BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146709.12 + phase: 0 /pseudo
(609 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RHIE_ERWCH (Q8RJP2) Rhamnogalacturonate lyase precursor (EC 4.2.... 92 4e-18
RHGB_ASPAC (Q00019) Rhamnogalacturonase B precursor (EC 4.2.2.-)... 39 0.030
SYV_BACSU (Q05873) Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--t... 33 1.7
VNS2_DSDNV (O71154) Nonstructural protein NS2 33 2.2
ASD1_NEUCR (P78710) Putative rhamnogalacturonase precursor (EC 4... 33 2.8
POLG_FMDVZ (P49303) Genome polyprotein [Contains: Nonstructural ... 32 4.8
41_HUMAN (P11171) Protein 4.1 (Band 4.1) (P4.1) (EPB4.1) (4.1R) 32 4.8
41_BOVIN (Q9N179) Protein 4.1 (Band 4.1) (P4.1) (4.1R) 32 4.8
Y65_BPT7 (P03800) Hypothetical gene 6.5 protein 32 6.3
1A02_HUMAN (P01892) HLA class I histocompatibility antigen, A-2 ... 32 6.3
RASC_DICDI (P32253) Ras-like protein rasC 31 8.3
KAPR_ASPNG (Q9C196) cAMP-dependent protein kinase regulatory sub... 31 8.3
>RHIE_ERWCH (Q8RJP2) Rhamnogalacturonate lyase precursor (EC
4.2.2.-) (Rhamnogalacturonase)
Length = 578
Score = 92.0 bits (227), Expect = 4e-18
Identities = 128/545 (23%), Positives = 212/545 (38%), Gaps = 102/545 (18%)
Query: 82 KNVPINIDIRYIFRSGDSGFYSYAIFNRPKGMPGIEVDQIRFVFKLDKDRFKYMAISDTR 141
+N + ++ I SG YSY + P + V ++R V++ D R + S R
Sbjct: 117 QNGKLRLEYHLIMTRDVSGLYSYVVAANTGSAP-VTVSELRNVYRFDATRLDTLFNSIRR 175
Query: 142 QRNMPAMKDRNTGQVLAYPEAVLLTNPINPQFR---GEVDDKYQYSCENKDTQFMDGLVL 198
G L Y E L + +R G V KY ++ +++++ G++
Sbjct: 176 ------------GTPLLYDELEQLPKVQDETWRLPDGSVYSKYDFAGYQRESRYW-GVMG 222
Query: 199 ILIHQWV---SG**HRVMSFAMVDPLSKILLLMLDQSLFR----THYAGKEVTMAFQEGE 251
W+ SG + D L + LL+ D + +H+ + M Q G
Sbjct: 223 NGYGAWMVPASG------EYYSGDALKQELLVHQDAIILNYLTGSHFGTPD--MVAQPG- 273
Query: 252 TYKKVFGPVFVYLNTASSENDNATLWSDAVQQLSKEVQSWPYDFPQSQDYFPPNQRGAVF 311
++K++GP +Y+N + ++ L +D ++ E SWPY + Y P QR V
Sbjct: 274 -FEKLYGPWLLYIN----QGNDRELVADVSRRAEHERASWPYRWLDDARY--PRQRATVS 326
Query: 312 GRLLVQDWYFEGGRYQYANNAYVGLALPGDAGSWQRESKGYQFWTQTDAKGYFKITNVVP 371
GRL + + + L A ++ ++ GY F +T+ G F ++NV P
Sbjct: 327 GRLRTEA-------------PHATVVLNSSAENFDIQTTGYLFSARTNRDGRFSLSNVPP 373
Query: 372 GHYNLFGWVSGFIGDYKYNSTITITPGGVIKLNSLVYNPPRNGPTIWEIGIPDRLTSEFH 431
G Y L + G G +L + P P W IG DR EF
Sbjct: 374 GEYRLSAYADGGTQIGLLAQQTVRVEGKKTRLGQIDARQP--APLAWAIGQADRRADEFR 431
Query: 432 VPEPYPTLMNKLYTEGRDKQYGLWERYTEMYPTDDLIYTLGVNK-NKDWFYAHVTRNTEN 490
+ + +QY R+ P DL + +G ++ KDW+YA
Sbjct: 432 FGD-------------KPRQY----RWQTEVPA-DLTFEIGKSRERKDWYYAQT------ 467
Query: 491 NTYEPTTWQIIFEHQHDLKSGNYTLQLALASAADAYLQVRFNDRSVYPPHFATGHIGRI- 549
+P +W I+F + YTL +A+A+A++ + + P A G++
Sbjct: 468 ---QPGSWHILFNTR--TPEQPYTLNIAIAAASNNGMTTPASS-----PQLAVKLNGQLL 517
Query: 550 -----RGDNCIQRHGIH-GLYRLFSIDVPSNLLLKGKNIIYLTQTNADTPFQGVMYDYIR 603
D I R + G Y I +P+ L +G N I L VMYD I
Sbjct: 518 TTLKYDNDKSIYRGAMQSGRYHEAHIPLPAGALQQGGNRITLELLGG-----MVMYDAIT 572
Query: 604 LEQPP 608
L + P
Sbjct: 573 LTETP 577
>RHGB_ASPAC (Q00019) Rhamnogalacturonase B precursor (EC 4.2.2.-)
(Rhamnogalacturonan lyase) (RGase B) (RHG B)
Length = 527
Score = 39.3 bits (90), Expect = 0.030
Identities = 75/333 (22%), Positives = 123/333 (36%), Gaps = 43/333 (12%)
Query: 258 GPVFVYLNTASSENDNATLW---SDAVQQLSKEVQ-SWPYDFPQSQDYFPPNQRG-AVFG 312
GP +N+ + + NA W S VQ S + PY S+ P + F
Sbjct: 204 GPFHRDINSNNGGSYNALYWYMNSGHVQTESYRMGLHGPYSMYFSRSGTPSTSIDTSFFA 263
Query: 313 RLLVQDWYFEGGRYQYANNAYVGLALPGDAGSWQRESKGYQFWTQTDAKGYFKITNVVPG 372
L ++ + GR + A A + W ++ Q+WT T + G F + PG
Sbjct: 264 DLDIKGYVAASGRGKVAGTASGADSSMDWVVHWYNDAA--QYWTYTSSSGSFTSPAMKPG 321
Query: 373 HYNLFGWVSGFIGDYKY-NSTITITPGGVIKLNSLVYNPPRNGPTIWEIGIPDRLTSEFH 431
Y + + G+Y S++T++ G N + + G TI++IG D
Sbjct: 322 TYTMV----YYQGEYAVATSSVTVSAGSTTTKN--ISGSVKTGTTIFKIGEWD------G 369
Query: 432 VPEPYPTLMNKLYTEGRDKQYGLWERYTEMYPTDDLIYTLGVNKNKDWFYAHVTRNTENN 491
P + N+L D + W L YT+G + D F V ++ N
Sbjct: 370 QPTGFRNAANQLRMHPSDSRMSSW---------GPLTYTVGSSALTD-FPMAVFKSVNN- 418
Query: 492 TYEPTTWQIIFEHQHDLKSGNYTLQLALA-SAADAYLQVRFNDRSVYPPHFATGHIGRIR 550
P T + ++G TL++ S A Q N + P T R
Sbjct: 419 ---PVTIKFT---ATSAQTGAATLRIGTTLSFAGGRPQATINSYTGSAPAAPTNLDSR-- 470
Query: 551 GDNCIQRHGIHGLYRLFSIDVPSNLLLKGKNII 583
+ R GL ++ + +PS ++ G N I
Sbjct: 471 ---GVTRGAYRGLGEVYDVSIPSGTIVAGTNTI 500
>SYV_BACSU (Q05873) Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA
ligase) (ValRS)
Length = 880
Score = 33.5 bits (75), Expect = 1.7
Identities = 32/131 (24%), Positives = 52/131 (39%), Gaps = 29/131 (22%)
Query: 278 SDAVQQLSKE---VQSWPYDFPQSQDYFPPNQRGAVFGRLL-----VQDWYFEGGRYQYA 329
+DA +L K+ V P F ++ ++ N R R L + WY ++
Sbjct: 372 ADAAIELEKKEEKVNFVPDRFEKTYLHWMENIRDWCISRQLWWGHRIPAWY-----HKET 426
Query: 330 NNAYVGLALPGDAGSWQRESKGYQFW----------------TQTDAKGYFKITNVVPGH 373
YVGL P D+ +W++++ W T D K Y+ +V G+
Sbjct: 427 GELYVGLEAPEDSENWEQDTDVLDTWFSSALWPFSTMGWPDVTAEDFKRYYPTDVLVTGY 486
Query: 374 YNLFGWVSGFI 384
+F WVS I
Sbjct: 487 DIIFFWVSRMI 497
>VNS2_DSDNV (O71154) Nonstructural protein NS2
Length = 275
Score = 33.1 bits (74), Expect = 2.2
Identities = 25/99 (25%), Positives = 42/99 (42%), Gaps = 2/99 (2%)
Query: 390 NSTITITPGGVIKLNSLVYNPPRNGPTIWEIGIPDRLTSEFHVPEPYPTLMNKLYTEGRD 449
+STI +TP GV + LV + P ++ P ++ + P + LY G
Sbjct: 135 SSTIALTPIGVAGTSGLVKSSP--SELFRKLANPSNISGSLNEPTGMMSSSISLYENGES 192
Query: 450 KQYGLWERYTEMYPTDDLIYTLGVNKNKDWFYAHVTRNT 488
QY L E+ + T ++ K + W+ A + R T
Sbjct: 193 VQYTLEEKVGKYRVTMNVYDGPESLKKEKWYQAPIARIT 231
>ASD1_NEUCR (P78710) Putative rhamnogalacturonase precursor (EC
4.2.2.-) (Rhamnogalacturonan lyase) (RGase) (Ascus
development protein 1) (Asd-I)
Length = 540
Score = 32.7 bits (73), Expect = 2.8
Identities = 69/287 (24%), Positives = 105/287 (36%), Gaps = 53/287 (18%)
Query: 301 YFPPNQRGAVFGRLLVQDWYFEGGRYQYANNAYVGLALPGDAGSWQRESKGYQFWTQTDA 360
Y P +QRG V GR FEG + Y + Q+W +T
Sbjct: 282 YVPDSQRGTVIGRATGIPSGFEGVVHWY--------------------NAAAQYWVRTAP 321
Query: 361 KGYFKITNVVPGHYNLFGWVSGFIGDYKYNSTITITPGGVIKLNSLVYNPPRNGPTIWEI 420
G F + PG Y + + + F ST+T++ G S+ + T+++I
Sbjct: 322 NGDFTSPKMKPGTYTMVLYQTEF---KVATSTVTVSAGKTTTA-SIASTFNTSHTTLFKI 377
Query: 421 GIPDRLTSEFHVPEPYPTLMNKLYTEGRDKQYGLWERYTEMYPTDDLIYTLGVNKNKDWF 480
G D + F + + L D + W L YT+G + D F
Sbjct: 378 GEYDGQPTGFRNADKF------LRMHPSDSRMSSW---------GPLTYTVGSSSLND-F 421
Query: 481 YAHVTRNTENNTYEPTTWQIIFEHQHDLKSGNYTLQLALA-SAADAYLQVRFNDRSVYPP 539
V ++ N P T I + S TL++A S A A QV N S P
Sbjct: 422 PMAVFKSVNN----PVT---IKFNLGSAPSQATTLRIATTLSFAGARPQVVVNGWSA-PA 473
Query: 540 HFATGHIGRIRGDNCIQRHGIHGLYRLFSIDVPSNLLLKGKNIIYLT 586
A I RG + R G ++ + VP+ L+ G N I ++
Sbjct: 474 PAAPAKIDS-RG---VTRGAYRGYGEVYDVAVPAGKLISGTNTITIS 516
>POLG_FMDVZ (P49303) Genome polyprotein [Contains: Nonstructural
protein P20A; Coat protein VP4; Coat protein VP3; Coat
protein VP2; Coat protein VP1; Core protein X; Core
protein p14; Core protein p41; Core protein p19;
Genome-linked protein VPG1; Genome
Length = 2336
Score = 32.0 bits (71), Expect = 4.8
Identities = 25/92 (27%), Positives = 36/92 (38%), Gaps = 14/92 (15%)
Query: 356 TQTDAKGYFKI-TNVVPGHYNLFGWVSGFIGDYK------YNSTITITPGGVIKLNSLVY 408
T T + G K+ N+ P H ++ G + G Y +T P + +VY
Sbjct: 475 TNTVSAGQIKVYANIAPTHVHVAGELPSKEGIVPVACSDGYGGLVTTDPKTADPVYGMVY 534
Query: 409 NPPRNGPTIWEIGIPDRLTSEFHVPEPYPTLM 440
NPPR P R T+ V E PT +
Sbjct: 535 NPPRT-------NYPGRFTNLLDVAEACPTFL 559
>41_HUMAN (P11171) Protein 4.1 (Band 4.1) (P4.1) (EPB4.1) (4.1R)
Length = 864
Score = 32.0 bits (71), Expect = 4.8
Identities = 11/43 (25%), Positives = 25/43 (57%)
Query: 263 YLNTASSENDNATLWSDAVQQLSKEVQSWPYDFPQSQDYFPPN 305
Y A +N + W D+ +++ K+V+ P++F + ++PP+
Sbjct: 250 YFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPD 292
>41_BOVIN (Q9N179) Protein 4.1 (Band 4.1) (P4.1) (4.1R)
Length = 617
Score = 32.0 bits (71), Expect = 4.8
Identities = 11/43 (25%), Positives = 25/43 (57%)
Query: 263 YLNTASSENDNATLWSDAVQQLSKEVQSWPYDFPQSQDYFPPN 305
Y A +N + W D+ +++ K+V+ P++F + ++PP+
Sbjct: 41 YFGLAIWDNATSKTWLDSAKEIKKQVRGVPWNFTFNVKFYPPD 83
>Y65_BPT7 (P03800) Hypothetical gene 6.5 protein
Length = 84
Score = 31.6 bits (70), Expect = 6.3
Identities = 17/37 (45%), Positives = 21/37 (55%), Gaps = 4/37 (10%)
Query: 522 AADAYLQVRFNDRSVYPPHFATGHIGRIRGDNCIQRH 558
A YLQVRFN Y A+GHIG +R + C + H
Sbjct: 21 ATAEYLQVRFN----YAYLEASGHIGLMRANGCSEAH 53
>1A02_HUMAN (P01892) HLA class I histocompatibility antigen, A-2
alpha chain precursor (MHC class I antigen A*2)
Length = 365
Score = 31.6 bits (70), Expect = 6.3
Identities = 19/81 (23%), Positives = 35/81 (42%), Gaps = 5/81 (6%)
Query: 270 ENDNATLWSDAVQQLSKEVQSWPYDFPQSQDYFPPNQRGA-----VFGRLLVQDWYFEGG 324
E + W +++ Q+ D + Y+ ++ G+ ++G + DW F G
Sbjct: 77 EQEGPEYWDGETRKVKAHSQTHRVDLGTLRGYYNQSEAGSHTVQRMYGCDVGSDWRFLRG 136
Query: 325 RYQYANNAYVGLALPGDAGSW 345
+QYA + +AL D SW
Sbjct: 137 YHQYAYDGKDYIALKEDLRSW 157
>RASC_DICDI (P32253) Ras-like protein rasC
Length = 189
Score = 31.2 bits (69), Expect = 8.3
Identities = 19/62 (30%), Positives = 32/62 (50%), Gaps = 6/62 (9%)
Query: 249 EGETYKKVFGPVFVYLNTASSENDNATLWSDAVQQLSKEVQSWPYDFPQSQDYFPPNQRG 308
EG+ K FG F L T++ N +A L +E++ W + PQ+++ PP +RG
Sbjct: 132 EGKELAKSFGAPF--LETSAKSRVNV---EEAFFTLVREIKRWNQN-PQNEEMLPPKKRG 185
Query: 309 AV 310
+
Sbjct: 186 CI 187
>KAPR_ASPNG (Q9C196) cAMP-dependent protein kinase regulatory
subunit (PKA regulatory subunit)
Length = 411
Score = 31.2 bits (69), Expect = 8.3
Identities = 28/90 (31%), Positives = 41/90 (45%), Gaps = 16/90 (17%)
Query: 349 SKGYQFWTQTDAKGYFKITNVVPGHYNLFGWVSGFIG---DYKYNSTITITPGGVIKLNS 405
+KG + +Q DA YF I V GH++ SG + D N ++ PGG +
Sbjct: 183 AKGIKVISQGDAGDYFYI--VENGHFDFMIHPSGSVQPGPDGMGNKVGSVGPGGSFGELA 240
Query: 406 LVYNPPRNG--------PTIWEIGIPDRLT 427
L+YN PR T+W + DR+T
Sbjct: 241 LMYNAPRAATVVSVDPKSTLWAL---DRIT 267
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.140 0.436
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 79,748,633
Number of Sequences: 164201
Number of extensions: 3666040
Number of successful extensions: 6431
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 6420
Number of HSP's gapped (non-prelim): 14
length of query: 609
length of database: 59,974,054
effective HSP length: 116
effective length of query: 493
effective length of database: 40,926,738
effective search space: 20176881834
effective search space used: 20176881834
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 69 (31.2 bits)
Medicago: description of AC146709.12