BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC146329.11 + phase: 0 
         (327 letters)

Database: sprot 
           164,201 sequences; 59,974,054 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

NFHT_CAEEL (O76463) Nitrilase and fragile histidine triad fusion...   260  3e-69
NIT1_HUMAN (Q86X76) Nitrilase homolog 1 (EC 3.5.-.-)                  257  3e-68
NIT1_RAT (Q7TQ94) Nitrilase homolog 1 (EC 3.5.-.-)                    253  4e-67
NIT1_MOUSE (Q8VDK1) Nitrilase homolog 1 (EC 3.5.-.-)                  253  4e-67
NFHT_DROME (O76464) Nitrilase and fragile histidine triad fusion...   215  1e-55
Y601_SYNY3 (P55175) Hypothetical UPF0012 protein sll0601 (EC 3.5...   182  1e-45
NIT2_YEAST (P47016) Probable hydrolase NIT2 (EC 3.5.-.-)              179  1e-44
YAUB_SCHPO (Q10166) Hypothetical UPF0012 protein C26A3.11 in chr...   165  1e-40
NIT3_YEAST (P49954) Probable hydrolase NIT3 (EC 3.5.-.-)              147  4e-35
Y480_MYCTU (Q11146) Hypothetical UPF0012 protein Rv0480c/MT0498 ...   112  1e-24
YBEM_ECOLI (P39874) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)    108  2e-23
YBEM_ECO57 (P58054) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)    106  9e-23
YPQQ_PSEFL (P55176) Hypothetical UPF0012 protein in pqqF 5'regio...    86  1e-16
YAG5_STALU (P55178) Hypothetical UPF0012 protein in agr operon (...    84  5e-16
YAG5_STAAU (P55177) Hypothetical UPF0012 protein in agr operon (...    80  5e-15
NRL1_ARATH (P32961) Nitrilase 1 (EC 3.5.5.1)                           80  5e-15
NRL2_ARATH (P32962) Nitrilase 2 (EC 3.5.5.1)                           79  2e-14
NRL3_ARATH (P46010) Nitrilase 3 (EC 3.5.5.1)                           78  3e-14
NRL4_TOBAC (Q42965) Nitrilase 4 (EC 3.5.5.1)                           74  7e-13
BUP1_HUMAN (Q9UBR1) Beta-ureidopropionase (EC 3.5.1.6) (Beta-ala...    70  6e-12

>NFHT_CAEEL (O76463) Nitrilase and fragile histidine triad fusion
           protein NitFhit [Includes:
           Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
           (Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
           (Dinucleosidetriphosphatase) (AP3A hydrolase) (AP3AASE);
           Nitrilas
          Length = 440

 Score =  260 bits (665), Expect = 3e-69
 Identities = 130/276 (47%), Positives = 180/276 (65%), Gaps = 5/276 (1%)

Query: 33  TTGESTMATNSVRVAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKD 92
           T    TMAT    +A  QMTS  DL  NF     +++ A     +++  PE F F+G   
Sbjct: 4   TVFRRTMATGRHFIAVCQMTSDNDLEKNFQAAKNMIERAGEKKCEMVFLPECFDFIGLNK 63

Query: 93  GDSVSIAQPLDGPIMDQYCSLARESSIWLSLGGFQEKG-SDPRHLFNTHVVVDDTGKIQT 151
            + + +A   D   M++Y  LAR+ +IWLSLGG   K  SD  H +NTH+++D  G  + 
Sbjct: 64  NEQIDLAMATDCEYMEKYRELARKHNIWLSLGGLHHKDPSDAAHPWNTHLIIDSDGVTRA 123

Query: 152 TYRKIHLFDVDVPGGRVYKESNFTESGKDIVA-VDSPIGRLGLSVCYDLRFPELYQLLRF 210
            Y K+HLFD+++PG     ES F+++G +++  VD+PIGRLGLS+CYD+RFPEL  L   
Sbjct: 124 EYNKLHLFDLEIPGKVRLMESEFSKAGTEMIPPVDTPIGRLGLSICYDVRFPEL-SLWNR 182

Query: 211 QHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIID 270
           + GAQ+L  P+AFT  TG AHWE LLRARAIENQCYV+AAAQ G HN KR+SYG ++++D
Sbjct: 183 KRGAQLLSFPSAFTLNTGLAHWETLLRARAIENQCYVVAAAQTGAHNPKRQSYGHSMVVD 242

Query: 271 PWGTVVGRLPDRLSTGIVVADIDLSLVDSVREKMPI 306
           PWG VV +  +R+   +  A+IDLS VD++RE  P+
Sbjct: 243 PWGAVVAQCSERVD--MCFAEIDLSYVDTLREMQPV 276


>NIT1_HUMAN (Q86X76) Nitrilase homolog 1 (EC 3.5.-.-)
          Length = 327

 Score =  257 bits (656), Expect = 3e-68
 Identities = 127/265 (47%), Positives = 178/265 (66%), Gaps = 7/265 (2%)

Query: 46  VAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSVSIAQPLDGP 105
           VA  Q+TS  D   NF TC+ LV+EAA  GA L   PEAF F+     +++ +++PL G 
Sbjct: 49  VAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGK 108

Query: 106 IMDQYCSLARESSIWLSLGGFQEKGSD---PRHLFNTHVVVDDTGKIQTTYRKIHLFDVD 162
           ++++Y  LARE  +WLSLGGF E+G D    + ++N HV+++  G +  TYRK HL DV+
Sbjct: 109 LLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGAVVATYRKTHLCDVE 168

Query: 163 VPGGRVYKESNFTESGKDIVA-VDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPA 221
           +PG     ESN T  G  + + V +P G++GL+VCYD+RFPEL  L   Q GA+IL  P+
Sbjct: 169 IPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPEL-SLALAQAGAEILTYPS 227

Query: 222 AFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPD 281
           AF  +TG AHWE+LLRARAIE QCYV+AAAQ G H++KR SYG ++++DPWGTVV R  +
Sbjct: 228 AFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSE 287

Query: 282 RLSTGIVVADIDLSLVDSVREKMPI 306
               G+ +A IDL+ +  +R  +P+
Sbjct: 288 --GPGLCLARIDLNYLRQLRRHLPV 310


>NIT1_RAT (Q7TQ94) Nitrilase homolog 1 (EC 3.5.-.-)
          Length = 292

 Score =  253 bits (647), Expect = 4e-67
 Identities = 126/265 (47%), Positives = 177/265 (66%), Gaps = 7/265 (2%)

Query: 46  VAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSVSIAQPLDGP 105
           VA  Q+TS  +   NF TC+ LV+EA   GA L   PEAF F+     +++ +++PLDG 
Sbjct: 14  VAVCQVTSTPNKQENFKTCAELVQEATRLGACLAFLPEAFDFIARNPAETLLLSEPLDGD 73

Query: 106 IMDQYCSLARESSIWLSLGGFQEKGSD---PRHLFNTHVVVDDTGKIQTTYRKIHLFDVD 162
           ++ QY  LARE  IWLSLGGF E+G D    + ++N HV+++  G +  +YRK HL DV+
Sbjct: 74  LLGQYSQLARECGIWLSLGGFHERGQDWEQTQKIYNCHVLLNSKGSVVASYRKTHLCDVE 133

Query: 163 VPGGRVYKESNFTESGKDIVA-VDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPA 221
           +PG    +ESN+T  G  +   V +P G++GL++CYD+RFPEL  L   Q GA+IL  P+
Sbjct: 134 IPGQGPMRESNYTMPGYALEPPVKTPAGKVGLAICYDMRFPEL-SLKLAQAGAEILTYPS 192

Query: 222 AFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPD 281
           AF  VTG AHWE+LLRARAIE+QCYVIAAAQ G H++ R SYG ++++DPWGTVV    +
Sbjct: 193 AFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGTVVASCSE 252

Query: 282 RLSTGIVVADIDLSLVDSVREKMPI 306
               G+ +A IDL  +  +R+ +P+
Sbjct: 253 --GPGLCLARIDLHFLQQMRQHLPV 275


>NIT1_MOUSE (Q8VDK1) Nitrilase homolog 1 (EC 3.5.-.-)
          Length = 323

 Score =  253 bits (647), Expect = 4e-67
 Identities = 126/265 (47%), Positives = 179/265 (67%), Gaps = 7/265 (2%)

Query: 46  VAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSVSIAQPLDGP 105
           VA  Q+TS  +   NF TC+ LV+EAA  GA L   PEAF F+     +++ +++PL+G 
Sbjct: 45  VAVCQVTSTPNKQENFKTCAELVQEAARLGACLAFLPEAFDFIARNPAETLLLSEPLNGD 104

Query: 106 IMDQYCSLARESSIWLSLGGFQEKGSD---PRHLFNTHVVVDDTGKIQTTYRKIHLFDVD 162
           ++ QY  LARE  IWLSLGGF E+G D    + ++N HV+++  G +  +YRK HL DV+
Sbjct: 105 LLGQYSQLARECGIWLSLGGFHERGQDWEQNQKIYNCHVLLNSKGSVVASYRKTHLCDVE 164

Query: 163 VPGGRVYKESNFTESGKDIVA-VDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPA 221
           +PG    +ESN+T+ G  +   V +P G++GL++CYD+RFPEL  L   Q GA+IL   +
Sbjct: 165 IPGQGPMRESNYTKPGGTLEPPVKTPAGKVGLAICYDMRFPEL-SLKLAQAGAEILTYSS 223

Query: 222 AFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPD 281
           AF  VTG AHWE+LLRARAIE+QCYVIAAAQ G H++ R SYG ++++DPWGTVV R  +
Sbjct: 224 AFGSVTGPAHWEVLLRARAIESQCYVIAAAQCGRHHETRASYGHSMVVDPWGTVVARCSE 283

Query: 282 RLSTGIVVADIDLSLVDSVREKMPI 306
               G+ +A IDL  +  +R+ +P+
Sbjct: 284 --GPGLCLARIDLHFLQQMRQHLPV 306


>NFHT_DROME (O76464) Nitrilase and fragile histidine triad fusion
           protein NitFhit (NFT-1 protein) [Includes:
           Bis(5'-adenosyl)-triphosphatase (EC 3.6.1.29)
           (Diadenosine 5',5'''-P1,P3-triphosphate hydrolase)
           (Dinucleosidetriphosphatase) (AP3A hydrolase) (AP
          Length = 460

 Score =  215 bits (548), Expect = 1e-55
 Identities = 113/263 (42%), Positives = 164/263 (61%), Gaps = 4/263 (1%)

Query: 43  SVRVAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSVSIAQPL 102
           S  +A  QM S +D A+N S    LV  A S  A +L  PE   FVG     ++ +++ L
Sbjct: 32  SATIAVGQMRSTSDKAANLSQVIELVDRAKSQNACMLFLPECCDFVGESRTQTIELSEGL 91

Query: 103 DGPIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHVVVDDTGKIQTTYRKIHLFDVD 162
           DG +M QY  LA+ + IW+SLGG  E+    + +FN HV++++ G++   YRK+H+FDV 
Sbjct: 92  DGELMAQYRELAKCNKIWISLGGVHERND--QKIFNAHVLLNEKGELAAVYRKLHMFDVT 149

Query: 163 VPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPAA 222
               R+ +    T        V +P+G++GL +CYDLRF E   LLR + GA +L  P+A
Sbjct: 150 TKEVRLRESDTVTPGYCLERPVSTPVGQIGLQICYDLRFAEPAVLLR-KLGANLLTYPSA 208

Query: 223 FTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPDR 282
           FT  TG+AHWEILLRARAIE QC+V+AAAQ G HN KR+S+G ++I+ PWG V+    ++
Sbjct: 209 FTYATGKAHWEILLRARAIETQCFVVAAAQIGWHNQKRQSWGHSMIVSPWGNVLADCSEQ 268

Query: 283 LSTGIVVADIDLSLVDSVREKMP 305
               I  A++DLS++ S+ + MP
Sbjct: 269 -ELDIGTAEVDLSVLQSLYQTMP 290


>Y601_SYNY3 (P55175) Hypothetical UPF0012 protein sll0601 (EC
           3.5.-.-)
          Length = 272

 Score =  182 bits (461), Expect = 1e-45
 Identities = 102/262 (38%), Positives = 150/262 (56%), Gaps = 7/262 (2%)

Query: 47  AAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSVSIAQPLDGPI 106
           AA QMTS  +L  N      L+  A   GA+L+  PE F+F+G  + + +  A  +    
Sbjct: 7   AALQMTSRPNLTENLQEAEELIDLAVRQGAELVGLPENFAFLG-NETEKLEQATAIATAT 65

Query: 107 MDQYCSLARESSIWLSLGGFQ-EKGSDPRHLFNTHVVVDDTGKIQTTYRKIHLFDVDVPG 165
                ++A+   + +  GGF      +    +NT  ++   G+    Y K+HLFDV+VP 
Sbjct: 66  EKFLQTMAQRFQVTILAGGFPFPVAGEAGKAYNTATLIAPNGQELARYHKVHLFDVNVPD 125

Query: 166 GRVYKESNFTESGKDIVAV--DSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPAAF 223
           G  Y ES    +G+    V      G LGLS+CYD+RFPELY+ L  Q GA +L VPAAF
Sbjct: 126 GNTYWESATVMAGQKYPPVYHSDSFGNLGLSICYDVRFPELYRYLSRQ-GADVLFVPAAF 184

Query: 224 TKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPDRL 283
           T  TG+ HW++LL+ARAIEN CYVIA AQ G H ++R ++G  +IIDPWG ++    ++ 
Sbjct: 185 TAYTGKDHWQVLLQARAIENTCYVIAPAQTGCHYERRHTHGHAMIIDPWGVILADAGEK- 243

Query: 284 STGIVVADIDLSLVDSVREKMP 305
             G+ +A+I+   +  VR++MP
Sbjct: 244 -PGLAIAEINPDRLKQVRQQMP 264


>NIT2_YEAST (P47016) Probable hydrolase NIT2 (EC 3.5.-.-)
          Length = 307

 Score =  179 bits (453), Expect = 1e-44
 Identities = 110/298 (36%), Positives = 159/298 (52%), Gaps = 31/298 (10%)

Query: 39  MATNSVRVAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSVSI 98
           M +   RVA AQ+ S  DL  N      L+ EA    A ++  PEA  ++      S  +
Sbjct: 1   MTSKLKRVAVAQLCSSADLTKNLKVVKELISEAIQKKADVVFLPEASDYLSQNPLHSRYL 60

Query: 99  AQPLDGPIMDQYCS---LARESS--IWLSLG-----GFQEKGSDPRHLFNTHVVVDDTGK 148
           AQ     I     S   L R++S  I +S+G       Q+       + N  + +D  GK
Sbjct: 61  AQKSPKFIRQLQSSITDLVRDNSRNIDVSIGVHLPPSEQDLLEGNDRVRNVLLYIDHEGK 120

Query: 149 IQTTYRKIHLFDVDVPGGRVYKESNFTESGKDIV-AVDSPIGRLGLSVCYDLRFPELYQL 207
           I   Y+K+HLFDVDVP G + KES   + GK I   ++SP+G+LG ++CYD+RFPE    
Sbjct: 121 ILQEYQKLHLFDVDVPNGPILKESKSVQPGKAIPDIIESPLGKLGSAICYDIRFPEFSLK 180

Query: 208 LRFQHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTH----------- 256
           LR   GA+IL  P+AFT  TGEAHWE+L RARA++ QCYV+   Q G H           
Sbjct: 181 LRSM-GAEILCFPSAFTIKTGEAHWELLGRARAVDTQCYVLMPGQVGMHDLSDPEWEKQS 239

Query: 257 -------NDKRESYGDTLIIDPWGTVVGRL-PDRLSTGIVVADIDLSLVDSVREKMPI 306
                  + +RES+G +++IDPWG ++    P  +   +++AD+D  L+  +R KMP+
Sbjct: 240 HMSALEKSSRRESWGHSMVIDPWGKIIAHADPSTVGPQLILADLDRELLQEIRNKMPL 297


>YAUB_SCHPO (Q10166) Hypothetical UPF0012 protein C26A3.11 in
           chromosome I (EC 3.5.-.-)
          Length = 322

 Score =  165 bits (418), Expect = 1e-40
 Identities = 91/264 (34%), Positives = 147/264 (55%), Gaps = 8/264 (3%)

Query: 45  RVAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSVSIAQPLD- 103
           R+   Q+ +  D + N       V EAA  G+ ++  PE F+      G     A+P++ 
Sbjct: 45  RIGLVQLANTKDKSENLQLARLKVLEAAKNGSNVIVLPEIFNSPYGT-GYFNQYAEPIEE 103

Query: 104 -GPIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHVVVDDTGKIQTTYRKIHLFDVD 162
             P      S+A+++  +L  G   E+      L+NT +V D +GK+   +RKIHLFD+D
Sbjct: 104 SSPSYQALSSMAKDTKTYLFGGSIPERKDGK--LYNTAMVFDPSGKLIAVHRKIHLFDID 161

Query: 163 VPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPAA 222
           +PGG  ++ES+    G  +  VD+  G+ GL +CYD+RFPEL  ++  ++G  +++ P A
Sbjct: 162 IPGGVSFRESDSLSPGDAMTMVDTEYGKFGLGICYDIRFPEL-AMIAARNGCSVMIYPGA 220

Query: 223 FTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPDR 282
           F   TG  HWE+L RARA++N+ +V   A A   N    S+G + ++DP+G V+    ++
Sbjct: 221 FNLSTGPLHWELLARARAVDNEMFVACCAPARDMNADYHSWGHSTVVDPFGKVIATTDEK 280

Query: 283 LSTGIVVADIDLSLVDSVREKMPI 306
            S  IV ADID S++ + R  +PI
Sbjct: 281 PS--IVYADIDPSVMSTARNSVPI 302


>NIT3_YEAST (P49954) Probable hydrolase NIT3 (EC 3.5.-.-)
          Length = 291

 Score =  147 bits (371), Expect = 4e-35
 Identities = 86/285 (30%), Positives = 149/285 (52%), Gaps = 9/285 (3%)

Query: 37  STMATNSVRVAAAQMT-SITDLASNFSTCSRLVKEAASA--GAKLLCFPEAFSFVGAKDG 93
           S + +  ++VA  Q++ S  D  +N    +  ++ A       KL+  PE F+   + D 
Sbjct: 4   SKILSQKIKVALVQLSGSSPDKMANLQRAATFIERAMKEQPDTKLVVLPECFNSPYSTDQ 63

Query: 94  --DSVSIAQPLDGPIMDQYCS-LARESSIWLSLGGFQEKGSDPRHLFNTHVVVDDTGKIQ 150
                 +  P +     Q+ S LA +  I L  G   E       ++NT ++ ++ GK+ 
Sbjct: 64  FRKYSEVINPKEPSTSVQFLSNLANKFKIILVGGTIPELDPKTDKIYNTSIIFNEDGKLI 123

Query: 151 TTYRKIHLFDVDVPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLRFPELYQLLRF 210
             +RK+HLFDVD+P G  + ES     G+    +D+  G+ G+ +CYD+RFPEL  +L  
Sbjct: 124 DKHRKVHLFDVDIPNGISFHESETLSPGEKSTTIDTKYGKFGVGICYDMRFPEL-AMLSA 182

Query: 211 QHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIID 270
           + GA  ++ P+AF  VTG  HW +L R+RA++NQ YV+  + A        +YG ++++D
Sbjct: 183 RKGAFAMIYPSAFNTVTGPLHWHLLARSRAVDNQVYVMLCSPARNLQSSYHAYGHSIVVD 242

Query: 271 PWGTVVGRLPDRLSTGIVVADIDLSLVDSVREKMPIAKVIVCNVY 315
           P G +V    +     I+ A++D  +++S R+ +P+ K    +VY
Sbjct: 243 PRGKIVAEAGE--GEEIIYAELDPEVIESFRQAVPLTKQRRFDVY 285


>Y480_MYCTU (Q11146) Hypothetical UPF0012 protein Rv0480c/MT0498 (EC
           3.5.-.-)
          Length = 340

 Score =  112 bits (281), Expect = 1e-24
 Identities = 92/281 (32%), Positives = 141/281 (49%), Gaps = 32/281 (11%)

Query: 44  VRVAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGDSV-SIAQPL 102
           +R+A AQ+ S TD A+N     +   EAA+AGA+L+ FPEA      + G  +  +A+P+
Sbjct: 61  MRIALAQIRSGTDPAANLQLVGKYAGEAATAGAQLVVFPEA---TMCRLGVPLRQVAEPV 117

Query: 103 DGPIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHVVV--DDTGKIQTTYRKIHLFD 160
           DGP  +    +A E+ I +  G F   G     + NT +        +    Y KIHL+D
Sbjct: 118 DGPWANGVRRIATEAGITVIAGMFTPTGDG--RVTNTLIAAGPGTPNQPDAHYHKIHLYD 175

Query: 161 VDVPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVP 220
                   + ES     G++ V V     R+GL+VCYD+RFP LY  L  + GAQ++ V 
Sbjct: 176 -----AFGFTESRTVAPGREPVVVVVDGVRVGLTVCYDIRFPALYTELA-RRGAQLIAVC 229

Query: 221 AAFTKVTGE-AHWEILLRARAIENQCYVIAAAQA---------GTHNDKRESYGDTLIID 270
           A++    G+   W +L RARA+++  YV AA QA         G  +      G +L+  
Sbjct: 230 ASWGSGPGKLEQWTLLARARALDSMSYVAAAGQADPGDARTGVGASSAAPTGVGGSLVAS 289

Query: 271 PWGTVV---GRLPDRLSTGIVVADIDLSLVDSVREKMPIAK 308
           P G VV   G  P      ++VADID+  V + R+++ + +
Sbjct: 290 PLGEVVVSAGTQPQ-----LLVADIDVDNVAAARDRIAVLR 325


>YBEM_ECOLI (P39874) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)
          Length = 262

 Score =  108 bits (269), Expect = 2e-23
 Identities = 83/264 (31%), Positives = 128/264 (48%), Gaps = 19/264 (7%)

Query: 46  VAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGD-SVSIAQPLDG 104
           VAA Q    +    N   C+ L+ +AA   A L   PEA       D D SV  AQ L+G
Sbjct: 3   VAAGQFAVTSVWEKNAEICASLMAQAAENDASLFALPEALLARDDHDADLSVKSAQLLEG 62

Query: 105 PIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHVVVDDTGKIQTTYRKIHLFDVDVP 164
             + +    ++ + +   L       S P   +N  V +   G I   Y K+HL+D    
Sbjct: 63  EFLGRLRRESKRNMMTTILT--IHVPSTPGRAWNMLVALQ-AGNIVARYAKLHLYDAFA- 118

Query: 165 GGRVYKESNFTESGKDIVAVDSPIG-RLGLSVCYDLRFPELYQLLRFQHGAQILLVPAAF 223
                +ES   ++G +I  +    G ++GL  CYDLRFPEL  L +   GA+IL++PAA+
Sbjct: 119 ----IQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL-ALAQALQGAEILVLPAAW 173

Query: 224 TK-VTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPDR 282
            +    E HW  LL ARA++  CY++AA + G  N      G + IIDP+G  +    + 
Sbjct: 174 VRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKN-----IGQSRIIDPFGVTIAAASE- 227

Query: 283 LSTGIVVADIDLSLVDSVREKMPI 306
               +++A++    V  VR ++P+
Sbjct: 228 -MPALIMAEVTPERVRQVRAQLPV 250


>YBEM_ECO57 (P58054) Hypothetical UPF0012 protein ybeM (EC 3.5.-.-)
          Length = 262

 Score =  106 bits (264), Expect = 9e-23
 Identities = 82/264 (31%), Positives = 127/264 (48%), Gaps = 19/264 (7%)

Query: 46  VAAAQMTSITDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFVGAKDGD-SVSIAQPLDG 104
           VAA Q    +    N   C+ L+ +AA     L   PEA       D D SV  AQ L+G
Sbjct: 3   VAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLARDDHDADLSVKSAQLLEG 62

Query: 105 PIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHVVVDDTGKIQTTYRKIHLFDVDVP 164
             + +    ++ + +   L       S P   +N  V +   G I   Y K+HL+D    
Sbjct: 63  EFLGRLRRESKRNMMTTILT--IHVPSTPGRAWNMLVALQ-AGNIVARYAKLHLYDAFA- 118

Query: 165 GGRVYKESNFTESGKDIVAVDSPIG-RLGLSVCYDLRFPELYQLLRFQHGAQILLVPAAF 223
                +ES   ++G +I  +    G ++GL  CYDLRFPEL  L +   GA+IL++PAA+
Sbjct: 119 ----IQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPEL-ALAQALQGAEILVLPAAW 173

Query: 224 TK-VTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPDR 282
            +    E HW  LL ARA++  CY++AA + G  N      G + IIDP+G  +    + 
Sbjct: 174 VRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKN-----IGQSRIIDPFGVTIAAASE- 227

Query: 283 LSTGIVVADIDLSLVDSVREKMPI 306
               +++A++    V  VR ++P+
Sbjct: 228 -MPALIMAEVTPERVRQVRAQLPV 250


>YPQQ_PSEFL (P55176) Hypothetical UPF0012 protein in pqqF 5'region
           (EC 3.5.-.-) (ORF2)
          Length = 285

 Score = 86.3 bits (212), Expect = 1e-16
 Identities = 80/278 (28%), Positives = 129/278 (45%), Gaps = 22/278 (7%)

Query: 28  ASLSVTTGESTMATNSVRVAAAQMTSIT-DLASNFSTCSRLVKEAASAGAKLLCFPEAFS 86
           A+ +   G S     ++RVA  Q      D+A N     ++  EA  A   LL  PE F 
Sbjct: 6   ATAATVAGLSVSGVKTMRVALYQCPPRPLDVAGNLQRLHQVAMEATDAD--LLVLPEMF- 62

Query: 87  FVGAKDGDSV--SIAQPLDGPIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHVVVD 144
             G   G     ++A+  DGP   +  ++A+ +   + L G+ E+  D + ++N   ++D
Sbjct: 63  LSGYNIGLEAVGALAEAQDGPSAQRIAAIAQAAGTAI-LYGYPERSVDGQ-IYNAVQLID 120

Query: 145 DTGKIQTTYRKIHLF-DVDVPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLRFPE 203
             G+    YRK HLF D+D         S F+    D   V+    +LG  +CYD+ FPE
Sbjct: 121 AQGQRLCNYRKTHLFGDLD--------HSMFSAGEDDFPLVELDGWKLGFLICYDIEFPE 172

Query: 204 LYQLLRFQHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESY 263
             + L    GA+++LVP A   +  +   ++ +RARA ENQCYV  A   G H ++    
Sbjct: 173 NARRLALA-GAELILVPTA-NMIPYDFVADVTIRARAFENQCYVAYANYCG-HEEQIRYC 229

Query: 264 GDTLIIDPWGTVVGRLPDRLSTGIVVADIDLSLVDSVR 301
           G + I  P G+ +      L   +++  +D  L+   R
Sbjct: 230 GQSSIAAPDGSRIALA--GLDEALIIGTLDRQLMGESR 265


>YAG5_STALU (P55178) Hypothetical UPF0012 protein in agr operon (ORF
           5) (Fragment)
          Length = 234

 Score = 84.0 bits (206), Expect = 5e-16
 Identities = 67/208 (32%), Positives = 104/208 (49%), Gaps = 20/208 (9%)

Query: 112 SLARESSIWLSLGGFQEKGSDPRHLFNTHVVVDDTGKIQTTYRKIHLFDVDVPGGRVYKE 171
           +LA +  + +  G    K  D  H+FNT   +D TGK+   Y K+HL    VP   +  E
Sbjct: 42  NLALQYQVDIIAGSVSNKHHD--HIFNTAFAIDKTGKVINQYDKMHL----VP---MLDE 92

Query: 172 SNFTESGKDIVAVDSPIGRLGLS--VCYDLRFPELYQLLRF--QHGAQILLVPAAFTKVT 227
             F  +GK++         + ++  +CYDLRFPEL   LR+  + GA I    A +    
Sbjct: 93  PAFLTAGKNVPETFKLSNGVKVTQMICYDLRFPEL---LRYPARSGATIAFYVAQWPSAR 149

Query: 228 GEAHWEILLRARAIENQCYVIAAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPDRLSTGI 287
              HW++LL+ARAIEN  YVI     G ++ K +  G ++ I+P G ++  L        
Sbjct: 150 LN-HWQVLLKARAIENNMYVIGCNGCG-YDGKTQYAGHSVAINPNGEIIQELSTTEKELT 207

Query: 288 VVADIDLSLVDSVREKMPIAKVIVCNVY 315
           V  DID   V+  R+ +P+   +V ++Y
Sbjct: 208 VTIDID--AVEQQRKAIPVFDSLVPHLY 233


>YAG5_STAAU (P55177) Hypothetical UPF0012 protein in agr operon (EC
           3.5.-.-) (ORF 5)
          Length = 261

 Score = 80.5 bits (197), Expect = 5e-15
 Identities = 62/187 (33%), Positives = 103/187 (54%), Gaps = 24/187 (12%)

Query: 136 LFNTHVVVDDTGKIQTTYRKIHLFDVDVPGGRVYKESNFTESGKDI-----VAVDSPIGR 190
           +FNT   V+ +G++   Y K+HL    VP   + +E  F  +G+ +     ++  + + +
Sbjct: 91  IFNTAFSVNKSGQLINEYDKVHL----VP---MLREHEFLTAGEYVAEPFQLSDGTYVTQ 143

Query: 191 LGLSVCYDLRFPELYQLLRF--QHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVI 248
           L   +CYDLRFPEL   LR+  + GA+I    A +  ++   HW  LL+ARAIEN  +VI
Sbjct: 144 L---ICYDLRFPEL---LRYPARSGAKIAFYVAQWP-MSRLQHWHSLLKARAIENNMFVI 196

Query: 249 AAAQAGTHNDKRESYGDTLIIDPWGTVVGRLPDRLSTGIVVADIDLSLVDSVREKMPIAK 308
                G  +   E  G +++I+P G +VG L +  S  I+  D++L+ V+  RE +P+ K
Sbjct: 197 GTNSTG-FDGNTEYAGHSIVINPNGDLVGELNE--SADILTVDLNLNEVEQQRENIPVFK 253

Query: 309 VIVCNVY 315
            I  ++Y
Sbjct: 254 SIKLDLY 260


>NRL1_ARATH (P32961) Nitrilase 1 (EC 3.5.5.1)
          Length = 346

 Score = 80.5 bits (197), Expect = 5e-15
 Identities = 78/302 (25%), Positives = 137/302 (44%), Gaps = 56/302 (18%)

Query: 41  TNSVRVAAAQMTSI-TDLASNFSTCSRLVKEAASAGAKLLCFPEAFSFV---GAKDGDSV 96
           + +VRV   Q +++  D  +      + + EAAS GA+L+ FPE F      G + G +V
Sbjct: 22  STTVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAV 81

Query: 97  SI---------------AQPLDGPIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHV 141
            +               A  + GP + +   +AR++ ++L +G  +++G     L+ T +
Sbjct: 82  GVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYT---LYCTVL 138

Query: 142 VVDDTGKIQTTYRKIHLFDVDVPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLRF 201
                G+    +RK+    ++     ++ + +    G  I   D+PIG+LG ++C++ R 
Sbjct: 139 FFSPQGQFLGKHRKLMPTSLE---RCIWGQGD----GSTIPVYDTPIGKLGAAICWENRM 191

Query: 202 PELYQLLRFQHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQ--------- 252
           P LY+   +  G ++   P A     G   W+  +   AIE  C+V++A Q         
Sbjct: 192 P-LYRTALYAKGIELYCAPTA----DGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPD 246

Query: 253 ------AGTHNDKRE----SYGDTLIIDPWGTVVGRLPDRLSTGIVVADIDLSLVDSVRE 302
                    ++DK      S G ++II P G V+   P+  S G+V ADIDL   D  R 
Sbjct: 247 HPDYLFTDWYDDKEHDSIVSQGGSVIISPLGQVLAG-PNFESEGLVTADIDLG--DIARA 303

Query: 303 KM 304
           K+
Sbjct: 304 KL 305


>NRL2_ARATH (P32962) Nitrilase 2 (EC 3.5.5.1)
          Length = 339

 Score = 78.6 bits (192), Expect = 2e-14
 Identities = 78/316 (24%), Positives = 143/316 (44%), Gaps = 61/316 (19%)

Query: 32  VTTGEST-----MATNSVRVAAAQMTSI-TDLASNFSTCSRLVKEAASAGAKLLCFPEAF 85
           ++T E+T      ++  VR    Q +++  D  +     ++ + EAAS G++L+ FPEAF
Sbjct: 1   MSTSENTPFNGVASSTIVRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAF 60

Query: 86  SFV---GAKDGDSVSI---------------AQPLDGPIMDQYCSLARESSIWLSLGGFQ 127
                 G + G  V +               A  + GP +++   LA +++++L +G  +
Sbjct: 61  IGGYPRGFRFGLGVGVHNEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIE 120

Query: 128 EKGSDPRHLFNTHVVVDDTGKIQTTYRKIHLFDVDVPGGRVYKESNFTESGKDIVAVDSP 187
           + G     L+ T +     G+    +RK+    ++     ++ + +    G  I   D+P
Sbjct: 121 KDGYT---LYCTALFFSPQGQFLGKHRKLMPTSLE---RCIWGQGD----GSTIPVYDTP 170

Query: 188 IGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYV 247
           IG+LG ++C++ R P LY+   +  G ++   P A     G   W+  +   AIE  C+V
Sbjct: 171 IGKLGAAICWENRMP-LYRTALYAKGIELYCAPTA----DGSKEWQSSMLHIAIEGGCFV 225

Query: 248 IAAAQ---------------AGTHNDKRE----SYGDTLIIDPWGTVVGRLPDRLSTGIV 288
           ++A Q                  ++DK      S G ++II P G V+   P+  S G++
Sbjct: 226 LSACQFCLRKDFPDHPDYLFTDWYDDKEPDSIVSQGGSVIISPLGQVLAG-PNFESEGLI 284

Query: 289 VADIDLSLVDSVREKM 304
            AD+DL   D  R K+
Sbjct: 285 TADLDLG--DVARAKL 298


>NRL3_ARATH (P46010) Nitrilase 3 (EC 3.5.5.1)
          Length = 346

 Score = 77.8 bits (190), Expect = 3e-14
 Identities = 76/302 (25%), Positives = 136/302 (44%), Gaps = 56/302 (18%)

Query: 41  TNSVRVAAAQMTSI-TDLASNFSTCSRLVKEAASAGAKLLCFPEAFSF---VGAKDGDSV 96
           +++VRV   Q +++  D  +      + + EAAS GAKL+ FPEAF      G + G +V
Sbjct: 22  SSTVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAV 81

Query: 97  SI---------------AQPLDGPIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTHV 141
            +               A  + GP +++   LA ++++ L +G  ++ G     L+ T +
Sbjct: 82  GVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYT---LYCTAL 138

Query: 142 VVDDTGKIQTTYRKIHLFDVDVPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLRF 201
                G+    +RK+    ++     ++ + +    G  I   D+PIG++G ++C++ R 
Sbjct: 139 FFSPQGQFLGKHRKVMPTSLE---RCIWGQGD----GSTIPVYDTPIGKIGAAICWENRM 191

Query: 202 PELYQLLRFQHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQ--------- 252
           P LY+   +  G +I   P A   +     W+  +   A+E  C+V++A Q         
Sbjct: 192 P-LYRTALYAKGIEIYCAPTADYSL----EWQASMIHIAVEGGCFVLSAHQFCKRREFPE 246

Query: 253 ----------AGTHNDKRESYGDTLIIDPWGTVVGRLPDRLSTGIVVADIDLSLVDSVRE 302
                         +D   S G ++II P G V+   P+  S G+V AD+DL   D  R 
Sbjct: 247 HPDYLFNDIVDTKEHDPTVSGGGSVIISPLGKVLAG-PNYESEGLVTADLDLG--DIARA 303

Query: 303 KM 304
           K+
Sbjct: 304 KL 305


>NRL4_TOBAC (Q42965) Nitrilase 4 (EC 3.5.5.1)
          Length = 349

 Score = 73.6 bits (179), Expect = 7e-13
 Identities = 75/293 (25%), Positives = 128/293 (43%), Gaps = 54/293 (18%)

Query: 40  ATNSVRVAAAQMTSIT-DLASNFSTCSRLVKEAASAGAKLLCFPEAF-------SFVGAK 91
           +T +VR    Q ++I  D  +      RL+ EAAS GA+L+ FPEAF       S  G  
Sbjct: 25  STPTVRATVVQASTIFYDTPATLVKAERLLAEAASYGAQLVVFPEAFIGGYPRGSTFGVS 84

Query: 92  DGDSV-----------SIAQPLDGPIMDQYCSLARESSIWLSLGGFQEKGSDPRHLFNTH 140
            G+             + A  + GP +D+  ++A +  ++L +G  +  G     L+ T 
Sbjct: 85  IGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGKYKVYLVMGVIERDGYT---LYCTV 141

Query: 141 VVVDDTGKIQTTYRKIHLFDVDVPGGRVYKESNFTESGKDIVAVDSPIGRLGLSVCYDLR 200
           +  D  G     +RKI      +P         F + G  I   D+P+G++G ++C++ R
Sbjct: 142 LFFDSQGHFLGKHRKI------MPTALERIIWGFGD-GSTIPVYDTPLGKIGAAICWENR 194

Query: 201 FPELYQLLRFQHGAQILLVPAAFTKVTGEAHWEILLRARAIENQCYVIAAAQ-------- 252
            P L +   +  G +I   P A ++      W+  +   A+E  C+V++A Q        
Sbjct: 195 MP-LLRTAMYAKGIEIYCAPTADSRDV----WQASMTHIALEGGCFVLSANQFCRRKDYP 249

Query: 253 -------AGTHNDKRES----YGDTLIIDPWGTVVGRLPDRLSTGIVVADIDL 294
                  +GT  D         G ++II P G V+   P+ +   ++ AD+DL
Sbjct: 250 PPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAG-PNYVGEALISADLDL 301


>BUP1_HUMAN (Q9UBR1) Beta-ureidopropionase (EC 3.5.1.6)
           (Beta-alanine synthase) (N-carbamoyl-beta-alanine
           amidohydrolase) (BUP-1)
          Length = 384

 Score = 70.5 bits (171), Expect = 6e-12
 Identities = 63/256 (24%), Positives = 109/256 (41%), Gaps = 26/256 (10%)

Query: 67  LVKEAASAGAKLLCFPEA----FSFVGAKDGDSVSIAQPLDGPIMDQYCSLARESSIWLS 122
           +V+ AA  G  ++CF EA    F+F   +       A+  +     ++C    ++   + 
Sbjct: 103 IVEVAAMCGVNIICFQEAWTMPFAFCTREKLPWTEFAESAEDGPTTRFCQKLAKNHDMVV 162

Query: 123 LGGFQEKGSDPRH-LFNTHVVVDDTGKIQTTYRKIHLFDVDVPGGRVYKESNFTESGKDI 181
           +    E+ S+    L+NT VV+ ++G +    RK H+  V    G   + + + E     
Sbjct: 163 VSPILERDSEHGDVLWNTAVVISNSGAVLGKTRKNHIPRV----GDFNESTYYMEGNLGH 218

Query: 182 VAVDSPIGRLGLSVCYDLRFPELYQLLRFQHGAQILLVPAAFTKVTGEAHWEILLRARAI 241
               +  GR+ +++CY    P L  L+   +GA+I+  P+A      E+ W I  R  AI
Sbjct: 219 PVFQTQFGRIAVNICYGRHHP-LNWLMYSINGAEIIFNPSATIGALSESLWPIEARNAAI 277

Query: 242 ENQCYVIAAAQAGT---------------HNDKRESYGDTLIIDPWGTVVGRLPDRLSTG 286
            N C+  A  + GT               H D    YG + +  P  +    L  R   G
Sbjct: 278 ANHCFTCAINRVGTEHFPNEFTSGDGKKAHQDFGYFYGSSYVAAPDSSRTPGL-SRSRDG 336

Query: 287 IVVADIDLSLVDSVRE 302
           ++VA +DL+L   V +
Sbjct: 337 LLVAKLDLNLCQQVND 352


  Database: sprot
    Posted date:  Nov 25, 2004 10:54 AM
  Number of letters in database: 59,974,054
  Number of sequences in database:  164,201
  
Lambda     K      H
   0.320    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,286,451
Number of Sequences: 164201
Number of extensions: 1535457
Number of successful extensions: 3603
Number of sequences better than 10.0: 78
Number of HSP's better than 10.0 without gapping: 31
Number of HSP's successfully gapped in prelim test: 47
Number of HSP's that attempted gapping in prelim test: 3480
Number of HSP's gapped (non-prelim): 85
length of query: 327
length of database: 59,974,054
effective HSP length: 110
effective length of query: 217
effective length of database: 41,911,944
effective search space: 9094891848
effective search space used: 9094891848
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 66 (30.0 bits)