BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144806.12 - phase: 1 /pseudo
(316 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
MOC_ORYSA (Q84MM9) Protein MONOCULM 1 154 2e-37
SCP2_RAT (O70608) Synaptonemal complex protein 2 (SCP-2 protein)... 35 0.32
SCP2_MOUSE (Q9CUU3) Synaptonemal complex protein 2 (SCP-2 protei... 34 0.54
SNE2_HUMAN (Q8WXH0) Nesprin 2 (Nuclear envelope spectrin repeat ... 33 1.2
YCAL_ECOLI (P43674) Putative metalloprotease ycaL (EC 3.4.24.-) 32 1.6
SUBT_BACLI (P00780) Subtilisin Carlsberg precursor (EC 3.4.21.62) 31 3.5
THIE_BACCR (Q81IG8) Thiamine-phosphate pyrophosphorylase (EC 2.5... 31 4.6
4OMT_COPJA (Q9LEL5) 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-meth... 30 6.0
YL61_SCHPO (Q8TFG9) Hypothetical serine/threonine-rich protein P... 30 7.9
>MOC_ORYSA (Q84MM9) Protein MONOCULM 1
Length = 441
Score = 154 bits (390), Expect = 2e-37
Identities = 113/326 (34%), Positives = 161/326 (48%), Gaps = 50/326 (15%)
Query: 26 SYKALNDACPYSKFAHLTANQAILEATEGSNNIHIVDFGIVQGIQWAALLQAFATRSSGK 85
+Y A N P+ +FAHLTANQAILEA +G+ +HI+D V G+QW LLQA A R+
Sbjct: 126 AYLAFNQIAPFLRFAHLTANQAILEAVDGARRVHILDLDAVHGVQWPPLLQAIAERADPA 185
Query: 86 --PNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLGLNFEFTPIL------TPIELL 137
P VR++G A ++ TGNRL FA+ + L F FTP+L P +
Sbjct: 186 LGPPEVRVTGAGA------DRDTLLRTGNRLRAFARSIHLPFHFTPLLLSCATTAPHHVA 239
Query: 138 DESSFC--------------IQPDEALAVNFMLQLYNLLDENTNSVEKALRLAKSLNPKI 183
S+ PDE LAVN ++ L+NL + + L+ K+++P +
Sbjct: 240 GTSTGAAAAASTAAAATGLEFHPDETLAVNCVMFLHNLAGH--DELAAFLKWVKAMSPAV 297
Query: 184 VTLGEYEA-----------SLTTRVGFVERFETAFNYFAAFFESLEPNMALDSPERFQVE 232
VT+ E EA L RVG A ++++A FE+LE + S ER VE
Sbjct: 298 VTIAEREAGGGGGGGDHIDDLPRRVG------VAMDHYSAVFEALEATVPPGSRERLAVE 351
Query: 233 SLLLGRRIDGVIGVR--ERMEDKEQWKVLMENCGFESVGLSHYAISQAKILLWNYSYSSL 290
+LGR I+ +G E+W GF + LS +A+SQA++LL Y S
Sbjct: 352 QEVLGREIEAAVGPSGGRWWRGIERWGGAARAAGFAARPLSAFAVSQARLLL-RLHYPSE 410
Query: 291 YSLVESQPAFLSLAWKDVPLLTVSSW 316
LV+ L W+ PLL+VS+W
Sbjct: 411 GYLVQEARGACFLGWQTRPLLSVSAW 436
>SCP2_RAT (O70608) Synaptonemal complex protein 2 (SCP-2 protein)
(Synaptonemal complex lateral element protein) (rnSCP2)
Length = 1505
Score = 34.7 bits (78), Expect = 0.32
Identities = 31/137 (22%), Positives = 63/137 (45%), Gaps = 22/137 (16%)
Query: 127 FTPILTPIELLDESSFCIQPDEALAVNFMLQLYNLLDENTNSV---EKALRLAKSLNPKI 183
F P + + + +FCIQ + +N ML + + ++ N + ++ L L ++ +I
Sbjct: 151 FIPHICALVIDSRVNFCIQQEALKKMNLML---DRIPQDANKILCNQEILTLMSNMGERI 207
Query: 184 VTLGEYEASLTTRVGFVE----------RFETAFNYFAAFFESLEPNMALDSPERFQVES 233
+ +G+YE +VG VE R E A+ +F+ F + D F+ +
Sbjct: 208 LDVGDYE----LQVGIVEALCRMTTEKRRQELAYEWFSMDFIANAFKKIKDC--EFETDC 261
Query: 234 LLLGRRIDGVIGVRERM 250
+ ++G++G R R+
Sbjct: 262 RIFLNLVNGMLGDRRRV 278
>SCP2_MOUSE (Q9CUU3) Synaptonemal complex protein 2 (SCP-2 protein)
(Synaptonemal complex lateral element protein)
(Fragment)
Length = 557
Score = 33.9 bits (76), Expect = 0.54
Identities = 30/134 (22%), Positives = 58/134 (42%), Gaps = 16/134 (11%)
Query: 127 FTPILTPIELLDESSFCIQPDEALAVNFMLQLYNLLDENTNSVEKALRLAKSLNPKIVTL 186
F P + + + +FCIQ + +N ML S ++ L L ++ +I+ +
Sbjct: 151 FIPQICALVIDSRVNFCIQQEALKKMNLMLDRIPQDANKILSNQEMLTLMSNMGERILDV 210
Query: 187 GEYEASLTTRVGFVE----------RFETAFNYFAAFFESLEPNMALDSPERFQVESLLL 236
G+YE +VG VE R E A+ +F+ F + D F+ + +
Sbjct: 211 GDYE----LQVGIVEALCRMTTEKRRQELAYEWFSMDFIANAFKEIKDC--EFETDCRIF 264
Query: 237 GRRIDGVIGVRERM 250
++G++G + R+
Sbjct: 265 LNLVNGILGDKRRV 278
>SNE2_HUMAN (Q8WXH0) Nesprin 2 (Nuclear envelope spectrin repeat
protein 2) (Syne-2) (Synaptic nuclear envelope protein 2)
(Nucleus and actin connecting element protein) (NUANCE
protein)
Length = 6885
Score = 32.7 bits (73), Expect = 1.2
Identities = 20/74 (27%), Positives = 37/74 (49%), Gaps = 4/74 (5%)
Query: 131 LTPIELLDESSFCIQPDEALAVNFMLQLYNLLDENTNSVEKALRLAKSLNPKIVTLGEYE 190
+T +++L+E S + + +LQ + DEN S+EK L + S P + + E E
Sbjct: 4816 VTEVKILEEKS----RQCGMKLQSLLQKWEEFDENYASLEKDLEILISTLPSVSLVEETE 4871
Query: 191 ASLTTRVGFVERFE 204
L R+ F ++ +
Sbjct: 4872 ERLVERISFYQQIK 4885
>YCAL_ECOLI (P43674) Putative metalloprotease ycaL (EC 3.4.24.-)
Length = 254
Score = 32.3 bits (72), Expect = 1.6
Identities = 25/79 (31%), Positives = 34/79 (42%), Gaps = 6/79 (7%)
Query: 54 GSNNIHIVDFGIVQGIQWAALLQAF--ATRSSGKPNSVRISGIPAMALGTSPVSSISATG 111
G N H GI + ++ L A+ AT S ++ G M G S S G
Sbjct: 19 GCQNTH----GIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYG 74
Query: 112 NRLSEFAKLLGLNFEFTPI 130
RL++ AK LG N TP+
Sbjct: 75 KRLAKIAKALGNNINGTPV 93
>SUBT_BACLI (P00780) Subtilisin Carlsberg precursor (EC 3.4.21.62)
Length = 379
Score = 31.2 bits (69), Expect = 3.5
Identities = 28/126 (22%), Positives = 50/126 (39%), Gaps = 4/126 (3%)
Query: 10 IASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNNIHIVDFGIVQGI 69
+A++ +S ++ T+ Y A D+ +N+A + + G+
Sbjct: 254 VAAAGNSGSSGNTNTIGYPAKYDSVIAVGAVDSNSNRASFSSVGAELEVMAPGAGVYSTY 313
Query: 70 QWAALLQAFATRSSGKPNSVRISGIPAMALGTSPVSSISATGNRLSEFAKLLGLNFEFTP 129
+ +AT + S ++G A+ L P S S NRLS A LG +F +
Sbjct: 314 PTST----YATLNGTSMASPHVAGAAALILSKHPNLSASQVRNRLSSTATYLGSSFYYGK 369
Query: 130 ILTPIE 135
L +E
Sbjct: 370 GLINVE 375
>THIE_BACCR (Q81IG8) Thiamine-phosphate pyrophosphorylase (EC
2.5.1.3) (TMP pyrophosphorylase) (TMP-PPase)
(Thiamine-phosphate synthase)
Length = 219
Score = 30.8 bits (68), Expect = 4.6
Identities = 33/137 (24%), Positives = 61/137 (44%), Gaps = 25/137 (18%)
Query: 155 MLQLYNLLDENTNSVEKALRLAKSLNPKIVTLGEY----EASLT--TRVGFVERFETAFN 208
+LQ+Y ++ N N L + K VT+ ++ E SL RV F + +T N
Sbjct: 12 LLQVYFIMGSN-NCTRDPLAVLKEALDGGVTIFQFREKGEGSLIGEDRVRFAKELQTLCN 70
Query: 209 -YFAAFFESLEPNMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMENCGFES 267
Y F + + +A++ + + +G+ +G+ VRE+M DK
Sbjct: 71 EYSVPFIVNDDVELAIE----LDADGVHVGQDDEGITSVREKMGDK-------------I 113
Query: 268 VGLSHYAISQAKILLWN 284
+G+S + I +A+ + N
Sbjct: 114 IGVSAHTIEEARFAIEN 130
>4OMT_COPJA (Q9LEL5) 3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase (EC 2.1.1.116)
(S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase) (4'-OMT)
Length = 350
Score = 30.4 bits (67), Expect = 6.0
Identities = 16/61 (26%), Positives = 30/61 (48%)
Query: 220 NMALDSPERFQVESLLLGRRIDGVIGVRERMEDKEQWKVLMENCGFESVGLSHYAISQAK 279
++ALD ++ S L ID ++ + KE W+ ++++ GF + H A Q+
Sbjct: 286 DVALDEESDHELSSTRLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSV 345
Query: 280 I 280
I
Sbjct: 346 I 346
>YL61_SCHPO (Q8TFG9) Hypothetical serine/threonine-rich protein
PB15E9.01c in chromosome I precursor
Length = 943
Score = 30.0 bits (66), Expect = 7.9
Identities = 30/126 (23%), Positives = 58/126 (45%), Gaps = 8/126 (6%)
Query: 1 TNKITAQSSIASSNSSSTTWEELTLSYKALNDACPYSKFAHLTANQAILEATEGSNNIHI 60
++ +T+ SS+ASS+++STT T S + A S + T + ++ ++ S+++
Sbjct: 82 SSSLTSSSSLASSSTNSTTSASPTSSSLTSSSATSSSLASSSTTSSSLASSSITSSSL-- 139
Query: 61 VDFGIVQGIQWAALLQAFATRSSGKPNSVR--ISGIPAMALGTSPVSSISATGNRLSEFA 118
I ++L + T SS +S S P + +S +SS +A+ + S
Sbjct: 140 ----ASSSITSSSLASSSTTSSSLASSSTNSTTSATPTSSATSSSLSSTAASNSATSSSL 195
Query: 119 KLLGLN 124
LN
Sbjct: 196 ASSSLN 201
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.316 0.131 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 33,665,294
Number of Sequences: 164201
Number of extensions: 1321703
Number of successful extensions: 3788
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3777
Number of HSP's gapped (non-prelim): 10
length of query: 316
length of database: 59,974,054
effective HSP length: 110
effective length of query: 206
effective length of database: 41,911,944
effective search space: 8633860464
effective search space used: 8633860464
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 66 (30.0 bits)
Medicago: description of AC144806.12