BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146706.7 + phase: 0 /pseudo
         (617 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

LCFA_SALTY (P63521) Long-chain-fatty-acid--CoA ligase (EC 6.2.1....   134  5e-31
LCFA_SALTI (P63522) Long-chain-fatty-acid--CoA ligase (EC 6.2.1....   134  5e-31
LCFA_ECOLI (P29212) Long-chain-fatty-acid--CoA ligase (EC 6.2.1....   134  7e-31
LCFA_ECO57 (Q8XDR6) Long-chain-fatty-acid--CoA ligase (EC 6.2.1....   133  1e-30
LCF1_CAVPO (Q9JID6) Long-chain-fatty-acid--CoA ligase 1 (EC 6.2....   133  1e-30
LCFH_HAEIN (P44446) Putative long-chain-fatty-acid--CoA ligase (...   127  7e-29
LCF6_RAT (P33124) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.1....   105  5e-22
4CL2_TOBAC (O24146) 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL...   104  8e-22
4CL_PINTA (P41636) 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL) (...   101  7e-21
LUCI_LUCLA (Q01158) Luciferin 4-monooxygenase (EC 1.13.12.7) (Lu...    97  2e-19
LUCI_PHOPY (P08659) Luciferin 4-monooxygenase (EC 1.13.12.7) (Lu...    96  2e-19
LUCI_LUCCR (P13129) Luciferin 4-monooxygenase (EC 1.13.12.7) (Lu...    95  5e-19
4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL...    88  8e-17
LCFA_BACSU (P94547) Long-chain-fatty-acid--CoA ligase (EC 6.2.1....    87  1e-16
4CL_VANPL (O24540) 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL) (...    87  1e-16
4CL1_SOYBN (P31686) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL...    85  6e-16
4CL1_ORYSA (P17814) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL...    85  6e-16
4CL1_TOBAC (O24145) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL...    84  1e-15
4CL2_ARATH (Q9S725) 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL...    84  1e-15
4CL2_SOLTU (P31685) 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL...    82  5e-15

>LCFA_SALTY (P63521) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
           (Long-chain acyl-CoA synthetase)
          Length = 561

 Score =  134 bits (338), Expect = 5e-31
 Identities = 139/508 (27%), Positives = 231/508 (45%), Gaps = 78/508 (15%)

Query: 97  DEWKTVPDIWRSSAEKYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRV-IGVSPNE 155
           D ++++ +++  +A +Y D+ A ++        MT+++LE+    FA  L+  +G+   +
Sbjct: 20  DRYQSLVELFEHAATRYADQPAFVNM----GEVMTFRKLEERSRAFAAYLQQGLGLKKGD 75

Query: 156 KIALFADNSCRWLVADQGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNR 215
           ++AL   N  ++ VA  G++  G I V      +  EL    N S + A+ + +    + 
Sbjct: 76  RVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAAAIIIVS-NFAHT 134

Query: 216 IAKAFDLKASMRFVIL------LWGEKSCLVNEGSKEVPIFTFTEIMHLGRG-SRRLFES 268
           + K  + K S++ VIL      L   K  +VN   K +         HL    S R    
Sbjct: 135 LEKVVE-KTSVQHVILTRMGDQLSTAKGTVVNFVVKYIK--RLVPKYHLPDAISFRSALQ 191

Query: 269 HDARKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAASLACI*KSL*V 328
           H  R  YV   + ++D+A L YT GTTG  KG MLTH+N+L  + +  A+          
Sbjct: 192 HGYRMQYVKPEVVAEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVKATYG-------- 243

Query: 329 FHFFMRC*PSIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTFIRVSLGYMECKRIYE 388
                   P ++  ++L V             T+  +  + ALT        M C    E
Sbjct: 244 --------PLLHPGKELVV-------------TALPLYHIFALT--------MNCLLFIE 274

Query: 389 ----GKCLTKNQKSPSYLYAMLDWLGARIIATILFPIHMLAKKLVYSKIHSAIGFSKAGI 444
                  +T  +  P  +  +     A+   T +  ++ L   L+ +K    + FS   +
Sbjct: 275 LGGQNLLITNPRDIPGLVKEL-----AKYPFTAMTGVNTLFNALLNNKEFQQLDFSSLHL 329

Query: 445 SGGGSLPSH---VDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNV-IGSVGHPLKHTEF 500
           S GG +P      +R+ +  G  L  GYGLTE +P+++      +   GS+G P+  TE 
Sbjct: 330 SAGGGMPVQNVVAERWVKLTGQYLLEGYGLTECAPLVSVNPHDIDYHSGSIGLPVPSTEA 389

Query: 501 KVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWIAAYHS 560
           K+VD +  EV P G  G L V+GPQ+M GY++ P AT++ I KDGWL+TGDI  +     
Sbjct: 390 KLVDDDDNEVAP-GEAGELCVKGPQVMLGYWQRPDATDEII-KDGWLHTGDIAVM----- 442

Query: 561 SGRSRNCGGVIVVEGRAKDTIVLSSEKV 588
                +  G + +  R KD I++S   V
Sbjct: 443 -----DEDGFLRIVDRKKDMILVSGFNV 465


>LCFA_SALTI (P63522) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
           (Long-chain acyl-CoA synthetase)
          Length = 561

 Score =  134 bits (338), Expect = 5e-31
 Identities = 139/508 (27%), Positives = 231/508 (45%), Gaps = 78/508 (15%)

Query: 97  DEWKTVPDIWRSSAEKYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRV-IGVSPNE 155
           D ++++ +++  +A +Y D+ A ++        MT+++LE+    FA  L+  +G+   +
Sbjct: 20  DRYQSLVELFEHAATRYADQPAFVNM----GEVMTFRKLEERSRAFAAYLQQGLGLKKGD 75

Query: 156 KIALFADNSCRWLVADQGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNR 215
           ++AL   N  ++ VA  G++  G I V      +  EL    N S + A+ + +    + 
Sbjct: 76  RVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAAAIIIVS-NFAHT 134

Query: 216 IAKAFDLKASMRFVIL------LWGEKSCLVNEGSKEVPIFTFTEIMHLGRG-SRRLFES 268
           + K  + K S++ VIL      L   K  +VN   K +         HL    S R    
Sbjct: 135 LEKVVE-KTSVQHVILTRMGDQLSTAKGTVVNFVVKYIK--RLVPKYHLPDAISFRSALQ 191

Query: 269 HDARKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAASLACI*KSL*V 328
           H  R  YV   + ++D+A L YT GTTG  KG MLTH+N+L  + +  A+          
Sbjct: 192 HGYRMQYVKPEVVAEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVKATYG-------- 243

Query: 329 FHFFMRC*PSIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTFIRVSLGYMECKRIYE 388
                   P ++  ++L V             T+  +  + ALT        M C    E
Sbjct: 244 --------PLLHPGKELVV-------------TALPLYHIFALT--------MNCLLFIE 274

Query: 389 ----GKCLTKNQKSPSYLYAMLDWLGARIIATILFPIHMLAKKLVYSKIHSAIGFSKAGI 444
                  +T  +  P  +  +     A+   T +  ++ L   L+ +K    + FS   +
Sbjct: 275 LGGQNLLITNPRDIPGLVKEL-----AKYPFTAMTGVNTLFNALLNNKEFQQLDFSSLHL 329

Query: 445 SGGGSLPSH---VDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNV-IGSVGHPLKHTEF 500
           S GG +P      +R+ +  G  L  GYGLTE +P+++      +   GS+G P+  TE 
Sbjct: 330 SAGGGMPVQNVVAERWVKLTGQYLLEGYGLTECAPLVSVNPHDIDYHSGSIGLPVPSTEA 389

Query: 501 KVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWIAAYHS 560
           K+VD +  EV P G  G L V+GPQ+M GY++ P AT++ I KDGWL+TGDI  +     
Sbjct: 390 KLVDDDDNEVAP-GEAGELCVKGPQVMLGYWQRPDATDEII-KDGWLHTGDIAVM----- 442

Query: 561 SGRSRNCGGVIVVEGRAKDTIVLSSEKV 588
                +  G + +  R KD I++S   V
Sbjct: 443 -----DEDGFLRIVDRKKDMILVSGFNV 465


>LCFA_ECOLI (P29212) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
           (Long-chain acyl-CoA synthetase)
          Length = 561

 Score =  134 bits (337), Expect = 7e-31
 Identities = 134/506 (26%), Positives = 228/506 (44%), Gaps = 74/506 (14%)

Query: 97  DEWKTVPDIWRSSAEKYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRV-IGVSPNE 155
           D ++++ D++  S  +Y D+ A ++        MT+++LE+    FA  L+  +G+   +
Sbjct: 20  DRYQSLVDMFEQSVARYADQPAFVNM----GEVMTFRKLEERSRAFAAYLQQGLGLKKGD 75

Query: 156 KIALFADNSCRWLVADQGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNR 215
           ++AL   N  ++ VA  G++  G I V      +  EL    N S + A+ + +    + 
Sbjct: 76  RVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVIVS-NFAHT 134

Query: 216 IAKAFDLKASMRFVILLWGE-----KSCLVNEGSKEVPIFTFTEIMHLGRGSRRLFESHD 270
           + K  D  A    ++   G+     K  +VN   K +         HL          H+
Sbjct: 135 LEKVVDKTAVQHVILTRMGDQLSTAKGTVVNFVVKYIK--RLVPKYHLPDAISFRSALHN 192

Query: 271 A-RKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAASLACI*KSL*VF 329
             R  YV   +  +D+A L YT GTTG  KG MLTH+N+L  + +  A+           
Sbjct: 193 GYRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVNATYG--------- 243

Query: 330 HFFMRC*PSIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTF---IRVSLGYMECKRI 386
                  P ++  ++L V             T+  +  + ALT    + + LG       
Sbjct: 244 -------PLLHPGKELVV-------------TALPLYHIFALTINCLLFIELGG------ 277

Query: 387 YEGKCLTKNQKSPSYLYAMLDWLGARIIATILFPIHMLAKKLVYSKIHSAIGFSKAGISG 446
            +   +T  +  P  +  +     A+   T +  ++ L   L+ +K    + FS   +S 
Sbjct: 278 -QNLLITNPRDIPGLVKEL-----AKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSA 331

Query: 447 GGSLPSH---VDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNV-IGSVGHPLKHTEFKV 502
           GG +P      +R+ +  G  L  GYGLTE +P+++      +   GS+G P+  TE K+
Sbjct: 332 GGGMPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKL 391

Query: 503 VDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWIAAYHSSG 562
           VD +  EV PPG  G L V+GPQ+M GY++ P AT++ I K+GWL+TGDI  +       
Sbjct: 392 VDDDDNEV-PPGQPGELCVKGPQVMLGYWQRPDATDEII-KNGWLHTGDIAVM------- 442

Query: 563 RSRNCGGVIVVEGRAKDTIVLSSEKV 588
              +  G + +  R KD I++S   V
Sbjct: 443 ---DEEGFLRIVDRKKDMILVSGFNV 465


>LCFA_ECO57 (Q8XDR6) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
           (Long-chain acyl-CoA synthetase)
          Length = 561

 Score =  133 bits (335), Expect = 1e-30
 Identities = 135/506 (26%), Positives = 227/506 (44%), Gaps = 74/506 (14%)

Query: 97  DEWKTVPDIWRSSAEKYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRV-IGVSPNE 155
           D ++++ D++  S  +Y D+ A ++        MT+++LE+    FA  L+  +G+   +
Sbjct: 20  DRYQSLVDMFEQSVARYADQPAFVNM----GEVMTFRKLEERSRAFAAYLQQGLGLKKGD 75

Query: 156 KIALFADNSCRWLVADQGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNR 215
           ++AL   N  ++ VA  G++  G I V      +  EL    N S + A+ + +    + 
Sbjct: 76  RVALMMPNLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVIVS-NFAHT 134

Query: 216 IAKAFDLKASMRFVILLWGE-----KSCLVNEGSKEVPIFTFTEIMHLGRGSRRLFESHD 270
           + K  D  A    ++   G+     K  LVN   K +         HL          H+
Sbjct: 135 LEKVVDKTAVQHVILTRMGDQLSTAKGTLVNFVVKYIK--RLVPKYHLPDAISFRSALHN 192

Query: 271 A-RKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAASLACI*KSL*VF 329
             R  YV   +  +D+A L YT GTTG  KG MLTH+N+L  + +  A+           
Sbjct: 193 GYRMQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVNATYG--------- 243

Query: 330 HFFMRC*PSIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTF---IRVSLGYMECKRI 386
                  P ++  ++L V             T+  +  + ALT    + + LG       
Sbjct: 244 -------PLLHPGKELVV-------------TALPLYHIFALTINCLLFIELGG------ 277

Query: 387 YEGKCLTKNQKSPSYLYAMLDWLGARIIATILFPIHMLAKKLVYSKIHSAIGFSKAGISG 446
            +   +T  +  P  +  +     A+   T +  ++ L   L+ +K    + FS   +S 
Sbjct: 278 -QNLLITNPRDIPGLVKEL-----AKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSA 331

Query: 447 GGSLPSH---VDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNV-IGSVGHPLKHTEFKV 502
           GG +P      +R+ +  G  L  GYGLTE +P+++      +   GS+G P+  TE K+
Sbjct: 332 GGGMPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKL 391

Query: 503 VDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWIAAYHSSG 562
           VD +  EV  PG  G L VRGPQ+M GY++ P AT++ I K+GWL+TGDI  +       
Sbjct: 392 VDDDDNEV-SPGQPGELCVRGPQVMLGYWQRPDATDEII-KNGWLHTGDIAVM------- 442

Query: 563 RSRNCGGVIVVEGRAKDTIVLSSEKV 588
              +  G + +  R KD I++S   V
Sbjct: 443 ---DEEGFLRIVDRKKDMILVSGFNV 465


>LCF1_CAVPO (Q9JID6) Long-chain-fatty-acid--CoA ligase 1 (EC
           6.2.1.3) (Long-chain acyl-CoA synthetase 1) (LACS 1)
           (Palmitoyl-CoA ligase)
          Length = 698

 Score =  133 bits (335), Expect = 1e-30
 Identities = 131/490 (26%), Positives = 225/490 (45%), Gaps = 36/490 (7%)

Query: 85  SSLLSGNDGVVS--DEWKTVPDIWRSSAEKYGDKIALIDPYHDPP-STMTYKQLEDAILD 141
           S+LL  ++ +V   D+ +T+ D+++   +   +   L     D P   ++YKQ+ED    
Sbjct: 73  STLLDSDEPLVYFYDDVRTLYDVFQRGIQVSNNGPCLGSRKPDQPYEWLSYKQVEDLSEC 132

Query: 142 FAEGLRVIG--VSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSSIEELLQIYNH 199
               L   G   SP++ I +FA N   W++ +Q   A   + V        + +  I N 
Sbjct: 133 IGSALLQKGFQASPDQFIGIFAQNRPEWVIIEQACFAYSMVVVPLYDTLGADAITYIVNK 192

Query: 200 SESIALAVDNPEMFNRIAKAFDLKAS--MRFVILLWGEKSCLVNEGSK-EVPIFTFTEIM 256
           +E   +  D PE    + ++ + K +  ++ ++++    S LV +G K  V + +   + 
Sbjct: 193 AELSVIFADKPEKARILLESVENKLTPGLKIIVVMDSYGSELVEQGKKCGVEVISLKAME 252

Query: 257 HLGRGSRRLFESHDARKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKY- 315
            LGR +RR  +  +            DD+A + +TSGTTGNPKG M+TH+N++   S + 
Sbjct: 253 GLGRANRRKPKPPEP-----------DDLAVICFTSGTTGNPKGAMITHKNVVSDCSAFV 301

Query: 316 -AASLACI*KSL*VFHFFMRC*PSIYNCEKLEVYESLYSGIQ---RQISTSSLVRKLVAL 371
            A   A +  +  +   F+   P  +  E+L     L  G +    Q     L+  L AL
Sbjct: 302 KATEKALVLNASDIHISFL---PLAHMYEQLLQCVMLCHGAKIGFFQGDIRLLMDDLKAL 358

Query: 372 TFIRVSLGYMECKRIYEGKCLTKNQKSPSYLYAMLDWLGARIIATILFPI---HMLAKKL 428
                 +      R+++      N     +L   LD+   R  A +   I   + +  KL
Sbjct: 359 QPTIFPVVPRLLNRMFDRIFAQANTTVKRWL---LDFASKRKEAELRSGIIRNNSVWDKL 415

Query: 429 VYSKIHSAIGFS-KAGISGGGSLPSHVDRFFE-AIGVTLQNGYGLTETSPVIAARRLSCN 486
           ++ KI S++G   +  ++G   + + V  F   A+G     GYG TE +   +       
Sbjct: 416 IFHKIQSSLGGKVRLMVTGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCSLSVPGDW 475

Query: 487 VIGSVGHPLKHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGW 546
             G VG P+     K+VD E    +    +G + V+GP + KGY K+P+ T +A+DKDGW
Sbjct: 476 TAGHVGAPMPCNFIKLVDVEEMNYMAAMGEGEVCVKGPNVFKGYLKDPAKTAEALDKDGW 535

Query: 547 LNTGDIG-WI 555
           L+TGDIG W+
Sbjct: 536 LHTGDIGKWL 545


>LCFH_HAEIN (P44446) Putative long-chain-fatty-acid--CoA ligase (EC
           6.2.1.3) (Long-chain acyl-CoA synthetase) (LACS)
          Length = 607

 Score =  127 bits (320), Expect = 7e-29
 Identities = 120/441 (27%), Positives = 197/441 (44%), Gaps = 47/441 (10%)

Query: 130 MTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSS 189
           +++K  ++ +   +  L    +   +KIA+FA N  RW + D   +   AI V   + ++
Sbjct: 43  ISWKNFQEQLNQLSRALLAHNIDVQDKIAIFAHNMERWTIVDIATLQIRAITVPIYATNT 102

Query: 190 IEELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVILLWGEKSCLVNEGSKEVPI 249
            ++   I NH++   L V + E +++  +       ++ ++ +   KS +  +  ++   
Sbjct: 103 AQQAEFILNHADVKILFVGDQEQYDQTLEIAHHCPKLQKIVAM---KSTI--QLQQDPLS 157

Query: 250 FTFTEIMHLGRGSRRLFESHDARKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLL 309
            T+   +  G  +++   +    +  +       D+ T++YTSGTTG PKGVML + NL 
Sbjct: 158 CTWESFIKTGSNAQQDELTQRLNQKQL------SDLFTIIYTSGTTGEPKGVMLDYANLA 211

Query: 310 HQISKYAASLACI*KSL*VFHFFMRC*PSIYNCEKLEVYESLYSG-IQRQISTSSLVRKL 368
           HQ+  +  SL    + + +        P  +  E+      L+ G I   +  ++ VR  
Sbjct: 212 HQLETHDLSLNVTDQDISLSFL-----PFSHIFERAWAAYILHRGAILCYLEDTNQVRS- 265

Query: 369 VALTFIRVSLGYMECKRIYE---GKCLTKNQKSPSYLYAMLDW------------LGARI 413
            ALT IR +L      R YE      L K QK+P     M  W               + 
Sbjct: 266 -ALTEIRPTL-MCAVPRFYEKIYAAVLDKVQKAPKLRQIMFHWAISVGQKYFDLRANNKA 323

Query: 414 IATILFPIHMLAKKLVYSKIHSAIGFS-KAGISGGGSLPSHVDRFFEAIGVTLQNGYGLT 472
           I  +L     LA KLV SK+   +G   K    GG  L   +  FF AIG+ ++ GYG+T
Sbjct: 324 IPFLLKKQFALADKLVLSKLRQLLGGRIKMMPCGGAKLEPAIGLFFHAIGINIKLGYGMT 383

Query: 473 ETSPVIAARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYK 532
           ET+  ++          S+G  +   E K+   E  E+L         VRG  +MKGYYK
Sbjct: 384 ETTATVSCWHDFQFNPNSIGTLMPKAEVKI--GENNEIL---------VRGGMVMKGYYK 432

Query: 533 NPSATNQAIDKDGWLNTGDIG 553
            P  T QA  +DG+L TGD G
Sbjct: 433 KPEETAQAFTEDGFLKTGDAG 453


>LCF6_RAT (P33124) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.1.3)
           (Long-chain acyl-CoA synthetase 6) (LACS 6)
           (Long-chain-fatty-acid--CoA ligase, brain isozyme)
          Length = 697

 Score =  105 bits (261), Expect = 5e-22
 Identities = 107/422 (25%), Positives = 174/422 (40%), Gaps = 37/422 (8%)

Query: 151 VSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNP 210
           V   + I +FA N   W++A+        + V          +  I N ++   + VD P
Sbjct: 144 VGTEQFIGVFAQNRPEWIIAELACYTYSMVVVPLYDTLGPGSIRYIINTADICTVIVDKP 203

Query: 211 EMFNRIAKAFDLKAS--MRFVILLWGEKSCLVNEGSKEVPIFTFTEIMHLGRGSRRLFES 268
                + +  + K +  ++ VIL+      L   G K            +   S +  E 
Sbjct: 204 HKAILLLEHVERKETPGLKLVILMEPFDDALRERGKK----------CGVDIKSMQAIED 253

Query: 269 HDARKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQIS--------KYAASLA 320
                H V    + DD++ + +TSGTTGNPKG MLTH N++   S        ++A + A
Sbjct: 254 SGQENHRVPVPPRPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTESQWAPTCA 313

Query: 321 CI*KS-L*VFHFFMRC*PSIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTFIRVSLG 379
            +  S L + H F R   S+  C    V    + G  R +S      +      +   L 
Sbjct: 314 DVHFSYLPLAHMFERMVQSVVYCHGGRV--GFFQGDIRLLSDDMKALRPTIFPVVPRLLN 371

Query: 380 YMECKRIYEGKCLTKNQKSPSYLYAMLDWLGARIIATILFPI---HMLAKKLVYSKIHSA 436
            M  K  ++     K          +L++   R  A +   I   + +  +L ++KI ++
Sbjct: 372 RMYDKIFHQADTSLKRW--------LLEFAAKRKQAEVRSGIIRNNSIWDELFFNKIQAS 423

Query: 437 IGFSKAGISGGGS--LPSHVDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNVIGSVGHP 494
           +G     I  G +   P+ +     A+G  +  GYG TE +             G VG P
Sbjct: 424 LGGHVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAP 483

Query: 495 LKHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIG- 553
           L     K+VD+E         +G + V+GP + KGY K+   T +A+D DGWL+TGDIG 
Sbjct: 484 LPCNHIKLVDAEELNYWTSKGEGEICVKGPNVFKGYLKDEDRTKEALDSDGWLHTGDIGK 543

Query: 554 WI 555
           W+
Sbjct: 544 WL 545


>4CL2_TOBAC (O24146) 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
           (4-coumaroyl-CoA synthase 2)
          Length = 542

 Score =  104 bits (259), Expect = 8e-22
 Identities = 113/440 (25%), Positives = 185/440 (41%), Gaps = 84/440 (19%)

Query: 131 TYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSSI 190
           TY  +E      A GL   G+ P + I +   NS  ++ A  G    GAI+ +     + 
Sbjct: 54  TYADVELNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTP 113

Query: 191 EELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVILLWGEKSCLVNEGSKEVPIF 250
            E+++    S S  + V      N++ K +  +  ++ + +    + CL         + 
Sbjct: 114 AEVVKQAKAS-SAKIIVTQACHVNKV-KDYAFENDVKIICIDSAPEGCL------HFSVL 165

Query: 251 TFTEIMHLGRGSRRLFESHDARKHYVFEA-IKSDDIATLVYTSGTTGNPKGVMLTHQNLL 309
           T                   A +H + E  I+ DD+  L Y+SGTTG PKGVMLTH+ L+
Sbjct: 166 T------------------QANEHDIPEVEIQPDDVVALPYSSGTTGLPKGVMLTHKGLV 207

Query: 310 HQISK---------YAASLACI*KSL*VFHFFMRC*PSIYNCEKLEVYESLYSGIQRQIS 360
             +++         Y  S   +   L +FH        IY+   +     L  G+ R  +
Sbjct: 208 TSVAQQVDGENPNLYIHSEDVMLCVLPLFH--------IYSLNSV-----LLCGL-RVGA 253

Query: 361 TSSLVRKLVALTFIRVSLGYMECKRIYEGKCLTKNQKSPSYLYAMLDWLGARIIATILFP 420
              +++K   ++F+ +   Y      +    +    KSP     M+D      + T++  
Sbjct: 254 AILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSP-----MVDDYDLSSVRTVMSG 308

Query: 421 IHMLAKKLVYSKIHSAIGFSKAGISGGGSLPSHVDRFFEAIGVTLQNGYGLTETSPVIA- 479
              L K+L    + +    +K G                        GYG+TE  PV+A 
Sbjct: 309 AAPLGKEL-EDTVRAKFPNAKLG-----------------------QGYGMTEAGPVLAM 344

Query: 480 ----ARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPS 535
               A+       G+ G  +++ E K+VD +TG  LP    G + +RG Q+MKGY  +P 
Sbjct: 345 CLAFAKEPFEIKSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPE 404

Query: 536 ATNQAIDKDGWLNTGDIGWI 555
           AT + IDK+GWL TGDIG+I
Sbjct: 405 ATARTIDKEGWLYTGDIGYI 424


>4CL_PINTA (P41636) 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL)
           (4-coumaroyl-CoA synthase)
          Length = 537

 Score =  101 bits (251), Expect = 7e-21
 Identities = 122/465 (26%), Positives = 186/465 (39%), Gaps = 98/465 (21%)

Query: 112 KYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVAD 171
           ++ D+  LID   D   T  + ++E      A GL  +G+   + + L   N   +    
Sbjct: 37  EFADRPCLIDGATD--RTYCFSEVELISRKVAAGLAKLGLQQGQVVMLLLPNCIEFAFVF 94

Query: 172 QGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVIL 231
            G    GAI                     + A     P    + AKA    A  R ++ 
Sbjct: 95  MGASVRGAI--------------------VTTANPFYKPGEIAKQAKA----AGARIIVT 130

Query: 232 LWGEKSCLVNEGSKEVPIFTFTEIMHLGRGSRRLFESHDARKHYVFEAIKSDDIATLVYT 291
           L      L +  S +V + T  +    G     +    D  +    + I  DD+  L Y+
Sbjct: 131 LAAYVEKLADLQSHDVLVITIDDAPKEGCQHISVLTEADETQCPAVK-IHPDDVVALPYS 189

Query: 292 SGTTGNPKGVMLTHQNLLHQISK---------YAASLACI*KSL*VFHFFMRC*PSIYNC 342
           SGTTG PKGVMLTH+ L+  +++         Y  S   I   L +FH +          
Sbjct: 190 SGTTGLPKGVMLTHKGLVSSVAQQVDGENPNLYFHSDDVILCVLPLFHIY---------- 239

Query: 343 EKLEVYESLYSGIQRQISTSSLVRKLVALTFIRVSLGYMECKRIYEGKCLTKNQKSPSYL 402
                  SL S               V L  +R     +  ++     CL   QK     
Sbjct: 240 -------SLNS---------------VLLCALRAGAATLIMQKFNLTTCLELIQK----- 272

Query: 403 YAMLDWLGARIIATILFPIHMLAKKLVYSKIHSAIGFSKAGISGGGSLP-------SHVD 455
           Y +        +A I+ PI +   K   S I S    S   I   G+ P       +  +
Sbjct: 273 YKVT-------VAPIVPPIVLDITK---SPIVSQYDVSSVRIIMSGAAPLGKELEDALRE 322

Query: 456 RFFEAIGVTLQNGYGLTETSPVIA-----ARRLSCNVIGSVGHPLKHTEFKVVDSETGEV 510
           RF +AI      GYG+TE  PV+A     A+       GS G  +++ + K++D+ETGE 
Sbjct: 323 RFPKAI---FGQGYGMTEAGPVLAMNLAFAKNPFPVKSGSCGTVVRNAQIKILDTETGES 379

Query: 511 LPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
           LP    G + +RGP++MKGY  +P +T   ID++GWL+TGD+ +I
Sbjct: 380 LPHNQAGEICIRGPEIMKGYINDPESTAATIDEEGWLHTGDVEYI 424


>LUCI_LUCLA (Q01158) Luciferin 4-monooxygenase (EC 1.13.12.7)
           (Luciferase)
          Length = 548

 Score = 96.7 bits (239), Expect = 2e-19
 Identities = 78/281 (27%), Positives = 129/281 (45%), Gaps = 44/281 (15%)

Query: 278 EAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAASLACI*KSL*VFHFFMRC*P 337
           E  + + +A ++ +SG+TG PKGV LTH+N + + S                        
Sbjct: 187 EVNRKEQVALIMNSSGSTGLPKGVQLTHENAVTRFS------------------------ 222

Query: 338 SIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTF---IRVSLGYMECKRIYEGKCLTK 394
                      + +Y     Q+S  + +  +V       +  +LGY+ C   +    LTK
Sbjct: 223 --------HARDPIYGN---QVSPGTAILTVVPFHHGFGMFTTLGYLTCG--FRIVMLTK 269

Query: 395 NQKSPSYLYAMLDW-LGARIIATILFPIHMLAKKLVYSKIHSAIGFSKAGISGGGSLPSH 453
             +  ++L  + D+   + I+   LF I   ++ L    + + +  +  G      +   
Sbjct: 270 FDEE-TFLKTLQDYKCSSVILVPTLFAILNRSELLDKYDLSNLVEIASGGAPLSKEIGEA 328

Query: 454 VDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPP 513
           V R F   GV  + GYGLTET+  I       +  G+ G  +   + KV+D +T + L P
Sbjct: 329 VARRFNLPGV--RQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKTLGP 386

Query: 514 GYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGW 554
             +G + V+GP LMKGY  NP AT + ID++GWL+TGDIG+
Sbjct: 387 NRRGEVCVKGPMLMKGYVDNPEATREIIDEEGWLHTGDIGY 427


>LUCI_PHOPY (P08659) Luciferin 4-monooxygenase (EC 1.13.12.7)
           (Luciferase)
          Length = 550

 Score = 96.3 bits (238), Expect = 2e-19
 Identities = 114/438 (26%), Positives = 182/438 (41%), Gaps = 76/438 (17%)

Query: 130 MTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSS 189
           +TY +  +  +  AE ++  G++ N +I + ++NS ++ +   G +  G          +
Sbjct: 51  ITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYN 110

Query: 190 IEELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVILLWGEKSCLVNEGSKEVPI 249
             ELL   N S+   + V    +   +     L    + +I+     S    +G + +  
Sbjct: 111 ERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIM----DSKTDYQGFQSMYT 166

Query: 250 FTFTEIMHLGRGSRRLFESHDARKHYVFEAIKSDD-IATLVYTSGTTGNPKGVMLTHQNL 308
           F  +   HL  G    F  +D    +V E+   D  IA ++ +SG+TG PKGV L H+  
Sbjct: 167 FVTS---HLPPG----FNEYD----FVPESFDRDKTIALIMNSSGSTGLPKGVALPHRT- 214

Query: 309 LHQISKYAASLACI*KSL*VFHFFMRC*PSIYNCEKLEVYESLYSGIQRQISTSSLVRKL 368
                      AC+         F      I+                 QI   + +  +
Sbjct: 215 -----------ACV--------RFSHARDPIFG---------------NQIIPDTAILSV 240

Query: 369 VALTF---IRVSLGYMECK-RIYEGKCLTKNQKSPSYLYAMLDWL--GARIIATIL--FP 420
           V       +  +LGY+ C  R+     L    +   +L ++ D+    A ++ T+   F 
Sbjct: 241 VPFHHGFGMFTTLGYLICGFRVV----LMYRFEEELFLRSLQDYKIQSALLVPTLFSFFA 296

Query: 421 IHMLAKKLVYSKIHSAIGFSKAGISGGGSLPSHVD----RFFEAIGVTLQNGYGLTETSP 476
              L  K   S +H          SGG  L   V     + F   G+  + GYGLTET+ 
Sbjct: 297 KSTLIDKYDLSNLHEIA-------SGGAPLSKEVGEAVAKRFHLPGI--RQGYGLTETTS 347

Query: 477 VIAARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSA 536
            I       +  G+VG  +   E KVVD +TG+ L    +G L VRGP +M GY  NP A
Sbjct: 348 AILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEA 407

Query: 537 TNQAIDKDGWLNTGDIGW 554
           TN  IDKDGWL++GDI +
Sbjct: 408 TNALIDKDGWLHSGDIAY 425


>LUCI_LUCCR (P13129) Luciferin 4-monooxygenase (EC 1.13.12.7)
           (Luciferase)
          Length = 548

 Score = 95.1 bits (235), Expect = 5e-19
 Identities = 79/281 (28%), Positives = 128/281 (45%), Gaps = 44/281 (15%)

Query: 278 EAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAASLACI*KSL*VFHFFMRC*P 337
           E  + + +A ++ +SG+TG PKGV LTH+N + + S                        
Sbjct: 187 EVDRKEQVALIMNSSGSTGLPKGVQLTHENTVTRFS------------------------ 222

Query: 338 SIYNCEKLEVYESLYSGIQRQISTSSLVRKLVALTF---IRVSLGYMECKRIYEGKCLTK 394
                      + +Y     Q+S  + V  +V       +  +LGY+ C   +    LTK
Sbjct: 223 --------HARDPIYGN---QVSPGTAVLTVVPFHHGFGMFTTLGYLICG--FRVVMLTK 269

Query: 395 NQKSPSYLYAMLDWLGARIIAT-ILFPIHMLAKKLVYSKIHSAIGFSKAGISGGGSLPSH 453
             +  ++L  + D+    +I    LF I   ++ L    + + +  +  G      +   
Sbjct: 270 FDEE-TFLKTLQDYKCTSVILVPTLFAILNKSELLNKYDLSNLVEIASGGAPLSKEVGEA 328

Query: 454 VDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPP 513
           V R F   GV  + GYGLTET+  I       +  G+ G  +   + KV+D +T + L P
Sbjct: 329 VARRFNLPGV--RQGYGLTETTSAIIITPEGDDKPGASGKVVPLFKAKVIDLDTKKSLGP 386

Query: 514 GYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGW 554
             +G + V+GP LMKGY  NP AT + ID++GWL+TGDIG+
Sbjct: 387 NRRGEVCVKGPMLMKGYVNNPEATKELIDEEGWLHTGDIGY 427


>4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1)
          Length = 561

 Score = 87.8 bits (216), Expect = 8e-17
 Identities = 82/295 (27%), Positives = 132/295 (43%), Gaps = 67/295 (22%)

Query: 280 IKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISK---------YAASLACI*KSL*VFH 330
           I  DD+  L Y+SGTTG PKGVMLTH+ L+  +++         Y  S   I   L +FH
Sbjct: 199 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 258

Query: 331 FF---------MRC*PSIYNCEKLEVYESLYSGIQR-QISTSSLVRKLVALTFIRVSLGY 380
            +         +R   +I    K E+   L   IQR +++ + +V  +V           
Sbjct: 259 IYALNSIMLCGLRVGAAILIMPKFEI-NLLLELIQRCKVTVAPMVPPIVL---------- 307

Query: 381 MECKRIYEGKCLTKNQKSPSYLYAMLDWLGARIIATILFPIHMLAKKLVYSKIHSAIGFS 440
                      + K+ ++  Y     D    R++ +   P+    +  V +K  +A    
Sbjct: 308 ----------AIAKSSETEKY-----DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQ 352

Query: 441 KAGISGGGSLPSHVDRFFEAIGVTLQNGYGLTETSPVIAARRLSCNVIGSVGHPLKHTEF 500
             G++  G +            + +  G+   E  PV +    +C  +      +++ E 
Sbjct: 353 GYGMTEAGPV------------LAMSLGFA-KEPFPVKSG---ACGTV------VRNAEM 390

Query: 501 KVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
           K+VD +TG+ L     G + +RG Q+MKGY  NP+AT + IDKDGWL+TGDIG I
Sbjct: 391 KIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGDIGLI 445


>LCFA_BACSU (P94547) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
           (Long-chain acyl-CoA synthetase)
          Length = 560

 Score = 87.4 bits (215), Expect = 1e-16
 Identities = 51/124 (41%), Positives = 72/124 (57%), Gaps = 3/124 (2%)

Query: 434 HSAIGFSKAGISGGGSLPSHVDRFFEAI-GVTLQNGYGLTETSPVIAARRL-SCNVIGSV 491
           H  +   K+ +SG  +LP  V + FE + G  L  GYGL+E SPV  A  +   N  GS+
Sbjct: 319 HYDLSSIKSCLSGSAALPVEVKQKFEKVTGGKLVEGYGLSEASPVTHANFIWGKNKPGSI 378

Query: 492 GHPLKHTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGD 551
           G P   T+  +   ETGE+  P   G + V+GPQ+MKGY+  P  T  A+ +DGWL TGD
Sbjct: 379 GCPWPSTDAAIYSEETGELAAPYEHGEIIVKGPQVMKGYWNKPEET-AAVLRDGWLFTGD 437

Query: 552 IGWI 555
           +G++
Sbjct: 438 MGYM 441



 Score = 52.4 bits (124), Expect = 4e-06
 Identities = 57/232 (24%), Positives = 96/232 (40%), Gaps = 29/232 (12%)

Query: 100 KTVPDIWRSSAEKYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRVIGVSPNEKIAL 159
           KT+  I   SA ++ DK A+   ++     +T+  +    L  A  L+  G+   +++A+
Sbjct: 24  KTLQSILTDSAARFPDKTAI--SFYG--KKLTFHDILTDALKLAAFLQCNGLQKGDRVAV 79

Query: 160 FADNSCRWLVADQGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNRIAKA 219
              N  + +++  G++  G I VV+ +    E  L+       +++ +    +F +  K 
Sbjct: 80  MLPNCPQTVISYYGVLFAGGI-VVQTNPLYTEHELEYQLRDAQVSVIITLDLLFPKAIKM 138

Query: 220 FDLKASMRFVI------------LLWG--EKSCLVNEGSKEVPIFTFTEIMHLGRGSRRL 265
             L    + +I            +L+   +K  +  +  K   I TF   M   +     
Sbjct: 139 KTLSIVDQILITSVKDYLPFPKNILYPLTQKQKVHIDFDKTANIHTFASCMKQEKTELLT 198

Query: 266 FESHDARKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISKYAA 317
               D             DIA L YT GTTG PKGVMLTHQN+L      AA
Sbjct: 199 IPKIDPEH----------DIAVLQYTGGTTGAPKGVMLTHQNILANTEMCAA 240


>4CL_VANPL (O24540) 4-coumarate--CoA ligase (EC 6.2.1.12) (4CL)
           (4-coumaroyl-CoA synthase)
          Length = 553

 Score = 87.4 bits (215), Expect = 1e-16
 Identities = 44/96 (45%), Positives = 61/96 (62%), Gaps = 5/96 (5%)

Query: 465 LQNGYGLTETSPVIA-----ARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPPGYKGIL 519
           L  GYG+TE  PV+A     A+       G+ G  +++ E K+VD ETG  LP  + G +
Sbjct: 338 LGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEI 397

Query: 520 KVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
            +RG Q+MKGY  +P AT + IDK+GWL+TGDIG+I
Sbjct: 398 CIRGDQIMKGYLNDPEATARTIDKEGWLHTGDIGYI 433



 Score = 42.4 bits (98), Expect = 0.004
 Identities = 50/209 (23%), Positives = 80/209 (37%), Gaps = 34/209 (16%)

Query: 112 KYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVAD 171
           K+  +  LI+   D     TY  +E        GL  +G+   + I +   NS  ++ A 
Sbjct: 41  KFSSRPCLINGATD--EIFTYADVELISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAF 98

Query: 172 QGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVIL 231
            G    G+I+ +     +  E++                    + AKA + K  +     
Sbjct: 99  LGASFIGSISTMANPFFTSTEVI--------------------KQAKASNAKLIITQGCY 138

Query: 232 LWGEKSCLVNEGSKEVPIFTFT------EIMHLGRGSRRLFESHDARKHYVFEAIKSDDI 285
           +   K      G K + I T T       I+H            D  +    E I  D +
Sbjct: 139 VDKVKDYACENGVKIISIDTTTTADDAANILHFSE-----LTGADENEMPKVE-ISPDGV 192

Query: 286 ATLVYTSGTTGNPKGVMLTHQNLLHQISK 314
             L Y+SGTTG PKGVMLTH+ L+  +++
Sbjct: 193 VALPYSSGTTGLPKGVMLTHKGLVTSVAQ 221


>4CL1_SOYBN (P31686) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1) (Clone 4CL14) (Fragment)
          Length = 293

 Score = 84.7 bits (208), Expect = 6e-16
 Identities = 48/118 (40%), Positives = 65/118 (54%), Gaps = 7/118 (5%)

Query: 445 SGGGSLPSHVDRFFEAI--GVTLQNGYGLTETSPVIA-----ARRLSCNVIGSVGHPLKH 497
           SGG  L   ++    A      L  GYG+TE  PV+      A+       G+ G  +++
Sbjct: 57  SGGAPLGKELEDTLRAKFPNAKLGQGYGMTEAGPVLTMSLAFAKEPIDVKPGACGTVVRN 116

Query: 498 TEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
            E K+VD ETG  LP    G + +RG Q+MKGY  +  AT + IDKDGWL+TGDIG+I
Sbjct: 117 AEMKIVDPETGHSLPRNQSGEICIRGDQIMKGYLNDGEATERTIDKDGWLHTGDIGYI 174


>4CL1_ORYSA (P17814) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1)
          Length = 563

 Score = 84.7 bits (208), Expect = 6e-16
 Identities = 44/119 (36%), Positives = 68/119 (56%), Gaps = 7/119 (5%)

Query: 444 ISGGGSLPSHVDRFFEAI--GVTLQNGYGLTETSPVIA-----ARRLSCNVIGSVGHPLK 496
           +SG   +   ++  F A   G  L  GYG+TE  PV++     A+       G+ G  ++
Sbjct: 326 LSGAAPMGKDIEDAFMAKLPGAVLGQGYGMTEAGPVLSMCLAFAKEPFKVKSGACGTVVR 385

Query: 497 HTEFKVVDSETGEVLPPGYKGILKVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
           + E K++D +TG+ L    +G + +RG Q+MKGY  NP AT   ID +GWL+TGDIG++
Sbjct: 386 NAELKIIDPDTGKSLGRNLRGEICIRGQQIMKGYLNNPEATKNTIDAEGWLHTGDIGYV 444



 Score = 42.7 bits (99), Expect = 0.003
 Identities = 18/32 (56%), Positives = 25/32 (77%)

Query: 283 DDIATLVYTSGTTGNPKGVMLTHQNLLHQISK 314
           DD+  L Y+SGTTG PKGVMLTH++L   +++
Sbjct: 201 DDVVALPYSSGTTGLPKGVMLTHRSLSTSVAQ 232


>4CL1_TOBAC (O24145) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1)
           (4-coumaroyl-CoA synthase 1)
          Length = 547

 Score = 84.0 bits (206), Expect = 1e-15
 Identities = 43/96 (44%), Positives = 60/96 (61%), Gaps = 5/96 (5%)

Query: 465 LQNGYGLTETSPVIA-----ARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPPGYKGIL 519
           L  GYG+TE  PV+A     A+       G+ G  +++ E K+VD +TG  LP    G +
Sbjct: 334 LGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEI 393

Query: 520 KVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
            +RG Q+MKGY  +P AT + IDK+GWL+TGDIG+I
Sbjct: 394 CIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIGFI 429



 Score = 52.0 bits (123), Expect = 5e-06
 Identities = 45/185 (24%), Positives = 80/185 (42%), Gaps = 27/185 (14%)

Query: 131 TYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVADQGMMATGAINVVRGSRSSI 190
           TY ++E      A GL  +G+   + I +   NS  ++ A  G    GAI+ +     + 
Sbjct: 59  TYAEVELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTP 118

Query: 191 EELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVILLWGEKSCLVNEGSKEVPIF 250
            E+++      S A  +     F    K +  +  ++ + +    + CL           
Sbjct: 119 AEVVK--QAKASSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCL----------- 165

Query: 251 TFTEIMHLGRGSRRLFESHDARKHYVFEA-IKSDDIATLVYTSGTTGNPKGVMLTHQNLL 309
            F+E+               + +H + E  I+ DD+  L Y+SGTTG PKGVMLTH+ L+
Sbjct: 166 HFSELTQ-------------SDEHEIPEVKIQPDDVVALPYSSGTTGLPKGVMLTHKGLV 212

Query: 310 HQISK 314
             +++
Sbjct: 213 TSVAQ 217


>4CL2_ARATH (Q9S725) 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
           (4-coumaroyl-CoA synthase 2)
          Length = 556

 Score = 83.6 bits (205), Expect = 1e-15
 Identities = 42/96 (43%), Positives = 60/96 (61%), Gaps = 5/96 (5%)

Query: 465 LQNGYGLTETSPVIA-----ARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPPGYKGIL 519
           L  GYG+TE  PV+A     A+       G+ G  +++ E K++D +TG+ LP    G +
Sbjct: 343 LGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKILDPDTGDSLPRNKPGEI 402

Query: 520 KVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
            +RG Q+MKGY  +P AT   IDKDGWL+TGD+G+I
Sbjct: 403 CIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 438



 Score = 46.2 bits (108), Expect = 3e-04
 Identities = 21/50 (42%), Positives = 33/50 (66%)

Query: 265 LFESHDARKHYVFEAIKSDDIATLVYTSGTTGNPKGVMLTHQNLLHQISK 314
           L +S + R   + E I  +D+  L ++SGTTG PKGVMLTH+ L+  +++
Sbjct: 177 LTQSEEPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQ 226


>4CL2_SOLTU (P31685) 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
           (4-coumaroyl-CoA synthase 2)
          Length = 545

 Score = 81.6 bits (200), Expect = 5e-15
 Identities = 42/96 (43%), Positives = 60/96 (61%), Gaps = 5/96 (5%)

Query: 465 LQNGYGLTETSPVIA-----ARRLSCNVIGSVGHPLKHTEFKVVDSETGEVLPPGYKGIL 519
           L  GYG+TE  PV+A     A+       G+ G  +++ E K+VD +TG  LP    G +
Sbjct: 332 LGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEI 391

Query: 520 KVRGPQLMKGYYKNPSATNQAIDKDGWLNTGDIGWI 555
            +RG Q+MKGY  +P AT + I+K+GWL+TGDIG+I
Sbjct: 392 CIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFI 427



 Score = 50.4 bits (119), Expect = 1e-05
 Identities = 48/203 (23%), Positives = 87/203 (42%), Gaps = 27/203 (13%)

Query: 112 KYGDKIALIDPYHDPPSTMTYKQLEDAILDFAEGLRVIGVSPNEKIALFADNSCRWLVAD 171
           ++  +  LID  +D     TY ++E      A GL  +G+   + I +   N   ++ A 
Sbjct: 40  EFNSRPCLIDGAND--RIYTYAEVELTSRKVAVGLNKLGIQQKDTIMILLPNCPEFVFAF 97

Query: 172 QGMMATGAINVVRGSRSSIEELLQIYNHSESIALAVDNPEMFNRIAKAFDLKASMRFVIL 231
            G    GAI+ +     +  E+++      S A  V     F    K + ++  ++ + +
Sbjct: 98  IGASYLGAISTMANPLFTPAEVVK--QAKASSAKIVITQACFAGKVKDYAIENDLKVICV 155

Query: 232 LWGEKSCLVNEGSKEVPIFTFTEIMHLGRGSRRLFESHDARKHYVFEAIKSDDIATLVYT 291
               + C+            F+E++          E  D +       I+ DD+  L Y+
Sbjct: 156 DSAPEGCV-----------HFSELIQSDE-----HEIPDVK-------IQPDDVVALPYS 192

Query: 292 SGTTGNPKGVMLTHQNLLHQISK 314
           SGTTG PKGVMLTH+ L+  +++
Sbjct: 193 SGTTGLPKGVMLTHKGLVTSVAQ 215


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.326    0.140    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,313,141
Number of Sequences: 164201
Number of extensions: 2985570
Number of successful extensions: 9258
Number of sequences better than 10.0: 216
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 29
Number of HSP's that attempted gapping in prelim test: 8603
Number of HSP's gapped (non-prelim): 544
length of query: 617
length of database: 59,974,054
effective HSP length: 116
effective length of query: 501
effective length of database: 40,926,738
effective search space: 20504295738
effective search space used: 20504295738
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 69 (31.2 bits)