BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146743.12 + phase: 0
(139 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NL22_PARJU (O04403) Probable nonspecific lipid-transfer protein ... 44 1e-04
UGP5_ARATH (Q9C7F7) Uncharacterized GPI-anchored protein At1g279... 41 7e-04
NL21_PARJU (P55958) Probable nonspecific lipid-transfer protein ... 39 0.003
MEN8_SILLA (O24356) MEN-8 protein precursor 36 0.022
NLT1_TOBAC (Q42952) Nonspecific lipid-transfer protein 1 precurs... 34 0.11
NLT2_ORYSA (Q42978) Nonspecific lipid-transfer protein 2 precurs... 33 0.19
NLT2_SORBI (Q43194) Nonspecific lipid-transfer protein 2 precurs... 33 0.25
NLT2_LYCES (P27056) Nonspecific lipid-transfer protein 2 precurs... 33 0.25
PRF1_LYCES (Q00451) 36.4 kDa proline-rich protein 32 0.32
NL41_HORVU (Q43767) Nonspecific lipid-transfer protein 4.1 precu... 32 0.32
MC1_PINRA (O24493) Male-cone protein 1 precursor (PRMC1) 32 0.32
CCDP_MAIZE (Q01595) Cortical cell delineating protein precursor ... 32 0.32
NL43_HORVU (Q42842) Nonspecific lipid-transfer protein 4.3 precu... 32 0.42
NLT2_ARATH (Q9S7I3) Nonspecific lipid-transfer protein 2 precurs... 32 0.55
NL42_HORVU (Q43875) Nonspecific lipid-transfer protein 4.2 precu... 31 0.72
A9_BRANA (Q05772) Tapetum-specific protein A9 precursor 31 0.72
LEU3_BACCR (Q81G11) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 31 0.93
LEU3_BACAN (Q81T67) 3-isopropylmalate dehydrogenase (EC 1.1.1.85... 31 0.93
PPIB_MYCLE (P46697) Probable peptidyl-prolyl cis-trans isomerase... 30 1.6
NLT1_HORVU (P07597) Nonspecific lipid-transfer protein 1 precurs... 30 1.6
>NL22_PARJU (O04403) Probable nonspecific lipid-transfer protein 2
precursor (LTP 2) (Major pollen allergen Par j 2.0102)
(Par j II) (P8 protein)
Length = 133
Score = 43.5 bits (101), Expect = 1e-04
Identities = 36/112 (32%), Positives = 54/112 (48%), Gaps = 12/112 (10%)
Query: 25 VSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGL 84
V AAALA + + E A AP+P + C + ++ CL FV+ K +P K CC L
Sbjct: 11 VIAAALAWTSSAELAS-APAPGEGPCGKVVHHIMPCLKFVKGEEK--EPSKSCCSGTKKL 67
Query: 85 IDGNPIC-----LCKLLGSNTADSFGIKINVNKALKLPTICGVTT--PPVSA 129
+ CK + + T GIK + ++P CG+TT PP++A
Sbjct: 68 SEEVKTTEQKREACKCIVAATKGISGIKNEL--VAEVPKKCGITTTLPPITA 117
>UGP5_ARATH (Q9C7F7) Uncharacterized GPI-anchored protein At1g27950
precursor
Length = 193
Score = 41.2 bits (95), Expect = 7e-04
Identities = 28/109 (25%), Positives = 47/109 (42%), Gaps = 10/109 (9%)
Query: 25 VSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGL 84
+ A+ A +PA LA D C ++ CL F K T P K CC + +
Sbjct: 15 IVASIAAAAPAAPGGALA-----DECNQDFQKVTLCLDFAT--GKATIPSKKCCDAVEDI 67
Query: 85 IDGNPICLCKLLGSNTADSFGIK---INVNKALKLPTICGVTTPPVSAC 130
+ +P CLC ++ +K + +K ++LPT C + ++ C
Sbjct: 68 KERDPKCLCFVIQQAKTGGQALKDLGVQEDKLIQLPTSCQLHNASITNC 116
>NL21_PARJU (P55958) Probable nonspecific lipid-transfer protein 2
precursor (LTP 2) (Major pollen allergen Par j 2.0101)
(Par j II) (P2 protein)
Length = 133
Score = 38.9 bits (89), Expect = 0.003
Identities = 34/112 (30%), Positives = 52/112 (46%), Gaps = 12/112 (10%)
Query: 25 VSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGL 84
V AAALA + + E A AP+P ++ C + ++ CL FV+ K +P K CC L
Sbjct: 11 VIAAALAWTSSAEPAP-APAPGEEACGKVVQDIMPCLHFVKGEEK--EPSKECCSGTKKL 67
Query: 85 IDGNPIC-----LCKLLGSNTADSFGIKINVNKALKLPTICGV--TTPPVSA 129
+ CK + T GIK + ++P C + T PP++A
Sbjct: 68 SEEVKTTEQKREACKCIVRATKGISGIKNEL--VAEVPKKCDIKTTLPPITA 117
>MEN8_SILLA (O24356) MEN-8 protein precursor
Length = 100
Score = 36.2 bits (82), Expect = 0.022
Identities = 17/48 (35%), Positives = 25/48 (51%), Gaps = 3/48 (6%)
Query: 49 GCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLL 96
GC L N++ C +V G+ T P + CC L+G+ N C+C L
Sbjct: 37 GCASQLGNLNVCAPYVVPGAVNTNPSQECCAALSGV---NHDCMCNTL 81
>NLT1_TOBAC (Q42952) Nonspecific lipid-transfer protein 1 precursor
(LTP 1)
Length = 114
Score = 33.9 bits (76), Expect = 0.11
Identities = 28/103 (27%), Positives = 40/103 (38%), Gaps = 19/103 (18%)
Query: 40 VLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLI-------DGNPICL 92
V AP C +N++ CL ++ + T P CC + L+ D C
Sbjct: 17 VAAPCAEAITCGQVTSNLAPCLAYLRN----TGPLGRCCGGVKALVNSARTTEDRQIACT 72
Query: 93 CKLLGSNTADSFGIKINVNKALKLPTICGVTTP----PVSACS 131
C + IN+ KA LP+ CGV P P + CS
Sbjct: 73 CLKSAAGAISG----INLGKAAGLPSTCGVNIPYKISPSTDCS 111
>NLT2_ORYSA (Q42978) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 118
Score = 33.1 bits (74), Expect = 0.19
Identities = 30/108 (27%), Positives = 41/108 (37%), Gaps = 21/108 (19%)
Query: 25 VSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGL 84
V+AA L P A ++ C + +S CL++ GS P CC + L
Sbjct: 12 VAAALLLAGPHTTMAAIS-------CGQVNSAVSPCLSYARGGSG---PSAACCSGVRSL 61
Query: 85 -------IDGNPICLCKLLGSNTADSFGIKINVNKALKLPTICGVTTP 125
D C C N A S +N A +P+ CGVT P
Sbjct: 62 NSAASTTADRRTACNCL---KNVAGSIS-GLNAGNAASIPSKCGVTIP 105
>NLT2_SORBI (Q43194) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 122
Score = 32.7 bits (73), Expect = 0.25
Identities = 31/109 (28%), Positives = 46/109 (41%), Gaps = 17/109 (15%)
Query: 27 AAALAQSPAPETAVLAPSPADDG--CLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGL 84
A A+A A VLA + ++ C + + CL++ + P GCC + L
Sbjct: 8 AVAIAVVAAAAAVVLAATTSEAAVTCGQVSSAIGPCLSYARGQG--SGPSAGCCSGVRSL 65
Query: 85 -------IDGNPICLCKLLGSNTADSFGIK-INVNKALKLPTICGVTTP 125
D C C N A GI+ +NV KA +P+ CGV+ P
Sbjct: 66 NSAARTTADRRAACNCL---KNAAR--GIRGLNVGKAASIPSKCGVSIP 109
>NLT2_LYCES (P27056) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 114
Score = 32.7 bits (73), Expect = 0.25
Identities = 26/103 (25%), Positives = 41/103 (39%), Gaps = 19/103 (18%)
Query: 40 VLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLI-------DGNPICL 92
V+AP C ++ CL +++ L GCC + L+ D C
Sbjct: 17 VVAPHAEALTCGQVTAGLAPCLPYLQGRGPLG----GCCGGVKNLLGSAKTTADRKTACT 72
Query: 93 CKLLGSNTADSFGIKINVNKALKLPTICGVTTP----PVSACS 131
C +N I++NKA +P++C V P P + CS
Sbjct: 73 CLKSAANAIKG----IDLNKAAGIPSVCKVNIPYKISPSTDCS 111
>PRF1_LYCES (Q00451) 36.4 kDa proline-rich protein
Length = 346
Score = 32.3 bits (72), Expect = 0.32
Identities = 24/93 (25%), Positives = 38/93 (40%), Gaps = 9/93 (9%)
Query: 34 PAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLT---KPDKGCCPELAGLID-GNP 89
P P A++ PA C + + C+ + + + CCP L GL+D
Sbjct: 245 PPPPPAIIPSPPAQPTCPIDALKLGACVDVLGGLIHIGIGGSAKQTCCPLLGGLVDLDAA 304
Query: 90 ICLC-----KLLGSNTADSFGIKINVNKALKLP 117
ICLC KLL N +++ ++ K P
Sbjct: 305 ICLCTTIRLKLLNINIILPIALQVLIDDCGKYP 337
>NL41_HORVU (Q43767) Nonspecific lipid-transfer protein 4.1
precursor (LTP 4.1) (CW21) (CW-21)
Length = 115
Score = 32.3 bits (72), Expect = 0.32
Identities = 20/86 (23%), Positives = 33/86 (38%), Gaps = 2/86 (2%)
Query: 40 VLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSN 99
+L + A C + +S C+++ KP CC + L K
Sbjct: 19 LLVAADAAISCGQVSSALSPCISYARGNG--AKPPAACCSGVKRLAGAAQSTADKQAACK 76
Query: 100 TADSFGIKINVNKALKLPTICGVTTP 125
S +N KA +P++CGV+ P
Sbjct: 77 CIKSAAGGLNAGKAAGIPSMCGVSVP 102
>MC1_PINRA (O24493) Male-cone protein 1 precursor (PRMC1)
Length = 92
Score = 32.3 bits (72), Expect = 0.32
Identities = 25/93 (26%), Positives = 37/93 (38%), Gaps = 21/93 (22%)
Query: 40 VLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSN 99
++A A+D C A+ ++ C + V S KP CC L G + C+CK
Sbjct: 19 IVAAVQAED-CSNAMDKLAPCTSAVGLSSNGVKPSSECCDALKGT---STSCVCK----- 69
Query: 100 TADSFGIKINVNKALKLPTICGVTTPPVSACSG 132
+V + LP C + P CSG
Sbjct: 70 ---------SVRAVISLPAKCNL---PALTCSG 90
>CCDP_MAIZE (Q01595) Cortical cell delineating protein precursor
(Root-specific protein ZRP3)
Length = 129
Score = 32.3 bits (72), Expect = 0.32
Identities = 34/135 (25%), Positives = 55/135 (40%), Gaps = 13/135 (9%)
Query: 1 MATTTLVVAIVSLIFASLSFHGDGVSAAALAQSPAPETAVLAPSPADDG---CLMALTNM 57
MA + +SL+FA+ + HG + + P T + P+P+ C + +
Sbjct: 1 MAPKVALFLALSLLFAATA-HGCEPNCSG----PVVPTPPVVPTPSSHSHGRCPIDALKL 55
Query: 58 SDCLTFVEDGSKLTKPDKGCCPELAGLID-GNPICLCKLLGSNTADSFGIKINVNKALK- 115
C + + CCP L GL+D +CLC + +N GI +NV +L
Sbjct: 56 KVCAKVLGLVKVGLPQYEQCCPLLEGLVDLDAALCLCTAIKANV---LGIHLNVPLSLNF 112
Query: 116 LPTICGVTTPPVSAC 130
+ CG P C
Sbjct: 113 ILNNCGRICPEDFTC 127
>NL43_HORVU (Q42842) Nonspecific lipid-transfer protein 4.3
precursor (LTP 4.3)
Length = 115
Score = 32.0 bits (71), Expect = 0.42
Identities = 20/86 (23%), Positives = 33/86 (38%), Gaps = 2/86 (2%)
Query: 40 VLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSN 99
+L + A C + +S C+++ KP CC + L K
Sbjct: 19 LLVATDAAISCGQVSSALSPCISYARGNG--AKPPVACCSGVKRLAGAAQSTADKQAACK 76
Query: 100 TADSFGIKINVNKALKLPTICGVTTP 125
S +N KA +P++CGV+ P
Sbjct: 77 CIKSAAGGLNAGKAAGIPSMCGVSVP 102
>NLT2_ARATH (Q9S7I3) Nonspecific lipid-transfer protein 2 precursor
(LTP 2)
Length = 118
Score = 31.6 bits (70), Expect = 0.55
Identities = 21/77 (27%), Positives = 34/77 (43%), Gaps = 13/77 (16%)
Query: 56 NMSDCLTFVEDGSKLTKPDKGCCPELAGLI-------DGNPICLCKLLGSNTADSFGIKI 108
N++ C+ ++ G+ LT+ GCC + L D C C + A + G +
Sbjct: 35 NLAGCIAYLTRGAPLTQ---GCCNGVTNLKNMASTTPDRQQACRCL---QSAAKAVGPGL 88
Query: 109 NVNKALKLPTICGVTTP 125
N +A LP+ C V P
Sbjct: 89 NTARAAGLPSACKVNIP 105
>NL42_HORVU (Q43875) Nonspecific lipid-transfer protein 4.2
precursor (LTP 4.2) (Low-temperature-responsive protein
4.9)
Length = 115
Score = 31.2 bits (69), Expect = 0.72
Identities = 19/86 (22%), Positives = 33/86 (38%), Gaps = 2/86 (2%)
Query: 40 VLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSN 99
++ + A C + +S C+++ KP CC + L K
Sbjct: 19 LIVATDAAISCGQVSSALSPCISYARGNG--AKPPVACCSGVKRLAGAAQSTADKQAACK 76
Query: 100 TADSFGIKINVNKALKLPTICGVTTP 125
S +N KA +P++CGV+ P
Sbjct: 77 CIKSAAGGLNAGKAAGIPSMCGVSVP 102
>A9_BRANA (Q05772) Tapetum-specific protein A9 precursor
Length = 96
Score = 31.2 bits (69), Expect = 0.72
Identities = 24/75 (32%), Positives = 32/75 (42%), Gaps = 12/75 (16%)
Query: 50 CLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKIN 109
CL L+NM C V G+ P+ CC L N C+C L A +F N
Sbjct: 32 CLDNLSNMQVCAPLVLPGAVNPAPNSNCCIALQAT---NKDCICNAL--RAATTFTTTCN 86
Query: 110 VNKALKLPTI-CGVT 123
LP++ CG+T
Sbjct: 87 ------LPSLDCGIT 95
>LEU3_BACCR (Q81G11) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 354
Score = 30.8 bits (68), Expect = 0.93
Identities = 14/42 (33%), Positives = 21/42 (49%)
Query: 65 EDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGI 106
E G L +P G P++AG NPI + + + SFG+
Sbjct: 262 EKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGL 303
>LEU3_BACAN (Q81T67) 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
(Beta-IPM dehydrogenase) (IMDH) (3-IPM-DH)
Length = 354
Score = 30.8 bits (68), Expect = 0.93
Identities = 14/42 (33%), Positives = 21/42 (49%)
Query: 65 EDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGI 106
E G L +P G P++AG NPI + + + SFG+
Sbjct: 262 EKGPSLYEPIHGSAPDIAGKNKANPIAMMRSVAMMLGQSFGL 303
>PPIB_MYCLE (P46697) Probable peptidyl-prolyl cis-trans isomerase B
(EC 5.2.1.8) (PPIase B) (Rotamase B)
Length = 295
Score = 30.0 bits (66), Expect = 1.6
Identities = 22/109 (20%), Positives = 43/109 (39%), Gaps = 4/109 (3%)
Query: 1 MATTTLVVAIVSLIFASLSFHGDGVSAAALAQSPAPETAVLAPSPADDGCLMALTNMSDC 60
+A ++ + +++ + L D S+ A A +T + PS TN+
Sbjct: 40 VAVAVIITVVATVVISKLGHKHDTASSTASNSLTATKTPAVTPSVLPLPSFQPSTNLGVN 99
Query: 61 LTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTADSFGIKIN 109
+ K KP K P AG + +P + + +N + G+ +N
Sbjct: 100 CQYPPSADKAAKPVK---PPRAGKVPTDPATVSASMATNQG-NIGLLLN 144
>NLT1_HORVU (P07597) Nonspecific lipid-transfer protein 1 precursor
(LTP 1) (Probable amylase/protease inhibitor)
Length = 117
Score = 30.0 bits (66), Expect = 1.6
Identities = 31/108 (28%), Positives = 43/108 (39%), Gaps = 22/108 (20%)
Query: 26 SAAALAQSPAPETAVLAPSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLI 85
+A L + AP AV C + M CLT+V+ G P CC + L
Sbjct: 11 AALVLMLTAAPRAAVAL------NCGQVDSKMKPCLTYVQGGPG---PSGECCNGVRDLH 61
Query: 86 -------DGNPICLCKLLGSNTADSFGI-KINVNKALKLPTICGVTTP 125
D +C C L G + GI +N+N A +P+ C V P
Sbjct: 62 NQAQSSGDRQTVCNC-LKGI----ARGIHNLNLNNAASIPSKCNVNVP 104
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.135 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,338,377
Number of Sequences: 164201
Number of extensions: 653018
Number of successful extensions: 1664
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 29
Number of HSP's that attempted gapping in prelim test: 1657
Number of HSP's gapped (non-prelim): 36
length of query: 139
length of database: 59,974,054
effective HSP length: 99
effective length of query: 40
effective length of database: 43,718,155
effective search space: 1748726200
effective search space used: 1748726200
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146743.12