BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145329.2 + phase: 0 /pseudo
(246 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 152 9e-37
YHB0_YEAST (P38748) Hypothetical 67.5 kDa protein in PRPS4-STE20... 33 0.63
YF48_ARCFU (O28724) Hypothetical protein AF1548 31 2.4
3MGH_STAAW (P65416) Putative 3-methyladenine DNA glycosylase (EC... 31 3.1
3MGH_STAAN (P65415) Putative 3-methyladenine DNA glycosylase (EC... 31 3.1
3MGH_STAAM (P65414) Putative 3-methyladenine DNA glycosylase (EC... 31 3.1
RPC2_ARATH (P56764) DNA-directed RNA polymerase beta'' chain (EC... 30 4.1
RLUB_VIBCH (Q9KSS7) Ribosomal large subunit pseudouridine syntha... 30 5.4
MTNA_BACSU (O31662) Methylthioribose-1-phosphate isomerase (EC 5... 30 5.4
LIP5_CANAL (Q9P8W0) Lipase 5 precursor (EC 3.1.1.3) 30 5.4
EB30_CAEEL (P34441) Abnormal embryogenesis protein 30 30 5.4
ACF4_ORYLA (Q800I7) C-type natriuretic peptide-4 precursor 30 5.4
DPOA_HUMAN (P09884) DNA polymerase alpha catalytic subunit (EC 2... 30 7.0
CLPB_PARUW (Q6MD97) Chaperone clpB 30 7.0
Y504_BORBU (O51457) Hypothetical UPF0144 protein BB0504 29 9.2
RPC2_WHEAT (Q9XPS9) DNA-directed RNA polymerase beta'' chain (EC... 29 9.2
RPC2_SINAL (Q9THV5) DNA-directed RNA polymerase beta'' chain (EC... 29 9.2
RPC2_MAIZE (P16025) DNA-directed RNA polymerase beta'' chain (EC... 29 9.2
DPOA_MOUSE (P33609) DNA polymerase alpha catalytic subunit (EC 2... 29 9.2
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 152 bits (383), Expect = 9e-37
Identities = 89/236 (37%), Positives = 137/236 (57%), Gaps = 10/236 (4%)
Query: 3 GSKWDIEKFTGDNDFGLWKVKMEAVLIQQKCEKALKGEGLLPVTMSQVEKTDMVDKVRSA 62
G K+++ KF GDN F W+ +M +LIQQ K L + P TM + D+ ++ SA
Sbjct: 3 GVKYEVAKFNGDNGFSTWQRRMRDLLIQQGLHKVLDVDSKKPDTMKAEDWADLDERAASA 62
Query: 63 IVLCLGDKVLREVAKEPTAASIWAKLESLYMTKSLAHRQFLK*QLYLFRMVESKAIIEQL 122
I L L D V+ + E TA IW +LESLYM+K+L ++ +LK QLY M E + L
Sbjct: 63 IRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLSHL 122
Query: 123 MEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKEGTVILEEVQAAL-- 180
FN ++ L N+ V++E+EDKAILL ++P S+++ T+L+GK T+ L++V +AL
Sbjct: 123 NVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHGKT-TIELKDVTSALLL 181
Query: 181 --RTKKLTKSKDLRVHEHGEGLSVLR--GNDGGRGNRRKSGNKSRS---ECFNCHK 229
+ +K +++ + G G S R N G G R KS N+S+S C+NC++
Sbjct: 182 NEKMRKKPENQGQALITEGRGRSYQRSSNNYGRSGARGKSKNRSKSRVRNCYNCNQ 237
>YHB0_YEAST (P38748) Hypothetical 67.5 kDa protein in PRPS4-STE20
intergenic region
Length = 585
Score = 33.1 bits (74), Expect = 0.63
Identities = 29/121 (23%), Positives = 56/121 (45%), Gaps = 17/121 (14%)
Query: 83 SIWAKLESLYMTKSLAHRQFLK*QLYLFRMVESKAIIEQLMEFNKILDDLENIEVQLEDE 142
S+ +E L + + +++ K ++ +E ++ + ++ N LD L + QLE E
Sbjct: 462 SLKKSMEDLKLQFQVTQKEWQKREMAQKSKLEEDMLVIEGLQAN--LDHLSKKQEQLERE 519
Query: 143 DKAI-------------LLFCAIPKSFESFKDTMLYGKEGTVILEEVQAALRTKKLTKSK 189
+KA+ L+F S E FKD KEGT+++++ A + K K +
Sbjct: 520 NKALEESKQDLEEQVKDLMFYL--DSQEKFKDADESVKEGTILIQQPHGAAQASKSKKKR 577
Query: 190 D 190
+
Sbjct: 578 N 578
>YF48_ARCFU (O28724) Hypothetical protein AF1548
Length = 174
Score = 31.2 bits (69), Expect = 2.4
Identities = 15/47 (31%), Positives = 29/47 (60%), Gaps = 5/47 (10%)
Query: 34 EKALKGEGLLPVTMSQVEKTDMVDKVRSAIVLC-----LGDKVLREV 75
E L+ +GL P+T+ +++K + + VR+ +V C G++ LRE+
Sbjct: 109 EVLLESKGLYPITILRIDKEVLDELVRAGLVFCRDVVSAGEEKLREI 155
>3MGH_STAAW (P65416) Putative 3-methyladenine DNA glycosylase (EC
3.2.2.-)
Length = 202
Score = 30.8 bits (68), Expect = 3.1
Identities = 22/79 (27%), Positives = 36/79 (44%), Gaps = 6/79 (7%)
Query: 160 KDTMLYGKEGTVILEEVQAALRTKKLTKSKDL------RVHEHGEGLSVLRGNDGGRGNR 213
K T LY + GT+ + L +TKS+ + R E EGLS + N G +G
Sbjct: 57 KVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEGLSAMFRNRGKKGYE 116
Query: 214 RKSGNKSRSECFNCHKMVE 232
+G ++ FN + ++
Sbjct: 117 VTNGPGKWTKAFNIPRAID 135
>3MGH_STAAN (P65415) Putative 3-methyladenine DNA glycosylase (EC
3.2.2.-)
Length = 202
Score = 30.8 bits (68), Expect = 3.1
Identities = 22/79 (27%), Positives = 36/79 (44%), Gaps = 6/79 (7%)
Query: 160 KDTMLYGKEGTVILEEVQAALRTKKLTKSKDL------RVHEHGEGLSVLRGNDGGRGNR 213
K T LY + GT+ + L +TKS+ + R E EGLS + N G +G
Sbjct: 57 KVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEGLSAMFRNRGKKGYE 116
Query: 214 RKSGNKSRSECFNCHKMVE 232
+G ++ FN + ++
Sbjct: 117 VTNGPGKWTKAFNIPRAID 135
>3MGH_STAAM (P65414) Putative 3-methyladenine DNA glycosylase (EC
3.2.2.-)
Length = 202
Score = 30.8 bits (68), Expect = 3.1
Identities = 22/79 (27%), Positives = 36/79 (44%), Gaps = 6/79 (7%)
Query: 160 KDTMLYGKEGTVILEEVQAALRTKKLTKSKDL------RVHEHGEGLSVLRGNDGGRGNR 213
K T LY + GT+ + L +TKS+ + R E EGLS + N G +G
Sbjct: 57 KVTSLYKRGGTIYAHVMHTHLLINFVTKSEGIPEGVLIRAIEPEEGLSAMFRNRGKKGYE 116
Query: 214 RKSGNKSRSECFNCHKMVE 232
+G ++ FN + ++
Sbjct: 117 VTNGPGKWTKAFNIPRAID 135
>RPC2_ARATH (P56764) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1376
Score = 30.4 bits (67), Expect = 4.1
Identities = 18/76 (23%), Positives = 37/76 (48%), Gaps = 7/76 (9%)
Query: 119 IEQLMEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKEGTVILEEVQA 178
+EQ++E I N+E +++ +K I IP F L G E T++ +
Sbjct: 1144 VEQVLEVRSIDSISLNLEKRIKGWNKCITRILGIPWGF-------LIGAELTIVQSRISL 1196
Query: 179 ALRTKKLTKSKDLRVH 194
+ +K+ +S+ +++H
Sbjct: 1197 VNKIQKVYRSQGVQIH 1212
>RLUB_VIBCH (Q9KSS7) Ribosomal large subunit pseudouridine synthase
B (EC 4.2.1.70) (Pseudouridylate synthase) (Uracil
hydrolyase)
Length = 321
Score = 30.0 bits (66), Expect = 5.4
Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Query: 170 TVILEEVQAALRTKKLTKSKDLR--VHEHGEGLSVLRGNDGGRGNRRKSGNKS 220
TV+ AA R ++ T+S+ +R V H E +S G G RRK G +S
Sbjct: 251 TVLNMAPDAATRKRERTRSQKIRRAVRRHEERISATPGRSGKAPARRKPGGES 303
>MTNA_BACSU (O31662) Methylthioribose-1-phosphate isomerase (EC
5.3.1.23) (MTR-1-P isomerase)
Length = 353
Score = 30.0 bits (66), Expect = 5.4
Identities = 25/91 (27%), Positives = 42/91 (45%), Gaps = 3/91 (3%)
Query: 119 IEQLMEFNKILDDL--ENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKEGTVILEEV 176
+E + N+ +L E I++Q+EDE+ L+ + F+ M G++
Sbjct: 111 VENAISVNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLFKKGDRIMTICNAGSIATSRY 170
Query: 177 QAALRTKKLTKSKDLRVHEHG-EGLSVLRGN 206
AL L K KDL +H + E VL+G+
Sbjct: 171 GTALAPFYLAKQKDLGLHIYACETRPVLQGS 201
>LIP5_CANAL (Q9P8W0) Lipase 5 precursor (EC 3.1.1.3)
Length = 463
Score = 30.0 bits (66), Expect = 5.4
Identities = 21/64 (32%), Positives = 31/64 (47%), Gaps = 4/64 (6%)
Query: 131 DLENIEVQLEDEDKAILL---FCAIPKSFESFKDTMLYGKE-GTVILEEVQAALRTKKLT 186
DL Q E A LL + + +E K T GK+ G +L ++AAL++ K+T
Sbjct: 121 DLTTFVTQAEMYLMAPLLDQGYYVVSPDYEGPKSTFTIGKQSGQAVLNSIRAALKSGKIT 180
Query: 187 KSKD 190
KD
Sbjct: 181 NIKD 184
>EB30_CAEEL (P34441) Abnormal embryogenesis protein 30
Length = 1027
Score = 30.0 bits (66), Expect = 5.4
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 13/73 (17%)
Query: 108 YLFRM-----VESKAIIEQLMEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDT 162
YL+R+ VE K +IE E + D L+D+D LLF + + F D+
Sbjct: 563 YLYRLERIAEVERKKLIETFHEKFRFAD--------LDDDDLTPLLFQELDHPIDIFTDS 614
Query: 163 MLYGKEGTVILEE 175
M+ + + LEE
Sbjct: 615 MMEQNDSSPFLEE 627
>ACF4_ORYLA (Q800I7) C-type natriuretic peptide-4 precursor
Length = 121
Score = 30.0 bits (66), Expect = 5.4
Identities = 23/63 (36%), Positives = 31/63 (48%), Gaps = 11/63 (17%)
Query: 173 LEEVQAALRTKKLTKSKDLRVHEHGEGLSVLRGNDGGRGNRRKSGNK-----SRSECFNC 227
L++V++ +R + DLRV G+ ND R KSG+K SRS CF
Sbjct: 54 LDDVRSRMRLLR-----DLRVDTRARGMWARLLNDQPASRRHKSGSKKGGSTSRSGCFG- 107
Query: 228 HKM 230
HKM
Sbjct: 108 HKM 110
>DPOA_HUMAN (P09884) DNA polymerase alpha catalytic subunit (EC
2.7.7.7)
Length = 1462
Score = 29.6 bits (65), Expect = 7.0
Identities = 28/126 (22%), Positives = 54/126 (42%), Gaps = 5/126 (3%)
Query: 111 RMVESKAIIEQLMEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKE-- 168
++VE + ++QLM+ + DL +Q + KA+ L F + Y K
Sbjct: 917 KLVERRKQVKQLMKQQDLNPDLI---LQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLA 973
Query: 169 GTVILEEVQAALRTKKLTKSKDLRVHEHGEGLSVLRGNDGGRGNRRKSGNKSRSECFNCH 228
V + + + TK++ + +L V ++ N K GNK +SE +
Sbjct: 974 ALVTYKGREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLY 1033
Query: 229 KMVEVE 234
K++E++
Sbjct: 1034 KLLEID 1039
>CLPB_PARUW (Q6MD97) Chaperone clpB
Length = 868
Score = 29.6 bits (65), Expect = 7.0
Identities = 40/164 (24%), Positives = 77/164 (46%), Gaps = 18/164 (10%)
Query: 54 DMVDKVRSAIVLCLGDKVLREVAKEPTAASIWAKLESLYMTKS-LAHRQFLK*QLYLFRM 112
D++D+ S I + LG + L KE A + + E++ + LA + K + + ++
Sbjct: 392 DLIDESASLIRMQLGSRPLPIDNKERELAGLIVEQEAMKRESTPLAKNEVEKLEGRIAQI 451
Query: 113 VESKAII-EQLMEFNKILDDLENIEVQLED---EDKAI-----------LLFCAIPKSFE 157
E I+ EQ + KI++ L+ + +LE E++A L + IP+ +
Sbjct: 452 KEELKILREQWDQEKKIIESLKEKKDKLEKLRFEEEAAERKADYNRVAELRYNLIPQLQK 511
Query: 158 SFKD--TMLYGKEGTVILEEVQAALRTKKLTKSKDLRVHEHGEG 199
++ T L K ++ EEV +L + ++K + VH+ EG
Sbjct: 512 EIEEAQTQLNNKPNRLLQEEVDESLIAQIVSKWTGIPVHKMLEG 555
>Y504_BORBU (O51457) Hypothetical UPF0144 protein BB0504
Length = 510
Score = 29.3 bits (64), Expect = 9.2
Identities = 19/71 (26%), Positives = 36/71 (49%), Gaps = 1/71 (1%)
Query: 14 DNDFGLWKVKMEAVLIQQKCEKALKGEGLLPVTMSQVEKTDMVDKVRSAIVLCLGDKVLR 73
+N GL + ++I+ K E K + + + S+ E + DKV I++ +++
Sbjct: 133 ENISGLTREDARKIVIE-KVEHESKRDAQVIINKSEQEAQLLADKVAKDILVSTMQRIVT 191
Query: 74 EVAKEPTAASI 84
EV+ E T AS+
Sbjct: 192 EVSSEFTVASV 202
>RPC2_WHEAT (Q9XPS9) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1479
Score = 29.3 bits (64), Expect = 9.2
Identities = 18/76 (23%), Positives = 36/76 (46%), Gaps = 7/76 (9%)
Query: 119 IEQLMEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKEGTVILEEVQA 178
+EQ+ E I N+E ++ED ++ I +P F L G E T+ +
Sbjct: 1249 VEQIFEARSIDSLSPNLERRIEDWNERIPRILGVPWGF-------LIGAELTIAQSRISL 1301
Query: 179 ALRTKKLTKSKDLRVH 194
+ +K+ +S+ +++H
Sbjct: 1302 VNKIQKVYRSQGVQIH 1317
>RPC2_SINAL (Q9THV5) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1384
Score = 29.3 bits (64), Expect = 9.2
Identities = 17/76 (22%), Positives = 37/76 (48%), Gaps = 7/76 (9%)
Query: 119 IEQLMEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKEGTVILEEVQA 178
+EQ++E I N+E +++ ++ I IP F L G E T++ +
Sbjct: 1152 VEQVLEVRSIDSISLNLEKRIKGWNRCITRILGIPWGF-------LIGAELTIVQSRISL 1204
Query: 179 ALRTKKLTKSKDLRVH 194
+ K++ +S+ +++H
Sbjct: 1205 VNKIKQVYRSQGVQIH 1220
>RPC2_MAIZE (P16025) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1527
Score = 29.3 bits (64), Expect = 9.2
Identities = 18/76 (23%), Positives = 36/76 (46%), Gaps = 7/76 (9%)
Query: 119 IEQLMEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKEGTVILEEVQA 178
+EQ+ E I N+E ++ED ++ I +P F L G E T+ +
Sbjct: 1286 VEQIFEARSIDSLSPNLERRIEDWNERIPRILGVPWGF-------LIGAELTIAQSRISL 1338
Query: 179 ALRTKKLTKSKDLRVH 194
+ +K+ +S+ +++H
Sbjct: 1339 VNKIQKVYRSQGVQIH 1354
>DPOA_MOUSE (P33609) DNA polymerase alpha catalytic subunit (EC
2.7.7.7)
Length = 1465
Score = 29.3 bits (64), Expect = 9.2
Identities = 28/126 (22%), Positives = 53/126 (41%), Gaps = 5/126 (3%)
Query: 111 RMVESKAIIEQLMEFNKILDDLENIEVQLEDEDKAILLFCAIPKSFESFKDTMLYGKE-- 168
++VE + ++QLM+ + DL +Q + KA+ L F + Y K
Sbjct: 921 KLVERRKQVKQLMKQQDLNPDLV---LQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLA 977
Query: 169 GTVILEEVQAALRTKKLTKSKDLRVHEHGEGLSVLRGNDGGRGNRRKSGNKSRSECFNCH 228
V + + + TK + + +L V ++ N K GNK +SE +
Sbjct: 978 ALVTYKGREILMHTKDMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLY 1037
Query: 229 KMVEVE 234
K++E++
Sbjct: 1038 KLLEID 1043
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.323 0.138 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,540,169
Number of Sequences: 164201
Number of extensions: 1023216
Number of successful extensions: 3094
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 18
Number of HSP's that attempted gapping in prelim test: 3088
Number of HSP's gapped (non-prelim): 21
length of query: 246
length of database: 59,974,054
effective HSP length: 107
effective length of query: 139
effective length of database: 42,404,547
effective search space: 5894232033
effective search space used: 5894232033
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 64 (29.3 bits)
Medicago: description of AC145329.2