BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146683.3 + phase: 0 /pseudo
(122 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
S23A_MOUSE (Q01405) Protein transport protein Sec23A (SEC23-rela... 52 2e-07
S23A_HUMAN (Q15436) Protein transport protein Sec23A (SEC23-rela... 52 2e-07
S23B_MOUSE (Q9D662) Protein transport protein Sec23B (SEC23-rela... 49 2e-06
S23B_HUMAN (Q15437) Protein transport protein Sec23B (SEC23-rela... 49 3e-06
SC23_YEAST (P15303) Protein transport protein SEC23 45 4e-05
PT15_STYPL (P28207) Protein-tyrosine phosphatase 15 (EC 3.1.3.48... 30 0.96
NU5M_CERSI (O03205) NADH-ubiquinone oxidoreductase chain 5 (EC 1... 30 0.96
NM3B_HUMAN (O60391) Glutamate [NMDA] receptor subunit 3B precurs... 30 1.3
CD69_HUMAN (Q07108) Early activation antigen CD69 (Early T-cell ... 29 1.6
NU5M_RHIUN (Q96069) NADH-ubiquinone oxidoreductase chain 5 (EC 1... 28 4.8
NPRM_BACME (Q00891) Bacillolysin precursor (EC 3.4.24.28) (Neutr... 27 6.2
ZIC3_XENLA (O57311) Zinc finger protein ZIC 3 (Zinc finger prote... 27 8.1
ZF26_MOUSE (P10076) Zinc finger protein 26 (Zfp-26) (mKR3 protei... 27 8.1
POL_CERV (P05400) Enzymatic polyprotein [Contains: Aspartic prot... 27 8.1
A85B_MYCKA (P21160) Antigen 85-B precursor (85B) (Extracellular ... 27 8.1
>S23A_MOUSE (Q01405) Protein transport protein Sec23A (SEC23-related
protein A)
Length = 765
Score = 52.4 bits (124), Expect = 2e-07
Identities = 27/55 (49%), Positives = 37/55 (67%), Gaps = 5/55 (9%)
Query: 50 RSTGVAFSAALGLLECCFVNTGSRIMVF-----ASGPGLVLDSDFWQSMRTHNDI 99
RS+GVA S A+GLLEC F NTG+RIM+F GPG+V+ + +R+ +DI
Sbjct: 262 RSSGVALSIAVGLLECTFPNTGARIMMFIGGPATQGPGMVVGDELKTPIRSWHDI 316
>S23A_HUMAN (Q15436) Protein transport protein Sec23A (SEC23-related
protein A)
Length = 765
Score = 52.4 bits (124), Expect = 2e-07
Identities = 27/55 (49%), Positives = 37/55 (67%), Gaps = 5/55 (9%)
Query: 50 RSTGVAFSAALGLLECCFVNTGSRIMVF-----ASGPGLVLDSDFWQSMRTHNDI 99
RS+GVA S A+GLLEC F NTG+RIM+F GPG+V+ + +R+ +DI
Sbjct: 262 RSSGVALSIAVGLLECTFPNTGARIMMFIGGPATQGPGMVVGDELKTPIRSWHDI 316
>S23B_MOUSE (Q9D662) Protein transport protein Sec23B (SEC23-related
protein B)
Length = 767
Score = 49.3 bits (116), Expect = 2e-06
Identities = 27/55 (49%), Positives = 36/55 (65%), Gaps = 5/55 (9%)
Query: 50 RSTGVAFSAALGLLECCFVNTGSRIMVF-----ASGPGLVLDSDFWQSMRTHNDI 99
RSTGVA S A+GLLE F NTG+RIM+F GPG+V+ + +R+ +DI
Sbjct: 264 RSTGVALSIAVGLLEGTFPNTGARIMLFTGGPPTQGPGMVVGDELKTPIRSWHDI 318
>S23B_HUMAN (Q15437) Protein transport protein Sec23B (SEC23-related
protein B)
Length = 767
Score = 48.5 bits (114), Expect = 3e-06
Identities = 27/55 (49%), Positives = 36/55 (65%), Gaps = 5/55 (9%)
Query: 50 RSTGVAFSAALGLLECCFVNTGSRIMVF-----ASGPGLVLDSDFWQSMRTHNDI 99
RSTGVA S A+GLLE F NTG+RIM+F GPG+V+ + +R+ +DI
Sbjct: 264 RSTGVALSIAVGLLEGTFPNTGARIMLFTGGPPTQGPGMVVGDELKIPIRSWHDI 318
>SC23_YEAST (P15303) Protein transport protein SEC23
Length = 768
Score = 44.7 bits (104), Expect = 4e-05
Identities = 23/55 (41%), Positives = 37/55 (66%), Gaps = 5/55 (9%)
Query: 50 RSTGVAFSAALGLLECCFVNTGSRIMVFASG-----PGLVLDSDFWQSMRTHNDI 99
R+TG A + A LL+ C+ N +RI++FASG PGL+++S+ +R+H+DI
Sbjct: 260 RATGSALNIASLLLQGCYKNIPARIILFASGPGTVAPGLIVNSELKDPLRSHHDI 314
>PT15_STYPL (P28207) Protein-tyrosine phosphatase 15 (EC 3.1.3.48)
(Fragment)
Length = 113
Score = 30.0 bits (66), Expect = 0.96
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 6/47 (12%)
Query: 8 GSIAYFLSTLHYTTWTNTACSKAGITVAIEDIHSTYNFKSRN-RSTG 53
G+I+ ++ LHYT W +T+C + G D+ N N RSTG
Sbjct: 66 GAISRTVTQLHYTGWNSTSCPEDG-----RDVIELVNKMQENIRSTG 107
>NU5M_CERSI (O03205) NADH-ubiquinone oxidoreductase chain 5 (EC
1.6.5.3)
Length = 606
Score = 30.0 bits (66), Expect = 0.96
Identities = 17/50 (34%), Positives = 25/50 (50%)
Query: 18 HYTTWTNTACSKAGITVAIEDIHSTYNFKSRNRSTGVAFSAALGLLECCF 67
HY T A + G +A+E +TYN K ++ S+ + FS LG F
Sbjct: 484 HYLKLTALAVTILGFMMALELSLATYNLKLKHPSSPLKFSNLLGYFPTIF 533
>NM3B_HUMAN (O60391) Glutamate [NMDA] receptor subunit 3B precursor
(N-methyl-D-aspartate receptor subtype NR3B) (NR3B)
(NMDAR3B) (Fragment)
Length = 901
Score = 29.6 bits (65), Expect = 1.3
Identities = 18/57 (31%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Query: 10 IAYFLSTLHYTTWTNT--ACSKAGITVAIEDIHSTYNF--KSRNRSTGVAFSAALGL 62
I F+ LH++TW A + + + + S Y + RNRST ++S+AL L
Sbjct: 566 IGAFMWPLHWSTWLGVFAALHLTALFLTVYEWRSPYGLTPRGRNRSTVFSYSSALNL 622
>CD69_HUMAN (Q07108) Early activation antigen CD69 (Early T-cell
activation antigen p60) (GP32/28) (Leu-23) (MLR-3) (EA1)
(BL-AC/P26) (Activation inducer molecule) (AIM)
Length = 199
Score = 29.3 bits (64), Expect = 1.6
Identities = 16/39 (41%), Positives = 23/39 (58%), Gaps = 3/39 (7%)
Query: 12 YFLSTLHYTTWTNT--ACSKAGITVAIEDIHSTYNFKSR 48
YF+ST+ +WT+ ACS+ G T+A+ D NF R
Sbjct: 97 YFISTVK-RSWTSAQNACSEHGATLAVIDSEKDMNFLKR 134
>NU5M_RHIUN (Q96069) NADH-ubiquinone oxidoreductase chain 5 (EC
1.6.5.3)
Length = 606
Score = 27.7 bits (60), Expect = 4.8
Identities = 19/59 (32%), Positives = 29/59 (48%), Gaps = 2/59 (3%)
Query: 31 GITVAIEDIHSTYNFKSRNRSTGVAFSAALGLLECCFVNTGSRIMVFAS--GPGLVLDS 87
G +A+E +TYN K ++ S + FS+ LG F + + AS L+LDS
Sbjct: 497 GFALALELSLATYNLKFKHPSNPLKFSSLLGYFPTIFHRLPPSMGLLASQKSASLLLDS 555
>NPRM_BACME (Q00891) Bacillolysin precursor (EC 3.4.24.28) (Neutral
protease)
Length = 562
Score = 27.3 bits (59), Expect = 6.2
Identities = 12/38 (31%), Positives = 23/38 (59%)
Query: 5 NKCGSIAYFLSTLHYTTWTNTACSKAGITVAIEDIHST 42
+K G I Y +TL++T T + ++AG+ A D++ +
Sbjct: 507 DKLGKIYYRANTLYFTQSTTFSQARAGLVQAAADLYGS 544
>ZIC3_XENLA (O57311) Zinc finger protein ZIC 3 (Zinc finger protein
of the cerebellum 3) (ZIC3 protein)
Length = 441
Score = 26.9 bits (58), Expect = 8.1
Identities = 13/32 (40%), Positives = 20/32 (61%)
Query: 40 HSTYNFKSRNRSTGVAFSAALGLLECCFVNTG 71
+ST +F RNR++G+A S++ G F N G
Sbjct: 92 NSTRDFLFRNRNSGIADSSSAGSQHGLFANHG 123
>ZF26_MOUSE (P10076) Zinc finger protein 26 (Zfp-26) (mKR3 protein)
(Fragment)
Length = 428
Score = 26.9 bits (58), Expect = 8.1
Identities = 16/48 (33%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Query: 50 RSTGVAFSAALGLLECCFVNTGSR-IMVFASGPGLVLDSDFWQSMRTH 96
+ G AFS + GL+E ++TG + + G LV S +RTH
Sbjct: 69 KECGKAFSTSSGLVEHIRIHTGEKPFECYQCGKALVHSSSLVGHLRTH 116
>POL_CERV (P05400) Enzymatic polyprotein [Contains: Aspartic
protease (EC 3.4.23.-); Endonuclease; Reverse
transcriptase (EC 2.7.7.49)]
Length = 659
Score = 26.9 bits (58), Expect = 8.1
Identities = 13/45 (28%), Positives = 24/45 (52%), Gaps = 3/45 (6%)
Query: 66 CFVNTGSRIMVFASGPGLVLDSDFWQSMRTHNDIYICNGHLAVYT 110
C+V+TGS + + + V+ ++WQ+ +I I NG + T
Sbjct: 31 CYVDTGSSLCMASK---YVIPEEYWQTAEKPLNIKIANGKIIQLT 72
>A85B_MYCKA (P21160) Antigen 85-B precursor (85B) (Extracellular
alpha-antigen) (Antigen 85 complex B) (Ag85B) (Mycolyl
transferase 85B) (EC 2.3.1.-) (Fibronectin-binding
protein B)
Length = 325
Score = 26.9 bits (58), Expect = 8.1
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 3/47 (6%)
Query: 19 YTTWTNTACSKAGITVAIEDIHSTY---NFKSRNRSTGVAFSAALGL 62
Y+ W + AC KAG T + T + S NRS SAA+G+
Sbjct: 119 YSDWYSPACGKAGCTTYKWETFLTSELPQWLSANRSVKPTGSAAVGI 165
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.135 0.441
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,907,600
Number of Sequences: 164201
Number of extensions: 491431
Number of successful extensions: 1172
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1162
Number of HSP's gapped (non-prelim): 15
length of query: 122
length of database: 59,974,054
effective HSP length: 98
effective length of query: 24
effective length of database: 43,882,356
effective search space: 1053176544
effective search space used: 1053176544
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC146683.3