BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146343.4 - phase: 0
(141 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
COLA_VIBPA (Q56696) Microbial collagenase precursor (EC 3.4.24.3... 32 0.58
PLYA_COLGL (Q00374) Pectin lyase precursor (EC 4.2.2.10) 31 0.75
PGL4_ASPNG (O42809) Polygalacturonase IV precursor (EC 3.2.1.15)... 30 1.7
YA02_METJA (Q58408) Hypothetical protein MJ1002 29 2.9
FRDA_HUMAN (Q16595) Frataxin, mitochondrial precursor (Friedreic... 29 2.9
COBT_CHRVO (Q7P0S3) Nicotinate-nucleotide--dimethylbenzimidazole... 29 3.7
PGLB_ASPFL (P41750) Polygalacturonase B precursor (EC 3.2.1.15) ... 28 4.9
PGK2_METJA (Q58877) 2-phosphoglycerate kinase (EC 2.7.2.-) (2PGK) 28 4.9
NTP1_CFEPV (O91729) Nucleoside triphosphatase I (EC 3.6.1.15) (N... 28 4.9
ARTI_ECOLI (P30859) Arginine-binding periplasmic protein 1 precu... 28 4.9
NTP1_CBEPV (P24486) Nucleoside triphosphatase I (EC 3.6.1.15) (N... 28 6.4
HLYB_ACTAC (P23702) Leukotoxin translocation ATP-binding protein... 28 6.4
YLA1_LACAC (P29470) Hypothetical protein in laf 5'region (ORF1) ... 28 8.3
UL52_HHV6U (P52467) DNA helicase/primase complex protein 28 8.3
S6AH_HUMAN (Q9H1V8) Orphan sodium- and chloride-dependent neurot... 28 8.3
RIR2_BPT4 (P11156) Ribonucleoside-diphosphate reductase beta cha... 28 8.3
>COLA_VIBPA (Q56696) Microbial collagenase precursor (EC 3.4.24.3)
(prtVp)
Length = 816
Score = 31.6 bits (70), Expect = 0.58
Identities = 17/37 (45%), Positives = 21/37 (55%)
Query: 103 ASWTEYPELGVLGDLLASPDTNTKASILVVQQQVMPK 139
A++ + L G LLASPD TK L V QQVM +
Sbjct: 240 ANFIVFNALRETGRLLASPDQETKRKALAVMQQVMQR 276
>PLYA_COLGL (Q00374) Pectin lyase precursor (EC 4.2.2.10)
Length = 380
Score = 31.2 bits (69), Expect = 0.75
Identities = 28/113 (24%), Positives = 50/113 (43%), Gaps = 14/113 (12%)
Query: 28 TIKNDEF---TSWDVMLDDELLKGVKGVYGSVDNVTYEKVEVENTSDTTEFISDIAIQH- 83
TI N+E TSW D+ GV + GS D VT+ K + +TS + I+ ++ H
Sbjct: 211 TISNNEIDGSTSWSATCDNHHYWGVY-LTGSNDMVTFSKNYIHHTSGRSPKIAGNSLVHI 269
Query: 84 --DFIIVGRRNGIKSP-------QTQALASWTEYPELGVLGDLLASPDTNTKA 127
++ + +++ + + + G+ G + +SPD NT A
Sbjct: 270 SGNYFYANSGHAMEADAGAKVVLEGNVFQNVVAAMQSGLAGKVFSSPDANTNA 322
>PGL4_ASPNG (O42809) Polygalacturonase IV precursor (EC 3.2.1.15)
(PG-IV) (Pectinase 4)
Length = 378
Score = 30.0 bits (66), Expect = 1.7
Identities = 22/86 (25%), Positives = 39/86 (44%), Gaps = 13/86 (15%)
Query: 27 STIKNDEFTSWDVMLDDE--LLKGVKGVYGSVDNVTYEKVEV-----------ENTSDTT 73
+T+KN F D+ +K + G GSV VTY+++ + +N DT+
Sbjct: 254 NTVKNVTFYDSDIKSSQNGVRIKTIYGDTGSVSEVTYKEITLSDITDYGIVVEQNYDDTS 313
Query: 74 EFISDIAIQHDFIIVGRRNGIKSPQT 99
E +D DF++ + ++S T
Sbjct: 314 ESPTDGITIEDFVLDNVQGSVESSGT 339
>YA02_METJA (Q58408) Hypothetical protein MJ1002
Length = 309
Score = 29.3 bits (64), Expect = 2.9
Identities = 21/59 (35%), Positives = 30/59 (50%), Gaps = 4/59 (6%)
Query: 25 LVSTIKNDEFTSWDVMLDDELLKGVKGVYGSVDNVTYEKVEVENTSDTTEFISDIAIQH 83
L S +K DE + +LD K VK + + + VTY+K+ EN E I I I+H
Sbjct: 35 LKSFLKKDEHSGIIKILDKISHKNVKIIVATPNQVTYKKISSEN----EENIYPIFIKH 89
>FRDA_HUMAN (Q16595) Frataxin, mitochondrial precursor (Friedreich's
ataxia protein) (Fxn)
Length = 210
Score = 29.3 bits (64), Expect = 2.9
Identities = 13/33 (39%), Positives = 17/33 (51%)
Query: 48 GVKGVYGSVDNVTYEKVEVENTSDTTEFISDIA 80
G G GS+D TYE++ E EF D+A
Sbjct: 82 GTLGHPGSLDETTYERLAEETLDSLAEFFEDLA 114
>COBT_CHRVO (Q7P0S3) Nicotinate-nucleotide--dimethylbenzimidazole
phosphoribosyltransferase (EC 2.4.2.21) (NN:DBI PRT)
(N(1)-alpha-phosphoribosyltransferase)
Length = 344
Score = 28.9 bits (63), Expect = 3.7
Identities = 26/93 (27%), Positives = 39/93 (40%), Gaps = 9/93 (9%)
Query: 34 FTSWDVMLDDELLKGVKGVYGSVDNVTYEKVEVENTSDTTEFISDIAIQHDFIIVGRRNG 93
F +W E L+ V+ + VT E V ++ TTE + + A I V R
Sbjct: 39 FAAWQGRAQPEDLRPAITVFAADHGVTVEGVSAFPSAVTTEMVRNFANGGAAICVLAR-- 96
Query: 94 IKSPQTQALASWTEYPELGVLGDLLASPDTNTK 126
AL + E ++GV GD+ A P + K
Sbjct: 97 -------ALDARLEVVDVGVAGDVSALPIVHAK 122
>PGLB_ASPFL (P41750) Polygalacturonase B precursor (EC 3.2.1.15)
(Pectinase) (PGL) (P1/P3)
Length = 366
Score = 28.5 bits (62), Expect = 4.9
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 2/44 (4%)
Query: 27 STIKNDEFTSWDVMLDDE--LLKGVKGVYGSVDNVTYEKVEVEN 68
+T+KN + V+ D +K + G GSV VTYE + ++N
Sbjct: 240 NTVKNVKVADSTVVDSDNGIRIKTISGATGSVSGVTYENITLKN 283
>PGK2_METJA (Q58877) 2-phosphoglycerate kinase (EC 2.7.2.-) (2PGK)
Length = 309
Score = 28.5 bits (62), Expect = 4.9
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 13/76 (17%)
Query: 25 LVSTIKNDEFTSWDVMLDDELLKGVK-----------GVYGSVDN--VTYEKVEVENTSD 71
L+ T+ +T+W V+ DDE K +K GV G +D V + V +E T
Sbjct: 139 LIPTLYESSYTAWKVLRDDEGNKYIKGFERHSEAVLTGVEGVIDRCLVEGQSVIIEGTHL 198
Query: 72 TTEFISDIAIQHDFII 87
+ D +++ ++
Sbjct: 199 VPTLLKDKYLENSHVV 214
>NTP1_CFEPV (O91729) Nucleoside triphosphatase I (EC 3.6.1.15)
(Nucleoside triphosphate phosphohydrolase I) (NPH I)
Length = 647
Score = 28.5 bits (62), Expect = 4.9
Identities = 13/45 (28%), Positives = 24/45 (52%)
Query: 52 VYGSVDNVTYEKVEVENTSDTTEFISDIAIQHDFIIVGRRNGIKS 96
+ G +DNV Y ++ ++ +EF +I +FI + N IK+
Sbjct: 312 IKGDLDNVEYNEMIKRKLAEFSEFTKNINFSKEFINAFKNNEIKT 356
>ARTI_ECOLI (P30859) Arginine-binding periplasmic protein 1
precursor
Length = 243
Score = 28.5 bits (62), Expect = 4.9
Identities = 25/91 (27%), Positives = 41/91 (44%), Gaps = 14/91 (15%)
Query: 45 LLKGVKGVYGSVDNVTYEKVEVENTSDTTEFISD-----IAIQHDF-------IIVGRRN 92
L G +G Y SVD + +KV V+N + +FI D + +D + GR +
Sbjct: 110 LFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAKLDLQNGRID 169
Query: 93 GIKSPQTQALASW-TEYPELGVLGDLLASPD 122
G+ T + W + P+L +GD + D
Sbjct: 170 GVFG-DTAVVTEWLKDNPKLAAVGDKVTDKD 199
>NTP1_CBEPV (P24486) Nucleoside triphosphatase I (EC 3.6.1.15)
(Nucleoside triphosphate phosphohydrolase I) (NPH I)
Length = 648
Score = 28.1 bits (61), Expect = 6.4
Identities = 14/45 (31%), Positives = 23/45 (51%)
Query: 52 VYGSVDNVTYEKVEVENTSDTTEFISDIAIQHDFIIVGRRNGIKS 96
+ G +DNV Y ++ ++ +EF +I FI + N IKS
Sbjct: 313 IKGDLDNVEYNEMIKRKLAEFSEFTKNINFSKAFINAFKNNEIKS 357
>HLYB_ACTAC (P23702) Leukotoxin translocation ATP-binding protein
lktB
Length = 707
Score = 28.1 bits (61), Expect = 6.4
Identities = 15/46 (32%), Positives = 25/46 (53%), Gaps = 4/46 (8%)
Query: 94 IKSPQTQALASWTEYPELGV----LGDLLASPDTNTKASILVVQQQ 135
I SP + W ++ ++G+ LGD+L SP N AS+ + + Q
Sbjct: 420 IISPVIRLAQIWQDFQQVGISVTRLGDVLNSPTENNTASVSLPEIQ 465
>YLA1_LACAC (P29470) Hypothetical protein in laf 5'region (ORF1)
(Fragment)
Length = 188
Score = 27.7 bits (60), Expect = 8.3
Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 4/86 (4%)
Query: 9 LKGQSKKILITWVVYHLVSTIKNDEFTSWDVMLDDELLKGVKGVYGSVDNVTYEKVEVEN 68
+K +SK ++T+V +STIK + ++ V D L + + G V+ + V + N
Sbjct: 86 MKPKSKVKIVTYVTSDKISTIKKGQSLTFSVPTGDGL---TRHLTGKVEKIGVYPVNM-N 141
Query: 69 TSDTTEFISDIAIQHDFIIVGRRNGI 94
+ E IS + + I G + +
Sbjct: 142 KQNIYEIISTAKVNDENIKYGMQGNV 167
>UL52_HHV6U (P52467) DNA helicase/primase complex protein
Length = 860
Score = 27.7 bits (60), Expect = 8.3
Identities = 21/85 (24%), Positives = 38/85 (44%), Gaps = 8/85 (9%)
Query: 27 STIKNDEFTSWDVMLDDELLKGVKGVYGSVDNVTYE----KVEVENTSDTTEFISDIAIQ 82
+TI + F S ++ L+ K ++ S+D T+ K + + T+DT ++ D
Sbjct: 501 ATITEETFFSMCRLIRLTLINAWKKIFPSIDTDTHPIFFFKTQCDTTNDTLDYTEDPTEI 560
Query: 83 HDFII----VGRRNGIKSPQTQALA 103
F + +G R I P A+A
Sbjct: 561 KQFCVCRKKIGLRISIPLPNGTAIA 585
>S6AH_HUMAN (Q9H1V8) Orphan sodium- and chloride-dependent
neurotransmitter transporter NTT4 (Solute carrier family
6 member 17) (Fragment)
Length = 439
Score = 27.7 bits (60), Expect = 8.3
Identities = 12/33 (36%), Positives = 24/33 (72%), Gaps = 3/33 (9%)
Query: 22 VYHLVSTIKNDEFTSWDV---MLDDELLKGVKG 51
+Y+++ T+K D+F++ + +L+DEL K V+G
Sbjct: 117 MYNVIMTVKEDQFSALGLDPCLLEDELDKSVQG 149
>RIR2_BPT4 (P11156) Ribonucleoside-diphosphate reductase beta chain
(EC 1.17.4.1) (Ribonucleotide reductase) (B2 protein)
Length = 388
Score = 27.7 bits (60), Expect = 8.3
Identities = 14/56 (25%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Query: 24 HLVSTIKNDEFTSWD-VMLDDELLKGVKGVYGSVDNVTYEKVEVENTSDTTEFISD 78
H++ + D +D ++LD+ ++K + + D+V + E EN D E+ D
Sbjct: 124 HIMRNLYTDPSKVFDEIVLDEAIMKRAESIGRYYDDVLVKTREWENAKDMVEYYKD 179
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.133 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,846,379
Number of Sequences: 164201
Number of extensions: 643423
Number of successful extensions: 1759
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1751
Number of HSP's gapped (non-prelim): 16
length of query: 141
length of database: 59,974,054
effective HSP length: 99
effective length of query: 42
effective length of database: 43,718,155
effective search space: 1836162510
effective search space used: 1836162510
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC146343.4