BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146756.8 - phase: 0 /pseudo
(469 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 50 1e-05
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 46 2e-04
EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 43 0.002
YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni... 42 0.004
EX5A_BUCAI (P57530) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 40 0.017
YF19_METJA (Q58914) Hypothetical protein MJ1519 37 0.14
HELI_EHV1B (P28934) Probable helicase 36 0.19
LAMB_VIBCH (Q56652) Maltoporin precursor (Maltose-inducible pori... 36 0.24
S185_YEAST (P40856) SIT4-associating protein SAP185 35 0.41
HELI_HHV11 (P10189) Probable helicase 35 0.41
PRIA_BORBU (Q45032) Primosomal protein N' (Replication factor Y) 34 0.92
HELI_VZVD (P09303) Probable helicase 34 0.92
HELI_HHV2H (P28277) Probable helicase 34 0.92
EX5A_BUCAP (Q8K9A8) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 33 1.2
COBQ_ACIAD (Q43989) Cobyric acid synthase 33 2.1
DR17_ARATH (O64790) Putative disease resistance protein At1g61300 32 3.5
RRPO_SHVX (Q04575) RNA replication protein (194 kDa protein) (OR... 32 4.6
DNAA_SALTY (P35891) Chromosomal replication initiator protein dnaA 32 4.6
DNAA_SALTI (Q8Z2N6) Chromosomal replication initiator protein dnaA 32 4.6
DNAA_ECOLI (P03004) Chromosomal replication initiator protein dnaA 32 4.6
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 50.4 bits (119), Expect = 1e-05
Identities = 26/55 (47%), Positives = 40/55 (72%), Gaps = 1/55 (1%)
Query: 412 RRQFSIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKIL 466
R Q +++++AI+I+K+QGQ+LD V + L + VF Q YVALSR + EGL++L
Sbjct: 708 RSQIPLILAYAISIHKAQGQTLDRVKVDLGR-VFEKGQAYVALSRATTQEGLQVL 761
Score = 44.3 bits (103), Expect = 7e-04
Identities = 36/125 (28%), Positives = 65/125 (51%), Gaps = 12/125 (9%)
Query: 4 KFVFSVFMLDEQKNIYNRIMEVVTNEKGGVFFLYGYGGIGKTYMW----NILAASLRSRK 59
K V S+F+ DEQK RI+++V ++ +FF G G GK+ + +L + R +
Sbjct: 303 KSVPSIFLSDEQK----RILDMVVEQQHSIFFT-GSAGTGKSVLLRKIIEVLKSKYRKQS 357
Query: 60 DIVFNVATSGIVALLLPGGRTVHSRFKIPV--PTLQSSICDIEKGTELAELLKQTKLIIW 117
D V A++G+ A + GG T+HS + + ++ + I+K + +T+++I
Sbjct: 358 DRVAVTASTGLAACNI-GGVTLHSFAGVGLARESVDLLVSKIKKNKKCVNRWLRTRVLII 416
Query: 118 DEAPM 122
DE M
Sbjct: 417 DEVSM 421
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 45.8 bits (107), Expect = 2e-04
Identities = 26/58 (44%), Positives = 40/58 (68%), Gaps = 1/58 (1%)
Query: 412 RRQFSIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKILIHD 469
R Q ++++++++I+KSQGQ+L V + L + VF Q YVALSR S EGL++L D
Sbjct: 691 RVQLPLMLAWSLSIHKSQGQTLPKVKVDLRR-VFEKGQAYVALSRAVSREGLQVLNFD 747
Score = 33.1 bits (74), Expect = 1.6
Identities = 34/130 (26%), Positives = 58/130 (44%), Gaps = 19/130 (14%)
Query: 237 IEISNELLISNFTDPIEAIVQSTYSNLLENFQDGDFLRNRAILASTIQVVDDINNYVLNL 296
IE+ N + + N D E + L D + + A L ST V+ NN L+
Sbjct: 425 IEMLNRMRLGNIDDETER----EFKKLSRPLPDDEIIP--AELYSTRMEVERANNSRLSK 478
Query: 297 IPGEEKEYFSSDSIDRSKEDGNDAFEQVTPEFLNCLKTYGLPNHSIILKVGTPIMLLRNL 356
+PG+ + ++ID + + E++ FL + + LKVG +M+++NL
Sbjct: 479 LPGQVHIF---NAIDGGALEDEELKERLLQNFLAPKELH--------LKVGAQVMMVKNL 527
Query: 357 DQAEGLCNGT 366
D L NG+
Sbjct: 528 DAT--LVNGS 535
>EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5) (Exodeoxyribonuclease V 67 kDa polypeptide)
Length = 608
Score = 43.1 bits (100), Expect = 0.002
Identities = 35/120 (29%), Positives = 55/120 (45%), Gaps = 32/120 (26%)
Query: 347 GTPIMLLRNLDQAEGLCNGTRLTITRLANHVIEVKIITEKNIGNQVYIPGI-----SMSP 401
G P+M+ RN D A GL NG ++ I ++ G +V+ S+ P
Sbjct: 479 GRPVMIARN-DSALGLFNG-------------DIGIALDRGQGTRVWFAMPDGNIKSVQP 524
Query: 402 SKLPWPFKLVRRQFSIVVSFAITINKSQGQSLDHVGLYLPKD---VFSHEQLYVALSRVK 458
S+LP ++A+T++KSQG DH L LP V + E +Y A++R +
Sbjct: 525 SRLP----------EHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRAR 574
>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
region
Length = 723
Score = 41.6 bits (96), Expect = 0.004
Identities = 31/108 (28%), Positives = 57/108 (52%), Gaps = 13/108 (12%)
Query: 373 LANHVIEVKIITEK------NIGNQVYIPGISMSPSKLPWPFK-----LVRRQFSIVVSF 421
L N+ +++ EK +G YI + M P + P L R Q +++ +
Sbjct: 597 LINYAVKISPHKEKFPYVRWTVGKNKYIHEL-MVPERFPIDIPRENVGLERTQIPLMLCW 655
Query: 422 AITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKILIHD 469
A++I+K+QGQ++ + + L + +F Q+YVALSR + + L++L D
Sbjct: 656 ALSIHKAQGQTIQRLKVDL-RRIFEAGQVYVALSRAVTMDTLQVLNFD 702
>EX5A_BUCAI (P57530) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5)
Length = 602
Score = 39.7 bits (91), Expect = 0.017
Identities = 31/115 (26%), Positives = 57/115 (48%), Gaps = 23/115 (20%)
Query: 346 VGTPIMLLRNLDQAEGLCNGTRLTITRL-ANHVIEVKIITEKNIGNQVYIPGISMSPSKL 404
+G PIM++ N ++A + NG + IT + N +++V + E N N +
Sbjct: 480 IGKPIMIINN-NRALNVSNGN-IGITNINKNGILQVSFLKENNTINNI------------ 525
Query: 405 PWPFKLVRRQFSIVVSFAITINKSQGQSLDHVGLYLP---KDVFSHEQLYVALSR 456
P K++R + ++AIT++KSQG + L LP + + + LY ++R
Sbjct: 526 --PVKILR---NYKTAWAITVHKSQGSEFMNTALILPNFNSHILNKDTLYTGITR 575
>YF19_METJA (Q58914) Hypothetical protein MJ1519
Length = 1175
Score = 36.6 bits (83), Expect = 0.14
Identities = 19/42 (45%), Positives = 28/42 (66%), Gaps = 3/42 (7%)
Query: 420 SFAITINKSQGQSLDHVGLYLPKDV---FSHEQLYVALSRVK 458
++AITI+KSQG ++V L +PK + S E LY A++R K
Sbjct: 960 AYAITIHKSQGSGFENVILIIPKGLNKFVSKEMLYTAITRAK 1001
>HELI_EHV1B (P28934) Probable helicase
Length = 881
Score = 36.2 bits (82), Expect = 0.19
Identities = 18/51 (35%), Positives = 30/51 (58%)
Query: 415 FSIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKI 465
+ + A+TI +SQG SLD V + P++ +YVA+SR S+ L++
Sbjct: 799 YGLSSKLAMTIARSQGLSLDKVAICFPRNNLRINSVYVAMSRTVSSRFLRM 849
>LAMB_VIBCH (Q56652) Maltoporin precursor (Maltose-inducible porin)
(Outer membrane protein S)
Length = 412
Score = 35.8 bits (81), Expect = 0.24
Identities = 22/72 (30%), Positives = 36/72 (49%), Gaps = 7/72 (9%)
Query: 221 KVGDGKLGEENDGYNDIEISNELLISNFTDPIEAIVQSTYSNLLENFQDGDF------LR 274
K G G+LG END Y + + EL T +E+++ S ++DGDF ++
Sbjct: 47 KAGTGRLGNENDNYYEFGFAEELKTGEQTWKVESMIAQGNSG-ANGWEDGDFNVAQFNVQ 105
Query: 275 NRAILASTIQVV 286
+ +LAS + V
Sbjct: 106 AKGLLASDQEAV 117
>S185_YEAST (P40856) SIT4-associating protein SAP185
Length = 1058
Score = 35.0 bits (79), Expect = 0.41
Identities = 29/90 (32%), Positives = 44/90 (48%), Gaps = 12/90 (13%)
Query: 231 NDGYNDIEISNELLISNFTDPIEAIVQSTYSNLLENFQDGDFLRNRAILASTIQVVDDIN 290
ND +D E+ EL+ SNF + +V Y +L D++RN IL S I D +
Sbjct: 84 NDLLDDEELYTELMCSNF----KLLVYLKYPEVLSKLI--DYVRNSTILESNI---DRVT 134
Query: 291 NYVLNLIPGEEK---EYFSSDSIDRSKEDG 317
+ +L+ GE+K E F + D+ DG
Sbjct: 135 SEDRDLVRGEDKDTTEDFENAKADKKNIDG 164
>HELI_HHV11 (P10189) Probable helicase
Length = 882
Score = 35.0 bits (79), Expect = 0.41
Identities = 19/55 (34%), Positives = 28/55 (50%)
Query: 411 VRRQFSIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKI 465
+ + I A+TI +SQG SLD V + YVA+SR S+E L++
Sbjct: 796 INADYGISSKLAMTITRSQGLSLDKVAICFTPGNLRLNSAYVAMSRTTSSEFLRM 850
>PRIA_BORBU (Q45032) Primosomal protein N' (Replication factor Y)
Length = 660
Score = 33.9 bits (76), Expect = 0.92
Identities = 16/33 (48%), Positives = 23/33 (69%), Gaps = 3/33 (9%)
Query: 13 DEQKNIYNRIMEVVTNEKGGVFFLYGYGGIGKT 45
+EQ+NIY E++ +EK VF+L+G G GKT
Sbjct: 136 NEQQNIYK---EIIGSEKTNVFYLFGIPGSGKT 165
>HELI_VZVD (P09303) Probable helicase
Length = 881
Score = 33.9 bits (76), Expect = 0.92
Identities = 18/51 (35%), Positives = 28/51 (54%)
Query: 415 FSIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGLKI 465
+ + A+TI +SQG SL+ V + D +YVA+SR S+ LK+
Sbjct: 799 YGLSSKLAMTIARSQGLSLEKVAICFTADKLRLNSVYVAMSRTVSSRFLKM 849
>HELI_HHV2H (P28277) Probable helicase
Length = 881
Score = 33.9 bits (76), Expect = 0.92
Identities = 19/53 (35%), Positives = 26/53 (48%)
Query: 411 VRRQFSIVVSFAITINKSQGQSLDHVGLYLPKDVFSHEQLYVALSRVKSNEGL 463
+ + I A+TI +SQG SLD V + YVA+SR S+E L
Sbjct: 795 INADYGISSKLAMTITRSQGLSLDKVAICFTPGNLRLNSAYVAMSRTTSSEFL 847
>EX5A_BUCAP (Q8K9A8) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5)
Length = 602
Score = 33.5 bits (75), Expect = 1.2
Identities = 26/117 (22%), Positives = 57/117 (48%), Gaps = 23/117 (19%)
Query: 346 VGTPIMLLRNLDQAEGLCNGTRLTITRLAN-HVIEVKIITEKNIGNQVYIPGISMSPSKL 404
+G PI++ N +Q G+ NG + IT L+ + ++V + + N +
Sbjct: 486 IGKPIIITEN-NQCLGIFNG-EIGITNLSQKNTLQVSFLKKDNFIENI------------ 531
Query: 405 PWPFKLVRRQFSIVVSFAITINKSQGQSLDHVGLYLPK---DVFSHEQLYVALSRVK 458
P ++++ + S++IT++K+QG +++ L LP + + LY ++R +
Sbjct: 532 --PIEILK---NYKTSWSITVHKAQGSEFNNISLILPNKNLKILKKDILYTGITRTR 583
>COBQ_ACIAD (Q43989) Cobyric acid synthase
Length = 465
Score = 32.7 bits (73), Expect = 2.1
Identities = 27/74 (36%), Positives = 35/74 (46%), Gaps = 7/74 (9%)
Query: 16 KNIYNRIMEVVTNEKGGVFFLYGYGGIGKTYMWNILAASLRSRKDIVFNVATSGIVALLL 75
K+ +I+ V EKGGVF + TY N+L A LRS V G ++L
Sbjct: 153 KHYQTKIILVADIEKGGVF-----ASVWGTY--NLLPADLRSNVIGVIINKFRGDLSLFD 205
Query: 76 PGGRTVHSRFKIPV 89
G R + FKIPV
Sbjct: 206 EGIRIIEQAFKIPV 219
>DR17_ARATH (O64790) Putative disease resistance protein At1g61300
Length = 766
Score = 32.0 bits (71), Expect = 3.5
Identities = 19/53 (35%), Positives = 31/53 (57%), Gaps = 7/53 (13%)
Query: 19 YNRIMEVVTNEKGGVFFLYGYGGIGKTYMWNIL---AASLRSRKDIVFNVATS 68
+NR+ME ++ G+ L+G GG+GKT ++ + A + SR DIV + S
Sbjct: 53 WNRLME----DRVGIMGLHGMGGVGKTTLFKKIHNKFAKMSSRFDIVIWIVVS 101
>RRPO_SHVX (Q04575) RNA replication protein (194 kDa protein) (ORF 1)
[Contains: RNA-directed RNA polymerase (EC 2.7.7.48);
Probable helicase]
Length = 1718
Score = 31.6 bits (70), Expect = 4.6
Identities = 29/104 (27%), Positives = 46/104 (43%), Gaps = 5/104 (4%)
Query: 359 AEGLCNGTRLTITRLANHVIEVKIITEKNIGNQVYIPGISMSPSKLPWPFKLVRRQFSIV 418
A+ N T RLAN +K+ E+ +G V I + + P + + +
Sbjct: 1038 ADYYLNATHRQPRRLAN---PIKVHAERQLGGAVLKANIVPDLAMVLVPAFRSQSLLTDL 1094
Query: 419 VSFAITINKSQGQSLDHVGLYLPKD--VFSHEQLYVALSRVKSN 460
A+T QG +L+H+ + L KD + S E LY A SR +
Sbjct: 1095 GRHAMTYAGCQGLTLNHLTIILDKDTPLCSDEVLYTAFSRASES 1138
>DNAA_SALTY (P35891) Chromosomal replication initiator protein dnaA
Length = 466
Score = 31.6 bits (70), Expect = 4.6
Identities = 16/62 (25%), Positives = 30/62 (47%), Gaps = 3/62 (4%)
Query: 1 MQIKFVFSVFMLDEQKNIYNRIMEVVTNEKGGVF---FLYGYGGIGKTYMWNILAASLRS 57
+ +K F F+ + + V + GG + FLYG G+GKT++ + + + +
Sbjct: 131 VNVKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHAVGNGIMA 190
Query: 58 RK 59
RK
Sbjct: 191 RK 192
>DNAA_SALTI (Q8Z2N6) Chromosomal replication initiator protein dnaA
Length = 466
Score = 31.6 bits (70), Expect = 4.6
Identities = 16/62 (25%), Positives = 30/62 (47%), Gaps = 3/62 (4%)
Query: 1 MQIKFVFSVFMLDEQKNIYNRIMEVVTNEKGGVF---FLYGYGGIGKTYMWNILAASLRS 57
+ +K F F+ + + V + GG + FLYG G+GKT++ + + + +
Sbjct: 131 VNVKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHAVGNGIMA 190
Query: 58 RK 59
RK
Sbjct: 191 RK 192
>DNAA_ECOLI (P03004) Chromosomal replication initiator protein dnaA
Length = 467
Score = 31.6 bits (70), Expect = 4.6
Identities = 16/62 (25%), Positives = 30/62 (47%), Gaps = 3/62 (4%)
Query: 1 MQIKFVFSVFMLDEQKNIYNRIMEVVTNEKGGVF---FLYGYGGIGKTYMWNILAASLRS 57
+ +K F F+ + + V + GG + FLYG G+GKT++ + + + +
Sbjct: 132 VNVKHTFDNFVEGKSNQLARAAARQVADNPGGAYNPLFLYGGTGLGKTHLLHAVGNGIMA 191
Query: 58 RK 59
RK
Sbjct: 192 RK 193
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.328 0.144 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 50,441,738
Number of Sequences: 164201
Number of extensions: 2080260
Number of successful extensions: 7027
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 20
Number of HSP's that attempted gapping in prelim test: 7016
Number of HSP's gapped (non-prelim): 28
length of query: 469
length of database: 59,974,054
effective HSP length: 114
effective length of query: 355
effective length of database: 41,255,140
effective search space: 14645574700
effective search space used: 14645574700
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 68 (30.8 bits)
Medicago: description of AC146756.8