BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146332.8 - phase: 0 /pseudo
(199 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CRK7_HUMAN (Q9NYV4) Cell division cycle 2-related protein kinase... 33 0.56
CYAA_NEUCR (Q01631) Adenylate cyclase (EC 4.6.1.1) (ATP pyrophos... 32 0.74
YD38_YEAST (Q05497) Hypothetical 77.8 kDa protein in MRPS28-HXT7... 30 2.8
AXN1_RAT (O70239) Axin 1 protein (Axis inhibition protein 1) (rA... 30 2.8
MY3A_MOUSE (Q8K3H5) Myosin IIIA (EC 2.7.1.37) 30 3.7
GOA3_HUMAN (Q08378) Golgi autoantigen, golgin subfamily A member... 30 3.7
GAT2_CHICK (P23824) GATA binding factor-2 (Transcription factor ... 30 3.7
XTH_SOYBN (Q39857) Probable xyloglucan endotransglucosylase/hydr... 30 4.8
XTHA_PHAAN (Q41638) Xyloglucan endotransglucosylase/hydrolase pr... 30 4.8
RP24_YEAST (Q07915) Ribosome biogenesis protein RLP24 (Ribosomal... 30 4.8
DMD_CAEEL (Q9TW65) Dystrophin-1 30 4.8
RP24_KLULA (Q6CNW3) Ribosome biogenesis protein RLP24 29 8.2
RP24_CANGA (Q6FPU0) Ribosome biogenesis protein RLP24 29 8.2
PPQ1_YEAST (P32945) Serine/threonine protein phosphatase PPQ (EC... 29 8.2
EPPL_HUMAN (P58107) Epiplakin (450 kDa epidermal antigen) 29 8.2
BEM2_YEAST (P39960) GTPase activating protein BEM2/IPL2 29 8.2
>CRK7_HUMAN (Q9NYV4) Cell division cycle 2-related protein kinase 7
(EC 2.7.1.37) (CDC2-related protein kinase 7) (CrkRS)
Length = 1490
Score = 32.7 bits (73), Expect = 0.56
Identities = 32/123 (26%), Positives = 55/123 (44%), Gaps = 17/123 (13%)
Query: 22 SSTSSSSPFSRRYRFKRAILMGKKSGY--------NTPAPMWKTSSKSPSMATHHHHAMK 73
SST S SP+SRR R + S Y +P+P + S SP ++ ++
Sbjct: 287 SSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLS---KRSLS 343
Query: 74 KTALHS-----SRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIK-KESMKSNKERDK 127
++ L S SR+ P + S S +K +++ + ++P R+ S+ + R K
Sbjct: 344 RSPLPSRKSMKSRSRSPAYSRHSSSHSKKKRSSSRSRHSSISPVRLPLNSSLGAELSRKK 403
Query: 128 VER 130
ER
Sbjct: 404 KER 406
>CYAA_NEUCR (Q01631) Adenylate cyclase (EC 4.6.1.1) (ATP
pyrophosphate-lyase) (Adenylyl cyclase)
Length = 2300
Score = 32.3 bits (72), Expect = 0.74
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 6/55 (10%)
Query: 12 KHNKIR------KRGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSK 60
KHNK K S S++ F+R +R + +MG +S +PAP W +S+
Sbjct: 481 KHNKSNEDPRALKPSLSREDSAASFARDFRNGSSSMMGTRSRAQSPAPSWTGTSR 535
>YD38_YEAST (Q05497) Hypothetical 77.8 kDa protein in MRPS28-HXT7
intergenic region
Length = 695
Score = 30.4 bits (67), Expect = 2.8
Identities = 15/47 (31%), Positives = 21/47 (43%)
Query: 137 LGPQKLDPLVSPFSERTKGIEVDGCKRNVSDLSHQFHFVDPYFRGMD 183
L P K +PLV F RT+ I+ + D H +PY +D
Sbjct: 35 LPPNKKNPLVRKFRARTRNIDQRSFRSLTDDFGSNVHEPNPYLGNID 81
>AXN1_RAT (O70239) Axin 1 protein (Axis inhibition protein 1)
(rAxin)
Length = 827
Score = 30.4 bits (67), Expect = 2.8
Identities = 28/91 (30%), Positives = 37/91 (39%), Gaps = 8/91 (8%)
Query: 16 IRKRGCSSTSSS--SPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPSMATHHHHAMK 73
+R GC S SP S K A+L G SG+ AP K K S HHH +
Sbjct: 478 MRTPGCQSPGPGHRSPDSGHVA-KTAVLGGTASGHGKHAP--KLGLKLDSAGLHHHRHVH 534
Query: 74 KTALHSSRTGLPFKEKKEISVSARKLAATLW 104
H+S KE+ E + R ++ W
Sbjct: 535 HHVHHNSARP---KEQMEAEAARRVQSSFSW 562
>MY3A_MOUSE (Q8K3H5) Myosin IIIA (EC 2.7.1.37)
Length = 1613
Score = 30.0 bits (66), Expect = 3.7
Identities = 22/79 (27%), Positives = 36/79 (44%), Gaps = 7/79 (8%)
Query: 51 PAPMWKTSSKSPSMATHHHHAMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLT 110
P P + K+ S+ + + + KK L R PFK +K +S S T +++
Sbjct: 1336 PVPQAQEEHKAVSIHSKYQSSKKKQQLGKDRLAPPFKNQKILSSSTEVAKTT----HNVY 1391
Query: 111 PSRIKKESMKSNK---ERD 126
P K+E + +K ERD
Sbjct: 1392 PCPTKQEGVHHSKMVDERD 1410
>GOA3_HUMAN (Q08378) Golgi autoantigen, golgin subfamily A member 3
(Golgin-160) (Golgi complex-associated protein of 170
kDa) (GCP170)
Length = 1498
Score = 30.0 bits (66), Expect = 3.7
Identities = 19/74 (25%), Positives = 39/74 (52%), Gaps = 8/74 (10%)
Query: 72 MKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERDKV--- 128
M++T L S+ + LP ++++++ + ARK W L R+K++ +S++ K
Sbjct: 130 MQETQLCSTDSPLPLEKEEQVRLQARK-----WLEEQLKQYRVKRQQERSSQPATKTRLF 184
Query: 129 ERLCRSVLLGPQKL 142
L ++L P+ L
Sbjct: 185 STLDPELMLNPENL 198
>GAT2_CHICK (P23824) GATA binding factor-2 (Transcription factor
NF-E1B) (GATA-2)
Length = 466
Score = 30.0 bits (66), Expect = 3.7
Identities = 21/68 (30%), Positives = 31/68 (44%), Gaps = 8/68 (11%)
Query: 49 NTPAPMWKTSSKSPSMATHHH----HAMKKTALHSSRTGLPFKEKKEISVSARKLAATLW 104
++P W SSK+ A HH+ + KT LH S G P ISV ++
Sbjct: 91 HSPGIPWLDSSKAALSAHHHNPWTVNPFTKTPLHPSAAGAP----GAISVYPGSSTSSTA 146
Query: 105 EINDLTPS 112
++ LTP+
Sbjct: 147 SVSSLTPA 154
>XTH_SOYBN (Q39857) Probable xyloglucan
endotransglucosylase/hydrolase precursor (EC 2.4.1.207)
(Fragment)
Length = 295
Score = 29.6 bits (65), Expect = 4.8
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 4/51 (7%)
Query: 138 GPQKLDPLVSPFSERTKGIEVDGCKRNVS----DLSHQFHFVDPYFRGMDA 184
G +K D +PF KG +DGC+ +V+ D + + P FR +DA
Sbjct: 206 GLEKTDWSKAPFIAAYKGFHIDGCEASVNAKFCDTQGKRWWDQPEFRDLDA 256
>XTHA_PHAAN (Q41638) Xyloglucan endotransglucosylase/hydrolase
protein A precursor (EC 2.4.1.207) (VaXTH1)
Length = 292
Score = 29.6 bits (65), Expect = 4.8
Identities = 17/51 (33%), Positives = 26/51 (50%), Gaps = 4/51 (7%)
Query: 138 GPQKLDPLVSPFSERTKGIEVDGCKRNVS----DLSHQFHFVDPYFRGMDA 184
G +K D +PF KG +DGC+ +V+ D + + P FR +DA
Sbjct: 204 GLEKTDWSKAPFIASYKGFHIDGCEASVNAKFCDTQGKRWWDQPEFRDLDA 254
>RP24_YEAST (Q07915) Ribosome biogenesis protein RLP24 (Ribosomal
protein L24-like)
Length = 199
Score = 29.6 bits (65), Expect = 4.8
Identities = 15/56 (26%), Positives = 25/56 (43%)
Query: 71 AMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERD 126
A+ T + R +P + +E+ + K A + EI K M+ NKE+D
Sbjct: 64 AVDSTLTFAQRRNVPVRYNRELVATTLKAMARIEEIRQKRERAFYKNRMRGNKEKD 119
>DMD_CAEEL (Q9TW65) Dystrophin-1
Length = 3674
Score = 29.6 bits (65), Expect = 4.8
Identities = 27/102 (26%), Positives = 45/102 (43%), Gaps = 3/102 (2%)
Query: 89 KKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSP 148
KK + ++ ++LAA E P +++ + SN E + E+ V +K PL P
Sbjct: 873 KKGLEIAGKRLAALKVEEK---PVEKEEQLVLSNSEEPEAEKHVTFVQETTEKPAPLQEP 929
Query: 149 FSERTKGIEVDGCKRNVSDLSHQFHFVDPYFRGMDAGCNADL 190
SE E+DG V D+ H + R +D N+ +
Sbjct: 930 TSEAQLLEELDGPWSRVGDVVAIEHDLLRAKRAVDTARNSQM 971
>RP24_KLULA (Q6CNW3) Ribosome biogenesis protein RLP24
Length = 198
Score = 28.9 bits (63), Expect = 8.2
Identities = 15/56 (26%), Positives = 24/56 (42%)
Query: 71 AMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERD 126
A+ T + R +P + +E+ + K + + EI K MK N ERD
Sbjct: 64 AVDSTLTFAQRRNVPVRYNRELVATTLKAMSRIEEIRQRRERAFYKNRMKGNNERD 119
>RP24_CANGA (Q6FPU0) Ribosome biogenesis protein RLP24
Length = 197
Score = 28.9 bits (63), Expect = 8.2
Identities = 15/56 (26%), Positives = 24/56 (42%)
Query: 71 AMKKTALHSSRTGLPFKEKKEISVSARKLAATLWEINDLTPSRIKKESMKSNKERD 126
A+ T + R +P + +E+ + K + + EI K MK N ERD
Sbjct: 64 AVDSTLTFAQRRNVPVRYNRELVATTLKAMSRIEEIRQKRERAFYKNRMKGNSERD 119
>PPQ1_YEAST (P32945) Serine/threonine protein phosphatase PPQ (EC
3.1.3.16)
Length = 549
Score = 28.9 bits (63), Expect = 8.2
Identities = 16/39 (41%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Query: 143 DPLVSPFSERTKGIEVDGCKRNVSDLSHQFHFVDPYFRG 181
DP V+ +SE +G+ KRNV D +F F D RG
Sbjct: 447 DPQVTDWSENDRGVSYTFSKRNVLDFCAKFKF-DLILRG 484
>EPPL_HUMAN (P58107) Epiplakin (450 kDa epidermal antigen)
Length = 5065
Score = 28.9 bits (63), Expect = 8.2
Identities = 19/71 (26%), Positives = 30/71 (41%)
Query: 99 LAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGIEV 158
L A L + + T R+ K S + + + L S +LGP+ L L + E+
Sbjct: 4265 LVAVLTRVIEETEERLSKVSFRGLRRQVSASELHTSGILGPETLRDLAQGTKTLQEVTEM 4324
Query: 159 DGCKRNVSDLS 169
D KR + S
Sbjct: 4325 DSVKRYLEGTS 4335
Score = 28.9 bits (63), Expect = 8.2
Identities = 19/71 (26%), Positives = 30/71 (41%)
Query: 99 LAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGIEV 158
L A L + + T R+ K S + + + L S +LGP+ L L + E+
Sbjct: 4799 LVAVLTRVIEETEERLSKVSFRGLRRQVSASELHTSGILGPETLRDLAQGTKTLQEVTEM 4858
Query: 159 DGCKRNVSDLS 169
D KR + S
Sbjct: 4859 DSVKRYLEGTS 4869
Score = 28.9 bits (63), Expect = 8.2
Identities = 19/71 (26%), Positives = 30/71 (41%)
Query: 99 LAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGIEV 158
L A L + + T R+ K S + + + L S +LGP+ L L + E+
Sbjct: 2663 LVAVLTRVIEETEERLSKVSFRGLRRQVSASELHTSGILGPETLRDLAQGTKTLQEVTEM 2722
Query: 159 DGCKRNVSDLS 169
D KR + S
Sbjct: 2723 DSVKRYLEGTS 2733
Score = 28.9 bits (63), Expect = 8.2
Identities = 19/71 (26%), Positives = 30/71 (41%)
Query: 99 LAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGIEV 158
L A L + + T R+ K S + + + L S +LGP+ L L + E+
Sbjct: 3197 LVAVLTRVIEETEERLSKVSFRGLRRQVSASELHTSGILGPETLRDLAQGTKTLQEVTEM 3256
Query: 159 DGCKRNVSDLS 169
D KR + S
Sbjct: 3257 DSVKRYLEGTS 3267
Score = 28.9 bits (63), Expect = 8.2
Identities = 19/71 (26%), Positives = 30/71 (41%)
Query: 99 LAATLWEINDLTPSRIKKESMKSNKERDKVERLCRSVLLGPQKLDPLVSPFSERTKGIEV 158
L A L + + T R+ K S + + + L S +LGP+ L L + E+
Sbjct: 3731 LVAVLTRVIEETEERLSKVSFRGLRRQVSASELHTSGILGPETLRDLAQGTKTLQEVTEM 3790
Query: 159 DGCKRNVSDLS 169
D KR + S
Sbjct: 3791 DSVKRYLEGTS 3801
>BEM2_YEAST (P39960) GTPase activating protein BEM2/IPL2
Length = 2167
Score = 28.9 bits (63), Expect = 8.2
Identities = 17/57 (29%), Positives = 24/57 (41%)
Query: 14 NKIRKRGCSSTSSSSPFSRRYRFKRAILMGKKSGYNTPAPMWKTSSKSPSMATHHHH 70
+K RK +S SSSS + + ++ TS SP M +HHHH
Sbjct: 7 SKNRKSSTASASSSSTSTSHKTTTASTASSSSPSSSSQTIRNSTSGASPYMHSHHHH 63
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.128 0.379
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,075,559
Number of Sequences: 164201
Number of extensions: 827981
Number of successful extensions: 2506
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2494
Number of HSP's gapped (non-prelim): 22
length of query: 199
length of database: 59,974,054
effective HSP length: 105
effective length of query: 94
effective length of database: 42,732,949
effective search space: 4016897206
effective search space used: 4016897206
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC146332.8