BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146723.12 + phase: 0
(172 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Y232_HUMAN (Q92628) Hypothetical protein KIAA0232 (Fragment) 35 0.086
GABI_HUMAN (Q13480) GRB2-associated binding protein 1 (GRB2-asso... 33 0.25
HS76_HUMAN (P17066) Heat shock 70 kDa protein 6 (Heat shock 70 k... 30 2.8
ARK1_MOUSE (Q99MK8) Beta-adrenergic receptor kinase 1 (EC 2.7.1.... 30 2.8
ARK1_MESAU (Q64682) Beta-adrenergic receptor kinase 1 (EC 2.7.1.... 30 2.8
HS72_BOVIN (Q27965) Heat shock 70 kDa protein 2 (HSP70-2) 30 3.6
HS71_BOVIN (Q27975) Heat shock 70 kDa protein 1 (HSP70-1) 30 3.6
BIP3_MAIZE (O24581) Luminal binding protein 3 precursor (BiP3) 30 3.6
BIP2_MAIZE (P24067) Luminal binding protein 2 precursor (BiP2) (... 30 3.6
ARK1_RAT (P26817) Beta-adrenergic receptor kinase 1 (EC 2.7.1.12... 30 3.6
YGP7_YEAST (P53111) Hypothetical protein YGL157W 29 4.7
HS76_SAGOE (Q9N1U2) Heat shock 70 kDa protein 6 (Hsp-70-related ... 29 4.7
HS71_PIG (P34930) Heat shock 70 kDa protein 1 (HSP70.1) 29 4.7
HS71_HUMAN (P08107) Heat shock 70 kDa protein 1 (HSP70.1) (HSP70... 29 4.7
HS71_ARATH (P22953) Heat shock cognate 70 kDa protein 1 (Hsc70.1) 29 4.7
G112_HUMAN (Q8IZF6) G-protein coupled receptor GPR112 29 4.7
BIP8_TOBAC (Q03686) Luminal binding protein 8 (BiP 8) (78 kDa gl... 29 4.7
BIP4_TOBAC (Q03684) Luminal binding protein 4 precursor (BiP 4) ... 29 4.7
B3GS_DROME (Q9VLA1) Galactosylgalactosylxylosylprotein 3-beta-gl... 29 4.7
ARK1_HUMAN (P25098) Beta-adrenergic receptor kinase 1 (EC 2.7.1.... 29 4.7
>Y232_HUMAN (Q92628) Hypothetical protein KIAA0232 (Fragment)
Length = 1402
Score = 35.0 bits (79), Expect = 0.086
Identities = 27/87 (31%), Positives = 41/87 (47%), Gaps = 13/87 (14%)
Query: 62 EYK-----FPDPIPEFAD--SETEKFQNHLLNKLSKKDVFEESVEE----VVGVCTEIFS 110
EYK + +PI E+ S K + N+ D+ E+VEE V G+C I +
Sbjct: 402 EYKEEPLWYTEPIAEYFVPLSRKSKLETTYRNRQDTSDLTSEAVEELSESVHGLC--ISN 459
Query: 111 TFLHSEYGGPGTLLVDPFIDMADIVNE 137
LH Y GT + F++M ++NE
Sbjct: 460 NNLHKTYLAAGTFIDGHFVEMPAVINE 486
>GABI_HUMAN (Q13480) GRB2-associated binding protein 1
(GRB2-associated binder-1)
Length = 694
Score = 33.5 bits (75), Expect = 0.25
Identities = 23/90 (25%), Positives = 38/90 (41%), Gaps = 3/90 (3%)
Query: 14 PSSSSSIPSLCFSQTTTKCIQRFSF-PSINRKVSTLPFSSIKRSITRAAEYKFPDPIPEF 72
P + SIP ++ CI PS + +ST+ + +++ + Y P P
Sbjct: 356 PVETCSIPRTASDTDSSYCIPTAGMSPSRSNTISTVDLNKLRKDASSQDCYDIPRAFPSD 415
Query: 73 ADSETEKFQNH--LLNKLSKKDVFEESVEE 100
S E F NH + N L+ V E ++E
Sbjct: 416 RSSSLEGFHNHFKVKNVLTVGSVSSEELDE 445
>HS76_HUMAN (P17066) Heat shock 70 kDa protein 6 (Heat shock 70 kDa
protein B')
Length = 643
Score = 30.0 bits (66), Expect = 2.8
Identities = 14/36 (38%), Positives = 21/36 (57%), Gaps = 4/36 (11%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+L++K+ +E E+ +C IFS YGGPG
Sbjct: 586 NQLAEKEEYEHQKRELEQICRPIFSRL----YGGPG 617
>ARK1_MOUSE (Q99MK8) Beta-adrenergic receptor kinase 1 (EC
2.7.1.126) (Beta-ARK-1) (G-protein coupled receptor
kinase 2)
Length = 689
Score = 30.0 bits (66), Expect = 2.8
Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Query: 72 FADSETEKFQNHLLNKLSKKDVFEESVEEVV-GVCTEIFSTFLHSE 116
F+ + TE Q HL+ K D+F+ +EE+ + ++F F+ S+
Sbjct: 124 FSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESD 169
>ARK1_MESAU (Q64682) Beta-adrenergic receptor kinase 1 (EC
2.7.1.126) (Beta-ARK-1) (G-protein coupled receptor
kinase 2)
Length = 689
Score = 30.0 bits (66), Expect = 2.8
Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Query: 72 FADSETEKFQNHLLNKLSKKDVFEESVEEVV-GVCTEIFSTFLHSE 116
F+ + TE Q HL+ K D+F+ +EE+ + ++F F+ S+
Sbjct: 124 FSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFQKFIESD 169
>HS72_BOVIN (Q27965) Heat shock 70 kDa protein 2 (HSP70-2)
Length = 641
Score = 29.6 bits (65), Expect = 3.6
Identities = 16/36 (44%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N L++KD FE +E+ VC I S L+ GGPG
Sbjct: 584 NTLAEKDEFEHKRKELEQVCNPIISR-LYQGAGGPG 618
>HS71_BOVIN (Q27975) Heat shock 70 kDa protein 1 (HSP70-1)
Length = 641
Score = 29.6 bits (65), Expect = 3.6
Identities = 16/36 (44%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N L++KD FE +E+ VC I S L+ GGPG
Sbjct: 584 NTLAEKDEFEHKRKELEQVCNPIISR-LYQGAGGPG 618
>BIP3_MAIZE (O24581) Luminal binding protein 3 precursor (BiP3)
Length = 663
Score = 29.6 bits (65), Expect = 3.6
Identities = 13/36 (36%), Positives = 20/36 (55%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ ++K+ +EE ++EV VC I S G PG
Sbjct: 614 NQSAEKEDYEEKLKEVEAVCNPIVSAVYQRSGGAPG 649
>BIP2_MAIZE (P24067) Luminal binding protein 2 precursor (BiP2)
(Heat shock protein 70 homolog 2) (B70) (B-70)
Length = 663
Score = 29.6 bits (65), Expect = 3.6
Identities = 13/36 (36%), Positives = 20/36 (55%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ ++K+ +EE ++EV VC I S G PG
Sbjct: 614 NQSAEKEDYEEKLKEVEAVCNPIVSAVYQRSGGAPG 649
>ARK1_RAT (P26817) Beta-adrenergic receptor kinase 1 (EC 2.7.1.126)
(Beta-ARK-1) (G-protein coupled receptor kinase 2)
Length = 689
Score = 29.6 bits (65), Expect = 3.6
Identities = 14/46 (30%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Query: 72 FADSETEKFQNHLLNKLSKKDVFEESVEEVV-GVCTEIFSTFLHSE 116
F+ + TE Q HL+ K D+F+ +EE+ + ++F F+ S+
Sbjct: 124 FSKNATEHVQGHLVKKQVPPDLFQPYIEEICQNLRGDVFHKFIESD 169
>YGP7_YEAST (P53111) Hypothetical protein YGL157W
Length = 347
Score = 29.3 bits (64), Expect = 4.7
Identities = 13/42 (30%), Positives = 23/42 (53%)
Query: 93 VFEESVEEVVGVCTEIFSTFLHSEYGGPGTLLVDPFIDMADI 134
+F +S++ + + I S +HS+ GG PFID+ D+
Sbjct: 208 MFADSLKHGINTSSGIVSELIHSKVGGEFYNYCGPFIDVRDV 249
>HS76_SAGOE (Q9N1U2) Heat shock 70 kDa protein 6 (Hsp-70-related
intracellular vitamin D binding protein)
Length = 643
Score = 29.3 bits (64), Expect = 4.7
Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 4/36 (11%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+L+ K+ +E E+ +C IFS YGGPG
Sbjct: 586 NQLADKEEYEHQKRELEQICRPIFSRL----YGGPG 617
>HS71_PIG (P34930) Heat shock 70 kDa protein 1 (HSP70.1)
Length = 641
Score = 29.3 bits (64), Expect = 4.7
Identities = 16/36 (44%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N L++KD FE +E+ VC I S L+ GGPG
Sbjct: 584 NTLAEKDEFEHKRKELEQVCNPIISG-LYQGAGGPG 618
>HS71_HUMAN (P08107) Heat shock 70 kDa protein 1 (HSP70.1)
(HSP70-1/HSP70-2)
Length = 641
Score = 29.3 bits (64), Expect = 4.7
Identities = 16/36 (44%), Positives = 21/36 (57%), Gaps = 1/36 (2%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N L++KD FE +E+ VC I S L+ GGPG
Sbjct: 584 NTLAEKDEFEHKRKELEQVCNPIISG-LYQGAGGPG 618
>HS71_ARATH (P22953) Heat shock cognate 70 kDa protein 1 (Hsc70.1)
Length = 651
Score = 29.3 bits (64), Expect = 4.7
Identities = 14/44 (31%), Positives = 24/44 (53%), Gaps = 3/44 (6%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLH---SEYGGPGTLLVD 126
N+L++ D FE+ ++E+ +C I + E GGPG +D
Sbjct: 590 NQLAEADEFEDKMKELESICNPIIAKMYQGAGGEAGGPGASGMD 633
>G112_HUMAN (Q8IZF6) G-protein coupled receptor GPR112
Length = 2799
Score = 29.3 bits (64), Expect = 4.7
Identities = 25/80 (31%), Positives = 37/80 (46%), Gaps = 7/80 (8%)
Query: 3 SSTWCLVPFLIPSSSSSIP-----SLCFSQTTTKCIQRFSFPSINRKVSTLPF-SSIKRS 56
+S+W +P S+ IP SL TTT + SFP I+ V T PF +++
Sbjct: 2089 TSSWNRIPTASSPSTLIIPKPTLDSLLNIMTTTSTVPGASFPLISTGV-TYPFTATVSSP 2147
Query: 57 ITRAAEYKFPDPIPEFADSE 76
I+ E + D P F +E
Sbjct: 2148 ISSFFETTWLDSTPSFLSTE 2167
>BIP8_TOBAC (Q03686) Luminal binding protein 8 (BiP 8) (78 kDa
glucose-regulated protein homolog 8) (GRP 78-8)
(Fragment)
Length = 293
Score = 29.3 bits (64), Expect = 4.7
Identities = 12/36 (33%), Positives = 20/36 (55%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ ++K+ +EE ++EV VC I + G PG
Sbjct: 244 NQSAEKEDYEEKLKEVEAVCNPIITAVYQKSGGAPG 279
>BIP4_TOBAC (Q03684) Luminal binding protein 4 precursor (BiP 4) (78
kDa glucose-regulated protein homolog 4) (GRP 78-4)
Length = 667
Score = 29.3 bits (64), Expect = 4.7
Identities = 12/36 (33%), Positives = 20/36 (55%)
Query: 86 NKLSKKDVFEESVEEVVGVCTEIFSTFLHSEYGGPG 121
N+ ++K+ +EE ++EV VC I + G PG
Sbjct: 618 NQSAEKEDYEEKLKEVEAVCNPIITAVYQKSGGAPG 653
>B3GS_DROME (Q9VLA1) Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase S (EC 2.4.1.135)
(Beta-1,3-glucuronyltransferase S)
(Glucuronosyltransferase-S) (GlcAT-S)
(UDP-glucuronosyltransferase-S) (DmGlcAT-BSI)
Length = 409
Score = 29.3 bits (64), Expect = 4.7
Identities = 17/53 (32%), Positives = 25/53 (47%), Gaps = 3/53 (5%)
Query: 105 CTEIFSTFLHSEYGGPGTLLVDPFIDMADIVNERGLPGGPQAARAAINWAQAH 157
C + T L+ +G P T +V P + NE+ P G RAA+ W + H
Sbjct: 173 CNDYMDTLLY-RFGMPFTHMVSPM--PSKFRNEKPAPRGVANRRAALQWIRQH 222
>ARK1_HUMAN (P25098) Beta-adrenergic receptor kinase 1 (EC
2.7.1.126) (Beta-ARK-1) (G-protein coupled receptor
kinase 2)
Length = 689
Score = 29.3 bits (64), Expect = 4.7
Identities = 15/46 (32%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Query: 72 FADSETEKFQNHLLNKLSKKDVFEESVEEVV-GVCTEIFSTFLHSE 116
F+ S TE Q HL K D+F+ +EE+ + ++F F+ S+
Sbjct: 124 FSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESD 169
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.132 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,664,717
Number of Sequences: 164201
Number of extensions: 879353
Number of successful extensions: 2424
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 2413
Number of HSP's gapped (non-prelim): 36
length of query: 172
length of database: 59,974,054
effective HSP length: 102
effective length of query: 70
effective length of database: 43,225,552
effective search space: 3025788640
effective search space used: 3025788640
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 62 (28.5 bits)
Medicago: description of AC146723.12