BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC144477.8 + phase: 0 /pseudo
(879 letters)
Database: sprot
164,201 sequences; 59,974,054 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog 87 3e-16
MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250... 84 1e-15
POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains... 81 1e-14
LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog 77 3e-13
YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein ... 67 2e-10
PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type... 60 3e-08
PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type... 50 2e-05
PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type... 48 1e-04
POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type... 45 6e-04
YO84_CAEEL (P34620) Hypothetical protein ZK1236.4 in chromosome III 37 0.23
CAPG_HUMAN (P40121) Macrophage capping protein (Actin-regulatory... 37 0.30
CAPG_MOUSE (P24452) Macrophage capping protein (Myc basic motif ... 35 0.67
RRPL_PI3H4 (P12577) RNA polymerase beta subunit (EC 2.7.7.48) (L... 32 5.6
YMH5_CAEEL (P34472) Hypothetical protein F58A4.5 in chromosome III 32 9.6
>LIN1_NYCCO (P08548) LINE-1 reverse transcriptase homolog
Length = 1260
Score = 86.7 bits (213), Expect = 3e-16
Identities = 74/300 (24%), Positives = 130/300 (42%), Gaps = 12/300 (4%)
Query: 58 IAFVSGRSILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMR 117
+ F+ G N +I +I ++ +K+K K L +D K +D I ++ + K+
Sbjct: 563 VGFIPGSQGWFNIRKSINVIQHI-NKLKNKD-HMILSIDAEKAFDNIQHPFMIRTLKKIG 620
Query: 118 FSDQWVKWIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRK 177
++K I +++NG + G RQG PLSP LF I +E L+ +R+
Sbjct: 621 IEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIRE 680
Query: 178 DEARGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQ 237
++A I G+ I + +S LFADD ++ D +K+ ++ Y G +N
Sbjct: 681 EKA---IKGIHIGSEEIKLS--LFADDMIVYLENTRDSTTKLLEVIKEYSNVSGYKINTH 735
Query: 238 KSEIFCSRNVLTTVHNNIASILGVQVVIGTGKYLG--LPSMIGRSKKSTFSFIKDRIWKK 295
KS F N + + VV KYLG L + K + ++ I +
Sbjct: 736 KSVAFIYTN-NNQAEKTVKDSIPFTVVPKKMKYLGVYLTKDVKDLYKENYETLRKEIAED 794
Query: 296 INLWSSKSLSKAGREVLIKFVL--QSIPTYFMSLFTHPVSLCDEIERIMNSFWWGHSGGQ 353
+N W + S GR ++K + ++I + P+S ++E+I+ F W Q
Sbjct: 795 VNKWKNIPCSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKIILHFIWNQKKPQ 854
>MC50_ARATH (P92555) Hypothetical mitochondrial protein AtMg01250
(ORF102)
Length = 122
Score = 84.3 bits (207), Expect = 1e-15
Identities = 37/67 (55%), Positives = 50/67 (74%)
Query: 138 LVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIIS 197
++NG + P RGLRQGDPLSPYLFI+C E LS L R+ + +G + G+++ NN+P I+
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72
Query: 198 HLLFADD 204
HLLFADD
Sbjct: 73 HLLFADD 79
>POL2_MOUSE (P11369) Retrovirus-related Pol polyprotein [Contains:
Reverse transcriptase (EC 2.7.7.49); Endonuclease]
Length = 1300
Score = 81.3 bits (199), Expect = 1e-14
Identities = 73/297 (24%), Positives = 126/297 (41%), Gaps = 14/297 (4%)
Query: 58 IAFVSGRSILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMR 117
+ F+ G N +I +IHY+ +K+K K + LD K +D I ++ V+ +
Sbjct: 590 VGFIPGMQGWFNIRKSINVIHYI-NKLKDKN-HMIISLDAEKAFDKIQHPFMIKVLERSG 647
Query: 118 FSDQWVKWIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRK 177
++ I + VNG+ + I G RQG PLSPYLF I +E L+ +R+
Sbjct: 648 IQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQ 707
Query: 178 DEARGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQ 237
+ EI G++I IS L ADD ++ + ++ +++++ G +N
Sbjct: 708 QK---EIKGIQIGKEEVKIS--LLADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSN 762
Query: 238 KSEIFC-SRNVLTTVHNNIASILGVQVVIGTGKYLG--LPSMIGRSKKSTFSFIKDRIWK 294
KS F ++N I +V KYLG L + F +K I +
Sbjct: 763 KSMAFLYTKN--KQAEKEIRETTPFSIVTNNIKYLGVTLTKEVKDLYDKNFKSLKKEIKE 820
Query: 295 KINLWSSKSLSKAGREVLIKFVL--QSIPTYFMSLFTHPVSLCDEIERIMNSFWWGH 349
+ W S GR ++K + ++I + P +E+E + F W +
Sbjct: 821 DLRRWKDLPCSWIGRINIVKMAILPKAIYRFNAIPIKIPTQFFNELEGAICKFVWNN 877
>LIN1_HUMAN (P08547) LINE-1 reverse transcriptase homolog
Length = 1259
Score = 76.6 bits (187), Expect = 3e-13
Identities = 64/259 (24%), Positives = 117/259 (44%), Gaps = 10/259 (3%)
Query: 93 LKLDISKTYDMIDWSYLRGVMTKMRFSDQWVKWIMMCVETVNYFVLVNGDVISQIIPGRG 152
+ +D K +D I ++ + K+ ++K I + +++NG + G
Sbjct: 596 ISIDAEKAFDKIQQPFMLKPLNKLGIDGTYLKIIRAIYDKPTANIILNGQKLEAPPLKTG 655
Query: 153 LRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLFFRTN 212
RQG PLSP L I +E L+ +R+++ EI G+++ +S LFADD ++
Sbjct: 656 TRQGCPLSPLLPNIVLEVLARAIRQEK---EIKGIQLGKEEVKLS--LFADDMIVYLENP 710
Query: 213 VDQASKMRAILSTYERAYGQAVNLQKSEIFCSRNVLTTVHNNIASILGVQVVIGTGKYLG 272
+ A + ++S + + G +N+QKS+ F N T + I S L + KYLG
Sbjct: 711 IVSAQNLLKLISNFSKVSGYKINVQKSQAFLYTNNRQT-ESQIMSELPFTIASKRIKYLG 769
Query: 273 --LPSMIGRSKKSTFSFIKDRIWKKINLWSSKSLSKAGREVLIKF-VLQSIPTYFMSL-F 328
L + K + + + I + N W + S GR ++K +L + F ++
Sbjct: 770 IQLTRDVKDLFKENYKPLLNEIKEDTNKWKNIPCSWVGRINIVKMAILPKVIYRFNAIPI 829
Query: 329 THPVSLCDEIERIMNSFWW 347
P++ E+E+ F W
Sbjct: 830 KLPMTFFTELEKTTLKFIW 848
>YTX2_XENLA (P14381) Transposon TX1 hypothetical 149 kDa protein
(ORF 2)
Length = 1308
Score = 67.0 bits (162), Expect = 2e-10
Identities = 70/291 (24%), Positives = 123/291 (42%), Gaps = 16/291 (5%)
Query: 61 VSGRSILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRFSD 120
V GR+I DN +++H+ + G F L LD K +D +D YL G + F
Sbjct: 562 VPGRTIFDNVFLIRDLLHFARRT--GLSLAF-LSLDQEKAFDRVDHQYLIGTLQAYSFGP 618
Query: 121 QWVKWIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEA 180
Q+V ++ + V +N + + + GRG+RQG PLS L+ + +E L+RK
Sbjct: 619 QFVGYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLRK--- 675
Query: 181 RGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSE 240
+ G+ + + +ADD L + VD + + Y A +N KS
Sbjct: 676 --RLTGLVLKEPDMRVVLSAYADDVILVAQDLVD-LERAQECQEVYAAASSARINWSKSS 732
Query: 241 IFCSRNV-LTTVHNNIASILGVQVVIGTGKYLGL-PSMIGRSKKSTFSFIKDRIWKKINL 298
++ + + I +I KYLG+ S F +++ + ++
Sbjct: 733 GLLEGSLKVDFLPPAFRDISWESKII---KYLGVYLSAEEYPVSQNFIELEECVLTRLGK 789
Query: 299 WS--SKSLSKAGREVLIKFVLQSIPTYFMSLFTHPVSLCDEIERIMNSFWW 347
W +K LS GR ++I ++ S Y + + +I+R + F W
Sbjct: 790 WKGFAKVLSMRGRALVINQLVASQIWYRLICLSPTQEFIAKIQRRLLDFLW 840
>PO23_POPJA (Q05118) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 606
Score = 60.1 bits (144), Expect = 3e-08
Identities = 61/228 (26%), Positives = 99/228 (42%), Gaps = 23/228 (10%)
Query: 74 IEIIHYMK-SKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRFSDQWVKWIMMCVET 132
I + HY+K ++KGK + + LDI K +D + + M D +IM +
Sbjct: 7 IMLEHYIKLRRLKGKTYN-VVSLDIRKAFDTVSHPAILRAMRAFGIDDGMQDFIMSTITD 65
Query: 133 VNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNN 192
++V G ++I G++QGDPLSP LF I ++ L + R ++ + + C
Sbjct: 66 AYTNIVVGGRTTNKIYIRNGVKQGDPLSPVLFNIVLDEL--VTRLNDEQPGASMTPACK- 122
Query: 193 APIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKSEIFCSRNV----- 247
I+ L FADD L ++D + + A Y R G +N +K + V
Sbjct: 123 ---IASLAFADDLLLLEDRDIDVPNSL-ATTCAYFRTRGMTLNPEKCASISAATVSGRSV 178
Query: 248 -----LTTVHNNIASILGVQVVIGTGKYLGLP-SMIGRSKKSTFSFIK 289
T+ LG I T KYLGL S G +K + ++ +
Sbjct: 179 PRSKPSFTIDGRYIKPLG---GINTFKYLGLTFSSTGVAKPTVYNLTR 223
>PO21_NASVI (Q03278) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 1025
Score = 50.4 bits (119), Expect = 2e-05
Identities = 44/156 (28%), Positives = 70/156 (44%), Gaps = 17/156 (10%)
Query: 59 AFVSGRSILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRF 118
AF+ + +NT +I + K+KG + LD+ K +D ++ + + + +
Sbjct: 422 AFIVADGVAENTSLLSAMIKEARMKIKGL---YIAILDVKKAFDSVEHRSILDALRRKKL 478
Query: 119 SDQ---WVKWIMMCVETVNYFVLVNGDVISQIIPGRGLRQGDPLSPYLFIICVEGLSALV 175
+ ++ W+ +T V G I P RG+RQGDPLSP LF ++ + L
Sbjct: 479 PLEMRNYIMWVYRNSKTRLEVVKTKGRWIR---PARGVRQGDPLSPLLFNCVMD--AVLR 533
Query: 176 RKDEARGEINGVKICNNAPIISHLLFADDCFLFFRT 211
R E G + G A I L+FADD L T
Sbjct: 534 RLPENTGFLMG------AEKIGALVFADDLVLLAET 563
>PO22_POPJA (Q03274) Retrovirus-related Pol polyprotein from type I
retrotransposable element R2 [Contains: Reverse
transcriptase (EC 2.7.7.49); Endonuclease] (Fragment)
Length = 711
Score = 48.1 bits (113), Expect = 1e-04
Identities = 47/180 (26%), Positives = 86/180 (47%), Gaps = 11/180 (6%)
Query: 63 GRSILDNTM-DAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRFSDQ 121
G + +D T+ +++ + Y+ S+ + +K + LD+ K +D + S + + ++ +
Sbjct: 109 GYARIDGTLVNSLLLDTYISSRREQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEG 168
Query: 122 WVKWIMMCVETVNYFVLVN-GDVISQIIPGRGLRQGDPLSPYLF-IICVEGLSALVRKDE 179
+I + + V G +I RG++QGDPLSP+LF + E L +L
Sbjct: 169 TSNYITGSLSDSTTTIRVGPGSQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQSTPG 228
Query: 180 ARGEINGVKICNNAPIISHLLFADDCFLFFRTNVDQASKMRAILSTYERAYGQAVNLQKS 239
G I KI P+ L FADD L +V + + A ++ + R G ++N +KS
Sbjct: 229 IGGTIGEEKI----PV---LAFADDLLLLEDNDVLLPTTL-ATVANFFRLRGMSLNAKKS 280
>POLR_DROME (P16423) Retrovirus-related Pol polyprotein from type II
retrotransposable element R2DM [Contains: Protease (EC
3.4.23.-); Reverse transcriptase (EC 2.7.7.49);
Endonuclease]
Length = 1057
Score = 45.4 bits (106), Expect = 6e-04
Identities = 32/123 (26%), Positives = 56/123 (45%), Gaps = 8/123 (6%)
Query: 91 FALKLDISKTYDMIDWSYLRGVMTKMRFSDQWVKWIMMCVETVNYFVLVNGDVISQIIPG 150
+ LD+SK +D + + + + +V ++ E + +G + +P
Sbjct: 477 YIANLDVSKAFDSLSHASIYDTLRAYGAPKGFVDYVQNTYEGGGTSLNGDGWSSEEFVPA 536
Query: 151 RGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLFADDCFLFFR 210
RG++QGDPLSP LF + ++ L + + G K+ N I + FADD LF
Sbjct: 537 RGVKQGDPLSPILFNLVMDRLLRTLPSE------IGAKVGN--AITNAAAFADDLVLFAE 588
Query: 211 TNV 213
T +
Sbjct: 589 TRM 591
>YO84_CAEEL (P34620) Hypothetical protein ZK1236.4 in chromosome III
Length = 364
Score = 37.0 bits (84), Expect = 0.23
Identities = 61/262 (23%), Positives = 102/262 (38%), Gaps = 34/262 (12%)
Query: 95 LDISKTYDMIDWSYLRGVMTKMRFSDQWVKWIMMCVETVNYFVLVNGDVISQIIPGRGLR 154
LD SK +D + L +T ++ + ++W+ + + ++ V V + G+
Sbjct: 23 LDFSKAFDKVSHDILLDKLTSIKINKHLIRWLDVFLTNRSFKVKVGNTLSEPKKTVCGVP 82
Query: 155 QGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPI-ISHLLFADDCFLF--FRT 211
QG +SP LF I V +SA N P+ + FADD L+
Sbjct: 83 QGSVISPVLFGIFVNEISA------------------NLPVGVYCKQFADDIKLYAAIPK 124
Query: 212 NVDQASKMRAILSTYERAYGQAVNLQKSEIF------CSRNVLTTVHNNIASILGVQVVI 265
N Q S AI + A + L S+ F C+ N T N I +
Sbjct: 125 NQSQISLQLAIDVVTDWALNNKLLLNPSKTFHLTLGKCNTNY--TYFLNGLPIKKEVLTR 182
Query: 266 GTGKYLGLPSMIGRSKKSTFSFIKDRIWKKINLWSSKSLSKAGREVLIKFVLQSIPTYFM 325
G + K T + K ++++ N +SSKS+ +L K ++ Y
Sbjct: 183 DLGFLVSEKLDFTDHWKKTINLTKYQLFQIFNTYSSKSVKL--MTLLYKTFIRPRLEY-G 239
Query: 326 SLFTHPVSLCDE--IERIMNSF 345
++ T P+ D IE + N+F
Sbjct: 240 TVITSPIKKNDSKAIESVQNAF 261
>CAPG_HUMAN (P40121) Macrophage capping protein (Actin-regulatory
protein CAP-G)
Length = 348
Score = 36.6 bits (83), Expect = 0.30
Identities = 18/66 (27%), Positives = 35/66 (52%)
Query: 142 DVISQIIPGRGLRQGDPLSPYLFIICVEGLSALVRKDEARGEINGVKICNNAPIISHLLF 201
++I + P L++G+P +AL + +A G++N K+ +++P LL
Sbjct: 219 EMIQVLGPKPALKEGNPEEDLTADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLI 278
Query: 202 ADDCFL 207
+DDCF+
Sbjct: 279 SDDCFV 284
>CAPG_MOUSE (P24452) Macrophage capping protein (Myc basic motif
homolog-1) (Actin-capping protein GCAP39)
Length = 352
Score = 35.4 bits (80), Expect = 0.67
Identities = 14/36 (38%), Positives = 24/36 (65%)
Query: 172 SALVRKDEARGEINGVKICNNAPIISHLLFADDCFL 207
+AL + +A G++N K+ +++P S LL DDCF+
Sbjct: 253 AALYKVSDATGQMNLTKVADSSPFASELLIPDDCFV 288
>RRPL_PI3H4 (P12577) RNA polymerase beta subunit (EC 2.7.7.48)
(Large structural protein) (L protein)
Length = 2233
Score = 32.3 bits (72), Expect = 5.6
Identities = 19/57 (33%), Positives = 26/57 (45%)
Query: 645 ILSSKHYMIYLRTMHLYFVVCCGVYGNKETTKCGMR*LMLQFMLSREHKLWSMIDKM 701
IL ++Y YL T L C V G + C LQ M + + LW +IDK+
Sbjct: 220 ILDKQNYNGYLITPELVLPYCDVVEGRWNISACAKLDPKLQSMYQKGNNLWEVIDKL 276
>YMH5_CAEEL (P34472) Hypothetical protein F58A4.5 in chromosome III
Length = 1222
Score = 31.6 bits (70), Expect = 9.6
Identities = 27/113 (23%), Positives = 45/113 (38%), Gaps = 4/113 (3%)
Query: 60 FVSGRSILDNTMDAIEIIHYMKSKVKGKKGDFALKLDISKTYDMIDWSYLRGVMTKMRFS 119
F++ RS + + +I + H S +K +K L D +K +D + L +
Sbjct: 721 FLNFRSCPSSLVRSISLYH---SILKNEKSLDILFFDFAKAFDKVSHPILLKKLALFGLD 777
Query: 120 DQWVKWIMMCVETVNYFVLVNGDVISQIIP-GRGLRQGDPLSPYLFIICVEGL 171
W + + V +N V S P G+ QG P LFI+ + L
Sbjct: 778 KLTCSWFKEFLHLRTFSVKINKFVSSNAYPISSGVPQGSVSGPLLFILFINDL 830
Database: sprot
Posted date: Nov 25, 2004 10:54 AM
Number of letters in database: 59,974,054
Number of sequences in database: 164,201
Lambda K H
0.354 0.159 0.546
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,010,252
Number of Sequences: 164201
Number of extensions: 3119853
Number of successful extensions: 14336
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 14317
Number of HSP's gapped (non-prelim): 18
length of query: 879
length of database: 59,974,054
effective HSP length: 119
effective length of query: 760
effective length of database: 40,434,135
effective search space: 30729942600
effective search space used: 30729942600
T: 11
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.5 bits)
S2: 70 (31.6 bits)
Medicago: description of AC144477.8