BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145222.7 - phase: 0
(435 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
G160_HUMAN (Q9UJ42) Probable G protein-coupled receptor 160 33 1.1
DNAK_PSEPK (Q88DU2) Chaperone protein dnaK (Heat shock protein 7... 33 1.1
GALC_MACMU (O02791) Galactocerebrosidase precursor (EC 3.2.1.46)... 33 1.4
DNAK_PSESM (Q87WP0) Chaperone protein dnaK (Heat shock protein 7... 33 1.4
IUCA_ECOLI (Q47316) Aerobactin siderophore biosynthesis protein ... 32 2.4
DNAK_CANBF (Q7VQL4) Chaperone protein dnaK (Heat shock protein 7... 32 4.2
VE2_HPV60 (Q80944) Regulatory protein E2 31 5.5
VATN_HUMAN (O75787) Vacuolar ATP synthase membrane sector associ... 31 5.5
DPOL_PYRKO (P77933) DNA polymerase (EC 2.7.7.7) [Contains: Endon... 31 5.5
DNAK_PSESG (Q9WWG9) Chaperone protein dnaK (Heat shock protein 7... 31 5.5
DAL5_YEAST (P15365) Allantoate permease 31 5.5
P2YE_MOUSE (Q9ESG6) P2Y purinoceptor 14 (P2Y14) (UDP-glucose rec... 31 7.1
PURQ_CORGL (Q8NMI4) Phosphoribosylformylglycinamidine synthase I... 30 9.3
GTT2_HUMAN (P30712) Glutathione S-transferase theta 2 (EC 2.5.1.... 30 9.3
CODB_ECOLI (P25525) Cytosine permease 30 9.3
>G160_HUMAN (Q9UJ42) Probable G protein-coupled receptor 160
Length = 338
Score = 33.5 bits (75), Expect = 1.1
Identities = 22/72 (30%), Positives = 37/72 (50%), Gaps = 4/72 (5%)
Query: 42 MLFSVAVGTLGVVLMTLGCLHEKHLEEFLEYFRFGLWWVALGVASSIGLGSGLHTFVL-- 99
+LF + +G + + ++TLG + + F+EYF L +V L + +I + FVL
Sbjct: 27 LLFLIILGKILLNILTLGMRRKNTCQNFMEYFCISLAFVDLLLLVNISIILYFRDFVLLS 86
Query: 100 --YLGPHIALFT 109
+ HI LFT
Sbjct: 87 IRFTKYHICLFT 98
>DNAK_PSEPK (Q88DU2) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 641
Score = 33.5 bits (75), Expect = 1.1
Identities = 16/57 (28%), Positives = 26/57 (45%), Gaps = 7/57 (12%)
Query: 103 PHIALFTIKAMQCGRV-------DLKSAPYDTIQLNRGPSWLDKNCSQFGPPLFQSE 152
PH LF +K + R D+K PY ++ N G +W++ + PP +E
Sbjct: 62 PHNTLFAVKRLIGRRFEEDVVQKDIKLVPYKIVKANNGDAWVEAAGKEMAPPQISAE 118
>GALC_MACMU (O02791) Galactocerebrosidase precursor (EC 3.2.1.46)
(GALCERase) (Galactosylceramidase) (Galactosylceramide
beta-galactosidase) (Galactocerebroside
beta-galactosidase)
Length = 669
Score = 33.1 bits (74), Expect = 1.4
Identities = 38/158 (24%), Positives = 60/158 (37%), Gaps = 27/158 (17%)
Query: 26 YIKKTMLYLLAKGGWLMLFSVAVGTLGVVLMTLGCLHEKHLEEFLEYFRFG--------- 76
Y KT+ +L KGG + + +G L +++ T+ H K + FL YF
Sbjct: 340 YYLKTVGHL-EKGGSYVALTDGLGNLTIIIETMSHKHSKCIRPFLPYFNVSQQFATFVLK 398
Query: 77 ----------LWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGRVDLKSAPYD 126
+W+ LG S L L + L+L FT+K + L
Sbjct: 399 GSFSEIPELQVWYTKLGKTSERFLFKQLDS--LWLLDSNGSFTLKLQEDELFTL-----T 451
Query: 127 TIQLNRGPSWLDKNCSQFGPPLFQSEYGTRVPLSSILP 164
T+ R S+L SQ P ++ ++ P S P
Sbjct: 452 TLTTGRKGSYLPPPKSQRFPSTYKDDFNVDYPFFSEAP 489
>DNAK_PSESM (Q87WP0) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 638
Score = 33.1 bits (74), Expect = 1.4
Identities = 14/57 (24%), Positives = 28/57 (48%), Gaps = 7/57 (12%)
Query: 103 PHIALFTIKAM-------QCGRVDLKSAPYDTIQLNRGPSWLDKNCSQFGPPLFQSE 152
PH L+ +K + Q + D++ PY ++ + G +W++ N + PP +E
Sbjct: 62 PHNTLYAVKRLIGRKFDEQVVQKDIQMVPYKIVKADNGDAWVEVNGQKMAPPQISAE 118
>IUCA_ECOLI (Q47316) Aerobactin siderophore biosynthesis protein
iucA
Length = 575
Score = 32.3 bits (72), Expect = 2.4
Identities = 30/104 (28%), Positives = 45/104 (42%), Gaps = 16/104 (15%)
Query: 135 SWLDKNCSQFGPPLF--QSEYGTRVPLSSILPQVQMEAVLWGLGTAIGELPPYFISRAAR 192
+W+D C Q PLF +++YG + VQM +G+LP FI R +
Sbjct: 399 AWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQM----------LGDLPVGFIYRDCQ 448
Query: 193 LSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFFTILVLAS 236
S A E LD+ D+ I F+ Q L +F +L +
Sbjct: 449 GSAFMPHATEWLDTIDEAQAENI----FTREQLLRYFPYYLLVN 488
>DNAK_CANBF (Q7VQL4) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 643
Score = 31.6 bits (70), Expect = 4.2
Identities = 19/57 (33%), Positives = 24/57 (41%), Gaps = 7/57 (12%)
Query: 103 PHIALFTIKAMQCGRV-------DLKSAPYDTIQLNRGPSWLDKNCSQFGPPLFQSE 152
P LF IK + R D+K PY I N G +WLD + PP +E
Sbjct: 62 PKNTLFAIKRLIGRRFQDQEVQRDVKIMPYKIISSNNGDAWLDIKGQKIAPPQISAE 118
>VE2_HPV60 (Q80944) Regulatory protein E2
Length = 404
Score = 31.2 bits (69), Expect = 5.5
Identities = 19/55 (34%), Positives = 27/55 (48%), Gaps = 3/55 (5%)
Query: 175 LGTAIGELPPYFISRAARLSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFF 229
+G+ LP ISR ARL G D L KG+ N +KCW + ++ +F
Sbjct: 298 VGSRHRSLPRSGISRLARLQGEARDPPILLI---KGLANSLKCWRYRLKKYTRYF 349
>VATN_HUMAN (O75787) Vacuolar ATP synthase membrane sector
associated protein M8-9 (V-ATPase M8.9 subunit)
Length = 100
Score = 31.2 bits (69), Expect = 5.5
Identities = 12/41 (29%), Positives = 24/41 (58%)
Query: 325 KAPHPVSPNTKGKKWDFSFTSIWNTVVWLMLMNFFIKIVNS 365
+A +P SP K++F ++ ++N V+W+M+ I+ S
Sbjct: 35 QAKNPASPYNLAYKYNFEYSVVFNMVLWIMIALALAVIITS 75
>DPOL_PYRKO (P77933) DNA polymerase (EC 2.7.7.7) [Contains:
Endonuclease PI-PkoI (EC 3.1.-.-) (Pko pol-1 intein)
(IVS-A); Endonuclease PI-PkoII (EC 3.1.-.-) (Pko pol-2
intein) (IVS-B)]
Length = 1671
Score = 31.2 bits (69), Expect = 5.5
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 3/76 (3%)
Query: 339 WDFSFTSIWNTVVWLMLMNFFIK---IVNSTAQTHLKKQQESEVAALTKKSDSDTQ*NAL 395
WD S +S N V W +L + + N + L ++++S K+ + N +
Sbjct: 342 WDVSRSSTGNLVEWFLLRKAYERNELAPNKPDEKELARRRQSYEGGYVKEPERGLWENIV 401
Query: 396 IIIFAVHPARSKLKFK 411
+ F HPA +K+ K
Sbjct: 402 YLDFRCHPADTKVVVK 417
>DNAK_PSESG (Q9WWG9) Chaperone protein dnaK (Heat shock protein 70)
(Heat shock 70 kDa protein) (HSP70)
Length = 638
Score = 31.2 bits (69), Expect = 5.5
Identities = 15/58 (25%), Positives = 28/58 (47%), Gaps = 9/58 (15%)
Query: 103 PHIALFTIKAMQCGRV--------DLKSAPYDTIQLNRGPSWLDKNCSQFGPPLFQSE 152
PH L+ +K + GR D++ PY ++ + G +W++ N + PP +E
Sbjct: 62 PHNTLYAVKRL-IGRKFEEDVVQKDIQMVPYKIVKADNGDAWVEVNGQKMAPPQISAE 118
>DAL5_YEAST (P15365) Allantoate permease
Length = 543
Score = 31.2 bits (69), Expect = 5.5
Identities = 19/84 (22%), Positives = 36/84 (42%), Gaps = 3/84 (3%)
Query: 240 PLFDLAGIMCGQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIENEFIWVL 299
PLF + + IPFW + L+ I A++ + ++ N + ++W +
Sbjct: 369 PLFGILAVYAANKKIPFWKYKLSWAIFAAVLA--LIASCMLGFATNSKKARLAGAYLWYI 426
Query: 300 SHIPGFASVLPKLTANLHAMKDKY 323
S + F VL ++AN K+
Sbjct: 427 SPV-SFICVLSNISANSSGYSKKW 449
>P2YE_MOUSE (Q9ESG6) P2Y purinoceptor 14 (P2Y14) (UDP-glucose
receptor) (G protein-coupled receptor 105)
Length = 338
Score = 30.8 bits (68), Expect = 7.1
Identities = 21/73 (28%), Positives = 39/73 (52%), Gaps = 9/73 (12%)
Query: 333 NTKGKKW----DFSFTSIWNTVVWLMLMNFFIKIVNSTAQTHLKKQQESEVAALTKKSDS 388
N G+KW ++ F SI+ VV+L+L+ F+ I ++HLK ++ S + K S
Sbjct: 177 NELGRKWHKASNYIFVSIF-WVVFLLLIVFYTAITRKIFKSHLKSRKNS----TSVKRKS 231
Query: 389 DTQ*NALIIIFAV 401
+++++F V
Sbjct: 232 SRNIFSIVLVFVV 244
>PURQ_CORGL (Q8NMI4) Phosphoribosylformylglycinamidine synthase I
(EC 6.3.5.3) (FGAM synthase I)
Length = 223
Score = 30.4 bits (67), Expect = 9.3
Identities = 22/73 (30%), Positives = 36/73 (49%)
Query: 174 GLGTAIGELPPYFISRAARLSGSRMDAMEELDSEDKGVLNQIKCWFFSHTQHLNFFTILV 233
G+ T G L +RAAR++G+ + ++ D + KGV + FS+ +L I
Sbjct: 6 GVITFPGTLDDVDAARAARIAGAEVISLWHADEDLKGVDAVVVPGGFSYGDYLRTGAISA 65
Query: 234 LASVPNPLFDLAG 246
LA V + + AG
Sbjct: 66 LAPVMQSVIEQAG 78
>GTT2_HUMAN (P30712) Glutathione S-transferase theta 2 (EC 2.5.1.18)
(GST class-theta 2)
Length = 243
Score = 30.4 bits (67), Expect = 9.3
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 3/65 (4%)
Query: 250 GQFGIPFWNFFLATLIGKAIIKTHIQTVFIISVCNNQLLDWIENEFIWVLSHIPGFASVL 309
G FGIP W L LIG + + ++ +Q L W+E++F+ + G L
Sbjct: 107 GTFGIPLWVQVLGPLIGVQVPEEKVER---NRTAMDQALQWLEDKFLGDRPFLAGQQVTL 163
Query: 310 PKLTA 314
L A
Sbjct: 164 ADLMA 168
>CODB_ECOLI (P25525) Cytosine permease
Length = 419
Score = 30.4 bits (67), Expect = 9.3
Identities = 22/77 (28%), Positives = 38/77 (48%), Gaps = 4/77 (5%)
Query: 20 TLAVIQYIKKTMLYLLAKG---GWLMLFSVAVGTLGVVLMTLGCLHEKHLEEFLEYFRFG 76
TL+VI I T+ L GWL S A+ +G V++ ++ + E F
Sbjct: 301 TLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPPVGGVIIADYLMNRRRYEHFATTRMMS 360
Query: 77 LWWVALGVASSIGLGSG 93
+ WVA+ +A ++G+ +G
Sbjct: 361 VNWVAI-LAVALGIAAG 376
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.331 0.143 0.461
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,669,771
Number of Sequences: 164201
Number of extensions: 2051234
Number of successful extensions: 5630
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 5625
Number of HSP's gapped (non-prelim): 17
length of query: 435
length of database: 59,974,054
effective HSP length: 113
effective length of query: 322
effective length of database: 41,419,341
effective search space: 13337027802
effective search space used: 13337027802
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 67 (30.4 bits)
Medicago: description of AC145222.7