BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC145219.5 + phase: 0
(130 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
T257_ECOLI (P25239) Type IIS restriction enzyme Eco57I (EC 3.1.2... 37 0.008
C1AE_BACTL (Q03748) Pesticidial crystal protein cry1Ae (Insectic... 33 0.11
NIN_MOUSE (Q61043) Ninein 33 0.14
YC84_MYCPN (P75493) Hypothetical lipoprotein MPN284 precursor (A... 32 0.32
C1HB_BACTM (Q45718) Pesticidial crystal protein cry1Hb (Insectic... 32 0.32
C1DB_BACTU (Q45747) Pesticidial crystal protein cry1Db (Insectic... 32 0.32
COG8_YEAST (Q04632) Conserved oligomeric Golgi complex component 8 31 0.42
COAE_STAEP (Q8CS70) Dephospho-CoA kinase (EC 2.7.1.24) (Dephosph... 31 0.55
PIB3_HUMAN (Q01970) 1-phosphatidylinositol-4,5-bisphosphate phos... 30 0.71
MYBH_DICDI (P34127) Myb-like protein (Fragment) 30 0.71
YHP4_SCHPO (Q9USP9) Probable RNA-binding protein C902.04 30 0.93
YA27_SCHPO (Q09698) Hypothetical protein C2F7.07c in chromosome I 30 0.93
ARP_PLAFA (P04931) Asparagine-rich protein (AG319) (ARP) (Fragment) 30 0.93
S155_YEAST (P43612) SIT4-associating protein SAP155 30 1.2
RS20_WIGBR (Q8D2Q8) 30S ribosomal protein S20 30 1.2
MSS4_YEAST (P38994) Probable phosphatidylinositol-4-phosphate 5-... 30 1.2
C1CB_BACTG (P56953) Pesticidial crystal protein cry1Cb (Insectic... 30 1.2
MC70_YEAST (Q12411) Sporulation-specific protein 21 (Meiotic pla... 29 1.6
COAE_STAAW (P63832) Dephospho-CoA kinase (EC 2.7.1.24) (Dephosph... 29 1.6
COAE_STAAN (P63831) Dephospho-CoA kinase (EC 2.7.1.24) (Dephosph... 29 1.6
>T257_ECOLI (P25239) Type IIS restriction enzyme Eco57I (EC
3.1.21.4) (Endonuclease Eco57I) [Includes:
Adenine-specific methyltransferase activity Eco57IA (EC
2.1.1.72) (M.Eco57IA)]
Length = 998
Score = 37.0 bits (84), Expect = 0.008
Identities = 24/78 (30%), Positives = 41/78 (51%), Gaps = 3/78 (3%)
Query: 35 DYSDVEDAEDGDP--EAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGL 92
D+ D + E D Q LNK + Q Q DR++ I ++ E ++ +M D ++KN+
Sbjct: 922 DFDDPKQKEIHDTISSKQQYLNKLYSQTQKSADRDKIIFERQFEQEKIQM-DYLIKNLFD 980
Query: 93 IQELNGNISKVASLYSDL 110
+ +L+ I V LY +L
Sbjct: 981 LGDLDSEIPTVEDLYKNL 998
>C1AE_BACTL (Q03748) Pesticidial crystal protein cry1Ae
(Insecticidal delta-endotoxin CryIA(e)) (Crystaline
entomocidal protoxin) (134 kDa crystal protein)
Length = 1181
Score = 33.1 bits (74), Expect = 0.11
Identities = 32/118 (27%), Positives = 53/118 (44%), Gaps = 18/118 (15%)
Query: 10 AMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRA 69
A+ KK + +R TN Y + +++ D +N + Q+Q+ D N A
Sbjct: 875 ALARVKRAEKKWRDKREKLQLETNIVYKEAKESVDA-----LFVNSQYDQLQA--DTNIA 927
Query: 70 II----QQVNENQQSRMPDNMV---KNVGLIQELNGNISKVASLYSD----LNSDFTN 116
+I ++V+ Q++ +P+ V N G+ +EL G I SLY N DF N
Sbjct: 928 MIHTADKRVHRIQEAYLPELSVIPGVNAGIFEELEGRIFTAYSLYDARNVIKNGDFNN 985
>NIN_MOUSE (Q61043) Ninein
Length = 2035
Score = 32.7 bits (73), Expect = 0.14
Identities = 27/114 (23%), Positives = 54/114 (46%), Gaps = 5/114 (4%)
Query: 11 MEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAI 70
+ T S + Q + ++ + + +S++E++ED E+W L Q++ L RA
Sbjct: 1143 LASTGTSSVQRQECKTEASEASLDCFSELENSEDTRTESWD-LKSQISQLREQLTVLRAD 1201
Query: 71 IQQVNENQQSRMPDNMV--KNVGLIQELNGNISKVASLYSDLNSDFTNICHQQQ 122
+ +E +Q + D V K + +++ L S+ LY D + N C Q++
Sbjct: 1202 CDRASERKQDLLFDISVLKKKLKMLERLPEASSRYKVLYEDAARE--NSCLQEE 1253
>YC84_MYCPN (P75493) Hypothetical lipoprotein MPN284 precursor
(A65_orf794)
Length = 794
Score = 31.6 bits (70), Expect = 0.32
Identities = 27/115 (23%), Positives = 48/115 (41%), Gaps = 12/115 (10%)
Query: 18 SKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLN------KSFRQVQSVLDRNRAII 71
+ K+ RR +T T+ND D +++ E+W K F S+ D ++
Sbjct: 229 NSKSNRRSTDQSTQTHNDQGDASESDKKIKESWGDYEEVDGGLKGFTFKASIFDNWHDLL 288
Query: 72 QQVNENQQS--RMPDNMVKNVGLIQELNGNISKVASLYSDL----NSDFTNICHQ 120
+S ++ DN K IQ + G S SL++ + N D+ N ++
Sbjct: 289 DFSTRAAKSFKKIKDNNTKKGTDIQGILGVDSSANSLFTSVFAAGNGDYDNFFYK 343
>C1HB_BACTM (Q45718) Pesticidial crystal protein cry1Hb
(Insecticidal delta-endotoxin CryIH(b)) (Crystaline
entomocidal protoxin) (131 kDa crystal protein)
Length = 1155
Score = 31.6 bits (70), Expect = 0.32
Identities = 31/118 (26%), Positives = 53/118 (44%), Gaps = 18/118 (15%)
Query: 10 AMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRA 69
A+ KK + +R TN Y + +++ D N + ++Q+ D N A
Sbjct: 863 ALARVKRAEKKWRDKRETLQLETNIVYKEAKESVDA-----LFANSQYNRLQA--DTNIA 915
Query: 70 II----QQVNENQQSRMPDNMV---KNVGLIQELNGNISKVASLYSD----LNSDFTN 116
+I ++V+ +++ +P+ V N G+ +EL G I SLY NSDF N
Sbjct: 916 MIHAADKRVHRIREAYLPELSVIPGVNAGIFEELEGRIFTAFSLYDARNVIKNSDFNN 973
>C1DB_BACTU (Q45747) Pesticidial crystal protein cry1Db
(Insecticidal delta-endotoxin CryID(b)) (Crystaline
entomocidal protoxin) (131 kDa crystal protein)
Length = 1160
Score = 31.6 bits (70), Expect = 0.32
Identities = 31/118 (26%), Positives = 53/118 (44%), Gaps = 18/118 (15%)
Query: 10 AMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRA 69
A+ KK + +R TN Y + +++ D +N + Q+Q+ D N A
Sbjct: 859 ALARVKRAEKKWRDKREKLELETNIVYKEAKESVDA-----LFVNSQYDQLQA--DTNIA 911
Query: 70 II----QQVNENQQSRMPDNMV---KNVGLIQELNGNISKVASLYSD----LNSDFTN 116
+I ++V+ +++ +P+ V N G+ +EL G I SLY N DF N
Sbjct: 912 MIHAADKRVHSIREAYLPELSVIPGVNAGIFEELEGRIFTAYSLYDARNVIKNGDFNN 969
>COG8_YEAST (Q04632) Conserved oligomeric Golgi complex component 8
Length = 607
Score = 31.2 bits (69), Expect = 0.42
Identities = 24/84 (28%), Positives = 42/84 (49%), Gaps = 6/84 (7%)
Query: 37 SDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQEL 96
S ED + D E L++ R+ ++LD II + EN D++ K++ + EL
Sbjct: 48 SITEDIAEIDAEL-SALDRKIRK--TLLDNTSQIIGNILENDDRAQLDDIAKSLEQLWEL 104
Query: 97 NGNISKVAS---LYSDLNSDFTNI 117
+ NI+K A D+N++ +I
Sbjct: 105 DTNINKAADRNVTNDDINNESVSI 128
>COAE_STAEP (Q8CS70) Dephospho-CoA kinase (EC 2.7.1.24)
(Dephosphocoenzyme A kinase)
Length = 203
Score = 30.8 bits (68), Expect = 0.55
Identities = 16/71 (22%), Positives = 36/71 (50%), Gaps = 8/71 (11%)
Query: 41 DAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQ--------QVNENQQSRMPDNMVKNVGL 92
+ +D E W Q+ +++RN ++ Q++ +++SRM ++++ N+G
Sbjct: 121 ELQDTVDEVWVVYTSESIQIDRLMERNNLSLEDAKARVYSQISIDKKSRMANHVIDNLGD 180
Query: 93 IQELNGNISKV 103
EL N+ K+
Sbjct: 181 KLELKQNLQKL 191
>PIB3_HUMAN (Q01970) 1-phosphatidylinositol-4,5-bisphosphate
phosphodiesterase beta 3 (EC 3.1.4.11) (Phosphoinositide
phospholipase C) (PLC-beta-3) (Phospholipase C-beta-3)
Length = 1234
Score = 30.4 bits (67), Expect = 0.71
Identities = 22/59 (37%), Positives = 31/59 (52%), Gaps = 6/59 (10%)
Query: 24 RRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVNENQQSRM 82
R RP A +DVED ++G+ EA + RQVQS+L+ A QV+ Q R+
Sbjct: 1006 RLRPGAL---GGAADVEDTKEGEDEAKRYQEFQNRQVQSLLELREA---QVDAEAQRRL 1058
>MYBH_DICDI (P34127) Myb-like protein (Fragment)
Length = 451
Score = 30.4 bits (67), Expect = 0.71
Identities = 28/125 (22%), Positives = 51/125 (40%), Gaps = 10/125 (8%)
Query: 8 SSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKS--FRQVQSVLD 65
SS D + +N + NN+ + ED +D D N S F ++ ++
Sbjct: 35 SSNNSDNENDDNQNNNNNNNNNNNNNNEEEEEEDDDDDDDSQQNRHNISIPFTINKNSIN 94
Query: 66 RNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVASLYSDLNSDFTNICHQQQRSS 125
N +I +N N + M +NM N N N + ++ ++ NS+ +N +
Sbjct: 95 NNNNLINNIN-NNINNMNNNMNNN-------NNNNNMNNNINNNGNSNISNNNTPKVEKK 146
Query: 126 HNRGK 130
+GK
Sbjct: 147 KTKGK 151
>YHP4_SCHPO (Q9USP9) Probable RNA-binding protein C902.04
Length = 589
Score = 30.0 bits (66), Expect = 0.93
Identities = 25/102 (24%), Positives = 50/102 (48%), Gaps = 17/102 (16%)
Query: 5 YSPSSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDP-------EAWQTLNKSF 57
Y+PS ++R KK + +PT +D S+VE +ED DP E + + +
Sbjct: 312 YNPSKGF---HNRPKKFASHK----SPTTSDSSNVESSEDVDPASLLQNEEFHKLIEERQ 364
Query: 58 RQVQSVLDR---NRAIIQQVNENQQSRMPDNMVKNVGLIQEL 96
RQ + L R N+ ++++N+ ++ + + L+Q++
Sbjct: 365 RQHEERLKRINANKKALEELNQKKRELAQQQLKEQELLMQKI 406
>YA27_SCHPO (Q09698) Hypothetical protein C2F7.07c in chromosome I
Length = 607
Score = 30.0 bits (66), Expect = 0.93
Identities = 26/105 (24%), Positives = 43/105 (40%), Gaps = 11/105 (10%)
Query: 5 YSPSSAMEDTN----HRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQV 60
YSPS + N + + PS T D +DG AW TL+ FR +
Sbjct: 81 YSPSVKRKHVNGEGAEKGDHDTSNNGPSITKLRRKVRRTYDTKDGFV-AWNTLDDDFRPI 139
Query: 61 QSVLDRNRAIIQQVNENQQSRMPDNMVKNVGLIQELNGNISKVAS 105
+R+R I Q N +N++K ++ ++S ++S
Sbjct: 140 VPDQERSRKINPQKGNN------NNLLKENKSLKTTAKDLSDISS 178
>ARP_PLAFA (P04931) Asparagine-rich protein (AG319) (ARP) (Fragment)
Length = 537
Score = 30.0 bits (66), Expect = 0.93
Identities = 13/64 (20%), Positives = 31/64 (48%), Gaps = 1/64 (1%)
Query: 12 EDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNK-SFRQVQSVLDRNRAI 70
++ N+ N+ + NN+Y++ + ED D + W L + + + S++ + I
Sbjct: 440 KNNNNNINMNESINNNNTLNNNNEYNNQNNNEDEDDDDWGELGEDKYIDINSIMKKKNVI 499
Query: 71 IQQV 74
+ Q+
Sbjct: 500 LNQL 503
Score = 28.1 bits (61), Expect = 3.5
Identities = 22/114 (19%), Positives = 46/114 (40%), Gaps = 19/114 (16%)
Query: 15 NHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQV 74
N + N +R + NN+ + + G + Q N +F + +R ++ +
Sbjct: 217 NRNNSINIKRNLNNNNNINNNMNKM-----GSQDKNQNSNNNFYMNYNYQNRKNSMNNNM 271
Query: 75 NENQQSRMPDNMVKNVG--------------LIQELNGNISKVASLYSDLNSDF 114
N N + M NM N+ + +N N++ + SL SD++ ++
Sbjct: 272 NNNMNNNMNHNMNNNMNHNMNNNMNHNMNNNMNHNMNNNMNNINSLDSDMSPNY 325
>S155_YEAST (P43612) SIT4-associating protein SAP155
Length = 1000
Score = 29.6 bits (65), Expect = 1.2
Identities = 25/87 (28%), Positives = 44/87 (49%), Gaps = 20/87 (22%)
Query: 12 EDTNHRSKKNQRRRRPSATPTNNDYS---DVEDAEDGDPEAWQT---------------L 53
+D N +KK RR+R S++ N+D + D +DA + D A+ T +
Sbjct: 239 DDNNSNNKK--RRKRGSSSFGNDDINNNDDDDDANEDDESAYLTKATIISEIFSLDIWLI 296
Query: 54 NKSFRQVQSVLDRNRAIIQQVNENQQS 80
++S + QS L++ +II Q N N ++
Sbjct: 297 SESLVKNQSYLNKIWSIINQPNFNSEN 323
>RS20_WIGBR (Q8D2Q8) 30S ribosomal protein S20
Length = 88
Score = 29.6 bits (65), Expect = 1.2
Identities = 19/84 (22%), Positives = 48/84 (56%), Gaps = 2/84 (2%)
Query: 11 MEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGD-PEAWQTLNKSFRQVQSVLDRNRA 69
++ + RS +++++R+ +++ + S ++ ++ + T+N SF ++QS+LDR A
Sbjct: 4 IKSSKKRSIQSEKKRKYNSSKKSMIRSFIKKVKNSILKKEKDTINNSFIEMQSILDR-YA 62
Query: 70 IIQQVNENQQSRMPDNMVKNVGLI 93
+++N+ SR+ + K + I
Sbjct: 63 KKNLIHKNKASRLKSRLSKKIKFI 86
>MSS4_YEAST (P38994) Probable phosphatidylinositol-4-phosphate
5-kinase MSS4 (EC 2.7.1.68)
(1-phosphatidylinositol-4-phosphate kinase) (PIP5K)
(PtdIns(4)P-5-kinase) (Diphosphoinositide kinase)
Length = 779
Score = 29.6 bits (65), Expect = 1.2
Identities = 21/83 (25%), Positives = 38/83 (45%), Gaps = 6/83 (7%)
Query: 8 SSAMEDTNHRSKKNQRRRRPSATPTNNDYSDVE-----DAEDGDPEAWQTLNKSFRQVQS 62
S++ D+NHR KK+ R S ++N S++E D E P + + LN + +
Sbjct: 47 SNSNPDSNHRIKKD-RNNHTSYHSSSNSESNMESPRLSDGESSTPTSIEELNPTINNSRL 105
Query: 63 VLDRNRAIIQQVNENQQSRMPDN 85
V I +++N + D+
Sbjct: 106 VKRNYSISIDPLHDNSNNNTDDD 128
>C1CB_BACTG (P56953) Pesticidial crystal protein cry1Cb
(Insecticidal delta-endotoxin CryIC(b)) (Crystaline
entomocidal protoxin) (133 kDa crystal protein)
Length = 1176
Score = 29.6 bits (65), Expect = 1.2
Identities = 30/118 (25%), Positives = 53/118 (44%), Gaps = 18/118 (15%)
Query: 10 AMEDTNHRSKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRA 69
A+ KK + +R TN Y + +++ D +N + ++Q+ D N A
Sbjct: 870 ALARVKRAEKKWRDKREKLEWETNIVYKEAKESVDA-----LFVNSQYDRLQA--DTNIA 922
Query: 70 II----QQVNENQQSRMPDNMV---KNVGLIQELNGNISKVASLYSD----LNSDFTN 116
+I ++V+ +++ +P+ V N G+ +EL G I SLY N DF N
Sbjct: 923 MIHAADKRVHRIREAYLPELSVIPGVNAGIFEELEGRIFTAYSLYDARNVIKNGDFNN 980
>MC70_YEAST (Q12411) Sporulation-specific protein 21 (Meiotic plaque
component protein 70)
Length = 609
Score = 29.3 bits (64), Expect = 1.6
Identities = 27/122 (22%), Positives = 52/122 (42%), Gaps = 12/122 (9%)
Query: 18 SKKNQRRRRPSATPTNNDYSDVEDAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQQVNEN 77
S +N++ + N Y+ E+ +TL++ R+ Q ++D+N + + VNE
Sbjct: 325 SLENKKIKEAFKELDNESYNHDEELLKKYKYTRETLDRVNREQQLIIDQNEFLKKSVNEL 384
Query: 78 QQ---------SRMPDNMVKNVGLIQELNGNISKVASLYSDLN---SDFTNICHQQQRSS 125
Q S + K I ELN + K +L +L ++ IC + +++
Sbjct: 385 QNEVNATNFKFSLFKEKYAKLADSITELNTSTKKREALGENLTFECNELKEICLKYKKNI 444
Query: 126 HN 127
N
Sbjct: 445 EN 446
>COAE_STAAW (P63832) Dephospho-CoA kinase (EC 2.7.1.24)
(Dephosphocoenzyme A kinase)
Length = 207
Score = 29.3 bits (64), Expect = 1.6
Identities = 16/71 (22%), Positives = 35/71 (48%), Gaps = 8/71 (11%)
Query: 41 DAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQ--------QVNENQQSRMPDNMVKNVGL 92
+ E+ E W Q+ ++ RN ++ Q++ +++SRM D+++ N+G
Sbjct: 121 ELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYSQISIDKKSRMADHVIDNLGD 180
Query: 93 IQELNGNISKV 103
EL N+ ++
Sbjct: 181 KLELKQNLERL 191
>COAE_STAAN (P63831) Dephospho-CoA kinase (EC 2.7.1.24)
(Dephosphocoenzyme A kinase)
Length = 207
Score = 29.3 bits (64), Expect = 1.6
Identities = 16/71 (22%), Positives = 35/71 (48%), Gaps = 8/71 (11%)
Query: 41 DAEDGDPEAWQTLNKSFRQVQSVLDRNRAIIQ--------QVNENQQSRMPDNMVKNVGL 92
+ E+ E W Q+ ++ RN ++ Q++ +++SRM D+++ N+G
Sbjct: 121 ELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYSQISIDKKSRMADHVIDNLGD 180
Query: 93 IQELNGNISKV 103
EL N+ ++
Sbjct: 181 KLELKQNLERL 191
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.309 0.123 0.340
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,215,607
Number of Sequences: 164201
Number of extensions: 571549
Number of successful extensions: 1819
Number of sequences better than 10.0: 70
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 47
Number of HSP's that attempted gapping in prelim test: 1790
Number of HSP's gapped (non-prelim): 74
length of query: 130
length of database: 59,974,054
effective HSP length: 106
effective length of query: 24
effective length of database: 42,568,748
effective search space: 1021649952
effective search space used: 1021649952
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC145219.5