BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146743.2 - phase: 0
(68 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PIF1_YEAST (P07271) DNA repair and recombination protein PIF1, m... 42 3e-04
PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1, m... 42 4e-04
YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergeni... 35 0.036
HELI_VZVD (P09303) Probable helicase 33 0.10
EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 33 0.10
HELI_EHV1B (P28934) Probable helicase 33 0.14
HELI_HHV11 (P10189) Probable helicase 32 0.23
HELI_HHV2H (P28277) Probable helicase 31 0.68
HELI_HCMVA (P16736) Probable helicase 30 0.88
HELI_SHV21 (Q01014) Probable helicase 30 1.2
HELI_HHV6Z (P52450) Probable helicase 29 2.6
HELI_HHV6U (P52356) Probable helicase 29 2.6
GLMS_MYCSM (O68956) Glucosamine--fructose-6-phosphate aminotrans... 28 3.4
EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC 3.1.1... 28 3.4
HELI_HHV7J (P52357) Probable helicase 28 4.4
OPGG_PSEPK (Q88D03) Glucans biosynthesis protein G precursor 28 5.7
FBP1_DROME (Q04691) Fat-body protein-1 precursor (P1 protein) 27 7.5
>PIF1_YEAST (P07271) DNA repair and recombination protein PIF1,
mitochondrial precursor
Length = 857
Score = 42.0 bits (97), Expect = 3e-04
Identities = 23/44 (52%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
Query: 1 MTINKSQGQSLEHVGVYLPSPIFSHGQLYVAISRVTSRGGLKIL 44
++I+KSQGQ+L V V L +F GQ YVA+SR SR GL++L
Sbjct: 702 LSIHKSQGQTLPKVKVDLRR-VFEKGQAYVALSRAVSREGLQVL 744
>PIF1_SCHPO (Q9UUA2) DNA repair and recombination protein pif1,
mitochondrial precursor
Length = 805
Score = 41.6 bits (96), Expect = 4e-04
Identities = 21/44 (47%), Positives = 33/44 (74%), Gaps = 1/44 (2%)
Query: 1 MTINKSQGQSLEHVGVYLPSPIFSHGQLYVAISRVTSRGGLKIL 44
++I+K+QGQ+L+ V V L +F GQ YVA+SR T++ GL++L
Sbjct: 719 ISIHKAQGQTLDRVKVDL-GRVFEKGQAYVALSRATTQEGLQVL 761
>YHJ1_YEAST (P38766) Hypothetical helicase in SLT2-PUT2 intergenic
region
Length = 723
Score = 35.0 bits (79), Expect = 0.036
Identities = 18/44 (40%), Positives = 31/44 (69%), Gaps = 1/44 (2%)
Query: 1 MTINKSQGQSLEHVGVYLPSPIFSHGQLYVAISRVTSRGGLKIL 44
++I+K+QGQ+++ + V L IF GQ+YVA+SR + L++L
Sbjct: 657 LSIHKAQGQTIQRLKVDLRR-IFEAGQVYVALSRAVTMDTLQVL 699
>HELI_VZVD (P09303) Probable helicase
Length = 881
Score = 33.5 bits (75), Expect = 0.10
Identities = 19/46 (41%), Positives = 26/46 (56%)
Query: 1 MTINKSQGQSLEHVGVYLPSPIFSHGQLYVAISRVTSRGGLKILIN 46
MTI +SQG SLE V + + +YVA+SR S LK+ +N
Sbjct: 807 MTIARSQGLSLEKVAICFTADKLRLNSVYVAMSRTVSSRFLKMNLN 852
>EX5A_ECOLI (P04993) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5) (Exodeoxyribonuclease V 67 kDa polypeptide)
Length = 608
Score = 33.5 bits (75), Expect = 0.10
Identities = 15/41 (36%), Positives = 24/41 (57%), Gaps = 3/41 (7%)
Query: 1 MTINKSQGQSLEHVGVYLPS---PIFSHGQLYVAISRVTSR 38
MT++KSQG +H + LPS P+ + +Y A++R R
Sbjct: 536 MTVHKSQGSEFDHAALILPSQRTPVVTRELVYTAVTRARRR 576
>HELI_EHV1B (P28934) Probable helicase
Length = 881
Score = 33.1 bits (74), Expect = 0.14
Identities = 18/46 (39%), Positives = 26/46 (56%)
Query: 1 MTINKSQGQSLEHVGVYLPSPIFSHGQLYVAISRVTSRGGLKILIN 46
MTI +SQG SL+ V + P +YVA+SR S L++ +N
Sbjct: 807 MTIARSQGLSLDKVAICFPRNNLRINSVYVAMSRTVSSRFLRMNLN 852
>HELI_HHV11 (P10189) Probable helicase
Length = 882
Score = 32.3 bits (72), Expect = 0.23
Identities = 22/59 (37%), Positives = 30/59 (50%), Gaps = 2/59 (3%)
Query: 1 MTINKSQGQSLEHVGVYLPSPIFSHGQLYVAISRVTSRGGLKILIND--DDDDNIDVAS 57
MTI +SQG SL+ V + YVA+SR TS L++ +N + D DV S
Sbjct: 808 MTITRSQGLSLDKVAICFTPGNLRLNSAYVAMSRTTSSEFLRMNLNPLRERHDRDDVIS 866
>HELI_HHV2H (P28277) Probable helicase
Length = 881
Score = 30.8 bits (68), Expect = 0.68
Identities = 18/46 (39%), Positives = 24/46 (52%)
Query: 1 MTINKSQGQSLEHVGVYLPSPIFSHGQLYVAISRVTSRGGLKILIN 46
MTI +SQG SL+ V + YVA+SR TS L + +N
Sbjct: 807 MTITRSQGLSLDKVAICFTPGNLRLNSAYVAMSRTTSSEFLHMNLN 852
>HELI_HCMVA (P16736) Probable helicase
Length = 956
Score = 30.4 bits (67), Expect = 0.88
Identities = 23/50 (46%), Positives = 29/50 (58%), Gaps = 6/50 (12%)
Query: 1 MTINKSQGQSLEHVGV----YLPSPIFSHGQLYVAISRVTSRGGLKILIN 46
MTI KSQG SLE V V + + SH +YVA+SRVT L + +N
Sbjct: 880 MTIAKSQGLSLEKVAVDFGDHPKNLKMSH--IYVAMSRVTDPEHLMMNVN 927
>HELI_SHV21 (Q01014) Probable helicase
Length = 781
Score = 30.0 bits (66), Expect = 1.2
Identities = 21/55 (38%), Positives = 29/55 (52%), Gaps = 8/55 (14%)
Query: 1 MTINKSQGQSLEHVGV-YLPSPIFSHGQLYVAISRVTSR-------GGLKILIND 47
MTI K+QG SL+ V + + +YVAISR T+ LK+L+ND
Sbjct: 705 MTIVKAQGTSLDKVAICFSNHKKIKVSHIYVAISRATNPNHIVMDCNPLKLLVND 759
>HELI_HHV6Z (P52450) Probable helicase
Length = 824
Score = 28.9 bits (63), Expect = 2.6
Identities = 18/48 (37%), Positives = 25/48 (51%), Gaps = 2/48 (4%)
Query: 1 MTINKSQGQSLEHVGVYLPS--PIFSHGQLYVAISRVTSRGGLKILIN 46
MTI KSQG S++ V + S +YV +SRVT L + +N
Sbjct: 748 MTITKSQGLSIQKVAIDFGSDPKNLKLSSIYVGMSRVTDPNNLIMNVN 795
>HELI_HHV6U (P52356) Probable helicase
Length = 824
Score = 28.9 bits (63), Expect = 2.6
Identities = 18/48 (37%), Positives = 25/48 (51%), Gaps = 2/48 (4%)
Query: 1 MTINKSQGQSLEHVGVYLPS--PIFSHGQLYVAISRVTSRGGLKILIN 46
MTI KSQG S++ V + S +YV +SRVT L + +N
Sbjct: 748 MTITKSQGLSIQKVAIDFGSDPKNLKLSSIYVGMSRVTDPNNLIMNVN 795
>GLMS_MYCSM (O68956) Glucosamine--fructose-6-phosphate
aminotransferase [isomerizing] (EC 2.6.1.16)
(Hexosephosphate aminotransferase)
(D-fructose-6-phosphate amidotransferase) (GFAT)
(L-glutamine-D-fructose-6-phosphate amidotransferase)
(Glucosamine-6-ph
Length = 627
Score = 28.5 bits (62), Expect = 3.4
Identities = 13/41 (31%), Positives = 23/41 (55%), Gaps = 3/41 (7%)
Query: 16 VYLPSP---IFSHGQLYVAISRVTSRGGLKILINDDDDDNI 53
V +PSP H +L I + +RG + ++I ++DDD +
Sbjct: 533 VVMPSPKNAAMLHAKLLSNIREIQARGAVTVVIAEEDDDTV 573
>EX5A_HAEIN (P45158) Exodeoxyribonuclease V alpha chain (EC
3.1.11.5)
Length = 640
Score = 28.5 bits (62), Expect = 3.4
Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Query: 1 MTINKSQGQSLEHVGVYLP---SPIFSHGQLYVAISR 34
MTI+KSQG +H + LP +P+ S ++ ++R
Sbjct: 568 MTIHKSQGSEFKHTVMVLPTEVNPVLSRELVFTGVTR 604
>HELI_HHV7J (P52357) Probable helicase
Length = 820
Score = 28.1 bits (61), Expect = 4.4
Identities = 21/66 (31%), Positives = 36/66 (53%), Gaps = 7/66 (10%)
Query: 1 MTINKSQGQSLEHVGVYLPS--PIFSHGQLYVAISRVTSRGGLKILIN----DDDDDNID 54
MT+ KSQG S++ V + + +YV +SRV L + +N + ++DNI
Sbjct: 744 MTVTKSQGLSIQRVALDFGNDPKNLKLSSIYVGMSRVVDPNNLIMNLNPLRLNYENDNI- 802
Query: 55 VASNVV 60
+AS++V
Sbjct: 803 IASHIV 808
>OPGG_PSEPK (Q88D03) Glucans biosynthesis protein G precursor
Length = 559
Score = 27.7 bits (60), Expect = 5.7
Identities = 13/45 (28%), Positives = 20/45 (43%)
Query: 19 PSPIFSHGQLYVAISRVTSRGGLKILINDDDDDNIDVASNVVYRE 63
P P H +Y + S G K+ + +D +DV S V R+
Sbjct: 191 PKPADKHLVIYALLDSPRSTGAYKLTLRPGNDTVVDVQSRVFLRD 235
>FBP1_DROME (Q04691) Fat-body protein-1 precursor (P1 protein)
Length = 1030
Score = 27.3 bits (59), Expect = 7.5
Identities = 12/22 (54%), Positives = 16/22 (72%)
Query: 46 NDDDDDNIDVASNVVYREVFRN 67
NDDDDD+ D NVV+R+ R+
Sbjct: 580 NDDDDDDNDDDVNVVHRQGLRS 601
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.138 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,522,433
Number of Sequences: 164201
Number of extensions: 245097
Number of successful extensions: 620
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 608
Number of HSP's gapped (non-prelim): 18
length of query: 68
length of database: 59,974,054
effective HSP length: 44
effective length of query: 24
effective length of database: 52,749,210
effective search space: 1265981040
effective search space used: 1265981040
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146743.2