BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146631.6 - phase: 0 /pseudo
(586 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PPAF_ARATH (Q38924) Iron(III)-zinc(II) purple acid phosphatase p... 117 8e-26
PPAF_PHAVU (P80366) Iron(III)-zinc(II) purple acid phosphatase (... 116 1e-25
PPA_ASPFI (Q12546) Acid phosphatase precursor (EC 3.1.3.2) (pH 6... 87 1e-16
YP77_MYCTU (Q50644) Hypothetical protein Rv2577/MT2654 66 2e-10
PPB_LYSEN (Q05205) Alkaline phosphatase precursor (EC 3.1.3.1) (... 62 4e-09
PPA5_PIG (P09889) Tartrate-resistant acid phosphatase type 5 pre... 38 0.084
LOLA_CAMJE (Q9PNZ0) Outer-membrane lipoprotein carrier protein p... 35 0.54
SES3_HUMAN (P58005) Sestrin 3 34 1.2
RRPL_RINDR (P41357) RNA polymerase beta subunit (EC 2.7.7.48) (L... 32 3.5
DAP2_YEAST (P18962) Dipeptidyl aminopeptidase B (EC 3.4.14.-) (D... 32 4.6
YJHT_ECOLI (P39371) Hypothetical protein yjhT precursor 31 7.9
>PPAF_ARATH (Q38924) Iron(III)-zinc(II) purple acid phosphatase
precursor (EC 3.1.3.2) (PAP)
Length = 469
Score = 117 bits (293), Expect = 8e-26
Identities = 92/316 (29%), Positives = 138/316 (43%), Gaps = 54/316 (17%)
Query: 228 GYIHSALMTGLKPSSAYSYRYGSNSADWSEQTKFSTPPAGGSD-ELKFISFGDMGKT-PL 285
GYIH L+ L+ + Y Y GS WS + F PP G D F GD+G+T
Sbjct: 118 GYIHHCLIDDLEFDTKYYYEIGSGK--WSRRFWFFIPPKSGPDVPYTFGLIGDLGQTYDS 175
Query: 286 DASEEHYIQPGALSVIKAIANEVNSNNVNSVFHIGDISYATGFLAE----WDFFLNLISP 341
+++ HY E+N +V +GD+SYA + WD + +
Sbjct: 176 NSTLSHY--------------EMNPGKGQAVLFVGDLSYADRYPNHDNNRWDTWGRFVER 221
Query: 342 VASRVSYMTAIGNHERDYIDSGSVYVTPDSGG-ECGVPYETYFPMPTAAKDKP---WYSI 397
+ ++ GNHE D++ PD G E P+ + P A WYSI
Sbjct: 222 SVAYQPWIWTAGNHEIDFV--------PDIGEIEPFKPFMNRYHTPHKASGSISPLWYSI 273
Query: 398 EQGSVHFTVISTEHDWSENSEQYNWIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFSSKD 457
++ S + V+S + + QY W++K+L VNR TPWLI + H P Y+S +
Sbjct: 274 KRASAYIIVMSCYSSYGIYTPQYKWLEKELQGVNRTETPWLIVLVHSPFYSSYVHHYMEG 333
Query: 458 QKFINAVEPLLLQNKVDLVLFGHVHNYERT-------CSVYQNKCKAIPIKDQKGVDTYD 510
+ E ++ KVD+V GHVH YER+ ++ C+ PI D+
Sbjct: 334 ETLRVMYEQWFVKYKVDVVFAGHVHAYERSERVSNIAYNIVNGLCE--PISDE------- 384
Query: 511 NRNYSAPVHAVIGMAG 526
SAP++ IG G
Sbjct: 385 ----SAPIYITIGDGG 396
>PPAF_PHAVU (P80366) Iron(III)-zinc(II) purple acid phosphatase (EC
3.1.3.2) (PAP)
Length = 432
Score = 116 bits (291), Expect = 1e-25
Identities = 88/314 (28%), Positives = 136/314 (43%), Gaps = 44/314 (14%)
Query: 225 HDPGYIHSALMTGLKPSSAYSYRYGSNSADWSEQTKFSTPPAGGSD-ELKFISFGDMGKT 283
+ G+IH + LK ++ Y Y G + + + F TPP G D F GD+G++
Sbjct: 82 YSSGFIHHTTIRKLKYNTKYYYEVGLRNT--TRRFSFITPPQTGLDVPYTFGLIGDLGQS 139
Query: 284 -PLDASEEHYIQPGALSVIKAIANEVNSNNVNSVFHIGDISYATGFL----AEWDFFLNL 338
+ + HY E++ +V +GD+SYA + WD +
Sbjct: 140 FDSNTTLSHY--------------ELSPKKGQTVLFVGDLSYADRYPNHDNVRWDTWGRF 185
Query: 339 ISPVASRVSYMTAIGNHERDYIDSGSVYVTPDSGGECGVPYETYFPMPTAAKDKP-WYSI 397
+ ++ GNHE ++ + ++ Y + P + P WYSI
Sbjct: 186 TERSVAYQPWIWTAGNHEIEFAPEIN-----ETEPFKPFSYRYHVPYEASQSTSPFWYSI 240
Query: 398 EQGSVHFTVISTEHDWSENSEQYNWIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFSSKD 457
++ S H V+S+ + + QY W+KK+L V R TPWLI + H P+Y S N +
Sbjct: 241 KRASAHIIVLSSHIAYGRGTPQYTWLKKELRKVKRSETPWLIVLMHSPLYNSYNHHFMEG 300
Query: 458 QKFINAVEPLLLQNKVDLVLFGHVHNYERTCSVYQNKCK-----AIPIKDQKGVDTYDNR 512
+ E ++ KVD+V GHVH YER+ V K P+KDQ
Sbjct: 301 EAMRTKFEAWFVKYKVDVVFAGHVHAYERSERVSNIAYKITDGLCTPVKDQ--------- 351
Query: 513 NYSAPVHAVIGMAG 526
SAPV+ IG AG
Sbjct: 352 --SAPVYITIGDAG 363
>PPA_ASPFI (Q12546) Acid phosphatase precursor (EC 3.1.3.2) (pH
6-optimum acid phosphatase) (APase6)
Length = 614
Score = 87.0 bits (214), Expect = 1e-16
Identities = 102/424 (24%), Positives = 142/424 (33%), Gaps = 144/424 (33%)
Query: 229 YIHSALMTGLKPSSAYSYRY-GSNSADWSEQTKFSTP-PAGGSDELKFISFGDMGKTPLD 286
+ H + GL+P + Y Y+ +N SE F T PAG DMG T
Sbjct: 138 FFHEVSIDGLEPDTTYYYQIPAANGTTQSEVLSFKTSRPAGHPGSFSVAVLNDMGYTNAH 197
Query: 287 ASEEHYIQPGALSVIKAIANEVNSNNVNSVFHIGDISYA----TGFLA------------ 330
+ + ++ A +H GD+SYA +G LA
Sbjct: 198 GTHKQLVKAATEGTAFA-------------WHGGDLSYADDWYSGILACADDWPVCYNGT 244
Query: 331 ----------------------------------------EWDFFLNLISPVASRVSYMT 350
WD + ++ V ++ YM
Sbjct: 245 SSTLPGGGPLPEEYKKPLPAGEIPDQGGPQGGDMSVLYESNWDLWQQWLNNVTLKIPYMV 304
Query: 351 AIGNHE-----------------RDYIDSGSV-------YVTPDSGGECGVPYETYFPMP 386
GNHE D I +G+ Y P S Y+ F MP
Sbjct: 305 LPGNHEASCAEFDGPHNILTAYLNDDIANGTAPTDNLTYYSCPPSQRNF-TAYQHRFRMP 363
Query: 387 ---TAAKDKPWYSIEQGSVHFTVISTEHDWSENSE------------------------- 418
T WYS + G HF I E D++ + E
Sbjct: 364 GPETGGVGNFWYSFDYGLAHFVSIDGETDFANSPEWNFAEDVTGNETLPSESETFITDSG 423
Query: 419 ----------------QYNWIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFSSKDQKFIN 462
Q++W+++DLA V+R TPW+I M HRPMY+S +SS
Sbjct: 424 PFGNVNGSVHETKSYEQWHWLQQDLAKVDRSKTPWVIVMSHRPMYSS--AYSSYQLHVRE 481
Query: 463 AVEPLLLQNKVDLVLFGHVHNYERTCSVYQNKCKAIPIKDQKGVDTYDNRNYSAPVHAVI 522
A E LLL+ VD L GH+H YER + N I +TY N + H +
Sbjct: 482 AFEGLLLKYGVDAYLSGHIHWYERLYPLGAN--GTIDTAAIVNNNTYYAHNGKSITHIIN 539
Query: 523 GMAG 526
GMAG
Sbjct: 540 GMAG 543
>YP77_MYCTU (Q50644) Hypothetical protein Rv2577/MT2654
Length = 529
Score = 66.2 bits (160), Expect = 2e-10
Identities = 82/332 (24%), Positives = 126/332 (37%), Gaps = 61/332 (18%)
Query: 230 IHSALMTGLKPSSAYSYRYGSNSADWSEQTKFSTPPAGGSDELKFISFGDMGKTPLDA-S 288
++ A +T L P + Y Y + T + P G L+F SFGD L +
Sbjct: 126 VNHAHLTNLTPDTDYVYAAVHDGTTPELGTARTAP--SGRKPLRFTSFGDQSTPALGRLA 183
Query: 289 EEHYIQPGALSVIKAIANEVNSNNVNSVFHI--GDISYAT---GFLAEWDFFLNLISPVA 343
+ Y+ S + + +F++ GD+ YA + W + + + A
Sbjct: 184 DGRYVSDNIGSPFAGDIT-IAIERIAPLFNLINGDLCYANLAQDRIRTWSDWFDNNTRSA 242
Query: 344 SRVSYMTAIGNHERDYIDSGSVYVTPDSGGECGVP-YETYFPMPTAAKDKP----WYSIE 398
+M A GNHE + G G Y+TYF +P + WYS
Sbjct: 243 RYRPWMPAAGNHENEV-----------GNGPIGYDAYQTYFAVPDSGSSPQLRGLWYSFT 291
Query: 399 QGSVHFTVISTEHD-------------WSENSEQYNWIKKDLASVNRQ-HTPWLIFMGHR 444
GSV VIS +D EQ W++ +LA+ R W++ H+
Sbjct: 292 AGSVR--VISLHNDDVCYQDGGNSYVRGYSGGEQRRWLQAELANARRDSEIDWVVVCMHQ 349
Query: 445 PMYTSNNGFSSKDQKFINAVEPLLLQNKVDLVLFGHVHNYERTCSVYQNKCKAIPIKDQK 504
++ + + D PL Q +VDLV+ GH H+YER + P++
Sbjct: 350 TAISTADDNNGADLGIRQEWLPLFDQYQVDLVVCGHEHHYER----------SHPLRGAL 399
Query: 505 GVDT-----YDNR-----NYSAPVHAVIGMAG 526
G DT D R + VH VIG G
Sbjct: 400 GTDTRTPIPVDTRSDLIDSTRGTVHLVIGGGG 431
>PPB_LYSEN (Q05205) Alkaline phosphatase precursor (EC 3.1.3.1)
(APASE)
Length = 539
Score = 62.0 bits (149), Expect = 4e-09
Identities = 56/196 (28%), Positives = 78/196 (39%), Gaps = 31/196 (15%)
Query: 310 SNNVNSVFHIGDISYATGFLAE--------WDFFLNLISPVASRVSYMTAIGNHERDYID 361
S N +VF GD +Y +G L+E W F L SP Y T DY +
Sbjct: 176 SINPTAVFTAGDNAYNSGTLSEYNSRYAPTWGRFKALTSPSPGNHDYSTTGAKGYFDYFN 235
Query: 362 SGSVYVTPDSGGECGVPYETYFPMPTAAKDKPWYSIEQGSVHFTVISTEHDWSENSEQYN 421
SG + G P + K +YS + G HF ++T + Q +
Sbjct: 236 G--------SGNQTG---------PAGDRSKGYYSWDVGDWHFVSLNTMSGGTVAQAQID 278
Query: 422 WIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFSSKDQKFINAVEPLLLQNKVDLVLFGHV 481
W+K DLA+ + P H P+ S +S Q + L K DLVL GH
Sbjct: 279 WLKADLAANTK---PCTAAYFHHPL-LSRGSYSGYSQ--VKPFWDALYAAKADLVLVGHD 332
Query: 482 HNYERTCSVYQNKCKA 497
HNY+R + +K A
Sbjct: 333 HNYQRYGKMNPDKAAA 348
>PPA5_PIG (P09889) Tartrate-resistant acid phosphatase type 5
precursor (EC 3.1.3.2) (TR-AP) (Tartrate-resistant acid
ATPase) (TrATPase) (Uteroferrin) (UF)
Length = 340
Score = 37.7 bits (86), Expect = 0.084
Identities = 51/229 (22%), Positives = 88/229 (38%), Gaps = 24/229 (10%)
Query: 272 LKFISFGDMGKTPLDASEEHYIQPGALSVIKAIANEVNSNNVNSVFHIGDISYATGFLAE 331
L+F++ GD G P + ++ KAIA V + + + +GD Y TG
Sbjct: 34 LRFVAVGDWGGVP----NAPFHTAREMANAKAIATTVKTLGADFILSLGDNFYFTGVHDA 89
Query: 332 WD-----FFLNLIS-PVASRVSYMTAIGNHER-DYIDSGSVYVTPDSGGECGVPY-ETYF 383
D F ++ S P V + GNH+ + + Y PY F
Sbjct: 90 KDKRFQETFEDVFSDPSLRNVPWHVLAGNHDHLGNVSAQIAYSKISKRWNFPSPYYRLRF 149
Query: 384 PMPTAAKDKPWYSIEQGSV---HFTVISTEHDWSEN----SEQYNWIKKDLASVNRQHTP 436
+P + + ++ ++ +S + + N Q WIKK LA+ +
Sbjct: 150 KIPRSNVSVAIFMLDTVTLCGNSDDFVSQQPERPRNLALARTQLAWIKKQLAAAKEDY-- 207
Query: 437 WLIFMGHRPMYTSNNGFSSKDQKFINAVEPLLLQNKVDLVLFGHVHNYE 485
++ GH P+++ + + PLL +KV L GH HN +
Sbjct: 208 -VLVAGHYPVWSIAE--HGPTHCLVKQLLPLLTTHKVTAYLCGHDHNLQ 253
>LOLA_CAMJE (Q9PNZ0) Outer-membrane lipoprotein carrier protein
precursor
Length = 169
Score = 35.0 bits (79), Expect = 0.54
Identities = 27/121 (22%), Positives = 54/121 (44%), Gaps = 7/121 (5%)
Query: 420 YNWIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFS----SKDQKFINAVEPLLLQNKVDL 475
+N + + + L + GH + +S SK + +IN + ++++ ++
Sbjct: 22 FNTFSSNFTQIVKSKNSTLSYSGHFILSKDQAYWSYDTPSKKEIYINKNQVTIVEHDLEQ 81
Query: 476 VLFGHVHNYERTCSVYQNKCKAIPIKDQKGVDTYDNRNYSAPVHAVIGMAGFALDKFSNN 535
V+F H+ N +++ KA I K V YDN NY+ ++ + D+F N+
Sbjct: 82 VIFSHLDNIPNLNEIFK---KASLIDKDKLVAKYDNINYTIKLNQEQIQSISYKDEFEND 138
Query: 536 V 536
V
Sbjct: 139 V 139
>SES3_HUMAN (P58005) Sestrin 3
Length = 492
Score = 33.9 bits (76), Expect = 1.2
Identities = 36/150 (24%), Positives = 61/150 (40%), Gaps = 8/150 (5%)
Query: 343 ASRVSYMTAIGNHERDYIDS--GSVYVTPDSGGECGVPYETYFPMPTAAKDKPWYSIEQG 400
+ R+ +T + + Y++S S + G +PY Y + AA+ + Y I
Sbjct: 69 SGRLDNITQVMSLHTQYLESFLRSQFYMLRMDGPLPLPYRHYIAIMAAARHQCSYLINMH 128
Query: 401 SVHFTVISTEHDWSENSEQYNWIKKDLASVNR--QHTPWLIFMGH--RPMYTSNNGFSSK 456
F +W E K+L +N+ H PWLI H + + T N +S
Sbjct: 129 VDEFLKTGGIAEWLNGLEYVPQRLKNLNEINKLLAHRPWLITKEHIQKLVKTGENNWSLP 188
Query: 457 DQKFINAVEPLLLQNKVDLVLFGHVHNYER 486
+ ++AV L + + +FG N ER
Sbjct: 189 E--LVHAVVLLAHYHALASFVFGSGINPER 216
>RRPL_RINDR (P41357) RNA polymerase beta subunit (EC 2.7.7.48) (Large
structural protein) (L protein)
Length = 2183
Score = 32.3 bits (72), Expect = 3.5
Identities = 29/115 (25%), Positives = 49/115 (42%), Gaps = 16/115 (13%)
Query: 233 ALMTGLKPSSAYSYRYGSNSADWSEQTKFSTPPAGGS-DELKFISFGDMGKTPLDASEE- 290
+L + ++ ++ YS+ YG + W+E K + A S +ELK I TP+ S
Sbjct: 1234 SLRSAVRIATVYSWAYGDDDKSWNEALKLAQQRAKVSLEELKMI-------TPISTSTNL 1286
Query: 291 -HYIQPGALSV------IKAIANEVNSNNVNSVFHIGDISYATGFLAEWDFFLNL 338
H ++ + V + +A +N N F I D T F+ + L L
Sbjct: 1287 AHRLRDRSTQVKYSGTSLVRVARYTTISNDNLSFVISDKKVDTNFIYQQGMLLGL 1341
>DAP2_YEAST (P18962) Dipeptidyl aminopeptidase B (EC 3.4.14.-) (DPAP
B) (YSCV)
Length = 818
Score = 32.0 bits (71), Expect = 4.6
Identities = 56/246 (22%), Positives = 89/246 (35%), Gaps = 66/246 (26%)
Query: 292 YIQPGALSVIKAIA---NEVNSNNVNSVFHIGDISYATGFLAEWDFFLNLISPVASRVSY 348
Y P + V+K + NEV ++ +N++ + D TGF + DF R
Sbjct: 596 YGGPNSQQVVKTFSVGFNEVVASQLNAIVVVVD-GRGTGFKGQ-DF----------RSLV 643
Query: 349 MTAIGNHE-RDYIDSGSVY-----VTPDSGGECGVPYETYFPMPTAAKDKPWYSIEQGSV 402
+G++E RD I + S+Y V P G Y Y + T KD G
Sbjct: 644 RDRLGDYEARDQISAASLYGSLTFVDPQKISLFGWSYGGYLTLKTLEKD--------GGR 695
Query: 403 HFTV---ISTEHDWSENSEQYNWIKKDLASVNRQHTPWLIFMGHRPMYTSNNGFSSKDQK 459
HF ++ DW Y R M+T F +
Sbjct: 696 HFKYGMSVAPVTDWRFYDSVYT---------------------ERYMHTPQENFDGYVES 734
Query: 460 FINAVEPLLLQNKVDLVLFG----HVHNYERTCSVYQNKCKAIPIKDQKGVDTYDNRNYS 515
++ V L N+ L++ G +VH +QN K + + D GV+ YD +
Sbjct: 735 SVHNVTALAQANRF-LLMHGTGDDNVH--------FQNSLKFLDLLDLNGVENYDVHVFP 785
Query: 516 APVHAV 521
H++
Sbjct: 786 DSDHSI 791
>YJHT_ECOLI (P39371) Hypothetical protein yjhT precursor
Length = 368
Score = 31.2 bits (69), Expect = 7.9
Identities = 25/97 (25%), Positives = 39/97 (39%), Gaps = 16/97 (16%)
Query: 360 IDSGSVYVTPDSGGECGVPYETYFPMPTAAKDKPWYSIEQ--GSVHFTVISTEHDWS--- 414
ID+ +VY+ S G ++ + T AKDK W ++ G S D +
Sbjct: 36 IDNDTVYIGLGSAGTA------WYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYV 89
Query: 415 -----ENSEQYNWIKKDLASVNRQHTPWLIFMGHRPM 446
+NSE + D+ N + W+ M H PM
Sbjct: 90 FGGIGKNSEGLTQVFNDVHKYNPKTNSWVKLMSHAPM 126
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.325 0.138 0.444
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,025,216
Number of Sequences: 164201
Number of extensions: 3064314
Number of successful extensions: 8127
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 8106
Number of HSP's gapped (non-prelim): 15
length of query: 586
length of database: 59,974,054
effective HSP length: 116
effective length of query: 470
effective length of database: 40,926,738
effective search space: 19235566860
effective search space used: 19235566860
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 69 (31.2 bits)
Medicago: description of AC146631.6