expect = 1e-07
Identities = 40/147 (27%), Positives = 67/147 (45%), Gaps = 5/147 (3%)
Query: 2 TSKIQPCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPGKINVLDAINLAIPTWTIDVRK 61
T PC I+ AFK +YR+ + +LE +G++ +NVL A+ I +W +D
Sbjct: 318 TEIYHPCGQEIVYAFKSYYRKYWLNYMLEEIRLGKNPSKTMNVLKAVRWMIRSWNVDFEP 377
Query: 62 ETIANCF-RHCKIRSASDVVGNLDESTFDEETQDLQTMINQ-CGYRNKMDIDNLMNYPGE 119
I NCF R ++ + G E+ +LQ +I + G ++ I+N +N E
Sbjct: 378 SIIYNCFLRSGLFQNQQPLTGPSPETI--RIAVNLQELIGKYLGDKDIFQIENFINPIEE 435
Query: 120 NEACSEVQSLEDIVCTII-ENNAEDDE 145
N A + + + + E E DE
Sbjct: 436 NSADTNDDIVNQVAAQFLDEREFETDE 462
>CBH1_SCHPO (O14423) CENP-B homolog protein 1 (CBHP-1)
Length = 514
Score = 39.3 bits (90), Expect = 0.003
Identities = 28/140 (20%), Positives = 56/140 (40%), Gaps = 1/140 (0%)
Query: 7 PCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPGKINVLDAINLAIPTWTIDVRKETIAN 66
P + GI+ FK +YRR + + L + + +NVL AI + +W V ++TI
Sbjct: 323 PFEQGIVDTFKANYRRYWLQYSLNQINILRDPLKAVNVLKAIRWMLWSWNFGVSEKTIQA 382
Query: 67 CFRHCKIRSASDVVGNLDESTFDEETQDLQTMINQCGYRNKMDIDNLMNYPGENEACSEV 126
F + ++ + ++ +I+ G I+ + E+E
Sbjct: 383 SFLRSGLLGPHSEAEGQGMESYQKAIMEIGNLISSKGSEAVKQINEYIRPSEEDETKLHQ 442
Query: 127 QSLEDIVCTIIENNA-EDDE 145
+++ + +E E DE
Sbjct: 443 DAVDTVAAEFLEERRFESDE 462
>CENB_HUMAN (P07199) Major centromere autoantigen B (Centromere
protein B) (CENP-B)
Length = 599
Score = 37.0 bits (84), Expect = 0.015
Identities = 32/145 (22%), Positives = 59/145 (40%), Gaps = 6/145 (4%)
Query: 5 IQPCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPG-KINVLDAINLAIPTWTIDVRKET 63
+ P + G+++ K HYR+ K + E GQ G ++ + +A++ W V
Sbjct: 322 VHPLERGVVQQVKGHYRQAMLLKAMAALE-GQDPSGLQLGLTEALHFVAAAWQA-VEPSD 379
Query: 64 IANCFRHCKI---RSASDVVGNLDESTFDEETQDLQTMINQCGYRNKMDIDNLMNYPGEN 120
IA CFR +A+ E +EE ++ + G + + + GE
Sbjct: 380 IAACFREAGFGGGPNATITTSLKSEGEEEEEEEEEEEEEEGEGEEEEEEGEEEEEEGGEG 439
Query: 121 EACSEVQSLEDIVCTIIENNAEDDE 145
E E + +E+ + E+DE
Sbjct: 440 EELGEEEEVEEEGDVDSDEEEEEDE 464
>CENB_CRIGR (P48988) Major centromere autoantigen B (Centromere
protein B) (CENP-B)
Length = 606
Score = 35.8 bits (81), Expect = 0.034
Identities = 31/148 (20%), Positives = 59/148 (38%), Gaps = 9/148 (6%)
Query: 5 IQPCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPG-KINVLDAINLAIPTWTIDVRKET 63
+ P + G+++ K HYR+ K + E GQ G ++ +++A++ W V
Sbjct: 322 VHPLERGVVQQVKGHYRQAMLLKAMAALE-GQDPSGLQLGLVEALHFVAAAWQA-VEPAD 379
Query: 64 IANCFRHCKIRSASDVV------GNLDESTFDEETQDLQTMINQCGYRNKMDIDNLMNYP 117
IA CFR + +E +EE ++ + G + + +
Sbjct: 380 IATCFREAGFGGGLNATITTSFKSEGEEEEEEEEEEEEEEEEEGEGEEEEEEEEEGEEEG 439
Query: 118 GENEACSEVQSLEDIVCTIIENNAEDDE 145
GE E E + +E+ E E++E
Sbjct: 440 GEGEEVGEEEEVEEEGDESDEEEEEEEE 467
>CENB_MOUSE (P27790) Major centromere autoantigen B (Centromere
protein B) (CENP-B)
Length = 599
Score = 35.0 bits (79), Expect = 0.058
Identities = 19/66 (28%), Positives = 33/66 (49%), Gaps = 3/66 (4%)
Query: 5 IQPCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPG-KINVLDAINLAIPTWTIDVRKET 63
+ P + G+++ K HYR+ K + E GQ G ++ +++A++ W V
Sbjct: 322 VHPLERGVVQQVKGHYRQAMLLKAMAALE-GQDPSGLQLGLVEALHFVAAAWQA-VEPSD 379
Query: 64 IANCFR 69
IA CFR
Sbjct: 380 IATCFR 385
>JRKL_HUMAN (Q9Y4A0) Jerky protein homolog like (HHMJG)
Length = 442
Score = 32.0 bits (71), Expect = 0.49
Identities = 21/69 (30%), Positives = 34/69 (48%), Gaps = 1/69 (1%)
Query: 1 MTSKIQPCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPGKINVLDAINLAIPTWTIDVR 60
+ S IQP D G+I K +YR + LE +S K+ +LDA+ W + V+
Sbjct: 237 VASLIQPSDQGVIATMKRNYRAGLLQNNLEEGNDLKSFWKKLTLLDALYEIAMAWNL-VK 295
Query: 61 KETIANCFR 69
TI+ ++
Sbjct: 296 PVTISRAWK 304
>GL1_ARATH (P27900) Trichome differentiation protein GL1
(GLABROUS1 protein)
Length = 228
Score = 30.8 bits (68), Expect = 1.1
Identities = 22/80 (27%), Positives = 39/80 (48%), Gaps = 3/80 (3%)
Query: 18 MHYRRRFYRKILEGYEVGQSDPGKINVLD--AINLAIPTWTIDVRKETIANCFRHCKIRS 75
M RRR ++ E Y+ G + N+L +N W VRK + C + C++R
Sbjct: 1 MRIRRRDEKENQE-YKKGLWTVEEDNILMDYVLNHGTGQWNRIVRKTGLKRCGKSCRLRW 59
Query: 76 ASDVVGNLDESTFDEETQDL 95
+ + N+++ F E+ +DL
Sbjct: 60 MNYLSPNVNKGNFTEQEEDL 79
>RL4_RALSO (Q8XV13) 50S ribosomal protein L4
Length = 206
Score = 30.0 bits (66), Expect = 1.9
Identities = 18/73 (24%), Positives = 35/73 (47%), Gaps = 6/73 (8%)
Query: 16 FKMHYRRRFYRKILEGYEVGQSDPGKINVLDAINLAIPTWTIDVRKETIANCFRHCKIRS 75
F ++ YR + + G+INV+D++++ P + + +A+ FR + S
Sbjct: 97 FSQKVNKKMYRAGMRSIYSQLAREGRINVVDSLSVDAP------KTKLLADKFRAMGLDS 150
Query: 76 ASDVVGNLDESTF 88
+ NLDE+ F
Sbjct: 151 VLVITDNLDENLF 163
>YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein
Length = 1803
Score = 29.6 bits (65), Expect = 2.4
Identities = 23/91 (25%), Positives = 40/91 (43%), Gaps = 19/91 (20%)
Query: 39 PGKINVLDAINLAIPTWTIDVRKETIANCFRHCKIRSASDVVGNLDESTFDEETQDLQTM 98
P K ++ + N IP +T+D R N + + S +D DEE Q ++T+
Sbjct: 836 PSKNKIVTSDNYTIPNYTMDGRVRNTQNINKSHQFSSDND----------DEEDQ-IETV 884
Query: 99 INQCGYRNKMDIDNLMNYPGENEACSEVQSL 129
N C + L NY +N+ + ++ L
Sbjct: 885 TNLC--------EALENYEDDNKPITRLEDL 907
>6PGD_CANAL (O13287) 6-phosphogluconate dehydrogenase,
decarboxylating (EC 1.1.1.44)
Length = 517
Score = 29.3 bits (64), Expect = 3.2
Identities = 12/25 (48%), Positives = 18/25 (72%)
Query: 104 YRNKMDIDNLMNYPGENEACSEVQS 128
YR K D++NL+ YP N+A ++ QS
Sbjct: 407 YRKKPDLENLLLYPFFNDAITKAQS 431
>RPB2_CHLRE (Q8HTL7) DNA-directed RNA polymerase beta chain
C-terminal subunit (EC 2.7.7.6) (PEP) (Plastid-encoded
RNA polymerase beta C-terminal section) (RNA polymerase
beta subunit C-terminal section)
Length = 626
Score = 28.9 bits (63), Expect = 4.2
Identities = 23/105 (21%), Positives = 43/105 (40%), Gaps = 2/105 (1%)
Query: 13 IRAFKMHYRRRFYRKILEGYEVGQSDPGKINVL--DAINLAIPTWTIDVRKETIANCFRH 70
I++ M R+ ++ ++E E+ P VL + L + KET F+
Sbjct: 520 IKSDDMTGRQNLWKNLIENKEISLGSPESFKVLICELQALCLDIGLFRKNKETSLPYFKM 579
Query: 71 CKIRSASDVVGNLDESTFDEETQDLQTMINQCGYRNKMDIDNLMN 115
S ++V NL ++ + + N ++IDNL+N
Sbjct: 580 PGEESKTNVNANLTGVQSSKKLEANSQTVKNSSMPNLVEIDNLLN 624
>Y191_CLOAB (Q97MK5) Putative HTH-type transcriptional regulator
CAC0191
Length = 283
Score = 28.5 bits (62), Expect = 5.4
Identities = 23/67 (34%), Positives = 29/67 (42%), Gaps = 7/67 (10%)
Query: 68 FRHCKIRSASDVVGNLDES----TFDEETQDLQTMINQCGYRNKMDIDNLMNYPGEN--E 121
++ +I A D V N+D+ T DL T+I Y NK IDN M E E
Sbjct: 64 YKEFRIAIAKDTV-NVDKEENRYTDINPKDDLHTIIKNISYNNKKSIDNTMEVLSEELVE 122
Query: 122 ACSEVQS 128
SE S
Sbjct: 123 KASEAIS 129
>TRME_MYCPU (Q98RJ5) tRNA modification GTPase trmE
Length = 442
Score = 28.1 bits (61), Expect = 7.1
Identities = 32/150 (21%), Positives = 63/150 (41%), Gaps = 33/150 (22%)
Query: 4 KIQPCDAGIIRAFKMHYRRRFYRKILEGYEVGQSDPGKINVLDAINLAIPTWTIDVRKET 63
K++ + I +A ++ R R Y + VG+ + GK ++L+A+
Sbjct: 191 KLKDLEKEISKAVELSERSRIYFNEIPIAIVGRPNVGKSSLLNAL--------------- 235
Query: 64 IANCFRHCKIRSASDVVGNLDESTFDE-ETQDLQTMIN-----QCGYRNKMDIDNLMNYP 117
+ +V N++ +T D E + + IN G R+ +N++
Sbjct: 236 ---------LEEDKSIVTNIEGTTRDVVEARFVLNGINFLLKDTAGIRHS---ENVIEKI 283
Query: 118 GENEACSEVQSLEDIVCTIIENNAEDDERR 147
G ++ ++Q E I+ ++EN EDD R
Sbjct: 284 GIEKSFKQIQDSEIIIHLVLENQDEDDFER 313
>RP1_MOUSE (P56716) Oxygen-regulated protein 1 (Retinitis pigmentosa
RP1 protein homolog)
Length = 2095
Score = 28.1 bits (61), Expect = 7.1
Identities = 20/66 (30%), Positives = 31/66 (46%), Gaps = 12/66 (18%)
Query: 89 DEETQDLQTMINQCGYRNKMDIDNLMNYPGEN---------EACSEVQSLEDIVCTIIEN 139
+EET T +N+ G N + + +YPG+ EACS EDIV T +
Sbjct: 344 EEETVKWTTTVNRAGLSNNDEKNKKSSYPGKTDYGPSSLKLEACS---LPEDIVDTTQQG 400
Query: 140 NAEDDE 145
+ ++E
Sbjct: 401 SLTEEE 406
>OAS1_HUMAN (P00973) 2'-5'-oligoadenylate synthetase 1 (EC 2.7.7.-)
((2-5')oligo(A) synthetase 1) (2-5A synthetase 1)
(p46/p42 OAS) (E18/E16)
Length = 400
Score = 28.1 bits (61), Expect = 7.1
Identities = 18/71 (25%), Positives = 33/71 (46%), Gaps = 1/71 (1%)
Query: 67 CFRHCKIRSASDVVGNLDESTFDEETQDLQTMINQCGYRNKMDIDNLMNYPGENEACSEV 126
CF++ S + + ++ D+ET D + + GY + + + P +A S
Sbjct: 331 CFKNWDGSPVSSWILLAESNSADDETDDPRRY-QKYGYIGTHEYPHFSHRPSTLQAASTP 389
Query: 127 QSLEDIVCTII 137
Q+ ED CTI+
Sbjct: 390 QAEEDWTCTIL 400
>MURC_XANCP (Q8PCJ9) UDP-N-acetylmuramate--L-alanine ligase (EC
6.3.2.8) (UDP-N-acetylmuramoyl-L-alanine synthetase)
Length = 477
Score = 28.1 bits (61), Expect = 7.1
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Query: 21 RRRFYRKILEGYE--VGQSDPGKINVLDAINLAIPTWTIDVRKETIANCFRH 70
R RF ++ EG V + PG+ NVL+A+ A W + V +TIA +
Sbjct: 265 RMRFTLRLPEGTTTPVTLALPGRHNVLNALAAAAIGWQLGVAPDTIARALEN 316
>VP5_WTV (P12366) Outer coat protein P5
Length = 804
Score = 27.7 bits (60), Expect = 9.3
Identities = 14/45 (31%), Positives = 23/45 (51%)
Query: 52 IPTWTIDVRKETIANCFRHCKIRSASDVVGNLDESTFDEETQDLQ 96
IP ++ + TI N F+ CK RS V D+S D+ + ++
Sbjct: 8 IPNFSQTIDNRTIVNIFQSCKYRSQLSVCFLNDKSAADKFSNSMR 52
>CAQ1_RABIT (P07221) Calsequestrin, skeletal muscle isoform
precursor (Calsequestrin 1) (Aspartactin)
(Laminin-binding protein)
Length = 395
Score = 27.7 bits (60), Expect = 9.3
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Query: 90 EETQDLQTMINQCGYRNKMDIDNLMNYPGENEACSEVQSLEDIVCTIIEN--NAEDDE 145
E+T D+ Q G N D D++ + E + LED + ++E N EDD+
Sbjct: 328 EKTFDIDLSAPQIGVVNVTDADSVWMEMDDEEDLPSAEELEDWLEDVLEGEINTEDDD 385
>CAQ1_HUMAN (P31415) Calsequestrin, skeletal muscle isoform
precursor (Calsequestrin 1) (Calmitin)
Length = 390
Score = 27.7 bits (60), Expect = 9.3
Identities = 17/58 (29%), Positives = 27/58 (46%), Gaps = 2/58 (3%)
Query: 90 EETQDLQTMINQCGYRNKMDIDNLMNYPGENEACSEVQSLEDIVCTIIEN--NAEDDE 145
E+T D+ Q G N D D++ + E + LED + ++E N EDD+
Sbjct: 328 EKTFDIDLSAPQIGVVNVTDADSVWMEMDDEEDLPSAEELEDWLEDVLEGEINTEDDD 385
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,023,799
Number of Sequences: 164201
Number of extensions: 638859
Number of successful extensions: 1347
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1337
Number of HSP's gapped (non-prelim): 22
length of query: 147
length of database: 59,974,054
effective HSP length: 100
effective length of query: 47
effective length of database: 43,553,954
effective search space: 2047035838
effective search space used: 2047035838
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146790.4