BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146651.18 + phase: 0 /pseudo
(559 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TRA0_MYCSM (P35883) Transposase for insertion sequence element I... 39 0.047
TRA5_LACLA (P35881) Transposase for insertion sequence element I... 36 0.30
GLYG_HUMAN (P46976) Glycogenin-1 (EC 2.4.1.186) 33 2.6
KAD5_SCHPO (Q09831) Probable serine/threonine-protein kinase C4G... 32 4.4
YPS1_YEAST (P32329) Aspartic proteinase 3 precursor (EC 3.4.23.4... 31 7.5
VNUA_PRVKA (P33485) Probable nuclear antigen 31 7.5
CCAH_MOUSE (O88427) Voltage-dependent T-type calcium channel alp... 31 7.5
YJD1_SCHPO (O74504) Hypothetical protein C594.01 in chromosome III 31 9.7
YCZ7_SCHPO (O74560) Hypothetical protein C970.07c in chromosome III 31 9.7
POT3_ARATH (Q9FE38) Potassium transporter 3 (AtPOT3) (AtKUP4) (A... 31 9.7
PC_DROME (P26017) Polycomb protein 31 9.7
DBF2_YEAST (P22204) Cell cycle protein kinase DBF2 (EC 2.7.1.37) 31 9.7
>TRA0_MYCSM (P35883) Transposase for insertion sequence element
IS6120
Length = 323
Score = 38.5 bits (88), Expect = 0.047
Identities = 38/152 (25%), Positives = 58/152 (38%), Gaps = 19/152 (12%)
Query: 137 ILRKLLTSKMNMPKVIVTDRDMSLMKAVENIFPKSYTMNCYFHVQANVKQRCVLDFKYPL 196
+LR M P + + D + KAV +FP + C+FH QANV + +P
Sbjct: 113 LLRDCKRRGMTAPVLAIGDGALGFWKAVREVFPATKEQRCWFHKQANV-LAALPKSAHPS 171
Query: 197 GKRDGKEVKPRDVVKKIMRA*KAMVELPTQELYANASVEFQNSCSDFSLFKNYAMTTLNE 256
KE+ + + K A KA E Y A + + F Y
Sbjct: 172 ALAAIKEIYNAEDIDKAQIAVKAF-EADFGAKYPKAVAKITDDLDVLLEFYKYP------ 224
Query: 257 VKEKIVRAWTDHVLHLGCRTSNMVESAHALLK 288
+H +HL RT+N +ES A ++
Sbjct: 225 ---------AEHWIHL--RTTNPIESTFATVR 245
>TRA5_LACLA (P35881) Transposase for insertion sequence element
IS905
Length = 391
Score = 35.8 bits (81), Expect = 0.30
Identities = 30/152 (19%), Positives = 65/152 (42%), Gaps = 10/152 (6%)
Query: 40 KSLQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTY----K 95
+ + +I ++ H+Y+ + + T E++ H ++ N+ +VL +D TY +
Sbjct: 115 REISDIIERMYGHHYSPATISNISKATQENVATFHERSLEA--NY-SVLFLDGTYLPLRR 171
Query: 96 TNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRKLLTSKMNMPKVIVTD 155
+ K + +G+T +G+ +E N W +L KL + ++VTD
Sbjct: 172 GTVSKECIHIALGITPEGQKAVLGYEIAPNEN--NASWST-LLDKLQNQGIQQVSLVVTD 228
Query: 156 RDMSLMKAVENIFPKSYTMNCYFHVQANVKQR 187
L + + +P + C H+ N+ +
Sbjct: 229 GFKGLEQIISQAYPLAKQQRCLIHISRNLASK 260
>GLYG_HUMAN (P46976) Glycogenin-1 (EC 2.4.1.186)
Length = 349
Score = 32.7 bits (73), Expect = 2.6
Identities = 25/93 (26%), Positives = 42/93 (44%), Gaps = 2/93 (2%)
Query: 37 GDKKSLQYLISKLEEHNYTYYSRTQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKT 96
G + + + +++ NYTY +T+S + D HP F+ L+ N T V+ +
Sbjct: 204 GASAKVVHFLGRVKPWNYTYDPKTKSVKSEAHDPNMTHPEFLILWWNIFTTNVLPLLQQF 263
Query: 97 NMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEE 129
+ K V V S DL Y++ F KE+
Sbjct: 264 GLVKDTC-SYVNVLS-DLVYTLAFSCGFCRKED 294
>KAD5_SCHPO (Q09831) Probable serine/threonine-protein kinase
C4G8.05 (EC 2.7.1.37)
Length = 566
Score = 32.0 bits (71), Expect = 4.4
Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 6/96 (6%)
Query: 60 TQSESTTIEDIFWAHPTFVKLFNNFPTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVG 119
T + ++DIF H +L + TV TY +N K+ E VG +S + + +G
Sbjct: 144 TSVQREKLKDIFSPHGKEKELAHIKKTVATRARTYSSNSIKICDVE-VGPSSFEKVFLLG 202
Query: 120 FG-----FVTHEKEENFVWVLKILRKLLTSKMNMPK 150
G ++ EK+ + +K+L K K N K
Sbjct: 203 KGDVGRVYLVREKKSGKFYAMKVLSKQEMIKRNKSK 238
>YPS1_YEAST (P32329) Aspartic proteinase 3 precursor (EC 3.4.23.41)
(Yapsin 1)
Length = 569
Score = 31.2 bits (69), Expect = 7.5
Identities = 19/69 (27%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Query: 81 FNNFPTVLVMDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFVWVLKILRK 140
++NFP VL K+N Y + + + + T L FG V H K ++ + I+
Sbjct: 276 YDNFPIVLKNSGAIKSNTYSLYLNDSDAMHGTIL-----FGAVDHSKYTGTLYTIPIVNT 330
Query: 141 LLTSKMNMP 149
L S + P
Sbjct: 331 LSASGFSSP 339
>VNUA_PRVKA (P33485) Probable nuclear antigen
Length = 1733
Score = 31.2 bits (69), Expect = 7.5
Identities = 11/20 (55%), Positives = 12/20 (60%)
Query: 470 RLLIPKIRIVNRHHHQQHHH 489
RL +P HHHQQHHH
Sbjct: 10 RLSLPSTTTTRDHHHQQHHH 29
>CCAH_MOUSE (O88427) Voltage-dependent T-type calcium channel
alpha-1H subunit (Voltage-gated calcium channel alpha
subunit Cav3.2)
Length = 2365
Score = 31.2 bits (69), Expect = 7.5
Identities = 20/86 (23%), Positives = 35/86 (40%), Gaps = 10/86 (11%)
Query: 407 NEVGFCVKVELKA---IVEHIKKLLFQMKLEVKEGLRQLTFSETTMMSPPPRKVPTKGAK 463
+E G C + LK I +K+ ++ + R+ +T+ PR+ P + +
Sbjct: 451 SEPGSCYEELLKYVGHIFRKVKRRSLRLYARWQSRWRKKVDPSSTLHGQGPRRRPRRAGR 510
Query: 464 KKVDIARLLIPKIRIVNRHHHQQHHH 489
+ + L V HHH HHH
Sbjct: 511 RTASVHHL-------VYHHHHHHHHH 529
>YJD1_SCHPO (O74504) Hypothetical protein C594.01 in chromosome III
Length = 791
Score = 30.8 bits (68), Expect = 9.7
Identities = 23/73 (31%), Positives = 32/73 (43%), Gaps = 9/73 (12%)
Query: 426 KLLFQMKLEVKEGLRQLTFSE---------TTMMSPPPRKVPTKGAKKKVDIARLLIPKI 476
KL+ +M L K+ LR + S+ +T + V T+ KK R L I
Sbjct: 414 KLIKRMSLRSKKSLRNDSKSDDVGNSTHRFSTASAASTSAVKTEKEKKMSAPRRSLDKLI 473
Query: 477 RIVNRHHHQQHHH 489
RI + H H HHH
Sbjct: 474 RIGSLHRHHHHHH 486
>YCZ7_SCHPO (O74560) Hypothetical protein C970.07c in chromosome III
Length = 636
Score = 30.8 bits (68), Expect = 9.7
Identities = 25/85 (29%), Positives = 40/85 (46%), Gaps = 12/85 (14%)
Query: 98 MYKMLMFEV-VGVTSTDLTY-------SVGFGFVTHEKEENFVWVLKILRKLLTSKMN-M 148
++ L+F++ G++ +D TY +V F F E+NFV VL KL +
Sbjct: 417 LFHFLLFDIGSGLSGSDYTYEQYINHSAVAFSFTEEIFEKNFVTVLPDFVKLFSISFGYW 476
Query: 149 PKVIVTDRDMSLMKAVENIFPKSYT 173
P D L+K + N +PK Y+
Sbjct: 477 PAFSFYD---ELLKLLRNKYPKVYS 498
>POT3_ARATH (Q9FE38) Potassium transporter 3 (AtPOT3) (AtKUP4)
(AtKT3) (Tiny root hair 1 protein)
Length = 775
Score = 30.8 bits (68), Expect = 9.7
Identities = 19/81 (23%), Positives = 36/81 (43%), Gaps = 2/81 (2%)
Query: 90 MDSTYKTNMYKMLMFEVVGVTSTDLTYSVGFGFVTHEKEENFV--WVLKILRKLLTSKMN 147
MD+ ++ K +F++V ++ Y VG G V ++ FV V+ + L
Sbjct: 694 MDTKFRQPQVKEELFDLVNAKDAEVAYIVGHGHVKAKRNSVFVKQLVVNVAYSFLRKNCR 753
Query: 148 MPKVIVTDRDMSLMKAVENIF 168
P V++ + L+K N +
Sbjct: 754 SPGVMLNIPHICLIKVGMNYY 774
>PC_DROME (P26017) Polycomb protein
Length = 390
Score = 30.8 bits (68), Expect = 9.7
Identities = 12/28 (42%), Positives = 19/28 (67%), Gaps = 2/28 (7%)
Query: 480 NRHHHQQHHHIKEKKDVRLGKTSLNPLS 507
+ HHH HHHIK +++ G+ S +PL+
Sbjct: 134 HHHHHHHHHHIKSERN--SGRRSESPLT 159
>DBF2_YEAST (P22204) Cell cycle protein kinase DBF2 (EC 2.7.1.37)
Length = 572
Score = 30.8 bits (68), Expect = 9.7
Identities = 16/42 (38%), Positives = 26/42 (61%), Gaps = 2/42 (4%)
Query: 119 GFG--FVTHEKEENFVWVLKILRKLLTSKMNMPKVIVTDRDM 158
G+G ++ +K+ V LKIL K L K+N K ++T+RD+
Sbjct: 187 GYGQVYLARKKDTKEVCALKILNKKLLFKLNETKHVLTERDI 228
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.139 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,443,302
Number of Sequences: 164201
Number of extensions: 2552169
Number of successful extensions: 8951
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 8931
Number of HSP's gapped (non-prelim): 22
length of query: 559
length of database: 59,974,054
effective HSP length: 115
effective length of query: 444
effective length of database: 41,090,939
effective search space: 18244376916
effective search space used: 18244376916
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 68 (30.8 bits)
Medicago: description of AC146651.18