BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146777.10 - phase: 1 /pseudo
(406 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
VATA_MANSE (P31400) Vacuolar ATP synthase catalytic subunit A (E... 32 2.2
GATD_THEVO (Q979L8) Glutamyl-tRNA(Gln) amidotransferase subunit ... 32 2.2
BGL1_SACFI (P22506) Beta-glucosidase 1 precursor (EC 3.2.1.21) (... 32 2.9
OMPA_BUCAI (P57414) OmpA-like protein precursor 32 3.8
VAA2_DROME (Q27331) Vacuolar ATP synthase catalytic subunit A is... 31 5.0
VAA2_HUMAN (P38607) Vacuolar ATP synthase catalytic subunit A, o... 31 6.5
ARGB_CYAME (Q85FW5) Acetylglutamate kinase (EC 2.7.2.8) (NAG kin... 31 6.5
UNC7_CAEEL (Q03412) Innexin unc-7 (Uncoordinated protein 7) 30 8.5
UDPG_DICDI (P08800) UTP--glucose-1-phosphate uridylyltransferase... 30 8.5
>VATA_MANSE (P31400) Vacuolar ATP synthase catalytic subunit A (EC
3.6.3.14) (V-ATPase A subunit) (Vacuolar proton pump
alpha subunit) (V-ATPase 69 kDa subunit)
Length = 617
Score = 32.3 bits (72), Expect = 2.2
Identities = 29/104 (27%), Positives = 48/104 (45%), Gaps = 17/104 (16%)
Query: 94 EADAEEIIEIATKASVADQQMV------------VQENVHAQIKAFCTFMDAV-FLPNEK 140
E D EI+++ KAS+A+ + +Q+N ++ FC F V L N
Sbjct: 486 EEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLKNII 545
Query: 141 KVNDVS---FELSQQTKILPQHSDLRSANGKFVIHQLTILKLKD 181
D+S E + Q+ + +R A G V++QL+ +K KD
Sbjct: 546 SFYDMSRHAVESTAQSDNKVTWNVIRDAMGN-VLYQLSSMKFKD 588
>GATD_THEVO (Q979L8) Glutamyl-tRNA(Gln) amidotransferase subunit D
(EC 6.3.5.-) (Glu-ADT subunit D)
Length = 406
Score = 32.3 bits (72), Expect = 2.2
Identities = 29/128 (22%), Positives = 55/128 (42%), Gaps = 8/128 (6%)
Query: 97 AEEIIEIATKASVADQQMVVQENVHAQIKAFCTFMDAVFLPNEKKVNDVSFELSQQTKIL 156
A+ II + ++ S +EN+ I T + V + K ++D S L + +
Sbjct: 170 AQPIIFVGSQRSSDRPSSDTKENMEGAINFAATDLGEVGIAMHKGISDGSIVLHRAVRSR 229
Query: 157 PQHSDLRSANGKFVIHQLTILKLKDELGYTLNVKPSQISHKDAGQGLFLDGVVDVGAVVA 216
H+ R A + + L + YT +V+ K + L LD + + +++
Sbjct: 230 KMHTSRRDA-----FESIDTVHLAE---YTSSVRFFSDYRKAEEENLLLDHLDEKVSIIY 281
Query: 217 FYPGVVYS 224
F+PG+V S
Sbjct: 282 FHPGLVAS 289
>BGL1_SACFI (P22506) Beta-glucosidase 1 precursor (EC 3.2.1.21)
(Gentiobiase) (Cellobiase) (Beta-D-glucoside
glucohydrolase)
Length = 876
Score = 32.0 bits (71), Expect = 2.9
Identities = 37/146 (25%), Positives = 59/146 (40%), Gaps = 19/146 (13%)
Query: 257 GRGGDKKEL--WN-GRKMVDEKGSQVDNSDDVLERRNPLALAHFANHPSKGMLPNVMICP 313
G GD+K L WN G K+++ N+ V+ + FA+H PNV
Sbjct: 537 GNQGDRKNLTLWNNGDKLIETVAENCANTVVVVTSTGQINFEGFADH------PNVTAIV 590
Query: 314 YDFPLIENDMRAYIPNVLFGNAAEENTERFGSFWFKSRVPRNNESHVP--TTLKTVVLVA 371
+ PL + A I N+LFG A F + + ++ ++P T +
Sbjct: 591 WAGPLGDRSGTA-IANILFGKANPSGHLPF-------TIAKTDDDYIPIETYSPSSGEPE 642
Query: 372 TRALQDEELLLNYRLGNTKRCPEWYA 397
L + +LL++YR K YA
Sbjct: 643 DNHLVENDLLVDYRYFEEKNIEPRYA 668
>OMPA_BUCAI (P57414) OmpA-like protein precursor
Length = 349
Score = 31.6 bits (70), Expect = 3.8
Identities = 25/96 (26%), Positives = 37/96 (38%), Gaps = 9/96 (9%)
Query: 273 DEKGSQVDNSDDVLERRNPLALAHFANHPSKGMLPNVMICPYDFPLIENDMRAYIPNVLF 332
D SQV+N+D +E + P G+ PY IEND + P+++F
Sbjct: 45 DMNNSQVNNNDSSIENLSA---------PIVGLFLGYEFNPYFSLEIENDTNGFFPHLIF 95
Query: 333 GNAAEENTERFGSFWFKSRVPRNNESHVPTTLKTVV 368
E K P +E H+ T L +V
Sbjct: 96 QKNNEHIQSNSVQLATKLSYPITDEFHLYTKLGGIV 131
>VAA2_DROME (Q27331) Vacuolar ATP synthase catalytic subunit A
isoform 2 (EC 3.6.3.14) (V-ATPase A subunit 2) (Vacuolar
proton pump alpha subunit 2) (V-ATPase 69 kDa subunit 2)
Length = 614
Score = 31.2 bits (69), Expect = 5.0
Identities = 28/104 (26%), Positives = 49/104 (46%), Gaps = 17/104 (16%)
Query: 94 EADAEEIIEIATKASVADQQMV------------VQENVHAQIKAFCTFMDAV-FLPNEK 140
E D EI+++ KAS+A+ + +Q+N ++ FC F V L N
Sbjct: 483 EEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSSYDRFCPFYKTVGMLRNII 542
Query: 141 KVNDV---SFELSQQTKILPQHSDLRSANGKFVIHQLTILKLKD 181
D+ S E + Q++ + +R A G +++QL+ +K KD
Sbjct: 543 DFYDMARHSVESTAQSENKITWNVIREAMGN-IMYQLSSMKFKD 585
>VAA2_HUMAN (P38607) Vacuolar ATP synthase catalytic subunit A,
osteoclast isoform (EC 3.6.3.14) (V-ATPase A subunit 2)
(Vacuolar proton pump alpha subunit 2) (V-ATPase 69 kDa
subunit 2) (Isoform HO68)
Length = 615
Score = 30.8 bits (68), Expect = 6.5
Identities = 27/104 (25%), Positives = 49/104 (46%), Gaps = 17/104 (16%)
Query: 94 EADAEEIIEIATKASVADQQMV------------VQENVHAQIKAFCTFMDAV-FLPNEK 140
E D EI+++ KAS+A+ + +Q+N ++ FC F V L N
Sbjct: 484 EEDLSEIVQLVGKASLAETDKITLEVAKLLKDDFLQQNSYSPYDRFCPFYKTVGMLKNMI 543
Query: 141 KVNDVS---FELSQQTKILPQHSDLRSANGKFVIHQLTILKLKD 181
D+S E + Q++ + +R + G +++QL+ +K KD
Sbjct: 544 AFYDMSRHAVESTAQSENKITWNVIRDSMGN-ILYQLSSMKFKD 586
>ARGB_CYAME (Q85FW5) Acetylglutamate kinase (EC 2.7.2.8) (NAG
kinase) (AGK) (N-acetyl-L-glutamate
5-phosphotransferase)
Length = 290
Score = 30.8 bits (68), Expect = 6.5
Identities = 26/107 (24%), Positives = 43/107 (39%), Gaps = 10/107 (9%)
Query: 211 VGAVVAFYPGVVYSPAYYHHIPGYLDEQNPYLITRHDGNVIDAQLWGRGGDKKELWNGRK 270
VG + +P V+ H+IP + HDG + G+ +K
Sbjct: 151 VGEIEQIHPQVIDMLLEKHYIPVIAS-----VAASHDGTTYNLNADVVAGELAIKLKAKK 205
Query: 271 MV---DEKG--SQVDNSDDVLERRNPLALAHFANHPSKGMLPNVMIC 312
++ D KG + ++N + V+ N + AN S GM+P V C
Sbjct: 206 LIFLTDTKGILADINNENSVISTLNLKEAKNLANTISGGMIPKVNAC 252
>UNC7_CAEEL (Q03412) Innexin unc-7 (Uncoordinated protein 7)
Length = 522
Score = 30.4 bits (67), Expect = 8.5
Identities = 17/55 (30%), Positives = 23/55 (40%)
Query: 294 LAHFANHPSKGMLPNVMICPYDFPLIENDMRAYIPNVLFGNAAEENTERFGSFWF 348
L H GM P V +C ++ ++ N R + VL N E F FWF
Sbjct: 359 LMHAIEWEQTGMFPRVTLCDFEVRVLGNIHRHTVQCVLMINMFNEKIFLFLWFWF 413
>UDPG_DICDI (P08800) UTP--glucose-1-phosphate uridylyltransferase
(EC 2.7.7.9) (UDP-glucose pyrophosphorylase) (UDPGP)
(UGPase)
Length = 511
Score = 30.4 bits (67), Expect = 8.5
Identities = 23/99 (23%), Positives = 47/99 (47%), Gaps = 8/99 (8%)
Query: 111 DQQMVVQENVHAQIKAFCTFMDAVFLPNEKKVNDVSFELSQQTKILPQHSDLRSANGKFV 170
D+ Q+ HA I + D + +PN+ ++ D Q ++ S + +N +
Sbjct: 66 DELKAFQKQRHAYINKDQIYTDEIKIPNKTEMVDY-----HQLHLV---SPIDQSNASRL 117
Query: 171 IHQLTILKLKDELGYTLNVKPSQISHKDAGQGLFLDGVV 209
+++L ++KL LG ++ K ++ + + A FLD V
Sbjct: 118 LNKLVVIKLNGGLGNSMGCKTAKSTMEIAPGVTFLDMAV 156
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.140 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,471,378
Number of Sequences: 164201
Number of extensions: 2095696
Number of successful extensions: 4195
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 4193
Number of HSP's gapped (non-prelim): 9
length of query: 406
length of database: 59,974,054
effective HSP length: 113
effective length of query: 293
effective length of database: 41,419,341
effective search space: 12135866913
effective search space used: 12135866913
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 67 (30.4 bits)
Medicago: description of AC146777.10