BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146632.7 + phase: 0
(339 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ETB2_HUMAN (O60883) Endothelin B receptor-like protein-2 precurs... 36 0.16
NXF1_MOUSE (Q99JX7) Nuclear RNA export factor 1 (Tip associating... 33 1.0
ST13_YEAST (P33894) Dipeptidyl aminopeptidase A (EC 3.4.14.-) (D... 33 1.3
NXF1_HUMAN (Q9UBU9) Nuclear RNA export factor 1 (Tip associating... 33 1.3
FX42_MOUSE (Q6PDJ6) F-box only protein 42 33 1.3
FX42_HUMAN (Q6P3S6) F-box only protein 42 33 1.3
MT30_YEAST (P39014) MET30 protein 32 2.3
LGR5_HUMAN (O75473) Leucine-rich repeat-containing G protein-cou... 32 2.3
FBW7_MOUSE (Q8VBV4) F-box/WD-repeat protein 7 (F-box and WD-40 d... 32 2.3
FBW7_HUMAN (Q969H0) F-box/WD-repeat protein 7 (F-box and WD-40 d... 32 2.3
TLR3_MOUSE (Q99MB1) Toll-like receptor 3 precursor 32 3.0
POF1_SCHPO (P87053) F-box/WD-repeat protein pof1 (Skp1-binding p... 32 3.0
DTXR_CORDI (P33120) Diphtheria toxin repressor (Iron-dependent d... 32 3.0
XPO1_YEAST (P30822) Exportin 1 (Chromosome region maintenance pr... 31 3.9
POF2_SCHPO (O74783) F-box/LRR-repeat protein 2 (F-box and leucin... 31 3.9
D100_ARATH (Q00874) DNA-damage-repair/toleration protein DRT100 ... 30 6.6
CHAO_DROME (P12024) Chaoptin precursor (Photoreceptor cell-speci... 30 8.7
>ETB2_HUMAN (O60883) Endothelin B receptor-like protein-2 precursor
(ETBR-LP-2) (G protein-coupled receptor 37 like 1)
Length = 481
Score = 35.8 bits (81), Expect = 0.16
Identities = 28/93 (30%), Positives = 46/93 (49%), Gaps = 2/93 (2%)
Query: 155 CLCVPYKVLNSTALVVLKLNALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKILSMS 214
C VP+ ++S + L AL ID H +++ LP ++ + Q KL +I + SM+
Sbjct: 203 CRAVPFMEVSSLGVTTFSLCALGIDRFHVATSTLPKVRPIERCQSILAKLA-VIWVGSMT 261
Query: 215 PLLEDLLLKDL-QVTDNTLAHDDAAALKPFPKL 246
+ +LLL L Q T+ D+ +KP L
Sbjct: 262 LAVPELLLWQLAQEPAPTMGTLDSCIMKPSASL 294
>NXF1_MOUSE (Q99JX7) Nuclear RNA export factor 1 (Tip associating
protein) (Tip-associated protein) (mRNA export factor
TAP)
Length = 618
Score = 33.1 bits (74), Expect = 1.0
Identities = 44/174 (25%), Positives = 74/174 (42%), Gaps = 21/174 (12%)
Query: 141 LQVQHLDVTSSATLCLCVPYKVLNSTALVVLKLNALT-----IDYVHRSSTNLPSLKILH 195
L Q++DV + C+ +++ +L LN + +D + P+LK L+
Sbjct: 237 LVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSSNRLYKLDDMSSIVQKAPNLKTLN 296
Query: 196 LTQVHFLKLKFLIKILSMSPLLEDLLLKDLQVTDNTLAHDD--AAALKPFPKLLRADVSE 253
L+ + L KI + LE+L L + DN +A + FPKLLR D E
Sbjct: 297 LSGNELKTERELDKIKGLK--LEELWLDRNPMCDNFGDQSSYISAIRERFPKLLRLDGHE 354
Query: 254 SSISAFLLPLKLFYNVQFLRSQVLLQTLEQDFSTTQFLNLTHMDLIFHDGYYWI 307
LP + ++V+ + +L + + T+ NL + L F YY I
Sbjct: 355 -------LPPPISFDVE---APTMLPPCKGSYFGTE--NLKSLVLRFLQQYYVI 396
>ST13_YEAST (P33894) Dipeptidyl aminopeptidase A (EC 3.4.14.-) (DPAP
A) (YSCIV)
Length = 931
Score = 32.7 bits (73), Expect = 1.3
Identities = 17/56 (30%), Positives = 26/56 (46%)
Query: 83 EINIDDTSYIHDRDAYDRYYHVIALFLFEMKIHHPIMKTVTILSASPFFNPLPLWL 138
E+ + SY H AY+ Y V LF +++ + K VT + FN P W+
Sbjct: 295 ELGLSKLSYAHFSPAYNYIYFVYENNLFLQQVNSGVAKKVTEDGSKDIFNAKPDWI 350
>NXF1_HUMAN (Q9UBU9) Nuclear RNA export factor 1 (Tip associating
protein) (Tip-associated protein) (mRNA export factor
TAP)
Length = 619
Score = 32.7 bits (73), Expect = 1.3
Identities = 44/174 (25%), Positives = 72/174 (41%), Gaps = 21/174 (12%)
Query: 141 LQVQHLDVTSSATLCLCVPYKVLNSTALVVLKLN-----ALTIDYVHRSSTNLPSLKILH 195
L Q++DV + C+ +++ +L LN +D + P+LKIL+
Sbjct: 238 LVAQNIDVVLNRRSCMAATLRIIEENIPELLSLNLSNNRLYRLDDMSSIVQKAPNLKILN 297
Query: 196 LTQVHFLKLKFLIKILSMSPLLEDLLLKDLQVTDNTLAHDD--AAALKPFPKLLRADVSE 253
L+ + L KI + LE+L L + D +A + FPKLLR D E
Sbjct: 298 LSGNELKSERELDKIKGLK--LEELWLDGNSLCDTFRDQSTYISAIRERFPKLLRLDGHE 355
Query: 254 SSISAFLLPLKLFYNVQFLRSQVLLQTLEQDFSTTQFLNLTHMDLIFHDGYYWI 307
LP + ++V+ + L + + T+ NL + L F YY I
Sbjct: 356 -------LPPPIAFDVE---APTTLPPCKGSYFGTE--NLKSLVLHFLQQYYAI 397
>FX42_MOUSE (Q6PDJ6) F-box only protein 42
Length = 717
Score = 32.7 bits (73), Expect = 1.3
Identities = 18/39 (46%), Positives = 27/39 (69%), Gaps = 1/39 (2%)
Query: 1 MSGLPDELLCEILSFL-PSEEFPSTSLLSKRWRRVWLGM 38
MS LP+E+L ILSFL P +E + +L+ K+W R+ G+
Sbjct: 47 MSELPEEVLEYILSFLSPYQEHKTAALVCKQWYRLIKGV 85
>FX42_HUMAN (Q6P3S6) F-box only protein 42
Length = 717
Score = 32.7 bits (73), Expect = 1.3
Identities = 18/39 (46%), Positives = 27/39 (69%), Gaps = 1/39 (2%)
Query: 1 MSGLPDELLCEILSFL-PSEEFPSTSLLSKRWRRVWLGM 38
MS LP+E+L ILSFL P +E + +L+ K+W R+ G+
Sbjct: 47 MSELPEEVLEYILSFLSPYQEHKTAALVCKQWYRLIKGV 85
>MT30_YEAST (P39014) MET30 protein
Length = 640
Score = 32.0 bits (71), Expect = 2.3
Identities = 20/57 (35%), Positives = 29/57 (50%), Gaps = 4/57 (7%)
Query: 21 FPSTSLLSKRWRRVWLGMPNADRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
FP S +S + M D ISILP EL +ILS L + + + + R+W+ L
Sbjct: 165 FPQLSYISS----LVTHMIKIDFISILPQELSLKILSYLDCQSLCNATRVCRKWQKL 217
>LGR5_HUMAN (O75473) Leucine-rich repeat-containing G
protein-coupled receptor 5 precursor (Orphan G
protein-coupled receptor HG38) (G protein-coupled
receptor 49)
Length = 907
Score = 32.0 bits (71), Expect = 2.3
Identities = 34/145 (23%), Positives = 60/145 (40%), Gaps = 10/145 (6%)
Query: 115 HHPIMKTVTILSASPFFNPLPLWLGCLQVQHLDVTSSATLCLCVPYKVLNSTALVVLKLN 174
H P ++T+T+ AS P G ++ L +T + L P V N N
Sbjct: 303 HLPELRTLTLNGASQI-TEFPDLTGTANLESLTLTGAQISSL--PQTVCNQLP------N 353
Query: 175 ALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKILSMSPLLEDLLLKDLQVTDNTLAH 234
+D + +LPS + Q L+ + +I + + L L+ L + N +A
Sbjct: 354 LQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEI-KVDTFQQLLSLRSLNLAWNKIAI 412
Query: 235 DDAAALKPFPKLLRADVSESSISAF 259
A P L++ D+S + +S+F
Sbjct: 413 IHPNAFSTLPSLIKLDLSSNLLSSF 437
>FBW7_MOUSE (Q8VBV4) F-box/WD-repeat protein 7 (F-box and WD-40
domain protein 7) (F-box protein FBW7) (F-box protein
Fbxw6) (F-box-WD40 repeat protein 6) (SEL-10)
Length = 629
Score = 32.0 bits (71), Expect = 2.3
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 7/61 (11%)
Query: 42 DRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL-------RPRMTEINIDDTSYIHD 94
D IS+LP EL +LS L K ++ + R WR L R + E ID+ +I
Sbjct: 201 DFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKR 260
Query: 95 R 95
R
Sbjct: 261 R 261
>FBW7_HUMAN (Q969H0) F-box/WD-repeat protein 7 (F-box and WD-40
domain protein 7) (F-box protein FBX30) (hCdc4)
(Archipelago homolog) (hAgo) (SEL-10)
Length = 707
Score = 32.0 bits (71), Expect = 2.3
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 7/61 (11%)
Query: 42 DRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL-------RPRMTEINIDDTSYIHD 94
D IS+LP EL +LS L K ++ + R WR L R + E ID+ +I
Sbjct: 279 DFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLLWREKCKEEGIDEPLHIKR 338
Query: 95 R 95
R
Sbjct: 339 R 339
>TLR3_MOUSE (Q99MB1) Toll-like receptor 3 precursor
Length = 905
Score = 31.6 bits (70), Expect = 3.0
Identities = 45/190 (23%), Positives = 81/190 (41%), Gaps = 23/190 (12%)
Query: 134 LPLWLGCLQVQHLDVT--SSATLCLCVPYKVLNSTALVVLKL---------NALTIDYVH 182
LPL L L +QH +++ S T C L+ + + K+ N + +D H
Sbjct: 99 LPL-LKVLNLQHNELSQISDQTFVFCTNLTELDLMSNSIHKIKSNPFKNQKNLIKLDLSH 157
Query: 183 R--SSTNLPS-LKILHLTQVHFLKLKFLIKILSMSPLLEDLLLKDLQVTDNTLAHDDAAA 239
SST L + +++ +L ++ K K L L + L+ L ++ N L
Sbjct: 158 NGLSSTKLGTGVQLENLQELLLAKNKILALRSEELEFLGNSSLRKLDLSSNPLKEFSPGC 217
Query: 240 LKPFPKLLRADVSESSISAFLLPLKLFYNVQ-------FLRSQVLLQTLEQDFSTTQFLN 292
+ KL ++ + ++ L KL + + L + LL T E FS ++ N
Sbjct: 218 FQTIGKLFALLLNNAQLNPHLTE-KLCWELSNTSIQNLSLANNQLLATSESTFSGLKWTN 276
Query: 293 LTHMDLIFHD 302
LT +DL +++
Sbjct: 277 LTQLDLSYNN 286
>POF1_SCHPO (P87053) F-box/WD-repeat protein pof1 (Skp1-binding
protein 1)
Length = 605
Score = 31.6 bits (70), Expect = 3.0
Identities = 14/36 (38%), Positives = 23/36 (63%)
Query: 42 DRISILPDELLCRILSLLPAKQIMVTSLLSRRWRSL 77
D +S+LP E+ RILS L A+ + + +S+ W+ L
Sbjct: 108 DFLSLLPVEISFRILSFLDARSLCQAAQVSKHWKEL 143
>DTXR_CORDI (P33120) Diphtheria toxin repressor (Iron-dependent
diphtheria tox regulatory element) (Tox regulatory
factor)
Length = 226
Score = 31.6 bits (70), Expect = 3.0
Identities = 22/82 (26%), Positives = 43/82 (51%), Gaps = 8/82 (9%)
Query: 54 RILSLLPAKQIMVTSLLSRRWRSLRPRMTEINIDDTSYIHDRDAYDRYYHVIALFLFEMK 113
R L + P + + T+++ R+ R +T+I D + +HD R+ HV++ +
Sbjct: 60 RSLQMTPTGRTLATAVM-RKHRLAERLLTDIIGLDINKVHDEAC--RWEHVMS-----DE 111
Query: 114 IHHPIMKTVTILSASPFFNPLP 135
+ ++K + +S SPF NP+P
Sbjct: 112 VERRLVKVLKDVSRSPFGNPIP 133
>XPO1_YEAST (P30822) Exportin 1 (Chromosome region maintenance
protein 1)
Length = 1084
Score = 31.2 bits (69), Expect = 3.9
Identities = 67/317 (21%), Positives = 120/317 (37%), Gaps = 49/317 (15%)
Query: 11 EILSFL--PSEEFPSTSLLSKRWRRVWLGMPNADRISI------------LPDELLCRIL 56
+IL F P +F + S+L K R W +PN RI I DE+
Sbjct: 53 QILQFSTNPQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMCQDDEVFKTQK 112
Query: 57 SLLPAKQIMVTSLLSRRWRSLRPR-MTEINIDDTSYIHDRDAYDRYYHVIALFLFEMKIH 115
+L+ + + +L + W P + E+ +S ++ + +++ +F+
Sbjct: 113 NLINKSDLTLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLKLLSEEVFDFSAE 172
Query: 116 HPIMKTVTILSASPFFNPLPLWLGCLQVQHLDVTSSATLCLC-----------VPYKVLN 164
L S ++ C QV L+ SS++L + +PY+ +
Sbjct: 173 QMTQAKALHLKNSMSKEFEQIFKLCFQV--LEQGSSSSLIVATLESLLRYLHWIPYRYIY 230
Query: 165 STALVVLKLNALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKILSMSPLLEDLLLKD 224
T + L L+ ++ T +LK LT+V LK+ P DL+ +
Sbjct: 231 ETNI----LELLSTKFMTSPDTRAITLKC--LTEVSNLKI----------PQDNDLIKRQ 274
Query: 225 LQVTDNTLAHDDAAALKPFPKLLRADVSES--SISAFLLPLKLFYNVQFLRSQVLLQTLE 282
+ A ++ P L+A + + + +FL L +F R++ L LE
Sbjct: 275 TVLFFQNTLQQIATSVMPVTADLKATYANANGNDQSFLQDLAMFLTTYLARNRAL---LE 331
Query: 283 QDFSTTQFLNLTHMDLI 299
D S + L H LI
Sbjct: 332 SDESLRELLLNAHQYLI 348
>POF2_SCHPO (O74783) F-box/LRR-repeat protein 2 (F-box and
leucine-rich repeat protein 2) (F-box protein pof2)
Length = 463
Score = 31.2 bits (69), Expect = 3.9
Identities = 42/199 (21%), Positives = 82/199 (41%), Gaps = 24/199 (12%)
Query: 47 LPDELLCRILSLLPAKQIMVTSLLSRRWRS-LRPRMTE---------IN--IDDTSYIHD 94
+P+E+ ILS L A ++ S + WR+ + P + E +N D Y D
Sbjct: 3 VPNEVCFNILSYLEADELRCKSTVCTSWRNFIIPTLWEKVVFQNEAQLNNFFDTLQYSKD 62
Query: 95 RDAYDRYYHVIALFLFEMKIHHPIMKTVTILSASPFFNPLPLWLGCLQVQHLDVTSSATL 154
Y RY + + + +T+ + N GC ++ S L
Sbjct: 63 VSYYFRYLRKLNCSRVRKFLTDKHLMLMTLATGISRLN----LSGCTRI-------SEPL 111
Query: 155 CLCVPYKVLNSTALVVLKLNALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKILSMS 214
+ Y+ LN + + +L + + S N P+LK L++ ++ +++I+
Sbjct: 112 IGKLLYQNLNLVTINFSNIFSLPANILEYISDNCPNLKALNIGNCGLVEDTGMVQIIKRC 171
Query: 215 PLLEDLLLKDL-QVTDNTL 232
P L L++ + ++TD +L
Sbjct: 172 PYLNRLIIPNCRKLTDVSL 190
>D100_ARATH (Q00874) DNA-damage-repair/toleration protein DRT100
precursor
Length = 372
Score = 30.4 bits (67), Expect = 6.6
Identities = 26/75 (34%), Positives = 38/75 (50%), Gaps = 4/75 (5%)
Query: 125 LSASPFFNPLPLWLGCLQVQHLDVTSSATLCLCVPYKVLNSTALVVLKL--NAL--TIDY 180
LS + P+P W+G ++V L +L +P +L+++ L V L NAL TI
Sbjct: 238 LSKNHIEGPIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPD 297
Query: 181 VHRSSTNLPSLKILH 195
V S T L SL + H
Sbjct: 298 VFGSKTYLVSLDLSH 312
>CHAO_DROME (P12024) Chaoptin precursor (Photoreceptor cell-specific
membrane protein)
Length = 1315
Score = 30.0 bits (66), Expect = 8.7
Identities = 29/105 (27%), Positives = 52/105 (48%), Gaps = 10/105 (9%)
Query: 159 PYKVLNSTALVVLKLNALTIDYVHRSSTNLPSLKILHLTQVHFLKLKFLIKILSMSPLLE 218
P + N+ +LKL+ + TNL +L+ ++T++ ++ + ++ S S E
Sbjct: 391 PTETFNAVHYTLLKLD---LSGDRNDPTNLQTLR--NMTRMRNMRSLSISRLGSSSVGPE 445
Query: 219 DLL-----LKDLQVTDNTLAHDDAAALKPFPKLLRADVSESSISA 258
D L+DLQ+T +L+ + A K L R D SE+ IS+
Sbjct: 446 DFKDFGVELEDLQITRASLSGIQSHAFKHVRGLKRLDFSENGISS 490
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.326 0.140 0.428
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,222,441
Number of Sequences: 164201
Number of extensions: 1448252
Number of successful extensions: 4307
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 4295
Number of HSP's gapped (non-prelim): 25
length of query: 339
length of database: 59,974,054
effective HSP length: 111
effective length of query: 228
effective length of database: 41,747,743
effective search space: 9518485404
effective search space used: 9518485404
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 66 (30.0 bits)
Medicago: description of AC146632.7