BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146585.9 - phase: 0
(724 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LCFH_HAEIN (P44446) Putative long-chain-fatty-acid--CoA ligase (... 199 3e-50
LCF1_HUMAN (P33121) Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.... 197 8e-50
LCF1_CAVPO (Q9JID6) Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.... 192 3e-48
LCF5_HUMAN (Q9ULC5) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.... 191 6e-48
LCF3_YEAST (P39002) Long-chain-fatty-acid--CoA ligase 3 (EC 6.2.... 190 1e-47
LCF2_MOUSE (P41216) Long-chain-fatty-acid--CoA ligase 2 (EC 6.2.... 188 4e-47
LCF5_RAT (O88813) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.1.... 186 2e-46
LCF4_YEAST (P47912) Long-chain-fatty-acid--CoA ligase 4 (EC 6.2.... 182 2e-45
LCF1_YEAST (P30624) Long-chain-fatty-acid--CoA ligase 1 (EC 6.2.... 179 2e-44
LCF5_MOUSE (Q8JZR0) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.... 179 3e-44
LCF2_RAT (P18163) Long-chain-fatty-acid--CoA ligase, liver isozy... 177 1e-43
LCF6_HUMAN (Q9UKU0) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.... 163 2e-39
FD11_MYCTU (Q10776) Putative fatty-acid--CoA ligase fadD11 (EC 6... 163 2e-39
LCF6_MOUSE (Q91WC3) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.... 161 6e-39
LCF6_RAT (P33124) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.1.... 159 2e-38
LCF2_YEAST (P39518) Long-chain-fatty-acid--CoA ligase 2 (EC 6.2.... 134 8e-31
LCFA_BACSU (P94547) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.... 106 2e-22
LCFA_SALTY (P63521) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.... 103 2e-21
LCFA_SALTI (P63522) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.... 103 2e-21
LCFA_ECOLI (P29212) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.... 102 4e-21
>LCFH_HAEIN (P44446) Putative long-chain-fatty-acid--CoA ligase (EC
6.2.1.3) (Long-chain acyl-CoA synthetase) (LACS)
Length = 607
Score = 199 bits (505), Expect = 3e-50
Identities = 165/617 (26%), Positives = 284/617 (45%), Gaps = 72/617 (11%)
Query: 112 NQLEQAILDYAEGLRVIGVRPDEKLALFADNSCRWLVADQGMMASGAINVVRGSRSSVEE 171
NQL +A+L + + +K+A+FA N RW + D + AI V + ++ ++
Sbjct: 53 NQLSRALLAH-------NIDVQDKIAIFAHNMERWTIVDIATLQIRAITVPIYATNTAQQ 105
Query: 172 LLQIYNHSESVALAVDGPEMFNRIAKPFYSKTGMRFIILLWGEKSDLNLIAEENKEVPIF 231
I NH++ L V E +++ + + ++ I+ + KS + L ++ P+
Sbjct: 106 AEFILNHADVKILFVGDQEQYDQTLEIAHHCPKLQKIVAM---KSTIQL-----QQDPLS 157
Query: 232 SFME-VIDLGRESRMALSDSHEASQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNL 290
E I G ++ E +QR + ++ D+ T+IYTSGTTG PKGVML + NL
Sbjct: 158 CTWESFIKTGSNAQQ-----DELTQRLNQKQLS--DLFTIIYTSGTTGEPKGVMLDYANL 210
Query: 291 LHQIKNLWDTVPAEVGDRFLSMLPPWHAYERACEYFIFTCGIEQVYTTVRN-LKDDLGRY 349
HQ++ ++ D LS LP H +ERA +I G Y N ++ L
Sbjct: 211 AHQLETHDLSLNVTDQDISLSFLPFSHIFERAWAAYILHRGAILCYLEDTNQVRSALTEI 270
Query: 350 QPHYMISVPLVFETLYSGIQKQISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNV 409
+P M +VP +E +Y+ + ++ +P +R+++ I V Y + + N
Sbjct: 271 RPTLMCAVPRFYEKIYAAVLDKVQKAPKLRQIMFHWAISVGQKYFDLR--------ANNK 322
Query: 410 KQPSIVNSMLDCLWARIIATILFPIHLLAIKFVYSKIHSAIG-LSKAGISGGGSLPLEVD 468
P ++ LA K V SK+ +G K GG L +
Sbjct: 323 AIPFLLKKQF----------------ALADKLVLSKLRQLLGGRIKMMPCGGAKLEPAIG 366
Query: 469 KFFEAIGVKVQNGYGLTETSPVIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGS 528
FF AIG+ ++ GYG+TET+ ++ S+G + E K+ E E+L
Sbjct: 367 LFFHAIGINIKLGYGMTETTATVSCWHDFQFNPNSIGTLMPKAEVKI--GENNEIL---- 420
Query: 529 KGILKVRGPPVMNGYYKNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDG 588
VRG VM GYYK P T QA +DG+L TGD G + G + +
Sbjct: 421 -----VRGGMVMKGYYKKPEETAQAFTEDGFLKTGDAG----------EFDEQGNLFITD 465
Query: 589 RAKDTIVLSTGENVEPAELEEAAMRSSIIQQIVVIGQDKRRLGAIIVPNSEEVLKVAREL 648
R K+ + S G+ + P +E + I+QI +I K+ + A+IVP + + + A++L
Sbjct: 466 RIKELMKTSNGKYIAPQYIESKIGKDKFIEQIAIIADAKKYVSALIVPCFDSLEEYAKQL 525
Query: 649 SIIDSISSNVVSEEKVLNLIYKELKTWMSESPF--QIGPILLVNEPFTIDNGLMTPTMKI 706
+I ++ +L + + E Q+ L+++ F+I G +TPT+K+
Sbjct: 526 NIKYHDRLELLKNSDILKMFEHRINAVQKELAHFEQVKKFTLLSQAFSIKLGEITPTLKL 585
Query: 707 RRDRVVAKYKEQIDDLY 723
RR ++ +Y++QI+ +Y
Sbjct: 586 RRKVILERYRKQIEAMY 602
>LCF1_HUMAN (P33121) Long-chain-fatty-acid--CoA ligase 1 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 1) (LACS 1)
(Palmitoyl-CoA ligase 1) (Long-chain fatty acid CoA
ligase 2) (Long-chain acyl-CoA synthetase 2) (LACS 2)
(Acyl-CoA synthetase 1) (ACS1) (Pal
Length = 698
Score = 197 bits (501), Expect = 8e-50
Identities = 175/608 (28%), Positives = 275/608 (44%), Gaps = 76/608 (12%)
Query: 132 PDEKLALFADNSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEM 191
PD+ + +FA N W++ +QG A + V E + I N +E + VD PE
Sbjct: 146 PDQFIGIFAQNRPEWVIIEQGCFAYSMVIVPLYDTLGNEAITYIVNKAELSLVFVDKPEK 205
Query: 192 FNRIAKPFYSKT--GMRFIILLWGEKSDLNLIAEENKEVPIFSFMEVIDLGRESRMALSD 249
+ + +K G++ I+++ S+L + + V + S + DLGR +R
Sbjct: 206 AKLLLEGVENKLIPGLKIIVVMDAYGSEL-VERGQRCGVEVTSMKAMEDLGRANRRKPKP 264
Query: 250 SHEASQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNLLHQ----IKNLWDTVPAEV 305
+D+A + +TSGTTGNPKG M+THRN++ +K +TV
Sbjct: 265 PAP------------EDLAVICFTSGTTGNPKGAMVTHRNIVSDCSAFVKATENTVNPCP 312
Query: 306 GDRFLSMLPPWHAYERACEYFIFTCGIEQVYTT--VRNLKDDLGRYQPHYMISVPLVFET 363
D +S LP H +ER E + G + + +R L DDL QP VP +
Sbjct: 313 DDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTVFPVVPRLLNR 372
Query: 364 LYSGIQKQISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKC-LTRNVKQPSIVNSMLDCL 422
++ I Q +T T R L + ++ E + + RN + L
Sbjct: 373 MFDRIFGQANT----------TLKRWLLDFASKRKEAELRSGIIRN-----------NSL 411
Query: 423 WARIIATILFPIHLLAIKFVYSKIHSAIG-LSKAGISGGGSLPLEVDKFFEA-IGVKVQN 480
W R+I + K+ S++G + ++G + V F A +G +
Sbjct: 412 WDRLI---------------FHKVQSSLGGRVRLMVTGAAPVSATVLTFLRAALGCQFYE 456
Query: 481 GYGLTETSPVIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVM 540
GYG TE + P G VG P+ K+VD E + +G + V+GP V
Sbjct: 457 GYGQTECTAGCCLTMPGDWTAGHVGAPMPCNLIKLVDVEEMNYMAAEGEGEVCVKGPNVF 516
Query: 541 NGYYKNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGE 600
GY K+P T +ALDKDGWL+TGD+G P +G + + R K L+ GE
Sbjct: 517 QGYLKDPAKTAEALDKDGWLHTGDIGKWLP----------NGTLKIIDRKKHIFKLAQGE 566
Query: 601 NVEPAELEEAAMRSSIIQQIVVIGQDKRR-LGAIIVPNSEEVLKVARELSIIDSISSNVV 659
+ P ++E MRS + Q+ V G+ + L AI+VP+ E + A++ S +
Sbjct: 567 YIAPEKIENIYMRSEPVAQVFVHGESLQAFLIAIVVPDVETLCSWAQKRGFEGSF-EELC 625
Query: 660 SEEKVLNLIYKELKTWMSES---PF-QIGPILLVNEPFTIDNGLMTPTMKIRRDRVVAKY 715
+ V I +++ +S PF Q+ I L E F+IDNGL+TPTMK +R + +
Sbjct: 626 RNKDVKKAILEDMVRLGKDSGLKPFEQVKGITLHPELFSIDNGLLTPTMKAKRPELRNYF 685
Query: 716 KEQIDDLY 723
+ QIDDLY
Sbjct: 686 RSQIDDLY 693
>LCF1_CAVPO (Q9JID6) Long-chain-fatty-acid--CoA ligase 1 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 1) (LACS 1)
(Palmitoyl-CoA ligase)
Length = 698
Score = 192 bits (488), Expect = 3e-48
Identities = 173/638 (27%), Positives = 284/638 (44%), Gaps = 80/638 (12%)
Query: 105 PPSTITYNQLEQAILDYAEGLRVIGVR--PDEKLALFADNSCRWLVADQGMMASGAINVV 162
P ++Y Q+E L G + PD+ + +FA N W++ +Q A + V
Sbjct: 117 PYEWLSYKQVEDLSECIGSALLQKGFQASPDQFIGIFAQNRPEWVIIEQACFAYSMVVVP 176
Query: 163 RGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYSKT--GMRFIILLWGEKSDLNL 220
+ + I N +E + D PE + + +K G++ I+++ S+L
Sbjct: 177 LYDTLGADAITYIVNKAELSVIFADKPEKARILLESVENKLTPGLKIIVVMDSYGSEL-- 234
Query: 221 IAEENKE--VPIFSFMEVIDLGRESRMALSDSHEASQRYVYEAINSDDIATLIYTSGTTG 278
E+ K+ V + S + LGR +R DD+A + +TSGTTG
Sbjct: 235 -VEQGKKCGVEVISLKAMEGLGRANRRKPKPPEP------------DDLAVICFTSGTTG 281
Query: 279 NPKGVMLTHRNLLHQ----IKNLWDTVPAEVGDRFLSMLPPWHAYERACEYFIFTCGIEQ 334
NPKG M+TH+N++ +K + D +S LP H YE+ + + G +
Sbjct: 282 NPKGAMITHKNVVSDCSAFVKATEKALVLNASDIHISFLPLAHMYEQLLQCVMLCHGAKI 341
Query: 335 VYTT--VRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQISTSPPVRKLVALTFIRVSLA 392
+ +R L DDL QP VP + ++ I Q +T T R L
Sbjct: 342 GFFQGDIRLLMDDLKALQPTIFPVVPRLLNRMFDRIFAQANT----------TVKRWLLD 391
Query: 393 YMEYKRIYEGKC-LTRNVKQPSIVNSMLDCLWARIIATILFPIHLLAIKFVYSKIHSAIG 451
+ ++ E + + RN NS+ D K ++ KI S++G
Sbjct: 392 FASKRKEAELRSGIIRN-------NSVWD-------------------KLIFHKIQSSLG 425
Query: 452 LS-KAGISGGGSLPLEVDKFFEA-IGVKVQNGYGLTETSPVIAARRPRCNVIGSVGHPVQ 509
+ ++G + V F A +G + GYG TE + + P G VG P+
Sbjct: 426 GKVRLMVTGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCSLSVPGDWTAGHVGAPMP 485
Query: 510 HTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQALDKDGWLNTGDLGWIA 569
K+VD E + +G + V+GP V GY K+P T +ALDKDGWL+TGD+G
Sbjct: 486 CNFIKLVDVEEMNYMAAMGEGEVCVKGPNVFKGYLKDPAKTAEALDKDGWLHTGDIGKWL 545
Query: 570 PHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRSSIIQQIVVIGQDKRR 629
P +G + + R K L+ GE + P ++E +RS + Q+ V G+ +
Sbjct: 546 P----------NGTLKIIDRKKHIFKLAQGEYIAPEKIENIYVRSEPVAQVFVHGESLQA 595
Query: 630 -LGAIIVPNSEEVLKVARELSI---IDSISSNVVSEEKVLNLIYKELKTWMSESPFQIGP 685
L AI+VP++E + AR+ + + N ++ +L + + K +S Q+
Sbjct: 596 FLIAIVVPDAESLASWARKRGFEGSFEELCRNKDVKKAILEDMVRIGKDSGLKSFEQVRG 655
Query: 686 ILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQIDDLY 723
I L E F++DNGL+TPTMK +R + ++ QID+LY
Sbjct: 656 IALHPELFSVDNGLLTPTMKAKRPELRNYFRSQIDELY 693
>LCF5_HUMAN (Q9ULC5) Long-chain-fatty-acid--CoA ligase 5 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 5) (LACS 5)
Length = 683
Score = 191 bits (485), Expect = 6e-48
Identities = 161/613 (26%), Positives = 280/613 (45%), Gaps = 84/613 (13%)
Query: 132 PDEKLALFADNSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPE- 190
PD+ + +FA N W++++ + V E ++ I N ++ + D P+
Sbjct: 131 PDQFVGIFAQNRPEWIISELACYTYSMVAVPLYDTLGPEAIVHIVNKADIAMVICDTPQK 190
Query: 191 ---MFNRIAKPFYSKTGMRFIILLWGEKSDLNLIAEENKEVPIFSFMEVIDLGRES-RMA 246
+ + K F ++ IIL+ DL E++ + I S + +LG+E R
Sbjct: 191 ALVLIGNVEKGF--TPSLKVIILMDPFDDDLKQRGEKSG-IEILSLYDAENLGKEHFRKP 247
Query: 247 LSDSHEASQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNL-------LHQIKNLWD 299
+ S E D++ + +TSGTTG+PKG M+TH+N+ L +++ ++
Sbjct: 248 VPPSPE-------------DLSVICFTSGTTGDPKGAMITHQNIVSNAAAFLKCVEHAYE 294
Query: 300 TVPAEVGDRFLSMLPPWHAYERACEYFIFTCGIEQVY--TTVRNLKDDLGRYQPHYMISV 357
P +V +S LP H +ER + +++CG + +R L DD+ +P +V
Sbjct: 295 PTPDDVA---ISYLPLAHMFERIVQAVVYSCGARVGFFQGDIRLLADDMKTLKPTLFPAV 351
Query: 358 PLVFETLYSGIQKQISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNS 417
P + +Y +Q + T P++K L + VS + E + K + R+
Sbjct: 352 PRLLNRIYDKVQNEAKT--PLKKF--LLKLAVSSKFKELQ-----KGIIRH--------- 393
Query: 418 MLDCLWARIIATILFPIHLLAIKFVYSKIHSAI-GLSKAGISGGGSLPLEVDKFFE-AIG 475
D W K +++KI ++ G + ++G + V FF A+G
Sbjct: 394 --DSFWD---------------KLIFAKIQDSLGGRVRVIVTGAAPMSTSVMTFFRAAMG 436
Query: 476 VKVQNGYGLTETSPVIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVR 535
+V YG TE + P G VG P+ K+ D ++G + ++
Sbjct: 437 CQVYEAYGQTECTGGCTFTLPGDWTSGHVGVPLACNYVKLEDVADMNYFTVNNEGEVCIK 496
Query: 536 GPPVMNGYYKNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIV 595
G V GY K+P T +ALD DGWL+TGD+G P +G + + R K+
Sbjct: 497 GTNVFKGYLKDPEKTQEALDSDGWLHTGDIGRWLP----------NGTLKIIDRKKNIFK 546
Query: 596 LSTGENVEPAELEEAAMRSSIIQQIVVIGQDKR-RLGAIIVPNSEEVLKVARELSI---I 651
L+ GE + P ++E RS + QI V G+ R L ++VP+++ + A +L +
Sbjct: 547 LAQGEYIAPEKIENIYNRSQPVLQIFVHGESLRSSLVGVVVPDTDVLPSFAAKLGVKGSF 606
Query: 652 DSISSNVVSEEKVLNLIYKELKTWMSESPFQIGPILLVNEPFTIDNGLMTPTMKIRRDRV 711
+ + N V E +L + K K ++ Q+ I L EPF+I+NGL+TPT+K +R +
Sbjct: 607 EELCQNQVVREAILEDLQKIGKESGLKTFEQVKAIFLHPEPFSIENGLLTPTLKAKRGEL 666
Query: 712 VAKYKEQIDDLYK 724
++ QID LY+
Sbjct: 667 SKYFRTQIDSLYE 679
>LCF3_YEAST (P39002) Long-chain-fatty-acid--CoA ligase 3 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 3) (Fatty acid
activator 3)
Length = 694
Score = 190 bits (482), Expect = 1e-47
Identities = 172/656 (26%), Positives = 288/656 (43%), Gaps = 96/656 (14%)
Query: 105 PPSTITYNQLEQAILDYAEGLRVIGVRP--DEKLALFADNSCRWLVADQGMMASGAINVV 162
P +TY +L + D GL IG++P + K +FA S +W+ G ++ G V
Sbjct: 96 PYKMMTYQELIWVMHDMGRGLAKIGIKPNGEHKFHIFASTSHKWMKIFLGCISQGIPVVT 155
Query: 163 RGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYSKTGMRFII------------- 209
L+ +ES A+ D ++ ++ P S ++F+I
Sbjct: 156 AYDTLGESGLIHSMVETESAAIFTDN-QLLAKMIVPLQSAKDIKFLIHNEPIDPNDRRQN 214
Query: 210 --LLWGEKSDLNLIAEENKEVPIFSFMEVIDLGRESRMALSDSHEASQRYVYEAINSDDI 267
L K +N I E ++ I+SF EV+ +G++S+ + H + D+
Sbjct: 215 GKLYKAAKDAINKIREVRPDIKIYSFEEVVKIGKKSKDEVK-LHPPEPK---------DL 264
Query: 268 ATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDTVPAEVG--DRFLSMLPPWHAYERACEY 325
A ++YTSG+ PKGV+LTH N++ I + V +G DR LS LP H +E E+
Sbjct: 265 ACIMYTSGSISAPKGVVLTHYNIVSGIAGVGHNVFGWIGSTDRVLSFLPLAHIFELVFEF 324
Query: 326 FIF----TCGIEQVYT----TVRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQISTSPP 377
F G V T + RN K DL ++P MI V V+ET+ I ++IS P
Sbjct: 325 EAFYWNGILGYGSVKTLTNTSTRNCKGDLVEFKPTIMIGVAAVWETVRKAILEKISDLTP 384
Query: 378 VRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWARIIATILFPIHLL 437
V + + + AY ++K+ S+ P
Sbjct: 385 VLQKIFWS------AY--------------SMKEKSV------------------PCTGF 406
Query: 438 AIKFVYSKIHSAIGLS-KAGISGGGSLPLEVDKFFEAIGVKVQNGYGLTETSPVIAARRP 496
+ V+ K+ A G K ++GG ++ ++ KFF + + GYGLTET P
Sbjct: 407 LSRMVFKKVRQATGGHLKYIMNGGSAISIDAQKFFSIVLCPMIIGYGLTETVANACVLEP 466
Query: 497 RCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQALDK 556
G VG V K+VD + ++G L ++G PV + YYKNP+ T +
Sbjct: 467 DHFEYGIVGDLVGSVTAKLVDVKDLGYYAKNNQGELLLKGAPVCSEYYKNPIETAVSFTY 526
Query: 557 DGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRSSI 616
DGW TGD+ P G + + R K+ + GE + +LE +S
Sbjct: 527 DGWFRTGDIVEWTP----------KGQLKIIDRRKNLVKTLNGEYIALEKLESVYRSNSY 576
Query: 617 IQQIVVIGQDKR-RLGAIIVPNSEEVLKVARELSIIDSIS--SNVVSEEKVLNLIYKELK 673
++ I V + R + I+VPN + K A +L I+ N + ++ + N ++KE+
Sbjct: 577 VKNICVYADESRVKPVGIVVPNPGPLSKFAVKLRIMKKGEDIENYIHDKALRNAVFKEMI 636
Query: 674 TWMSESPFQIG-----PILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQIDDLYK 724
++S +G I+ +E +T +NG +T K++R ++A K +++ +YK
Sbjct: 637 A-TAKSQGLVGIELLCGIVFFDEEWTPENGFVTSAQKLKRREILAAVKSEVERVYK 691
>LCF2_MOUSE (P41216) Long-chain-fatty-acid--CoA ligase 2 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 2) (LACS 2)
Length = 699
Score = 188 bits (478), Expect = 4e-47
Identities = 172/640 (26%), Positives = 283/640 (43%), Gaps = 80/640 (12%)
Query: 103 HHPPSTITYNQLEQAILDYAEGLRVIGVRP--DEKLALFADNSCRWLVADQGMMASGAIN 160
+ P I+Y ++ + GL G +P ++ + LF+ N W++ +QG + +
Sbjct: 116 NQPYEWISYKEVAELAECIGSGLIQKGFKPCSEQFIGLFSQNRPEWVIVEQGCFSYSMVV 175
Query: 161 VVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYSKTG--MRFIILLWGEKSDL 218
V + + I N +E + D PE + + +K ++ I+++ SDL
Sbjct: 176 VPLYDTLGADAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYGSDL 235
Query: 219 NLIAEENKE--VPIFSFMEVIDLGRESRMALSDSHEASQRYVYEAINSDDIATLIYTSGT 276
E K+ V I S + DLGR +R+ +D+A + +TSGT
Sbjct: 236 ---VERGKKCGVEIISLKALEDLGRVNRVKPKPPEP------------EDLAIICFTSGT 280
Query: 277 TGNPKGVMLTHRNLLHQ----IKNLWDTVPAEVGDRFLSMLPPWHAYERACEYFIFTCGI 332
TGNPKG M+TH+N+++ IK A D +S LP H +E E + G
Sbjct: 281 TGNPKGAMITHQNIINDCSGFIKATESAFIASTDDVLISFLPLAHMFETVVECVMLCHGA 340
Query: 333 EQVYTT--VRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQISTSPPVRKLVALTFIRVS 390
+ + +R L DDL QP VP + ++ I Q +TS
Sbjct: 341 KIGFFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTS--------------- 385
Query: 391 LAYMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWARIIATILFPIHLLAIKFVYSKIHSAI 450
+ + K +V+ + N+ L W ++I + KI S++
Sbjct: 386 --LKRWLLDFASKRKEADVRSGIVRNNSL---WDKLI---------------FHKIQSSL 425
Query: 451 GLS-KAGISGGGSLPLEVDKFFE-AIGVKVQNGYGLTETSPVIAARRPRCNVIGSVGHPV 508
G + I+G + V F A+G + GYG TE + P G VG P+
Sbjct: 426 GGKVRLMITGAAPVSATVLTFLRTALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPM 485
Query: 509 QHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQALDKDGWLNTGDLGWI 568
K+VD E L +G + V+G V GY K+P T +ALDKDGWL+TGD+G
Sbjct: 486 PCNYVKLVDVEEMNYLASKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKW 545
Query: 569 APHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRSSIIQQIVVIGQDKR 628
P +G + + R K L+ GE + P ++E +RS + Q+ V G+ +
Sbjct: 546 LP----------NGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQ 595
Query: 629 R-LGAIIVPNSEEVLKVARELSI---IDSISSNVVSEEKVLNLIYKELKTWMSESPF-QI 683
L A++VP+ E + A++ + + + N + +L+ + K L PF Q+
Sbjct: 596 AFLIAVVVPDVESLPSWAQKRGLQGSFEELCRNKDINKAILDDLLK-LGKEAGLKPFEQV 654
Query: 684 GPILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQIDDLY 723
I + E F+IDNGL+TPT+K +R + ++ QID+LY
Sbjct: 655 KGIAVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELY 694
>LCF5_RAT (O88813) Long-chain-fatty-acid--CoA ligase 5 (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase 5) (LACS 5)
Length = 683
Score = 186 bits (472), Expect = 2e-46
Identities = 165/638 (25%), Positives = 280/638 (43%), Gaps = 74/638 (11%)
Query: 103 HHPPSTITYNQLEQAILDYAEGLRVIGVRP--DEKLALFADNSCRWLVADQGMMASGAIN 160
+ P I+Y Q+ L G +P D+ + +FA N W++++ +
Sbjct: 100 NQPYKWISYKQVSDRAEYLGSCLLHKGYKPSQDQFIGIFAQNRPEWVISELACYTYSMVA 159
Query: 161 VVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYSKT--GMRFIILLWGEKSDL 218
V E ++ + N ++ + D P+ + + G++ +IL+ DL
Sbjct: 160 VPLYDTLGAEAIIYVINRADISVVICDTPQKATMLIENVEKDLTPGLKTVILMDPFDDDL 219
Query: 219 NLIAEENKEVPIFSFMEVIDLGRESRMALSDSHEASQRYVYEAINSDDIATLIYTSGTTG 278
+ E + + S + +LG+E+ N +D++ + +TSGTTG
Sbjct: 220 -MKRGEKCGIEMLSLHDAENLGKENFKKPMPP------------NPEDLSVICFTSGTTG 266
Query: 279 NPKGVMLTHRNLLHQ----IKNLWDTVPAEVGDRFLSMLPPWHAYERACEYFIFTCG--I 332
+PKG MLTH+N++ +K L D +S LP H +ER + IF+CG I
Sbjct: 267 DPKGAMLTHQNIVSNMAAFLKFLEPIFQPTPEDVTISYLPLAHMFERLVQGVIFSCGGKI 326
Query: 333 EQVYTTVRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQISTSPPVRKLVALTFIRVSLA 392
+R L DD+ +P +VP + +Y +Q + T P++K + ++LA
Sbjct: 327 GFFQGDIRLLPDDMKALKPTVFPTVPRLLNRVYDKVQNEAKT--PLKKFL------LNLA 378
Query: 393 YMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWARIIATILFPIHLLAIKFVYSKIHSAI-G 451
+ + RN LW K V+SKI S++ G
Sbjct: 379 IISKFNEVRNGIIRRN------------SLWD---------------KLVFSKIQSSLGG 411
Query: 452 LSKAGISGGGSLPLEVDKFFE-AIGVKVQNGYGLTETSPVIAARRPRCNVIGSVGHPVQH 510
+ I+G + V FF A+G V YG TE + + P G VG PV
Sbjct: 412 KVRLMITGAAPISTPVLTFFRAAMGCWVFEAYGQTECTAGCSITSPGDWTAGHVGTPVSC 471
Query: 511 TEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQALDKDGWLNTGDLGWIAP 570
K+ D ++G + ++G V GY K+P T + LDKDGWL+TGD+G P
Sbjct: 472 NFVKLEDVADMNYFSVNNEGEICIKGNNVFKGYLKDPEKTQEVLDKDGWLHTGDIGRWLP 531
Query: 571 HHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRSSIIQQIVVIGQDKRR- 629
+G + + R K+ L+ GE + P ++E RS I Q+ V G+ R
Sbjct: 532 ----------NGTLKIIDRKKNIFKLAQGEYIAPEKIENVYSRSRPILQVFVHGESLRSF 581
Query: 630 LGAIIVPNSEEVLKVARELSI---IDSISSNVVSEEKVLNLIYKELKTWMSESPFQIGPI 686
L ++VP+ E + A ++ + + + N ++ +L + K K +S Q+ I
Sbjct: 582 LIGVVVPDPESLPSFAAKIGVKGSFEELCQNQCVKKAILEDLQKVGKEGGLKSFEQVKSI 641
Query: 687 LLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQIDDLYK 724
+ EPF+I+NGL+TPT+K +R + ++ QI LY+
Sbjct: 642 FVHPEPFSIENGLLTPTLKAKRVELAKFFQTQIKSLYE 679
>LCF4_YEAST (P47912) Long-chain-fatty-acid--CoA ligase 4 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 4) (Fatty acid
activator 4)
Length = 694
Score = 182 bits (463), Expect = 2e-45
Identities = 165/656 (25%), Positives = 288/656 (43%), Gaps = 96/656 (14%)
Query: 105 PPSTITYNQLEQAILDYAEGLRVIGVRP--DEKLALFADNSCRWLVADQGMMASGAINVV 162
P T+TY ++ + D GL IGV+P + K +FA S +W+ G M+ G V
Sbjct: 96 PYITMTYEEMICVMHDIGRGLIKIGVKPNGENKFHIFASTSHKWMKTFLGCMSQGIPVVT 155
Query: 163 RGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYSKTGMRFII------------- 209
L+ ++SVA+ D ++ +++A P + ++F+I
Sbjct: 156 AYDTLGESGLIHSMVETDSVAIFTDN-QLLSKLAVPLKTAKNVKFVIHNEPIDPSDKRQN 214
Query: 210 --LLWGEKSDLNLIAEENKEVPIFSFMEVIDLGRESRMALSDSHEASQRYVYEAINSDDI 267
L K ++ I E ++ I+SF E+I++G++++ E + +D
Sbjct: 215 GKLYKAAKDAVDKIKEVRPDIKIYSFDEIIEIGKKAK------DEVELHFP----KPEDP 264
Query: 268 ATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDTVPAEVG--DRFLSMLPPWHAYERACEY 325
A ++YTSG+TG PKGV+LTH N++ I + V +G DR ++ LP H +E E+
Sbjct: 265 ACIMYTSGSTGTPKGVVLTHYNIVAGIGGVGHNVIGWIGPTDRIIAFLPLAHIFELTFEF 324
Query: 326 FIF----TCGIEQVY----TTVRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQISTSPP 377
F G V T+ RN + DL ++P M+ V V+ET+ GI +I+ P
Sbjct: 325 EAFYWNGILGYANVKTLTPTSTRNCQGDLMEFKPTVMVGVAAVWETVRKGILAKINELPG 384
Query: 378 VRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWARIIATILFPIHLL 437
+ + T +Y K RN+ +++ +
Sbjct: 385 WSQTLFWT-------------VYALK--ERNIPCSGLLSGL------------------- 410
Query: 438 AIKFVYSKIHSAIGLS-KAGISGGGSLPLEVDKFFEAIGVKVQNGYGLTETSPVIAARRP 496
++ +I A G + + ++GG ++ ++ KF + + GYGLTE P
Sbjct: 411 ----IFKRIREATGGNLRFILNGGSAISIDAQKFLSNLLCPMLIGYGLTEGVANACVLEP 466
Query: 497 RCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQALDK 556
G G V K+VD E ++G L +G P+ + YYKNP T A
Sbjct: 467 EHFDYGIAGDLVGTITAKLVDVEDLGYFAKNNQGELLFKGAPICSEYYKNPEETAAAFTD 526
Query: 557 DGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRSSI 616
DGW TGD+ P G + + R K+ + GE + +LE +
Sbjct: 527 DGWFRTGDIAEWTP----------KGQVKIIDRKKNLVKTLNGEYIALEKLESIYRSNPY 576
Query: 617 IQQIVVIG-QDKRRLGAIIVPNSEEVLKVARELSII---DSISSNVVSEEKVLNLIYKEL 672
+Q I V ++K + I+VPN + K+A EL I+ + + S + E+K+ + + K++
Sbjct: 577 VQNICVYADENKVKPVGIVVPNLGHLSKLAIELGIMVPGEDVES-YIHEKKLQDAVCKDM 635
Query: 673 KTWMSESPFQ----IGPILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQIDDLYK 724
+ + I+ E +T +NGL+T K++R ++A K ++ +YK
Sbjct: 636 LSTAKSQGLNGIELLCGIVFFEEEWTPENGLVTSAQKLKRRDILAAVKPDVERVYK 691
>LCF1_YEAST (P30624) Long-chain-fatty-acid--CoA ligase 1 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 1) (Fatty acid
activator 1)
Length = 700
Score = 179 bits (454), Expect = 2e-44
Identities = 186/725 (25%), Positives = 299/725 (40%), Gaps = 116/725 (16%)
Query: 50 KCSPFLESSMLVGNNNNNNNAAVDSSHEWKVVPDIWRSSA---EKYGDKVALVDQ----- 101
KCS E + N N+NA W+ V +I S +K K V++
Sbjct: 38 KCSTVYEFVLECFQKNKNSNAM-----GWRDVKEIHEESKSVMKKVDGKETSVEKKWMYY 92
Query: 102 ----YHHPPSTITYNQLEQAILDYAEGLRVIGVRP--DEKLALFADNSCRWLVADQGMMA 155
YH+ +++QL + + GL IG++P D+KL L+A S +W+ G +
Sbjct: 93 ELSHYHYN----SFDQLTDIMHEIGRGLVKIGLKPNDDDKLHLYAATSHKWMKMFLGAQS 148
Query: 156 SGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYSKTGMRFIILLWGEK 215
G V + L+ + S A+ D + + KP + +++II
Sbjct: 149 QGIPVVTAYDTLGEKGLIHSLVQTGSKAIFTDN-SLLPSLIKPVQAAQDVKYIIHFDSIS 207
Query: 216 SD---------------LNLIAEENKEVPIFSFMEVIDLGRESRMALSDSHEASQRYVYE 260
S+ +N I E ++ FSF +++ LG+ES + D H +
Sbjct: 208 SEDRRQSGKIYQSAHDAINRIKEVRPDIKTFSFDDILKLGKESCNEI-DVHPPGK----- 261
Query: 261 AINSDDIATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDTVPAEVG--DRFLSMLPPWHA 318
DD+ ++YTSG+TG PKGV+L H N++ + V VG DR + LP H
Sbjct: 262 ----DDLCCIMYTSGSTGEPKGVVLKHSNVVAGVGGASLNVLKFVGNTDRVICFLPLAHI 317
Query: 319 YERACEYFIFTCGIEQVYTT--------VRNLKDDLGRYQPHYMISVPLVFETLYSGIQK 370
+E E F G Y T VRN + DL ++P M+ V V+ET+ GI
Sbjct: 318 FELVFELLSFYWGACIGYATVKTLTSSSVRNCQGDLQEFKPTIMVGVAAVWETVRKGILN 377
Query: 371 QISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWARIIATI 430
QI P + K + T L M+ I G L
Sbjct: 378 QIDNLPFLTKKIFWTAYNTKL-NMQRLHIPGGGALG------------------------ 412
Query: 431 LFPIHLLAIKFVYSKIHSAI-GLSKAGISGGGSLPLEVDKFFEAIGVKVQNGYGLTETSP 489
V+ KI +A G + ++GG + + +F + + GYGLTET
Sbjct: 413 ---------NLVFKKIRTATGGQLRYLLNGGSPISRDAQEFITNLICPMLIGYGLTETCA 463
Query: 490 VIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLA 549
P +G G K+VD E ++G + + G V YYKN
Sbjct: 464 STTILDPANFELGVAGDLTGCVTVKLVDVEELGYFAKNNQGEVWITGANVTPEYYKNEEE 523
Query: 550 TNQALDKDGWLNTGDLG-WIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELE 608
T+QAL DGW TGD+G W ++G + + R K+ + GE + +LE
Sbjct: 524 TSQALTSDGWFKTGDIGEW-----------EANGHLKIIDRKKNLVKTMNGEYIALEKLE 572
Query: 609 EAAMRSSIIQQIVVIG-QDKRRLGAIIVPNSEEVLKVARELSIIDSISS-----NVVSEE 662
+ + I V Q K + IIVPN + K+A++L I++ S N + +
Sbjct: 573 SVYRSNEYVANICVYADQSKTKPVGIIVPNHAPLTKLAKKLGIMEQKDSSINIENYLEDA 632
Query: 663 KVLNLIYKE-LKTWMSESPFQI---GPILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQ 718
K++ +Y + LKT + I I+ + +T NG +T K++R ++ K++
Sbjct: 633 KLIKAVYSDLLKTGKDQGLVGIELLAGIVFFDGEWTPQNGFVTSAQKLKRKDILNAVKDK 692
Query: 719 IDDLY 723
+D +Y
Sbjct: 693 VDAVY 697
>LCF5_MOUSE (Q8JZR0) Long-chain-fatty-acid--CoA ligase 5 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 5) (LACS 5)
Length = 683
Score = 179 bits (453), Expect = 3e-44
Identities = 156/606 (25%), Positives = 267/606 (43%), Gaps = 72/606 (11%)
Query: 133 DEKLALFADNSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEMF 192
D+ + +FA N W++++ + V E ++ + N ++ + D P+
Sbjct: 132 DQFVGIFAQNRPEWVISELACYTYSMVAVPLYDTLGTEAIIFVINRADIPVVICDTPQKA 191
Query: 193 NRIAKPFYS--KTGMRFIILLWGEKSDLNLIAEENKEVPIFSFMEVIDLGRESRMALSDS 250
+ + G++ IIL+ DL + E V + S + ++G+E+
Sbjct: 192 TMLVENVEKGLTPGLKTIILMDPFDDDL-MKRGEKCGVEMLSLHDAENIGKENFKKPVPP 250
Query: 251 HEASQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNLLHQ----IKNLWDTVPAEVG 306
+D++ + +TSGTTG+PKG MLTH N++ +K L
Sbjct: 251 ------------KPEDLSVICFTSGTTGDPKGAMLTHENVVSNMAAFLKFLEPIFQPTSD 298
Query: 307 DRFLSMLPPWHAYERACEYFIFTCG--IEQVYTTVRNLKDDLGRYQPHYMISVPLVFETL 364
D +S LP H +ER + +F+CG I +R L DD+ +P +VP + +
Sbjct: 299 DVTISYLPLAHMFERLVQGILFSCGGKIGFFQGDIRLLPDDMKALKPTVFPTVPRLLNRV 358
Query: 365 YSGIQKQISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWA 424
Y +Q + T P++K + ++LA + + + R D LW
Sbjct: 359 YDKVQNEAKT--PLKKFL------LNLAIISKFNEVKNGIIRR------------DSLWD 398
Query: 425 RIIATILFPIHLLAIKFVYSKIHSAI-GLSKAGISGGGSLPLEVDKFFE-AIGVKVQNGY 482
K V+SKI ++ G + I+G + V FF A+G V Y
Sbjct: 399 ---------------KLVFSKIQGSLGGKVRLMITGAAPISTPVLTFFRAAMGCWVFEAY 443
Query: 483 GLTETSPVIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNG 542
G TE + + P G VG PV K+ D ++G + ++G V G
Sbjct: 444 GQTECTGGCSITSPGDWTAGHVGTPVACNFVKLEDVADMNYFSVNNEGEICIKGNNVFKG 503
Query: 543 YYKNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENV 602
Y K+P T + LDKDGWL+TGD+G P +G + + R K+ L+ GE +
Sbjct: 504 YLKDPEKTQEVLDKDGWLHTGDIGRWLP----------NGTLKIVDRKKNIFKLAQGEYI 553
Query: 603 EPAELEEAAMRSSIIQQIVVIGQDKRR-LGAIIVPNSEEVLKVARELSI---IDSISSNV 658
P ++E RS + Q+ V G+ R L ++VP+ + + A ++ + + + N
Sbjct: 554 APEKIENVYSRSRPVLQVFVHGESLRSFLIGVVVPDPDSLPSFAAKIGVKGSFEELCKNQ 613
Query: 659 VSEEKVLNLIYKELKTWMSESPFQIGPILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQ 718
+E +L + K K +S Q+ I + EPFTI+NGL+TPT+K +R + ++ Q
Sbjct: 614 CVKEAILEDLQKIGKEGGLKSFEQVKSIFVHPEPFTIENGLLTPTLKAKRVELAKFFQTQ 673
Query: 719 IDDLYK 724
I LY+
Sbjct: 674 IKSLYE 679
>LCF2_RAT (P18163) Long-chain-fatty-acid--CoA ligase, liver isozyme
(EC 6.2.1.3) (Long-chain acyl-CoA synthetase 2) (LACS 2)
Length = 699
Score = 177 bits (448), Expect = 1e-43
Identities = 169/637 (26%), Positives = 273/637 (42%), Gaps = 74/637 (11%)
Query: 103 HHPPSTITYNQLEQAILDYAEGLRVIGVRP--DEKLALFADNSCRWLVADQGMMASGAIN 160
+ P I+Y Q+ + L G +P ++ + +F+ N W+ +QG +
Sbjct: 116 NQPYEWISYKQVAEMAECIGSALIQKGFKPCSEQFIGIFSQNRPEWVTIEQGCFTYSMVV 175
Query: 161 VVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYSKTGMRFIILLWGEKSDLNL 220
V + + I N +E + D PE + + +K I++ + D +L
Sbjct: 176 VPLYDTLGTDAITYIVNKAELSVIFADKPEKAKLLLEGVENKLTPCLKIIVIMDSYDNDL 235
Query: 221 IAEENK-EVPIFSFMEVIDLGRESRMALSDSHEASQRYVYEAINSDDIATLIYTSGTTGN 279
+ K V I + DLGR +R +D+A + +TSGTTGN
Sbjct: 236 VERGQKCGVEIIGLKALEDLGRVNRTKPKPPEP------------EDLAIICFTSGTTGN 283
Query: 280 PKGVMLTHRNLLHQ----IKNLWDTVPAEVGDRFLSMLPPWHAYERACEYFIFTCGIEQV 335
PKG M+TH+N+++ IK A D +S LP H +E E + G +
Sbjct: 284 PKGAMVTHQNIMNDCSGFIKATESAFIASPEDVLISFLPLAHMFETVVECVMLCHGAKIG 343
Query: 336 YTT--VRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQISTSPPVRKLVALTFIRVSLAY 393
+ +R L DDL QP VP + ++ I Q +TS R L +
Sbjct: 344 FFQGDIRLLMDDLKVLQPTIFPVVPRLLNRMFDRIFGQANTSVK----------RWLLDF 393
Query: 394 MEYKRIYEGKC-LTRNVKQPSIVNSMLDCLWARIIATILFPIHLLAIKFVYSKIHSAIGL 452
++ E + + RN NS+ D K ++ KI S++G
Sbjct: 394 ASKRKEAELRSGIVRN-------NSLWD-------------------KLIFHKIQSSLGG 427
Query: 453 S-KAGISGGGSLPLEVDKFFEA-IGVKVQNGYGLTETSPVIAARRPRCNVIGSVGHPVQH 510
+ I+G + V F A +G + GYG TE + P G VG P+
Sbjct: 428 KVRLMITGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLSLPGDWTAGHVGAPMPC 487
Query: 511 TEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQALDKDGWLNTGDLGWIAP 570
K+VD E +G + V+G V GY K+P T +ALDKDGWL+TGD+G P
Sbjct: 488 NYIKLVDVEDMNYQAAKGEGEVCVKGANVFKGYLKDPARTAEALDKDGWLHTGDIGKWLP 547
Query: 571 HHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRSSIIQQIVVIGQDKRR- 629
+G + + R K L+ GE + P ++E +RS + Q+ V G+ +
Sbjct: 548 ----------NGTLKIIDRKKHIFKLAQGEYIAPEKIENIYLRSEAVAQVFVHGESLQAF 597
Query: 630 LGAIIVPNSEEVLKVARELSI---IDSISSNVVSEEKVLNLIYKELKTWMSESPFQIGPI 686
L AI+VP+ E + A++ + + N + +L + K K + Q+ I
Sbjct: 598 LIAIVVPDVEILPSWAQKRGFQGSFEELCRNKDINKAILEDMVKLGKNAGLKPFEQVKGI 657
Query: 687 LLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQIDDLY 723
+ E F+IDNGL+TPT+K +R + ++ QID+LY
Sbjct: 658 AVHPELFSIDNGLLTPTLKAKRPELRNYFRSQIDELY 694
>LCF6_HUMAN (Q9UKU0) Long-chain-fatty-acid--CoA ligase 6 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 6) (LACS 6)
Length = 697
Score = 163 bits (412), Expect = 2e-39
Identities = 157/608 (25%), Positives = 260/608 (41%), Gaps = 78/608 (12%)
Query: 133 DEKLALFADNSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEMF 192
D+ + +FA N W++ + + V + I N ++ + VD P+
Sbjct: 147 DQFIGVFAQNRPEWIIVELACYTYSMVVVPLYDTLGPGAIRYIINTADISTVIVDKPQKA 206
Query: 193 NRIAKPFYSKT--GMRFIILLWGEKSDLNLIAEENKEVPIFSFMEVIDLGRESRMALSDS 250
+ + K G++ IIL+ + L ++ V I S V D G+E+ A
Sbjct: 207 VLLLEHVERKETPGLKLIILMDPFEEALKERGQKCGVV-IKSMQAVEDCGQENHQAPVPP 265
Query: 251 HEASQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDTVPAE----VG 306
DD++ + +TSGTTGNPKG MLTH N++ ++
Sbjct: 266 QP------------DDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTESQWAPTCA 313
Query: 307 DRFLSMLPPWHAYERACEYFIFTCG--IEQVYTTVRNLKDDLGRYQPHYMISVPLVFETL 364
D +S LP H +ER + ++ G + +R L DD+ P VP + +
Sbjct: 314 DVHISYLPLAHMFERMVQSVVYCHGGRVGFFQGDIRLLSDDMKALCPTIFPVVPRLLNRM 373
Query: 365 YSGIQKQISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSML--DCL 422
Y I Q +T P R L+ R KQ + + ++ D +
Sbjct: 374 YDKIFSQANT-PLKRWLLEFAAKR---------------------KQAEVRSGIIRNDSI 411
Query: 423 WARIIATILFPIHLLAIKFVYSKIHSAIGLSKAGISGGGS--LPLEVDKFFEAIGVKVQN 480
W + ++KI +++G I G + P + A+G +V
Sbjct: 412 WDELF---------------FNKIQASLGGCVRMIVTGAAPASPTVLGFLRAALGCQVYE 456
Query: 481 GYGLTETSPVIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVM 540
GYG TE + P G VG P+ K+VD E +G + VRGP V
Sbjct: 457 GYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDVEELNYWACKGEGEICVRGPNVF 516
Query: 541 NGYYKNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGE 600
GY K+P T +ALD DGWL+TGD+G P +G + + R K L+ GE
Sbjct: 517 KGYLKDPDRTKEALDSDGWLHTGDIGKWLP----------AGTLKIIDRKKHIFKLAQGE 566
Query: 601 NVEPAELEEAAMRSSIIQQIVVIGQD-KRRLGAIIVPNSEEVLKVARELSIIDSISSNVV 659
V P ++E +RS + QI V G K L I+VP+ E + A++ I+ +++
Sbjct: 567 YVAPEKIENIYIRSQPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKRG-IEGTYADLC 625
Query: 660 SEEKVLNLIYKELKTWMSESPF----QIGPILLVNEPFTIDNGLMTPTMKIRRDRVVAKY 715
+ + + I +++ ES Q+ I + ++ F++ NGL+TPT+K +R + +
Sbjct: 626 TNKDLKKAILEDMVRLGKESGLHSFEQVKAIHIHSDMFSVQNGLLTPTLKAKRPELREYF 685
Query: 716 KEQIDDLY 723
K+QI++LY
Sbjct: 686 KKQIEELY 693
>FD11_MYCTU (Q10776) Putative fatty-acid--CoA ligase fadD11 (EC
6.2.1.-) (Acyl-CoA synthetase)
Length = 571
Score = 163 bits (412), Expect = 2e-39
Identities = 139/477 (29%), Positives = 226/477 (47%), Gaps = 62/477 (12%)
Query: 259 YEAINSDDIATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDTVPAEVGDRFLSMLPPWHA 318
+ A+ +DI TLIYTSGTTGNPKGV +TH NLL + + + + GDR S LP H
Sbjct: 125 WRAVQPEDIVTLIYTSGTTGNPKGVEMTHANLLFEGYAIDEVLGIRFGDRVTSFLPSAHI 184
Query: 319 YERACEYFIFTCGIEQV--YTTVRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQISTSP 376
+R ++ QV R + L +P +VP V+E L +GI+ ++
Sbjct: 185 ADRMTGLYLQEMFGTQVTAVADARTIAAALPDVRPTVWGAVPRVWEKLKAGIEFTVARET 244
Query: 377 PVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWARIIATILFPIHL 436
K AL + +S+A + G+ + + L WA+
Sbjct: 245 DEMKRQALAW-AMSVAGKRANALLAGESM----------SDQLVAEWAK----------- 282
Query: 437 LAIKFVYSKIHSAIGLSKA--GISGGGSLPLEVDKFFEAIGVKVQNGYGLTETSPVIAAR 494
A + V SK+ +G + +SG +P E FF IG+ + +G++E S V A
Sbjct: 283 -ADELVLSKLRERLGFGELRWALSGAAPIPKETLAFFAGIGIPIAEIWGMSELSCVATAS 341
Query: 495 RPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQAL 554
PR +G+VG + + K+ +E GE L VRGP VM GY K P T +A+
Sbjct: 342 HPRDGRLGTVGKLLPGLQGKI--AEDGEYL---------VRGPLVMKGYRKEPAKTAEAI 390
Query: 555 DKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRS 614
D DGWL+TGD+ I +S G + V R K+ I+ + G+N+ PA +E + +
Sbjct: 391 DSDGWLHTGDVFDI----------DSDGYLRVVDRKKELIINAAGKNMSPANIENTILAA 440
Query: 615 -SIIQQIVVIGQDKRRLGAIIVPNSEEVLKVARELSIIDSISSNVVSEEKVLNLI----- 668
++ ++ IG + A++V +++ + A + +D+ + + ++ +V+ I
Sbjct: 441 CPMVGVMMAIGDGRTYNTALLVFDADSLGPYAAQRG-LDASPAALAADPEVIARIAAGVA 499
Query: 669 --YKELKTWMSESPFQIGPILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKEQIDDLY 723
+L F+I P L EP +T TMK++R R+ AKY +I++LY
Sbjct: 500 EGNAKLSRVEQIKRFRILPTLW--EP---GGDEITLTMKLKRRRIAAKYSAEIEELY 551
>LCF6_MOUSE (Q91WC3) Long-chain-fatty-acid--CoA ligase 6 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 6) (LACS 6)
Length = 697
Score = 161 bits (407), Expect = 6e-39
Identities = 161/611 (26%), Positives = 259/611 (42%), Gaps = 78/611 (12%)
Query: 130 VRPDEKLALFADNSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGP 189
V ++ + +FA N W++A+ + V + I N ++ + VD P
Sbjct: 144 VGTEQFVGVFAQNRPEWIIAELACYTYSMVVVPLYDTLGPGSISYIINTADICTVIVDKP 203
Query: 190 EMFNRIAKPFYSKT--GMRFIILLWGEKSDLNLIAEENKE--VPIFSFMEVIDLGRESRM 245
+ + K G++ +IL+ + L E K+ V I S + D GRE+
Sbjct: 204 HKATLLLEHVERKETPGLKLVILMEPFEDALR---ERGKKCGVDIKSMQAIEDCGRENHH 260
Query: 246 ALSDSHEASQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDTVPAEV 305
A DD++ + +TSGTTGNPKG MLTH N++ +
Sbjct: 261 APVPPRP------------DDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTEKVI 308
Query: 306 ----GDRFLSMLPPWHAYERACEYFIFTCG--IEQVYTTVRNLKDDLGRYQPHYMISVPL 359
D +S LP H +ER + ++ G + +R L DD+ +P VP
Sbjct: 309 FPRQDDVLISFLPLAHMFERVIQSVVYCHGGRVGFFQGDIRLLSDDMKALRPTIFPVVPR 368
Query: 360 VFETLYSGIQKQISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSML 419
+ +Y I Q TS K L F A + + G + RN NS+
Sbjct: 369 LLNRMYDKIFHQADTS---LKRWLLEFA----AKRKQAEVRSG--IIRN-------NSIW 412
Query: 420 DCLWARIIATILFPIHLLAIKFVYSKIHSAIGLSKAGISGGGS--LPLEVDKFFEAIGVK 477
D L+ ++KI +++G I G + P + A+G +
Sbjct: 413 DELF-------------------FNKIQASLGGHVRMIVTGAAPASPTVLGFLRAALGCQ 453
Query: 478 VQNGYGLTETSPVIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGP 537
V GYG TE + P G VG P+ K+VD+E +G + V+GP
Sbjct: 454 VYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDAEELNYWTCKGEGEICVKGP 513
Query: 538 PVMNGYYKNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLS 597
V GY K+ T +ALD DGWL+TGD+G P G + + R K L+
Sbjct: 514 NVFKGYLKDEDRTKEALDSDGWLHTGDIGKWLP----------EGTLKIIDRKKHIFKLA 563
Query: 598 TGENVEPAELEEAAMRSSIIQQIVVIGQD-KRRLGAIIVPNSEEVLKVARELSIIDSISS 656
GE V P ++E +RS + QI V G K L I+VP+ E + A++ I+
Sbjct: 564 QGEYVAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPDPEVMPSWAQKKG-IEGTYQ 622
Query: 657 NVVSEEKVLNLIYKELKTWMSESPF----QIGPILLVNEPFTIDNGLMTPTMKIRRDRVV 712
+ ++++ I ++ ES Q+ I + + F++ NGL+TPT+K +R +
Sbjct: 623 ELCMKKELKKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLKAKRPELR 682
Query: 713 AKYKEQIDDLY 723
+K+QI++LY
Sbjct: 683 EYFKKQIEELY 693
>LCF6_RAT (P33124) Long-chain-fatty-acid--CoA ligase 6 (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase 6) (LACS 6)
(Long-chain-fatty-acid--CoA ligase, brain isozyme)
Length = 697
Score = 159 bits (402), Expect = 2e-38
Identities = 161/609 (26%), Positives = 262/609 (42%), Gaps = 74/609 (12%)
Query: 130 VRPDEKLALFADNSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGP 189
V ++ + +FA N W++A+ + V + I N ++ + VD P
Sbjct: 144 VGTEQFIGVFAQNRPEWIIAELACYTYSMVVVPLYDTLGPGSIRYIINTADICTVIVDKP 203
Query: 190 EMFNRIAKPFYSKT--GMRFIILLWGEKSDLNLIAEENKEVPIFSFMEVIDLGRESRMAL 247
+ + K G++ +IL+ E D + + E K+ + D+ +S A+
Sbjct: 204 HKAILLLEHVERKETPGLKLVILM--EPFD-DALRERGKKCGV-------DI--KSMQAI 251
Query: 248 SDSHEASQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDTVPAE--- 304
DS + + R V DD++ + +TSGTTGNPKG MLTH N++ ++
Sbjct: 252 EDSGQENHR-VPVPPRPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTESQWAP 310
Query: 305 -VGDRFLSMLPPWHAYERACEYFIFTCG--IEQVYTTVRNLKDDLGRYQPHYMISVPLVF 361
D S LP H +ER + ++ G + +R L DD+ +P VP +
Sbjct: 311 TCADVHFSYLPLAHMFERMVQSVVYCHGGRVGFFQGDIRLLSDDMKALRPTIFPVVPRLL 370
Query: 362 ETLYSGIQKQISTSPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSMLDC 421
+Y I Q TS K L F A + + G + RN NS+ D
Sbjct: 371 NRMYDKIFHQADTS---LKRWLLEFA----AKRKQAEVRSG--IIRN-------NSIWDE 414
Query: 422 LWARIIATILFPIHLLAIKFVYSKIHSAIGLSKAGISGGGS--LPLEVDKFFEAIGVKVQ 479
L+ ++KI +++G I G + P + A+G +V
Sbjct: 415 LF-------------------FNKIQASLGGHVRMIVTGAAPASPTVLGFLRAALGCQVY 455
Query: 480 NGYGLTETSPVIAARRPRCNVIGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPV 539
GYG TE + P G VG P+ K+VD+E +G + V+GP V
Sbjct: 456 EGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDAEELNYWTSKGEGEICVKGPNV 515
Query: 540 MNGYYKNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTG 599
GY K+ T +ALD DGWL+TGD+G P G + + R K L+ G
Sbjct: 516 FKGYLKDEDRTKEALDSDGWLHTGDIGKWLP----------EGTLKIIDRKKHIFKLAQG 565
Query: 600 ENVEPAELEEAAMRSSIIQQIVVIGQD-KRRLGAIIVPNSEEVLKVARELSIIDSISSNV 658
E V P ++E +RS + QI V G K L I+VP+ EV+ + I+ +
Sbjct: 566 EYVAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPD-PEVMPCWAQKKGIEGNYQEL 624
Query: 659 VSEEKVLNLIYKELKTWMSESPF----QIGPILLVNEPFTIDNGLMTPTMKIRRDRVVAK 714
+++ I ++ ES Q+ I + + F++ NGL+TPT+K +R +
Sbjct: 625 CKSKELKKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLKAKRPELREY 684
Query: 715 YKEQIDDLY 723
+K+QI++LY
Sbjct: 685 FKKQIEELY 693
>LCF2_YEAST (P39518) Long-chain-fatty-acid--CoA ligase 2 (EC
6.2.1.3) (Long-chain acyl-CoA synthetase 2) (Fatty acid
activator 2)
Length = 744
Score = 134 bits (337), Expect = 8e-31
Identities = 132/486 (27%), Positives = 217/486 (44%), Gaps = 71/486 (14%)
Query: 265 DDIATLIYTSGTTGNPKGVMLTHRNLLHQIKNLWDT--VPAEVGDRFL---SMLPPWHAY 319
D + T+ +TSGTTG PKGV ++HRN+ I + T +P + ++ L LP H +
Sbjct: 292 DSLYTISFTSGTTGLPKGVEMSHRNIASGIAFAFSTFRIPPDKRNQQLYDMCFLPLAHIF 351
Query: 320 ERACEYFIFTCG-----IEQVYTTVRNLKDDLGRYQPHYMISVPLVFETLYSGIQKQIST 374
ER + G + + TV L +DL +P+ + VP + +GI+ +
Sbjct: 352 ERMVIAYDLAIGFGIGFLHKPDPTV--LVEDLKILKPYAVALVPRILTRFEAGIKNALDK 409
Query: 375 SPPVRKLVALTFIRVSLAYMEYKRIYEGKCLTRNVKQPSIVNSMLDCLWARIIATILFPI 434
S V++ VA T + A + R SI+N
Sbjct: 410 ST-VQRNVANTILDSKSA----------RFTARGGPDKSIMN------------------ 440
Query: 435 HLLAIKFVYSKIHSAIGLSKAGISGGGSLPLEVDKFF---EAIGVKVQNGYGLTETSPVI 491
L+ + + KI ++GLS GS P+ D A+ + ++ GYGLTET +
Sbjct: 441 FLVYHRVLIDKIRDSLGLSNNSFIITGSAPISKDTLLFLRSALDIGIRQGYGLTETFAGV 500
Query: 492 AARRPRCNVIGSVGHPVQHTEFKVVD-SETGEVLPPGSKGILKVRGPPVMNGYYKNPLAT 550
P +GS G E ++ E G KG L++RGP V Y+KNP T
Sbjct: 501 CLSEPFEKDVGSCGAIGISAECRLKSVPEMGYHADKDLKGELQIRGPQVFERYFKNPNET 560
Query: 551 NQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEA 610
++A+D+DGW +TGD+ +I + G I V R K+ L+ GE + P ++E
Sbjct: 561 SKAVDQDGWFSTGDVAFI----------DGKGRISVIDRVKNFFKLAHGEYIAPEKIENI 610
Query: 611 AMRS-SIIQQIVV------------IGQDKRRLGAIIVPNSEEVLKVARELSIIDSISSN 657
+ S I QI V +G D I+ EV +E+ ++++++ N
Sbjct: 611 YLSSCPYITQIFVFGDPLKTFLVGIVGVDVDAAQPILAAKHPEVKTWTKEV-LVENLNRN 669
Query: 658 VVSEEKVLNLIYKELKTWMSESPFQIGPILLVNEPFTIDNGLMTPTMKIRRDRVVAKYKE 717
++ LN I K T + ++ I + EP T+++ ++TPT KI+R + +K+
Sbjct: 670 KKLRKEFLNKINK--CTDGLQGFEKLHNIKVGLEPLTLEDDVVTPTFKIKRAKASKFFKD 727
Query: 718 QIDDLY 723
+D LY
Sbjct: 728 TLDQLY 733
>LCFA_BACSU (P94547) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase)
Length = 560
Score = 106 bits (265), Expect = 2e-22
Identities = 69/180 (38%), Positives = 97/180 (53%), Gaps = 14/180 (7%)
Query: 447 HSAIGLSKAGISGGGSLPLEVDKFFEAI-GVKVQNGYGLTETSPVIAARRP-RCNVIGSV 504
H + K+ +SG +LP+EV + FE + G K+ GYGL+E SPV A N GS+
Sbjct: 319 HYDLSSIKSCLSGSAALPVEVKQKFEKVTGGKLVEGYGLSEASPVTHANFIWGKNKPGSI 378
Query: 505 GHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYYKNPLATNQALDKDGWLNTGD 564
G P T+ + ETGE+ P G + V+GP VM GY+ P T L +DGWL TGD
Sbjct: 379 GCPWPSTDAAIYSEETGELAAPYEHGEIIVKGPQVMKGYWNKPEETAAVL-RDGWLFTGD 437
Query: 565 LGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEPAELEEAAMRSSIIQQIVVIG 624
+G++ + G + R KD I+++ G N+ P E+EEA IQ+IVV G
Sbjct: 438 MGYM----------DEEGFFYIADRKKD-IIIAGGYNIYPREVEEALYEHEAIQEIVVAG 486
Score = 55.1 bits (131), Expect = 7e-07
Identities = 62/248 (25%), Positives = 113/248 (45%), Gaps = 16/248 (6%)
Query: 79 KVVPDIWRSSAEKYGDKVALVDQYHHPPSTITYNQLEQAILDYAEGLRVIGVRPDEKLAL 138
K + I SA ++ DK A+ +T++ + L A L+ G++ +++A+
Sbjct: 24 KTLQSILTDSAARFPDKTAI----SFYGKKLTFHDILTDALKLAAFLQCNGLQKGDRVAV 79
Query: 139 FADNSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKP 198
N + +++ G++ +G I VV+ + E L+ V++ + +F + K
Sbjct: 80 MLPNCPQTVISYYGVLFAGGI-VVQTNPLYTEHELEYQLRDAQVSVIITLDLLFPKAIK- 137
Query: 199 FYSKTGMRFIILLWGEKSDLNLIAEENKEVPIFSFMEV-IDLGRESRM---ALSDSHEAS 254
+ IL+ K L +N P+ +V ID + + + A E +
Sbjct: 138 -MKTLSIVDQILITSVKDYLPF--PKNILYPLTQKQKVHIDFDKTANIHTFASCMKQEKT 194
Query: 255 QRYVYEAINSD-DIATLIYTSGTTGNPKGVMLTHRNLL--HQIKNLWDTVPAEVGDRFLS 311
+ I+ + DIA L YT GTTG PKGVMLTH+N+L ++ W E ++ L
Sbjct: 195 ELLTIPKIDPEHDIAVLQYTGGTTGAPKGVMLTHQNILANTEMCAAWMYDVKEGAEKVLG 254
Query: 312 MLPPWHAY 319
++P +H Y
Sbjct: 255 IVPFFHVY 262
>LCFA_SALTY (P63521) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase)
Length = 561
Score = 103 bits (257), Expect = 2e-21
Identities = 66/200 (33%), Positives = 110/200 (55%), Gaps = 17/200 (8%)
Query: 429 TILFPIHLLAIKFVYSKIHSAIGLSKAGISGGGSLPLE---VDKFFEAIGVKVQNGYGLT 485
T + ++ L + +K + S +S GG +P++ +++ + G + GYGLT
Sbjct: 301 TAMTGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQNVVAERWVKLTGQYLLEGYGLT 360
Query: 486 ETSPVIAARRPRCNV-IGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYY 544
E +P+++ + GS+G PV TE K+VD + EV P G G L V+GP VM GY+
Sbjct: 361 ECAPLVSVNPHDIDYHSGSIGLPVPSTEAKLVDDDDNEVAP-GEAGELCVKGPQVMLGYW 419
Query: 545 KNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEP 604
+ P AT++ + KDGWL+TGD+ + + G + + R KD I++S G NV P
Sbjct: 420 QRPDATDEII-KDGWLHTGDIAVM----------DEDGFLRIVDRKKDMILVS-GFNVYP 467
Query: 605 AELEEAAMRSSIIQQIVVIG 624
E+E+ M+ S +Q++ +G
Sbjct: 468 NEIEDVVMQHSGVQEVAAVG 487
Score = 74.3 bits (181), Expect = 1e-12
Identities = 84/339 (24%), Positives = 151/339 (43%), Gaps = 39/339 (11%)
Query: 83 DIWRSSAEKYGDKVALVDQYHHPPSTITYNQLEQAILDYAEGLRV-IGVRPDEKLALFAD 141
+++ +A +Y D+ A V+ +T+ +LE+ +A L+ +G++ +++AL
Sbjct: 27 ELFEHAATRYADQPAFVNM----GEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMP 82
Query: 142 NSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYS 201
N ++ VA G++ +G I V + EL N S + A+ + F +
Sbjct: 83 NLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAAAIII--VSNFAHTLEKVVE 140
Query: 202 KTGMRFIILL-------WGEKSDLNLIAEENKE-VPIFSFMEVIDLGRESRMALSDSHEA 253
KT ++ +IL + + +N + + K VP + + I R AL H
Sbjct: 141 KTSVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHLPDAISF----RSALQ--HGY 194
Query: 254 SQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNL---LHQIKNLWDTVPAEVGDRFL 310
+YV + ++D+A L YT GTTG KG MLTHRN+ L Q+K + + + +
Sbjct: 195 RMQYVKPEVVAEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVKATYGPLLHPGKELVV 254
Query: 311 SMLPPWH--AYERACEYFIFTCGIEQVYTTVRN---LKDDLGRYQPHYMISVPLVFETL- 364
+ LP +H A C FI G + T R+ L +L +Y M V +F L
Sbjct: 255 TALPLYHIFALTMNCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAMTGVNTLFNALL 314
Query: 365 ---------YSGIQKQISTSPPVRKLVALTFIRVSLAYM 394
+S + PV+ +VA +++++ Y+
Sbjct: 315 NNKEFQQLDFSSLHLSAGGGMPVQNVVAERWVKLTGQYL 353
>LCFA_SALTI (P63522) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase)
Length = 561
Score = 103 bits (257), Expect = 2e-21
Identities = 66/200 (33%), Positives = 110/200 (55%), Gaps = 17/200 (8%)
Query: 429 TILFPIHLLAIKFVYSKIHSAIGLSKAGISGGGSLPLE---VDKFFEAIGVKVQNGYGLT 485
T + ++ L + +K + S +S GG +P++ +++ + G + GYGLT
Sbjct: 301 TAMTGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQNVVAERWVKLTGQYLLEGYGLT 360
Query: 486 ETSPVIAARRPRCNV-IGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYY 544
E +P+++ + GS+G PV TE K+VD + EV P G G L V+GP VM GY+
Sbjct: 361 ECAPLVSVNPHDIDYHSGSIGLPVPSTEAKLVDDDDNEVAP-GEAGELCVKGPQVMLGYW 419
Query: 545 KNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEP 604
+ P AT++ + KDGWL+TGD+ + + G + + R KD I++S G NV P
Sbjct: 420 QRPDATDEII-KDGWLHTGDIAVM----------DEDGFLRIVDRKKDMILVS-GFNVYP 467
Query: 605 AELEEAAMRSSIIQQIVVIG 624
E+E+ M+ S +Q++ +G
Sbjct: 468 NEIEDVVMQHSGVQEVAAVG 487
Score = 74.3 bits (181), Expect = 1e-12
Identities = 84/339 (24%), Positives = 151/339 (43%), Gaps = 39/339 (11%)
Query: 83 DIWRSSAEKYGDKVALVDQYHHPPSTITYNQLEQAILDYAEGLRV-IGVRPDEKLALFAD 141
+++ +A +Y D+ A V+ +T+ +LE+ +A L+ +G++ +++AL
Sbjct: 27 ELFEHAATRYADQPAFVNM----GEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMP 82
Query: 142 NSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYS 201
N ++ VA G++ +G I V + EL N S + A+ + F +
Sbjct: 83 NLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGAAAIII--VSNFAHTLEKVVE 140
Query: 202 KTGMRFIILL-------WGEKSDLNLIAEENKE-VPIFSFMEVIDLGRESRMALSDSHEA 253
KT ++ +IL + + +N + + K VP + + I R AL H
Sbjct: 141 KTSVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHLPDAISF----RSALQ--HGY 194
Query: 254 SQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNL---LHQIKNLWDTVPAEVGDRFL 310
+YV + ++D+A L YT GTTG KG MLTHRN+ L Q+K + + + +
Sbjct: 195 RMQYVKPEVVAEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVKATYGPLLHPGKELVV 254
Query: 311 SMLPPWH--AYERACEYFIFTCGIEQVYTTVRN---LKDDLGRYQPHYMISVPLVFETL- 364
+ LP +H A C FI G + T R+ L +L +Y M V +F L
Sbjct: 255 TALPLYHIFALTMNCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAMTGVNTLFNALL 314
Query: 365 ---------YSGIQKQISTSPPVRKLVALTFIRVSLAYM 394
+S + PV+ +VA +++++ Y+
Sbjct: 315 NNKEFQQLDFSSLHLSAGGGMPVQNVVAERWVKLTGQYL 353
>LCFA_ECOLI (P29212) Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3)
(Long-chain acyl-CoA synthetase)
Length = 561
Score = 102 bits (254), Expect = 4e-21
Identities = 65/200 (32%), Positives = 110/200 (54%), Gaps = 17/200 (8%)
Query: 429 TILFPIHLLAIKFVYSKIHSAIGLSKAGISGGGSLPLE---VDKFFEAIGVKVQNGYGLT 485
T + ++ L + +K + S +S GG +P++ +++ + G + GYGLT
Sbjct: 301 TAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTGQYLLEGYGLT 360
Query: 486 ETSPVIAARRPRCNV-IGSVGHPVQHTEFKVVDSETGEVLPPGSKGILKVRGPPVMNGYY 544
E +P+++ + GS+G PV TE K+VD + EV PPG G L V+GP VM GY+
Sbjct: 361 ECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLVDDDDNEV-PPGQPGELCVKGPQVMLGYW 419
Query: 545 KNPLATNQALDKDGWLNTGDLGWIAPHHSTGRSRNSSGVIVVDGRAKDTIVLSTGENVEP 604
+ P AT++ + K+GWL+TGD+ + + G + + R KD I++S G NV P
Sbjct: 420 QRPDATDEII-KNGWLHTGDIAVM----------DEEGFLRIVDRKKDMILVS-GFNVYP 467
Query: 605 AELEEAAMRSSIIQQIVVIG 624
E+E+ M+ +Q++ +G
Sbjct: 468 NEIEDVVMQHPGVQEVAAVG 487
Score = 70.5 bits (171), Expect = 1e-11
Identities = 81/339 (23%), Positives = 150/339 (43%), Gaps = 39/339 (11%)
Query: 83 DIWRSSAEKYGDKVALVDQYHHPPSTITYNQLEQAILDYAEGLRV-IGVRPDEKLALFAD 141
D++ S +Y D+ A V+ +T+ +LE+ +A L+ +G++ +++AL
Sbjct: 27 DMFEQSVARYADQPAFVNM----GEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMP 82
Query: 142 NSCRWLVADQGMMASGAINVVRGSRSSVEELLQIYNHSESVALAVDGPEMFNRIAKPFYS 201
N ++ VA G++ +G I V + EL N S + A+ + F +
Sbjct: 83 NLLQYPVALFGILRAGMIVVNVNPLYTPRELEHQLNDSGASAIVI--VSNFAHTLEKVVD 140
Query: 202 KTGMRFIILL-------WGEKSDLNLIAEENKE-VPIFSFMEVIDLGRESRMALSDSHEA 253
KT ++ +IL + + +N + + K VP + + I R AL + +
Sbjct: 141 KTAVQHVILTRMGDQLSTAKGTVVNFVVKYIKRLVPKYHLPDAISF----RSALHNGYR- 195
Query: 254 SQRYVYEAINSDDIATLIYTSGTTGNPKGVMLTHRNL---LHQIKNLWDTVPAEVGDRFL 310
+YV + +D+A L YT GTTG KG MLTHRN+ L Q+ + + + +
Sbjct: 196 -MQYVKPELVPEDLAFLQYTGGTTGVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVV 254
Query: 311 SMLPPWHAYERA--CEYFIFTCGIEQVYTTVRN---LKDDLGRYQPHYMISVPLVFETL- 364
+ LP +H + C FI G + T R+ L +L +Y + V +F L
Sbjct: 255 TALPLYHIFALTINCLLFIELGGQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALL 314
Query: 365 ---------YSGIQKQISTSPPVRKLVALTFIRVSLAYM 394
+S + PV+++VA +++++ Y+
Sbjct: 315 NNKEFQQLDFSSLHLSAGGGMPVQQVVAERWVKLTGQYL 353
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.136 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,520,551
Number of Sequences: 164201
Number of extensions: 3604053
Number of successful extensions: 11058
Number of sequences better than 10.0: 222
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 29
Number of HSP's that attempted gapping in prelim test: 10279
Number of HSP's gapped (non-prelim): 630
length of query: 724
length of database: 59,974,054
effective HSP length: 118
effective length of query: 606
effective length of database: 40,598,336
effective search space: 24602591616
effective search space used: 24602591616
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 70 (31.6 bits)
Medicago: description of AC146585.9