BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146587.12 - phase: 0 /pseudo
(215 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
APG_BRANA (P40603) Anter-specific proline-rich protein APG (Prot... 78 1e-14
APG_ARATH (P40602) Anter-specific proline-rich protein APG precu... 74 3e-13
EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early nodule... 43 5e-04
SSK2_YEAST (P53599) MAP kinase kinase kinase SSK2 (EC 2.7.1.-) (... 33 0.50
CRTC_MAIZE (Q9SP22) Calreticulin precursor 31 2.5
PHT2_PSEPU (Q05182) Phthalate 4,5-dioxygenase oxygenase reductas... 30 3.2
TDH_DEIRA (Q9RTU4) L-threonine 3-dehydrogenase (EC 1.1.1.103) 30 4.2
MNN9_YEAST (P39107) Mannan polymerase complexes MNN9 subunit (M-... 30 5.5
POP4_YEAST (P38336) RNases MRP/P 32.9 kDa subunit (RNA processin... 29 7.2
GP10_RAT (Q64121) Prolactin-releasing peptide receptor (PrRP rec... 29 7.2
GLYC_SHEEP (P35623) Serine hydroxymethyltransferase, cytosolic (... 29 7.2
GLND_NEIMB (Q9JZB4) [Protein-PII] uridylyltransferase (EC 2.7.7.... 29 7.2
GLND_NEIMA (Q9JUC9) [Protein-PII] uridylyltransferase (EC 2.7.7.... 29 7.2
RNZ_STRMU (Q8DT90) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA... 29 9.4
MINC_GLOVI (Q7NJ40) Probable septum site-determining protein minC 29 9.4
>APG_BRANA (P40603) Anter-specific proline-rich protein APG (Protein
CEX) (Fragment)
Length = 449
Score = 78.2 bits (191), Expect = 1e-14
Identities = 42/160 (26%), Positives = 81/160 (50%), Gaps = 4/160 (2%)
Query: 1 MYRQLDYFEEYQHRVASMIGAARAEKLVNKALVLITVGGNDFVNNYYLVPYSARSREYSL 60
M QL YF++Y+ R+ ++G + +K+V+K ++ G ND + Y+ A+ + +
Sbjct: 231 MLDQLTYFQDYKKRMKKLVGKKKTKKIVSKGAAIVVAGSNDLIYTYF--GNGAQHLKNDV 288
Query: 61 QDYVKFLIIEYRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRGTDGGCSAELQRAATL 120
+ + + +LY GARR+ V GT P+GC P++ + C+ +L AA L
Sbjct: 289 DSFTTMMADSAASFVLQLYGYGARRIGVIGTPPIGCTPSQRVKKKKI--CNEDLNYAAQL 346
Query: 121 YNPQLQHMVQGLNKKIGKDVFIATNTALIHSDFVTNPKAY 160
+N +L ++ L+K + + + I S + +P+ Y
Sbjct: 347 FNSKLVIILGQLSKTLPNSTIVYGDIYSIFSKMLESPEDY 386
>APG_ARATH (P40602) Anter-specific proline-rich protein APG
precursor
Length = 534
Score = 73.6 bits (179), Expect = 3e-13
Identities = 46/170 (27%), Positives = 81/170 (47%), Gaps = 14/170 (8%)
Query: 1 MYRQLDYFEEYQHRVASMI----------GAARAEKLVNKALVLITVGGNDFVNNYYLVP 50
M QL YF++Y +V ++ G + +L++K + ++ G ND + Y+
Sbjct: 304 MLDQLTYFQDYIEKVNRLVRQEKSQYKLAGLEKTNQLISKGVAIVVGGSNDLIITYF--G 361
Query: 51 YSARSREYSLQDYVKFLIIEYRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRGTDGGC 110
A+ + + Y + + +LY GARR+ V GT PLGCVP++ + C
Sbjct: 362 SGAQRLKNDIDSYTTIIADSAASFVLQLYGYGARRIGVIGTPPLGCVPSQRLKKKKI--C 419
Query: 111 SAELQRAATLYNPQLQHMVQGLNKKIGKDVFIATNTALIHSDFVTNPKAY 160
+ EL A+ L+N +L ++ L+K + F+ + I S + P AY
Sbjct: 420 NEELNYASQLFNSKLLLILGQLSKTLPNSTFVYMDIYTIISQMLETPAAY 469
>EST_HEVBR (Q7Y1X1) Esterase precursor (EC 3.1.1.-) (Early
nodule-specific protein homolog) (Latex allergen Hev b
13)
Length = 391
Score = 43.1 bits (100), Expect = 5e-04
Identities = 29/140 (20%), Positives = 56/140 (39%), Gaps = 10/140 (7%)
Query: 25 EKLVNKALVLITVGGNDFVNNYYLVPYSARSREYSLQDYVKFLIIEYRKLLERLYDIGAR 84
E KAL +G ND + + + V L+ + ++++YD+GAR
Sbjct: 163 EYYFEKALYTFDIGQNDLTEGFLNLTVE------EVNATVPDLVNSFSANVKKIYDLGAR 216
Query: 85 RVLVTGTGPLGCVPAEMA----MRGTDGGCSAELQRAATLYNPQLQHMVQGLNKKIGKDV 140
+ TGP+GC+ + GC+ A +N +L+ +V L K +
Sbjct: 217 TFWIHNTGPIGCLSFILTYFPWAEKDSAGCAKAYNEVAQHFNHKLKEIVAQLRKDLPLAT 276
Query: 141 FIATNTALIHSDFVTNPKAY 160
F+ + + + P+ +
Sbjct: 277 FVHVDIYSVKYSLFSEPEKH 296
>SSK2_YEAST (P53599) MAP kinase kinase kinase SSK2 (EC 2.7.1.-)
(Suppressor of sensor kinase 2)
Length = 1579
Score = 33.1 bits (74), Expect = 0.50
Identities = 38/167 (22%), Positives = 73/167 (42%), Gaps = 22/167 (13%)
Query: 59 SLQDYVKFLIIEYRKLLERLYDIGARRV------------LVTGTGPLGCVPAEMA--MR 104
+ Q++V++ I ++ LL++L + G + L+ LGC ++ ++
Sbjct: 813 AFQNFVRYKIEDHNYLLKQLKETGHFLIYTGGYLEQNGTYLIGSPELLGCKDDDILRIIK 872
Query: 105 GTDGGCSA----ELQRAATLYNPQLQHMVQGLNKKIGKDVFI-ATNTALIHSDFVTNPKA 159
+D GC E+ + T+YN + N +G D+ T I +D T P++
Sbjct: 873 NSDIGCDLVPKLEINNSLTIYNALDDNW--NSNSSLGSDISNDGTPFYYIKNDLTTQPRS 930
Query: 160 YELLVVDKEPTTGLVCAHHSLIYAQIETCMHFGMHSIHLKRQTKLLW 206
Y V++EP + Y ++ET ++ + + L Q LLW
Sbjct: 931 YNGNRVNREPDFENSRSTEEEFY-ELETRLNSLGYVLVLTPQEPLLW 976
>CRTC_MAIZE (Q9SP22) Calreticulin precursor
Length = 420
Score = 30.8 bits (68), Expect = 2.5
Identities = 34/141 (24%), Positives = 58/141 (41%), Gaps = 12/141 (8%)
Query: 45 NYYLVPYSARSREYSLQDYVKFLIIEYRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMR 104
+Y SA E+S +D K L++++ E+ D G V + G +
Sbjct: 77 DYRFYAISAEYPEFSNKD--KTLVLQFSVKHEQKLDCGGGYVKLLGGD------VDQKTL 128
Query: 105 GTDGGCSAELQRAATLYNPQLQHMV---QGLNKKIGKDVFIATNTALIHSDFVTNPKA-Y 160
G D S + + Y+ + H + G N I KDV T+ F+ P A Y
Sbjct: 129 GGDTSYSIISRPDISRYSTKKVHTILTKDGKNHLIKKDVPCQTDQLTHVYTFIIRPDATY 188
Query: 161 ELLVVDKEPTTGLVCAHHSLI 181
+L+ ++E TG + H ++
Sbjct: 189 SILIDNEEKHTGSIYEHWDIL 209
>PHT2_PSEPU (Q05182) Phthalate 4,5-dioxygenase oxygenase reductase
subunit (EC 1.14.12.7)
Length = 324
Score = 30.4 bits (67), Expect = 3.2
Identities = 28/118 (23%), Positives = 45/118 (37%), Gaps = 9/118 (7%)
Query: 85 RVLVTGTGPLGCVPAEMAMRGTDGGCSAELQRAATLYNPQLQHMVQGLNKKIGKDVFIAT 144
R + G +G P MR + NP+L L +G +
Sbjct: 115 RQFIFVAGGIGITPILSMMRHLKASTDLPFKLYYCTRNPELTAFRDEL---LGAEF---A 168
Query: 145 NTALIHSDFVTNPKAYELLVVDKEPTTG---LVCAHHSLIYAQIETCMHFGMHSIHLK 199
NT +IH DF AY+ V +P++G C L+ + ++ H+ SIH +
Sbjct: 169 NTVVIHHDFGNRADAYDFWPVFDKPSSGTHVYCCGPRPLMDSVLDMTGHWPPGSIHFE 226
>TDH_DEIRA (Q9RTU4) L-threonine 3-dehydrogenase (EC 1.1.1.103)
Length = 348
Score = 30.0 bits (66), Expect = 4.2
Identities = 11/24 (45%), Positives = 17/24 (70%)
Query: 79 YDIGARRVLVTGTGPLGCVPAEMA 102
+D+ VL+TG GP+GC+ A +A
Sbjct: 160 FDLVGEDVLITGAGPIGCMAAAVA 183
>MNN9_YEAST (P39107) Mannan polymerase complexes MNN9 subunit (M-pol
I subunit MNN9) (M-Pol II subunit MNN9)
Length = 394
Score = 29.6 bits (65), Expect = 5.5
Identities = 25/92 (27%), Positives = 38/92 (41%), Gaps = 21/92 (22%)
Query: 68 IIEYRKLLERLYDIGARRVLVTGTGPLGCVPAEMAMRGTDGGCS---AELQRAATLY-NP 123
I YR L+ YD G EMA+ G GGC+ AE+ R ++ N
Sbjct: 309 IATYRPLMAHFYDAN------------GVPGEEMALDGVGGGCTLVKAEVHRDGAMFPNF 356
Query: 124 QLQHMVQ-----GLNKKIGKDVFIATNTALIH 150
H+++ + K++ DVF N + H
Sbjct: 357 PFYHLIETEGFAKMAKRLNYDVFGLPNYLVYH 388
>POP4_YEAST (P38336) RNases MRP/P 32.9 kDa subunit (RNA processing
protein POP4)
Length = 279
Score = 29.3 bits (64), Expect = 7.2
Identities = 26/102 (25%), Positives = 43/102 (41%), Gaps = 16/102 (15%)
Query: 106 TDGGCSAELQRAA-----TLYNPQLQHMVQGLNKKIGKDVF--IATNTALIHSDFVTNPK 158
TDGG ++ LQR L N Q ++ +K++ K + I N+ + +++ N K
Sbjct: 38 TDGGLTSRLQRQQRKSKLNLDNLQKVSQLESADKQLEKRDYQRINKNSKIALREYINNCK 97
Query: 159 ---------AYELLVVDKEPTTGLVCAHHSLIYAQIETCMHF 191
AYE + DKE + H IY + + F
Sbjct: 98 KNTKKCLKLAYENKITDKEDLLHYIEEKHPTIYESLPQYVDF 139
>GP10_RAT (Q64121) Prolactin-releasing peptide receptor (PrRP
receptor) (PrRPR) (G protein-coupled receptor GPR10)
(UHR-1)
Length = 370
Score = 29.3 bits (64), Expect = 7.2
Identities = 16/49 (32%), Positives = 25/49 (50%), Gaps = 3/49 (6%)
Query: 87 LVTGTGPLGCVPAEMAMRGTDGGCSAELQRAATL---YNPQLQHMVQGL 132
L +G P G PA + ++ SA + RAA + + QL H ++GL
Sbjct: 14 LFSGPSPAGSTPANQSAEASESNVSATVPRAAAVTPFQSLQLVHQLKGL 62
>GLYC_SHEEP (P35623) Serine hydroxymethyltransferase, cytosolic (EC
2.1.2.1) (Serine methylase) (Glycine
hydroxymethyltransferase) (SHMT)
Length = 483
Score = 29.3 bits (64), Expect = 7.2
Identities = 25/98 (25%), Positives = 46/98 (46%), Gaps = 6/98 (6%)
Query: 25 EKLVNKALVLITVGG--NDFVNNYYLVPYSARSREYSLQDYVKFLIIEYRKLLERLYDIG 82
E L+N A+ GG N + + A + E+ + Y + ++ R L E L +G
Sbjct: 288 ESLINSAVFPGLQGGPHNHAIAGVAVALKQAMTPEF--RAYQRQVVANCRALAEALMGLG 345
Query: 83 ARRVLVTGTGPLGCVPAEMAMRGTDGGCSAELQRAATL 120
R +VTG + ++ +GTDGG + ++ A ++
Sbjct: 346 YR--VVTGGSDNHLILVDLRSKGTDGGRAEKVLEACSI 381
>GLND_NEIMB (Q9JZB4) [Protein-PII] uridylyltransferase (EC
2.7.7.59) (PII uridylyl-transferase) (Uridylyl removing
enzyme) (UTase)
Length = 852
Score = 29.3 bits (64), Expect = 7.2
Identities = 14/33 (42%), Positives = 19/33 (57%)
Query: 7 YFEEYQHRVASMIGAARAEKLVNKALVLITVGG 39
+F EY V +++ A AE N AL L+ VGG
Sbjct: 32 FFREYTAAVETLLAALWAEYFQNSALCLMAVGG 64
>GLND_NEIMA (Q9JUC9) [Protein-PII] uridylyltransferase (EC
2.7.7.59) (PII uridylyl-transferase) (Uridylyl removing
enzyme) (UTase)
Length = 852
Score = 29.3 bits (64), Expect = 7.2
Identities = 14/33 (42%), Positives = 19/33 (57%)
Query: 7 YFEEYQHRVASMIGAARAEKLVNKALVLITVGG 39
+F EY V +++ A AE N AL L+ VGG
Sbjct: 32 FFREYTAAVETLLAALWAEYFQNSALCLMAVGG 64
>RNZ_STRMU (Q8DT90) Ribonuclease Z (EC 3.1.26.11) (RNase Z) (tRNA 3
endonuclease)
Length = 309
Score = 28.9 bits (63), Expect = 9.4
Identities = 18/71 (25%), Positives = 34/71 (47%), Gaps = 7/71 (9%)
Query: 96 CVPAEMAMRGTDGGCSAELQRAATL-YNPQLQHMVQGLNKKIGKDVFIATNTALIHSDFV 154
CV + + +G AE +AA L + P + K G+DV + T +I D++
Sbjct: 149 CVGYRVVQKDLEGTLDAEKLKAAGLPFGPLFGRV------KNGQDVVLEDGTTIIAKDYI 202
Query: 155 TNPKAYELLVV 165
+ PK +++ +
Sbjct: 203 SAPKKGKVITI 213
>MINC_GLOVI (Q7NJ40) Probable septum site-determining protein minC
Length = 278
Score = 28.9 bits (63), Expect = 9.4
Identities = 22/98 (22%), Positives = 38/98 (38%), Gaps = 7/98 (7%)
Query: 97 VPAEMAMRGTDGGCSAELQRAATLYNPQLQHMVQGLNKKIGKDVFIATNTALIHSDFVTN 156
V + A+ G S E Q A NP ++GL + + + + +L+H V
Sbjct: 130 VRRQTAVAAAVAGLSVEQQEAPPEENPDKPPALRGLAEPLYLQTTLRSGMSLVHPGTV-- 187
Query: 157 PKAYELLVVDKEPTTGLVCAHHSLIYAQIETCMHFGMH 194
++V D P +V L++ + H G H
Sbjct: 188 -----IVVGDVNPGAEIVADGDILVWGTLRGVAHAGAH 220
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.138 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,886,394
Number of Sequences: 164201
Number of extensions: 999472
Number of successful extensions: 2243
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 2236
Number of HSP's gapped (non-prelim): 15
length of query: 215
length of database: 59,974,054
effective HSP length: 106
effective length of query: 109
effective length of database: 42,568,748
effective search space: 4639993532
effective search space used: 4639993532
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146587.12