BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144807.5 + phase: 0
(112 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BFS2_HUMAN (Q13515) Phakinin (Beaded filament structural protein... 30 1.3
RPB1_ARATH (P18616) DNA-directed RNA polymerase II largest subun... 29 1.7
PBPA_ECOLI (P02918) Penicillin-binding protein 1A (PBP-1a) (PBP1... 29 1.7
VIME_PIG (P02543) Vimentin (Fragments) 29 2.2
VIME_CHICK (P09654) Vimentin 29 2.2
VIME_RAT (P31000) Vimentin 28 2.9
VIME_MOUSE (P20152) Vimentin 28 2.9
VIME_MESAU (P02544) Vimentin 28 2.9
VIME_HUMAN (P08670) Vimentin 28 2.9
VIME_CRIGR (P48670) Vimentin (Fragment) 28 2.9
VIME_BOVIN (P48616) Vimentin 28 2.9
VIM2_CARAU (P48672) Vimentin A2 (Fragment) 28 2.9
VG20_BPMV4 (Q04764) Minor structural protein GP20 28 2.9
PFD4_HUMAN (Q9NQP4) Prefoldin subunit 4 (Protein C-1) 28 2.9
GUND_CLOTM (P04954) Endoglucanase D precursor (EC 3.2.1.4) (EGD)... 28 2.9
CN04_MOUSE (Q8K3X4) Protein C14orf4 homolog 28 2.9
YL15_MYCLE (P46509) Putative AAA-family ATPase ML1316 28 3.8
LIPA_CAEEL (Q21049) Liprin alpha (LAR-interacting protein alpha)... 28 3.8
HRCA_BACAN (Q81LS0) Heat-inducible transcription repressor hrcA 28 3.8
YIAO_HAEIN (P44992) Protein HI1028 precursor 28 4.9
>BFS2_HUMAN (Q13515) Phakinin (Beaded filament structural protein 2)
(Lens fiber cell beaded filament protein CP 49) (CP49)
(49 kDa cytoskeletal protein) (CP 47) (CP47) (Lens
intermediate filament like-light) (LIFL-L)
Length = 415
Score = 29.6 bits (65), Expect = 1.3
Identities = 24/72 (33%), Positives = 33/72 (45%), Gaps = 2/72 (2%)
Query: 30 AILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLWDWKQQLYAEAASDSAPVPL--ARL 87
A L +N +++L+AE +SL + + NTL D K E + A V A L
Sbjct: 315 AALRVELHNTSCQVQSLQAETESLRALKRGLENTLHDAKHWHDMELQNLGAVVGRLEAEL 374
Query: 88 YEIYGEAAPPQQ 99
EI EA QQ
Sbjct: 375 REIRAEAEQQQQ 386
>RPB1_ARATH (P18616) DNA-directed RNA polymerase II largest subunit
(EC 2.7.7.6)
Length = 1840
Score = 29.3 bits (64), Expect = 1.7
Identities = 16/51 (31%), Positives = 30/51 (58%), Gaps = 3/51 (5%)
Query: 37 YNQQKIIEALEAEKDSLTKTNKSIRNTLWDWKQQLYAEAASDSAPVPLARL 87
Y Q+++++A+E D + K + ++RN+L D Q LY E D+ + +L
Sbjct: 856 YIQRRLVKAME---DIMVKYDGTVRNSLGDVIQFLYGEDGMDAVWIESQKL 903
>PBPA_ECOLI (P02918) Penicillin-binding protein 1A (PBP-1a) (PBP1a)
[Includes: Penicillin-insensitive transglycosylase (EC
2.4.2.-) (Peptidoglycan TGase); Penicillin-sensitive
transpeptidase (EC 3.4.-.-) (DD-transpeptidase)]
Length = 850
Score = 29.3 bits (64), Expect = 1.7
Identities = 24/82 (29%), Positives = 35/82 (42%), Gaps = 4/82 (4%)
Query: 21 INVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLWDWKQQLYAEAASDSA 80
IN GAV A++G +NQ K A +A L + +I+ L+ AS
Sbjct: 432 INPQNGAVMALVGGFDFNQSKFNRATQA----LRQVGSNIKPFLYTAAMDKGLTLASMLN 487
Query: 81 PVPLARLYEIYGEAAPPQQSAP 102
VP++R G P+ S P
Sbjct: 488 DVPISRWDASAGSDWQPKNSPP 509
>VIME_PIG (P02543) Vimentin (Fragments)
Length = 275
Score = 28.9 bits (63), Expect = 2.2
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A + Y +Q +++L E D+L TN+S+ +
Sbjct: 103 KFADLSEAANRNNDALRQ------AKQESNEYRRQ--VQSLTCEVDALKGTNESLERQMR 154
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 155 EMEENFAVEAAN 166
>VIME_CHICK (P09654) Vimentin
Length = 459
Score = 28.9 bits (63), Expect = 2.2
Identities = 19/72 (26%), Positives = 36/72 (49%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A A Y +Q I++L E D+L +N+S+ +
Sbjct: 287 KFADLSEAANRNNDALRQ------AKQEANEYRRQ--IQSLTCEVDALKGSNESLERQMR 338
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 339 EMEENFAVEAAN 350
>VIME_RAT (P31000) Vimentin
Length = 465
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A + Y +Q +++L E D+L TN+S+ +
Sbjct: 293 KFADLSEAANRNNDALRQ------AKQESNEYRRQ--VQSLTCEVDALKGTNESLERQMR 344
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 345 EMEENFALEAAN 356
>VIME_MOUSE (P20152) Vimentin
Length = 465
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A + Y +Q +++L E D+L TN+S+ +
Sbjct: 293 KFADLSEAANRNNDALRQ------AKQESNEYRRQ--VQSLTCEVDALKGTNESLERQMR 344
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 345 EMEENFALEAAN 356
>VIME_MESAU (P02544) Vimentin
Length = 464
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A + Y +Q +++L E D+L TN+S+ +
Sbjct: 292 KFADLSEAANRNNDALRQ------AKQESNEYRRQ--VQSLTCEVDALKGTNESLERQMR 343
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 344 EMEENFALEAAN 355
>VIME_HUMAN (P08670) Vimentin
Length = 465
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A + Y +Q +++L E D+L TN+S+ +
Sbjct: 293 KFADLSEAANRNNDALRQ------AKQESTEYRRQ--VQSLTCEVDALKGTNESLERQMR 344
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 345 EMEENFAVEAAN 356
>VIME_CRIGR (P48670) Vimentin (Fragment)
Length = 448
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/72 (25%), Positives = 36/72 (50%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A + Y +Q +++L E D+L TN+S+ +
Sbjct: 276 KFADLSEAANRNNDALRQ------AKQESNEYRRQ--VQSLTCEVDALKGTNESLERQMR 327
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 328 EMEENFALEAAN 339
>VIME_BOVIN (P48616) Vimentin
Length = 465
Score = 28.5 bits (62), Expect = 2.9
Identities = 18/72 (25%), Positives = 35/72 (48%), Gaps = 8/72 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+++ A+ NN + A + Y +Q ++ L E D+L TN+S+ +
Sbjct: 293 KFADLSEAANRNNDALRQ------AKQESNEYRRQ--VQTLTCEVDALKGTNESLERQMR 344
Query: 66 DWKQQLYAEAAS 77
+ ++ EAA+
Sbjct: 345 EMEENFSVEAAN 356
>VIM2_CARAU (P48672) Vimentin A2 (Fragment)
Length = 243
Score = 28.5 bits (62), Expect = 2.9
Identities = 23/84 (27%), Positives = 42/84 (49%), Gaps = 10/84 (11%)
Query: 6 KIVDIAKRASENNTVINVGLGAVFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLW 65
K D+ + A+++N + + A A Y +Q ++AL E D+L TN+S+ +
Sbjct: 71 KFADMTEAANKSNEALRL------AKQEANEYRRQ--VQALTCEVDALKGTNESLERQMR 122
Query: 66 DWKQQLYAEAASDSAPVPLARLYE 89
+ ++ E S S+ +ARL E
Sbjct: 123 EIEENFAIE--SSSSQDNIARLEE 144
>VG20_BPMV4 (Q04764) Minor structural protein GP20
Length = 180
Score = 28.5 bits (62), Expect = 2.9
Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Query: 16 ENNTVINVGLGA--VFAILGARSYNQQKIIEALEAEKDSLTKTNKSIRNTLWDWK 68
E + ++GL V +I+ + QK E LEAE+D+L KT+ + N + D K
Sbjct: 2 ERKFLTDLGLNPDQVNSIMAQYGKDMQKY-EGLEAERDALKKTSSELSNKIEDLK 55
>PFD4_HUMAN (Q9NQP4) Prefoldin subunit 4 (Protein C-1)
Length = 134
Score = 28.5 bits (62), Expect = 2.9
Identities = 23/68 (33%), Positives = 37/68 (53%), Gaps = 4/68 (5%)
Query: 14 ASENNTVINVGLGAVFAILGARSYNQQKIIEA---LEAEKDSLTKTNKSIRNTLWDWKQQ 70
A ++ +I +G VF I ++ Q+ + EA L+ E D+L +SI+ L D K Q
Sbjct: 60 ADDDCLMIPYQIGDVF-ISHSQEETQEMLEEAKKNLQEEIDALESRVESIQRVLADLKVQ 118
Query: 71 LYAEAASD 78
LYA+ S+
Sbjct: 119 LYAKFGSN 126
>GUND_CLOTM (P04954) Endoglucanase D precursor (EC 3.2.1.4) (EGD)
(Endo-1,4-beta-glucanase) (Cellulase D)
Length = 649
Score = 28.5 bits (62), Expect = 2.9
Identities = 11/36 (30%), Positives = 19/36 (52%)
Query: 7 IVDIAKRASENNTVINVGLGAVFAILGARSYNQQKI 42
I+ +A + S NN +N L A+ + G YN+ +
Sbjct: 469 ILQVANKISPNNDYVNAALDAISHVFGRNYYNRSYV 504
>CN04_MOUSE (Q8K3X4) Protein C14orf4 homolog
Length = 775
Score = 28.5 bits (62), Expect = 2.9
Identities = 15/55 (27%), Positives = 33/55 (59%), Gaps = 1/55 (1%)
Query: 14 ASENNTVINVGLGAVFAILGA-RSYNQQKIIEALEAEKDSLTKTNKSIRNTLWDW 67
+S ++TV VG+GA G+ S +Q++ ++ + ++L + ++S+RN +W
Sbjct: 293 SSTSSTVAEVGVGAAGKRPGSVSSTDQERELKEKQRNAEALAELSESLRNRAEEW 347
>YL15_MYCLE (P46509) Putative AAA-family ATPase ML1316
Length = 609
Score = 28.1 bits (61), Expect = 3.8
Identities = 14/31 (45%), Positives = 19/31 (61%)
Query: 43 IEALEAEKDSLTKTNKSIRNTLWDWKQQLYA 73
+ LEA DSLT N + +TL + +QQL A
Sbjct: 56 VHQLEARIDSLTARNSKLMDTLKEARQQLLA 86
>LIPA_CAEEL (Q21049) Liprin alpha (LAR-interacting protein alpha)
(Synapse defective protein 2)
Length = 1139
Score = 28.1 bits (61), Expect = 3.8
Identities = 22/69 (31%), Positives = 33/69 (46%), Gaps = 7/69 (10%)
Query: 40 QKIIEALEAEKDSLTKTNKSIRNTLWDWKQQLY----AEAASDSAPVPLARLYEI---YG 92
+KI + E KD L + N+S+R + +QQLY A+ S +P I
Sbjct: 477 KKIYDQAERIKDRLQRDNESLRQEIEALRQQLYNARTAQFQSRMHAIPFTHAQNIVQQQP 536
Query: 93 EAAPPQQSA 101
+A+ QQSA
Sbjct: 537 QASIAQQSA 545
>HRCA_BACAN (Q81LS0) Heat-inducible transcription repressor hrcA
Length = 338
Score = 28.1 bits (61), Expect = 3.8
Identities = 17/58 (29%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
Query: 48 AEKDSLTKTNKSIRNTLWDWKQQLYAEAA-SDSAPVPLARLYEIYGEAAPPQQSAPGN 104
A+KD +T ++ +IRN + D ++ + E S S VP + Y Y + Q+ P +
Sbjct: 30 AKKDEITYSSATIRNEMADLEELGFIEKTHSSSGRVPSEKGYRFYVDHLLAPQNLPND 87
>YIAO_HAEIN (P44992) Protein HI1028 precursor
Length = 328
Score = 27.7 bits (60), Expect = 4.9
Identities = 30/113 (26%), Positives = 49/113 (42%), Gaps = 16/113 (14%)
Query: 2 EFVHKIVDIAKRASENNTVINVGL-GAVFAILGARSY-NQQKIIEALEAEKDSLTKTNKS 59
E V+K++D + + GL G F +G R++ N ++ + E K +TN++
Sbjct: 115 EHVYKVLDGEVGQNLLKDLEKQGLKGLAFWDVGFRAFSNSKQTVTKPEHIKGLKVRTNQN 174
Query: 60 IRNTLWDWKQQLYAEA----ASDSAPVPLARLYEIYGEAAPPQQSAPGNLFFS 108
+Y EA S+ P+PLA LY A Q P +F+S
Sbjct: 175 ----------PMYIEAFKLLGSNPVPMPLAELYTALETRAVDAQEHPIGIFWS 217
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.131 0.369
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,881,947
Number of Sequences: 164201
Number of extensions: 399623
Number of successful extensions: 1417
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 28
Number of HSP's that attempted gapping in prelim test: 1405
Number of HSP's gapped (non-prelim): 40
length of query: 112
length of database: 59,974,054
effective HSP length: 88
effective length of query: 24
effective length of database: 45,524,366
effective search space: 1092584784
effective search space used: 1092584784
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144807.5