BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146559.4 - phase: 0 /pseudo
(159 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
FX11_RAT (Q7TSL3) F-box only protein 11 35 0.055
FX11_HUMAN (Q86XK2) F-box only protein 11 (Vitiligo-associated p... 35 0.055
YG46_YEAST (P53301) Hypothetical 52.8 kDa protein in BUB1-HIP1 i... 30 2.3
PR17_MOUSE (Q9DC48) Pre-mRNA splicing factor PRP17 (Cell divisio... 30 2.3
PR17_HUMAN (O60508) Pre-mRNA splicing factor PRP17 (hPRP17) (Cel... 30 2.3
N106_SCHPO (O14310) Nucleoporin npp106 (Nuclear pore protein npp... 30 3.0
SCOB_EMENI (Q00659) Sulfur metabolite repression control protein 29 3.9
FXL8_HUMAN (Q96CD0) F-box/LRR-repeat protein 8 (F-box and leucin... 29 3.9
YEW0_YEAST (P39959) Putative 50.3 kDa zinc finger protein in PAK... 29 5.1
T172_HUMAN (O14981) TBP-associated factor 172 (TAF-172) (TAF(II)... 29 5.1
SRS2_YEAST (P12954) ATP-dependent DNA helicase SRS2 (EC 3.6.1.-) 29 5.1
PEA2_YEAST (P40091) PEA2 protein (PPF2 protein) 29 5.1
MT30_YEAST (P39014) MET30 protein 29 5.1
FXL5_MOUSE (Q8C2S5) F-box/LRR-repeat protein 5 (F-box and leucin... 29 5.1
COQ6_MOUSE (Q8R1S0) Ubiquinone biosynthesis monooxgenase COQ6 (E... 29 5.1
COQ6_HUMAN (Q9Y2Z9) Ubiquinone biosynthesis monooxgenase COQ6 (E... 29 5.1
YDJB_SCHPO (P87059) Hypothetical protein C57A10.11c in chromosome I 28 6.7
TTL4_HUMAN (Q14679) Tubulin tyrosine ligase-like protein 4 28 6.7
APU_THEET (P38939) Amylopullulanase precursor (Alpha-amylase/pul... 28 6.7
RYK_MOUSE (Q01887) Tyrosine-protein kinase RYK precursor (EC 2.7... 28 8.7
>FX11_RAT (Q7TSL3) F-box only protein 11
Length = 843
Score = 35.4 bits (80), Expect = 0.055
Identities = 16/42 (38%), Positives = 21/42 (49%)
Query: 13 EVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDR 54
E LP+ + KI S+ D CR + VCK F A +W R
Sbjct: 73 EKLPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDPILWKR 114
>FX11_HUMAN (Q86XK2) F-box only protein 11 (Vitiligo-associated
protein VIT-1)
Length = 843
Score = 35.4 bits (80), Expect = 0.055
Identities = 16/42 (38%), Positives = 21/42 (49%)
Query: 13 EVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDR 54
E LP+ + KI S+ D CR + VCK F A +W R
Sbjct: 73 EKLPDEVVLKIFSYLLEQDLCRAACVCKRFSELANDPILWKR 114
>YG46_YEAST (P53301) Hypothetical 52.8 kDa protein in BUB1-HIP1
intergenic region
Length = 507
Score = 30.0 bits (66), Expect = 2.3
Identities = 26/95 (27%), Positives = 39/95 (40%), Gaps = 4/95 (4%)
Query: 18 GCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLFSTS 77
G I+ + ++ V S S V S S + S + S +S S S SS STS
Sbjct: 300 GSISSSSTSSSTVSSSASSTVSSSVSSTVSSSA--SSTVSSSVSSTVSSSSSVSSSSSTS 357
Query: 78 PSKKSL--YLTLSDHPIVIENGKKSFQLEKQSGRK 110
PS + TL+ + + SF+ + S K
Sbjct: 358 PSSSTATSSKTLASSSVTTSSSISSFEKQSSSSSK 392
>PR17_MOUSE (Q9DC48) Pre-mRNA splicing factor PRP17 (Cell division
cycle 40 homolog)
Length = 579
Score = 30.0 bits (66), Expect = 2.3
Identities = 27/98 (27%), Positives = 43/98 (43%), Gaps = 12/98 (12%)
Query: 47 KSDTVWDRFLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQ 106
KS VW+ +P D I S + + SP+ K L D+ I+I + F+L K+
Sbjct: 440 KSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKK 499
Query: 107 SGRKIYMLS--------ARDISIAL-GD---TPQFWDW 132
K +M++ + D+S + GD WDW
Sbjct: 500 KIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDW 537
>PR17_HUMAN (O60508) Pre-mRNA splicing factor PRP17 (hPRP17) (Cell
division cycle 40 homolog) (EH-binding protein 3) (Ehb3)
Length = 579
Score = 30.0 bits (66), Expect = 2.3
Identities = 27/98 (27%), Positives = 43/98 (43%), Gaps = 12/98 (12%)
Query: 47 KSDTVWDRFLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQ 106
KS VW+ +P D I S + + SP+ K L D+ I+I + F+L K+
Sbjct: 440 KSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKK 499
Query: 107 SGRKIYMLS--------ARDISIAL-GD---TPQFWDW 132
K +M++ + D+S + GD WDW
Sbjct: 500 KIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDW 537
>N106_SCHPO (O14310) Nucleoporin npp106 (Nuclear pore protein
npp106)
Length = 933
Score = 29.6 bits (65), Expect = 3.0
Identities = 16/45 (35%), Positives = 24/45 (52%)
Query: 71 SSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRKIYMLS 115
S ++ + S K+LY T+S P+V E KK Q + G + M S
Sbjct: 866 SLMYISMSSIKALYRTISKLPVVNEESKKKLQRLQFKGSMLVMFS 910
>SCOB_EMENI (Q00659) Sulfur metabolite repression control protein
Length = 678
Score = 29.3 bits (64), Expect = 3.9
Identities = 15/43 (34%), Positives = 20/43 (45%)
Query: 12 IEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDR 54
I LP KIL + C+ S V +G+ + A D VW R
Sbjct: 181 ITALPPEIAFKILCYLDTTSLCKASQVSRGWRALADDDVVWHR 223
>FXL8_HUMAN (Q96CD0) F-box/LRR-repeat protein 8 (F-box and
leucine-rich repeat protein 8) (F-box protein FBL8)
Length = 374
Score = 29.3 bits (64), Expect = 3.9
Identities = 14/40 (35%), Positives = 20/40 (50%)
Query: 13 EVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVW 52
E LPE +A I H + D + VC+ + +AA VW
Sbjct: 6 EGLPEEVLALIFRHLSLRDRAAAARVCRAWAAAATCSAVW 45
>YEW0_YEAST (P39959) Putative 50.3 kDa zinc finger protein in
PAK1-RPS26B intergenic region
Length = 443
Score = 28.9 bits (63), Expect = 5.1
Identities = 19/52 (36%), Positives = 31/52 (59%), Gaps = 5/52 (9%)
Query: 59 DLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQSGRK 110
DL++ IS+ S+ S + SPS +S + S H +V+ KK+ ++K GRK
Sbjct: 332 DLLAAISEPSSSPSPSAPSPSVQS---SSSSHGLVVR--KKTGSMQKTRGRK 378
>T172_HUMAN (O14981) TBP-associated factor 172 (TAF-172)
(TAF(II)170)
Length = 1849
Score = 28.9 bits (63), Expect = 5.1
Identities = 26/111 (23%), Positives = 47/111 (41%), Gaps = 7/111 (6%)
Query: 1 MDMKTEEAKSMIEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDL 60
M+ +E ++++ CIAK+L C S + K CS+ D P
Sbjct: 882 METIKKEENTLVQNYAAQCIAKLLQQCTTRTPCPNSKIIKNLCSSLCVDPY---LTPCVT 938
Query: 61 ISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVI---ENGKKSFQLEKQSG 108
+ + S +S STS K ++ T++ H +I + K +F + + G
Sbjct: 939 CPVPTQSGQENSKGSTS-EKDGMHHTVTKHRGIITLYRHQKAAFAITSRRG 988
>SRS2_YEAST (P12954) ATP-dependent DNA helicase SRS2 (EC 3.6.1.-)
Length = 1175
Score = 28.9 bits (63), Expect = 5.1
Identities = 17/38 (44%), Positives = 22/38 (57%), Gaps = 3/38 (7%)
Query: 54 RFLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHP 91
RFL +DLI +SDS LF ++ S K LY L+ P
Sbjct: 749 RFLTTDLIKAMSDS---QKLFESTNSIKKLYRILNKKP 783
>PEA2_YEAST (P40091) PEA2 protein (PPF2 protein)
Length = 420
Score = 28.9 bits (63), Expect = 5.1
Identities = 22/67 (32%), Positives = 31/67 (45%), Gaps = 1/67 (1%)
Query: 41 GFCSAAKSDTVWDRFLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENGKK- 99
G S A S WD PS + S S S S S TS KK L T + + + ++
Sbjct: 330 GMSSPASSPVTWDPSSPSSVGSPTSGSGSRSLSIMTSEFKKVLTSTQRKYEKELSDEREH 389
Query: 100 SFQLEKQ 106
F+LE++
Sbjct: 390 RFKLERE 396
>MT30_YEAST (P39014) MET30 protein
Length = 640
Score = 28.9 bits (63), Expect = 5.1
Identities = 14/41 (34%), Positives = 20/41 (48%)
Query: 12 IEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVW 52
I +LP+ KILS+ C + VC+ + A D VW
Sbjct: 184 ISILPQELSLKILSYLDCQSLCNATRVCRKWQKLADDDRVW 224
>FXL5_MOUSE (Q8C2S5) F-box/LRR-repeat protein 5 (F-box and
leucine-rich repeat protein 5)
Length = 690
Score = 28.9 bits (63), Expect = 5.1
Identities = 15/56 (26%), Positives = 25/56 (43%)
Query: 2 DMKTEEAKSMIEVLPEGCIAKILSHTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLP 57
+ + E + I LP + I S+ P + CR S V + AK+ ++W P
Sbjct: 195 EAEVSEHSTGITHLPPEVMLSIFSYLNPQELCRCSQVSTKWSQLAKTGSLWKHLYP 250
>COQ6_MOUSE (Q8R1S0) Ubiquinone biosynthesis monooxgenase COQ6 (EC
1.14.13.-)
Length = 469
Score = 28.9 bits (63), Expect = 5.1
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Query: 45 AAKSDTVWDRFLPSDLISIISDSPSASSL-FSTSPSKKSLYLTLSDHPIV 93
A +++ W RFLPS I+++ S + SSL +STS + +++ + V
Sbjct: 242 ATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEEFV 291
>COQ6_HUMAN (Q9Y2Z9) Ubiquinone biosynthesis monooxgenase COQ6 (EC
1.14.13.-) (CGI-10)
Length = 468
Score = 28.9 bits (63), Expect = 5.1
Identities = 16/50 (32%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Query: 45 AAKSDTVWDRFLPSDLISIISDSPSASSL-FSTSPSKKSLYLTLSDHPIV 93
A +++ W RFLPS I+++ S + SSL +STS + +++ + V
Sbjct: 241 ATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFV 290
>YDJB_SCHPO (P87059) Hypothetical protein C57A10.11c in chromosome I
Length = 313
Score = 28.5 bits (62), Expect = 6.7
Identities = 24/76 (31%), Positives = 37/76 (48%), Gaps = 6/76 (7%)
Query: 38 VCKGFCSAAKSDTVWDRFLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENG 97
+C+ F +A K+D RFLP + + + S +A L + S S +DHP+V E+
Sbjct: 36 ICRKFGTATKTDVWKGRFLPI-VSGVAAASLTAGYLLGKNTSNASSSQD-NDHPVVGEH- 92
Query: 98 KKSFQLEKQSGRKIYM 113
E QS + YM
Sbjct: 93 ---VHTETQSYNEPYM 105
>TTL4_HUMAN (Q14679) Tubulin tyrosine ligase-like protein 4
Length = 1199
Score = 28.5 bits (62), Expect = 6.7
Identities = 19/74 (25%), Positives = 32/74 (42%)
Query: 48 SDTVWDRFLPSDLISIISDSPSASSLFSTSPSKKSLYLTLSDHPIVIENGKKSFQLEKQS 107
SD +W++ + +IIS P +SL + L I+++ K + LE
Sbjct: 850 SDAIWEKIKDVVVKTIISSEPYVTSLLKMYVRRPYSCHELFGFDIMLDENLKPWVLEVNI 909
Query: 108 GRKIYMLSARDISI 121
++ S DISI
Sbjct: 910 SPSLHSSSPLDISI 923
>APU_THEET (P38939) Amylopullulanase precursor
(Alpha-amylase/pullulanase) [Includes: Alpha-amylase (EC
3.2.1.1) (1,4-alpha-D-glucan glucanohydrolase);
Pullulanase (EC 3.2.1.41) (1,4-alpha-D-glucan
glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosida
Length = 1481
Score = 28.5 bits (62), Expect = 6.7
Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 15/92 (16%)
Query: 67 SPSASSLFSTSPSKKSLYLTLSDHPIV-----------IENGKKSFQLEKQSGRKIYMLS 115
+PSA + T P+ S+ LTL++ + +E G+ E+ + RKI +++
Sbjct: 1284 NPSAHQMTKTGPNTYSITLTLNEGTQLEYKYARGSWDKVEKGEYG---EEIANRKITVVN 1340
Query: 116 ARDISIALGDTPQFW-DWPILPESRCVNCVID 146
++ + DT Q W D PI S N +D
Sbjct: 1341 QGSNTMVVNDTVQRWRDLPIYIYSPKDNTTVD 1372
>RYK_MOUSE (Q01887) Tyrosine-protein kinase RYK precursor (EC
2.7.1.112) (Kinase VIK) (NYK-R) (Met-related kinase)
Length = 594
Score = 28.1 bits (61), Expect = 8.7
Identities = 29/118 (24%), Positives = 51/118 (42%), Gaps = 28/118 (23%)
Query: 26 HTAPVDSCRLSLVCKGFCSAAKSDTVWDRFLPSDLISII------------SDSPSASSL 73
H AP S R+ + G C A FL + +++++ S S S+SS
Sbjct: 203 HAAPTTSTRVFYISVGVCCAV-------IFLVAIILAVLHLHSMKRIELDDSISASSSSQ 255
Query: 74 FSTSPSKKSLYLTLSD-----HPIVIENGKKSFQLEKQSGRKIYMLSA----RDISIA 122
+ PS ++ +D PI +G + ++EK R + +L A +DI+I+
Sbjct: 256 GLSQPSTQTTQYLRADTPNNATPITSSSGYPTLRIEKNDLRSVTLLEAKAKVKDIAIS 313
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,981,889
Number of Sequences: 164201
Number of extensions: 682819
Number of successful extensions: 1697
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1685
Number of HSP's gapped (non-prelim): 22
length of query: 159
length of database: 59,974,054
effective HSP length: 101
effective length of query: 58
effective length of database: 43,389,753
effective search space: 2516605674
effective search space used: 2516605674
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC146559.4