BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146568.1 + phase: 0
(610 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BIOC_ERWHE (O06898) Biotin synthesis protein bioC 37 0.15
Y143_SYNY3 (P74442) Hypothetical WD-repeat protein slr0143 35 0.57
ZAN_MOUSE (O88799) Zonadhesin precursor 34 0.98
YT37_STRFR (P20187) Hypothetical 37.1 kDa protein in transposon ... 34 0.98
ALF_SCHPO (P36580) Fructose-bisphosphate aldolase (EC 4.1.2.13) 33 1.7
ADIP_MOUSE (Q8VC66) Afadin- and alpha-actinin-binding protein (A... 33 2.2
YJJK_HAEIN (P45127) ABC transporter ATP-binding protein HI1252 33 2.8
GUNB_NEOPA (Q12647) Endoglucanase B precursor (EC 3.2.1.4) (Endo... 33 2.8
ADIP_RAT (Q8CGZ2) Afadin- and alpha-actinin-binding protein (ADI... 33 2.8
CHD2_HUMAN (O14647) Chromodomain-helicase-DNA-binding protein 2 ... 32 3.7
YJJK_ECOLI (P37797) ABC transporter ATP-binding protein yjjK 32 4.8
BIOC_ECOLI (P12999) Biotin synthesis protein bioC 32 4.8
SYH_YEAST (P07263) Histidyl-tRNA synthetase, mitochondrial precu... 32 6.3
CTB6_HUMAN (Q96HY6) Protein C20orf116 precursor 32 6.3
AL13_SCHPO (O13953) Cell polarity protein alp13 (Altered polarit... 32 6.3
TONB_HAEDU (O51810) TonB protein 31 8.3
INVO_CANFA (P18174) Involucrin 31 8.3
>BIOC_ERWHE (O06898) Biotin synthesis protein bioC
Length = 251
Score = 37.0 bits (84), Expect = 0.15
Identities = 31/97 (31%), Positives = 42/97 (42%), Gaps = 10/97 (10%)
Query: 208 TALDTGCGVASWGAYLW---SRNVVAMSFAPRDSHEAQVQFALERGVPAVIGVFGTIKLP 264
+ LD GCG S Y W V A+ + +AQ Q A + V A I LP
Sbjct: 45 SVLDAGCGPGSMSRY-WRDAGAEVTALDLSLPMLRQAQSQQAAQHYVAADIEA-----LP 98
Query: 265 YPSRAFDMAHCSRCLIPWGANDGMYMMEVDRVLRPGG 301
FD+A S + W + G + + RV+RPGG
Sbjct: 99 LADARFDLAW-SNLAVQWCNDLGQALKSLHRVVRPGG 134
>Y143_SYNY3 (P74442) Hypothetical WD-repeat protein slr0143
Length = 1191
Score = 35.0 bits (79), Expect = 0.57
Identities = 27/106 (25%), Positives = 44/106 (41%), Gaps = 7/106 (6%)
Query: 307 GPPINWKVNYKPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQKMTDTESCRSR 366
G P+ KV KPW R +E+L Q K+ E + + + ++ + ++ S
Sbjct: 102 GLPLAGKVALKPWLREREQLSPIQ-KLREFVETIILQTIGDRQIVIFIDEIDKVLSLPFS 160
Query: 367 QDD--SSVEFCESSDPDDVWYKKLK----ACVTPTPKVSGGDLKPF 406
DD S + FC + DD Y +L TP+ + PF
Sbjct: 161 LDDFFSYIRFCYNQRADDHEYNRLSFALFGVATPSDLIDNKTQTPF 206
>ZAN_MOUSE (O88799) Zonadhesin precursor
Length = 5376
Score = 34.3 bits (77), Expect = 0.98
Identities = 24/73 (32%), Positives = 31/73 (41%), Gaps = 9/73 (12%)
Query: 375 CESSDPDDVWYKKLKACVTPTPKVSGGDLKPFPDRLYAIPPRVSSGSIPGVSSETYQNDN 434
C SD DDVW KK + P L PF + A+PP+ S S ET DN
Sbjct: 1858 CADSDMDDVWTKKCAILMNP--------LGPFSNCHEAVPPQASFSSCVYGQCET-NGDN 1908
Query: 435 KMWKKHVNAYKKI 447
+ + AY +
Sbjct: 1909 LTFCHSLQAYASL 1921
>YT37_STRFR (P20187) Hypothetical 37.1 kDa protein in transposon
TN4556
Length = 345
Score = 34.3 bits (77), Expect = 0.98
Identities = 47/180 (26%), Positives = 70/180 (38%), Gaps = 33/180 (18%)
Query: 149 PYANAPYKSLTVEKAIQNWIQYEGNVFRFPGGGTQFPQGADKYIDQLAS----------- 197
P+ P +E+ + + G VF + P Y+DQLA+
Sbjct: 65 PFCLTPAGRELLEREAHSMARLSGRVFE-----SAVPDEVS-YLDQLATTDAARSYKSVM 118
Query: 198 VIPINDGTVRTALDTGCGVASWGAYLWSRNVVAMSFAPR------DSHEAQVQFALER-- 249
+ ++ +ALD GCG + +A + +P DS + V+ A R
Sbjct: 119 LDALDARPGESALDLGCGPGT------DLGTLAKAVSPSGRVIGIDSSQEMVEQARRRTE 172
Query: 250 GVPAVIGVFGTIK-LPYPSRAFDMAHCSRCLIPWGANDGMYMMEVDRVLRPGGYWVLSGP 308
+PAV G I LP + D A R L A+ + E RVLRPGG V+ P
Sbjct: 173 NLPAVEVELGDIHTLPLEDGSIDCARTDRVL-QHVADPAQALAEARRVLRPGGRLVMGEP 231
>ALF_SCHPO (P36580) Fructose-bisphosphate aldolase (EC 4.1.2.13)
Length = 358
Score = 33.5 bits (75), Expect = 1.7
Identities = 35/124 (28%), Positives = 60/124 (48%), Gaps = 22/124 (17%)
Query: 274 HCSRCLIPWGANDGMYMMEVDRVLRPGGYWVLSGPPINWKVNYKPWQRPKEELEEEQRKI 333
HC++ L+PW DGM+ E D Y+ + G P+ + +L EE +K
Sbjct: 109 HCAKKLLPW--MDGMF--EADE-----AYFKIHGEPL--------FSSHMLDLSEEPKK- 150
Query: 334 EEVAKKLCWEKKSEKAEIAIWQKMTDTESCRSRQDDSSVEFCE-SSDPDDVW--YKKLKA 390
E +A+ + K++ +I I ++ T D+S V E + P+D+W Y++L +
Sbjct: 151 ENIAQVKEYCKRAVPMKIWIEMEIGITGGEEDGVDNSHVSHTELYTQPEDIWDVYRELSS 210
Query: 391 CVTP 394
VTP
Sbjct: 211 -VTP 213
>ADIP_MOUSE (Q8VC66) Afadin- and alpha-actinin-binding protein
(ADIP) (Afadin DIL domain-interacting protein)
Length = 615
Score = 33.1 bits (74), Expect = 2.2
Identities = 23/76 (30%), Positives = 36/76 (47%), Gaps = 5/76 (6%)
Query: 313 KVNYKPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQK-----MTDTESCRSRQ 367
K + ++ K+ E E+R E A +L E+K+ + E A W K MT+ + S
Sbjct: 434 KEEWTLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWVKQQFLNMTNFDHQNSEN 493
Query: 368 DDSSVEFCESSDPDDV 383
F SSDPD++
Sbjct: 494 VKLFSAFSGSSDPDNL 509
>YJJK_HAEIN (P45127) ABC transporter ATP-binding protein HI1252
Length = 556
Score = 32.7 bits (73), Expect = 2.8
Identities = 20/58 (34%), Positives = 31/58 (52%), Gaps = 8/58 (13%)
Query: 303 WVLS---GPPINWKVNYKPWQRPKEE-LEEEQ----RKIEEVAKKLCWEKKSEKAEIA 352
W+L G I W+ NY W KE+ LE+EQ + + +AK+L W +++ K A
Sbjct: 229 WILELDRGEGIPWEGNYSSWLEQKEKRLEQEQATENARQKSIAKELEWVRQNPKGRQA 286
>GUNB_NEOPA (Q12647) Endoglucanase B precursor (EC 3.2.1.4)
(Endo-1,4-beta-glucanase B) (Cellulase B)
Length = 473
Score = 32.7 bits (73), Expect = 2.8
Identities = 26/83 (31%), Positives = 36/83 (43%), Gaps = 22/83 (26%)
Query: 183 QFPQGADKYIDQLASVIPINDGTVRTALDTGCGVASW---GAYL-WSRNVVAMSFAPRDS 238
+FP G DK I L S P N AL+ G G S G+ + W+ N + F
Sbjct: 235 KFPSGDDKVIVSLHSYSPYN-----FALNNGAGAISNFYDGSEIDWAMNTINSKF----- 284
Query: 239 HEAQVQFALERGVPAVIGVFGTI 261
+ RG+P +IG FG +
Sbjct: 285 --------ISRGIPVIIGEFGAM 299
>ADIP_RAT (Q8CGZ2) Afadin- and alpha-actinin-binding protein (ADIP)
(Afadin DIL domain-interacting protein)
Length = 613
Score = 32.7 bits (73), Expect = 2.8
Identities = 23/76 (30%), Positives = 35/76 (45%), Gaps = 5/76 (6%)
Query: 313 KVNYKPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQK-----MTDTESCRSRQ 367
K + ++ K+ E E+R E A +L E+K+ + E A W K MT + S
Sbjct: 434 KEEWSLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWVKQQFLNMTTFDHQNSEN 493
Query: 368 DDSSVEFCESSDPDDV 383
F SSDPD++
Sbjct: 494 VKLFSAFSGSSDPDNL 509
>CHD2_HUMAN (O14647) Chromodomain-helicase-DNA-binding protein 2
(CHD-2)
Length = 1739
Score = 32.3 bits (72), Expect = 3.7
Identities = 25/75 (33%), Positives = 36/75 (47%), Gaps = 3/75 (4%)
Query: 308 PPINWKVNYKPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQKMTDTESCRSRQ 367
P +W QR K E EE Q+++EE+ ++KA+ +DTES R Q
Sbjct: 1039 PHKDWDEIIPEEQRKKVEEEERQKELEEIYMLPRIRSSTKKAQTN--DSDSDTESKRQAQ 1096
Query: 368 DDSSVEFCESSDPDD 382
S+ E E+ D DD
Sbjct: 1097 RSSASE-SETEDSDD 1110
>YJJK_ECOLI (P37797) ABC transporter ATP-binding protein yjjK
Length = 554
Score = 32.0 bits (71), Expect = 4.8
Identities = 20/74 (27%), Positives = 35/74 (47%), Gaps = 15/74 (20%)
Query: 303 WVLS---GPPINWKVNYKPW-----QRPKEELEEEQRKIEEVAKKLCWEKKSE------- 347
W+L G I W+ NY W QR +E +E + + + K+L W ++
Sbjct: 227 WILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWVRQGTKGRQSKG 286
Query: 348 KAEIAIWQKMTDTE 361
KA +A ++++ TE
Sbjct: 287 KARLARFEELNSTE 300
>BIOC_ECOLI (P12999) Biotin synthesis protein bioC
Length = 251
Score = 32.0 bits (71), Expect = 4.8
Identities = 35/131 (26%), Positives = 54/131 (40%), Gaps = 15/131 (11%)
Query: 177 FPGGGTQFPQGAD---KYIDQLASVIPINDGTVRTALDTGCGVASWGAYLWSRN---VVA 230
F + Q AD + D L +++P T LD GCG W + W V A
Sbjct: 13 FGRAAAHYEQHADLQRQSADALLAMLPQRKYT--HVLDAGCG-PGWMSRHWRERHAQVTA 69
Query: 231 MSFAPRDSHEAQVQFALERGVPAVIGVFGTIKLPYPSRAFDMAHCSRCLIPWGANDGMYM 290
+ +P +A+ + A + + I LP + FD+A S + W N +
Sbjct: 70 LDLSPPMLVQARQKDAADHYLAGDIE-----SLPLATATFDLAW-SNLAVQWCGNLSTAL 123
Query: 291 MEVDRVLRPGG 301
E+ RV+RP G
Sbjct: 124 RELYRVVRPKG 134
>SYH_YEAST (P07263) Histidyl-tRNA synthetase, mitochondrial
precursor (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS)
Length = 546
Score = 31.6 bits (70), Expect = 6.3
Identities = 35/158 (22%), Positives = 66/158 (41%), Gaps = 21/158 (13%)
Query: 313 KVNYKPWQRPKEELEEEQRKIEEVAK------KLCWEKKSEKAEIAIWQKMTDTESCRSR 366
K++ PW+ K+E+ EE+ + EE A KL K A ++ +T E +
Sbjct: 239 KLDKSPWEAVKKEMTEEKGQSEETADKIGEYVKLNGSLKEIHAVLSADANITSNEKAKQG 298
Query: 367 QDD--SSVEFCESSDPDDVWYKKLKACVTPTPKVSGGDLKPFPDRLYAIPPRVSSGSIPG 424
DD + +++ E+ D D L L + +Y + V+S S P
Sbjct: 299 LDDIATLMKYTEAFDIDSFISFDLSL---------ARGLDYYTGLIYEV---VTSASAPP 346
Query: 425 VSSETYQNDNKMWKKHVNAYKKINSLLDSGRYRNIMDM 462
++ + K + + + + S+ GRY N+++M
Sbjct: 347 ENASELKKKAKS-AEDASEFVGVGSIAAGGRYDNLVNM 383
>CTB6_HUMAN (Q96HY6) Protein C20orf116 precursor
Length = 314
Score = 31.6 bits (70), Expect = 6.3
Identities = 16/48 (33%), Positives = 24/48 (49%)
Query: 320 QRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQKMTDTESCRSRQ 367
QR EE E E+RK E ++ W+K+ E+ + QK + R Q
Sbjct: 134 QREAEEAEREERKRLESQREAEWKKEEERLRLEEEQKEEEERKAREEQ 181
>AL13_SCHPO (O13953) Cell polarity protein alp13 (Altered polarity
protein 13)
Length = 337
Score = 31.6 bits (70), Expect = 6.3
Identities = 17/67 (25%), Positives = 32/67 (47%)
Query: 305 LSGPPINWKVNYKPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQKMTDTESCR 364
+ G + ++YK W+ +E E+ R ++ + L +K+ + A I+ QK T +S
Sbjct: 29 MKGDVTTYLIHYKGWKNSWDEWVEQDRILQWTEENLKTQKELKNAAISTRQKPTSKKSAS 88
Query: 365 SRQDDSS 371
S S
Sbjct: 89 STSKHDS 95
>TONB_HAEDU (O51810) TonB protein
Length = 279
Score = 31.2 bits (69), Expect = 8.3
Identities = 15/34 (44%), Positives = 24/34 (70%)
Query: 317 KPWQRPKEELEEEQRKIEEVAKKLCWEKKSEKAE 350
KP ++PKE+ +E+ +K E+ +KL EK EKA+
Sbjct: 88 KPIEKPKEKPKEKPKKPEKPKEKLKKEKPKEKAK 121
>INVO_CANFA (P18174) Involucrin
Length = 285
Score = 31.2 bits (69), Expect = 8.3
Identities = 14/52 (26%), Positives = 29/52 (54%)
Query: 322 PKEELEEEQRKIEEVAKKLCWEKKSEKAEIAIWQKMTDTESCRSRQDDSSVE 373
P++ LE++Q + E K+L E++ +K E+ ++ E C Q+ ++E
Sbjct: 136 PEQCLEQQQEQQESQEKELHLEQEQQKEELQQQEQQQGKEQCEKHQEAKNLE 187
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.318 0.136 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,592,707
Number of Sequences: 164201
Number of extensions: 3799858
Number of successful extensions: 9751
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 9739
Number of HSP's gapped (non-prelim): 23
length of query: 610
length of database: 59,974,054
effective HSP length: 116
effective length of query: 494
effective length of database: 40,926,738
effective search space: 20217808572
effective search space used: 20217808572
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 69 (31.2 bits)
Medicago: description of AC146568.1