BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144730.5 + phase: 0 /pseudo
(146 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TRA0_MYCSM (P35883) Transposase for insertion sequence element I... 35 0.057
TRA5_LACLA (P35881) Transposase for insertion sequence element I... 31 0.82
CSD_XYLFT (Q87DJ2) Probable cysteine desulfurase (EC 2.8.1.7) 28 5.3
YOH5_YEAST (Q08234) Probable ATP-dependent transporter YOL074C/Y... 28 6.9
TBD_MOUSE (Q9R1K7) Tubulin delta chain (Delta tubulin) 28 6.9
SYY_METTH (O27795) Tyrosyl-tRNA synthetase (EC 6.1.1.1) (Tyrosin... 28 6.9
PRIS_ARCFU (O29516) Probable DNA primase small subunit (EC 2.7.7.-) 28 6.9
DBF2_YEAST (P22204) Cell cycle protein kinase DBF2 (EC 2.7.1.37) 28 6.9
CSD_XYLFA (Q9PDA6) Probable cysteine desulfurase (EC 2.8.1.7) 28 6.9
YCY0_YEAST (P25654) Hypothetical 20.7 kDa protein in FIG2-KIN82 ... 28 9.1
ETC3_STAAM (P23313) Enterotoxin type C-3 precursor (SEC3) 28 9.1
ETC2_STAAU (P34071) Enterotoxin type C-2 precursor (SEC2) 28 9.1
ETC1_STAAU (P01553) Enterotoxin type C-1 precursor (SEC1) 28 9.1
>TRA0_MYCSM (P35883) Transposase for insertion sequence element
IS6120
Length = 323
Score = 35.0 bits (79), Expect = 0.057
Identities = 17/48 (35%), Positives = 23/48 (47%)
Query: 48 MLRKLLTSKMNMPKVIVTDRDMSLMKEVANVFPESYAMNCYFHVQANV 95
+LR M P + + D + K V VFP + C+FH QANV
Sbjct: 113 LLRDCKRRGMTAPVLAIGDGALGFWKAVREVFPATKEQRCWFHKQANV 160
>TRA5_LACLA (P35881) Transposase for insertion sequence element
IS905
Length = 391
Score = 31.2 bits (69), Expect = 0.82
Identities = 16/67 (23%), Positives = 29/67 (42%), Gaps = 1/67 (1%)
Query: 32 GFETHEKEENFVWVLKMLRKLLTSKMNMPKVIVTDRDMSLMKEVANVFPESYAMNCYFHV 91
G+E E N W +L KL + ++VTD L + ++ +P + C H+
Sbjct: 195 GYEIAPNENNASWST-LLDKLQNQGIQQVSLVVTDGFKGLEQIISQAYPLAKQQRCLIHI 253
Query: 92 QANVKQR 98
N+ +
Sbjct: 254 SRNLASK 260
>CSD_XYLFT (Q87DJ2) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 416
Score = 28.5 bits (62), Expect = 5.3
Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Query: 13 PMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLL 53
P+ + GVT T S+ F + THE+ E F+ L +RKLL
Sbjct: 377 PLLQYYGVTAT-CRASLAF-YNTHEEIERFITALLKVRKLL 415
>YOH5_YEAST (Q08234) Probable ATP-dependent transporter
YOL074C/YOL075C
Length = 1294
Score = 28.1 bits (61), Expect = 6.9
Identities = 25/81 (30%), Positives = 38/81 (46%), Gaps = 2/81 (2%)
Query: 25 LTYSVGFGFETHEKEENFVWVLKMLRKLLTSKMNMPKVIVTDRDMSLMKEVANVFPESYA 84
+TY + FG E ++ + + + L +L S ++M V V+ RD S V N+ +
Sbjct: 478 ITYFM-FGLEADARKFFYQFAVVFLCQLSCSGLSMLSVAVS-RDFSKASLVGNMTFTVLS 535
Query: 85 MNCYFHVQANVKQRCVLDCKY 105
M C F V A V V KY
Sbjct: 536 MGCGFFVNAKVMPVYVRWIKY 556
>TBD_MOUSE (Q9R1K7) Tubulin delta chain (Delta tubulin)
Length = 455
Score = 28.1 bits (61), Expect = 6.9
Identities = 11/46 (23%), Positives = 25/46 (53%)
Query: 49 LRKLLTSKMNMPKVIVTDRDMSLMKEVANVFPESYAMNCYFHVQAN 94
+ K+ +MN+ ++ D + L ++ +VF +Y+ + FH + N
Sbjct: 209 VHKICAKRMNIKQISFRDLNQVLAHQLGSVFQPTYSEDSSFHYRRN 254
>SYY_METTH (O27795) Tyrosyl-tRNA synthetase (EC 6.1.1.1)
(Tyrosine--tRNA ligase) (TyrRS)
Length = 319
Score = 28.1 bits (61), Expect = 6.9
Identities = 14/59 (23%), Positives = 29/59 (48%)
Query: 10 YKMPMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLLTSKMNMPKVIVTDRD 68
Y F +G++ + +G F+T + + V+ L ++ LL +K +M ++ RD
Sbjct: 89 YNQKCFLALGLSPERTEFILGSSFQTEPEYTDLVYRLALITTLLRAKRSMAQITRESRD 147
>PRIS_ARCFU (O29516) Probable DNA primase small subunit (EC 2.7.7.-)
Length = 335
Score = 28.1 bits (61), Expect = 6.9
Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 2/51 (3%)
Query: 19 GVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLLTS--KMNMPKVIVTDR 67
G+ + +S G G+ H +E F+ + R+ + ++N PK++V DR
Sbjct: 132 GIRDMKIYFSGGRGYHVHVHDEEFLSLGSAERREIVDYLRLNSPKIVVEDR 182
>DBF2_YEAST (P22204) Cell cycle protein kinase DBF2 (EC 2.7.1.37)
Length = 572
Score = 28.1 bits (61), Expect = 6.9
Identities = 15/42 (35%), Positives = 25/42 (58%), Gaps = 2/42 (4%)
Query: 30 GFG--FETHEKEENFVWVLKMLRKLLTSKMNMPKVIVTDRDM 69
G+G + +K+ V LK+L K L K+N K ++T+RD+
Sbjct: 187 GYGQVYLARKKDTKEVCALKILNKKLLFKLNETKHVLTERDI 228
>CSD_XYLFA (Q9PDA6) Probable cysteine desulfurase (EC 2.8.1.7)
Length = 416
Score = 28.1 bits (61), Expect = 6.9
Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 2/41 (4%)
Query: 13 PMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLL 53
P+ + GVT T S+ F + THE+ E F+ L +RKLL
Sbjct: 377 PLLQYYGVTAT-CRASLAF-YNTHEEIERFIAALLKVRKLL 415
>YCY0_YEAST (P25654) Hypothetical 20.7 kDa protein in FIG2-KIN82
intergenic region
Length = 182
Score = 27.7 bits (60), Expect = 9.1
Identities = 28/112 (25%), Positives = 47/112 (41%), Gaps = 6/112 (5%)
Query: 12 MPMFEVVGVTLTDLTYSVGFGFETHEKEENFVWVLKMLRKLLTSKMNMPKVIVTDRDMSL 71
MP+F V+ TL++ V T+E F + L+ +++ KVI+ +
Sbjct: 1 MPLFLVLKATLSENVTKVSIE-NTNESRAEFAFDLQCTS---CRELHDSKVIINTFEEYA 56
Query: 72 MKEVANVFPESYAMNCYFHVQANVKQRCVLDCKYPLGKKDGKEVKPRDVVNK 123
M A+ S+ M C F + C + +Y + D K K +DV K
Sbjct: 57 MP--ASKGTASFLMKCKFCSKELSVNLCAFEDEYLTDQSDDKWAKIKDVRKK 106
>ETC3_STAAM (P23313) Enterotoxin type C-3 precursor (SEC3)
Length = 266
Score = 27.7 bits (60), Expect = 9.1
Identities = 17/59 (28%), Positives = 31/59 (51%), Gaps = 2/59 (3%)
Query: 74 EVANVFPESYAMNCYFHVQANV-KQRCVLDCKY-PLGKKDGKEVKPRDVVNKIMRAWKS 130
EV +V+ +Y +NCYF + NV K C Y + K +G ++ N ++R +++
Sbjct: 107 EVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLVRVYEN 165
>ETC2_STAAU (P34071) Enterotoxin type C-2 precursor (SEC2)
Length = 266
Score = 27.7 bits (60), Expect = 9.1
Identities = 17/59 (28%), Positives = 31/59 (51%), Gaps = 2/59 (3%)
Query: 74 EVANVFPESYAMNCYFHVQANV-KQRCVLDCKY-PLGKKDGKEVKPRDVVNKIMRAWKS 130
EV +V+ +Y +NCYF + NV K C Y + K +G ++ N ++R +++
Sbjct: 107 EVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLIRVYEN 165
>ETC1_STAAU (P01553) Enterotoxin type C-1 precursor (SEC1)
Length = 266
Score = 27.7 bits (60), Expect = 9.1
Identities = 17/59 (28%), Positives = 31/59 (51%), Gaps = 2/59 (3%)
Query: 74 EVANVFPESYAMNCYFHVQANV-KQRCVLDCKY-PLGKKDGKEVKPRDVVNKIMRAWKS 130
EV +V+ +Y +NCYF + NV K C Y + K +G ++ N ++R +++
Sbjct: 107 EVVDVYGSNYYVNCYFSSKDNVGKVTGGKTCMYGGITKHEGNHFDNGNLQNVLIRVYEN 165
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.320 0.133 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,229,061
Number of Sequences: 164201
Number of extensions: 592839
Number of successful extensions: 1302
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1299
Number of HSP's gapped (non-prelim): 14
length of query: 146
length of database: 59,974,054
effective HSP length: 100
effective length of query: 46
effective length of database: 43,553,954
effective search space: 2003481884
effective search space used: 2003481884
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Medicago: description of AC144730.5