BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145220.5 + phase: 0 /pseudo
(141 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NLFA_DROME (Q86NP2) Negative elongation factor A homolog 34 0.089
GLG1_MOUSE (Q61543) Golgi apparatus protein 1 precursor (Golgi s... 34 0.089
BRM_DROME (P25439) Homeotic gene regulator (Brahma protein) 34 0.12
GDA9_WHEAT (P18573) Alpha/beta-gliadin MM1 precursor (Prolamin) 33 0.15
SSN6_YEAST (P14922) Glucose repression mediator protein 33 0.20
SUZ2_DROME (P25172) Suppressor 2 of zeste protein (Protein poste... 33 0.26
KCN3_RAT (P70605) Small conductance calcium-activated potassium ... 33 0.26
KCN3_HUMAN (Q9UGI6) Small conductance calcium-activated potassiu... 32 0.44
HUNB_DROLI (O46244) Hunchback protein (Fragments) 32 0.44
HCN1_RAT (Q9JKB0) Potassium/sodium hyperpolarization-activated c... 32 0.44
GDA1_WHEAT (P04721) Alpha/beta-gliadin A-I precursor (Prolamin) 32 0.44
FTSK_ECOL6 (Q8FJC7) DNA translocase ftsK 32 0.58
GDA0_WHEAT (P02863) Alpha/beta-gliadin precursor (Prolamin) 31 0.75
GDA6_WHEAT (P04726) Alpha/beta-gliadin clone PW1215 precursor (P... 31 0.98
FXJ2_HUMAN (Q9P0K8) Forkhead box protein J2 (Fork head homologou... 31 0.98
CRF1_YARLI (P45815) Copper resistance protein CRF1 31 0.98
AMEY_HUMAN (Q99218) Amelogenin, Y isoform precursor 31 0.98
TOB1_RAT (Q8R5K6) Tob1 protein (Transducer of erbB-2 1) 30 1.3
TOB1_MOUSE (Q61471) Tob1 protein (Transducer of erbB-2 1) 30 1.3
MAK_HUMAN (P20794) Serine/threonine-protein kinase MAK (EC 2.7.1... 30 1.3
>NLFA_DROME (Q86NP2) Negative elongation factor A homolog
Length = 1248
Score = 34.3 bits (77), Expect = 0.089
Identities = 22/66 (33%), Positives = 32/66 (48%), Gaps = 1/66 (1%)
Query: 2 LPSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFP-LIPMLYAKLLPTLLLKGQAST*S 60
LP Q ++ QQQP Q+QQQQ +P P L +++ +P QA+T S
Sbjct: 714 LPQQPQQQQTQYILATPQQQPQQIQQQQQQQQPILPTLTSFSHSRPMPQTTTLYQATTPS 773
Query: 61 IASQVP 66
+ Q P
Sbjct: 774 GSGQTP 779
>GLG1_MOUSE (Q61543) Golgi apparatus protein 1 precursor (Golgi
sialoglycoprotein MG-160) (E-selectin ligand 1) (ESL-1)
(Selel)
Length = 1175
Score = 34.3 bits (77), Expect = 0.089
Identities = 17/34 (50%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 3 PSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTF 36
P+GQ Q P + + QQQPP QQ+QQQQ + F
Sbjct: 53 PAGQ-QPPQQPQLSQQQQQPPPQQQQQQQQQSLF 85
>BRM_DROME (P25439) Homeotic gene regulator (Brahma protein)
Length = 1638
Score = 33.9 bits (76), Expect = 0.12
Identities = 19/37 (51%), Positives = 21/37 (56%), Gaps = 2/37 (5%)
Query: 1 PLPSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFP 37
PLP PQV +Q QQQPP QQ+QQ Q P P
Sbjct: 290 PLPPQPPQVGGPPPIQ--QQQPPQQQQQQSQPPPPEP 324
>GDA9_WHEAT (P18573) Alpha/beta-gliadin MM1 precursor (Prolamin)
Length = 307
Score = 33.5 bits (75), Expect = 0.15
Identities = 19/50 (38%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Query: 1 PLPSGQPQV--PVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLP 48
P P QPQ P + Q+ QQQ QQ++QQQ + L +L +L+P
Sbjct: 113 PYPQSQPQYSQPQQPISQQQQQQQQQQQQKQQQQQQQQILQQILQQQLIP 162
>SSN6_YEAST (P14922) Glucose repression mediator protein
Length = 966
Score = 33.1 bits (74), Expect = 0.20
Identities = 19/56 (33%), Positives = 26/56 (45%)
Query: 3 PSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPTLLLKGQAST 58
P G+ + Q+ QQQ QQ+QQQ A P PL P+ + L + A T
Sbjct: 3 PGGEQTIMEQPAQQQQQQQQQQQQQQQQAAVPQQPLDPLTQSTAETWLSIASLAET 58
>SUZ2_DROME (P25172) Suppressor 2 of zeste protein (Protein
posterior sex combs)
Length = 1365
Score = 32.7 bits (73), Expect = 0.26
Identities = 19/50 (38%), Positives = 28/50 (56%), Gaps = 3/50 (6%)
Query: 1 PLPSGQPQVPVNAVVQRMQQQPPAQQRQQQQ-ARPTFPLIPMLYAKLLPT 49
P P+ +P+ Q+ QQQP QQ+QQQQ + F ++P + LPT
Sbjct: 595 PTPTAEPEQQQQQ--QQQQQQPQQQQQQQQQQQQQQFVVLPKIKDLTLPT 642
>KCN3_RAT (P70605) Small conductance calcium-activated potassium
channel protein 3 (SK3)
Length = 732
Score = 32.7 bits (73), Expect = 0.26
Identities = 20/38 (52%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Query: 1 PLPSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFPL 38
P P QPQ P Q+ QQQ QQ+QQQQA P PL
Sbjct: 50 PGPLLQPQPPQLQQQQQQQQQQQQQQQQQQQA-PLHPL 86
Score = 30.8 bits (68), Expect = 0.98
Identities = 19/50 (38%), Positives = 23/50 (46%), Gaps = 9/50 (18%)
Query: 1 PLPSGQPQVPVNAVVQRMQQQPPA---------QQRQQQQARPTFPLIPM 41
P PS P VP +Q QPP QQ+QQQQ + PL P+
Sbjct: 37 PPPSAPPAVPQQPPGPLLQPQPPQLQQQQQQQQQQQQQQQQQQQAPLHPL 86
>KCN3_HUMAN (Q9UGI6) Small conductance calcium-activated potassium
channel protein 3 (SK3) (SKCa3)
Length = 736
Score = 32.0 bits (71), Expect = 0.44
Identities = 19/36 (52%), Positives = 21/36 (57%), Gaps = 2/36 (5%)
Query: 3 PSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFPL 38
PS QPQ P Q+ QQQ QQ+QQQQ P PL
Sbjct: 57 PSLQPQPPQLQQQQQQQQQQQQQQQQQQQ--PPHPL 90
Score = 28.9 bits (63), Expect = 3.7
Identities = 13/31 (41%), Positives = 16/31 (50%)
Query: 1 PLPSGQPQVPVNAVVQRMQQQPPAQQRQQQQ 31
P P P P + +Q QPP Q+QQQQ
Sbjct: 42 PPPPAPPAAPQQPLGPSLQPQPPQLQQQQQQ 72
>HUNB_DROLI (O46244) Hunchback protein (Fragments)
Length = 198
Score = 32.0 bits (71), Expect = 0.44
Identities = 16/49 (32%), Positives = 26/49 (52%)
Query: 1 PLPSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPT 49
PLPS P N +++ +Q QQ+QQQQ + P+ + A + P+
Sbjct: 48 PLPSPNPPSNTNLQLEQYLKQQQQQQQQQQQQQQQQPMDTLCAAAMTPS 96
>HCN1_RAT (Q9JKB0) Potassium/sodium hyperpolarization-activated
cyclic nucleotide-gated channel 1
Length = 910
Score = 32.0 bits (71), Expect = 0.44
Identities = 17/35 (48%), Positives = 20/35 (56%), Gaps = 2/35 (5%)
Query: 3 PSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFP 37
P QPQ P Q+ QQQ QQ+QQQQ +P P
Sbjct: 747 PQPQPQQPQQQ--QQQQQQQQQQQQQQQQQQPQTP 779
>GDA1_WHEAT (P04721) Alpha/beta-gliadin A-I precursor (Prolamin)
Length = 262
Score = 32.0 bits (71), Expect = 0.44
Identities = 20/52 (38%), Positives = 27/52 (51%), Gaps = 4/52 (7%)
Query: 1 PLPSGQPQV--PVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPTL 50
P P QPQ P + Q+ QQQ QQ+QQQQ + I L +L+P +
Sbjct: 99 PYPQPQPQYSQPQQPISQQQQQQQQQQQQQQQQQQQIIQQI--LQQQLIPCM 148
>FTSK_ECOL6 (Q8FJC7) DNA translocase ftsK
Length = 1347
Score = 31.6 bits (70), Expect = 0.58
Identities = 19/48 (39%), Positives = 25/48 (51%), Gaps = 1/48 (2%)
Query: 6 QPQVPVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPTLLLK 53
QPQ PV Q Q Q P Q+ Q Q +P P++P LL LL++
Sbjct: 798 QPQQPVAPQQQYQQPQQPVTQQPQYQ-QPQQPVVPQPQDTLLHPLLMR 844
>GDA0_WHEAT (P02863) Alpha/beta-gliadin precursor (Prolamin)
Length = 286
Score = 31.2 bits (69), Expect = 0.75
Identities = 20/52 (38%), Positives = 28/52 (53%), Gaps = 5/52 (9%)
Query: 1 PLPSGQPQV--PVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPTL 50
P P QPQ P + Q+ QQQ QQ+QQQQ + L +L +L+P +
Sbjct: 99 PYPQPQPQYSQPQQPISQQQQQQQQQQQQQQQQQQ---ILQQILQQQLIPCM 147
>GDA6_WHEAT (P04726) Alpha/beta-gliadin clone PW1215 precursor
(Prolamin)
Length = 296
Score = 30.8 bits (68), Expect = 0.98
Identities = 20/51 (39%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Query: 1 PLPSGQPQVP---VNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLP 48
P P QPQ P Q+ QQQ QQ+QQQQ + L +L +L+P
Sbjct: 104 PYPQPQPQYPQPQQPISQQQAQQQQQQQQQQQQQQQQQQILQQILQQQLIP 154
>FXJ2_HUMAN (Q9P0K8) Forkhead box protein J2 (Fork head homologous
X)
Length = 574
Score = 30.8 bits (68), Expect = 0.98
Identities = 14/28 (50%), Positives = 16/28 (57%)
Query: 10 PVNAVVQRMQQQPPAQQRQQQQARPTFP 37
P N QQQPP Q+QQQQ +P P
Sbjct: 283 PSNNYYMYQQQQPPPPQQQQQQQQPPQP 310
Score = 28.9 bits (63), Expect = 3.7
Identities = 13/26 (50%), Positives = 16/26 (61%)
Query: 12 NAVVQRMQQQPPAQQRQQQQARPTFP 37
N + + QQ PP QQ+QQQQ P P
Sbjct: 286 NYYMYQQQQPPPPQQQQQQQQPPQPP 311
>CRF1_YARLI (P45815) Copper resistance protein CRF1
Length = 411
Score = 30.8 bits (68), Expect = 0.98
Identities = 17/48 (35%), Positives = 26/48 (53%)
Query: 16 QRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPTLLLKGQAST*SIAS 63
Q+ QQQ +QQ QQQQ +P P+L +PT + +T +A+
Sbjct: 119 QQAQQQSQSQQAQQQQQQPQPQASPILQQPQMPTPVHTTNVTTPPVAT 166
>AMEY_HUMAN (Q99218) Amelogenin, Y isoform precursor
Length = 192
Score = 30.8 bits (68), Expect = 0.98
Identities = 22/54 (40%), Positives = 26/54 (47%), Gaps = 5/54 (9%)
Query: 1 PLPSGQPQVPVNAVVQR---MQQQPPAQQRQQQQARPTFPLIPMLYAKLLPTLL 51
PLP+ QP P Q MQ QPP Q Q +P PL PM + LP +L
Sbjct: 121 PLPAQQPFQPQPVQPQPHQPMQPQPPVQPMQPLLPQP--PLPPMFPLRPLPPIL 172
>TOB1_RAT (Q8R5K6) Tob1 protein (Transducer of erbB-2 1)
Length = 365
Score = 30.4 bits (67), Expect = 1.3
Identities = 21/59 (35%), Positives = 28/59 (46%), Gaps = 5/59 (8%)
Query: 3 PSGQPQVPVNAVVQRMQQQPPAQQRQQQQARP---TFPLIPMLYAKLLPTLLLKGQAST 58
P QP P Q+ Q Q QQ+QQQQ +P T L P + P ++GQ S+
Sbjct: 249 PPSQPPPPPPPPQQQQQHQQQQQQQQQQQQQPQQQTSALSPNAKEFIFPN--MQGQGSS 305
>TOB1_MOUSE (Q61471) Tob1 protein (Transducer of erbB-2 1)
Length = 363
Score = 30.4 bits (67), Expect = 1.3
Identities = 22/55 (40%), Positives = 30/55 (54%), Gaps = 7/55 (12%)
Query: 4 SGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPTLLLKGQAST 58
S QP P+ Q+ QQQP QQ+QQQQ + T L P + P ++GQ S+
Sbjct: 256 SSQPPPPLP---QQQQQQP--QQQQQQQQQQTSALSPNAKEFIFPN--MQGQGSS 303
>MAK_HUMAN (P20794) Serine/threonine-protein kinase MAK (EC
2.7.1.37) (Male germ cell-associated kinase)
Length = 623
Score = 30.4 bits (67), Expect = 1.3
Identities = 18/49 (36%), Positives = 23/49 (46%), Gaps = 4/49 (8%)
Query: 1 PLPSGQPQVPVNAVVQRMQQQPPAQQRQQQQARPTFPLIPMLYAKLLPT 49
P S QP P+ QQPP QQ Q++ + FP I K +PT
Sbjct: 337 PKTSQQPLQPIQPPQNLSVQQPPKQQSQEKPPQTLFPSI----VKNMPT 381
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.352 0.154 0.540
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,243,982
Number of Sequences: 164201
Number of extensions: 459445
Number of successful extensions: 8101
Number of sequences better than 10.0: 112
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 44
Number of HSP's that attempted gapping in prelim test: 6508
Number of HSP's gapped (non-prelim): 795
length of query: 141
length of database: 59,974,054
effective HSP length: 99
effective length of query: 42
effective length of database: 43,718,155
effective search space: 1836162510
effective search space used: 1836162510
T: 11
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (22.0 bits)
S2: 60 (27.7 bits)
Medicago: description of AC145220.5