BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146587.10 + phase: 0 /pseudo
(1157 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LYTR_STRA5 (Q8E217) Sensory transduction protein lytR 37 0.31
LYTR_STRA3 (Q8E7H3) Sensory transduction protein lytR 37 0.31
MYHB_CHICK (P10587) Myosin heavy chain, gizzard smooth muscle 37 0.40
MYHB_RABIT (P35748) Myosin heavy chain, smooth muscle isoform (S... 36 0.53
MYHB_MOUSE (O08638) Myosin heavy chain, smooth muscle isoform (S... 36 0.53
MYHB_HUMAN (P35749) Myosin heavy chain, smooth muscle isoform (S... 36 0.53
DMD_MOUSE (P11531) Dystrophin 34 2.0
F49A_MOUSE (Q8BHZ0) Protein FAM49A 32 7.6
F49A_HUMAN (Q9H0Q0) Protein FAM49A 32 7.6
YCO2_ARATH (Q9LME2) Hypothetical protein At1g22260 32 10.0
TRPC_BUCAP (P42393) Tryptophan biosynthesis protein trpCF [Inclu... 32 10.0
SPOF_SCHPO (Q10411) Sporulation-specific protein 15 32 10.0
CNRB_CANFA (P33726) Rod cGMP-specific 3',5'-cyclic phosphodieste... 32 10.0
CNRB_BOVIN (P23439) Rod cGMP-specific 3',5'-cyclic phosphodieste... 32 10.0
>LYTR_STRA5 (Q8E217) Sensory transduction protein lytR
Length = 246
Score = 37.0 bits (84), Expect = 0.31
Identities = 28/113 (24%), Positives = 54/113 (47%), Gaps = 4/113 (3%)
Query: 241 LSTVEDILQVLIVFAVESLELDFALLFPERHILLRVLPVLVVLVTSSEKDSESLYKRVKI 300
L+T D+ Q + A S+EL L +R +++++ ++ V E ++ K I
Sbjct: 123 LTTASDVEQAIPKKA--SVEL-LTLTLSDRSVVVKMQDIVAASVEDGELTVSTVQKTYTI 179
Query: 301 NRLINIFKNEAVIPAFPDLHLSPAAIMKELSTYFPKFSSQTRLLTLAAPHELP 353
+ +N FK+ AV P F +H + ++ + P F + T LL ++ + P
Sbjct: 180 RKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWF-NHTLLLIMSNGEKFP 231
>LYTR_STRA3 (Q8E7H3) Sensory transduction protein lytR
Length = 246
Score = 37.0 bits (84), Expect = 0.31
Identities = 28/113 (24%), Positives = 54/113 (47%), Gaps = 4/113 (3%)
Query: 241 LSTVEDILQVLIVFAVESLELDFALLFPERHILLRVLPVLVVLVTSSEKDSESLYKRVKI 300
L+T D+ Q + A S+EL L +R +++++ ++ V E ++ K I
Sbjct: 123 LTTASDVEQAIPKKA--SVEL-LTLTLSDRSVVVKMQDIVAASVEDGELTVSTVQKTYTI 179
Query: 301 NRLINIFKNEAVIPAFPDLHLSPAAIMKELSTYFPKFSSQTRLLTLAAPHELP 353
+ +N FK+ AV P F +H + ++ + P F + T LL ++ + P
Sbjct: 180 RKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWF-NHTLLLIMSNGEKFP 231
>MYHB_CHICK (P10587) Myosin heavy chain, gizzard smooth muscle
Length = 1978
Score = 36.6 bits (83), Expect = 0.40
Identities = 40/187 (21%), Positives = 81/187 (42%), Gaps = 15/187 (8%)
Query: 43 EYCDVAAYRLSLSEDTKALN----QLSSLTQEGKEMASVLYTYRSCVKALPQLPDSMKQS 98
+Y D R L+E L ++SL E + L K + L ++ +
Sbjct: 1272 KYSDGERVRTELNEKVHKLQIEVENVTSLLNEAESKNIKL------TKDVATLGSQLQDT 1325
Query: 99 QADLYLETYQVLDLEMSRLREIQRWQASASSKLATDMQRFSRPERRINGPTISHLWSMLK 158
Q L ET Q L++ ++LR+++ + S +L +++ ER I+ TI S K
Sbjct: 1326 QELLQEETRQKLNVT-TKLRQLEDDKNSLQEQLDEEVEAKQNLERHISTLTIQLSDSKKK 1384
Query: 159 LFDVLVQLDHLKNAKASIPNDFSWYKRTFTQVSGQWQDTDS----MREELDDLQIFLSTR 214
L + ++ ++ K + + + F + + + + +++ELDDL + L +
Sbjct: 1385 LQEFTATVETMEEGKKKLQREIESLTQQFEEKAASYDKLEKTKNRLQQELDDLVVDLDNQ 1444
Query: 215 WAILLNL 221
++ NL
Sbjct: 1445 RQLVSNL 1451
>MYHB_RABIT (P35748) Myosin heavy chain, smooth muscle isoform (SMMHC)
Length = 1972
Score = 36.2 bits (82), Expect = 0.53
Identities = 31/140 (22%), Positives = 64/140 (45%), Gaps = 5/140 (3%)
Query: 86 KALPQLPDSMKQSQADLYLETYQVLDLEMSRLREIQRWQASASSKLATDMQRFSRPERRI 145
K + L ++ +Q L ET Q L++ ++LR+++ + S +L +M+ ER I
Sbjct: 1308 KEVASLGSQLQDTQELLQEETRQKLNVS-TKLRQLEDERNSLQEQLDEEMEAKQNLERHI 1366
Query: 146 NGPTISHLWSMLKLFDVLVQLDHLKNAKASIPNDFSWYKRTFTQVSGQWQDTDS----MR 201
+ I S KL D ++ L+ K + + + + + + + ++
Sbjct: 1367 STLNIQLSDSKKKLQDFASTVESLEEGKKRFQKEIESLTQQYEEKAAAYDKLEKTKNRLQ 1426
Query: 202 EELDDLQIFLSTRWAILLNL 221
+ELDDL + L + ++ NL
Sbjct: 1427 QELDDLVVDLDNQRQLVSNL 1446
>MYHB_MOUSE (O08638) Myosin heavy chain, smooth muscle isoform (SMMHC)
Length = 1972
Score = 36.2 bits (82), Expect = 0.53
Identities = 39/181 (21%), Positives = 80/181 (43%), Gaps = 19/181 (10%)
Query: 51 RLSLSEDTKAL-NQLSSLT-----QEGKEMASVLYTYRSCVKALPQLPDSMKQSQADLYL 104
R LS+ L N++ S+T EGK + K + L ++ +Q L
Sbjct: 1275 RAELSDKVHKLQNEVESVTGMLNEAEGKAI--------KLAKDVASLGSQLQDTQELLQE 1326
Query: 105 ETYQVLDLEMSRLREIQRWQASASSKLATDMQRFSRPERRINGPTISHLWSMLKLFDVLV 164
ET Q L++ ++LR+++ + S +L +M+ ER ++ I S KL D
Sbjct: 1327 ETRQKLNVS-TKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDFAS 1385
Query: 165 QLDHLKNAKASIPNDFSWYKRTFTQVSGQWQDTDS----MREELDDLQIFLSTRWAILLN 220
++ ++ K + + + + + + + + +++ELDDL + L + ++ N
Sbjct: 1386 TIEVMEEGKKRLQKEMEGLSQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSN 1445
Query: 221 L 221
L
Sbjct: 1446 L 1446
>MYHB_HUMAN (P35749) Myosin heavy chain, smooth muscle isoform (SMMHC)
Length = 1972
Score = 36.2 bits (82), Expect = 0.53
Identities = 31/140 (22%), Positives = 64/140 (45%), Gaps = 5/140 (3%)
Query: 86 KALPQLPDSMKQSQADLYLETYQVLDLEMSRLREIQRWQASASSKLATDMQRFSRPERRI 145
K + L ++ +Q L ET Q L++ ++LR+++ + S +L +M+ ER I
Sbjct: 1308 KDVASLSSQLQDTQELLQEETRQKLNVS-TKLRQLEEERNSLQDQLDEEMEAKQNLERHI 1366
Query: 146 NGPTISHLWSMLKLFDVLVQLDHLKNAKASIPNDFSWYKRTFTQVSGQWQDTDS----MR 201
+ I S KL D ++ L+ K + + + + + + + ++
Sbjct: 1367 STLNIQLSDSKKKLQDFASTVEALEEGKKRFQKEIENLTQQYEEKAAAYDKLEKTKNRLQ 1426
Query: 202 EELDDLQIFLSTRWAILLNL 221
+ELDDL + L + ++ NL
Sbjct: 1427 QELDDLVVDLDNQRQLVSNL 1446
>DMD_MOUSE (P11531) Dystrophin
Length = 3678
Score = 34.3 bits (77), Expect = 2.0
Identities = 43/181 (23%), Positives = 76/181 (41%), Gaps = 34/181 (18%)
Query: 140 RPERRINGPTISHLWSMLK--LFDVLVQLDHLKNAKASIPN--DFSWYKRTFTQVSGQWQ 195
R +RR ISH W K D+L LD ++ AS+P D K ++ + +
Sbjct: 1867 RSQRRKKALEISHQWYQYKRQADDLLKCLDEIEKKLASLPEPRDERKLKEIDRELQKKKE 1926
Query: 196 DTDSMREELDDL------------QIFLSTRWAILLNLHVEMFRVNKYPVYLLHIFSLST 243
+ +++R + + L QI LS RW + + + R+N ++ LH
Sbjct: 1927 ELNAVRRQAEGLSENGAAMAVEPTQIQLSKRWRQIESNFAQFRRLNFAQIHTLH------ 1980
Query: 244 VEDILQVLIVFAVESLELDFALL----FPERHILLRVLPVLVVLVTSSE---KDSESLYK 296
+ +V E + LD + + E +L+ L + L+ + E KD E L+K
Sbjct: 1981 -----EETMVVTTEDMPLDVSYVPSTYLTEISHILQALSEVDHLLNTPELCAKDFEDLFK 2035
Query: 297 R 297
+
Sbjct: 2036 Q 2036
>F49A_MOUSE (Q8BHZ0) Protein FAM49A
Length = 323
Score = 32.3 bits (72), Expect = 7.6
Identities = 31/137 (22%), Positives = 64/137 (46%), Gaps = 17/137 (12%)
Query: 62 NQLSSLTQEGKEMASVLYTYRSCVKALPQLPDSMKQSQADLYLE--TYQVLDLEMSRLRE 119
NQ+S++ Q+ + + + L Y+ A P++ D++ Q+ D+ L+ + + + RL+
Sbjct: 36 NQISAVLQDSESILTDLQAYKG---AGPEIRDAI-QNPNDIQLQEKAWNAVCPLVVRLKR 91
Query: 120 IQRWQASASSKLATDMQRFSRPERRINGPTISHLW-------SMLKLFDVLVQLDHLKNA 172
+ L + ++ + P PT HL ++ ++ D LK
Sbjct: 92 FYEFSIRLEKALQSLLESLTCPPYT---PT-QHLEREQALAKEFAEILHFTLRFDELKMR 147
Query: 173 KASIPNDFSWYKRTFTQ 189
+I NDFS+Y+RT ++
Sbjct: 148 NPAIQNDFSYYRRTISR 164
>F49A_HUMAN (Q9H0Q0) Protein FAM49A
Length = 323
Score = 32.3 bits (72), Expect = 7.6
Identities = 31/137 (22%), Positives = 64/137 (46%), Gaps = 17/137 (12%)
Query: 62 NQLSSLTQEGKEMASVLYTYRSCVKALPQLPDSMKQSQADLYLE--TYQVLDLEMSRLRE 119
NQ+S++ Q+ + + + L Y+ A P++ D++ Q+ D+ L+ + + + RL+
Sbjct: 36 NQISAVLQDSESILADLQAYKG---AGPEIRDAI-QNPNDIQLQEKAWNAVCPLVVRLKR 91
Query: 120 IQRWQASASSKLATDMQRFSRPERRINGPTISHLW-------SMLKLFDVLVQLDHLKNA 172
+ L + ++ + P PT HL ++ ++ D LK
Sbjct: 92 FYEFSIRLEKALQSLLESLTCPPYT---PT-QHLEREQALAKEFAEILHFTLRFDELKMR 147
Query: 173 KASIPNDFSWYKRTFTQ 189
+I NDFS+Y+RT ++
Sbjct: 148 NPAIQNDFSYYRRTISR 164
>YCO2_ARATH (Q9LME2) Hypothetical protein At1g22260
Length = 871
Score = 32.0 bits (71), Expect = 10.0
Identities = 34/148 (22%), Positives = 65/148 (42%), Gaps = 7/148 (4%)
Query: 17 LEDEQPEVQGPGVWVSTERGATESPIEYCDVAAYRLSLSEDTKALNQLSSLTQEGKEMAS 76
LE+ + E Q ++ TER T S IE D +L S + LN + +L + +++
Sbjct: 181 LEELKLEKQQKEMFYQTERCGTASLIEKKDAVITKLEASAAERKLN-IENLNSQLEKVHL 239
Query: 77 VLYTYRSCVKALPQLPDSMK------QSQADLYLETYQVLDLEMSRLREIQRWQASASSK 130
L T VK L + + ++ Q AD E + E+ +L E+ ++ + ++
Sbjct: 240 ELTTKEDEVKDLVSIQEKLEKEKTSVQLSADNCFEKLVSSEQEVKKLDELVQYLVAELTE 299
Query: 131 LATDMQRFSRPERRINGPTISHLWSMLK 158
L F +++G +H+ + K
Sbjct: 300 LDKKNLTFKEKFDKLSGLYDTHIMLLQK 327
>TRPC_BUCAP (P42393) Tryptophan biosynthesis protein trpCF
[Includes: Indole-3-glycerol phosphate synthase (EC
4.1.1.48) (IGPS); N-(5'-phospho-ribosyl)anthranilate
isomerase (EC 5.3.1.24) (PRAI)]
Length = 451
Score = 32.0 bits (71), Expect = 10.0
Identities = 34/155 (21%), Positives = 71/155 (44%), Gaps = 30/155 (19%)
Query: 637 PWMLVDCVLESPNSGLLE---SVLMPFDIYNDSAQQALVLLKQRFLYDEIE--------- 684
P+ +++C SP+ G++ +++ +IY + A VL +++ + IE
Sbjct: 49 PFFILECKKSSPSLGIIRKKFNLIEIANIYKNYASAISVLTDEKYFHGNIEYINVVRRCV 108
Query: 685 AEVDHCFDIFVARLCETIFTYYKSWA----ASELLDPTFLFASENAEKYAVQPMRLNMLL 740
++ C D F+ + YY + A S L D +L S A++ + + +L
Sbjct: 109 SQPILCKDFFIDSYQVYLARYYSADAILLMLSVLNDSQYLELSRIAKE-----LNMGVLT 163
Query: 741 KMTRVKELEKLLDVLKHSHELLSI------DLSVD 769
++ +KEL++ +K + ++ I DLS+D
Sbjct: 164 EINNIKELKR---AIKLNASVIGINNRNLHDLSID 195
>SPOF_SCHPO (Q10411) Sporulation-specific protein 15
Length = 1957
Score = 32.0 bits (71), Expect = 10.0
Identities = 47/177 (26%), Positives = 75/177 (41%), Gaps = 20/177 (11%)
Query: 55 SEDTKALN-QLSSLTQEGKEM----ASVLYTYRSCVKALPQLPDSM--KQSQADLYLETY 107
SED K L + +LTQ+ K M S++ +Y+S +L D QSQ + LE+
Sbjct: 758 SEDVKRLTANVETLTQDSKAMKQSFTSLVNSYQSISNLYHELRDDHVNMQSQNNTLLESE 817
Query: 108 QVLDLEMSRLREIQRWQASASSKLATDMQRFSRPERRINGPTISHLWSMLKLFDVLVQLD 167
L + L + KL M + E ++ S LK + + LD
Sbjct: 818 SKLKTDCENLTQQNMTLIDNVQKL---MHKHVNQESKV---------SELKEVNGKLSLD 865
Query: 168 HLKNAKASIPNDFSWYKRTFTQVSGQWQDTDSMREELDDLQIFLSTRWAILLNLHVE 224
LKN ++S+ S + TQ++ ++ DS+ +E L L + A LH E
Sbjct: 866 -LKNLRSSLNVAISDNDQILTQLAELSKNYDSLEQESAQLNSGLKSLEAEKQLLHTE 921
>CNRB_CANFA (P33726) Rod cGMP-specific 3',5'-cyclic
phosphodiesterase beta-subunit (EC 3.1.4.17) (GMP-PDE
beta)
Length = 856
Score = 32.0 bits (71), Expect = 10.0
Identities = 29/108 (26%), Positives = 51/108 (46%), Gaps = 18/108 (16%)
Query: 435 YEKVVRYNYSAEERKALVELVSCIKS-----VGSMMQRCDTLVADALWETIHAEVQDFVQ 489
++K+V + + E+RK+ VE +S + + MM CD WE VQ
Sbjct: 679 FQKIVDESKNYEDRKSWVEYLSLETTRKEIVMAMMMTACDLSAITKPWE---------VQ 729
Query: 490 NTLASMLRTTFRKKKDLSRILSDMRTLSADWMANTNKSESELQSSQHG 537
+ +A ++ F ++ DL R + D + + M + NK+ +EL Q G
Sbjct: 730 SKVALLVAAEFWEQGDLERTVLDQQPIP---MMDRNKA-AELPKLQVG 773
>CNRB_BOVIN (P23439) Rod cGMP-specific 3',5'-cyclic
phosphodiesterase beta-subunit (EC 3.1.4.17) (GMP-PDE
beta)
Length = 853
Score = 32.0 bits (71), Expect = 10.0
Identities = 29/108 (26%), Positives = 51/108 (46%), Gaps = 18/108 (16%)
Query: 435 YEKVVRYNYSAEERKALVELVSCIKS-----VGSMMQRCDTLVADALWETIHAEVQDFVQ 489
++K+V + + E+RK+ VE +S + + MM CD WE VQ
Sbjct: 679 FQKIVDESKNYEDRKSWVEYLSLETTRKEIVMAMMMTACDLSAITKPWE---------VQ 729
Query: 490 NTLASMLRTTFRKKKDLSRILSDMRTLSADWMANTNKSESELQSSQHG 537
+ +A ++ F ++ DL R + D + + M + NK+ +EL Q G
Sbjct: 730 SKVALLVAAEFWEQGDLERTVLDQQPIP---MMDRNKA-AELPKLQVG 773
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.331 0.140 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 122,608,170
Number of Sequences: 164201
Number of extensions: 4780669
Number of successful extensions: 13779
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 13771
Number of HSP's gapped (non-prelim): 24
length of query: 1157
length of database: 59,974,054
effective HSP length: 121
effective length of query: 1036
effective length of database: 40,105,733
effective search space: 41549539388
effective search space used: 41549539388
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 71 (32.0 bits)
Medicago: description of AC146587.10