BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146790.10 - phase: 0
(406 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
VIT_ICHUN (Q91062) Vitellogenin precursor (VTG) [Contains: Lipov... 35 0.34
YKK7_CAEEL (P34284) Hypothetical F-box/LRR-repeat protein C02F5.... 34 0.59
FXLK_RAT (Q9QZH7) F-box/LRR-repeat protein 20 (F-box and leucine... 34 0.77
FXLK_MOUSE (Q9CZV8) F-box/LRR-repeat protein 20 (F-box and leuci... 34 0.77
FXLK_HUMAN (Q96IG2) F-box/LRR-repeat protein 20 (F-box and leuci... 34 0.77
FL2_HUMAN (Q9UKC9) F-box/LRR-repeat protein 2 (F-box and leucine... 33 1.0
ACCD_ARATH (P56765) Acetyl-coenzyme A carboxylase carboxyl trans... 33 1.0
FL2_MOUSE (Q8BH16) F-box/LRR-repeat protein 2 (F-box and leucine... 32 2.2
C180_HUMAN (Q99467) CD180 antigen precursor (Lymphocyte antigen ... 32 2.2
DR20_ARATH (Q9SH22) Putative disease resistance protein At1g63360 32 2.9
YQOD_CAEEL (Q8IG36) Hypothetical RNA-binding protein EEED8.17 in... 32 3.8
SPS_VICFA (Q43876) Sucrose-phosphate synthase (EC 2.4.1.14) (UDP... 32 3.8
RF1_PYRHO (O59264) Peptide chain release factor subunit 1 (Trans... 32 3.8
RYR1_HUMAN (P21817) Ryanodine receptor 1 (Skeletal muscle-type r... 31 5.0
RNFA_HAEIN (P71395) Electron transport complex protein rnfA 31 5.0
LATA_LATMA (P23631) Alpha-latrotoxin precursor (Alpha-LTX) 31 5.0
FBX2_MOUSE (Q80UW2) F-box only protein 2 31 5.0
Y532_AQUAE (O66814) Hypothetical protein AQ_532 precursor 31 6.5
SYK_CAMJE (P41258) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--t... 31 6.5
YK86_YEAST (P36173) Hypothetical 69.0 kDa protein in SIR1 3'region 30 8.5
>VIT_ICHUN (Q91062) Vitellogenin precursor (VTG) [Contains:
Lipovitellin LV-1N; Lipovitellin LV-1C; Lipovitellin
LV-2]
Length = 1823
Score = 35.0 bits (79), Expect = 0.34
Identities = 33/156 (21%), Positives = 63/156 (40%), Gaps = 15/156 (9%)
Query: 104 RCHVCGNHKANLRDAEVYERRVEIDFESGMKIESGKKEQQFVNFVGRALLLTSISSMELE 163
RC C NLR VY + D SG I+S E+ + +S +++
Sbjct: 197 RCPTCQKMNKNLRSTAVYNYAI-FDEPSGYIIKSAHSEE-----------IQQLSVFDIK 244
Query: 164 RFSLLINNKRDISLQNTWISSILNRRVKILRIHSSFYQLPFSALTSHYLFNCTSLEELEL 223
+++I +++ + L+ + ++ + Y+ P SA+T T + LE
Sbjct: 245 EGNVVIESRQKLILEGIQSAPAASQAASLQNRGGLMYKFPSSAITKMSSLFVTKGKNLES 304
Query: 224 VLH--VSSTIKFPSISVH-FGHLKLLKLYGIFFKID 256
+H + ++ +SVH K L+L +D
Sbjct: 305 EIHTVLKHLVENNQLSVHEDAPAKFLRLTAFLRNVD 340
>YKK7_CAEEL (P34284) Hypothetical F-box/LRR-repeat protein C02F5.7
in chromosome III
Length = 461
Score = 34.3 bits (77), Expect = 0.59
Identities = 64/287 (22%), Positives = 107/287 (36%), Gaps = 43/287 (14%)
Query: 11 STQMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRWMNRWTSSITKLDLDD- 69
+T + ++ IN LP+ ++ +V SFL TK + R+ + + W SI LD +
Sbjct: 44 TTLSPAQVDNSLINRVLPKEVLLKVFSFLDTKALCRSAQVCRSW------SILALDGSNW 97
Query: 70 --IDLSYNYNPLCSYCEEICTDYCYDYDK-YKLTVCPRCH-----VCGNHKANLRDAEVY 121
+DL + + E C + K L C H + NL +Y
Sbjct: 98 QRVDLFTFQRDVKTAVVENLARRCGGFLKELSLKGCENVHDSALRTFTSRCPNLEHLSLY 157
Query: 122 ERRVEIDFESGMKIESGKKEQQFVNFVGRALLLTSISSMELERFSLLINNKRDISLQN-T 180
+ D E+ + +N+ L L + SS+ + + ++S N +
Sbjct: 158 RCKRVTD----ASCENLGRYCHKLNY----LNLENCSSITDRAMKYIGDGCPNLSYLNIS 209
Query: 181 WISSILNRRVKILRIHSSFYQLPFSALTSHYLFNCTSLEELELVLHVSSTIK-FPSISVH 239
W +I +R V+I+ L NC SL+ L L T F S+ H
Sbjct: 210 WCDAIQDRGVQII------------------LSNCKSLDTLILRGCEGLTENVFGSVEAH 251
Query: 240 FGHLKLLKLYGIFFKIDTSSDCLTLNLPLLRKFDIKNCNWSGGKDLI 286
G +K L L F D + + L + NCN + L+
Sbjct: 252 MGAIKKLNLLQCFQLTDITVQNIANGATALEYLCMSNCNQISDRSLV 298
>FXLK_RAT (Q9QZH7) F-box/LRR-repeat protein 20 (F-box and
leucine-rich repeat protein 20) (F-box/LRR-repeat
protein 2-like)
Length = 276
Score = 33.9 bits (76), Expect = 0.77
Identities = 16/42 (38%), Positives = 24/42 (57%)
Query: 13 QMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRW 54
+M S +E IN KLP+ L+ R+ SFL + R +S+ W
Sbjct: 14 EMFSNSDEAVINKKLPKELLLRIFSFLDVVTLCRCAQVSRAW 55
>FXLK_MOUSE (Q9CZV8) F-box/LRR-repeat protein 20 (F-box and
leucine-rich repeat protein 20) (F-box/LRR-repeat
protein 2-like)
Length = 436
Score = 33.9 bits (76), Expect = 0.77
Identities = 16/42 (38%), Positives = 24/42 (57%)
Query: 13 QMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRW 54
+M S +E IN KLP+ L+ R+ SFL + R +S+ W
Sbjct: 14 EMFSNSDEAVINKKLPKELLLRIFSFLDVVTLCRCAQVSRAW 55
>FXLK_HUMAN (Q96IG2) F-box/LRR-repeat protein 20 (F-box and
leucine-rich repeat protein 20) (F-box/LRR-repeat
protein 2-like)
Length = 436
Score = 33.9 bits (76), Expect = 0.77
Identities = 16/42 (38%), Positives = 24/42 (57%)
Query: 13 QMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRW 54
+M S +E IN KLP+ L+ R+ SFL + R +S+ W
Sbjct: 14 EMFSNSDEAVINKKLPKELLLRIFSFLDVVTLCRCAQVSRAW 55
>FL2_HUMAN (Q9UKC9) F-box/LRR-repeat protein 2 (F-box and
leucine-rich repeat protein 2) (F-box protein
FBL2/FBL3)
Length = 423
Score = 33.5 bits (75), Expect = 1.0
Identities = 15/36 (41%), Positives = 21/36 (57%)
Query: 19 EEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRW 54
+E IN KLP+ L+ R+ SFL + R +SK W
Sbjct: 7 DEGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAW 42
>ACCD_ARATH (P56765) Acetyl-coenzyme A carboxylase carboxyl
transferase subunit beta (EC 6.4.1.2) (ACCASE beta
chain)
Length = 488
Score = 33.5 bits (75), Expect = 1.0
Identities = 27/100 (27%), Positives = 41/100 (41%), Gaps = 19/100 (19%)
Query: 69 DIDLSYNYNPLCSYCEEICTDYCYD--YDKYKLTVCPRCHVCGNHKANLRDAEVYERRV- 125
D D++ NYN L C D CY Y K K+ VC +C H + +E E +
Sbjct: 217 DFDITQNYNQLWIQC-----DNCYGLMYKKVKMNVCEQC----GHYLKMSSSERIELSID 267
Query: 126 -------EIDFESGMKIESGKKEQQFVNFVGRALLLTSIS 158
+ D S I+ KE+ + N + A T ++
Sbjct: 268 PGTWNPMDEDMVSADPIKFHSKEEPYKNRIDSAQKTTGLT 307
>FL2_MOUSE (Q8BH16) F-box/LRR-repeat protein 2 (F-box and
leucine-rich repeat protein 2)
Length = 423
Score = 32.3 bits (72), Expect = 2.2
Identities = 14/36 (38%), Positives = 21/36 (57%)
Query: 19 EEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRW 54
++ IN KLP+ L+ R+ SFL + R +SK W
Sbjct: 7 DDGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAW 42
>C180_HUMAN (Q99467) CD180 antigen precursor (Lymphocyte antigen 64)
(Radioprotective 105 kDa protein)
Length = 661
Score = 32.3 bits (72), Expect = 2.2
Identities = 30/92 (32%), Positives = 48/92 (51%), Gaps = 10/92 (10%)
Query: 207 LTSHYLFNCTSLEELELV---LHVSSTIKFPSISVHFGHLKLLKLYGIFFKIDTSSDCLT 263
L S C LE L+L LH+++ + P ++HF L++L L F +DTS+ L
Sbjct: 411 LQSQAFKECPQLELLDLAFTRLHINAP-QSPFQNLHF--LQVLNLTYCF--LDTSNQHLL 465
Query: 264 LNLPLLRKFDIKNCNWSGGKDLIVEAPLLEIV 295
LP+LR ++K ++ G I + LL+ V
Sbjct: 466 AGLPVLRHLNLKGNHFQDG--TITKTNLLQTV 495
>DR20_ARATH (Q9SH22) Putative disease resistance protein At1g63360
Length = 884
Score = 32.0 bits (71), Expect = 2.9
Identities = 31/104 (29%), Positives = 54/104 (51%), Gaps = 10/104 (9%)
Query: 180 TWISSILNRRVKILRIHSSFYQLPFSALTSHYLFNCTSLEELELVLHVSSTIKFPSISVH 239
T ISS+ N +K+L++ S +LP+ T L +LE LE++ +S H
Sbjct: 632 TGISSLHN--LKVLKLFRS--RLPWDLNTVKEL---ETLEHLEILTTTIDPRAKQFLSSH 684
Query: 240 --FGHLKLLKLYGIFFK-IDTSSDCLTLNLPLLRKFDIKNCNWS 280
H +LL++YG ++ + L+++ LR+F IK+C+ S
Sbjct: 685 RLLSHSRLLEIYGSSVSSLNRHLESLSVSTDKLREFQIKSCSIS 728
>YQOD_CAEEL (Q8IG36) Hypothetical RNA-binding protein EEED8.17 in
chromosome II
Length = 310
Score = 31.6 bits (70), Expect = 3.8
Identities = 14/50 (28%), Positives = 29/50 (58%)
Query: 6 SKVHKSTQMASVIEEDTINSKLPESLINRVLSFLPTKDVVRTCVLSKRWM 55
S+ + +AS E+ +P+ ++ RV+SFLP + +R ++K++M
Sbjct: 224 SRASSTQSLASGSEQSFNLGNVPDKILRRVISFLPIHETIRLERVNKKFM 273
>SPS_VICFA (Q43876) Sucrose-phosphate synthase (EC 2.4.1.14)
(UDP-glucose-fructose-phosphate glucosyltransferase)
Length = 1059
Score = 31.6 bits (70), Expect = 3.8
Identities = 17/52 (32%), Positives = 26/52 (49%), Gaps = 6/52 (11%)
Query: 52 KRWMNRWTSSITKLDLDDIDLSYNYNPLCSYCEEICTDYCYDYDKYKLTVCP 103
++ + RW SSIT S N + S E++ TDYCY ++ K + P
Sbjct: 881 RKTLIRWASSITDKK------SENNEQIVSPAEQLSTDYCYAFNVRKAGMAP 926
>RF1_PYRHO (O59264) Peptide chain release factor subunit 1
(Translation termination factor aRF1)
Length = 417
Score = 31.6 bits (70), Expect = 3.8
Identities = 26/96 (27%), Positives = 47/96 (48%), Gaps = 16/96 (16%)
Query: 67 LDDIDLSYNYNPL-----CSYC--EEICTDYCYDYDKY--KLTVCPRCHVCGNHKANLRD 117
+D + +S YN + C++C EE+ T +Y+ Y K+T CP+C G+ +
Sbjct: 315 VDVLLISEGYNKVRVRVKCNHCGWEELKTMSEEEYEVYRKKITKCPKC---GSQNLTIEK 371
Query: 118 AEVYERRVEIDFESGMKIE----SGKKEQQFVNFVG 149
+V E +++ E+G +E ++ QQF G
Sbjct: 372 WDVAEELIKMAEEAGSDVEIISLDTEEGQQFYRAFG 407
>RYR1_HUMAN (P21817) Ryanodine receptor 1 (Skeletal muscle-type
ryanodine receptor) (RyR1) (RYR-1) (Skeletal muscle
calcium release channel)
Length = 5038
Score = 31.2 bits (69), Expect = 5.0
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
Query: 43 DVVRTCVLSKRWMNRWTSS-ITKLDLDDIDLSYNYNPLCSYCEE 85
D++ V S+R+++ T+S ++D + +N NP+CS CEE
Sbjct: 167 DIILVSVSSERYLHLSTASGELQVDASFMQTLWNMNPICSRCEE 210
>RNFA_HAEIN (P71395) Electron transport complex protein rnfA
Length = 192
Score = 31.2 bits (69), Expect = 5.0
Identities = 19/66 (28%), Positives = 34/66 (50%), Gaps = 4/66 (6%)
Query: 207 LTSHYLF---NCTSLEELELVLHVSSTIKFPSISVHFGHLKLLKLYGIFFKIDTSSDCLT 263
L HY+ N T L L +L ++ ++F ++++ L +L GIF + T ++C
Sbjct: 58 LVDHYILIPLNATFLRTLVFILVIAVVVQFTEMAINKTSPTLYRLLGIFLPLIT-TNCAV 116
Query: 264 LNLPLL 269
L + LL
Sbjct: 117 LGVALL 122
>LATA_LATMA (P23631) Alpha-latrotoxin precursor (Alpha-LTX)
Length = 1401
Score = 31.2 bits (69), Expect = 5.0
Identities = 34/117 (29%), Positives = 48/117 (40%), Gaps = 30/117 (25%)
Query: 251 IFFKIDTSSD--------CLTLNLPL---------LRKFDIKNCNWSGGKDLIVEAPLLE 293
+F KI SD C TL +P+ RKFD G + L
Sbjct: 395 LFSKISHWSDPVSVREKACPTLRMPVDQTRRNVLVFRKFDSSKPQLVGE----ITPYLSN 450
Query: 294 IVSIEQDIEFYNAASHDLHSQSIKFNALHLKQFTYSGYGTAQLIHLFDHGFLSFDSA 350
+ I++D+ YNAAS+ +A+ K+FT Y A + FDHG F +A
Sbjct: 451 FIDIDRDL--YNAASNP-------DSAVGFKEFTKLNYDGANIRATFDHGRTVFHAA 498
>FBX2_MOUSE (Q80UW2) F-box only protein 2
Length = 297
Score = 31.2 bits (69), Expect = 5.0
Identities = 19/59 (32%), Positives = 32/59 (54%), Gaps = 6/59 (10%)
Query: 2 EAGSSKVHKSTQMASVIEEDTINS-----KLPESLINRVLSFLPTKDVVRTC-VLSKRW 54
EAG+ + Q+ EE+ + +LPE L+ RVL+ LP ++V+ C ++ RW
Sbjct: 24 EAGAEASAEEEQLREAEEEEEAEAVEYLAELPEPLLLRVLAELPATELVQACRLVCLRW 82
>Y532_AQUAE (O66814) Hypothetical protein AQ_532 precursor
Length = 221
Score = 30.8 bits (68), Expect = 6.5
Identities = 16/37 (43%), Positives = 20/37 (53%)
Query: 186 LNRRVKILRIHSSFYQLPFSALTSHYLFNCTSLEELE 222
L RRV+ LR H Y PF + LFN SLE ++
Sbjct: 135 LKRRVEFLRNHEGKYCAPFDFGMAEALFNAVSLELMQ 171
>SYK_CAMJE (P41258) Lysyl-tRNA synthetase (EC 6.1.1.6) (Lysine--tRNA
ligase) (LysRS)
Length = 501
Score = 30.8 bits (68), Expect = 6.5
Identities = 20/82 (24%), Positives = 46/82 (55%), Gaps = 2/82 (2%)
Query: 95 DKYKLTVCPRCHVCGNHKANLRDAEVYERRV-EIDFESGMKIESGKKEQQFVNFVGRALL 153
++++L +C R G ++ N + YER + +I+ ++ E+ + ++ FVN +G +
Sbjct: 401 ERFELFICGRELANGFNELN-DPLDQYERFLKQIEAKNAGDEEACEMDEDFVNALGYGMP 459
Query: 154 LTSISSMELERFSLLINNKRDI 175
T+ + ++R +L+ NK+ I
Sbjct: 460 PTAGQGIGIDRLVMLLTNKKSI 481
>YK86_YEAST (P36173) Hypothetical 69.0 kDa protein in SIR1 3'region
Length = 615
Score = 30.4 bits (67), Expect = 8.5
Identities = 26/116 (22%), Positives = 56/116 (47%), Gaps = 5/116 (4%)
Query: 195 IHSSFYQLPFSALTSHYLFNCTSLEELELVLHVSSTIKFPSISVHFGHLKLLKLYGIFFK 254
+ S++ ++ + H L + +++ + V+ V S + + +S HFG L+L + IF+
Sbjct: 79 LRSTYTGYATNSYSEHSLLS--TVQVINAVVSVGSQVVYSRLSDHFGRLRLFLVATIFYI 136
Query: 255 IDTSSDCLTLNLPLLRKFDI-KNCNWSGGKDLIVEAPLLEIVSIEQDIEFYNAASH 309
+ T L + + NC + G +L++ L + S++ + FY AS+
Sbjct: 137 MGTIIQSQATRLTMYAAGSVFYNCGYV-GTNLLLTLILSDFSSLKWRM-FYQYASY 190
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.138 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 45,840,054
Number of Sequences: 164201
Number of extensions: 1891735
Number of successful extensions: 5555
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 5544
Number of HSP's gapped (non-prelim): 31
length of query: 406
length of database: 59,974,054
effective HSP length: 113
effective length of query: 293
effective length of database: 41,419,341
effective search space: 12135866913
effective search space used: 12135866913
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 67 (30.4 bits)
Medicago: description of AC146790.10