BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144618.2 + phase: 0
(106 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CH60_STAEP (P48218) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 32 0.27
CH60_CLOBO (Q8KJ24) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 32 0.35
FLT2_BRARE (Q98TZ8) Flotillin-2a (Reggie-1a) (REG-1) 31 0.46
FLT2_RAT (Q9Z2S9) Flotillin-2 (Reggie-1) (REG-1) 31 0.60
FLT2_MOUSE (Q60634) Flotillin-2 (Epidermal surface antigen) (ESA) 31 0.60
FLT2_HUMAN (Q14254) Flotillin-2 (Epidermal surface antigen) (ESA) 31 0.60
CH60_CLOPE (P26821) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 31 0.60
CH60_STRPN (Q97NV4) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 0.78
CH60_CLOTM (P48212) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 0.78
UDPE_GVLO (Q98166) Ecdysteroid UDP-glucosyltransferase precursor... 30 1.0
CH60_THETN (Q8R5T7) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 1.0
CH60_CLOTE (Q891G4) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 1.0
CH60_STAAW (P63767) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 1.3
CH60_STAAU (Q08854) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 1.3
CH60_STAAN (P99083) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 1.3
CH60_STAAM (P63766) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 1.3
CH60_CLOAB (P30717) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 30 1.3
SYL_PYRHO (O58698) Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine-... 29 1.7
RUBC_CHLRE (Q42695) RuBisCO subunit binding-protein beta-2 subun... 29 1.7
PEX3_PICAN (Q01497) Peroxisomal biogenesis factor 3 (Peroxin-3) ... 29 1.7
>CH60_STAEP (P48218) 60 kDa chaperonin (Protein Cpn60) (groEL
protein) (Heat shock protein 60)
Length = 538
Score = 32.0 bits (71), Expect = 0.27
Identities = 19/51 (37%), Positives = 25/51 (48%)
Query: 27 GDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAE 77
G K QIE T E D KL L K G ++ + A +TE K+ K + E
Sbjct: 344 GQIKAQIEETDSEFDKEKLQERLAKLAGGVAVIKVGAASETELKERKLRIE 394
>CH60_CLOBO (Q8KJ24) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 543
Score = 31.6 bits (70), Expect = 0.35
Identities = 21/55 (38%), Positives = 27/55 (48%)
Query: 27 GDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVA 81
G K QIE T E D KL L K G +V + A +TE K+ K + E +A
Sbjct: 345 GQIKRQIEETTSEFDKEKLQERLAKIAGGVAVVKVGAATETELKERKLRIEDALA 399
>FLT2_BRARE (Q98TZ8) Flotillin-2a (Reggie-1a) (REG-1)
Length = 428
Score = 31.2 bits (69), Expect = 0.46
Identities = 16/28 (57%), Positives = 20/28 (71%)
Query: 51 KKFGYADLVSIEAVGKTEEKKDKEKAEA 78
K+ G A+ SIEAVGK E +K + KAEA
Sbjct: 320 KRIGEAEAGSIEAVGKAEAEKMRLKAEA 347
>FLT2_RAT (Q9Z2S9) Flotillin-2 (Reggie-1) (REG-1)
Length = 428
Score = 30.8 bits (68), Expect = 0.60
Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
Query: 37 GEEIDSVKLASLLR---KKFGYADLVSIEAVGKTEEKKDKEKAEA 78
GE++ V LA +K G A+ IEA+GK E ++ K KAEA
Sbjct: 303 GEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEA 347
>FLT2_MOUSE (Q60634) Flotillin-2 (Epidermal surface antigen) (ESA)
Length = 379
Score = 30.8 bits (68), Expect = 0.60
Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
Query: 37 GEEIDSVKLASLLR---KKFGYADLVSIEAVGKTEEKKDKEKAEA 78
GE++ V LA +K G A+ IEA+GK E ++ K KAEA
Sbjct: 254 GEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEA 298
>FLT2_HUMAN (Q14254) Flotillin-2 (Epidermal surface antigen) (ESA)
Length = 379
Score = 30.8 bits (68), Expect = 0.60
Identities = 19/45 (42%), Positives = 26/45 (57%), Gaps = 3/45 (6%)
Query: 37 GEEIDSVKLASLLR---KKFGYADLVSIEAVGKTEEKKDKEKAEA 78
GE++ V LA +K G A+ IEA+GK E ++ K KAEA
Sbjct: 254 GEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEA 298
>CH60_CLOPE (P26821) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 539
Score = 30.8 bits (68), Expect = 0.60
Identities = 19/52 (36%), Positives = 26/52 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVA 81
K Q+E T E D KL L K G +V + A +TE K+ K + E +A
Sbjct: 348 KLQLEATTSEFDKEKLQERLAKLAGGVAVVKVGAATETELKESKLRIEDALA 399
>CH60_STRPN (Q97NV4) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 540
Score = 30.4 bits (67), Expect = 0.78
Identities = 18/48 (37%), Positives = 24/48 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAE 77
K QIE T E D KL L K G ++ + A +TE K+ K + E
Sbjct: 348 KSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKLRIE 395
>CH60_CLOTM (P48212) 60 kDa chaperonin (Protein Cpn60) (groEL
protein) (HSP-60)
Length = 540
Score = 30.4 bits (67), Expect = 0.78
Identities = 18/52 (34%), Positives = 26/52 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVA 81
K QIE T + D KL L K G ++ + A +TE K+ K + E +A
Sbjct: 347 KSQIEETTSDFDREKLQERLAKLAGGVAVIQVGAATETEMKEKKLRIEDALA 398
>UDPE_GVLO (Q98166) Ecdysteroid UDP-glucosyltransferase precursor
(EC 2.4.1.-)
Length = 460
Score = 30.0 bits (66), Expect = 1.0
Identities = 20/61 (32%), Positives = 26/61 (41%)
Query: 3 NQKLRNKAMAIAVGVSGVEGTTIKGDNKDQIEVTGEEIDSVKLASLLRKKFGYADLVSIE 62
N ++N M GV E T K + I + EI S + LLR K DL+ E
Sbjct: 75 NNLIKNSTMIKKRGVVADETTVTKENYMGLINLVAHEIKSPNVTRLLRNKGNKFDLIVCE 134
Query: 63 A 63
A
Sbjct: 135 A 135
>CH60_THETN (Q8R5T7) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 540
Score = 30.0 bits (66), Expect = 1.0
Identities = 18/52 (34%), Positives = 26/52 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVA 81
K QIE T + D KL L K G ++ + A +TE K+ K + E +A
Sbjct: 348 KAQIEETTSDYDREKLQERLAKLAGGVAVIQVGAATETELKEKKHRIEDALA 399
>CH60_CLOTE (Q891G4) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 543
Score = 30.0 bits (66), Expect = 1.0
Identities = 19/52 (36%), Positives = 26/52 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVA 81
K QIE T E D KL L K G ++ + A +TE K+ K + E +A
Sbjct: 350 KAQIEETTSEFDREKLQERLAKLAGGVAVIKVGAATETELKERKLRIEDALA 401
>CH60_STAAW (P63767) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 538
Score = 29.6 bits (65), Expect = 1.3
Identities = 17/48 (35%), Positives = 24/48 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAE 77
K QIE T + D KL L K G ++ + A +TE K+ K + E
Sbjct: 348 KSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIE 395
>CH60_STAAU (Q08854) 60 kDa chaperonin (Protein Cpn60) (groEL
protein) (Heat shock protein 60)
Length = 538
Score = 29.6 bits (65), Expect = 1.3
Identities = 17/48 (35%), Positives = 24/48 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAE 77
K QIE T + D KL L K G ++ + A +TE K+ K + E
Sbjct: 348 KSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIE 395
>CH60_STAAN (P99083) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 538
Score = 29.6 bits (65), Expect = 1.3
Identities = 17/48 (35%), Positives = 24/48 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAE 77
K QIE T + D KL L K G ++ + A +TE K+ K + E
Sbjct: 348 KSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIE 395
>CH60_STAAM (P63766) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 538
Score = 29.6 bits (65), Expect = 1.3
Identities = 17/48 (35%), Positives = 24/48 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAE 77
K QIE T + D KL L K G ++ + A +TE K+ K + E
Sbjct: 348 KSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKLRIE 395
>CH60_CLOAB (P30717) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 543
Score = 29.6 bits (65), Expect = 1.3
Identities = 18/52 (34%), Positives = 26/52 (49%)
Query: 30 KDQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVA 81
+ QIE T + D KL L K G +V + A +TE K+ K + E +A
Sbjct: 348 RGQIEETTSDFDREKLQERLAKLAGGVAVVKVGAASETELKERKMRIEDALA 399
>SYL_PYRHO (O58698) Leucyl-tRNA synthetase (EC 6.1.1.4)
(Leucine--tRNA ligase) (LeuRS)
Length = 967
Score = 29.3 bits (64), Expect = 1.7
Identities = 14/67 (20%), Positives = 35/67 (51%), Gaps = 1/67 (1%)
Query: 31 DQIEVTGEEIDSVKLASLLRKKFGYADLVSIEAVGKTEEKKDKEKAEAIVAWPYVYSSVP 90
++ ++ ++++ SL++ + GY D ++E V K K K+K + A +Y +
Sbjct: 347 EKYDIDPRIVENITYISLIKLE-GYGDFPAVEEVNKLGIKSQKDKEKLEQATKTIYKAEY 405
Query: 91 HYPVYEI 97
H ++++
Sbjct: 406 HKGIFKV 412
>RUBC_CHLRE (Q42695) RuBisCO subunit binding-protein beta-2 subunit
(60 kDa chaperonin beta-2 subunit) (CPN-60 beta-2)
(Fragment)
Length = 259
Score = 29.3 bits (64), Expect = 1.7
Identities = 23/88 (26%), Positives = 39/88 (44%), Gaps = 14/88 (15%)
Query: 4 QKLRNKAMAIAVGVS-GVEGTTIKGDNKDQIEVTG-------------EEIDSVKLASLL 49
+K + + +A VS E TTI GD + Q +V G +E + KL +
Sbjct: 25 EKATEEVLGLAAKVSISKEATTIVGDGRTQQQVEGRVKQIRNLAAETEQEYEKEKLNERI 84
Query: 50 RKKFGYADLVSIEAVGKTEEKKDKEKAE 77
+ G ++ + A +TE K+ K + E
Sbjct: 85 ARLSGGVAIIQVGAQTETELKEKKLRVE 112
>PEX3_PICAN (Q01497) Peroxisomal biogenesis factor 3 (Peroxin-3)
(Peroxisomal membrane protein PER9)
Length = 457
Score = 29.3 bits (64), Expect = 1.7
Identities = 21/54 (38%), Positives = 27/54 (49%), Gaps = 5/54 (9%)
Query: 1 MNNQKLRNKAMAIAVGVSGVEGTTI-----KGDNKDQIEVTGEEIDSVKLASLL 49
MN+Q ++ + VG+S V + K N DQ E EI VKLASLL
Sbjct: 362 MNDQVSAIISLLVTVGISKVLDNIVVNLMNKQKNPDQEEQVPTEIPKVKLASLL 415
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.310 0.129 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,849,069
Number of Sequences: 164201
Number of extensions: 429268
Number of successful extensions: 1216
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 47
Number of HSP's successfully gapped in prelim test: 21
Number of HSP's that attempted gapping in prelim test: 1169
Number of HSP's gapped (non-prelim): 68
length of query: 106
length of database: 59,974,054
effective HSP length: 82
effective length of query: 24
effective length of database: 46,509,572
effective search space: 1116229728
effective search space used: 1116229728
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144618.2