BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144513.13 + phase: 0
(544 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
PME_PRUPE (Q43062) Pectinesterase PPE8B precursor (EC 3.1.1.11) ... 465 e-130
PME2_CITSI (O04887) Pectinesterase 2 precursor (EC 3.1.1.11) (Pe... 416 e-116
PME1_CITSI (O04886) Pectinesterase 1 precursor (EC 3.1.1.11) (Pe... 393 e-109
PM21_LYCES (P09607) Pectinesterase 2 precursor (EC 3.1.1.11) (Pe... 392 e-108
PM22_LYCES (Q96575) Pectinesterase 2 precursor (EC 3.1.1.11) (Pe... 392 e-108
PME3_CITSI (P83948) Pectinesterase 3 precursor (EC 3.1.1.11) (Pe... 390 e-108
PME1_FICAW (P83947) Pectinesterase precursor (EC 3.1.1.11) (Pect... 390 e-108
PMEU_LYCES (Q43143) Pectinesterase U1 precursor (EC 3.1.1.11) (P... 389 e-107
PME1_LYCES (P14280) Pectinesterase 1 precursor (EC 3.1.1.11) (Pe... 384 e-106
PME3_PHAVU (Q43111) Pectinesterase 3 precursor (EC 3.1.1.11) (Pe... 381 e-105
PME2_ARATH (Q42534) Pectinesterase 2 precursor (EC 3.1.1.11) (Pe... 380 e-105
PME3_LYCES (Q96576) Pectinesterase 3 precursor (EC 3.1.1.11) (Pe... 366 e-101
PME1_ARATH (Q43867) Pectinesterase 1 precursor (EC 3.1.1.11) (Pe... 344 3e-94
PME_DAUCA (P83218) Pectinesterase (EC 3.1.1.11) (Pectin methyles... 336 1e-91
PME_PETIN (Q43043) Pectinesterase precursor (EC 3.1.1.11) (Pecti... 300 8e-81
PME_MEDSA (Q42920) Pectinesterase precursor (EC 3.1.1.11) (Pecti... 297 5e-80
PME_BRANA (P41510) Probable pectinesterase precursor (EC 3.1.1.1... 257 6e-68
PME_BRACM (Q42608) Pectinesterase (EC 3.1.1.11) (Pectin methyles... 249 1e-65
PME_ASPAC (Q12535) Pectinesterase precursor (EC 3.1.1.11) (Pecti... 131 5e-30
PME_ASPTU (P17872) Pectinesterase precursor (EC 3.1.1.11) (Pecti... 115 3e-25
>PME_PRUPE (Q43062) Pectinesterase PPE8B precursor (EC 3.1.1.11)
(Pectin methylesterase) (PE)
Length = 522
Score = 465 bits (1196), Expect = e-130
Identities = 233/478 (48%), Positives = 317/478 (65%), Gaps = 35/478 (7%)
Query: 80 TINEAIGAINNMTKISTFSVNN----REQLAIEDCKELLDFSVSELAWSLGEMRRIRA-G 134
++ + I A+ + I + N R AI DC +LLDFS EL WSL + +
Sbjct: 59 SLKDTIDAVQQVASILSQFANAFGDFRLANAISDCLDLLDFSADELNWSLSASQNQKGKN 118
Query: 135 DRTAQYEGNLEAWLSAALSNQDTCIEGFEGTDRRLESYISGSVTQVTQLISNVLSLYTQL 194
+ T + +L WLSAAL NQDTC GFEGT+ ++ IS + QVT L+ +L
Sbjct: 119 NSTGKLSSDLRTWLSAALVNQDTCSNGFEGTNSIVQGLISAGLGQVTSLVQELL------ 172
Query: 195 NRLPFRPPRNTTLHETSTDESL--EFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYR 252
T +H S + + P W+ D++LL++ G DA+VA DG+G +
Sbjct: 173 ----------TQVHPNSNQQGPNGQIPSWVKTKDRKLLQAD--GVSVDAIVAQDGTGNFT 220
Query: 253 TINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQG 312
+ +AV AAP +S RR+VIY+K+G YKEN+++KKK N+MM+GDG+ TI++ NR+F+ G
Sbjct: 221 NVTDAVLAAPDYSMRRYVIYIKRGTYKENVEIKKKKWNLMMIGDGMDATIISGNRSFVDG 280
Query: 313 WTTFRTATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYA 372
WTTFR+ATFAVSG+GFIA+D+TF NTAGP HQAVALR DSD S F+RC+I G QDTLY
Sbjct: 281 WTTFRSATFAVSGRGFIARDITFENTAGPEKHQAVALRSDSDLSVFYRCNIRGYQDTLYT 340
Query: 373 HSLRQFYRECEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFT 432
H++RQFYR+C+I GT+DFIFG+ V QNC+I + LP QK +ITAQGRK P++ TG +
Sbjct: 341 HTMRQFYRDCKISGTVDFIFGDATVVFQNCQILAKKGLPNQKNSITAQGRKDPNEPTGIS 400
Query: 433 IQDSYVLA----------SQPTYLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALD 482
IQ + A S PTYLGRPWK YSRTV + +++S++++P GWLEW G+FAL+
Sbjct: 401 IQFCNITADSDLEAASVNSTPTYLGRPWKLYSRTVIMQSFLSNVIRPEGWLEWNGDFALN 460
Query: 483 TLWYGEYRNYGPGSSLAGRVKWPGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTA 540
+L+YGEY NYGPG+ L RVKWPGY V +++ A +TV +F+ G WLP TGVK+TA
Sbjct: 461 SLFYGEYMNYGPGAGLGSRVKWPGYQVFNESTQAKNYTVAQFIEGNLWLPSTGVKYTA 518
>PME2_CITSI (O04887) Pectinesterase 2 precursor (EC 3.1.1.11)
(Pectin methylesterase) (PE)
Length = 510
Score = 416 bits (1070), Expect = e-116
Identities = 236/509 (46%), Positives = 302/509 (58%), Gaps = 40/509 (7%)
Query: 44 CMDIENQNSCLTNIHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKISTFSVNNRE 103
C N C + + T T +L+ + A A + + + N RE
Sbjct: 32 CGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLGSKCRNERE 91
Query: 104 QLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCIEGFE 163
+ A EDC+EL + +V +L + D+ + WLS AL+N +TC E
Sbjct: 92 KAAWEDCRELYELTVLKLNQTSNSSPGCTKVDK--------QTWLSTALTNLETCRASLE 143
Query: 164 GTDRRLESYISGSVTQ-VTQLISNVLSLYTQLNRLPFRPPRNTTLHETSTDESLEFPEWM 222
D + Y+ ++ VT+LISN LSL N++P+ P FP W+
Sbjct: 144 --DLGVPEYVLPLLSNNVTKLISNTLSL----NKVPYNEPSYKD----------GFPTWV 187
Query: 223 TEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENI 282
D++LL++ P I VVA DGSG +TI EAV AA R+VIY+K G Y ENI
Sbjct: 188 KPGDRKLLQTTPRANI---VVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENI 244
Query: 283 DMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMTFRNTAGPV 342
++K K NIM VGDGIG+TI+T +++ G TTF++AT AV G FIA+D+T RNTAGP
Sbjct: 245 EVKLK--NIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPN 302
Query: 343 NHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNC 402
NHQAVALR SD S F+RCS EG QDTLY HS RQFYREC+IYGT+DFIFGN A VLQNC
Sbjct: 303 NHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNC 362
Query: 403 KIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLA---------SQPTYLGRPWKEY 453
I+ R P P + T+TAQGR P+QSTG I + V A S T+LGRPWK+Y
Sbjct: 363 NIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQY 421
Query: 454 SRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDA 513
SRTVYI T++ S++ P GW+EW G+FAL+TL+Y EY N GPGSS A RVKW GYHV+
Sbjct: 422 SRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSP 481
Query: 514 SAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
S FTV F+ G SWLP T V FT+GL
Sbjct: 482 SQVSQFTVGNFIAGNSWLPATNVPFTSGL 510
>PME1_CITSI (O04886) Pectinesterase 1 precursor (EC 3.1.1.11)
(Pectin methylesterase) (PE)
Length = 584
Score = 393 bits (1010), Expect = e-109
Identities = 208/460 (45%), Positives = 295/460 (63%), Gaps = 19/460 (4%)
Query: 98 SVNNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDT 157
++ RE++A+ DC E +D ++ EL ++ ++ +Q+ +L+ +SAA++NQ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188
Query: 158 CIEGFEGTD--RRLESYISGSVTQVTQLISNVLSLY---TQLNRLPFRPPRNTTL-HETS 211
C++GF D + + +S V ++ SN L++ T + + R N L ETS
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETS 248
Query: 212 TDESLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVI 271
T + +P W++ D+ LL+S +AVVA DGSG ++T+ AV AAP +R++I
Sbjct: 249 TVDG--WPAWLSPGDRRLLQSS--SVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYII 304
Query: 272 YVKKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAK 331
+K G+Y+EN+++ KK NIM +GDG +TI+T +RN + G TTF++AT AV G+GF+A+
Sbjct: 305 RIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLAR 364
Query: 332 DMTFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFI 391
D+TF+NTAGP HQAVALRV +D SAF+ C + QDTLY HS RQF+ C I GT+DFI
Sbjct: 365 DITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFI 424
Query: 392 FGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLA---------SQ 442
FGN AAVLQNC I+ R P QK +TAQGR P+Q+TG IQ S + A S
Sbjct: 425 FGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSF 484
Query: 443 PTYLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRV 502
PTYLGRPWKEYSRTV + + ++ ++ P GW EW GNFAL+TL+YGE++N G G+ +GRV
Sbjct: 485 PTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRV 544
Query: 503 KWPGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
KW G+ VI A+ A FT F+ G SWL TG F+ GL
Sbjct: 545 KWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>PM21_LYCES (P09607) Pectinesterase 2 precursor (EC 3.1.1.11)
(Pectin methylesterase 2) (PE 2)
Length = 550
Score = 392 bits (1007), Expect = e-108
Identities = 216/516 (41%), Positives = 306/516 (58%), Gaps = 38/516 (7%)
Query: 44 CMDIENQNSCLTNIHNELTR---TGPPSPTSVINAALRTTINEAIGAINNMTKISTFSVN 100
C ++ CL+ + + ++ T S++ L ++++ AI + KI +
Sbjct: 54 CKTAQDSQLCLSYVSDLISNEIVTSDSDGLSILKKFLVYSVHQMNNAIPVVRKIKNQIND 113
Query: 101 NREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCIE 160
REQ A+ DC ELLD SV + S+ + + R+ N ++WLS L+N TC++
Sbjct: 114 IREQGALTDCLELLDLSVDLVCDSIAAIDK-----RSRSEHANAQSWLSGVLTNHVTCLD 168
Query: 161 GFEGTDRRLESYISGSVTQVTQLISNVLSLYTQLNRLPFRPPRNTTLHETSTDESL---- 216
+ + + I+G T + +LIS L + T DE L
Sbjct: 169 ELDSFTKAM---ING--TNLDELISRAKVALAMLASVT-----------TPNDEVLRPGL 212
Query: 217 -EFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKK 275
+ P W++ D++L++S A+AVVA DG+G+YRT+ EAV AAP S R+VIYVK+
Sbjct: 213 GKMPSWVSSRDRKLMESSGKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKR 272
Query: 276 GLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMTF 335
G YKEN+++ + N+M++GDG+ TI+T + N + G TTF +AT A GKGFI +D+
Sbjct: 273 GTYKENVEVSSRKMNLMIIGDGMYATIITGSLNVVDGSTTFHSATLAAVGKGFILQDICI 332
Query: 336 RNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNG 395
+NTAGP HQAVALRV +D+S RC I+ QDTLYAHS RQFYR+ + GTIDFIFGN
Sbjct: 333 QNTAGPAKHQAVALRVGADKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNA 392
Query: 396 AAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLASQ---------PTYL 446
A V Q C++ R P Q+ +TAQGR P+Q+TG +IQ ++AS PTYL
Sbjct: 393 AVVFQKCQLVARKPGKYQQNMVTAQGRTDPNQATGTSIQFCDIIASPDLKPVVKEFPTYL 452
Query: 447 GRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPG 506
GRPWK+YSRTV + +Y+ ++ P GW EW G+FAL TL+YGE+ N GPG+ + RVKWPG
Sbjct: 453 GRPWKKYSRTVVMESYLGGLIDPSGWAEWHGDFALKTLYYGEFMNNGPGAGTSKRVKWPG 512
Query: 507 YHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
YHVI D + A FTV + + GGSWL T V + GL
Sbjct: 513 YHVITDPAEAMSFTVAKLIQGGSWLRSTDVAYVDGL 548
>PM22_LYCES (Q96575) Pectinesterase 2 precursor (EC 3.1.1.11)
(Pectin methylesterase 2) (PE 2)
Length = 550
Score = 392 bits (1006), Expect = e-108
Identities = 217/513 (42%), Positives = 308/513 (59%), Gaps = 32/513 (6%)
Query: 44 CMDIENQNSCLTNIH----NELTRTGPPSPTSVINAALRTTINEAIGAINNMTKISTFSV 99
C ++ CL+ + NE+ T S++ L +++ AI ++K+
Sbjct: 54 CKTAQDSQLCLSYVSDLMSNEIVTTDSDG-LSILMKFLVNYVHQMNNAIPVVSKMKNQIN 112
Query: 100 NNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCI 159
+ R++ A+ DC ELLD SV ++ S+ + + RT N ++WLS L+N TC+
Sbjct: 113 DIRQEGALTDCLELLDQSVDLVSDSIAAIDK-----RTHSEHANAQSWLSGVLTNHVTCL 167
Query: 160 EGFEGTDRRLESYISGSVTQVTQLISNVLSLYTQLNRLPFRPPRNTTLHETSTDESL-EF 218
+ + + + I+G T + +LIS L + TT ++ L +
Sbjct: 168 DELDSFTKAM---ING--TNLDELISRAKVALAMLASV-------TTPNDDVLRPGLGKM 215
Query: 219 PEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLY 278
P W++ D++L++S A+AVVA DG+G+YRT+ EAV AAP S R+VIYVK+G+Y
Sbjct: 216 PSWVSSRDRKLMESSGKDIGANAVVAKDGTGKYRTLAEAVAAAPDKSKTRYVIYVKRGIY 275
Query: 279 KENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMTFRNT 338
KEN+++ + +M+VGDG+ TI+T N N + G TTF +AT A GKGFI +D+ +NT
Sbjct: 276 KENVEVSSRKMKLMIVGDGMHATIITGNLNVVDGSTTFHSATLAAVGKGFILQDICIQNT 335
Query: 339 AGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAV 398
AGP HQAVALRV +D+S RC I+ QDTLYAHS RQFYR+ + GTIDFIFGN A V
Sbjct: 336 AGPAKHQAVALRVGADKSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTIDFIFGNAAVV 395
Query: 399 LQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLASQ---------PTYLGRP 449
Q CK+ R P Q+ +TAQGR P+Q+TG +IQ ++AS PTYLGRP
Sbjct: 396 FQKCKLVARKPGKYQQNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPTYLGRP 455
Query: 450 WKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHV 509
WK+YSRTV + +Y+ ++ P GW EW G+FAL TL+YGE+ N GPG+ + RVKWPGYH
Sbjct: 456 WKKYSRTVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHC 515
Query: 510 IKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
I D + A FTV + + GGSWL TGV + GL
Sbjct: 516 ITDPAEAMPFTVAKLIQGGSWLRSTGVAYVDGL 548
>PME3_CITSI (P83948) Pectinesterase 3 precursor (EC 3.1.1.11)
(Pectin methylesterase 3) (PE 3)
Length = 584
Score = 390 bits (1003), Expect = e-108
Identities = 206/460 (44%), Positives = 294/460 (63%), Gaps = 19/460 (4%)
Query: 98 SVNNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDT 157
++ RE++A+ DC E +D ++ EL ++ ++ +Q+ +L+ +SAA++NQ T
Sbjct: 129 NLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGT 188
Query: 158 CIEGFEGTD--RRLESYISGSVTQVTQLISNVLSLY---TQLNRLPFRPPRNTTL-HETS 211
C++GF D + + +S V ++ SN L++ T + + R N L ETS
Sbjct: 189 CLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETS 248
Query: 212 TDESLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVI 271
T + +P W++ D+ LL+S + VVA DGSG ++T+ +V AAP +R++I
Sbjct: 249 TVDG--WPAWLSTGDRRLLQSS--SVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYII 304
Query: 272 YVKKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAK 331
+K G+Y+EN+++ KK NIM +GDG +TI+T +RN + G TTF++AT AV G+GF+A+
Sbjct: 305 RIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLAR 364
Query: 332 DMTFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFI 391
D+TF+NTAGP HQAVALRV +D SAF+ C + QDTLY HS RQF+ C I GT+DFI
Sbjct: 365 DITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFI 424
Query: 392 FGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLA---------SQ 442
FGN AAVLQNC I+ R P QK +TAQGR P+Q+TG IQ S + A S
Sbjct: 425 FGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSF 484
Query: 443 PTYLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRV 502
PTYLGRPWKEYSRTV + + ++ ++ P GW EW GNFAL+TL+YGE++N G G+ +GRV
Sbjct: 485 PTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRV 544
Query: 503 KWPGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
KW G+ VI A+ A FT F+ G SWL TG F+ GL
Sbjct: 545 KWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
>PME1_FICAW (P83947) Pectinesterase precursor (EC 3.1.1.11) (Pectin
methylesterase) (PE)
Length = 545
Score = 390 bits (1001), Expect = e-108
Identities = 217/533 (40%), Positives = 311/533 (57%), Gaps = 24/533 (4%)
Query: 20 VLSFPDEIPSDIQTQDMQALIAQACMDIENQNSCLTNIHNELTRTGPPSPTSVINAALRT 79
V+SF + + + ++ +L + C N+ SCL I +E+T +++ + L
Sbjct: 25 VISFAALVSTGFSSPEL-SLHHKICDQSVNKESCLAMI-SEVTGLNMADHRNLLKSFLEK 82
Query: 80 TINEAIGAINNMTKISTFSVNNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQ 139
T A S N +E+ A+ DC EL+D S + S+ + R+ +
Sbjct: 83 TTPRIQKAFETANDASRRINNPQERTALLDCAELMDLSKERVVDSISILFHQNLTTRSHE 142
Query: 140 YEGNLEAWLSAALSNQDTCIEGFE-GTDRRLESYISGSVTQVTQLISNVLSLYTQLNRLP 198
+L WLS L+N TC++G E G+ +++ + + ++ L+++ L
Sbjct: 143 ---DLHVWLSGVLTNHVTCLDGLEEGSTDYIKTLMESHLNELILRARTSLAIFVTLF--- 196
Query: 199 FRPPRNTTLHETSTDESLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAV 258
P + + E T FP W+T D+ LL++ D VVA DGSG Y T+NEAV
Sbjct: 197 ---PAKSNVIEPVTGN---FPTWVTAGDRRLLQTLGKDIEPDIVVAKDGSGDYETLNEAV 250
Query: 259 NAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRT 318
A P +S +R ++ V+ G+Y+EN+D + N+M+VG+G+ TI+T +RN + G TTF +
Sbjct: 251 AAIPDNSKKRVIVLVRTGIYEENVDFGYQKKNVMLVGEGMDYTIITGSRNVVDGSTTFDS 310
Query: 319 ATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQF 378
AT A G GFIA+D+ F+NTAGP +QAVALR+ +D++ RC I+ QDTLY H+ RQF
Sbjct: 311 ATVAAVGDGFIAQDICFQNTAGPEKYQAVALRIGADETVINRCRIDAYQDTLYPHNYRQF 370
Query: 379 YRECEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYV 438
YR+ I GT+DFIFGN A V QNC + R + Q+ TITAQGR P+Q+TG +IQ+ +
Sbjct: 371 YRDRNITGTVDFIFGNAAVVFQNCNLIPRKQMKGQENTITAQGRTDPNQNTGTSIQNCEI 430
Query: 439 LASQ---------PTYLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEY 489
AS +YLGRPWKEYSRTV + +Y+S ++ P GWLEW +FAL TL+YGEY
Sbjct: 431 FASADLEPVEDTFKSYLGRPWKEYSRTVVMESYISDVIDPAGWLEWDRDFALKTLFYGEY 490
Query: 490 RNYGPGSSLAGRVKWPGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
RN GPGS + RVKWPGYHVI A FTV + GGSWL TGV +TAGL
Sbjct: 491 RNGGPGSGTSERVKWPGYHVITSPEVAEQFTVAELIQGGSWLGSTGVDYTAGL 543
>PMEU_LYCES (Q43143) Pectinesterase U1 precursor (EC 3.1.1.11)
(Pectin methylesterase) (PE)
Length = 583
Score = 389 bits (999), Expect = e-107
Identities = 218/527 (41%), Positives = 318/527 (59%), Gaps = 29/527 (5%)
Query: 38 ALIAQACMDIENQNSCLTNIHNELTRTGP-PSPTSVINAALRTTINEA---IGAINNMTK 93
A++ AC + + C + I N + S VI +L T+ A+ + K
Sbjct: 64 AIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIK 123
Query: 94 ISTFSVNNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALS 153
+ RE++A+ DC E +D ++ EL ++ ++ ++ +L+ +S+A++
Sbjct: 124 TRK-GLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAIT 182
Query: 154 NQDTCIEGF--EGTDRRLESYISGSVTQVTQLISNVLSLYTQL------NRLPFRPPRNT 205
NQ+TC++GF + D+++ + V ++ SN L++ + N + P N
Sbjct: 183 NQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPAN- 241
Query: 206 TLHETSTDESLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHS 265
+ +++ E+PEW++ D+ LL+S D VVA DGSG Y+T++EAV AP S
Sbjct: 242 --NRKLVEDNGEWPEWLSAGDRRLLQSST--VTPDVVVAADGSGDYKTVSEAVRKAPEKS 297
Query: 266 NRRHVIYVKKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFA-VS 324
++R+VI +K G+Y+EN+D+ KK TNIM +GDG TI+T++RN G TTF +AT V+
Sbjct: 298 SKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVA 357
Query: 325 GKGFIAKDMTFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEI 384
GK +A+D+TF+NTAG HQAVAL V SD SAF+RC + QDTLY HS RQF+ +C +
Sbjct: 358 GK-VLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLV 416
Query: 385 YGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLA---- 440
GT+DFIFGNGAAV Q+C I+ R P QK +TAQGR P+Q+TG IQ + A
Sbjct: 417 AGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 476
Query: 441 -----SQPTYLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPG 495
S PTYLGRPWKEYSRTV + + ++ ++QP GW EW GNFALDTL+YGEY N G G
Sbjct: 477 RPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAG 536
Query: 496 SSLAGRVKWPGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
+ +GRVKW G+ VI ++ A +T RF+ GGSWL TG F+ GL
Sbjct: 537 APTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
>PME1_LYCES (P14280) Pectinesterase 1 precursor (EC 3.1.1.11)
(Pectin methylesterase 1) (PE 1)
Length = 546
Score = 384 bits (986), Expect = e-106
Identities = 216/518 (41%), Positives = 303/518 (57%), Gaps = 42/518 (8%)
Query: 44 CMDIENQNSCLTN----IHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKISTFSV 99
C ++ CL+ I NE+ T S++ L +++ AI + K+
Sbjct: 50 CKTAQDSQLCLSYVSDLISNEIVTTESDGH-SILMKFLVNYVHQMNNAIPVVRKMKNQIN 108
Query: 100 NNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCI 159
+ R+ A+ DC ELLD SV + S+ + + R+ N ++WLS L+N TC+
Sbjct: 109 DIRQHGALTDCLELLDQSVDFASDSIAAIDK-----RSRSEHANAQSWLSGVLTNHVTCL 163
Query: 160 EGFEGTDRRLESYISGSVTQVTQLISNVLSLYTQLNRLPFRPPRNTTLHETSTDESL--- 216
+ + + + I+G T + +LIS L L T+ DE +
Sbjct: 164 DELDSFTKAM---ING--TNLEELISRAKVALAMLASL------------TTQDEDVFMT 206
Query: 217 ---EFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYV 273
+ P W++ D++L++S IA+AVVA DG+G Y+T+ EAV AAP S R+VIYV
Sbjct: 207 VLGKMPSWVSSMDRKLMESSGKDIIANAVVAQDGTGDYQTLAEAVAAAPDKSKTRYVIYV 266
Query: 274 KKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDM 333
K+G YKEN+++ N+M+VGDG+ T +T + N + G TTFR+AT A G+GFI +D+
Sbjct: 267 KRGTYKENVEVASNKMNLMIVGDGMYATTITGSLNVVDGSTTFRSATLAAVGQGFILQDI 326
Query: 334 TFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFG 393
+NTAGP QAVALRV +D S RC I+ QDTLYAHS RQFYR+ + GT+DFIFG
Sbjct: 327 CIQNTAGPAKDQAVALRVGADMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFG 386
Query: 394 NGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLASQ---------PT 444
N A V Q C++ R P Q+ +TAQGR P+Q+TG +IQ ++AS PT
Sbjct: 387 NAAVVFQKCQLVARKPGKYQQNMVTAQGRTDPNQATGTSIQFCNIIASSDLEPVLKEFPT 446
Query: 445 YLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKW 504
YLGRPWKEYSRTV + +Y+ ++ P GW EW G+FAL TL+YGE+ N GPG+ + RVKW
Sbjct: 447 YLGRPWKEYSRTVVMESYLGGLINPAGWAEWDGDFALKTLYYGEFMNNGPGAGTSKRVKW 506
Query: 505 PGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
PGYHVI D + A FTV + + GGSWL TGV + GL
Sbjct: 507 PGYHVITDPAKAMPFTVAKLIQGGSWLRSTGVAYVDGL 544
>PME3_PHAVU (Q43111) Pectinesterase 3 precursor (EC 3.1.1.11)
(Pectin methylesterase 3) (PE 3)
Length = 581
Score = 381 bits (979), Expect = e-105
Identities = 217/515 (42%), Positives = 298/515 (57%), Gaps = 18/515 (3%)
Query: 38 ALIAQACMDIENQNSCLTNIHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKISTF 97
A + C +SC ++I + L + P + +LR I+E + + +
Sbjct: 75 ASLKAVCDTTRYPSSCFSSI-SSLPESNTTDPELLFKLSLRVAIDELSSFPSKLR--ANA 131
Query: 98 SVNNREQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDT 157
+ R Q AI+ C + ++ L S+ + + ++ N+E WLSAAL++QDT
Sbjct: 132 EQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSAALTDQDT 191
Query: 158 CIEGFEGTD----RRLESYISGSVTQVTQLISNVLSLYTQLNRLPFRPPRNTTLHETSTD 213
C++ + R I ++ T+ SN L++ T++ L R T +H
Sbjct: 192 CLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSR--FETPIHHRRL- 248
Query: 214 ESLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYV 273
L FPEW+ A++ LL+ K + DAVVA DGSGQ++TI EA+ S R +YV
Sbjct: 249 --LGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSEERFSVYV 306
Query: 274 KKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDM 333
K+G Y ENID+ K N+M+ GDG +T V +RNFM G TF TATFAV GKGFIAKD+
Sbjct: 307 KEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGKGFIAKDI 366
Query: 334 TFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFG 393
F N AG HQAVALR SD+S FFRCS +G QDTLYAHS RQFYR+C+I GTIDFIFG
Sbjct: 367 GFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITGTIDFIFG 426
Query: 394 NGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVL-----ASQPTYLGR 448
N A V Q+CKI R PLP Q TITAQG+K P+Q+TG IQ S + + PTYLGR
Sbjct: 427 NAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLTAPTYLGR 486
Query: 449 PWKEYSRTVYINTYMSSMVQPRGWLEWLGNF-ALDTLWYGEYRNYGPGSSLAGRVKWPGY 507
PWK++S TV + + + +++ P GW+ W+ N T++Y EY+N GPG+ ++ RVKW GY
Sbjct: 487 PWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQRVKWAGY 546
Query: 508 HVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
A FTVQ F+ G WLP V+F + L
Sbjct: 547 KPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581
>PME2_ARATH (Q42534) Pectinesterase 2 precursor (EC 3.1.1.11)
(Pectin methylesterase 2) (PE 2)
Length = 587
Score = 380 bits (976), Expect = e-105
Identities = 207/465 (44%), Positives = 288/465 (61%), Gaps = 26/465 (5%)
Query: 102 REQLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCIEG 161
RE A+ DC E +D ++ EL ++ ++ + ++ +L+ +S+A++NQ TC++G
Sbjct: 125 REVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDG 184
Query: 162 F--EGTDRRLESYISGSVTQVTQLISNVLSLYTQLNRLP------------FRPPRNTTL 207
F + DR++ + V + SN L++ + F N L
Sbjct: 185 FSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKL 244
Query: 208 HETSTD-ESLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSN 266
E + D +S +P+W++ D+ LL+ ADA VA DGSG + T+ AV AAP SN
Sbjct: 245 KEVTGDLDSDGWPKWLSVGDRRLLQGSTIK--ADATVADDGSGDFTTVAAAVAAAPEKSN 302
Query: 267 RRHVIYVKKGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGK 326
+R VI++K G+Y+EN+++ KK TNIM +GDG G+TI+T +RN + G TTF +AT A G+
Sbjct: 303 KRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGE 362
Query: 327 GFIAKDMTFRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYG 386
F+A+D+TF+NTAGP HQAVALRV SD SAF++C + QDTLY HS RQF+ +C I G
Sbjct: 363 RFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITG 422
Query: 387 TIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQD------SYVLA 440
T+DFIFGN AAVLQ+C I R P QK +TAQGR P+Q+TG IQ+ S +LA
Sbjct: 423 TVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLA 482
Query: 441 SQ---PTYLGRPWKEYSRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSS 497
+ PTYLGRPWKEYSRTV + + +S +++P GW EW G+FALDTL Y EY N G G+
Sbjct: 483 VKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAG 542
Query: 498 LAGRVKWPGYHVIKDASAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
A RVKW GY VI + A FT +F+ GG WL TG F+ L
Sbjct: 543 TANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
>PME3_LYCES (Q96576) Pectinesterase 3 precursor (EC 3.1.1.11)
(Pectin methylesterase 3) (PE 3)
Length = 544
Score = 366 bits (939), Expect = e-101
Identities = 210/509 (41%), Positives = 298/509 (58%), Gaps = 30/509 (5%)
Query: 44 CMDIENQNSCLTNIHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKISTFSVNNRE 103
C ++ CL+ + +E+ T T V+ L +++ AI + KI + R+
Sbjct: 54 CKTAQDSQLCLSYV-SEIVTTESDGVT-VLKKFLVKYVHQMNNAIPVVRKIKNQINDIRQ 111
Query: 104 QLAIEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCIEGFE 163
Q A+ DC ELLD SV ++ S+ + + R+ N ++WLS L+N TC++
Sbjct: 112 QGALTDCLELLDQSVDLVSDSIAAIDK-----RSRSEHANAQSWLSGVLTNHVTCLDELT 166
Query: 164 GTDRRLESYISGSVTQVTQLISNVLSLYTQLNRLPFRPPRNTTLHETSTDESL-EFPEWM 222
S + + T + +LI+ L + TT ++ + L + P W+
Sbjct: 167 SF-----SLSTKNGTVLDELITRAKVALAMLASV-------TTPNDEVLRQGLGKMPYWV 214
Query: 223 TEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENI 282
+ D++L++S IA+ VVA DG+G Y+T+ EAV AAP + R+VIYVK G+YKEN+
Sbjct: 215 SSRDRKLMESSGKDIIANRVVAQDGTGDYQTLAEAVAAAPDKNKTRYVIYVKMGIYKENV 274
Query: 283 DMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMTFRNTAGPV 342
+ KK N+M+VGDG+ TI+T + N + G +TF + T A G+GFI +D+ +NTAGP
Sbjct: 275 VVTKKKMNLMIVGDGMNATIITGSLNVVDG-STFPSNTLAAVGQGFILQDICIQNTAGPE 333
Query: 343 NHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNC 402
QAVALRV +D S RC I+ QDTLYAHS RQFYR+ + GT+DFIFGN A V Q C
Sbjct: 334 KDQAVALRVGADMSVINRCRIDAYQDTLYAHSQRQFYRDSYVTGTVDFIFGNAAVVFQKC 393
Query: 403 KIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLAS---------QPTYLGRPWKEY 453
+I R P QK +TAQGR P+Q+TG +IQ ++AS TYLGRPWK++
Sbjct: 394 QIVARKPNKRQKNMVTAQGRTDPNQATGTSIQFCDIIASPDLEPVMNEYKTYLGRPWKKH 453
Query: 454 SRTVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDA 513
SRTV + +Y+ + P GW EW G+FAL TL+YGE+ N GPG+ + RVKWPGYHVI D
Sbjct: 454 SRTVVMQSYLDGHIDPSGWFEWRGDFALKTLYYGEFMNNGPGAGTSKRVKWPGYHVITDP 513
Query: 514 SAAGYFTVQRFLNGGSWLPRTGVKFTAGL 542
+ A FTV + GGSWL T V + GL
Sbjct: 514 NEAMPFTVAELIQGGSWLNSTSVAYVEGL 542
>PME1_ARATH (Q43867) Pectinesterase 1 precursor (EC 3.1.1.11)
(Pectin methylesterase 1) (PE 1)
Length = 586
Score = 344 bits (883), Expect = 3e-94
Identities = 196/507 (38%), Positives = 294/507 (57%), Gaps = 32/507 (6%)
Query: 52 SCLTNIHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKISTFSVNNREQLAIEDCK 111
SC+++I ++L + P ++ +L+ I+E + K+S + + R + A+ C
Sbjct: 85 SCISSI-SKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLSKETEDERIKSALRVCG 143
Query: 112 ELLDFSVSELAWSLG-----EMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCIEGFEGTD 166
+L++ ++ L ++ E ++ + + +L+ WLSA +++ +TC + +
Sbjct: 144 DLIEDALDRLNDTVSAIDDEEKKKTLSSSKIE----DLKTWLSATVTDHETCFDSLDELK 199
Query: 167 RRLESYISGSVTQ--------VTQLISNVLSLYTQ----LNRLPFRPPRNTTLHETSTDE 214
+ Y + ++TQ T+ SN L++ ++ L+ L R L +
Sbjct: 200 QNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPIHRRRRLMSHHHQQ 259
Query: 215 SLEFPEWMTEADQELLKSKPHGKIADAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVK 274
S++F +W A + LL++ G D VA DG+G T+NEAV P S + VIYVK
Sbjct: 260 SVDFEKW---ARRRLLQTA--GLKPDVTVAGDGTGDVLTVNEAVAKVPKKSLKMFVIYVK 314
Query: 275 KGLYKENIDMKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMT 334
G Y EN+ M K N+M+ GDG G+TI++ ++NF+ G T+ TATFA+ GKGFI KD+
Sbjct: 315 SGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQGKGFIMKDIG 374
Query: 335 FRNTAGPVNHQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGN 394
NTAG HQAVA R SD S +++CS +G QDTLY HS RQFYR+C++ GTIDFIFG+
Sbjct: 375 IINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDVTGTIDFIFGS 434
Query: 395 GAAVLQNCKIYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYVLAS----QPTYLGRPW 450
A V Q CKI R PL Q TITAQG+K P+QS+G +IQ + A+ PTYLGRPW
Sbjct: 435 AAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNVIAPTYLGRPW 494
Query: 451 KEYSRTVYINTYMSSMVQPRGWLEWL-GNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHV 509
KE+S TV + T + ++V+P GW+ W+ G ++ YGEY+N GPGS + RVKW GY
Sbjct: 495 KEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDVTQRVKWAGYKP 554
Query: 510 IKDASAAGYFTVQRFLNGGSWLPRTGV 536
+ + A FTV L+G W+P TGV
Sbjct: 555 VMSDAEAAKFTVATLLHGADWIPATGV 581
>PME_DAUCA (P83218) Pectinesterase (EC 3.1.1.11) (Pectin
methylesterase) (PE)
Length = 319
Score = 336 bits (861), Expect = 1e-91
Identities = 168/312 (53%), Positives = 215/312 (68%), Gaps = 9/312 (2%)
Query: 240 DAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVGDGIG 299
+ VVA DGSG Y+T++EAV AAP S R+VI +K G+Y+EN+D+ KK NIM +GDG
Sbjct: 8 NVVVAADGSGDYKTVSEAVAAAPEDSKTRYVIRIKAGVYRENVDVPKKKKNIMFLGDGRT 67
Query: 300 QTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVDSDQSAFF 359
TI+T+++N G TTF +AT A G GF+A+D+TF+NTAG HQAVALRV SD SAF+
Sbjct: 68 STIITASKNVQDGSTTFNSATVAAVGAGFLARDITFQNTAGAAKHQAVALRVGSDLSAFY 127
Query: 360 RCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITA 419
RC I QD+LY HS RQF+ C I GT+DFIFGN A VLQ+C I+ R P QK +TA
Sbjct: 128 RCDILAYQDSLYVHSNRQFFINCFIAGTVDFIFGNAAVVLQDCDIHARRPGSGQKNMVTA 187
Query: 420 QGRKSPHQSTGFTIQDSY---------VLASQPTYLGRPWKEYSRTVYINTYMSSMVQPR 470
QGR P+Q+TG IQ S V +S PTYLGRPWKEYSRTV + + +++++ P
Sbjct: 188 QGRTDPNQNTGIVIQKSRIGATSDLQPVQSSFPTYLGRPWKEYSRTVVMQSSITNVINPA 247
Query: 471 GWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDASAAGYFTVQRFLNGGSW 530
GW W GNFALDTL+YGEY+N G G++ +GRV W G+ VI ++ A FT F+ GGSW
Sbjct: 248 GWFPWDGNFALDTLYYGEYQNTGAGAATSGRVTWKGFKVITSSTEAQGFTPGSFIAGGSW 307
Query: 531 LPRTGVKFTAGL 542
L T F+ GL
Sbjct: 308 LKATTFPFSLGL 319
>PME_PETIN (Q43043) Pectinesterase precursor (EC 3.1.1.11) (Pectin
methylesterase) (PE)
Length = 374
Score = 300 bits (767), Expect = 8e-81
Identities = 146/314 (46%), Positives = 201/314 (63%), Gaps = 11/314 (3%)
Query: 240 DAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVGDGIG 299
+A VALDGSGQY+TI EA++A P + +I++K G+YKE ID+ K MTN++++G+G
Sbjct: 58 NATVALDGSGQYKTIKEALDAVPKKNTEPFIIFIKAGVYKEYIDIPKSMTNVVLIGEGPT 117
Query: 300 QTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVDSDQSAFF 359
+T +T N++ G +TF T T V+G F+AK++ F NTAGP QAVALRV +D++ +
Sbjct: 118 KTKITGNKSVKDGPSTFHTTTVGVNGANFVAKNIGFENTAGPEKEQAVALRVSADKAIIY 177
Query: 360 RCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITA 419
C I+G QDTLY H+ RQFYR+C I GT+DFIFGNG AVLQNCK+ R P Q +TA
Sbjct: 178 NCQIDGYQDTLYVHTYRQFYRDCTITGTVDFIFGNGEAVLQNCKVIVRKPAQNQSCMVTA 237
Query: 420 QGRKSPHQSTGFTIQD-------SYVLASQP--TYLGRPWKEYSRTVYINTYMSSMVQPR 470
QGR P Q +Q+ Y S P TYLGRPWKEYSRT+ + +Y+ ++P
Sbjct: 238 QGRTEPIQKGAIVLQNCEIKPDTDYFSLSPPSKTYLGRPWKEYSRTIIMQSYIDKFIEPE 297
Query: 471 GWLEW-LGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDASAAGYFTVQRFLNGG- 528
GW W + NF DT +Y EY+N GPG++L R+ W G+ AA FT ++N
Sbjct: 298 GWAPWNITNFGRDTSYYAEYQNRGPGAALDKRITWKGFQKGFTGEAAQKFTAGVYINNDE 357
Query: 529 SWLPRTGVKFTAGL 542
+WL + V + AG+
Sbjct: 358 NWLQKANVPYEAGM 371
>PME_MEDSA (Q42920) Pectinesterase precursor (EC 3.1.1.11) (Pectin
methylesterase) (PE) (P65)
Length = 447
Score = 297 bits (760), Expect = 5e-80
Identities = 159/451 (35%), Positives = 259/451 (57%), Gaps = 22/451 (4%)
Query: 107 IEDCKELLDFSVSELAWSLGEMRRIRAGDRTAQYEGNLEAWLSAALSNQDTCIEGFEGTD 166
+E C E+LD++V + S+G + + + ++Y +L+ WL+ LS+Q TC++GF T
Sbjct: 1 MEICNEVLDYAVDGIHKSVGTLDQFDF-HKLSEYAFDLKVWLTGTLSHQQTCLDGFANTT 59
Query: 167 RRLESYISGSVTQVTQLISNVLSLYTQLNRL--PFRPPRNTTLHETSTDESLEFPEWMTE 224
+ ++ + +L SN + + ++R+ F + + +D+ + P W+ +
Sbjct: 60 TKAGETMTKVLKTSMELSSNAIDMMDAVSRILKGFDTSQYSVSRRLLSDDGI--PSWVND 117
Query: 225 ADQELLKSKPHGKIA-DAVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENID 283
+ LL G + +AVVA DGSGQ++T+ +A+ P + VI+VK G+YKE ++
Sbjct: 118 GHRRLLAG---GNVQPNAVVAQDGSGQFKTLTDALKTVPPKNAVPFVIHVKAGVYKETVN 174
Query: 284 MKKKMTNIMMVGDGIGQTIVTSNRNFMQGWTTFRTATFAVSGKGFIAKDMTFRNTAGPVN 343
+ K+M + ++GDG +T T + N+ G T+ TATF V+G F+AKD+ F NTAG
Sbjct: 175 VAKEMNYVTVIGDGPTKTKFTGSLNYADGINTYNTATFGVNGANFMAKDIGFENTAGTGK 234
Query: 344 HQAVALRVDSDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNCK 403
HQAVALRV +DQ+ F+ C ++G QDTLY S RQFYR+C I GTIDF+FG V QNCK
Sbjct: 235 HQAVALRVTADQAIFYNCQMDGFQDTLYVQSQRQFYRDCSISGTIDFVFGERFGVFQNCK 294
Query: 404 IYTRVPLPLQKVTITAQGRKSPHQSTGFTIQDSYV--------LASQPTYLGRPWKEYSR 455
+ R+P Q+ +TA GR+ + ++ Q S+ + + +YLGRPWK+YS+
Sbjct: 295 LVCRLPAKGQQCLVTAGGREKQNSASALVFQSSHFTGEPALTSVTPKVSYLGRPWKQYSK 354
Query: 456 TVYINTYMSSMVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDASA 515
V +++ + ++ P G++ W+G+ +T Y EY N GPG+ RVKW G V+ A
Sbjct: 355 VVIMDSTIDAIFVPEGYMPWMGSAFKETCTYYEYNNKGPGADTNLRVKWHGVKVLTSNVA 414
Query: 516 AGY-----FTVQRFLNGGSWLPRTGVKFTAG 541
A Y F + +W+ ++GV ++ G
Sbjct: 415 AEYYPGKFFEIVNATARDTWIVKSGVPYSLG 445
>PME_BRANA (P41510) Probable pectinesterase precursor (EC 3.1.1.11)
(Pectin methylesterase) (PE)
Length = 584
Score = 257 bits (656), Expect = 6e-68
Identities = 176/557 (31%), Positives = 269/557 (47%), Gaps = 66/557 (11%)
Query: 37 QALIAQACMDIENQNSCLTNIHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKIST 96
Q + C ++ SC L P+ +I A + T +A+ N T +
Sbjct: 43 QKAVESLCASATDKGSCAKT----LDPVKSDDPSKLIKAFMLAT-KDAVTKSTNFTASTE 97
Query: 97 F----SVNNREQLAIEDCKELLDFSVSELAWSLGEMRRI--RAGDRTAQYEGNLEAWLSA 150
++N + ++ CK +L +++ +L + EM ++G + Q L+ WL+
Sbjct: 98 EGMGKNINATSKAVLDYCKRVLMYALEDLETIVEEMGEDLQQSGSKMDQ----LKQWLTG 153
Query: 151 ALSNQDTCIEGFEGTDRRLESYISGSVTQVTQLISNVLSLY-------TQLN-------- 195
+ Q CI+ E ++ L + + L SN + ++ +Q+N
Sbjct: 154 VFNYQTDCIDDIEESE--LRKVMGEGIAHSKILSSNAIDIFHALTTAMSQMNVKVDDMKK 211
Query: 196 -RLPFRPPRNTTLHETSTDESLEFPEWMTEADQELLKSKPH----------------GKI 238
L P + L E + L P+W ++ D++L+ GKI
Sbjct: 212 GNLGETPAPDRDLLEDLDQKGL--PKWHSDKDRKLMAQAGRPGAPADEGIGEGGGGGGKI 269
Query: 239 ADA-VVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVGDG 297
VVA DGSGQ++TI+EAV A P + R +IY+K G+YKE + + KK+ N+ M GDG
Sbjct: 270 KPTHVVAKDGSGQFKTISEAVKACPEKNPGRCIIYIKAGVYKEQVTIPKKVNNVFMFGDG 329
Query: 298 IGQTIVTSNRN--FMQGWTTFRTATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVDSDQ 355
QTI+T +R+ G TT + T V +GF+AK + F+NTAGP+ HQAVA RV+ D+
Sbjct: 330 ATQTIITFDRSVGLSPGTTTSLSGTVQVESEGFMAKWIGFQNTAGPLGHQAVAFRVNGDR 389
Query: 356 SAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKV 415
+ F C +G QDTLY ++ RQFYR + GT+DFIFG A V+QN I R P Q
Sbjct: 390 AVIFNCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFIFGKSATVIQNSLILCRKGSPGQTN 449
Query: 416 TITAQG-RKSPHQSTGFTIQDSYVLASQ---------PTYLGRPWKEYSRTVYINTYMSS 465
+TA G K G + + ++A + +YLGRPWK ++ T I T +
Sbjct: 450 HVTADGNEKGKAVKIGIVLHNCRIMADKELEADRLTVKSYLGRPWKPFATTAVIGTEIGD 509
Query: 466 MVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDASAAGYFTVQRFL 525
++QP GW EW G T Y E+ N GPG++ A RV W + K A+ FTV +L
Sbjct: 510 LIQPTGWNEWQGEKFHLTATYVEFNNRGPGANTAARVPWA--KMAKSAAEVERFTVANWL 567
Query: 526 NGGSWLPRTGVKFTAGL 542
+W+ V GL
Sbjct: 568 TPANWIQEANVPVQLGL 584
>PME_BRACM (Q42608) Pectinesterase (EC 3.1.1.11) (Pectin
methylesterase) (PE) (Fragment)
Length = 571
Score = 249 bits (637), Expect = 1e-65
Identities = 174/557 (31%), Positives = 269/557 (48%), Gaps = 66/557 (11%)
Query: 37 QALIAQACMDIENQNSCLTNIHNELTRTGPPSPTSVINAALRTTINEAIGAINNMTKIST 96
Q + C ++ SC L P+ +I A + T +A+ N T +
Sbjct: 30 QKAVESLCASATDKGSCAKT----LDPVKSDDPSKLIKAFMLAT-KDAVTKSTNFTASTE 84
Query: 97 F----SVNNREQLAIEDCKELLDFSVSELAWSLGEMRRI--RAGDRTAQYEGNLEAWLSA 150
++N + ++ CK +L +++ +L + EM ++G + Q L+ WL+
Sbjct: 85 EGMGKNMNATSKAVLDYCKRVLMYALEDLETIVEEMGEDLQQSGSKMDQ----LKQWLTG 140
Query: 151 ALSNQDTCIEGFEGTDRRLESYISGSVTQVTQLISNVLSLY-------TQLN-------- 195
+ Q CI+ E ++ L + + L SN + ++ +Q+N
Sbjct: 141 VFNYQTDCIDDIEESE--LRKVMGEGIRHSKILSSNAIDIFHALTTAMSQMNVKVDDMKK 198
Query: 196 -RLPFRPPRNTTLHETSTDESLEFPEWMTEADQELLKSKPH----------------GKI 238
L P + L E + L P+W ++ D++L+ GKI
Sbjct: 199 GNLGETPAPDRDLLEDLDQKGL--PKWHSDKDRKLMAQAGRPGAPADEGIGEGGGGGGKI 256
Query: 239 ADA-VVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVGDG 297
VVA DGSGQ++TI+EAV A P + R +IY+K G+YKE + + KK+ N+ M GDG
Sbjct: 257 KPTHVVAKDGSGQFKTISEAVKACPEKNPGRCIIYIKAGVYKEQVTIPKKVNNVFMFGDG 316
Query: 298 IGQTIVTSNRN--FMQGWTTFRTATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVDSDQ 355
QTI+T +R+ G TT + T V +GF+AK + F+NTAGP+ +QAVA RV+ D+
Sbjct: 317 ATQTIITFDRSVGLSPGTTTSLSGTVQVESEGFMAKWIGFQNTAGPLGNQAVAFRVNGDR 376
Query: 356 SAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKV 415
+ F C +G QDTLY ++ RQFYR + GT+DFI G A V+QN I R P Q
Sbjct: 377 AVIFNCRFDGYQDTLYVNNGRQFYRNIVVSGTVDFINGKSATVIQNSLILCRKGSPGQTN 436
Query: 416 TITAQGR-KSPHQSTGFTIQDSYVLASQ---------PTYLGRPWKEYSRTVYINTYMSS 465
+TA G+ K G + + ++A + +YLGRPWK ++ T I T +
Sbjct: 437 HVTADGKQKGKAVKIGIVLHNCRIMADKELEADRLTVKSYLGRPWKPFATTAVIGTEIGD 496
Query: 466 MVQPRGWLEWLGNFALDTLWYGEYRNYGPGSSLAGRVKWPGYHVIKDASAAGYFTVQRFL 525
++QP GW EW G T Y E+ N GPG++ A RV W + K A+ FTV +L
Sbjct: 497 LIQPTGWNEWQGEKFHLTATYVEFNNRGPGANPAARVPWA--KMAKSAAEVERFTVANWL 554
Query: 526 NGGSWLPRTGVKFTAGL 542
+W+ V GL
Sbjct: 555 TPANWIQEANVTVQLGL 571
>PME_ASPAC (Q12535) Pectinesterase precursor (EC 3.1.1.11) (Pectin
methylesterase) (PE)
Length = 331
Score = 131 bits (329), Expect = 5e-30
Identities = 88/273 (32%), Positives = 129/273 (47%), Gaps = 19/273 (6%)
Query: 241 AVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVG----- 295
A+V G Y TI +A++A + + I++++G Y E + + +++ G
Sbjct: 27 AIVVAKSGGDYTTIGDAIDALSTSTTDTQTIFIEEGTYDEQVYLPAMTGKVIIYGQTENT 86
Query: 296 DGIGQTIVTSNRNFMQ---GWTTFRTATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVD 352
D +VT G + TATF G ++ NT G HQA+AL
Sbjct: 87 DSYADNLVTITHAISYEDAGESDDLTATFRNKAVGSQVYNLNIANTCGQACHQALALSAW 146
Query: 353 SDQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGA-AVLQNCKIYTRVPLP 411
+DQ ++ C+ G QDTL A + Q Y I G +DFIFG A A QN I RV
Sbjct: 147 ADQQGYYGCNFTGYQDTLLAQTGNQLYINSYIEGAVDFIFGQHARAWFQNVDI--RVVEG 204
Query: 412 LQKVTITAQGRKSPHQSTGFTIQDSYVLASQ-------PTYLGRPWKEYSRTVYINTYMS 464
+ITA GR S ++ + I S V A + YLGRPW EY+R V+ T M+
Sbjct: 205 PTSASITANGRSSETDTSYYVINKSTVAAKEGDDVAEGTYYLGRPWSEYARVVFQQTSMT 264
Query: 465 SMVQPRGWLEWLGNFA-LDTLWYGEYRNYGPGS 496
+++ GW EW + + + +GEY N G GS
Sbjct: 265 NVINSLGWTEWSTSTPNTEYVTFGEYANTGAGS 297
>PME_ASPTU (P17872) Pectinesterase precursor (EC 3.1.1.11) (Pectin
methylesterase) (PE)
Length = 331
Score = 115 bits (288), Expect = 3e-25
Identities = 82/272 (30%), Positives = 121/272 (44%), Gaps = 18/272 (6%)
Query: 241 AVVALDGSGQYRTINEAVNAAPSHSNRRHVIYVKKGLYKENIDMKKKMTNIMMVGDGIGQ 300
A+V G Y TI+ AV+A + S I++++G Y E + + +++ G
Sbjct: 27 AIVVAKSGGDYDTISAAVDALSTTSTETQTIFIEEGSYDEQVYIPALSGKLIVYGQTEDT 86
Query: 301 TIVTSNRNFMQGWTTF-------RTATFAVSGKGFIAKDMTFRNTAGPVNHQAVALRVDS 353
T TSN + TAT +G ++ NT G HQA+A+ +
Sbjct: 87 TTYTSNLVNITHAIALADVDNDDETATLRNYAEGSAIYNLNIANTCGQACHQALAVSAYA 146
Query: 354 DQSAFFRCSIEGNQDTLYAHSLRQFYRECEIYGTIDFIFGNGA-AVLQNCKIYTRVPLPL 412
+ ++ C G QDTL A + Q Y I G +DFIFG A A C I RV
Sbjct: 147 SEQGYYACQFTGYQDTLLAETGYQVYAGTYIEGAVDFIFGQHARAWFHECDI--RVLEGP 204
Query: 413 QKVTITAQGRKSPHQSTGFTIQDSYVLA-------SQPTYLGRPWKEYSRTVYINTYMSS 465
+ITA GR S + + I S V A S YLGRPW +Y+R + T M+
Sbjct: 205 SSASITANGRSSESDDSYYVIHKSTVAAADGNDVSSGTYYLGRPWSQYARVCFQKTSMTD 264
Query: 466 MVQPRGWLEWLGNFA-LDTLWYGEYRNYGPGS 496
++ GW EW + + + + EY N G G+
Sbjct: 265 VINHLGWTEWSTSTPNTENVTFVEYGNTGTGA 296
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.319 0.133 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,248,783
Number of Sequences: 164201
Number of extensions: 2564493
Number of successful extensions: 6038
Number of sequences better than 10.0: 37
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 5936
Number of HSP's gapped (non-prelim): 39
length of query: 544
length of database: 59,974,054
effective HSP length: 115
effective length of query: 429
effective length of database: 41,090,939
effective search space: 17628012831
effective search space used: 17628012831
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 68 (30.8 bits)
Medicago: description of AC144513.13