BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC146331.8 + phase: 2 /pseudo
(279 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CDG5_HUMAN (Q9Y5G8) Protocadherin gamma A5 precursor (PCDH-gamma... 35 0.20
CDG2_HUMAN (Q9Y5H1) Protocadherin gamma A2 precursor (PCDH-gamma... 33 0.59
MOAB_BACAN (Q81KI3) Molybdenum cofactor biosynthesis protein B 32 1.7
UVRG_HUMAN (Q9P2Y5) UV radiation resistance-associated gene prot... 32 2.2
TPIS_HELPJ (Q9ZMN8) Triosephosphate isomerase (EC 5.3.1.1) (TIM)... 32 2.2
EDE1_MOUSE (Q925U4) ER degradation-enhancing alpha-mannosidase-like 32 2.2
CDG3_HUMAN (Q9Y5H0) Protocadherin gamma A3 precursor (PCDH-gamma... 32 2.2
PUR5_SHIFL (Q83QL4) Phosphoribosylformylglycinamidine cyclo-liga... 31 2.9
PUR5_ECOLI (P08178) Phosphoribosylformylglycinamidine cyclo-liga... 31 2.9
PUR5_ECOL6 (Q8FF72) Phosphoribosylformylglycinamidine cyclo-liga... 31 2.9
PUR5_ECO57 (Q8XAC5) Phosphoribosylformylglycinamidine cyclo-liga... 31 2.9
MOAB_BACCR (Q816R0) Molybdenum cofactor biosynthesis protein B 31 2.9
TPIS_HELPY (P56076) Triosephosphate isomerase (EC 5.3.1.1) (TIM)... 31 3.8
DNK2_PROMA (Q7V9G2) Chaperone protein dnaK2 (Heat shock protein ... 31 3.8
DRL4_ARATH (Q9SX38) Putative disease resistance protein At1g50180 30 5.0
KIR3_HUMAN (Q8IZU9) Kin of IRRE-like protein 3 precursor (Kin of... 30 6.5
ATKA_LISMO (Q8Y3Z6) Potassium-transporting ATPase A chain (EC 3.... 30 6.5
YLZ9_CAEEL (P34414) Hypothetical protein F42H10.9 in chromosome III 30 8.5
YGT6_YEAST (P53099) Hypothetical 64.5 kDa protein in COX4-GTS1 i... 30 8.5
VG85_BPPH2 (P20344) Head fiber protein (Late protein GP8.5) 30 8.5
>CDG5_HUMAN (Q9Y5G8) Protocadherin gamma A5 precursor
(PCDH-gamma-A5)
Length = 931
Score = 35.0 bits (79), Expect = 0.20
Identities = 27/96 (28%), Positives = 42/96 (43%), Gaps = 13/96 (13%)
Query: 143 AYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKS------EGEVLSELISSVEESGAKY 196
+Y T E + +G + V + P + N T S +G LS +S ++G Y
Sbjct: 456 SYSTSVTENNPRGVSIFSVTAHDPDSGDNARVTYSLAEDTFQGAPLSSYVSINSDTGVLY 515
Query: 197 AVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNS 232
A+ RS Y RDLQ ++ S +GN +S
Sbjct: 516 AL-------RSFDYEQLRDLQLWVTASDSGNPPLSS 544
>CDG2_HUMAN (Q9Y5H1) Protocadherin gamma A2 precursor
(PCDH-gamma-A2)
Length = 932
Score = 33.5 bits (75), Expect = 0.59
Identities = 29/105 (27%), Positives = 45/105 (42%), Gaps = 13/105 (12%)
Query: 134 ETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKS------EGEVLSELIS 187
+ A S +Y T E + +G + V + P ++ N T S +G LS IS
Sbjct: 447 DNAPAFSRTSYSTYIPENNPRGASVFSVTAHDPDSNDNAHVTYSFAEDTVQGAPLSSYIS 506
Query: 188 SVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNS 232
++G YA+ RS Y RDLQ ++ +GN +S
Sbjct: 507 INSDTGVLYAL-------RSFDYEQLRDLQVWVIARDSGNPPLSS 544
>MOAB_BACAN (Q81KI3) Molybdenum cofactor biosynthesis protein B
Length = 169
Score = 32.0 bits (71), Expect = 1.7
Identities = 26/91 (28%), Positives = 37/91 (40%), Gaps = 2/91 (2%)
Query: 173 DSTKSEGEVLSELISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNS 232
+ T G++L EL+ + Y + V D SIQ R + NG T
Sbjct: 27 EETDKSGQLLHELLKEAGHTVTSYEI--VKDDKESIQQAVLAGYHREDVDVVLTNGGTGI 84
Query: 233 TACDGVCQLKSSLLEGLLVGIVLLIILISGL 263
T D + S+LL +VG L +IS L
Sbjct: 85 TKRDVTIEAVSALLHKEIVGFGELFRMISYL 115
>UVRG_HUMAN (Q9P2Y5) UV radiation resistance-associated gene protein
(p63)
Length = 699
Score = 31.6 bits (70), Expect = 2.2
Identities = 23/58 (39%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Query: 126 SMGNGNREETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKSEGEVLS 183
SMG R+ T+ S+ L KE KG+ DLV NG A NV ++ +GE LS
Sbjct: 536 SMGETERKITSLSSSLDTSLDFSKENKKKGE-DLVGSLNGGHA--NVHPSQEQGEALS 590
>TPIS_HELPJ (Q9ZMN8) Triosephosphate isomerase (EC 5.3.1.1) (TIM)
(Triose-phosphate isomerase)
Length = 234
Score = 31.6 bits (70), Expect = 2.2
Identities = 32/133 (24%), Positives = 62/133 (46%), Gaps = 13/133 (9%)
Query: 130 GNREETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKSEGEVLSELISSV 189
G+ E L+ ++L K K + KGK +V+C G + ++ E LSE + ++
Sbjct: 89 GHSERRVLLKESPSFL-KEKFDFFKGKNFKIVYCIGEDLTTREKGFRAVKEFLSEQLENI 147
Query: 190 EESGAKYAVLYV------SDISRSIQ--YPSYRDLQRFLAEST--TGNGSTNSTACDGVC 239
+ + + V Y + S S++ Y ++ L++ L + T GS N+ +
Sbjct: 148 DLNYSNLIVAYEPIWAIGTKKSASLEDIYLTHGFLKQILNQKTPLLYGGSVNAQNAKEIL 207
Query: 240 QLKSSLLEGLLVG 252
+ S ++GLL+G
Sbjct: 208 GIDS--VDGLLIG 218
>EDE1_MOUSE (Q925U4) ER degradation-enhancing alpha-mannosidase-like
Length = 652
Score = 31.6 bits (70), Expect = 2.2
Identities = 21/70 (30%), Positives = 37/70 (52%), Gaps = 5/70 (7%)
Query: 136 AALQSVQAYLTKRKEESHKGKTDLVVFCN-----GPQASKNVDSTKSEGEVLSELISSVE 190
AA QS+Q+YL + +E ++G+ D ++ N G + +DS ++ L LI VE
Sbjct: 389 AAYQSIQSYLRRGREACNEGEGDPPLYVNVNMFSGQLMNTWIDSLQAFFPGLQVLIGDVE 448
Query: 191 ESGAKYAVLY 200
++ +A Y
Sbjct: 449 DAICLHAFYY 458
>CDG3_HUMAN (Q9Y5H0) Protocadherin gamma A3 precursor
(PCDH-gamma-A3)
Length = 932
Score = 31.6 bits (70), Expect = 2.2
Identities = 26/99 (26%), Positives = 42/99 (42%), Gaps = 16/99 (16%)
Query: 140 SVQAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKS------EGEVLSELISSVEESG 193
S AY+ E + +G + V P ++ N T + +G LS +S +G
Sbjct: 456 SYSAYIP---ENNPRGASIFSVTAQDPDSNNNARITYALTEDTLQGAPLSSFVSINSNTG 512
Query: 194 AKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNS 232
YA+ RS Y +RDL+ + S +GN +S
Sbjct: 513 VLYAL-------RSFDYEQFRDLKLLVTASDSGNPPLSS 544
>PUR5_SHIFL (Q83QL4) Phosphoribosylformylglycinamidine cyclo-ligase
(EC 6.3.3.1) (AIRS) (Phosphoribosyl-aminoimidazole
synthetase) (AIR synthase)
Length = 344
Score = 31.2 bits (69), Expect = 2.9
Identities = 30/119 (25%), Positives = 48/119 (40%), Gaps = 25/119 (21%)
Query: 89 YVSASEDVNLEDSLVSGFAEACGDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKR 148
Y + DV+ +++SG AE C CS+ G E + + Y
Sbjct: 108 YATGKLDVDTASAVISGIAEGC----------LQSGCSLVGGETAEMPGMYHGEDY---- 153
Query: 149 KEESHKGKTDLVVFCNG-PQASKNVDSTK-SEGEVLSELISSVEESGAKYAVLYVSDIS 205
D+ FC G + S+ +D +K S+G+VL L SS S V + ++S
Sbjct: 154 ---------DVAGFCVGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVS 203
>PUR5_ECOLI (P08178) Phosphoribosylformylglycinamidine cyclo-ligase
(EC 6.3.3.1) (AIRS) (Phosphoribosyl-aminoimidazole
synthetase) (AIR synthase)
Length = 344
Score = 31.2 bits (69), Expect = 2.9
Identities = 30/119 (25%), Positives = 48/119 (40%), Gaps = 25/119 (21%)
Query: 89 YVSASEDVNLEDSLVSGFAEACGDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKR 148
Y + DV+ +++SG AE C CS+ G E + + Y
Sbjct: 108 YATGKLDVDTASAVISGIAEGC----------LQSGCSLVGGETAEMPGMYHGEDY---- 153
Query: 149 KEESHKGKTDLVVFCNG-PQASKNVDSTK-SEGEVLSELISSVEESGAKYAVLYVSDIS 205
D+ FC G + S+ +D +K S+G+VL L SS S V + ++S
Sbjct: 154 ---------DVAGFCVGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVS 203
>PUR5_ECOL6 (Q8FF72) Phosphoribosylformylglycinamidine cyclo-ligase
(EC 6.3.3.1) (AIRS) (Phosphoribosyl-aminoimidazole
synthetase) (AIR synthase)
Length = 344
Score = 31.2 bits (69), Expect = 2.9
Identities = 30/119 (25%), Positives = 48/119 (40%), Gaps = 25/119 (21%)
Query: 89 YVSASEDVNLEDSLVSGFAEACGDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKR 148
Y + DV+ +++SG AE C CS+ G E + + Y
Sbjct: 108 YATGKLDVDTASAVISGIAEGC----------LQSGCSLVGGETAEMPGMYHGEDY---- 153
Query: 149 KEESHKGKTDLVVFCNG-PQASKNVDSTK-SEGEVLSELISSVEESGAKYAVLYVSDIS 205
D+ FC G + S+ +D +K S+G+VL L SS S V + ++S
Sbjct: 154 ---------DVAGFCVGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVS 203
>PUR5_ECO57 (Q8XAC5) Phosphoribosylformylglycinamidine cyclo-ligase
(EC 6.3.3.1) (AIRS) (Phosphoribosyl-aminoimidazole
synthetase) (AIR synthase)
Length = 344
Score = 31.2 bits (69), Expect = 2.9
Identities = 30/119 (25%), Positives = 48/119 (40%), Gaps = 25/119 (21%)
Query: 89 YVSASEDVNLEDSLVSGFAEACGDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKR 148
Y + DV+ +++SG AE C CS+ G E + + Y
Sbjct: 108 YATGKLDVDTASAVISGIAEGC----------LQSGCSLVGGETAEMPGMYHGEDY---- 153
Query: 149 KEESHKGKTDLVVFCNG-PQASKNVDSTK-SEGEVLSELISSVEESGAKYAVLYVSDIS 205
D+ FC G + S+ +D +K S+G+VL L SS S V + ++S
Sbjct: 154 ---------DVAGFCVGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVS 203
>MOAB_BACCR (Q816R0) Molybdenum cofactor biosynthesis protein B
Length = 169
Score = 31.2 bits (69), Expect = 2.9
Identities = 25/91 (27%), Positives = 37/91 (40%), Gaps = 2/91 (2%)
Query: 173 DSTKSEGEVLSELISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGSTNS 232
+ T G++L EL+ Y + V D SIQ + + NG T
Sbjct: 27 EETDKSGQLLHELLKEAGHKVTSYEI--VKDDKESIQQAVLAGYHKEDVDVVLTNGGTGI 84
Query: 233 TACDGVCQLKSSLLEGLLVGIVLLIILISGL 263
T D + S+LL+ +VG L +IS L
Sbjct: 85 TKRDVTIEAVSALLDKEIVGFGELFRMISYL 115
>TPIS_HELPY (P56076) Triosephosphate isomerase (EC 5.3.1.1) (TIM)
(Triose-phosphate isomerase)
Length = 234
Score = 30.8 bits (68), Expect = 3.8
Identities = 32/133 (24%), Positives = 61/133 (45%), Gaps = 13/133 (9%)
Query: 130 GNREETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQASKNVDSTKSEGEVLSELISSV 189
G+ E L+ ++L K K + K K +V+C G + + K+ E LSE + ++
Sbjct: 89 GHSERRTLLKESPSFL-KEKFDFFKSKNFKIVYCIGEELTTREKGFKAVKEFLSEQLENI 147
Query: 190 EESGAKYAVLYV------SDISRSIQ--YPSYRDLQRFLAEST--TGNGSTNSTACDGVC 239
+ + V Y + S S++ Y ++ L++ L + T GS N+ +
Sbjct: 148 DLNYPNLVVAYEPIWAIGTKKSASLEDIYLTHGFLKQILNQKTPLLYGGSVNTQNAKEIL 207
Query: 240 QLKSSLLEGLLVG 252
+ S ++GLL+G
Sbjct: 208 GIDS--VDGLLIG 218
>DNK2_PROMA (Q7V9G2) Chaperone protein dnaK2 (Heat shock protein
70-2) (Heat shock 70 kDa protein 2) (HSP70-2)
Length = 633
Score = 30.8 bits (68), Expect = 3.8
Identities = 43/186 (23%), Positives = 76/186 (40%), Gaps = 22/186 (11%)
Query: 104 SGFAEACGDDLG---IGNVAFLGSCSMGNGNREETAALQSVQAYLTKRKEE---SHKGKT 157
SG GDD + ++A + G R++ ALQ + K K E + + +
Sbjct: 216 SGDTHLGGDDFDKVIVDHLAATFKANEGIDLRQDKQALQRLTEAAEKAKIELSNATQSEI 275
Query: 158 DLVVFCNGPQASKNVDSTKSEG---EVLSELISSVE---ESGAKYAVLYVSDISR----- 206
+L P+ K+VD T + E+ S LI E K A L +I
Sbjct: 276 NLPFITATPEGPKHVDLTLTRAKFEELASNLIDRCRVPVEQALKDAKLSTGEIDEIVMVG 335
Query: 207 -SIQYPSYRDLQRFLAESTTGNGSTNSTACDGVCQLKSSLLEGLLVGIVLLIILISGLCC 265
S + P+ ++L + TG + D V + +++ G+L G V I+L+
Sbjct: 336 GSTRMPAVKEL----VKRVTGKDPNQTVNPDEVVAVGAAIQGGVLAGEVKDILLLDVTPL 391
Query: 266 MMGIDS 271
+G+++
Sbjct: 392 SLGVET 397
>DRL4_ARATH (Q9SX38) Putative disease resistance protein At1g50180
Length = 839
Score = 30.4 bits (67), Expect = 5.0
Identities = 34/114 (29%), Positives = 49/114 (42%), Gaps = 17/114 (14%)
Query: 46 LALLFVGGELQSSDLSLNKHADSALSYLLKDSFVRSNTSM---------AFPYVSASEDV 96
++L VG E++ L+K A S L + +K+S R S+ +FPYV V
Sbjct: 106 VSLHSVGSEIREITSRLSKIAASMLDFGIKESMGREGLSLSDSLREQRQSFPYVVEHNLV 165
Query: 97 NLEDSLVSGFAEACGDDLGIG--NVAFLGSCSMGNGNREETAALQSVQAYLTKR 148
LE SL E +DL G + C MG G + T A Q + +R
Sbjct: 166 GLEQSL-----EKLVNDLVSGGEKLRVTSICGMG-GLGKTTLAKQIFHHHKVRR 213
>KIR3_HUMAN (Q8IZU9) Kin of IRRE-like protein 3 precursor (Kin of
irregular chiasm-like protein 3) (Nephrin-like 2)
(UNQ5923/PRO19814)
Length = 778
Score = 30.0 bits (66), Expect = 6.5
Identities = 19/54 (35%), Positives = 24/54 (44%), Gaps = 1/54 (1%)
Query: 43 PLDLALLFVGGELQSSDLSLNKHADS-ALSYLLKDSFVRSNTSMAFPYVSASED 95
P L LLFV L S +L L K L Y+ KD F R N + + +D
Sbjct: 3 PFQLDLLFVCFFLFSQELGLQKRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQD 56
>ATKA_LISMO (Q8Y3Z6) Potassium-transporting ATPase A chain (EC
3.6.3.12) (Potassium-translocating ATPase A chain) (ATP
phosphohydrolase [potassium-transporting] A chain)
(Potassium binding and translocating subunit A)
Length = 561
Score = 30.0 bits (66), Expect = 6.5
Identities = 29/115 (25%), Positives = 45/115 (38%), Gaps = 10/115 (8%)
Query: 157 TDLVVFCNGPQASKNVDSTKSEGEVLSELISSVEESGAKYAVLYVSDISRSIQYPSYRDL 216
T + V QAS + EVL S +G+ +A ++ L
Sbjct: 433 TAVAVMMPSVQASVSASGAHGFSEVLYAFTSMGNNNGSAFAGFAADTTFTNMVGAVMMLL 492
Query: 217 QRF--------LAESTTGNGSTNSTACDGVCQLKSSLLEGLLVGIVLLIILISGL 263
RF LA++ G S A G K+ + GLL+G+V+L+ +S L
Sbjct: 493 ARFIPLVAALYLAQNMAGKSSV--AASSGTLSTKNGMFIGLLIGVVVLVGALSFL 545
>YLZ9_CAEEL (P34414) Hypothetical protein F42H10.9 in chromosome III
Length = 309
Score = 29.6 bits (65), Expect = 8.5
Identities = 15/35 (42%), Positives = 18/35 (50%)
Query: 204 ISRSIQYPSYRDLQRFLAESTTGNGSTNSTACDGV 238
+S S P Y L A TGNGSTN+ A G+
Sbjct: 238 VSSSSTLPKYDQLYCNTASPLTGNGSTNTIATTGI 272
>YGT6_YEAST (P53099) Hypothetical 64.5 kDa protein in COX4-GTS1
intergenic region
Length = 579
Score = 29.6 bits (65), Expect = 8.5
Identities = 36/142 (25%), Positives = 58/142 (40%), Gaps = 13/142 (9%)
Query: 146 TKRKEESHKGKTDLVVFCNGPQASKNV----DSTKSEGEVLSELISSVEESGAKYAVLYV 201
TKRKE+ K TD++ F A+ ++ + S +L +I+ K + V
Sbjct: 9 TKRKEDHTKHTTDVIEFYEEGTAASSLNIATEKANSSPSILRRIINRAAWLSKKVDAMGV 68
Query: 202 --SDISRSIQYPSYRDLQRFLAE-----STTGNGSTNSTACDGVCQLKSSLLEGLLVGIV 254
+ I R Y ++FL S TG S+ S+ G S E + ++
Sbjct: 69 ESTGIQRISPYERGTSKKQFLHVAGLWLSATGGLSSMSSFLLGPLLFGLSFRESVASSLI 128
Query: 255 LLII--LISGLCCMMGIDSPTR 274
+ I LI+ C +MG S R
Sbjct: 129 SVTIGCLIAAYCSIMGPQSGCR 150
>VG85_BPPH2 (P20344) Head fiber protein (Late protein GP8.5)
Length = 280
Score = 29.6 bits (65), Expect = 8.5
Identities = 32/152 (21%), Positives = 59/152 (38%), Gaps = 7/152 (4%)
Query: 111 GDDLGIGNVAFLGSCSMGNGNREETAALQSVQAYLTKRKEESHKGKTDLVVFCNGPQASK 170
G D+ +G+ G +G T +V Y EE+ +G+ +V + S
Sbjct: 68 GSDIKVGDNLTTGK----DGTAVPTDDPSTVFGYAV---EEAQEGQLVTLVISRSKEISI 120
Query: 171 NVDSTKSEGEVLSELISSVEESGAKYAVLYVSDISRSIQYPSYRDLQRFLAESTTGNGST 230
VD K G+ L+ SGA+ ++ D I + ++ L + +G+
Sbjct: 121 EVDDIKDAGDTGKRLLKINTPSGARNIIIENEDAKALINGETTNTNKKNLQDLLFSDGNV 180
Query: 231 NSTACDGVCQLKSSLLEGLLVGIVLLIILISG 262
+ + L+ LLV ++ L+SG
Sbjct: 181 KAFLQATTTDENKTALQQLLVSNADVLGLLSG 212
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.313 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,543,345
Number of Sequences: 164201
Number of extensions: 1133808
Number of successful extensions: 2640
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 2638
Number of HSP's gapped (non-prelim): 25
length of query: 279
length of database: 59,974,054
effective HSP length: 109
effective length of query: 170
effective length of database: 42,076,145
effective search space: 7152944650
effective search space used: 7152944650
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 65 (29.6 bits)
Medicago: description of AC146331.8