BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144722.14 - phase: 0
(220 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contain... 82 1e-15
M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810... 74 2e-13
POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from tran... 65 1e-10
YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein 62 1e-09
YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B) 36 0.081
YMD9_YEAST (Q03434) Transposon Ty1 protein B 35 0.18
YJZ7_YEAST (P47098) Transposon Ty1 protein B 35 0.18
YMU0_YEAST (Q04670) Transposon Ty1 protein B 34 0.31
YME4_YEAST (Q04711) Transposon Ty1 protein B 34 0.31
RPC2_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain (EC... 34 0.31
YMT5_YEAST (Q04214) Transposon Ty1 protein B 33 0.40
YJZ9_YEAST (P47100) Transposon Ty1 protein B 33 0.40
MDM4_MOUSE (O35618) Mdm4 protein (p53-binding protein Mdm4) (Mdm... 32 1.5
M240_ARATH (P93290) Hypothetical mitochondrial protein AtMg00240... 32 1.5
RPC2_SOYBN (Q8HVY3) DNA-directed RNA polymerase beta'' chain (EC... 31 2.0
SC13_ARATH (Q9FQ19) Sister chromatid cohesion 1 protein 3 (SCC1 ... 31 2.6
YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein 30 3.4
YA49_MYCPN (P75065) Hypothetical protein MPN049 (D09_orf632) 30 4.4
RPC2_SPIOL (P11704) DNA-directed RNA polymerase beta'' chain (EC... 30 4.4
RPC2_ATRBE (Q8S8Y1) DNA-directed RNA polymerase beta'' chain (EC... 30 4.4
>COPI_DROME (P04146) Copia protein (Gag-int-pol protein) [Contains:
Copia VLP protein; Copia protease (EC 3.4.23.-)]
Length = 1409
Score = 81.6 bits (200), Expect = 1e-15
Identities = 45/118 (38%), Positives = 70/118 (59%), Gaps = 1/118 (0%)
Query: 1 MMQVFEMSDLGEMSYFLVIEVEQKNGDIFICQRKYANEMLKKFNMENRKSMSTPMCPKER 60
+M+ F M+DL E+ +F+ I +E + I++ Q Y ++L KFNMEN ++STP+ K
Sbjct: 1108 LMEKFRMTDLNEIKHFIGIRIEMQEDKIYLSQSAYVKKILSKFNMENCNAVSTPLPSKIN 1167
Query: 61 LCKDVEEQVDETLYQSLIGCLMY-LTTTRSDILYDVNVLSRFMNCAKESLFKGAKRVL 117
++ T +SLIGCLMY + TR D+ VN+LSR+ + L++ KRVL
Sbjct: 1168 YELLNSDEDCNTPCRSLIGCLMYIMLCTRPDLTTAVNILSRYSSKNNSELWQNLKRVL 1225
Score = 79.7 bits (195), Expect = 5e-15
Identities = 39/99 (39%), Positives = 60/99 (60%)
Query: 121 LAATTAVNQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFL 180
+A AV +ALWL+ +LT ++++ ++ DN I+I+ N H + KH IK F
Sbjct: 1299 MALFEAVREALWLKFLLTSINIKLENPIKIYEDNQGCISIANNPSCHKRAKHIDIKYHFA 1358
Query: 181 RDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELLREK 219
R+ ++ +CL+Y TE+QL+ IFTK LP RF LR+K
Sbjct: 1359 REQVQNNVICLEYIPTENQLADIFTKPLPAARFVELRDK 1397
>M810_ARATH (P92519) Hypothetical mitochondrial protein AtMg00810
(ORF240b)
Length = 240
Score = 74.3 bits (181), Expect = 2e-13
Identities = 43/120 (35%), Positives = 65/120 (53%)
Query: 1 MMQVFEMSDLGEMSYFLVIEVEQKNGDIFICQRKYANEMLKKFNMENRKSMSTPMCPKER 60
+ F M DLG + YFL I+++ +F+ Q KYA ++L M + K MSTP+ K
Sbjct: 27 LSSTFSMKDLGPVHYFLGIQIKTHPSGLFLSQTKYAEQILNNAGMLDCKPMSTPLPLKLN 86
Query: 61 LCKDVEEQVDETLYQSLIGCLMYLTTTRSDILYDVNVLSRFMNCAKESLFKGAKRVLSRV 120
+ D + ++S++G L YLT TR DI Y VN++ + M+ + F KRVL V
Sbjct: 87 SSVSTAKYPDPSDFRSIVGALQYLTLTRPDISYAVNIVCQRMHEPTLADFDLLKRVLRYV 146
>POLX_TOBAC (P10978) Retrovirus-related Pol polyprotein from
transposon TNT 1-94 [Contains: Protease (EC 3.4.23.-);
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1328
Score = 65.1 bits (157), Expect = 1e-10
Identities = 45/131 (34%), Positives = 73/131 (55%), Gaps = 11/131 (8%)
Query: 5 FEMSDLGEMSYFLVIEV--EQKNGDIFICQRKYANEMLKKFNMENRKSMSTPMCPKERLC 62
F+M DLG L +++ E+ + +++ Q KY +L++FNM+N K +STP+ +L
Sbjct: 1033 FDMKDLGPAQQILGMKIVRERTSRKLWLSQEKYIERVLERFNMKNAKPVSTPLAGHLKLS 1092
Query: 63 K-----DVEEQ--VDETLYQSLIGCLMY-LTTTRSDILYDVNVLSRFMNCAKESLFKGAK 114
K VEE+ + + Y S +G LMY + TR DI + V V+SRF+ + ++ K
Sbjct: 1093 KKMCPTTVEEKGNMAKVPYSSAVGSLMYAMVCTRPDIAHAVGVVSRFLENPGKEHWEAVK 1152
Query: 115 RVLSRVLAATT 125
+L R L TT
Sbjct: 1153 WIL-RYLRGTT 1162
Score = 63.5 bits (153), Expect = 4e-10
Identities = 30/98 (30%), Positives = 57/98 (57%), Gaps = 1/98 (1%)
Query: 121 LAATTAVNQALWLRKVLTDLHLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFL 180
+AAT + +WL++ L +L L Q+E V D+ + I +SKN ++H +TKH ++ ++
Sbjct: 1225 IAATETGKEMIWLKRFLQELGLHQKEYV-VYCDSQSAIDLSKNSMYHARTKHIDVRYHWI 1283
Query: 181 RDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELLRE 218
R++ D + + T + + + TK +P +FEL +E
Sbjct: 1284 REMVDDESLKVLKISTNENPADMLTKVVPRNKFELCKE 1321
>YCH4_YEAST (P25600) Transposon Ty5-1 34.5 kDa hypothetical protein
Length = 308
Score = 62.0 bits (149), Expect = 1e-09
Identities = 38/120 (31%), Positives = 65/120 (53%), Gaps = 3/120 (2%)
Query: 1 MMQVFEMSDLGEMSYFLVIEVEQK-NGDIFICQRKYANEMLKKFNMENRKSMSTPMCPKE 59
+ +++ M DLG++ FL + + Q NGDI + + Y + + + K TP+C +
Sbjct: 108 LTKLYSMKDLGKVDKFLGLNIHQSTNGDITLSLQDYIAKAASESEINTFKLTQTPLCNSK 167
Query: 60 RLCKDVEEQV-DETLYQSLIGCLMYLTTT-RSDILYDVNVLSRFMNCAKESLFKGAKRVL 117
L + + D T YQS++G L++ T R DI Y V++LSRF+ + + A+RVL
Sbjct: 168 PLFETTSPHLKDITPYQSIVGQLLFCANTGRPDISYPVSLLSRFLREPRAIHLESARRVL 227
>YCB9_YEAST (P25384) Transposon Ty2 protein B (Ty1-17 protein B)
Length = 1770
Score = 35.8 bits (81), Expect = 0.081
Identities = 30/114 (26%), Positives = 55/114 (47%), Gaps = 4/114 (3%)
Query: 111 KGAKRVLSRVLAATTAVNQALWLRKVLTDLHLEQRETTEV---MVDNHATIAISKN-HVF 166
K + S A AV++A+ L L+ L E + + + D+ +TI+I K+ +
Sbjct: 1655 KASLTCTSTTEAEIHAVSEAIPLLNNLSHLVQELNKKPIIKGLLTDSRSTISIIKSTNEE 1714
Query: 167 HGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELLREKF 220
+ + F K LRD ++ + Y +T+ ++ + TK LP F+LL K+
Sbjct: 1715 KFRNRFFGTKAMRLRDEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNKW 1768
>YMD9_YEAST (Q03434) Transposon Ty1 protein B
Length = 1328
Score = 34.7 bits (78), Expect = 0.18
Identities = 20/72 (27%), Positives = 39/72 (53%), Gaps = 1/72 (1%)
Query: 150 VMVDNHATIAISKN-HVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKAL 208
++ D+ +TI+I K+ + + + F K LRD ++ + Y +T+ ++ + TK L
Sbjct: 1255 LLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYYIETKKNIADVMTKPL 1314
Query: 209 PTGRFELLREKF 220
P F+LL K+
Sbjct: 1315 PIKTFKLLTNKW 1326
>YJZ7_YEAST (P47098) Transposon Ty1 protein B
Length = 1755
Score = 34.7 bits (78), Expect = 0.18
Identities = 20/72 (27%), Positives = 39/72 (53%), Gaps = 1/72 (1%)
Query: 150 VMVDNHATIAISKN-HVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKAL 208
++ D+ +TI+I K+ + + + F K LRD ++ + Y +T+ ++ + TK L
Sbjct: 1682 LLTDSRSTISIIKSTNEEKFRNRFFGTKAMRLRDEVSGNNLYVYYIETKKNIADVMTKPL 1741
Query: 209 PTGRFELLREKF 220
P F+LL K+
Sbjct: 1742 PIKTFKLLTNKW 1753
>YMU0_YEAST (Q04670) Transposon Ty1 protein B
Length = 1328
Score = 33.9 bits (76), Expect = 0.31
Identities = 29/114 (25%), Positives = 54/114 (46%), Gaps = 4/114 (3%)
Query: 111 KGAKRVLSRVLAATTAVNQALWLRKVLTDLHLEQRE---TTEVMVDNHATIAIS-KNHVF 166
K + S A A+++++ L L+ L E + T ++ D+ +TI+I N+
Sbjct: 1213 KASLTCTSTTEAEIHAISESVPLLNNLSHLVQELNKKPITKGLLTDSKSTISIIISNNEE 1272
Query: 167 HGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELLREKF 220
+ + F K LRD + + Y +T+ ++ + TK LP F+LL K+
Sbjct: 1273 KFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADVMTKPLPIKTFKLLTNKW 1326
>YME4_YEAST (Q04711) Transposon Ty1 protein B
Length = 1328
Score = 33.9 bits (76), Expect = 0.31
Identities = 29/114 (25%), Positives = 54/114 (46%), Gaps = 4/114 (3%)
Query: 111 KGAKRVLSRVLAATTAVNQALWLRKVLTDLHLEQRE---TTEVMVDNHATIAIS-KNHVF 166
K + S A A+++++ L L+ L E + T ++ D+ +TI+I N+
Sbjct: 1213 KASLTCTSTTEAEIHAISESVPLLNNLSHLVQELNKKPITKGLLTDSKSTISIIISNNEE 1272
Query: 167 HGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFTKALPTGRFELLREKF 220
+ + F K LRD + + Y +T+ ++ + TK LP F+LL K+
Sbjct: 1273 KFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADVMTKPLPIKTFKLLTNKW 1326
>RPC2_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1332
Score = 33.9 bits (76), Expect = 0.31
Identities = 31/132 (23%), Positives = 62/132 (46%), Gaps = 10/132 (7%)
Query: 82 MYLTTTRSDILYDVNVLSRFMNCAKESLFKGAKRVLSRVLAATTAVNQALWLRK-VLTDL 140
+Y+T +DI+++V + + + + ++++++ + A T +N +RK + +D
Sbjct: 385 LYVTIESNDIMHNVIIPPKSFLLVQNDQYVKSEQIIAEIRAGTYTLNLKEKVRKHIFSDS 444
Query: 141 HLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQL 200
E +T + + + A S H+ KT H I + K V L K +DQ+
Sbjct: 445 EGEMHWSTNIY--HVSEFAYSNVHIL-PKTSHLWI---LSGNSHKSDTVSLSLLKDQDQM 498
Query: 201 SYIFTKALPTGR 212
S T +LPT +
Sbjct: 499 S---THSLPTAK 507
>YMT5_YEAST (Q04214) Transposon Ty1 protein B
Length = 1328
Score = 33.5 bits (75), Expect = 0.40
Identities = 21/75 (28%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Query: 147 TTEVMVDNHATIAIS-KNHVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFT 205
T ++ D+ +TI+I N+ + + F K LRD + + Y +T+ ++ + T
Sbjct: 1252 TKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADVMT 1311
Query: 206 KALPTGRFELLREKF 220
K LP F+LL K+
Sbjct: 1312 KPLPIKTFKLLTNKW 1326
>YJZ9_YEAST (P47100) Transposon Ty1 protein B
Length = 1755
Score = 33.5 bits (75), Expect = 0.40
Identities = 21/75 (28%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Query: 147 TTEVMVDNHATIAIS-KNHVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQLSYIFT 205
T ++ D+ +TI+I N+ + + F K LRD + + Y +T+ ++ + T
Sbjct: 1679 TKGLLTDSKSTISIIISNNEEKFRNRFFGTKAMRLRDEVSGNHLHVCYIETKKNIADVMT 1738
Query: 206 KALPTGRFELLREKF 220
K LP F+LL K+
Sbjct: 1739 KPLPIKTFKLLTNKW 1753
>MDM4_MOUSE (O35618) Mdm4 protein (p53-binding protein Mdm4)
(Mdm2-like p53-binding protein) (Mdmx protein) (Double
minute 4 protein)
Length = 489
Score = 31.6 bits (70), Expect = 1.5
Identities = 22/71 (30%), Positives = 30/71 (41%), Gaps = 9/71 (12%)
Query: 138 TDLHLEQRETTEVMVDNHATIAISK--------NHVFHGKTKHFSIKLFFLRDVQKDGDV 189
TD+ EQ+ TE M D + ++ HGKT H + R ++K G
Sbjct: 413 TDIFSEQKAETESMEDFQNVLKPCSLCEKRPRDGNIIHGKTSHLTTCFHCARRLKKSGAS 472
Query: 190 CLKYCKTEDQL 200
C CK E QL
Sbjct: 473 C-PVCKKEIQL 482
>M240_ARATH (P93290) Hypothetical mitochondrial protein AtMg00240
(ORF111a)
Length = 111
Score = 31.6 bits (70), Expect = 1.5
Identities = 15/39 (38%), Positives = 24/39 (61%)
Query: 82 MYLTTTRSDILYDVNVLSRFMNCAKESLFKGAKRVLSRV 120
MYLT TR D+ + VN LS+F + ++ + + +VL V
Sbjct: 1 MYLTITRPDLTFAVNRLSQFSSASRTAQMQAVYKVLHYV 39
>RPC2_SOYBN (Q8HVY3) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1384
Score = 31.2 bits (69), Expect = 2.0
Identities = 29/121 (23%), Positives = 54/121 (43%), Gaps = 7/121 (5%)
Query: 82 MYLTTTRSDILYDVNVLSRFMNCAKESLFKGAKRVLSRVLAATTAVNQALWLRK-VLTDL 140
+Y+T DI+++V + + + + + +++V++ + A T N +RK V +DL
Sbjct: 389 LYVTIESGDIIHNVTIPPKSFLLVQNNQYVKSEQVIAEIRAGTYTFNLKEKVRKHVYSDL 448
Query: 141 HLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQL 200
E +T+V HA+ N KT H I + G V K +DQ+
Sbjct: 449 EGEMHWSTDVY---HASEFRYSNVHILPKTSHLWI---LSGKSYRSGSVSFSIRKDQDQM 502
Query: 201 S 201
+
Sbjct: 503 N 503
>SC13_ARATH (Q9FQ19) Sister chromatid cohesion 1 protein 3 (SCC1
homolog 3) (AtRAD21-2)
Length = 693
Score = 30.8 bits (68), Expect = 2.6
Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 12/64 (18%)
Query: 141 HLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFF---------LRDVQKD---GD 188
+L+QR ++ +H + +S + + GKT+ + ++FF L D+Q+D GD
Sbjct: 619 YLKQRSSSSPTTSSHPSGDLSLSEILAGKTRKLAARMFFETLVLKSRGLIDMQQDRPYGD 678
Query: 189 VCLK 192
+ LK
Sbjct: 679 IALK 682
>YJL3_YEAST (P47024) Transposon Ty4 207.7 kDa hypothetical protein
Length = 1803
Score = 30.4 bits (67), Expect = 3.4
Identities = 29/96 (30%), Positives = 48/96 (49%), Gaps = 11/96 (11%)
Query: 26 GDIFICQRKYANEMLKKFNMENRK-------SMST-PMCPKERLCKDVEEQVDETL--YQ 75
G I + + + N M KK+N E +K MST + PK+ + + EE+ + + Q
Sbjct: 1515 GTIDLTLKSFINRMDKKYNEELKKIRKSSIPHMSTYKIDPKKDVLQMSEEEFRQGVLKLQ 1574
Query: 76 SLIGCLMYLT-TTRSDILYDVNVLSRFMNCAKESLF 110
L+G L Y+ R DI + V ++R +N E +F
Sbjct: 1575 QLLGELNYVRHKCRYDIEFAVKKVARLVNYPHERVF 1610
>YA49_MYCPN (P75065) Hypothetical protein MPN049 (D09_orf632)
Length = 632
Score = 30.0 bits (66), Expect = 4.4
Identities = 13/40 (32%), Positives = 24/40 (59%)
Query: 32 QRKYANEMLKKFNMENRKSMSTPMCPKERLCKDVEEQVDE 71
Q+++ E+L F E K+++ +ER+ K++EEQ E
Sbjct: 311 QQQFETEVLALFKAEREKALAEYKAEQERIAKELEEQRKE 350
>RPC2_SPIOL (P11704) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1361
Score = 30.0 bits (66), Expect = 4.4
Identities = 17/70 (24%), Positives = 38/70 (54%), Gaps = 1/70 (1%)
Query: 82 MYLTTTRSDILYDVNVLSRFMNCAKESLFKGAKRVLSRVLAATTAVNQALWLRK-VLTDL 140
+Y+T DIL++VN+ + + + +++V++ + A T+ +N +RK + +D
Sbjct: 389 LYVTIESDDILHNVNIPPKSFLLVQNDQYVESEQVIAEIRAGTSTLNFKERVRKHIYSDS 448
Query: 141 HLEQRETTEV 150
E +T+V
Sbjct: 449 EGEMHWSTDV 458
>RPC2_ATRBE (Q8S8Y1) DNA-directed RNA polymerase beta'' chain (EC
2.7.7.6) (PEP) (Plastid-encoded RNA polymerase beta''
subunit) (RNA polymerase beta'' subunit)
Length = 1389
Score = 30.0 bits (66), Expect = 4.4
Identities = 30/121 (24%), Positives = 55/121 (44%), Gaps = 7/121 (5%)
Query: 82 MYLTTTRSDILYDVNVLSRFMNCAKESLFKGAKRVLSRVLAATTAVNQALWLRK-VLTDL 140
+Y+T DIL++VN+ + + + + +++V++ + A + +N +RK + +D
Sbjct: 389 LYVTIESEDILHNVNIPPKSLLLVQNDQYVESEQVIAEIRAGISTLNFKEKVRKHIYSDS 448
Query: 141 HLEQRETTEVMVDNHATIAISKNHVFHGKTKHFSIKLFFLRDVQKDGDVCLKYCKTEDQL 200
E +T+V HA N KT H I L K V L K +DQ+
Sbjct: 449 DGEMHWSTDVY---HAPEFTYGNVHLLPKTSHLWI---LLGGPCKSSLVYLSIHKDQDQM 502
Query: 201 S 201
+
Sbjct: 503 N 503
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.324 0.136 0.393
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,008,406
Number of Sequences: 164201
Number of extensions: 849589
Number of successful extensions: 2582
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 2558
Number of HSP's gapped (non-prelim): 31
length of query: 220
length of database: 59,974,054
effective HSP length: 106
effective length of query: 114
effective length of database: 42,568,748
effective search space: 4852837272
effective search space used: 4852837272
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 63 (28.9 bits)
Medicago: description of AC144722.14