BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144765.13 + phase: 0
(146 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
DYHG_CHLRE (Q39575) Dynein gamma chain, flagellar outer arm 31 1.1
HS70_THEAN (P16019) Heat shock 70 kDa protein (HSP 70.1) 30 1.4
GLMS_SALTY (Q8ZKX1) Glucosamine--fructose-6-phosphate aminotrans... 30 2.4
GLMS_SALTI (Q8Z2Q2) Glucosamine--fructose-6-phosphate aminotrans... 30 2.4
GLMS_ECOLI (P17169) Glucosamine--fructose-6-phosphate aminotrans... 30 2.4
GLMS_ECOL6 (Q8FBT4) Glucosamine--fructose-6-phosphate aminotrans... 30 2.4
GLMS_ECO57 (Q8XEG2) Glucosamine--fructose-6-phosphate aminotrans... 30 2.4
GC34_CAEEL (P92006) Soluble guanylate cyclase gcy-34 (EC 4.6.1.2) 30 2.4
YNV7_YEAST (P40152) Hypothetical 37.2 kDa protein in ALG9-RAP1 i... 29 3.1
YE74_METJA (Q58869) Hypothetical UPF0129 protein MJ1474 29 3.1
VP5_AHSV4 (Q02168) Outer capsid protein VP5 29 4.1
CAPP_STRA5 (Q8E0H2) Phosphoenolpyruvate carboxylase (EC 4.1.1.31... 29 4.1
CAPP_STRA3 (Q8E647) Phosphoenolpyruvate carboxylase (EC 4.1.1.31... 29 4.1
HEM3_PROMI (Q59684) Porphobilinogen deaminase (EC 2.5.1.61) (PBG... 28 5.3
GUNA_CALSA (P22534) Endoglucanase A precursor (EC 3.2.1.4) (Endo... 28 5.3
YFDQ_ECOLI (P76513) Hypothetical protein yfdQ 28 6.9
TBP_PLAFA (P32086) TATA-box binding protein (TATA-box factor) (T... 28 6.9
PSTB_AQUAE (O67154) Phosphate import ATP-binding protein pstB (E... 28 6.9
SYG_PYRAB (Q9V176) Glycyl-tRNA synthetase (EC 6.1.1.14) (Glycine... 28 9.1
KAB7_SCHPO (Q09815) Probable serine/threonine-protein kinase C16... 28 9.1
>DYHG_CHLRE (Q39575) Dynein gamma chain, flagellar outer arm
Length = 4485
Score = 30.8 bits (68), Expect = 1.1
Identities = 26/81 (32%), Positives = 40/81 (49%), Gaps = 2/81 (2%)
Query: 1 MGEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITV-LKLDT 59
M +GA SGL + V+ + ED + P E E L L G + +TV L+ +
Sbjct: 4195 MPKGAGSGSGLSREDVVDKICEDLLSKAPPLFDKEETKEKLKKLPGGPTLPLTVHLRQEI 4254
Query: 60 TSFDVIL-MNTATLKDLKLAI 79
+++ + T TLK+L LAI
Sbjct: 4255 DRLNIVTRLTTTTLKNLALAI 4275
>HS70_THEAN (P16019) Heat shock 70 kDa protein (HSP 70.1)
Length = 646
Score = 30.4 bits (67), Expect = 1.4
Identities = 22/114 (19%), Positives = 45/114 (39%), Gaps = 8/114 (7%)
Query: 26 LADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMNT----ATLKDLKLAIKK 81
+A P+ + +E ++ + +T + T + + + + +++ +++
Sbjct: 465 IAPAPRG--VPQIEVTFDIDANGILNVTAMDKSTGKSEHVTITNDKGRLSQEEIDRMVEE 522
Query: 82 KVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSST 135
Y E+ R + R NYC S N L++D V Q LG ST
Sbjct: 523 AEKYKEEDEKRRRCVESRHKLENYCYSMKNT--LSEDQVKQKLGADEVDSALST 574
>GLMS_SALTY (Q8ZKX1) Glucosamine--fructose-6-phosphate
aminotransferase [isomerizing] (EC 2.6.1.16)
(Hexosephosphate aminotransferase)
(D-fructose-6-phosphate amidotransferase) (GFAT)
(L-glutamine-D-fructose-6-phosphate amidotransferase)
(Glucosamine-6-ph
Length = 608
Score = 29.6 bits (65), Expect = 2.4
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Query: 2 GEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTS 61
GE A+ +GL+ +K L L + +VP + + + + + G+ + + K TT
Sbjct: 350 GETADTLAGLRLSKEL-GYLGSLAICNVPGSSLVRESDLALMTNAGTEIGVASTKAFTTQ 408
Query: 62 FDVILMNTATLKDLK 76
V+LM A L LK
Sbjct: 409 LTVLLMLVAKLSRLK 423
>GLMS_SALTI (Q8Z2Q2) Glucosamine--fructose-6-phosphate
aminotransferase [isomerizing] (EC 2.6.1.16)
(Hexosephosphate aminotransferase)
(D-fructose-6-phosphate amidotransferase) (GFAT)
(L-glutamine-D-fructose-6-phosphate amidotransferase)
(Glucosamine-6-ph
Length = 608
Score = 29.6 bits (65), Expect = 2.4
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Query: 2 GEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTS 61
GE A+ +GL+ +K L L + +VP + + + + + G+ + + K TT
Sbjct: 350 GETADTLAGLRLSKEL-GYLGSLAICNVPGSSLVRESDLALMTNAGTEIGVASTKAFTTQ 408
Query: 62 FDVILMNTATLKDLK 76
V+LM A L LK
Sbjct: 409 LTVLLMLVAKLSRLK 423
>GLMS_ECOLI (P17169) Glucosamine--fructose-6-phosphate
aminotransferase [isomerizing] (EC 2.6.1.16)
(Hexosephosphate aminotransferase)
(D-fructose-6-phosphate amidotransferase) (GFAT)
(L-glutamine-D-fructose-6-phosphate amidotransferase)
(Glucosamine-6-ph
Length = 608
Score = 29.6 bits (65), Expect = 2.4
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Query: 2 GEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTS 61
GE A+ +GL+ +K L L + +VP + + + + + G+ + + K TT
Sbjct: 350 GETADTLAGLRLSKEL-GYLGSLAICNVPGSSLVRESDLALMTNAGTEIGVASTKAFTTQ 408
Query: 62 FDVILMNTATLKDLK 76
V+LM A L LK
Sbjct: 409 LTVLLMLVAKLSRLK 423
>GLMS_ECOL6 (Q8FBT4) Glucosamine--fructose-6-phosphate
aminotransferase [isomerizing] (EC 2.6.1.16)
(Hexosephosphate aminotransferase)
(D-fructose-6-phosphate amidotransferase) (GFAT)
(L-glutamine-D-fructose-6-phosphate amidotransferase)
(Glucosamine-6-ph
Length = 608
Score = 29.6 bits (65), Expect = 2.4
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Query: 2 GEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTS 61
GE A+ +GL+ +K L L + +VP + + + + + G+ + + K TT
Sbjct: 350 GETADTLAGLRLSKEL-GYLGSLAICNVPGSSLVRESDLALMTNAGTEIGVASTKAFTTQ 408
Query: 62 FDVILMNTATLKDLK 76
V+LM A L LK
Sbjct: 409 LTVLLMLVAKLSRLK 423
>GLMS_ECO57 (Q8XEG2) Glucosamine--fructose-6-phosphate
aminotransferase [isomerizing] (EC 2.6.1.16)
(Hexosephosphate aminotransferase)
(D-fructose-6-phosphate amidotransferase) (GFAT)
(L-glutamine-D-fructose-6-phosphate amidotransferase)
(Glucosamine-6-ph
Length = 608
Score = 29.6 bits (65), Expect = 2.4
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 1/75 (1%)
Query: 2 GEGAEYKSGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTS 61
GE A+ +GL+ +K L L + +VP + + + + + G+ + + K TT
Sbjct: 350 GETADTLAGLRLSKEL-GYLGSLAICNVPGSSLVRESDLALMTNAGTEIGVASTKAFTTQ 408
Query: 62 FDVILMNTATLKDLK 76
V+LM A L LK
Sbjct: 409 LTVLLMLVAKLSRLK 423
>GC34_CAEEL (P92006) Soluble guanylate cyclase gcy-34 (EC 4.6.1.2)
Length = 686
Score = 29.6 bits (65), Expect = 2.4
Identities = 13/44 (29%), Positives = 26/44 (58%)
Query: 50 MRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGH 93
MR+T + L + D+IL+N L D+++ ++ + N + +M H
Sbjct: 373 MRLTAMPLHDATRDLILLNQQRLSDVEVNLQLEANNEQLETMTH 416
>YNV7_YEAST (P40152) Hypothetical 37.2 kDa protein in ALG9-RAP1
intergenic region
Length = 326
Score = 29.3 bits (64), Expect = 3.1
Identities = 13/62 (20%), Positives = 27/62 (42%)
Query: 70 ATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNN 129
++L ++K KK + + ++ W ++W Y F N K+ D L +R
Sbjct: 229 SSLSNMKYVDKKNWSKTSREKENKNYVRWYTLWDKYGDHFSNAKVFYGHDASMGLNLRRQ 288
Query: 130 SQ 131
++
Sbjct: 289 TK 290
>YE74_METJA (Q58869) Hypothetical UPF0129 protein MJ1474
Length = 197
Score = 29.3 bits (64), Expect = 3.1
Identities = 33/131 (25%), Positives = 56/131 (42%), Gaps = 21/131 (16%)
Query: 7 YKSGLKKAKVLKALL----EDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSF 62
Y+SG+ K KVL A +PI+ + T E +E E+ S I LD
Sbjct: 26 YRSGIMKVKVLDASAIIHGYNPIIEEGEHYTTPEVLE-----EIESKKIIVEQALDFGKL 80
Query: 63 DVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDV-L 121
++ N +K ++ +KK + + Q +G L+ + N +L DD +
Sbjct: 81 KIMSPNREYIKKVEEVVKKTGDNLSQQDIG-----------VLALALNLNAILYTDDYGI 129
Query: 122 QNLGVRNNSQV 132
QN+ + N +V
Sbjct: 130 QNVAKKLNIEV 140
>VP5_AHSV4 (Q02168) Outer capsid protein VP5
Length = 505
Score = 28.9 bits (63), Expect = 4.1
Identities = 12/32 (37%), Positives = 23/32 (71%)
Query: 65 ILMNTATLKDLKLAIKKKVNYMEQSSMGHRHI 96
+L +T T KDL +AIK KV+ +++ ++ +H+
Sbjct: 287 MLRDTVTDKDLAMAIKSKVDVIDEMNVETQHV 318
>CAPP_STRA5 (Q8E0H2) Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
(PEPCase) (PEPC)
Length = 931
Score = 28.9 bits (63), Expect = 4.1
Identities = 14/43 (32%), Positives = 25/43 (57%)
Query: 62 FDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWAN 104
F++IL KD+ LAI++ N +E++ M H + +R + N
Sbjct: 848 FNIILNEWQLTKDMILAIEQHDNLLEENPMLHASLDYRLPYFN 890
>CAPP_STRA3 (Q8E647) Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
(PEPCase) (PEPC)
Length = 931
Score = 28.9 bits (63), Expect = 4.1
Identities = 14/43 (32%), Positives = 25/43 (57%)
Query: 62 FDVILMNTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWAN 104
F++IL KD+ LAI++ N +E++ M H + +R + N
Sbjct: 848 FNIILNEWQLTKDMILAIEQHDNLLEENPMLHASLDYRLPYFN 890
>HEM3_PROMI (Q59684) Porphobilinogen deaminase (EC 2.5.1.61) (PBG)
(Hydroxymethylbilane synthase) (HMBS)
(Pre-uroporphyrinogen synthase)
Length = 313
Score = 28.5 bits (62), Expect = 5.3
Identities = 24/83 (28%), Positives = 37/83 (43%), Gaps = 17/83 (20%)
Query: 9 SGLKKAKVLKALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVILMN 68
S L++ LKAL D I+ D+ N +G L KLD +D I++
Sbjct: 128 SSLRRQCQLKALRPDLIIRDLRGN---------VGTRLS--------KLDNGDYDAIILA 170
Query: 69 TATLKDLKLAIKKKVNYMEQSSM 91
A LK LKL + + + + S+
Sbjct: 171 VAGLKRLKLTERIRSSLSAEQSL 193
>GUNA_CALSA (P22534) Endoglucanase A precursor (EC 3.2.1.4)
(Endo-1,4-beta-glucanase A) (Cellulase A)
Length = 1742
Score = 28.5 bits (62), Expect = 5.3
Identities = 15/58 (25%), Positives = 25/58 (42%), Gaps = 1/58 (1%)
Query: 85 YMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQVSSTYLCHCWN 142
Y S + +SW +VW Y + D+ L + +QN + S + HCW+
Sbjct: 226 YYNSWSGFYDELSWAAVWL-YLATNDSTYLTKAESYVQNWPKISGSNIIDYKWAHCWD 282
>YFDQ_ECOLI (P76513) Hypothetical protein yfdQ
Length = 274
Score = 28.1 bits (61), Expect = 6.9
Identities = 22/79 (27%), Positives = 34/79 (42%), Gaps = 1/79 (1%)
Query: 35 LEDVETLIGLELGSAMRITVLKLDTTSFDVILMNTATLKDLKLAIKKKVNYMEQSSMGHR 94
L D T ++ + ++VL L T NTATLK K A + + +
Sbjct: 74 LADEGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGERNSQK 133
Query: 95 HIS-WRSVWANYCLSFDNN 112
++ W WA+Y + FD N
Sbjct: 134 SLAEWIEDWADYLVGFDAN 152
>TBP_PLAFA (P32086) TATA-box binding protein (TATA-box factor) (TATA
binding factor) (TATA sequence-binding protein)
(Transcription initiation factor TFIID TBP subunit)
Length = 228
Score = 28.1 bits (61), Expect = 6.9
Identities = 18/62 (29%), Positives = 34/62 (54%), Gaps = 6/62 (9%)
Query: 83 VNYMEQSSMGHRHISWRSVW-ANYCLSFDNNKLLNDDD-----VLQNLGVRNNSQVSSTY 136
+N++EQ + +I+ +V A+Y +DNN+ DD V +N+ + ++ +SS
Sbjct: 1 MNFLEQDQLFLENINQDNVVSAHYTSEYDNNEKEKSDDLKNKLVHKNISLNIHNIISSAN 60
Query: 137 LC 138
LC
Sbjct: 61 LC 62
>PSTB_AQUAE (O67154) Phosphate import ATP-binding protein pstB (EC
3.6.3.27) (Phosphate-transporting ATPase) (ABC phosphate
transporter)
Length = 257
Score = 28.1 bits (61), Expect = 6.9
Identities = 25/88 (28%), Positives = 41/88 (46%), Gaps = 17/88 (19%)
Query: 10 GLKKAKVL----KALLEDPILADVPKNPTLEDVETLIGLELGSAMRITVLKLDTTSFDVI 65
G+KK +L + L D L D K+ E+ +L G G R+ + + +V+
Sbjct: 119 GIKKKSILDEKVEKALRDAALWDEVKDRLHENAYSLSG---GQQQRLCIARAIAVEPEVL 175
Query: 66 LMN----------TATLKDLKLAIKKKV 83
LM+ TA ++DL + +KKKV
Sbjct: 176 LMDEPTSALDPISTAKIEDLIVELKKKV 203
>SYG_PYRAB (Q9V176) Glycyl-tRNA synthetase (EC 6.1.1.14)
(Glycine--tRNA ligase) (GlyRS)
Length = 571
Score = 27.7 bits (60), Expect = 9.1
Identities = 12/50 (24%), Positives = 26/50 (52%)
Query: 83 VNYMEQSSMGHRHISWRSVWANYCLSFDNNKLLNDDDVLQNLGVRNNSQV 132
V Y E+ S+G R++ + + YC++ DN ++ +++ R +V
Sbjct: 506 VVYDEKDSIGKRYMRYDEIGTPYCVTIDNQTPIDGTVTIRDRDTREQIRV 555
>KAB7_SCHPO (Q09815) Probable serine/threonine-protein kinase
C16C9.07 (EC 2.7.1.37)
Length = 836
Score = 27.7 bits (60), Expect = 9.1
Identities = 22/68 (32%), Positives = 35/68 (51%), Gaps = 3/68 (4%)
Query: 68 NTATLKDLKLAIKKKVNYMEQSSMGHRHISWRSVWANY--CLSFDNNKLLNDDD-VLQNL 124
+ +TL+ L A KV+ Q+S + IS R++ +N+LL D D V++NL
Sbjct: 360 SASTLRRLPRAPGSKVHAERQNSTFNSGISLRALRKEMGNTAPVSSNQLLKDADLVMENL 419
Query: 125 GVRNNSQV 132
RN +V
Sbjct: 420 STRNTEKV 427
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.317 0.133 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,611,096
Number of Sequences: 164201
Number of extensions: 608559
Number of successful extensions: 1637
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 1631
Number of HSP's gapped (non-prelim): 20
length of query: 146
length of database: 59,974,054
effective HSP length: 100
effective length of query: 46
effective length of database: 43,553,954
effective search space: 2003481884
effective search space used: 2003481884
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144765.13