BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144481.8 + phase: 0 /pseudo
(119 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
Y492_MYCTU (Q11157) Hypothetical GMC-type oxidoreductase Rv0492c... 45 2e-05
FIXC_RHIME (P09820) FixC protein 43 1e-04
FIXC_ECOLI (P31575) FixC protein 42 3e-04
YDIS_ECOLI (P77337) Probable electron transfer flavoprotein-quin... 41 4e-04
Y4NJ_RHISN (P55582) Hypothetical GMC-type oxidoreductase Y4NJ 41 4e-04
FIXC_RHISN (Q53208) FixC protein 41 5e-04
AOFB_HUMAN (P27338) Amine oxidase [flavin-containing] B (EC 1.4.... 40 7e-04
FIXC_BRAJA (P10331) FixC protein 40 0.001
AOFB_CAVPO (P58028) Amine oxidase [flavin-containing] B (EC 1.4.... 40 0.001
FIXC_AZOCA (P26484) FixC protein 40 0.001
CRTI_PHYBL (P54982) Phytoene dehydrogenase (EC 1.14.99.-) (Phyto... 39 0.002
FIXC_AZOVI (P53572) FixC protein 39 0.003
AOFB_RAT (P19643) Amine oxidase [flavin-containing] B (EC 1.4.3.... 38 0.004
DLDH_STAAM (Q59822) Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (E3... 37 0.008
THI4_METJA (Q58018) Putative thiazole biosynthetic enzyme 37 0.010
THI4_ARCFU (O29556) Putative thiazole biosynthetic enzyme 37 0.010
THI4_AERPE (Q9Y9Z0) Putative thiazole biosynthetic enzyme 37 0.010
MDL2_PRUSE (O50048) (R)-mandelonitrile lyase 2 precursor (EC 4.1... 36 0.014
FIG1_HUMAN (Q96RQ9) Interleukin-4 induced protein 1 precursor (F... 36 0.014
YOR3_METBA (P96800) Hypothetical 31.9 kDa protein in HDRD 3'regi... 36 0.018
>Y492_MYCTU (Q11157) Hypothetical GMC-type oxidoreductase
Rv0492c/MT0511/MT0512
Length = 629
Score = 45.4 bits (106), Expect = 2e-05
Identities = 21/43 (48%), Positives = 29/43 (66%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSS 114
+ D V+VGSG GG + A LA +GL V+VLE+G +T +F S
Sbjct: 146 RADAVVVGSGAGGAMVARTLARAGLDVVVLEEGRRWTVEEFRS 188
>FIXC_RHIME (P09820) FixC protein
Length = 435
Score = 43.1 bits (100), Expect = 1e-04
Identities = 20/37 (54%), Positives = 25/37 (67%)
Query: 70 KVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
K K D ++VG+G G AA +AS GLKVL LE+G Y
Sbjct: 3 KEKFDAIVVGAGMSGNAAAYAMASRGLKVLQLERGEY 39
>FIXC_ECOLI (P31575) FixC protein
Length = 428
Score = 41.6 bits (96), Expect = 3e-04
Identities = 19/32 (59%), Positives = 24/32 (74%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
D +IVG+G G VAA VLA G +VLV+E+GN
Sbjct: 7 DAIIVGAGLAGSVAALVLAREGAQVLVIERGN 38
>YDIS_ECOLI (P77337) Probable electron transfer flavoprotein-quinone
oxidoreductase ydiS (EC 1.5.5.-)
Length = 429
Score = 41.2 bits (95), Expect = 4e-04
Identities = 18/34 (52%), Positives = 26/34 (75%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
K D ++VG+G G VAA V+A +GL VLV+E+G+
Sbjct: 5 KFDAIVVGAGVAGSVAALVMARAGLDVLVIERGD 38
>Y4NJ_RHISN (P55582) Hypothetical GMC-type oxidoreductase Y4NJ
Length = 505
Score = 41.2 bits (95), Expect = 4e-04
Identities = 18/38 (47%), Positives = 28/38 (73%)
Query: 69 LKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
L+++ D VI+GSG GG A VL ++GL V++LE+G +
Sbjct: 11 LQLEADAVIIGSGAGGASVADVLTAAGLYVIMLEEGGH 48
>FIXC_RHISN (Q53208) FixC protein
Length = 435
Score = 40.8 bits (94), Expect = 5e-04
Identities = 18/33 (54%), Positives = 23/33 (69%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
D ++VG+G G AA +AS GLKVL LE+G Y
Sbjct: 7 DAIVVGAGMSGNAAAYTMASRGLKVLQLERGEY 39
>AOFB_HUMAN (P27338) Amine oxidase [flavin-containing] B (EC
1.4.3.4) (Monoamine oxidase) (MAO-B)
Length = 519
Score = 40.4 bits (93), Expect = 7e-04
Identities = 21/44 (47%), Positives = 27/44 (60%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSSR 115
KCDVV+VG G G AA +L SGL V+VLE + R ++ R
Sbjct: 3 KCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLR 46
>FIXC_BRAJA (P10331) FixC protein
Length = 435
Score = 40.0 bits (92), Expect = 0.001
Identities = 16/39 (41%), Positives = 26/39 (66%)
Query: 68 ILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
+++ + D ++VG+G G AA +A G+KVL LE+G Y
Sbjct: 1 MIEERFDAIVVGAGMAGNAAALTMAKQGMKVLQLERGEY 39
>AOFB_CAVPO (P58028) Amine oxidase [flavin-containing] B (EC
1.4.3.4) (Monoamine oxidase) (MAO-B)
Length = 519
Score = 40.0 bits (92), Expect = 0.001
Identities = 21/44 (47%), Positives = 27/44 (60%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSSR 115
KCDVV+VG G G AA +L SGL V+VLE + R ++ R
Sbjct: 3 KCDVVVVGGGISGLAAAKLLHDSGLNVVVLEARDCVGGRTYTLR 46
>FIXC_AZOCA (P26484) FixC protein
Length = 435
Score = 39.7 bits (91), Expect = 0.001
Identities = 18/35 (51%), Positives = 22/35 (62%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
K D ++VG+G G A LA GLKVL LE+G Y
Sbjct: 5 KFDAIVVGAGMAGNAATLTLARRGLKVLQLERGEY 39
>CRTI_PHYBL (P54982) Phytoene dehydrogenase (EC 1.14.99.-) (Phytoene
desaturase)
Length = 583
Score = 38.9 bits (89), Expect = 0.002
Identities = 17/32 (53%), Positives = 23/32 (71%)
Query: 75 VVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
V+I+G+G GG AA LA G+KV V+EK N+
Sbjct: 7 VIIIGAGAGGTATAARLAREGIKVTVVEKNNF 38
>FIXC_AZOVI (P53572) FixC protein
Length = 427
Score = 38.5 bits (88), Expect = 0.003
Identities = 17/31 (54%), Positives = 22/31 (70%)
Query: 74 DVVIVGSGCGGGVAAAVLASSGLKVLVLEKG 104
DV++VG+G G AA LA GLKVL +E+G
Sbjct: 6 DVIVVGAGMAGNAAAYTLAKGGLKVLQIERG 36
>AOFB_RAT (P19643) Amine oxidase [flavin-containing] B (EC 1.4.3.4)
(Monoamine oxidase) (MAO-B)
Length = 519
Score = 38.1 bits (87), Expect = 0.004
Identities = 19/44 (43%), Positives = 26/44 (58%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSSR 115
KCDV++VG G G AA +L GL V+VLE + R ++ R
Sbjct: 3 KCDVIVVGGGISGMAAAKLLHDCGLSVVVLEARDCVGGRTYTIR 46
>DLDH_STAAM (Q59822) Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (E3
component of pyruvate complex) (Dihydrolipoamide
dehydrogenase) (Membrane-bound ribosome protein complex
50 kDa subunit)
Length = 468
Score = 37.0 bits (84), Expect = 0.008
Identities = 16/35 (45%), Positives = 24/35 (67%)
Query: 71 VKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGN 105
++ D +++G+G GG VAA A G KV ++EKGN
Sbjct: 8 IETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGN 42
>THI4_METJA (Q58018) Putative thiazole biosynthetic enzyme
Length = 263
Score = 36.6 bits (83), Expect = 0.010
Identities = 21/62 (33%), Positives = 33/62 (52%), Gaps = 6/62 (9%)
Query: 42 MNEDDLSLAQSLSNKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVL 101
+N D+ +++ ++ +D V+ DVVIVG+G G A LA G KV+VL
Sbjct: 8 LNADETKTTKAILKASFDMWLDI------VEADVVIVGAGPSGLTCARYLAKEGFKVVVL 61
Query: 102 EK 103
E+
Sbjct: 62 ER 63
>THI4_ARCFU (O29556) Putative thiazole biosynthetic enzyme
Length = 260
Score = 36.6 bits (83), Expect = 0.010
Identities = 25/73 (34%), Positives = 34/73 (46%), Gaps = 21/73 (28%)
Query: 31 ERPLEKGIVEAMNEDDLSLAQSLSNKGLEIDIDANSNILKVKCDVVIVGSGCGGGVAAAV 90
E + K IVE +E+ + A+S DV++VG+G G AA
Sbjct: 10 EAEITKAIVETASEEWVEYAES---------------------DVIVVGAGPSGLTAARY 48
Query: 91 LASSGLKVLVLEK 103
LA GLK LVLE+
Sbjct: 49 LAEKGLKTLVLER 61
>THI4_AERPE (Q9Y9Z0) Putative thiazole biosynthetic enzyme
Length = 274
Score = 36.6 bits (83), Expect = 0.010
Identities = 16/36 (44%), Positives = 26/36 (71%)
Query: 71 VKCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNY 106
++ DV++VG+G G AA LA +G +VL++E+ NY
Sbjct: 31 LRSDVIVVGAGPAGLTAAWRLAEAGARVLIVEQNNY 66
>MDL2_PRUSE (O50048) (R)-mandelonitrile lyase 2 precursor (EC
4.1.2.10) (Hydroxynitrile lyase 2) ((R)-oxynitrilase 2)
Length = 576
Score = 36.2 bits (82), Expect = 0.014
Identities = 31/80 (38%), Positives = 39/80 (48%), Gaps = 9/80 (11%)
Query: 48 SLAQSLSNKG------LEIDIDANSNILKVKCDVVIVGSGCGGGVAAAVLASSGLKVLVL 101
S QSL+N LE DAN L+ D +IVG G G AA L S+ VLVL
Sbjct: 24 SEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATL-SANYSVLVL 82
Query: 102 EKGNYFT--PRDFSSRRFLF 119
E+G T P +S F++
Sbjct: 83 ERGTLPTEYPNLLTSDGFIY 102
>FIG1_HUMAN (Q96RQ9) Interleukin-4 induced protein 1 precursor
(Fig-1 protein) (hFIG1) (UNQ636/PRO1265)
Length = 567
Score = 36.2 bits (82), Expect = 0.014
Identities = 19/41 (46%), Positives = 26/41 (63%)
Query: 75 VVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPRDFSSR 115
V++VG+G G VAA VL+ +G KV +LE N R F+ R
Sbjct: 62 VIVVGAGVAGLVAAKVLSDAGHKVTILEADNRIGGRIFTYR 102
>YOR3_METBA (P96800) Hypothetical 31.9 kDa protein in HDRD 3'region
(ORF3)
Length = 282
Score = 35.8 bits (81), Expect = 0.018
Identities = 20/39 (51%), Positives = 26/39 (66%), Gaps = 2/39 (5%)
Query: 72 KCDVVIVGSGCGGGVAAAVLASSGLKVLVLEKGNYFTPR 110
K DVVI+G+G G AA +LA S + VLV++K Y PR
Sbjct: 6 KYDVVIIGAGPAGSYAAYMLAKSKINVLVIDK--YSFPR 42
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.313 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,507,510
Number of Sequences: 164201
Number of extensions: 562154
Number of successful extensions: 2486
Number of sequences better than 10.0: 338
Number of HSP's better than 10.0 without gapping: 285
Number of HSP's successfully gapped in prelim test: 53
Number of HSP's that attempted gapping in prelim test: 2179
Number of HSP's gapped (non-prelim): 356
length of query: 119
length of database: 59,974,054
effective HSP length: 95
effective length of query: 24
effective length of database: 44,374,959
effective search space: 1064999016
effective search space used: 1064999016
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 58 (26.9 bits)
Medicago: description of AC144481.8