BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.9 + phase: 0
(273 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YG3X_YEAST (P53294) Hypothetical 46.8 kDa protein in CLC1-PDS2 i... 35 0.15
Y242_MYCGE (P47484) Hypothetical protein MG242 33 0.57
MYOB_TOXGO (O00936) Myosin B/C (MyoB/C) 33 0.74
VS06_ROTE1 (P16592) VP6 protein (Major capsid protein) (Major in... 32 1.7
PGTA_MOUSE (Q9JHK4) Geranylgeranyl transferase type II alpha sub... 31 2.8
PCRA_MYCLE (Q9CD72) ATP-dependent DNA helicase pcrA (EC 3.6.1.-) 31 3.7
YON1_SCHPO (Q9Y824) Hypothetical protein C1105.01 in chromosome II 30 4.8
PGTA_RAT (Q08602) Geranylgeranyl transferase type II alpha subun... 30 4.8
SC17_SCHPO (Q9P4X4) Probable vesicular-fusion protein sec17 homolog 30 6.3
YD61_YEAST (P38961) Hypothetical 47.2 kDa protein in STN1-AFR1 i... 30 8.2
>YG3X_YEAST (P53294) Hypothetical 46.8 kDa protein in CLC1-PDS2
intergenic region
Length = 404
Score = 35.4 bits (80), Expect = 0.15
Identities = 26/74 (35%), Positives = 34/74 (45%), Gaps = 11/74 (14%)
Query: 43 PGWANSHDLYAIE-----QKFMENCALNYFDKNDYRSMMKFVRAFHSIDLKRGFLQSLNL 97
P W N DL A E QKF++ KN+ R+M F H++DLK L L L
Sbjct: 322 PRWENQQDLDAEELKVRFQKFVDET------KNNCRTMETFCPECHTVDLKDPVLSDLEL 375
Query: 98 PDELLELEEESGNF 111
+ EE +G F
Sbjct: 376 WLHAWKYEEINGKF 389
>Y242_MYCGE (P47484) Hypothetical protein MG242
Length = 630
Score = 33.5 bits (75), Expect = 0.57
Identities = 23/81 (28%), Positives = 40/81 (48%), Gaps = 5/81 (6%)
Query: 36 KQNERADPGWANSHDLYA----IEQKFMENCALNYFDKNDYRSMMKFVRAFHSIDLKRGF 91
K R DP DLY IEQ +++ ++K + + + + + I+L+
Sbjct: 291 KNTLREDPDQNPETDLYELIQFIEQNYLKKDKKTSWNKKKVQDLEQLLEEINKINLETKN 350
Query: 92 LQSLNLPDELLELEEESGNFM 112
+SL PDE+ ELE ++ NF+
Sbjct: 351 -ESLAYPDEITELEIDNDNFV 370
>MYOB_TOXGO (O00936) Myosin B/C (MyoB/C)
Length = 1171
Score = 33.1 bits (74), Expect = 0.74
Identities = 30/103 (29%), Positives = 44/103 (42%), Gaps = 4/103 (3%)
Query: 22 GGLLDTGSHYIQCWKQNERADP-GWANSHDLYAI-EQKFMENCALNYFDKNDYRSMMKFV 79
G + T +H+I+C K NE P GW S L + +E L R+ +F
Sbjct: 664 GIVAQTEAHFIRCLKPNEEKKPLGWNGSKVLNQLFSLSILEALQLRQVGYAYRRNFSEFC 723
Query: 80 RAFHSIDLKRGFLQSLNLPDELLELEEESGNFMEAAVNIAKTM 122
F +DL G + S E+ +L E E++ I KTM
Sbjct: 724 SHFRWLDL--GLVNSDRDRKEVAQLLLEQSGIPESSWVIGKTM 764
>VS06_ROTE1 (P16592) VP6 protein (Major capsid protein) (Major
internal structural protein)
Length = 397
Score = 32.0 bits (71), Expect = 1.7
Identities = 21/89 (23%), Positives = 39/89 (43%), Gaps = 6/89 (6%)
Query: 112 MEAAVNIAKTMGDI---LREADLLGKAGEFLDAYELVFFYVFAKSLWSGGSKAWPLKQFT 168
ME +I+KT+ D + E L + + + + + +GG P++ +T
Sbjct: 1 MEVLYSISKTLKDARDKIVEGTLYSNVSDIIQQFNQIIVTMNGNEFQTGGIGTLPIRNWT 60
Query: 169 QKAGLLGKALTFAKEVSSSFYELASTKVE 197
GLLG L + +++ E A T +E
Sbjct: 61 FDFGLLGTTLL---NLDANYVETARTTIE 86
>PGTA_MOUSE (Q9JHK4) Geranylgeranyl transferase type II alpha
subunit (EC 2.5.1.60) (Rab geranylgeranyltransferase
alpha subunit) (Rab geranyl-geranyltransferase alpha
subunit) (Rab GG transferase alpha) (Rab GGTase alpha)
Length = 567
Score = 31.2 bits (69), Expect = 2.8
Identities = 27/81 (33%), Positives = 37/81 (45%), Gaps = 11/81 (13%)
Query: 1 MAAQVYARGSFFSDCLNVCAKGGLLDTGSHYIQCWKQNERADPGWANSHDLYAIEQKFME 60
+AA V A F CL V K G+ + +CW + +P WA +L A +F+E
Sbjct: 85 LAALVKAELGFLESCLRVNPKS----YGTWHHRCWLLSRLPEPNWARELELCA---RFLE 137
Query: 61 NCALNYFDKNDYRSMMKFVRA 81
N F DYR +FV A
Sbjct: 138 ADERN-FHCWDYR---RFVAA 154
>PCRA_MYCLE (Q9CD72) ATP-dependent DNA helicase pcrA (EC 3.6.1.-)
Length = 778
Score = 30.8 bits (68), Expect = 3.7
Identities = 20/90 (22%), Positives = 42/90 (46%), Gaps = 5/90 (5%)
Query: 61 NCALNYFDKNDYRSMMKFVRAFHSIDLKRGFLQSL-----NLPDELLELEEESGNFMEAA 115
N + +D +D RS+++ + +D+KR + + NL +EL++ E N
Sbjct: 121 NSNFSIYDVDDSRSLLQMIGQDMGLDIKRYSPRLMANAISNLKNELIDAESAVANLSSDT 180
Query: 116 VNIAKTMGDILREADLLGKAGEFLDAYELV 145
++A+T+ + E + LD +L+
Sbjct: 181 DDLARTVATVYGEYQRRLRTANALDFDDLI 210
>YON1_SCHPO (Q9Y824) Hypothetical protein C1105.01 in chromosome II
Length = 1001
Score = 30.4 bits (67), Expect = 4.8
Identities = 38/152 (25%), Positives = 69/152 (45%), Gaps = 25/152 (16%)
Query: 77 KFVRAFHSIDLKRGFLQSLNLPDELLELEEESGNFMEAAVNIAKT--MGDILREADLLGK 134
K V +DL + F+Q L P L E F + NI K+ + ++ R+ ++ G+
Sbjct: 423 KLVETPECLDLFKIFIQKLASPTFL-----ECLGFADNFCNIQKSHWLPEVFRD-NITGE 476
Query: 135 AGEFLDAYELVFFYVFAKSLWSGGSKAWPLKQFTQKAGLLGKAL--TFAKEVSSSFY--- 189
E FF F ++ +G S+ W L KAG + L K+++S+ Y
Sbjct: 477 TPE-------TFFDCFLPTIEAGNSQWWELLPLYTKAGCITNNLNENTLKKLASAIYNDR 529
Query: 190 ----ELASTKVELSNKHDNIFEIVNQLKSSRI 217
+A V+L+ K +N+ ++++ S+ I
Sbjct: 530 SCHSHVAQAFVDLT-KIENVRQLISSNSSNLI 560
>PGTA_RAT (Q08602) Geranylgeranyl transferase type II alpha subunit
(EC 2.5.1.60) (Rab geranylgeranyltransferase alpha
subunit) (Rab geranyl-geranyltransferase alpha subunit)
(Rab GG transferase alpha) (Rab GGTase alpha)
Length = 567
Score = 30.4 bits (67), Expect = 4.8
Identities = 27/80 (33%), Positives = 36/80 (44%), Gaps = 11/80 (13%)
Query: 2 AAQVYARGSFFSDCLNVCAKGGLLDTGSHYIQCWKQNERADPGWANSHDLYAIEQKFMEN 61
AA V A F CL V K G+ + +CW + +P WA +L A +F+E
Sbjct: 86 AALVKAELGFLESCLRVNPKS----YGTWHHRCWLLSRLPEPNWARELELCA---RFLEA 138
Query: 62 CALNYFDKNDYRSMMKFVRA 81
N F DYR +FV A
Sbjct: 139 DERN-FHCWDYR---RFVAA 154
>SC17_SCHPO (Q9P4X4) Probable vesicular-fusion protein sec17 homolog
Length = 289
Score = 30.0 bits (66), Expect = 6.3
Identities = 36/148 (24%), Positives = 57/148 (38%), Gaps = 16/148 (10%)
Query: 26 DTGSHYIQCWKQNERADPGWANSHDLYAIEQKFMENCALNYFDKNDYRSMMKFVRAFHSI 85
D S Y++ +K R P A AIE + + ++R A + +
Sbjct: 73 DAASTYVEAFKSYRREKPSEAARVLQIAIEL---------FTRRGNFRRA-----ANYKM 118
Query: 86 DLKRGFLQSLNLPDELLELEEESGNFMEAAVNIAKTMGDILREADLLGKAGEFLDAYELV 145
DL F Q L L E++G + + A L+ ADL G GE+ + +
Sbjct: 119 DLGDIFEQELQDTKAALGAYEDAGEWYSSDQADALANKAYLKAADLAGLCGEY--SLAIR 176
Query: 146 FFYVFAKSLWSGGSKAWPLKQFTQKAGL 173
F A++ W +K + KAGL
Sbjct: 177 KFEQVARASVQNNLLKWSVKDYLLKAGL 204
>YD61_YEAST (P38961) Hypothetical 47.2 kDa protein in STN1-AFR1
intergenic region
Length = 402
Score = 29.6 bits (65), Expect = 8.2
Identities = 40/166 (24%), Positives = 68/166 (40%), Gaps = 25/166 (15%)
Query: 64 LNYFDKNDYRSMMKFVRA---FHSIDLKRGF-------LQSLNLPDE--------LLELE 105
+N F KN + K+++ HS DLK+ ++++ LPDE L +
Sbjct: 236 INNFFKNYNKKAKKYLKRRHKVHSFDLKKANERITVADIRNVPLPDESCTIVVFCLALMG 295
Query: 106 EESGNFMEAAVNIAKTMGDI----LREADLLGKAGEFLDAYELVFFYVFAKSLWSGGSKA 161
+F++ A I G++ ++ GK EF+DA +L+ F F K +
Sbjct: 296 TNFLDFIKEAYRILAPRGELWIAEIKSRFSDGKGNEFVDALKLMGF--FHKKTFDENKMF 353
Query: 162 WPLKQFTQKAGLLGKALTFAKEVSSSFYELASTKVELSNKHDNIFE 207
+ F A ++ + E F E+ + K EL K I E
Sbjct: 354 TRFEFFKPPAEIIEERRQ-KLERRQKFIEVETEKEELEKKRRKIAE 398
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.322 0.135 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,962,849
Number of Sequences: 164201
Number of extensions: 1192883
Number of successful extensions: 2950
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2949
Number of HSP's gapped (non-prelim): 10
length of query: 273
length of database: 59,974,054
effective HSP length: 109
effective length of query: 164
effective length of database: 42,076,145
effective search space: 6900487780
effective search space used: 6900487780
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 65 (29.6 bits)
Medicago: description of AC144765.9