BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144516.12 - phase: 0
(105 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
RFX5_HUMAN (P48382) DNA-binding protein RFX5 (Regulatory factor ... 30 1.0
ERB2_HUMAN (P04626) Receptor tyrosine-protein kinase erbB-2 prec... 29 1.7
CH60_STAAW (P63767) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 29 2.3
CH60_STAAN (P99083) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 29 2.3
CH60_STAAM (P63766) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 29 2.3
CH60_STAAU (Q08854) 60 kDa chaperonin (Protein Cpn60) (groEL pro... 28 3.0
TAF4_DROME (P47825) Transcription initiation factor TFIID subuni... 28 3.9
SYW_HELHP (Q7VIP6) Tryptophanyl-tRNA synthetase (EC 6.1.1.2) (Tr... 28 3.9
BIMC_EMENI (P17120) Kinesin-like protein bimC 28 3.9
YM63_MYCBO (P64960) Hypothetical protein Mb2263c 28 5.1
YM39_MYCTU (P64959) Hypothetical protein Rv2239c/MT2299 28 5.1
SYFB_YEAST (P15624) Phenylalanyl-tRNA synthetase beta chain (EC ... 28 5.1
COAT_NODAV (P12871) Protein alpha [Contains: Coat protein beta (... 28 5.1
RIPA_HUMAN (Q9BXF6) Rab11 interacting protein Rip11 (Gamma-SNAP-... 27 6.6
PLS_STAEP (Q8CQD9) Accumulation-associated protein precursor 27 6.6
MTS1_MOUSE (Q8R1S4) Metastasis suppressor protein 1 (Missing in ... 27 6.6
AMOS_DROME (Q9Y0A7) Basic helix-loop-helix transcription factor ... 27 6.6
ISDF_RHILO (Q98MX9) IspD/ispF bifunctional enzyme [Includes: 2-C... 27 8.6
>RFX5_HUMAN (P48382) DNA-binding protein RFX5 (Regulatory factor X
subunit 5)
Length = 616
Score = 30.0 bits (66), Expect = 1.0
Identities = 19/61 (31%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Query: 13 QPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASNDKVT 72
+P+AK T A GGA A +PTT + G + K +N + + + NDK+
Sbjct: 5 EPDAKSPKTGGRAPPGGAEAGEPTTLLQRLRGTIS-KAVQNKVEGILQDVQKFSDNDKLY 63
Query: 73 L 73
L
Sbjct: 64 L 64
>ERB2_HUMAN (P04626) Receptor tyrosine-protein kinase erbB-2 precursor
(EC 2.7.1.112) (p185erbB2) (C-erbB-2) (NEU
proto-oncogene) (Tyrosine kinase-type cell surface
receptor HER2) (MLN 19)
Length = 1255
Score = 29.3 bits (64), Expect = 1.7
Identities = 19/63 (30%), Positives = 28/63 (44%), Gaps = 9/63 (14%)
Query: 13 QPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASNDKVT 72
QP + AA+ GA+ +P T +PG NG V+ VF FG + + +T
Sbjct: 1148 QPPSPREGPLPAARPAGATLERPKTLSPGKNGVVK---------DVFAFGGAVENPEYLT 1198
Query: 73 LAG 75
G
Sbjct: 1199 PQG 1201
>CH60_STAAW (P63767) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 538
Score = 28.9 bits (63), Expect = 2.3
Identities = 20/68 (29%), Positives = 32/68 (46%), Gaps = 3/68 (4%)
Query: 6 WRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVA 65
++K + E + T N + V+ E G+ G+V V+R KNA V G A
Sbjct: 421 YQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGV---GFNA 477
Query: 66 ASNDKVTL 73
A+N+ V +
Sbjct: 478 ATNEWVNM 485
>CH60_STAAN (P99083) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 538
Score = 28.9 bits (63), Expect = 2.3
Identities = 20/68 (29%), Positives = 32/68 (46%), Gaps = 3/68 (4%)
Query: 6 WRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVA 65
++K + E + T N + V+ E G+ G+V V+R KNA V G A
Sbjct: 421 YQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGV---GFNA 477
Query: 66 ASNDKVTL 73
A+N+ V +
Sbjct: 478 ATNEWVNM 485
>CH60_STAAM (P63766) 60 kDa chaperonin (Protein Cpn60) (groEL
protein)
Length = 538
Score = 28.9 bits (63), Expect = 2.3
Identities = 20/68 (29%), Positives = 32/68 (46%), Gaps = 3/68 (4%)
Query: 6 WRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVA 65
++K + E + T N + V+ E G+ G+V V+R KNA V G A
Sbjct: 421 YQKVSEIEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGV---GFNA 477
Query: 66 ASNDKVTL 73
A+N+ V +
Sbjct: 478 ATNEWVNM 485
>CH60_STAAU (Q08854) 60 kDa chaperonin (Protein Cpn60) (groEL
protein) (Heat shock protein 60)
Length = 538
Score = 28.5 bits (62), Expect = 3.0
Identities = 19/68 (27%), Positives = 32/68 (46%), Gaps = 3/68 (4%)
Query: 6 WRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVA 65
++K ++ E + T N + V+ E G+ G+V V+R KNA V G
Sbjct: 421 YQKVSENEAEGDIETGVNIVLKALTAPVRQIAENAGLEGSVIVERLKNAEPGV---GFNG 477
Query: 66 ASNDKVTL 73
A+N+ V +
Sbjct: 478 ATNEWVNM 485
>TAF4_DROME (P47825) Transcription initiation factor TFIID subunit 4
(Transcription initiation factor TFIID 110 kDa subunit)
(p110) (TAFII-110) (110 kDa TBP-associated factor)
Length = 921
Score = 28.1 bits (61), Expect = 3.9
Identities = 11/43 (25%), Positives = 20/43 (45%)
Query: 14 PEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASV 56
P +L T GG P+ + PG V+++ P++A +
Sbjct: 442 PNIRLPTAPRLVNTGGIRTQIPSLQVPGQANIVQIRGPQHAQL 484
>SYW_HELHP (Q7VIP6) Tryptophanyl-tRNA synthetase (EC 6.1.1.2)
(Tryptophan--tRNA ligase) (TrpRS)
Length = 332
Score = 28.1 bits (61), Expect = 3.9
Identities = 12/34 (35%), Positives = 21/34 (61%)
Query: 49 KRPKNASVSVFEFGSVAASNDKVTLAGYCPVSED 82
K PKN +V +F + ++ A++ + A + PV ED
Sbjct: 120 KNPKNINVGLFNYPALMAADILLYQADFVPVGED 153
>BIMC_EMENI (P17120) Kinesin-like protein bimC
Length = 1184
Score = 28.1 bits (61), Expect = 3.9
Identities = 16/41 (39%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Query: 19 STTENAAKNGGASAVKP--TTEAPGMNGAVEVKRPKNASVS 57
ST E + G+ V P TEA GMNG +R N+ V+
Sbjct: 1104 STGEKSTDQDGSPVVSPDSATEAEGMNGPPSKRRRSNSVVA 1144
>YM63_MYCBO (P64960) Hypothetical protein Mb2263c
Length = 158
Score = 27.7 bits (60), Expect = 5.1
Identities = 13/39 (33%), Positives = 17/39 (43%)
Query: 3 LLWWRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPG 41
LLWWR G + + A++G V P T PG
Sbjct: 78 LLWWRDGDGDLVDTLMDAIGPLAEDGVIWVVTPKTGQPG 116
>YM39_MYCTU (P64959) Hypothetical protein Rv2239c/MT2299
Length = 158
Score = 27.7 bits (60), Expect = 5.1
Identities = 13/39 (33%), Positives = 17/39 (43%)
Query: 3 LLWWRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPG 41
LLWWR G + + A++G V P T PG
Sbjct: 78 LLWWRDGDGDLVDTLMDAIGPLAEDGVIWVVTPKTGQPG 116
>SYFB_YEAST (P15624) Phenylalanyl-tRNA synthetase beta chain (EC
6.1.1.20) (Phenylalanine--tRNA ligase beta chain)
(PheRS)
Length = 594
Score = 27.7 bits (60), Expect = 5.1
Identities = 14/41 (34%), Positives = 20/41 (48%)
Query: 36 TTEAPGMNGAVEVKRPKNASVSVFEFGSVAASNDKVTLAGY 76
TT PG+ V+ R + + VFE G V +DK+ Y
Sbjct: 444 TTLLPGILKTVKENRKHSLPIKVFETGDVVFKDDKLERKAY 484
>COAT_NODAV (P12871) Protein alpha [Contains: Coat protein beta (EC
3.4.23.44) (Nodavirus endopeptidase); Coat protein
gamma]
Length = 399
Score = 27.7 bits (60), Expect = 5.1
Identities = 22/83 (26%), Positives = 39/83 (46%), Gaps = 10/83 (12%)
Query: 11 NSQPEAKLSTTENAAKNGG-----ASAVKPTTEAPGMNGAVE-----VKRPKNASVSVFE 60
N+Q + T + A+ G A A+ TT +N AV V+ N + +++E
Sbjct: 264 NAQASMFTNLTFSGARYTGLGDMDAIAILVTTPTGAVNTAVLKVWACVEYRPNPNSTLYE 323
Query: 61 FGSVAASNDKVTLAGYCPVSEDL 83
F + +ND+ LA Y ++ D+
Sbjct: 324 FARESPANDEYALAAYRKIARDI 346
>RIPA_HUMAN (Q9BXF6) Rab11 interacting protein Rip11
(Gamma-SNAP-associated factor-1) (Gaf-1) (Phosphoprotein
pp75)
Length = 653
Score = 27.3 bits (59), Expect = 6.6
Identities = 15/50 (30%), Positives = 25/50 (50%)
Query: 7 RKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASV 56
RK S LS+ K + +++P T+AP V+ KR K+++V
Sbjct: 541 RKQSRSSLSIALSSGLEKLKTVTSGSIQPVTQAPQAGQMVDTKRLKDSAV 590
>PLS_STAEP (Q8CQD9) Accumulation-associated protein precursor
Length = 1469
Score = 27.3 bits (59), Expect = 6.6
Identities = 23/88 (26%), Positives = 30/88 (33%), Gaps = 11/88 (12%)
Query: 10 KNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASND 69
+N E L T N EAP M+ ++ + NA + ND
Sbjct: 66 QNDSSERSLENTNQPTVNN---------EAPQMSSTLQAEEGSNAEAPNVPTIKANSDND 116
Query: 70 KVTLAGYCPVSEDLEPCRWEILPAVESN 97
T P DL R E +PAV N
Sbjct: 117 TQTQFSEAPTRNDL--ARKEDIPAVSKN 142
>MTS1_MOUSE (Q8R1S4) Metastasis suppressor protein 1 (Missing in
metastasis protein)
Length = 759
Score = 27.3 bits (59), Expect = 6.6
Identities = 19/71 (26%), Positives = 31/71 (42%), Gaps = 1/71 (1%)
Query: 5 WWRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVS-VFEFGS 63
W + G QP + ++ PTT GA E +RP++ +VS G
Sbjct: 416 WAKPGPYDQPLVNTLQRRKEKREPDSNGGGPTTTGGPPAGAEEAQRPRSMTVSAATRPGE 475
Query: 64 VAASNDKVTLA 74
A+ +++TLA
Sbjct: 476 EMAACEELTLA 486
>AMOS_DROME (Q9Y0A7) Basic helix-loop-helix transcription factor
Amos (Reduced olfactory organs protein) (Rough eye
protein) (Absent MD neurons and olfactory sensilla
protein) (Amos protein)
Length = 198
Score = 27.3 bits (59), Expect = 6.6
Identities = 14/46 (30%), Positives = 27/46 (58%), Gaps = 3/46 (6%)
Query: 5 WWRKGKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKR 50
+W K + S+P KLST+ +++ + +S+ ++ + G G V KR
Sbjct: 97 YWNKQQRSKPYDKLSTSMSSSTSSASSS---SSSSAGFGGEVLKKR 139
>ISDF_RHILO (Q98MX9) IspD/ispF bifunctional enzyme [Includes:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
(EC 2.7.7.60) (4-diphosphocytidyl-2C-methyl-D-erythritol
synthase) (MEP cytidylyltransferase) (MCT);
2-C-methyl-D-erythritol 2,4-cyclodi
Length = 407
Score = 26.9 bits (58), Expect = 8.6
Identities = 18/84 (21%), Positives = 32/84 (37%), Gaps = 8/84 (9%)
Query: 9 GKNSQPEAKLSTTENAAKNGGASAVKPTTEAPGMNGAVEVKRPKNASVSVFEFGSVAASN 68
G+N L + + A V T G++ A + P+ F +G + A++
Sbjct: 135 GENEGALPALPVADTLKRESAAGVVAETVSRSGLHAA---QTPQG-----FPYGPILAAH 186
Query: 69 DKVTLAGYCPVSEDLEPCRWEILP 92
DK G ++D W +P
Sbjct: 187 DKAFQLGRLDFTDDAAIAEWAHIP 210
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.311 0.127 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,794,719
Number of Sequences: 164201
Number of extensions: 451605
Number of successful extensions: 1019
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1011
Number of HSP's gapped (non-prelim): 18
length of query: 105
length of database: 59,974,054
effective HSP length: 81
effective length of query: 24
effective length of database: 46,673,773
effective search space: 1120170552
effective search space used: 1120170552
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144516.12