BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144727.12 - phase: 0
(160 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KUP1_LACPL (Q88Z42) Probable potassium transport system protein ... 31 1.0
RPOC_AQUPY (Q9X6Y2) DNA-directed RNA polymerase beta' chain (EC ... 31 1.4
NUON_RHOCA (P50973) NADH-quinone oxidoreductase chain N (EC 1.6.... 31 1.4
NU3M_DINSE (O79553) NADH-ubiquinone oxidoreductase chain 3 (EC 1... 31 1.4
DP3A_CHLTR (O84549) DNA polymerase III alpha subunit (EC 2.7.7.7) 30 1.8
YE01_PROMM (Q7V5Y3) Hypothetical UPF0078 protein PMT1401 30 3.0
DP3A_CHLMU (Q9PJJ7) DNA polymerase III alpha subunit (EC 2.7.7.7) 30 3.0
Y1F5_PSESM (Q87YP0) Hypothetical UPF0059 protein PSPTO3755 29 4.0
TKT2_PASMU (P57958) Transketolase 2 (EC 2.2.1.1) (TK 2) 29 4.0
TKT1_PASMU (P57927) Transketolase 1 (EC 2.2.1.1) (TK 1) 29 4.0
PROB_LISMF (Q720G2) Glutamate 5-kinase (EC 2.7.2.11) (Gamma-glut... 29 4.0
Y456_MYCGE (P47694) Hypothetical protein MG456 29 5.2
TKTP_ALCEU (P21726) Transketolase, plasmid (EC 2.2.1.1) (TK) 29 5.2
YN89_ERWCT (Q6D4K1) Hypothetical UPF0059 protein ECA2389 28 6.8
TR50_PANTR (Q646C3) Taste receptor type 2 member 50 (T2R50) 28 6.8
TR50_PANPA (Q646E4) Taste receptor type 2 member 50 (T2R50) 28 6.8
GP44_MOUSE (Q9Z2J6) Putative G protein-coupled receptor GPR44 (C... 28 8.9
CARB_HELPY (O25577) Carbamoyl-phosphate synthase large chain (EC... 28 8.9
AROA_LEPIN (Q8F6P5) 3-phosphoshikimate 1-carboxyvinyltransferase... 28 8.9
>KUP1_LACPL (Q88Z42) Probable potassium transport system protein
kup1
Length = 660
Score = 31.2 bits (69), Expect = 1.0
Identities = 15/44 (34%), Positives = 24/44 (54%)
Query: 42 IGPGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGIGSLISG 85
+GPG + H +P + + + T V FA +A ++ SLISG
Sbjct: 271 LGPGQAIQGHVNPFFAMLPASLTMAAVIFATLAAIIASQSLISG 314
>RPOC_AQUPY (Q9X6Y2) DNA-directed RNA polymerase beta' chain (EC
2.7.7.6) (RNAP beta' subunit) (Transcriptase beta'
chain) (RNA polymerase beta' subunit)
Length = 1576
Score = 30.8 bits (68), Expect = 1.4
Identities = 19/55 (34%), Positives = 27/55 (48%)
Query: 31 AMNRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGIGSLISG 85
AM I+H EI G + P F+PIY + A G +AG+ G+ + SG
Sbjct: 812 AMFEEIEHSTEIRNGKEYPGTFNPIYMMAVSGARGNRDQIRQLAGMRGLMAKHSG 866
>NUON_RHOCA (P50973) NADH-quinone oxidoreductase chain N (EC
1.6.99.5) (NADH dehydrogenase I, chain N) (NDH-1, chain
N)
Length = 478
Score = 30.8 bits (68), Expect = 1.4
Identities = 27/95 (28%), Positives = 40/95 (41%), Gaps = 14/95 (14%)
Query: 65 GFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQL 124
GFF FA+I +G G + W + A+ + A + V M FG K L
Sbjct: 386 GFFAKFAVIKAAIGAGFV---------WVPVAAVVASVIGAFYYLRIVYFMYFGEKSAPL 436
Query: 125 NIRNSRLKTMEAFLIILTATQL---FYIAAIHGAA 156
+ R L+ AFL++ L +A + GAA
Sbjct: 437 DGRMPALQF--AFLVLAAVAMLGGAINMAGVEGAA 469
>NU3M_DINSE (O79553) NADH-ubiquinone oxidoreductase chain 3 (EC
1.6.5.3)
Length = 114
Score = 30.8 bits (68), Expect = 1.4
Identities = 18/59 (30%), Positives = 29/59 (48%)
Query: 58 PMGNAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMG 116
P+GNA T + F L+A + + L L W+ + PS ++ I LT+L +G
Sbjct: 42 PLGNARTPISIQFFLVAILFILFDLEIVLLLPTPWSMNTNPSNTTILLITMLLTILTLG 100
>DP3A_CHLTR (O84549) DNA polymerase III alpha subunit (EC 2.7.7.7)
Length = 1237
Score = 30.4 bits (67), Expect = 1.8
Identities = 13/46 (28%), Positives = 28/46 (60%), Gaps = 5/46 (10%)
Query: 1 MANEQMKPIATLLLALNFCMYVIVISIGGW-AMNRAIDHGFEIGPG 45
+ E++K ++++++ C Y++++ W +N A DHG +GPG
Sbjct: 370 LVKERLKLESSIIISKGMCDYLLIV----WDIINWAKDHGIPVGPG 411
>YE01_PROMM (Q7V5Y3) Hypothetical UPF0078 protein PMT1401
Length = 204
Score = 29.6 bits (65), Expect = 3.0
Identities = 24/88 (27%), Positives = 40/88 (45%), Gaps = 13/88 (14%)
Query: 29 GWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGIGSLISGLN- 87
GW +A+ G + G I +P+G A G F+T + +V + S+I+ L+
Sbjct: 104 GWKGGKAVATGLGMLLG---------ISWPVGLACFGIFLTVLSFSRIVSLSSIIAALSL 154
Query: 88 ---HIRSWTSESLPSAASVAAIAWALTV 112
I + S P+ +VA A A+ V
Sbjct: 155 PLLMILRFQGNSPPAYLAVAFAAMAMVV 182
>DP3A_CHLMU (Q9PJJ7) DNA polymerase III alpha subunit (EC 2.7.7.7)
Length = 1237
Score = 29.6 bits (65), Expect = 3.0
Identities = 13/46 (28%), Positives = 27/46 (58%), Gaps = 5/46 (10%)
Query: 1 MANEQMKPIATLLLALNFCMYVIVISIGGW-AMNRAIDHGFEIGPG 45
+ E++ +T++++ C Y++++ W +N A DHG +GPG
Sbjct: 370 VVKERLSLESTIIISKGMCDYLLIV----WDIINWAKDHGIPVGPG 411
>Y1F5_PSESM (Q87YP0) Hypothetical UPF0059 protein PSPTO3755
Length = 190
Score = 29.3 bits (64), Expect = 4.0
Identities = 36/150 (24%), Positives = 65/150 (43%), Gaps = 14/150 (9%)
Query: 6 MKPIATLLLALNFCMYVIVISIG-GWAMNRA-IDHGFEIGPGFDLPAHFSPIY-FPMGNA 62
M PI+ L LAL ++G G ++++ G F +P+ + +G
Sbjct: 1 MNPISLLFLALAMSTDAFAAALGKGASLHKPRFIEALRTGLIFGAIETITPVIGWGIGQV 60
Query: 63 ATGF------FVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAA----SVAAIAWALTV 112
A F ++ F L+ V+G+ + +GL H E S + +V A A ++
Sbjct: 61 AARFAESWDHWIAFTLLL-VLGLHMIYNGLKHDHEEEQEKPGSHSFWILAVTAFATSIDA 119
Query: 113 LAMGFGCKEIQLNIRNSRLKTMEAFLIILT 142
LA+G G + +NI + L A +++T
Sbjct: 120 LAVGVGLAFVDVNIMVAALAIGLATTVMVT 149
>TKT2_PASMU (P57958) Transketolase 2 (EC 2.2.1.1) (TK 2)
Length = 668
Score = 29.3 bits (64), Expect = 4.0
Identities = 25/126 (19%), Positives = 52/126 (40%), Gaps = 11/126 (8%)
Query: 23 IVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGIGSL 82
I ++G + + H F PG ++ H + ++ G G +AG +G+G L
Sbjct: 120 ITNAVGFAIAEKTLAHQFN-RPGHEIVDHHTYVFLGDGCLMEGISHEACSLAGTLGLGKL 178
Query: 83 ISGLN--------HIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQLNIRNSRLKTM 134
I+ + H+ W ++ + A W + G ++I ++ ++ +T
Sbjct: 179 IAFYDDNNISIDGHVDGWFTDD--TQKRFEAYGWHVIPAVDGHNPEQILEAVKQAQAETT 236
Query: 135 EAFLII 140
+ LII
Sbjct: 237 KPTLII 242
>TKT1_PASMU (P57927) Transketolase 1 (EC 2.2.1.1) (TK 1)
Length = 668
Score = 29.3 bits (64), Expect = 4.0
Identities = 25/126 (19%), Positives = 52/126 (40%), Gaps = 11/126 (8%)
Query: 23 IVISIGGWAMNRAIDHGFEIGPGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGIGSL 82
I ++G + + H F PG ++ H + ++ G G +AG +G+G L
Sbjct: 120 ITNAVGFAIAEKTLAHQFN-RPGHEIVDHHTYVFLGDGCLMEGISHEACSLAGTLGLGKL 178
Query: 83 ISGLN--------HIRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQLNIRNSRLKTM 134
I+ + H+ W ++ + A W + G ++I ++ ++ +T
Sbjct: 179 IAFYDDNNISIDGHVDGWFTDD--TQKRFEAYGWHVIPAVDGHNPEQILEAVKQAQAETT 236
Query: 135 EAFLII 140
+ LII
Sbjct: 237 KPTLII 242
>PROB_LISMF (Q720G2) Glutamate 5-kinase (EC 2.7.2.11)
(Gamma-glutamyl kinase) (GK)
Length = 276
Score = 29.3 bits (64), Expect = 4.0
Identities = 19/64 (29%), Positives = 31/64 (47%), Gaps = 4/64 (6%)
Query: 77 VGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVL----AMGFGCKEIQLNIRNSRLK 132
VG +L+ G HI T E L S +L A+G GC ++QL++R + +
Sbjct: 15 VGTSTLMYGNGHINLRTIEKLAMVLSDLRNEGKEVILVSSGAIGVGCHKLQLSVRPTSIP 74
Query: 133 TMEA 136
++A
Sbjct: 75 DLQA 78
>Y456_MYCGE (P47694) Hypothetical protein MG456
Length = 334
Score = 28.9 bits (63), Expect = 5.2
Identities = 23/73 (31%), Positives = 34/73 (46%), Gaps = 6/73 (8%)
Query: 66 FFVT------FALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLAMGFGC 119
FF+T F + G+ G+ S+I+ I S+T +P+ AS+ I VL + F
Sbjct: 10 FFLTPNKLNAFLRVIGLCGLFSVIAISFGIYSYTRNEIPNIASLFLIVLGSVVLFLAFVI 69
Query: 120 KEIQLNIRNSRLK 132
L RN LK
Sbjct: 70 HFAALFKRNKLLK 82
Score = 28.5 bits (62), Expect = 6.8
Identities = 21/68 (30%), Positives = 29/68 (41%), Gaps = 3/68 (4%)
Query: 93 TSESLPSAASVAAIAWALTVLAMGFGCKEIQLNIRNSRLKTMEAFLIILTATQLFYIAAI 152
T L + V + +V+A+ FG I RN FLI+L + LF I
Sbjct: 13 TPNKLNAFLRVIGLCGLFSVIAISFG---IYSYTRNEIPNIASLFLIVLGSVVLFLAFVI 69
Query: 153 HGAAAIRR 160
H AA +R
Sbjct: 70 HFAALFKR 77
>TKTP_ALCEU (P21726) Transketolase, plasmid (EC 2.2.1.1) (TK)
Length = 670
Score = 28.9 bits (63), Expect = 5.2
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 10/73 (13%)
Query: 44 PGFDLPAHFSPIYFPMGNAATGFFVTFALIAGVVGIGSLI-----SGLN---HIRSWTSE 95
PGFD+ H + ++ G G +AG +G+G LI +G++ + W ++
Sbjct: 146 PGFDIVDHHTYVFLGDGCLMEGLSHEACSLAGTLGLGKLICLYDDNGISIDGEVAGWFAD 205
Query: 96 SLPSAASVAAIAW 108
P AA W
Sbjct: 206 DTPK--RFAAYGW 216
>YN89_ERWCT (Q6D4K1) Hypothetical UPF0059 protein ECA2389
Length = 187
Score = 28.5 bits (62), Expect = 6.8
Identities = 43/171 (25%), Positives = 68/171 (39%), Gaps = 24/171 (14%)
Query: 6 MKPIATLLLALNFCMYVIVISIGGWAM--NRAIDHGFEIGPGFDLPAHFSPIYFPMGNAA 63
M ATL+LA M SIG A+ N G F + +P+ A
Sbjct: 1 MNMSATLILAFAMSMDAFAASIGKGAVLHNPRFRDAIRTGLIFGVIEAITPLI----GWA 56
Query: 64 TGFF-----------VTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASV----AAIAW 108
GFF V F L+ ++G ++ G E + + + AIA
Sbjct: 57 LGFFASQYILEWDHWVAFTLLL-ILGGRMVVEGFKDSPDCRCEKVKNHSLALLVCTAIAT 115
Query: 109 ALTVLAMGFGCKEIQLNIRNSRLKTMEAFLIILTATQLF--YIAAIHGAAA 157
+L +A+G G +Q+NI ++ + A +I++T + YI I G A
Sbjct: 116 SLDAMAIGVGLAFLQVNIFHTAMVIGCATMIMVTLGMMIGRYIGPILGKKA 166
>TR50_PANTR (Q646C3) Taste receptor type 2 member 50 (T2R50)
Length = 299
Score = 28.5 bits (62), Expect = 6.8
Identities = 24/98 (24%), Positives = 40/98 (40%), Gaps = 2/98 (2%)
Query: 55 IYFPMGNAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLA 114
+ F +GN A GF I V IS + I + + S + W LTVL
Sbjct: 15 VLFVLGNFANGFIALVNFIDWVKR--KKISSADQILTALAVSRIGLLWTLLLNWYLTVLN 72
Query: 115 MGFGCKEIQLNIRNSRLKTMEAFLIILTATQLFYIAAI 152
F E+++ N+ + T + + + +FY+ I
Sbjct: 73 PAFYSVELRITSYNAWVVTNHFSMWLAASLSIFYLLKI 110
>TR50_PANPA (Q646E4) Taste receptor type 2 member 50 (T2R50)
Length = 299
Score = 28.5 bits (62), Expect = 6.8
Identities = 24/98 (24%), Positives = 40/98 (40%), Gaps = 2/98 (2%)
Query: 55 IYFPMGNAATGFFVTFALIAGVVGIGSLISGLNHIRSWTSESLPSAASVAAIAWALTVLA 114
+ F +GN A GF I V IS + I + + S + W LTVL
Sbjct: 15 VLFVLGNFANGFIALVNFIDWVKR--KKISSADQILTALAVSRIGLLWTLLLNWYLTVLN 72
Query: 115 MGFGCKEIQLNIRNSRLKTMEAFLIILTATQLFYIAAI 152
F E+++ N+ + T + + + +FY+ I
Sbjct: 73 PAFYSVELRITSYNAWVVTNHFSMWLAASLSIFYLLKI 110
>GP44_MOUSE (Q9Z2J6) Putative G protein-coupled receptor GPR44
(Chemoattractant receptor-homologous molecule expressed
on Th2 cells)
Length = 382
Score = 28.1 bits (61), Expect = 8.9
Identities = 21/76 (27%), Positives = 31/76 (40%), Gaps = 4/76 (5%)
Query: 68 VTFALIAGVVGIGSLISGLNH----IRSWTSESLPSAASVAAIAWALTVLAMGFGCKEIQ 123
V F L G I SL+ H +R S LP S+A + L F C ++
Sbjct: 252 VAFVLCWGPYHIFSLLEARAHSVTTLRQLASRGLPFVTSLAFFNSVVNPLLYVFTCPDML 311
Query: 124 LNIRNSRLKTMEAFLI 139
+R S +E+ L+
Sbjct: 312 YKLRRSLRAVLESVLV 327
>CARB_HELPY (O25577) Carbamoyl-phosphate synthase large chain (EC
6.3.5.5) (Carbamoyl-phosphate synthetase ammonia chain)
Length = 1085
Score = 28.1 bits (61), Expect = 8.9
Identities = 16/36 (44%), Positives = 22/36 (60%)
Query: 94 SESLPSAASVAAIAWALTVLAMGFGCKEIQLNIRNS 129
S +L S A+ IA T+LA+GF EIQ +I N+
Sbjct: 305 SSALASKATGFPIAKVATMLAVGFSLDEIQNDITNT 340
>AROA_LEPIN (Q8F6P5) 3-phosphoshikimate 1-carboxyvinyltransferase
(EC 2.5.1.19) (5-enolpyruvylshikimate-3-phosphate
synthase) (EPSP synthase) (EPSPS)
Length = 440
Score = 28.1 bits (61), Expect = 8.9
Identities = 12/31 (38%), Positives = 20/31 (63%)
Query: 60 GNAATGFFVTFALIAGVVGIGSLISGLNHIR 90
GNA TG ++ LI G+ GI + ++G N ++
Sbjct: 89 GNAGTGIRLSAGLICGLPGINATLTGDNSLK 119
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.328 0.140 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,949,356
Number of Sequences: 164201
Number of extensions: 651093
Number of successful extensions: 2167
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 2160
Number of HSP's gapped (non-prelim): 20
length of query: 160
length of database: 59,974,054
effective HSP length: 101
effective length of query: 59
effective length of database: 43,389,753
effective search space: 2559995427
effective search space used: 2559995427
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 61 (28.1 bits)
Medicago: description of AC144727.12